Made available in DSpace on 2014-06-11T19:23:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-07-28Bitstream added on 2014-06-13T19:29:24Z : No. of bitstreams: 1 aragon_cc_me_arafcf.pdf: 397051 bytes, checksum: e29ac6a4f5baf629041e6e9442044f9e (MD5) / Universidade Estadual Paulista (UNESP) / No presente trabalho, a atividade da enzima glicerol quinase (GK; EC 2.7.1.30; ATP: glicerol 3-fosfotransferase), proveniente de extratos de levedura seca de panificação, foi otimizada. A melhor preparação enzimática da GK foi obtida por rompimento celular com esferas de vidro, durante sete minutos, com lise de 54,2% das células. O extrato celular foi parcialmente purificado com 1% de sulfato de estreptomicina, antes da precipitação com igual volume de solução a 30% (m/v) de polietilenoglicol 3350, e posteriormente dialisado. A atividade máxima da GK foi obtida em pH 10,0, a 60ºC e 50mM de substrato, por metodologia clássica. A enzima apresentou alta estabilidade térmica ― a atividade foi completamente mantida até 50ºC, durante uma hora ― e em pH entre 6,0 e 8,0. Além disso, manteve-se estável, por quatro meses, a 4°C, na presença de azida de sódio 0,05% e cloreto de cobalto 10mM, e, por até oito meses, com o extrato liofilizado. Calculados pelos métodos de Lineweaver-Burk, Hanes-Woolf e Eadie-Hofstee, o valor da constante de Michaelis (Km) da enzima variou entre 1,99mM e 3,11mM, e a Vmax, entre 1,14U/mL e 1,19U/mL. Utilizou-se a metodologia de superfície de resposta (MSR) para melhor definição dos parâmetros da reação enzimática, observando-se valores ótimos de atividades a temperaturas entre 52ºC e 56ºC, pH entre 10,2 e 10,5 e concentração de substrato de 150mM a 170mM. A MSR mostrou-se adequada para modelar a reação e maximizar a atividade da glicerol quinase. Este método, de baixo custo, dosa a glicerol quinase em uma seqüência de reações, sendo de grande importância para diversas indústrias, como a de alimentos, açúcar e álcool. / In the present study, the activity of the enzyme glycerol kinase (GK; EC 2.7.1.30; ATP: glycerol 3-phosphotransferase) from dry baker´s yeast, was optimized. The best enzymatic preparation of GK was obtained by cell disruption with glass beads, for seven minutes, with 54.2% of lysed cells. Cell extract was partially purified with 1% of streptomycin sulphate, before the precipitation with equal volume of a 30% solution (m/v) of polyethylene glycol 3350, and then it was dialyzed. The maximum activity of GK was obtained with pH 10.0, at 60ºC and 50mM of substrate, by the classic methodology. The enzyme presented high thermal stability ― the activity was completely maintained up to 50ºC, during one hour ― and at pH between 6.0 and 8.0. Besides, it was stable, for four months, at 4°C, in the presence of sodium azide 0.05% and cobalt chloride 10mM, and, for up to eight months, with the lyophilized extract. The value of the Michaelis constant (Km) of the enzyme was calculated by the methods of Lineweaver-Burk, Hanes-Woolf and Eadie-Hofstee,and it varied between 1.99mM and 3.11mM, and Vmax, between 1.14U/mL and 1.19U/mL. Response surface methodology (RSM) was used for better definition of the parameters of the enzymatic reaction, being observed higher activity values at temperatures between 52ºC and 56ºC, pH between 10.2 and 10.5 and substrate concentration from 150mM to 170mM. RSM showed to be an adequate approach for modeling the reaction and maximizing the glycerol kinase activity. This low cost method doses glycerol kinase in a sequence of reactions, being of great importance for many industries, like food, sugar and alcohol.

