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7 Abstract The thesis is focused on the pollen of selected allergenic plants. Thesis is structured into two parts., pollination and fertilization , development of the pollen grain , its structure and chemical composition. The end of the theoretical part, dedicated to allergies, is divided into subsections dealing with First, teoretical part describes the flower and its morphology, discusses the pollen grain, stamen anatomical structure immunity and allergies, naming and registration of allergens and cross- pollen allergy . The practical part contains an analysis of curriculum for students of secondary schools. An integral part of the work is a survey of teachers in secondary schools, in which method of questioning was used. The aim of this questioning was to determine the presence of solved issue in terms of representation of pollen and pollen allergens in textbooks for secondary schools , as well as the inclusion of this issue in the official curriculum, under the National Programme for the Development of Education in the Czech Republic. Based on the results of a questionnaire survey of secondary school teachers, teaching materials have been developed in the form of two worksheets for teaching botany and guidance on laboratory exercises in plant biology.

Orientadores: Maria Helena Andrade Santana, Ricardo de Lima Zollner / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Quimica / Made available in DSpace on 2018-07-27T03:55:49Z (GMT). No. of bitstreams: 1 Victorino_IgorRicardodeSouza_M.pdf: 6760377 bytes, checksum: 05fc12e1f97a8b092dbd91b47b06441d (MD5) Previous issue date: 2000 / Resumo: Os lipossomas têm sido utilizados como imunoadjuvantes antigênicos, em estudos que enfatizam a sua atuação no sistema imunológico. Extratos peptídicos ou protéicos de várias fontes foram encapsulados em lipossomas ou associados à sua superficie, e aplicados em imunoterapias e vacinas. Apesar disso, poucos são os estudos voltados para a performance do processo de preparação desses lipossomas, eficiência da associação proteína/lipídio e estabilidade das vesículas. Este trabalho trata da avaliação do encapsulamento das proteínas: Albumina de Soro Bovino (BSA), Mioglobina (Mio) e Cito cromo C (Cit C), simulando extratos alergênicos provenientes de fungos e ácaros, no que se refere à faixa de peso molecular das suas proteínas. Os lipossomas foram preparados com os lipídios L-a-distearoilfosfatidi1colina (DSPC) e Colesterol (Col) na razão molar 70:30 respectivamente. As proteínas foram encapsuladas individualmente e em forma de misturas de composições molares BSA:Mio:Cit C de 20:40:40, 40:20:40 e 40:40:20, respectivamente. Além disso, a mistura de proteínas de composição 40:20:40 (BSA:Mio:Cit C) foi também associada covalentemente à superficie das vesículas. Os lipossomas foram caracterizados pelo teor de fósforo e diâmetro médio. O desempenho do encapsulamento foi analisado através dos perfis e eficiências de encapsulamento das proteínas individuais e em misturas, além da estabilidade das vesículas em tensoativo penta etileno glicol mono-n-dodecil éter (C12Es), e de plasma humano. Para as misturas, analisou-se a exclusão de proteínas durante o encapsulamento e a associação à superficie dos lipossomas. Os resultados indicam que a eficiência de encapsulamento depende mais das interações proteína:lipídio que do tamanho das proteínas. As eficiências de encapsulamento variaram entre 0,03% e 3,55% para as proteínas individuais e entre 1,04% e 3,94% para as misturas. Não houve alteração na estabilidade dos lipossomas em C12Es com a presença das proteínas. Em plasma, essas vesículas permaneceram estáveis por aproximadamente 20 horas. Na associação à superficie dos lipossomas, predominou a presença de BSA em relação às outras proteínas / Abstract: Liposomes has been studied as antigenic immunoadjuvants with emphasis on their performance in the imunological system. Peptides or proteins of various sources were encapsulated in liposomes or associated to their surface, and applied in immunotherapy and vaccines. In spite of this, there are few studies about the performance of preparation process ofthese liposomes, efficacy ofthe association proteinllipid and the stability ofvesic1es. This work concems with the evaluation of the encapsulation of the proteins Bovine Serum Albumin (BSA), Myoglobin (Myo) and Cythochrome C (Cyt C), simulating allergen extracts from molds and mites, related to the range of molecular weight of their proteins. Liposomes were prepared with lipid L-a-disteraoylphosphatidylcholine (DSPC) and Cholesterol (Chol) at molar ratio 70:30 respectively. The proteins were encapsulated individualyand in mixtures with molar composition