Graduation date: 1997

Comme le nucléole joue un rôle fondamental dans l’expression des protéines, via la synthèse des ARN ribosomiques, il n’est donc pas surprenant que des études aient révélé un lien étroit, entre des dysfonctionnements nucléolaires et l’origine de certaines maladies humaines. La découverte, il y a plusieurs années, d’un taux anormalement élevé de protéines nucléolaires dites argyrophiles ou AgNORs, dans les cellules tumorales, a permis d’envisager leur utilisation comme outil diagnostique ou pronostique du cancer. Détectées, de manière in vitro grâce à leur affinité pour l’argent, l’identification de quelques protéines AgNORs n’a pourtant pas permis d’établir une caractéristique commune à toutes les protéines argyrophiles détectées dans les extraits nucléolaires. Ainsi, bien que le test colorimétrique AgNOR soit utilisé dans de nombreux laboratoires académiques, l’absence d’identification de protéines AgNORs spécifiques du processus de cancérisation, a limité son utilisation en laboratoire clinique. Comme certaines limites technologiques et expérimentales ont limité leur caractérisation chez l’humain, nous avons donc décidé de reprendre les recherches sur ce sujet et de le réactualiser grâce aux avancées technologiques et scientifiques. Les protéines AgNORs étant étroitement liées à la biogenèse des ribosomes, nous avons donc décidé d’amorcer nos recherches chez la levure Saccharomyces cerevisiae, dans laquelle, la voie de biosynthèse des ribosomes a été particulièrement bien décrite. Devant l’intérêt biologique et médical de ces protéines, l’objectif de ce projet a donc été triple : 1-identifier des protéines AgNORs chez la levure 2-caractériser les propriétés physico-fonctionnelles et physico-chimiques de ces protéines AgNORs. 3-utiliser ces caractéristiques physico-chimiques pour rechercher de nouvelles AgNORs humaines, spécifiques de processus de cancérisation et potentiellement utilisables comme marqueurs tumoraux./The nucleolus is a subnuclear compartment that organized around ribosomal gene (rDNA) repeats NORs, which encode for ribosomal RNA. A peculiar group of acidic proteins which are highly argyrophilic are also localized at the same sites as NORs, thus allowing NORs to be very clearly and rapidly visualized by silver nitrate staining procedures. However, if three human argyrophilic proteins, UBF, C23 (nucleolin) and B23 (nucleophosmin), have been associated for staining of NOR, the exact number of AgNOR proteins and their intrinsic biochemical feature are unclear. Here, we have performed an heterologous screen in a genetically tractable eukaryotic organism (budding yeast) for the identification of novel AgNOR proteins and in vitro characterized an intrinsic feature that underlies silver binding and offers a strong predictive value for the identification of novel human AgNOR proteins.

<p>The question this paper seeks to answer is whether decentralization is helpful or harmful to Cameroon&rsquo / s national unity. This study traces the historical, constitutional and political development of the concepts of national unity and decentralization and critically examines their application in the Cameroonian context. It further tests the consolidation of national unity in Cameroon against a theoretical and empirical framework of decentralization. A one-dimensional view of the findings of this study is not very encouraging to regimes seeking to enhance national unity through the implementation of decentralization. However, the major conclusion of this study holds the position that the political outcome of decentralization on Cameroon&rsquo / s national unity is largely a product of the constitutional regulation of both concepts and the manner in which the theoretical dimensions of decentralization are transplanted onto Cameroon&rsquo / s political landscape. The paper recommends certain reforms to assist and guide Cameroon as it simultaneously implements decentralization and consolidates national unity.</p>

