Mitochondrial and supernatant glutamate-oxalacetate transaminases (EC 2.6.1.1) and supernatant glutamate-pyruvate transaminase (EC 2.6.1.2) were purified 89, 204 and 240-fold respectively, from dolphin muscle. Starch gel electrophoresis of crude and purified perparations revealed that all three enzymes exist as single forms. Km values of a-ketoglutarate, alanine, pyruvate and glutamate for the glutamate-pyruvate transaminase were 0.45, 8.2, 0.87 and 15 mM, respectively. For the glutamate-oxalacetate transaminases, the Km values of a-ketoglutarate, aspartate, oxalacetate and glutamate were 0.76, 0.50, 0.10 and 9.4 mM, respectively, for the mitochondrial form and 0.13, 2.4, 0.06 and 3.2 mM, respectively, for the supernatant form. In all cases, as the assay pH was decreased from pH 7.3, the Km values of the a-keto acids decreased while those of the amino acids increased. This caused the apparent equilibrium constants for the glutamate-oxalacetate transaminases to remain independent of pH. These values were 9.2 and 6.8 for the mitochondrial and supernatant forms, respectively where K'eq = [asPartate][α-ketoglutarate]/[glutamate][oxalacetate]. Studies of the inhibition of the glutamate-oxalacetate transaminases by dicarboxylic acids indicated that these enzymes may be controlled by pools of metabolic intermediates. Three key roles are suggested for the transminases in the energy metabolism of the diving mammal. First, it is believed that a combined action of the transaminases could enhance energy production during hypoxia by providing (1) fumarate from aspartate for the ATP producing reversal of succinate dehydrogenase and (2) α-ketoglutarate from glutamate for the GTP producing succinyl thiokinase reaction. Next, diving mammals probably accumulate more NADH than other mammals during hypoxia. The glutamate-oxalacetate transaminases seem particularly well suited for restoring redox balance via the malate-aspartate cycle after aerobic metabolism is resumed. Finally, since migrating divers oxidize large amounts of stored fats, the combined reactions of the transaminases could be instrumental in providing increased supplies of oxalacetate to condense with the fat derived acetyl CoA in the citrate synthase reaction. / Science, Faculty of / Zoology, Department of / Graduate

Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, 1977 / Vita. / Includes bibliographical references. / Ph. D. / Ph. D. Massachusetts Institute of Technology, Department of Chemistry

This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).

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Submitted by Irene Nascimento (irene.kessia@ufpe.br) on 2016-09-08T18:57:32Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) DISSERTAÇÃO - CLARISSA BARROS MADRUGA versão digital.pdf: 2723819 bytes, checksum: ae4a199db2f44ecf66614c6242c348b3 (MD5) / Made available in DSpace on 2016-09-08T18:57:32Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) DISSERTAÇÃO - CLARISSA BARROS MADRUGA versão digital.pdf: 2723819 bytes, checksum: ae4a199db2f44ecf66614c6242c348b3 (MD5) Previous issue date: 2015-06-18 / A dengue é importante causa de doença febril em países tropicais e de acordo com sua nova classificação a presença de sangramentos em mucosas e alterações hepáticas alertam para possível evolução desfavorável. O envolvimento do fígado manifesta-se principalmente por elevação de aminotransferases ou hepatomegalia e as formas hemorrágicas da dengue associam-se ao prolongamento precoce do TTPA. Foram selecionados 554 indivíduos com diagnóstico laboratorial de dengue a partir de banco de dados formado no período de março de 2009 a maio de 2011, divididos em dois grupos: com e sem sangramento espontâneo de mucosa, e os parâmetros laboratoriais (aminotransferases, TTPA e plaquetas) foram comparados entre eles. Dos 554 indivíduos, 285 (51,4%) eram do sexo feminino e 269 (48,6%) do sexo masculino, com idade média de 27,4 anos, mediana de 22 anos (3 meses a 92 anos), 219 (39,5%) tinham idade ≤ 15 anos e 335 (60,5%) com idade > 15 anos, 166 (30%) pacientes apresentaram manifestação hemorrágica espontânea, contra 388 (70%) que não apresentaram sangramento. Dentre os pacientes que sangraram, 81 (48,8%) eram do sexo masculino e 85 (51,2%) do sexo feminino, predominando na faixa etária ≤ 15 anos com 84/219 (38,4%) versus a faixa etária > 15 anos com 82/335 (24,5%). Pela localização, os sangramentos espontâneos apresentaram a seguinte ordem de ocorrência: gengivorragia, 30,3% (64/166); hematêmese, 26,5% (56/166); epistaxe, 22,3% (47/166); melena, 8,1% (17/166); hematúria, 6,6% (14/166), hemoptise; 3,3% (7/166) e hemotoquezia 2,8% (6/166). O prolongamento de TTPA foi encontrado em 23% (82/358) dos pacientes, enquanto 77% (276/358) tiveram TTPA normal. O prolongamento de TTPA foi mais frequente na faixa ≤ 15 anos com 51,2% versus a faixa etária >15 anos com 48,8% (p<0,05). Apenas 26% (138/524) apresentaram valores de AST maior que 175 e 74% (386/524) apresentaram AST até 175. Os valores de ALT apresentaram a seguinte distribuição: 16% (84/528) apresentam ALT maior que 200 e 84% (444/528) apresentam ALT até 200. Não houve associação entre elevação de aminotransferases e sangramentos (p > 0,05). Com a dicotomização das plaquetas em valores abaixo e acima de 50.000 mm3, o prolongamento de TTPA, apresentou resultado significativo para sangramentos espontâneos condicionado às plaquetas <50.000/mm³. Não foi encontrada associação entre alterações morfológicas do fígado e sangramentos espontâneos. Com a curva ROC foi estabelecido o ponto de corte de 41,3 segundos para a associação do TTPA com hemorragia espontânea de mucosa nos indivíduos estudados, com sensibilidade de 52,8%, especificidade de 69,2%, acurácia de 64,2%, Risco Relativo (RR) de 1,868 e Odds Ratio (OR) 2,511. Na análise multivariada apenas os fatores de risco prolongamento de TTPA e plaquetas apresentaram associação significativa quanto à ocorrência de sangramentos espontâneos (p < 0,05). O prolongamento de TTPA mostrou-se como possível preditor de sangramento, no entanto, faz-se necessário avaliação de outros elementos do coagulograma para que esse dado possa adquirir maior consistência. / Dengue is an important cause of febrile illness in tropical countries and according to the new classification the presence of mucosal bleeding and liver changes correlates with possible adverse developments. The involvement of the liver is manifested primarily by increased aminotransferases levels or hepatomegaly and hemorrhagic forms of dengue are associated with the early prolonged aPTT. Information from 554 patients with a laboratory diagnosis of dengue from the database formed from March 2009 to May 2011 were collected, divided into two groups: with and without spontaneous mucosa bleeding, and laboratorial parameters (aminotransferases, aPTT and platelet ) were compared. Of the 554 subjects, 285 (51.4%) were female and 269 (48.6%) were male, mean age 27.4 years, median 22 years (3 months to 92 years), 219 ( 39.5%) were aged ≤ 15 years and 335 (60.5%) aged> 15 years, 166 (30%) patients had spontaneous hemorrhagic manifestation, against 388 (70%) who did not have bleeding. Among the patients who bled, 81 (48.8%) were male and 85 (51.2%) were females, predominantly aged ≤ 15 years with 84/219 (38.4%) versus the age group > 15 years, with 82/335 (24.5%) (p <0.01). By location, spontaneous bleeding showed the following order of occurrence: gingival bleeding, 30.3% (64); hematemesis, 26.5% (56); epistaxis, 22.3% (47); melena, 8.1% (17); hematuria, 6.6% (14), hemoptysis; 3.3% (7) and hemotoquezia 2.8% (6). The extension of APTT was found in 23% (82/358) of patients, while 77% (276/358) had normal aPTT. The enlargement aPTT was more frequent in the range ≤15 years with 51.2% versus age> 15 years with 48.8% (p <0.05). Only 26% (138/524) had values of AST greater than 175 and 74% (386/524) had AST up to 175. ALT values were distributed as follows: 16% (84/528) have ALT