BSA:Myo:Cyt C of20:40:40, 40:20:40 and 40:40:20 respectively. Furthermore, the mixture 40:20:40 (BSA:Myo:Cyt C) was also covalent1y associated to the surface of vesic1es. Liposomes were characterized by their phosphate contents and mean diameter. The performance of the protein encapsulation was evaluated through the profiles and efficiency of the encapsulation and throught the stability of vesic1es in the presence of the surfactant penta ethylene glycol mono-n-dodecyl ether (C12ES) and human plasma. For the mixtures, the exclusion of proteins duringentrapment or association to the liposome surface was also evaluated. The experimental results indicate that the efficacy of encapsulation is more dependent of the interactions proteinllipid than the size of the proteins. The efficiencies of encapsulation changed between 0.03% and 3,55% for individual proteins and were between 1,04% and 3,94% for the mixtures. The presence ofproteins does not altered the stability of liposomes in C12ES . In human plasma the vesic1es remained with stability about 20 hours. For the mixtures, the presence ofthe BSA protein predominated in the vesicles / Mestrado / Desenvolvimento de Processos Biotecnologicos / Mestre em Engenharia Química

This thesis deals with the assessment of aroma active compounds in non-chocolate confectionery and their influence on sensory quality. In the theoretical part the production technology of non-chocolate confectionery from raw materials to final product is described. Furthermore, aroma active substances, including those potentially allergenic, are characterized, and the method for their determination by solid phase microextraction and gas chromatography with mass spectrometry is briefly described. Sensory analysis and sensory evalutation methods are briefly desribed. Within expermental part of the work selected parametres of the HS-SPME-GC-MS method were optimized, aroma compounds in samples of hard candies were identified, and 24 potentially allergenic aroma substances were identified and quantified. Finally, a sensory analysis of these samples was performed. Differences in the number and type of volatile compounds were found between the samples. Overall 393 compounds were indetified including 19 allergens.

This diploma thesis focuses on identification of aroma active substances and sensory analysis in samples of non-chocolate sweets. For experimental part jelly candies were chosen as model samples. Specifically gummy bears with strawberry, lemon, apple and orange flavour were used. The theoretical part deals with the legislation and technology of manufacturing non-chocolate sweets, characterization of aroma compounds including those which could be allergenic. Furthermore this thesis describes a method of their determination using solid phase microextraction and gas chromatography with mass spectrometry. The end of the theoretical part is dedicated to methods of sensory analysis that were used. In the experimental part, the chosen method HS-SPME-GC-MS was used to identify volatile aroma active substances in selected samples of jelly sweets. The substances that are potentially allergenic were also quantified. For the evaluation of sensory quality scales, profile test and overall acceptability were used. The single samples differ in number and type of volatile compounds depending on producer and flavour. Altogether 119 compounds were identified including 12 allergenic.

In my thesis I deal with the pollen of allergenic plants, the origin and proces of allergy to plant pollen and the inclusion of these topics in the teaching of plant biology in high schools. In the theoretical part I focus on topic of pollen allergy. First, I generally describe allergy and its origin, causes of allergic diseases, environmental factors and the development of allergic disease. I also briefly address the naming and registration of allergens and I also mention the so-called cross-allergy. Great emphasis is placed on pollen allergy, which is devoted to a larger unit, divided into individual subchapters dealing with the physiological function of the nose, followed by an allergic reaction of type I, an allergic reaction that occurs on the nasal mucosa. Furthermore, subchapters mentioning the classification of allergic rhinitis, diagnosis and pharmacotherapy, including additions to the drug groups used in the treatment of pollen allergy. Another larger unit is devoted to the structure of the plant's blossom as a source of allergen, which includes the anatomy of the blossom parts and a description of the pollen grain. The anatomical description of the blossom is followed by a practical part, first created by a tabular overview with selected allergenic plants. Each plant causing the allergy...