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<p>Bioethanol is a fuel of tomorrow, and progress in the use of enzymes and reduction of non-fermentable materials by debranning will probably be a part to make it more economical with low environmental impact.</p><p> </p><p>Ethanol production based on debranned wheat was optimized in this study by batch experiments as well as continuous experiments in laboratory scale. Enzymes from Novozymes and Genencor were compared and no significant differences were discovered between the different set of enzymes. The yeast strains Ethanol Red and AmyloFerm were compared with traditional baker’s yeast and baker’s yeast were surprisingly the fastest to ferment, but Ethanol Red had higher viability during fermentation. Protease addition during saccharification does not seem to improve fermentation with baker’s yeast. Prolonged liquefaction and saccharification time does probably not have any large impact on glucose yield. The continuous lab-scale process has a potential to be a realistic model but the stirring has to be improved and the pipe diameter increased.</p>

Bioethanol is a fuel of tomorrow, and progress in the use of enzymes and reduction of non-fermentable materials by debranning will probably be a part to make it more economical with low environmental impact.   Ethanol production based on debranned wheat was optimized in this study by batch experiments as well as continuous experiments in laboratory scale. Enzymes from Novozymes and Genencor were compared and no significant differences were discovered between the different set of enzymes. The yeast strains Ethanol Red and AmyloFerm were compared with traditional baker’s yeast and baker’s yeast were surprisingly the fastest to ferment, but Ethanol Red had higher viability during fermentation. Protease addition during saccharification does not seem to improve fermentation with baker’s yeast. Prolonged liquefaction and saccharification time does probably not have any large impact on glucose yield. The continuous lab-scale process has a potential to be a realistic model but the stirring has to be improved and the pipe diameter increased.

Proteins in Escherichia coli were compared in terms of essentiality, centrality, and conservation. The hypotheses of this study are: for proteins in Escherichia coli, (1) there is a positive, measureable correlation between protein conservation and essentiality, (2) there is a positive relationship between conservation and degree centrality, and (3) essentiality and centrality also have a positive correlation. The third hypothesis was supported by a moderate correlation, the first with a weak correlation, and the second hypotheis was not supported. When proteins that did not map to orthologous groups and proteins that had no interactions were removed, the relationship between essentality and conservation increased to a strong relationship. This was due to the effect of proteins that did not map to orthologus groups and suggests that protein orthology represented by clusters of orthologus groups does not accurately dipict protein conservation among the species studied.

過去漢語與格研究多圍繞在給字句的分析上，包括給字句的類別，以及不同類別中的「給」應為何種詞類。本文以當代與格結構的核心問題為起點，主張介詞與格和雙賓與格並不具備衍生關係，而是動詞的兩個面向──中心語PLoc／轉移性質（allative）或中心語PHave／所屬性質（possessive），所決定的兩種不同的論元結構和句法形式。以其觀點而論，漢語的介詞與格由前者形成，而雙賓與格由後者形成，而漢語與格的不對稱性，即所謂巴克詭題（Baker’s Paradox），亦可從前述兩個動詞面向加以解釋。本文認為漢語的給字句和把字句的語法形式，亦是前述兩個動詞面向的展現。並且，相關的分析不止適用於漢語與格類動詞，非與格類動詞亦能獲得闡述。