The purpose of this thesis is to disentangle the causes behind differences in regional employment across the 81 Swedish LA regions. Thus, two questions will be answered; which factors causes regional disparity in employment and which where the least and the most affected regions during the economic crises of the 1990’s? The answer to these questions are imposed by certain chosen restrictions, where only the situation within two manufacturing industries will be investigated; the car- and machine manufacturing sectors. Previous research claim that there are specific factors that influence and creates regional growth disparity. Among these factors can be found; education, infrastructure, demography, industry diversity and migration. Statistical data then enables a division of the regions on basis of the change in employment level within the manufacturing industries as a share of total employment. It is revealed that the most affected regions during an economic shock are those areas that have the highest employment ratio within these manufacturing sectors. The empirical findings indicates that in the case of Swedish manufacturing industries especially three factors influence the employment level; population, education and migration. Additionally, distance to a larger city is proven to be significant during recessions while being insignificant during economic booms. The last factor, diversity, on the other hand indicates that the correlation is the reverse. Hence, diversity has an impact during economic upswings, while this is not the case during downturns. With the development during the 90’s as a reference, the same method is used to locate today’s most vulnerable industrial regions. Statistics show that Ljungby is at the top of this list. When studying the strategic development plan for this region it is found that this area follows a policy in line with those variables that this thesis has pin-pointed to be beneficial for regional growth. Hence, this region has taken beneficial policy steps in order to decrease the dependency on a vulnerable and market sensitive industrial sector. / Syftet med denna uppsats är att utreda varför anställningsgraden skiljer sig mellan de 81 svenska LA regionerna. De två frågor som skall besvaras är följaktligen; vilka faktorer påverkar skillnader i sysselsättningsgrad samt utpeka vilka regioner var de minst och mest drabbade under 1990-talets ekonomiska kris? Dessa frågor har dock begränsats till att undersöka förhållandet inom två tillverkningsindustrier; bil- och maskintillverkning. Tidigare forskning hävdar att det finns vissa specifika faktorer som påverkar och skapar regionala skillnader i tillväxt. Bland dessa kan nämnas utbildning, infrastruktur, demografi, diversitet och migration. Den deskriptiva statistiken delar därefter upp Sveriges regioner på basis av förändringen i anställning inom de två valda industrisektorerna som en del av totala sysselsättningen. De hårdast drabbade regionerna under en lågkonjunktur är deregioner som har störst andel av totala arbetskraften inom tillverkningsindustrin. Vidare indikerar de empiriska resultaten att för svensk tillverkningsindustris vidkommande så har främst population, utbildning samt migration ett starkt samband med sysselsättningsfrekvensen. Vidare visar det sig att avståndet till en större stad har en inverkan under lågkonjunktur men ej under högkonjunktur. För den sista variabeln, grad av diversitet, visar sig sambandet vara det motsatta; det vill säga ingen påverkan under en konjunkturnedgång, medan en positiv influens under konjunkturuppgång. Med händelseutvecklingen från 90-talet som grund kan samma metod användas för att lokalisera dagens mest sårbara industriregioner. Statistiken visar att Ljungby toppar denna lista. Men då man studerar denna regions framtids- och utvecklingsmål följer dessa just de faktorer som denna uppsats utpekar som viktiga ingredienser för regional tillväxt. Följaktligen har denna region tagit positiva steg i riktning mot att minska sitt beroende av en sårbar och konjunkturkänslig industrisektor.

This is unique study of import is of the greatest importance, since no studies of import across regions have earlier been performed. Import is a driving force of innovations and therefore most important for a stable growth. This master thesis is about interregional import, as well as the strong spatial concentration of imports in the Swedish system of network. Five hypothesises are presented in the last section of chapter two. The variables to be used in the analysis are then divided into two main groups, before empirically tested in different combinations of regression models. The main conclusion of this thesis is a significant correlation between import, and the two independent variables export and firm R&amp;D, and its result goes in line with the theoretical framework of this thesis, regional specialisation in import and export nodes