Submitted by Erika Demachki (erikademachki@gmail.com) on 2015-01-30T15:53:39Z No. of bitstreams: 2 Dissertação - Moisés Queiroz Resende - 2014.pdf: 891007 bytes, checksum: 48a7b34dc669b45819e19f6d364dfdb5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Erika Demachki (erikademachki@gmail.com) on 2015-01-30T15:54:22Z (GMT) No. of bitstreams: 2 Dissertação - Moisés Queiroz Resende - 2014.pdf: 891007 bytes, checksum: 48a7b34dc669b45819e19f6d364dfdb5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2015-01-30T15:54:22Z (GMT). No. of bitstreams: 2 Dissertação - Moisés Queiroz Resende - 2014.pdf: 891007 bytes, checksum: 48a7b34dc669b45819e19f6d364dfdb5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-08-29 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / ABSTRACT: Two experiments were conduct to evaluate the effect of using soy protein concentrate (SPC) in pre-starter piglet diets over performance, intestinal histomofometria and blood parameters. In the first trial 72 piglets weaned at 21 days of age were used and 54 for the second trial. Both were conducted as a randomized blocks design with six replications and three piglets per unit. The first trial had four diets (0% SPC, 3% SPC, 6% SPC and 9% SPC), which evaluated different levels of SPC inclusion in diets, and the second trial, which evaluated the replacement of spray dried blood plasma (SDP) by SPC, had three diets (0.0%-SPC + 5.0-SDP, 2.5%-SPC + 2.5%-SDP and 5.0%-SPC + 0.0%-SDP). In both trials, diets and water were offered ad libitum in pre-starter l (21-32 days old) and pre-starter ll (33-42 days old) phases, but during the starter phase (43-66 days old) all piglets received a single diet. At 32 days of age, blood was collected from one animal per experimental unit and then these animals were euthanized to collect samples of the small intestine. Linear effect was observed In the first trial, over feed conversion (FC) as the level of CPS in diet was increased in the period between 21 and 32 days of age. Linear effect was also observed on the FC in the period of 33-42 days old, however in a reverse form from the previous period, wherein the increase of the SPC level in diets resulted in the increase of FC. In the total period of this trial (21-66 days old), it was found a quadratic effect of SPC on piglets FC. In the second trial, the replacement of SDP by CPS caused effect on performance variables, except for feed conversion (FC) during 21-32 days of piglets age. The average daily feed intake (ADFI) was higher for pigs fed 2.5%-SPC + 2.5%-SDP and the average daily gain (ADG) and the final body weight (FBW) of the piglets in this period was higher for 2.5%-SPC + 2.5%-SDP compared to 5.0%-SPC + 0.0%-SDP. In the period of 21-42 days old, it was observed best results of ADFI and FBW for piglets fed diet containing 2.5%-SPC + 2.5%-SDP compared to piglets fed 5.0%-SPC + 0.0%-SDP. The ADG showed better results for diets containing 0.0%-SPC + 5,0-SDP and 2.5%-SPC + 2.5%-SDP and FC was higher for the diet with 0.0%-SPC + 5.0%-SDP compared to the diet with 5.0%-SPC + 0.0%-SDP. During total period of the experiment (21-66 days old) ADG and ADFI was influenced by substitution of SDP for SPC, wherein the best results was presented by piglets fed 2.5%-SPC + 2.5%-SDP compared to those fed 5.0%-SPC + 0.0%-SDP. In both trials, no effect was observed upon histomorphometric variables (villus height, crypt depth and villus: crypt). Regarding the blood variables (leukocytes, eosinophils and lymphocytes), no effect of SPC at 32 days of age the piglets was observed in the first trial. But in the second trial, total leukocyte count was higher in animals fed 5.0%-SPC + 0.0%-SDP compared to ones fed 0.0%-SPC + 5.0%-SDP and lymphocyte count was lower in piglets receiving 0.0%-SPC + 5.0%-SDP. In the first trial, the use of SPC in diets of post-weaning piglets during pre-starter period (21-42 days old) did not influence performance from 21 to 66 