Cette thèse examine quelques approches aux équations diophantiennes, en particulier les connexions entre l’analyse diophantienne et la théorie des corps cyclotomiques.Tout d’abord, nous proposons une introduction très sommaire et rapide aux méthodes d’analyse diophantienne que nous avons utilisées dans notre travail de recherche. Nous rappelons la notion de hauteur et présentons le PGCD logarithmique.Ensuite, nous attaquons une conjecture, formulée par Skolem en 1937, sur une équation diophantienne exponentielle. Pour cette conjecture, soit K un corps de nombres, α1 ,…, αm , λ1 ,…, λm des éléments non-nuls de K, et S un ensemble fini de places de K (qui contient toutes les places infinies), de telle sorte que l’anneau de S-entiers OS = OK,S = {α ∈ K : |α|v ≤ 1 pour les places v ∈/ S}contienne α1 , . . . , αm , λ1 , . . . , λm α1-1 , . . . , αm-1. Pour chaque n ∈ Z, soit A(n)=λ_1 α_1^n+⋯+λ_m α_m^n∈O_S. Skolem a suggéré [SK1] :Conjecture (principe local-global exponentiel). Supposons que pour chaque idéal non-nul a de l’anneau O_S, il existe n ∈ Z tel que A(n) ≡0 mod a. Alors, il existe n ∈ Z tel que A(n)=0.Soit Γ le groupe multiplicatif engendré par α1 ,…, αm. Alors Γ est le produit d’un groupe abélien fini et d’un groupe libre de rang fini. Nous démontrons que cette conjecture est vraie lorsque le rang de Γ est un.Après cela, nous généralisons un résultat précédent de Mourad Abouzaid ([A]). Soit F (X,Y) ∈ Q[X,Y] un Q-polynôme irréductible. En 2008, Mourad Abouzaid [A] a démontré le théorème suivant:Théorème (Abouzaid). Supposons que (0,0) soit un point non-singulier de la courbe plane F(X,Y) = 0. Soit m = degX F, n = degY F, M = max{m, n}. Soit ε tel que 0 < ε < 1. Alors, pour toute solution (α, β) ∈ Q ̅2 de F(X,Y) = 0, nous avons soit max{h(α), h(β)} ≤ 56M8ε−2hp(F) + 420M10ε−2 log(4M),soitmax{|h(α) − nlgcd(α, β)|,|h(β) − mlgcd(α, β)|} ≤ εmax{h(α), h(β)}++ 742M7ε−1hp(F) + 5762M9ε−1log(2m + 2n)Cependant, il a imposé la condition que (0,0) soit un point non-singulier de la courbe plane F(X,Y) = 0. En utilisant des versions quelque peu différentes du lemme “absolu” de Siegel et du lemme d’Eisenstein, nous avons pu lever la condition et démontrer le théorème de façon générale. Nous démontrons le théorème suivant:Théorème. Soit F(X,Y) ∈ Q ̅[X,Y] un polynôme absolument irréductible qui satisfasse F(0,0)=0. Soit m=degX F, n=degY F et r = min{i+j:(∂^(i+j) F)/(∂^i X∂^j Y)(0,0)≠0}. Soit ε tel que 0 < ε < 1. Alors, pour tout (α, β) ∈ Q ̅2 tel que F(α,β) = 0, nous avons soith(α) ≤ 200ε−2mn6(hp(F) + 5)soit|(lgcd(α,β))/r-h(α)/n|≤1/r (εh(α)+4000ε^(-1) n^4 (h_p (F)+log⁡(mn)+1)+30n^2 m(h_p (F)+log⁡(mn) ))Ensuite, nous donnons un aperçu des outils que nous avons utilisés dans les corps cyclotomiques. Nous tentons de développer une approche systématique pour un certain genre d’équations diophantiennes. Nous proposons quelques résultats sur les corps cyclotomiques, les anneaux de groupe et les sommes de Jacobi, qui nous seront utiles pour ensuite décrire l’approche.Finalement, nous développons une application de l’approche précédemment expliquée. Nous considèrerons l’équation diophantienne(1) Xn − 1 = BZn,où B ∈ Z est un paramètre. Définissons ϕ∗(B) := ϕ(rad (B)), où rad (B) est le radical de B, et supposons que(2) (n, ϕ∗(B)) = 1.Pour B ∈ N_(>1) fixé, soit N(B) = {n ∈ N_(>1) | ∃ k > 0 tel que n|ϕ∗(B)}. Si p est un premier impair, nous appellerons CF les conditions combinéesI La conjecture de Vandiver est vraie pour p, c’est-à-dire que le nombre de classe h+ du sous-corps réel maximal du corps cyclotomique Q[ζp ], n’est pas divisible par p.II Nous avons ir(p) < √p − 1, en d’autre mots, il y a au plus √p − 1 entiers impairs k < p tels que le nombre de Bernouilli Bk ≡ 