During the last decades a stronger role for the regions has developed in many West European countries. To a significant degree this regionalisation trend has coincided with European integration. The key change in the role of the regional level has been with regard to its status as an agent of regional development. In most West European countries there has been a shift from an approach to regional policy that has focused on redistributive measures from the centre in which the regions play a relatively weak role to a perspective that is sometimes labelled “new regionalism” in which the focus is on the region taking responsibility for its own development. In this new regionalist perspective, which is both descriptive and normative, the region is considered as the appropriate arena for both economic activities and decision-making. In the political systems of the Nordic countries the regional level has traditionally been in a relatively weak position and regional policy has emphasized centralisation and redistributive measures. Not unexpectedly, the pan-European trend toward a stronger role for the region has also found its way to the Nordic countries. The aim of this study is to describe and analyze if and to what extent key actors in three peripheral regions, situated in countries with a strong tradition of redistribution from the centre and a weak role for the regional level, have embraced the new regionalist perspective. The three regions are Troms in Norway, Pohjois-Pohjanmaa in Finland and Västerbotten in Sweden. All are peripherally located with small populations and economies that rely heavily on natural resources. The analysis is based on interviews with regional and local politicians, civil servants, and business representatives. The empirical material is presented in the form of narratives formulated by the regional actors who express their views on regional policy and the role of the region. The results of the study show that regional actors in the three peripheries express similar narratives. To a certain degree actors have embraced the new regionalist perspective in the sense that they see the regional level as an important coordinator for development initiatives and measures. However, the actors’ claims for a stronger regional level must be understood in the context of the unitary state. In this context, the actors’ perspective combines the new regionalist and the centralist redistributive approach, one that can be labelled ‘redistributive regionalism’. The state remains the key actor and is expected to guarantee equal conditions in all parts of the country. The emphasis on strengthening the administrative region is more pronounced in Troms and Västerbotten than in Pohjois-Pohjanmaa, where instead there is a stronger focus on the functional region. Actors in the three regions do not see any contradiction between a strong state and increased regional influence on development issues. In sum, the study finds that the new regionalist perspective has been embraced to a certain extent but that it has been adapted to national characteristics, as well as to the specific conditions in the three regions.

Regionalism is a concept of extensive proportions and as such is complex to define. This phenomenon becomes much clearer by studying it from different perspectives. This paper focuses on regional political parties and description of their political ideas. The goal of this paper is to examine and compare regional political parties in two Swedish regions: Norrbotten and Skåne. By studying two biggest regional parties in these regions, namely Norrbottensparti and Skånepartiet, I will try to get an insight into the regional politics, as well as illustrate regionalism and its meaning. My primary intention is to examine how these two regional parties experience their regions and what they consider of importance in regional development. By studying these regional parties and their opinion/apprehension about regional politics and their ideological/political affiliation, I will try to illustrate an ideological dimension of regionalism. My intention is to point out differences and similarities between politics of these regional parties, as well as to illustrate approach to the regionalism and its meaning.

<p>Governing an ethnically diverse country constitutes a major challenge for state power and government in Cameroon. The call for national unity, championed by the regime in power has had to survive strong demands for greater autonomy and threats of secession by groups from within an English-speaking minority. In response to these demands and threats, and in conjunction with reforms to improve democratic governance and service delivery, Cameroon&rsquo / s state administration has in the last decade resorted to decentralization as a technique for promoting national unity. The question this paper seeks to answer is whether decentralization is helpful or harmful to Cameroon&rsquo / s national unity. This study traces the historical, constitutional and political development of the concepts of national unity and decentralization and critically examines their application in the Cameroonian context. It further tests the consolidation of national unity in Cameroon against a theoretical and empirical framework of decentralization. A one-dimensional view of the findings of this study is not very encouraging to regimes seeking to enhance national unity through the implementation of decentralization. However, the major conclusion of this study holds the position that the political outcome of decentralization on Cameroon&rsquo / s national unity is largely a product of the constitutional regulation of both concepts and the manner in which the theoretical dimensions of decentralization are transplanted onto Cameroon&rsquo / s political landscape. The paper recommends certain reforms to assist and guide Cameroon as it&nbsp / simultaneously implements decentralization and consolidates national unity.</p>