days of life, or intestinal morphology and leukocyte, lymphocytes and eosinophils count in these animals. In the second, the combined use of SPC and SDP in the diets of pigs between 21 and 42 days of age reduces the activation of the immune system and improves productive performance. / Foram conduzidos dois experimentos com objetivo de avaliar o efeito da utilização de Concentrado proteico de soja (CPS) em dietas pré-inicias de leitões sobre o desempenho, histomofometria intestinal e alguns parâmetros sanguíneos. Foram utilizados 72 leitões machos castrados desmamados aos 21 dias de idade no primeiro experimento e 54 no segundo. Ambos foram delineados em blocos ao acaso com seis repetições e três leitões por unidade experimental, tendo o primeiro quatro tratamentos (0% CPS, 3% CPS, 6% CPS e 9% CPS), o qual avaliou diferentes níveis de inclusão de CPS nas dietas, e o segundo, para avaliar a substituição de plasma sanguíneo (SDP) por CPS, três (0,0%-CPS + 5,0-SDP, 2,5%-CPS + 2,5%-SDP e 5,0%-CPS + 0,0%-SDP). Em ambos as rações experimentais e água foram fornecidas à vontade nas fases pré-inicial l (21 a 32 dias de idade) e pré-inicial ll (33 a 42 dias de idade), sendo que na fase inicial (43 a 66 dias de idade) todos os leitões receberam uma única dieta. Aos 32 dias de idade foi coletado sangue de um animal por unidade experimental e em seguida estes animais foram eutanasiados para coleta de amostras de intestino delgado. No primeiro experimento, foi observado efeito linear decrescente sobre a conversão alimentar (CA) à medida que aumentou o nível de CPS na dieta no período entre 21 e 32 dias de idade. Efeito linear também foi observado sobre a CA no período entre 33 e 42 dias de idade dos leitões, entretanto de forma inversa ao período anterior, ou seja, à medida que se aumentou o nível de CPS na dieta a CA aumentou. Foi observado no período total (21 a 66 dias de idade) do primeiro experimento efeito quadrático do CPS sobre a CA dos leitões. No segundo experimento a substituição de SDP por CPS causou efeito sobre as variáveis de desempenho no período entre 21 e 32 dias de idade, exceto sobre a CA. O CDR foi superior para os leitões alimentados com 2,5%-CPS + 2,5%-SDP e o GDP e o peso final (PF) dos animais neste período foi superior com 2,5%-CPS + 2,5%-SDP comparado a 5,0%-CPS + 0,0%-SDP. No período entre 21 e 42 dias de idade foi observado melhores resultados de CDR e PF para a dieta com 2,5%-CPS + 2,5%-SDP comparado a 5,0%-CPS + 0,0%-SDP. O GDP apresentou melhores resultados para as dietas com 0,0%-CPS + 5,0-SDP e 2,5%-CPS + 2,5%-SDP e a CA foi superior para a dieta com 0,0%-CPS + 5,0%-SDP diante da dieta com 5,0%-CPS + 0,0%-SDP. No período total do experimento (21 a 66 dias de idade) foi observado efeito da substituição dos SDP por CPS sobre o GDP e o CDR, sendo que os animais alimentados com 2,5%-CPS + 2,5%-SDP apresentaram resultados superiores aos alimentados com 5,0%-CPS + 0,0%-SDP. Emambos os experimentos nenhum efeito foi observado sobre as variáveis histomorfométricas (altura de vilosidade, profundidade de cripta e relação vilo:cripta). Em relação às variáveis sanguíneas (leucócitos, linfócitos e eosinófilos) do primeiro experimento, não foi observado nenhum efeito do CPS aos 32 dias de idade dos leitões. No segundo experimento, a contagem total de leucócitos foi superior para os animais alimentados com 5,0%-CPS + 0,0%-SDP sobre os alimentados com 0,0%-CPS + 5,0%-SDP e a contagem de linfócitos foi menor nos leitões que receberam 0,0%-CPS + 5,0%-SDP. No primeiro experimento, a utilização de CPS em dieta de leitões pós-desmame no período préinicial (21 a 42 dias de idade) não influenciou o desempenho produtivo dos leitões no período entre 21 e 66 dias de vida, nem a histomorfometria intestinal e a contagem de leucócitos, linfócitos e eosinófilos desses animais. No segundo, a utilização conjunta de CPS e SDP nas rações reduz a ativação do sistema imune e melhora o desempenho produtivo.