0 mod p. [...] / This thesis examines some approaches to address Diophantine equations, specifically we focus on the connection between the Diophantine analysis and the theory of cyclotomic fields.First, we propose a quick introduction to the methods of Diophantine approximation we have used in this research work. We remind the notion of height and introduce the logarithmic gcd.Then, we address a conjecture, made by Thoralf Skolem in 1937, on an exponential Diophantine equation. For this conjecture, let K be a number field, α1 ,…, αm , λ1 ,…, λm non-zero elements in K, and S a finite set of places of K (containing all the infinite places) such that the ring of S-integersOS = OK,S = {α ∈ K : |α|v ≤ 1 pour les places v ∈/ S}contains α1 , . . . , αm , λ1 , . . . , λm α1-1 , . . . , αm-1. For each n ∈ Z, let A(n)=λ_1 α_1^n+⋯+λ_m α_m^n∈O_S. Skolem suggested [SK1] :Conjecture (exponential local-global principle). Assume that for every non zero ideal a of the ring O_S, there exists n ∈ Z such that A(n) ≡0 mod a. Then, there exists n ∈ Z such that A(n)=0.Let Γ be the multiplicative group generated by α1 ,…, αm. Then Γ is the product of a finite abelian group and a free abelian group of finite rank. We prove that the conjecture is true when the rank of Γ is one.After that, we generalize a result previously published by Abouzaid ([A]). Let F(X,Y) ∈ Q[X,Y] be an irreducible Q-polynomial. In 2008, Abouzaid [A] proved the following theorem:Theorem (Abouzaid). Assume that (0,0) is a non-singular point of the plane curve F(X,Y) = 0. Let m = degX F, n = degY F, M = max{m, n}. Let ε satisfy 0 < ε < 1. Then for any solution (α,β) ∈ Q ̅2 of F(X,Y) = 0, we have eithermax{h(α), h(β)} ≤ 56M8ε−2hp(F) + 420M10ε−2 log(4M),ormax{|h(α) − nlgcd(α, β)|,|h(β) − mlgcd(α, β)|} ≤ εmax{h(α), h(β)}++ 742M7ε−1hp(F) + 5762M9ε−1log(2m + 2n)However, he imposed the condition that (0, 0) be a non-singular point of the plane curve F(X,Y) = 0. Using a somewhat different version of Siegel’s “absolute” lemma and of Eisenstein’s lemma, we could remove the condition and prove it in full generality. We prove the following theorem:Theorem. Let F(X,Y) ∈ Q ̅[X,Y] be an absolutely irreducible polynomial satisfying F(0,0)=0. Let m=degX F, n=degY F and r = min{i+j:(∂^(i+j) F)/(∂^i X∂^j Y)(0,0)≠0}. Let ε be such that 0 < ε < 1. Then, for all (α, β) ∈ Q ̅2 such that F(α,β) = 0, we have eitherh(α) ≤ 200ε−2mn6(hp(F) + 5)or|(lgcd(α,β))/r-h(α)/n|≤1/r (εh(α)+4000ε^(-1) n^4 (h_p (F)+log⁡(mn)+1)+30n^2 m(h_p (F)+log⁡(mn) ))Then, we give an overview of the tools we have used in cyclotomic fields. We try there to develop a systematic approach to address a certain type of Diophantine equations. We discuss on cyclotomic extensions and give some basic but useful properties, on group-ring properties and on Jacobi sums.Finally, we show a very interesting application of the approach developed in the previous chapter. There, we consider the Diophantine equation(1) Xn − 1 = BZn,where B ∈ Z is understood as a parameter. Define ϕ∗(B) := ϕ(rad (B)), where rad (B) is the radical of B, and assume that (2) (n, ϕ∗(B)) = 1.For a fixed B ∈ N_(>1)we let N(B) = {n ∈ N_(>1) | ∃ k > 0 such that n|ϕ∗(B)}. If p is an odd prime, we shall denote by CF the combined condition requiring thatI The Vandiver Conjecture holds for p, so the class number h+ of the maximal real subfield of the cyclotomic field Q[ζp ] is not divisible by p.II We have ir>(p) < √p − 1, in other words, there is at most √p − 1 odd integers k < p such that the Bernoulli number Bk ≡ 0 mod p. [...]

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