greater than 200 and 84% (444/528) have ALT up to 200. There was no association between increased levels of aminotransferases and bleeding (p> 0.05). With the dichotomy of platelets in values below and above 50,000 mm3, the extension of aPTT, showed significant results (p <0.05) for spontaneous bleeding conditioning to platelets <50,000 / mm³. No association was found between morphological changes in the liver and spontaneous bleeding. With the ROC curve was established cutoff point of 41.3 seconds to the association of aPTT with spontaneous bleeding mucosa in studied subjects, with sensitivity 52.8%, specificity 69.2%, accuracy of 64.2% , relative risk (RR) of 1.868 and odds ratio (OR) 2.511. In multivariate analysis, only the risk factors enlargement aPTT and platelets were significantly associated for the occurrence of spontaneous bleeding (p <0.05). The extension of aPTT showed up as possible predictor of bleeding, however, it is necessary to evaluate other coagulation elements so that this data can acquire greater consistency.

Orientador: Jairo Osvaldo Cazetta / Banca: Antonio Álvaro Corsetti Purcino / Banca: Marcelo Murad Magalhães / Resumo: Embora esteja bem estabelecido pelos experimentos clássicos, qual são os fatores que limitam a produção, o funcionamento da planta na fase reprodutiva que envolve um complexo relacionamento tanto entre órgãos fonte e dreno de fotossintatos como do metabolismo do nitrogênio em ambos os tipos de órgãos, ainda permanece pouco esclarecido. Assim sendo, na fase de polinização foram impostas diferentes proporções de folhas (% de fonte) e de grãos (% de dreno) em plantas de milho para estudar o efeito destes tratamentos sobre o comportamento do metabolismo do nitrogênio em grãos, folhas e colmos, em diferentes etapas da fase reprodutiva da cultura e suas relações com a produção de massa seca, desenvolvimento de grãos, bem como desenvolvimento e senescência das folhas. Avaliou-se atividade de algumas enzimas, o teor dos principais metabólitos nitrogendados nas folhas, nos colmos e nos grãos em formação, bem como os reflexos destas variáveis sobre algumas características agronômicas aos 2, 10, 20 e 30 dias após a polinização (dap). Os resultados do presente trabalho permitiram esclarecer que a atividade da redutase do nitrato na folha não foi afetada pelas alterações nas proporções de fonte e dreno de fotossintatos. Os teores de N-total, N-nitrato e N-aminoácidos livres, nas folhas, colmos e endospermas foram mais intensamente afetados quanto mais drásticas foram as reduções de folhas ou grãos. As reduções da fonte e dreno promoveram aumentos significativos nos teores de N-total, N-nitrato e N-aminoácidos livres nas partes remanescentes analisadas. Os teores de proteína solúvel foram mais afetados nos grãos, onde os maiores valores foram encontrados aos 10 dap., nos tratamentos sem folhas e sem grãos... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Although it is well very established, for the classic experiments, which are the factors that limit the production, the operation of the plant in the reproductive phase that involves a compound so much relationship between organs source and fotossintatos drain as of the metabolism of the nitrogen in both types of organs, it remains unclear. Like this being, in the pollination phase different proportions of leaves were imposed (% of source) and of grains (% of drain) in corn plants to study the effect of these treatments on the behavior of nitrogen metabolism in grains, leaves and stems, in different stages during reproductive phase of the culture and your relationships with the production of dry mass, development of grains, as well as development and senescence of leaves. Enzymes activity were evaluated (NR, TGO and TGP), the level of main metabolites (N-total, N-nitrate, free amino acids and soluble protein) in the leaves, in the stems and in the grains in formation, as well as the reflexes of these varied on the agronomic characteristics (mass evaporates of leaves stems and grains), to the 2, 10, 20 and 30 days after the pollination (dap). The results of the present work allowed to clear that the activity of the nitrate reductase in the leaf was not affected by the alterations in the source proportions and photoassimilated drain. The levels of N-total, N-nitrate and free N-amino acids, in the leaves, stems and endosperms were more intensely affected the more drastic they were the reductions of leaves or grains. The reductions of the source and drain promoted significant increases in the levels of N-total, N-nitrate and free N-amino acids in the analyzed remaining parts. The soluble protein concentration was more affected in the grains, where the largest values were found to the 10 dap, in the treatments without leaves and grains... (Complete abstract, click eletronic address below). / Mestre

In this study, the possible relationship between aminotransferase activities and markers of oxidative stress in hepatitis C patients was evaluated. Patients with HCV (hepatitis C virus) infection confirmed by positive HCV RNA in serum, without treatment to hepatitis C were divided into three groups: group I (15 to 39 U/L); group II (41 to 76 U/L); group III (81 to 311 U/L) of alanine aminotransferase (ALT) activity. A number of parameters were examined in blood as indicators of oxidative stress, including catalase, gluthatione peroxidase, thiobarbituric acid-reactive species (TBARS), non-protein thiol groups (NP-SH), protein thiol groups (P-SH) and vitamin C. The results demonstrated that markers of oxidative stress NP-SH, P-SH, TBARS, glutathione peroxidase and catalase activities measured in blood of these study groups were not significantly differents (P>0.05). The antioxidant vitamin C was significantly decreased in Group III (P=0.001) and Group II (P=0.03) when compared with Group I. The vitamin C level correlated negatively with AST activity (r=-0.29, P=0.042) for all patients. Our data suggested that vitamin C in plasma determination could be an additional indicator of hepatitis C severity, since plasma vitamin C was negatively associated with aminotransferase activities. Antioxidant therapy, such as vitamin C, may therefore have a role in retarded disease progression in liver / Neste trabalho, a possível relação entre a atividade das aminotransferases e marcadores de estresse oxidativo em pacientes com hepatite C foi avaliada. Pacientes com infecção pelo vírus da hepatite C (HCV) confirmada por HCV RNA positivo no soro, sem tratamento, foram divididos em três grupos: grupo I (15 a 39 U/L); grupo II (41 a 76 U/L); grupo III (81 a 311 U/L) de atividade da alanina aminotransferase (ALT). Um número de parâmetros foi examinado no sangue como indicadores de estresse oxidativo, incluindo catalase, glutationa peroxidase, espécies reativas ao ácido tiobarbitúrico (TBARS), grupos tióis protéicos (P-SH), grupos tióis não protéicos (NP-SH) e vitamina C. Os resultados demonstraram que os marcadores P-SH, NP-SH, TBARS, atividade da glutationa peroxidase e catalase dosadas no sangue dos indivíduos incluídos nos grupos de estudo não foram diferentes significativamente (P>0.05). O antioxidante vitamina C foi significativamente diminuído no grupo III (P=0.001) e no grupo II (P=0.03) quando comparado com o grupo I. A vitamina C correlacionou-se negativamente com atividade da AST (r=-0.29; P=0.042) em todos os grupos. Nossos dados sugerem que a dosagem da vitamina C no plasma pode ser usada como um indicador adicional da severidade da hepatite C, já que foi negativamente associada com a atividade das aminotransferases. A terapia com antioxidantes, assim como vitamina C, pode, então, ter um papel no retardo da progressão da doença hepática

Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / In the present work, behavioral, neurochemical (determination of monoamines and metabolites levels rat in striatum), histopatological and biochemical (lipoproteins and transaminases) alterations produced by cocaine, ethanol and the association of theses were analyzed. Females Wistar rats (180-200 g) were treated during 7 days with cocaine (Coc 10 and 20 mg/kg, i.p.), ethanol (Et 2 and 4g/kg, p.o.) and the association of theses (Coc 10 mg + Et 2g - low interaction doses; Coc 20 mg + Ethanol 4g - high interaction doses). The results demonstrated that the spontaneous locomotor activity (SLA) was increased after cocaine administration and decreased after ethanol in both doses. It was not observed alterations in the SLA in the association cocaine + ethanol. The treatment with cocaine and ethanol caused an increase in dopamine level. The association cocaine + ethanol in higher doses caused an increase of dopamine and serotonin and decrease of DOPAC levels, suggesting that those drugs would can actuate directly in those systems or, indirectly, across a process of modulation. Cocaine, ethanol and the association of theses, after subcronic administration and in both doses, caused a donwregulation of D2-like receptors, not by recurring alterations in the values of Kd. The values of Bmax and Kd of the M1 + M2-like receptors have not already suffered alterations. In the biochemical study, the administration of cocaine induced an increase the concentrations of TGO and triglycerides, and decrease of the concentrations of TGP, total cholesterol and HDL. The treatment with ethanol decreases the levels of HDL, total cholesterol and triglycerides. The association cocaine + ethanol caused in both doses decrease of triglycerides, HDL, TGP and total cholesterol. All treatments did promote histopatological alterations in cardiac and hepatic woven. Ours results suggest that the association cocaine + ethanol appears to interfere more intense in the systems of neurotransmitters and in the biochemical parameters than the use of the isolated drugs. / No presente trabalho foram estudadas as alteraÃÃes comportamentais, neuroquÃmicas (determinaÃÃes dos nÃveis de monoaminas e metabÃlitos), histopatolÃgicas e bioquÃmicas (lipoproteÃnas e transaminases) em corpo estriado de ratos tratados com cocaÃna e etanol isoladamente ou em associaÃÃo. Foram utilizadas ratas Wistar (180-200 g), que foram tratadas durante 7 dias com cocaÃna (Coc 10 e 20 mg/kg, i.p.), etanol (Et 2 e 4g/kg, v.o.) e a associaÃÃo destes (Coc 10 mg + Et 2g - interaÃÃo baixas doses; Coc 20 mg + Etanol 4g - interaÃÃo altas doses). Os resultados demonstraram que a atividade locomotora espontÃnea (ALE) foi aumentada apÃs administraÃÃo de cocaÃna em ambas as doses e diminuÃda apÃs a administraÃÃo de etanol em ambas as doses. NÃo foram observadas alteraÃÃes na ALE na associaÃÃo cocaÃna + etanol. O tratamento com cocaÃna e etanol causou um aumento de dopamina, sem alteraÃÃes nos demais neurotransmissores e metabÃlitos. A associaÃÃo cocaÃna + etanol em altas doses, promoveu aumento dos nÃveis de dopamina, diminuiÃÃo de DOPAC e aumento dos nÃveis de 5-HT, sugerindo que essas drogas poderiam atuar diretamente nesses sistemas ou, indiretamente, atravÃs de um processo de modulaÃÃo. A cocaÃna, etanol e a associaÃÃo destes, apÃs administraÃÃo sub-crÃnica e em ambas as doses, causou uma downregulation em receptores D2-sÃmile, nÃo ocorrendo alteraÃÃes nos valores de Kd. Os valores de Bmax e Kd dos receptores M1 + M2-sÃmile nÃo sofreram alteraÃÃes. No estudo bioquÃmico, a administraÃÃo de cocaÃna induziu um aumento nas concentraÃÃes de TGO e triglicerÃdeos, e diminuiÃÃo das concentraÃÃes de TGP, colesterol total e HDL. O tratamento com etanol diminuiu os nÃveis de HDL, colesterol total e triglicerÃdeos. A associaÃÃo cocaÃna + etanol promoveu em ambas as doses diminuiÃÃo de trigicerÃdeos, HDL, TGP e colesterol total. Todos os tratamentos promoveram alteraÃÃes histopatolÃgicas em tecido cardÃaco e hepÃtico. Nossos resultados sugerem que a associaÃÃo cocaÃna + etanol parece interferir de maneira mais intensa nos sistemas de neurotransmissÃo e nos parÃmetros bioquÃmicos do que o uso das drogas isoladas.