O Sistema de Análise de Perigos e Pontos Críticos de Controle (APPCC) ou Hazard Analysis Critical Control Point (HACCP) é de grande importância para a Saúde Pública, quanto às doenças veiculadas por alimentos. O objetivo deste trabalho foi verificar a aplicabilidade do uso desse sistema na indústria de moagem de trigo, no processamento de farinha de trigo integral, produto com demanda crescente no mercado. Trinta produções de farinha foram coletadas numa indústria cuja capacidade de moagem é de 15ton./h na cidade de São Paulo. A população amostrada foi de 30 sub-amostras de trigo argentino (A) (12,8% de umidade média), 17 sub-amostras de trigo brasileiro (B) (13,1% de umidade média), 30 sub-amostras de trigo preparado para moagem (M) (15,6% de umidade média) e 30 sub-amostras de farinha de trigo integral (FTI), coletadas num período de 5 meses (de outubro de 97 à março de 98). A metodologia utilizada para pesquisa de sujidades leves foi a adotada pelos Métodos Oficiais da AOAC (1995). Houve modificações no método e um estudo foi realizado para conhecer sua performance. No isolamento de fungos foi utilizada a metodologia empregada pela AOAC/FDA Bacteriological Analytical manual (1992). Para identificação de fungos em relação ao gênero foi utilizado o método preconizado GELLI et al.(1990), SAMSON et al (1995), PITT and HOCKING (1997). Na análise de sujidades leves nos trigos foi evidenciada a contaminação por ácaros (A=90%, B=53%, M=77% e FTI=53%); no trigo argentino houve uma predominância para a família Tarsonemidae, enquanto que na respectiva farinha foi a família Acaridae. Alguns ácaros dessa última família estão implicados em reações alérgicas e estão sendo tratados como emergentes na segurança alimentar. Os insetos thisanópteros, os mais freqüentes nos trigos, foram eliminados na farinha de trigo integral. O percentual médio de contaminação fúngica dos grãos foi de A=99%, B=95% e M=94%. O principal gênero encontrado foi Aspergillus spp (A= 79,0%, B= 39,3% e M= 64,2%). O menos freqüente Fusarium spp (A= 2,9%, B= 2,7% e M=1,5%). Na farinha de trigo integral o principal gênero encontrado foi o Penicillium spp e o Fusarium não foi isolado. O desoxinivalenol foi encontrado em uma amostra de farinha de trigo integral a uma concentração de 231 ng/g. O sistema APPCC se aplica aos moinhos e os auxilia a controlar suas produções do ponto de vista da segurança alimentar. / Food safety is a major concern facing the food industry today. Hazard Analysis Critical Control Point (HACCP) provides a structured approach to the assurance of safety of products. The aim of this study was to verify the use of HACCP system in a milling industry, precisely in the whole wheat flour production. At first step two hazards were analyzed: deoxynivalenol and allergenic mites in whole wheat flour Thirty (30) lots of flour were collected in an industry which capacity was 15 ton./h in São Paulo city. Those lots had 30 sub-samples of argentine (A) wheat (12,8% average moisture content), 17 sub-samples of brazilian (B) wheat (13,1% m.c.), 30 sub-samples of wheat for milling (M) (15,6% m.c), and 30 sub-samples of whole wheat flour (FTI) that were analyzed in a five months period (from 97/october to 98/march). Light filth was isolated from grains and flour, according to Official Methods of AOAC International. Methodology of extraction of light filth from whole wheat flour was studied to know the repeatability according to AOAC methods. Fungi isolation was performed according to AOAC/FDA Bacteriological Analytical Manual (1992) methodology; genera were identified according GELLI et al.(1990), SAMSON et al (1995), and PITI and HOCKING (1997). Detection and quantification of deoxynivalenol (DON) were done by thin layer chromatography (TLC) according to SABINO et al. (1989).The most significant light filth contamination in wheat were mites (A= 90%, B= 53%, M= 77% e FTI= 57%), argentine wheat had as a predominant mite the family Tarsonemidae and flour had Acaridae. The average fungal contamination in grains was 99%, 95% e 94% in argentine, brazilian and wheat for milling respectively. The predominant genera was Aspergillus spp ( A= 79,0, B=39,3% e M= 64,2 %), and Fusarium was the least frequent in wheat (A>/b>=2,7%, B= 2,8% e M=1,5%). Only one lot of whole wheat flour was contaminated by DON at level 231 ng/g. This study showed that HACCP system is appropriated for control hazards in milling industry. However, DON should be controlled before wheat get into industry, and this control need to be registered. Mites are new emergentes to cause allergy by ingestion and have to be controlled by GMP procedures.