Hydrological models have been developed to estimate snow accumulation, snowmelt and snowmelt runoff on the Canadian Prairies; however, their proper scale of application is unknown in the Prairie environment. The first objective of this thesis is to examine the proper scale for pre-melt snow accumulation as snow water equivalent (SWE) and snowmelt in a Prairie first order basin. Spatially distributed and spatially aggregated approaches were used to calculate SWE and snowmelt at St. Denis National Wildlife Area (SDNWA). Both approaches used models with similar physics, but differed in the model scale at which calculations were carried out. The simulated pre-melt SWE, cumulative seasonal SWE, and daily snowmelt from the two modelling approaches were compared to field observations of pre-melt SWE, cumulative seasonal SWE, and daily snowmelt; comparisons of areal cumulative seasonal SWE, areal snowmelt, snowmelt duration, and snow-covered area were also conducted between two modelling approaches. Results from these comparisons showed that both approaches had reasonable and similar accuracy in estimation of SWE and snowmelt. The spatially aggregated approach was more computationally efficient and was selected as a modelling scale for small-sized prairie basins. <p>Another objective of this thesis is to derive a snow hydrology model for the Canadian Prairies. Physically-based hydrological models were assembled in the Cold Regions Hydrological Model Platform (CRHM) using the aggregated approach. Tests of pre-melt SWE and surface snowmelt runoff were conducted at two basins in Saskatchewan Creighton Tributary of Bad Lake and Wetland 109, St. Denis. Results showed that the snow hydrology model had a reasonable capability to simulate SWE and snowmelt runoff to the stream and wetland. <p>Droughts are natural hazards that develop frequently on the Canadian Prairies. Analyzing the impact of drought on hydrological processes and water supply is another objective of this thesis. Synthetic drought scenarios were proposed for the Creighton Tributary of Bad Lake and the corresponding impacts on the snowmelt runoff-related processes were examined. Results indicated that wind redistribution of snow was very sensitive to drought conditions, sublimation of blowing snow and snow-covered period were sensitive to drought, but winter evaporation and infiltration did not show strong trend. The results also showed that drought conditions had magnified effects on the snowmelt runoff and could cause cessation of streamflow. Also, the impacts of the recent 1999-2005 drought on the snowmelt hydrology were investigated at St. Denis. Results illustrated that three-years (1999-2002) of severe winter drought were followed by a normal year (2002-03) and then a two-year (2003-05) recovery period, and then returning to normal (2005-06). Results showed that both snowfall and rainfall during hydrological winter were consistently low for severe drought and surface snowmelt runoff was very much lower during severe drought, about 45-65 mm less compared to that in the normal periods.

<p>The cell cycle is one of the fundamental processes in all living organisms, and all cells arise from the division of existing cells. To better understand the regulation of the cell cycle, synchrony experiments are widely used to monitor cellular dynamics during this process. In such experiments, a large population of cells is generally arrested or selected at one stage of the cycle, and then released to progress through subsequent division stages. Measurements are then taken in this population at a variety of time points after release to provide insight into the dynamics of the cell cycle. However, due to cell-to-cell variability and asymmetric cell division, cells in a synchronized population lose synchrony over time. As a result, the time-series measurements from the synchronized cell populations do not accurately reflect the underlying dynamics of cell-cycle processes.</p><p>In this thesis, we introduce a deconvolution algorithm that learns a more accurate view of cell-cycle dynamics, free from the convolution effects associated with imperfect cell synchronization. Through wavelet-basis regularization, our method sharpens signal without sharpening noise, and can remarkably increase both the dynamic range and the temporal resolution of time-series data. Though it can be applied to any such data, we demonstrate the utility of our method by applying it to a recent cell-cycle transcription time course in the eukaryote <italic>Saccharomyces cerevisiae</italic>. We show that our method more sensitively detects cell-cycle-regulated transcription, and reveals subtle timing differences that are masked in the original population measurements. Our algorithm also explicitly learns distinct transcription programs for both mother and daughter cells, enabling us to identify 82 genes transcribed almost entirely in the early G1 in a daughter-specific manner.</p><p>In addition to the cell-cycle deconvolution algorithm, we introduce <italic>DOMAIN</italic>, a protein-protein interaction (PPI) network alignment method, which employs a novel <italic>direct-edge-alignment</italic> paradigm to detect conserved functional modules (e.g., protein complexes, molecular pathways) from pairwise PPI networks. By applying our approach to detect protein complexes conserved in yeast-fly and yeast-worm PPI networks, we show that our approach outperforms two widely used approaches in most alignment performance metrics. We also show that our approach enables us to identify conserved cell-cycle-related functional modules across yeast-fly PPI networks.</p> / Dissertation