The concerns of the present study were to determine 1) the conditions necessary to measure displaceable [³H] L-aspartate binding to membrane fractions of the rat brain, 2) whether the binding demonstrated the charcteristics of the site which is active in vivo, and 3) whether the acidic amino acid neurotransmitters aspartate and glutamate bind to identical or different sites by comparing the pharmacological specificities of the [³H] L-aspartate binding with that of [³H] L-glutamate. The conditions of the [³H] L-aspartate binding assay were determined in synaptosomal and total particulate fractions of whole rat brain. The reaction mixture which included the membrane fraction suspended in Tris-HCl buffer (pH 7.4) in the presence or absence of the compound under test, was incubated at 37°C for 30 minutes. The reaction was stopped by centrifugation and the radioactivity in the pellet counted by liquid scintillation spectrometry. The [³H] L-aspartate binding was characterized in total particulate fractions of rat cerebellum. The apparent dissociation constant (K[sub=D]) and maximum binding (Bmax), as determined by Scatchard analysis, are 1.64 ± 0.34 μM and 7711 ± fmol/mg protein respectively. The displaceable binding is reversible, saturable, independent of the presence of NA⁺, has an affinity in the range where the neurotransmitter is active in vivo, and demonstrates a pharmacological specificity which includes stereospecificity. The compounds tested to demonstrate the pharmacological specificity were L-aspartate (IC[sub=50] = 1.81 μM), D-aspartate (IC[sub=50] = 46.6 μM), L-glutamate (IC[sub=50] = 1.24 μM), N-methyl-DL-aspartate (inactive), kainate (inactive), D-alpha-aminoadipate (inactive), and L-alpha-aminoadipate (IC[sub=50] =7.12 μM). The pharmacological specificity of [³H] L-aspartate binding was different from that of [³H] L-glutamate. When the binding data only are considered, therefore, separate receptors for aspartate and glutamate are indicated. The pharmacological specificity of the [³H] L-aspartate binding, that is the affinity of the binding site for N-methyl-DL-aspartate, D- and L-alpha-aminoadipate, however, does not correlate with the potency of these compounds derived from iontophoretic studies. L-alpha-aminoadipate is very effective while N-methyl-DL-aspartate and D-alpha-aminoadipate do not displace the [³H] L-aspartate binding. In iontophoretic studies, N-methyl-D-aspartate and D-alpha-aminoadipate are very potent as compared to aspartate while L-alpha-aminoadipate Is inactive. The [³H] L-aspartate binding then may not represent the site which is active in vivo. The characteristics of the aspartate site in vivo, however, may not be truely represented in iontophoretic studies because of, for example, uptake of the compounds. The aspartate binding site, therefore, must be identified as that which is activated in vivo. The question of separate receptors for aspartate and glutamate then must still be resolved. / Science, Faculty of / Zoology, Department of / Graduate

Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2014-11-07T10:55:53Z No. of bitstreams: 2 Dissertação - Mirian de Sylvio - 2009.pdf: 865608 bytes, checksum: bba24a89ef1938c31376520a3eff1e60 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2014-11-07T11:29:38Z (GMT) No. of bitstreams: 2 Dissertação - Mirian de Sylvio - 2009.pdf: 865608 bytes, checksum: bba24a89ef1938c31376520a3eff1e60 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2014-11-07T11:29:38Z (GMT). No. of bitstreams: 2 Dissertação - Mirian de Sylvio - 2009.pdf: 865608 bytes, checksum: bba24a89ef1938c31376520a3eff1e60 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2009-12-15 / Infection with Toxoplasma gondii occurs throughout the globe, with a prevalence of up to 90% in the population. The physiological changes caused by this parasite are well studied in immunocompromised individuals and in cases of congenital transmission. In immunocompetent individuals the infection is usually asymptomatic and little explored by researchers. Experimental studies follow the pattern of human studies, and there fow mention about the biochemical changes (liver and kidney metabolisms) in the