O Sistema de Análise de Perigos e Pontos Críticos de Controle (APPCC) ou Hazard Analysis Critical Control Point (HACCP) é de grande importância para a Saúde Pública, quanto às doenças veiculadas por alimentos. O objetivo deste trabalho foi verificar a aplicabilidade do uso desse sistema na indústria de moagem de trigo, no processamento de farinha de trigo integral, produto com demanda crescente no mercado. Trinta produções de farinha foram coletadas numa indústria cuja capacidade de moagem é de 15ton./h na cidade de São Paulo. A população amostrada foi de 30 sub-amostras de trigo argentino (A) (12,8% de umidade média), 17 sub-amostras de trigo brasileiro (B) (13,1% de umidade média), 30 sub-amostras de trigo preparado para moagem (M) (15,6% de umidade média) e 30 sub-amostras de farinha de trigo integral (FTI), coletadas num período de 5 meses (de outubro de 97 à março de 98). A metodologia utilizada para pesquisa de sujidades leves foi a adotada pelos Métodos Oficiais da AOAC (1995). Houve modificações no método e um estudo foi realizado para conhecer sua performance. No isolamento de fungos foi utilizada a metodologia empregada pela AOAC/FDA Bacteriological Analytical manual (1992). Para identificação de fungos em relação ao gênero foi utilizado o método preconizado GELLI et al.(1990), SAMSON et al (1995), PITT and HOCKING (1997). Na análise de sujidades leves nos trigos foi evidenciada a contaminação por ácaros (A=90%, B=53%, M=77% e FTI=53%); no trigo argentino houve uma predominância para a família Tarsonemidae, enquanto que na respectiva farinha foi a família Acaridae. Alguns ácaros dessa última família estão implicados em reações alérgicas e estão sendo tratados como emergentes na segurança alimentar. Os insetos thisanópteros, os mais freqüentes nos trigos, foram eliminados na farinha de trigo integral. O percentual médio de contaminação fúngica dos grãos foi de A=99%, B=95% e M=94%. O principal gênero encontrado foi Aspergillus spp (A= 79,0%, B= 39,3% e M= 64,2%). O menos freqüente Fusarium spp (A= 2,9%, B= 2,7% e M=1,5%). Na farinha de trigo integral o principal gênero encontrado foi o Penicillium spp e o Fusarium não foi isolado. O desoxinivalenol foi encontrado em uma amostra de farinha de trigo integral a uma concentração de 231 ng/g. O sistema APPCC se aplica aos moinhos e os auxilia a controlar suas produções do ponto de vista da segurança alimentar. / Food safety is a major concern facing the food industry today. Hazard Analysis Critical Control Point (HACCP) provides a structured approach to the assurance of safety of products. The aim of this study was to verify the use of HACCP system in a milling industry, precisely in the whole wheat flour production. At first step two hazards were analyzed: deoxynivalenol and allergenic mites in whole wheat flour Thirty (30) lots of flour were collected in an industry which capacity was 15 ton./h in São Paulo city. Those lots had 30 sub-samples of argentine (A) wheat (12,8% average moisture content), 17 sub-samples of brazilian (B) wheat (13,1% m.c.), 30 sub-samples of wheat for milling (M) (15,6% m.c), and 30 sub-samples of whole wheat flour (FTI) that were analyzed in a five months period (from 97/october to 98/march). Light filth was isolated from grains and flour, according to Official Methods of AOAC International. Methodology of extraction of light filth from whole wheat flour was studied to know the repeatability according to AOAC methods. Fungi isolation was performed according to AOAC/FDA Bacteriological Analytical Manual (1992) methodology; genera were identified according GELLI et al.(1990), SAMSON et al (1995), and PITI and HOCKING (1997). Detection and quantification of deoxynivalenol (DON) were done by thin layer chromatography (TLC) according to SABINO et al. (1989).The most significant light filth contamination in wheat were mites (A= 90%, B= 53%, M= 77% e FTI= 57%), argentine wheat had as a predominant mite the family Tarsonemidae and flour had Acaridae. The average fungal contamination in grains was 99%, 95% e 94% in argentine, brazilian and wheat for milling respectively. The predominant genera was Aspergillus spp ( A= 79,0, B=39,3% e M= 64,2 %), and Fusarium was the least frequent in wheat (A>/b>=2,7%, B= 2,8% e M=1,5%). Only one lot of whole wheat flour was contaminated by DON at level 231 ng/g. This study showed that HACCP system is appropriated for control hazards in milling industry. However, DON should be controlled before wheat get into industry, and this control need to be registered. Mites are new emergentes to cause allergy by ingestion and have to be controlled by GMP procedures.