host infected by T. gondii. This study aimed the quantification of hepatic and kidney alterations caused by acute infections by T. gondii (non cystogenic strain – RH) and by chronic infections (cystogenic strain – ME-49). The control group was formed by mice without infection, only submitted to saline stress. Several enzymes were measured in serum and peritoneal exudate of mice infected and control such as: aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutamyltransferase (GGT), alkaline phosphatase (ALP), urea, creatinine and lactate dehydrogenase, using an automated methodology. AST and ALT presented a significative difference in the serum of mice infected with RH strain when compared to controls indicating a destruction of liver cells. The peritoneal exudates did not present significative changes in relation to controls nor did the urea and creatinine levels. The séric lactate dehydrogenase showed gradual changes in all days of the infection in mice peritoneal exudates as early as this change was evident only in the fifth day of infection. All samples of the group infected with ME-49 strain showed changes in serum and peritoneal exudate during all days of analysis. Only ALT peritoneal exudates showed no change during all days of analysis. An increase in urea at all doses was observed, however, creatinine showed a change only within 120 days of infection. The LDH was altered in the serum in all days of analysis. In conclusion, the T. gondii infection may cause hepatic and kidney injuries either when caused by non-cystogenic as by cystogenic strains of the parasite. / A infecção pelo Toxoplasma gondii ocorre em todo o mundo, com prevalência de até 90% na população conforme seus hábitos culturais e condições socioeconômicas. As alterações fisiopatológicas provocadas por este parasito são muito estudadas nos indivíduos imunocomprometidos, nos casos de transmissão congênita, e nos indivíduos imunocompetentes a infecção é, geralmente, assintomática e pouco explorada pelos pesquisadores. Experimentalmente, os estudos seguem o padrão dos estudos humanos, e há pouca referência sobre as alterações bioquímicas (hepáticas e renais) no hospedeiro infectado pelo T. gondii. Este trabalho objetivou avaliar as alterações hepáticas e renais causadas por esse parasito em camundongos na fase aguda, usando a cepa não cistogênica (RH), e na fase crônica, com a cepa cistogênica (ME-49), tendo como controles camundongos sem infecção, somente submetidos ao estresse de inoculação com salina. Foram dosadas no soro e no exsudato peritoneal dos camundongos infectados e controles os níveis das enzimas Aspartato aminotransferase (AST), Alanina aminotransferase (ALT), Gamaglutamiltransferase (GGT), Fosfatase alcalina (FAL), desidrogenase lática (DHL) e dos seguintes compostos: uréia e creatinina, por metodologia automatizada. As enzimas AST e ALT apresentaram diferença significativa no soro de camundongos infectados com cepa RH, demonstraram alterações em relação aos controles indicando uma destruição das células hepáticas. No exsudato peritoneal não foram demonstradas alterações em relação aos controles. A uréia e creatinina dosadas não demonstraram alteração significativa. A enzima lactato desidrogenase sérica apresentou alterações gradativas em todos os dias de infecção do camundongo no soro, já no exsudato peritoneal essa alteração foi evidenciada somente no quinto dia da infecção, mostrando que com o aumento de parasitos e a destruição celular causada por esse, essa enzima presente em várias células é responsável por demonstrar aumentos consideráveis. Todas as amostras de soro analisadas do grupo infectado com a cepa ME-49 demonstraram alterações durante todo período de acompanhamento. Enquanto que no exsudato peritoneal não mostrou nenhuma alteração durante todo período analisado. Houve aumento crescente na uréia em todos os dias de analises, porém, a creatinina não apresentou nenhuma alteração. A LDH mostrou-se alterada no soro em todos os dias de analisado. Conclui-se que a infecção pelo T. gondii pode provocar alterações hepáticas e renais ao longo do curso de infecção, tanto em infecções com cepa cistogênica quanto com cepa não cistogênica.