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Pressurized Mixtures of Ionic Liquids as Process Solvents for BiomassWilliams, Michael Lawrence 04 January 2021 (has links)
The present thesis investigates the application of pressurized mixtures of imidazolium-based ionic liquids with traditional organic solvents for the dissolution and extraction of lignocellulosic biomass, with bamboo as a specific example of renewable biomass. The approach has been unconventional in that the focus has been on solvent mixtures in which the ionic liquid is the minor component. The objective has been to combine the solvating power of the ionic liquid with a traditional solvent such as ethanol to modulate the outcomes of solubility and extractions by tuning the parameters of fluid composition, temperature, and pressure. Working with mixtures of ionic liquids in traditional solvents as process solvents lowers the viscosity of the medium and thus reduces the transport limitations that are often encountered when working with pure ionic liquids. Among other potential advantages are the reductions in overall process cost that are associated with ionic liquids, potentially easier recovery of post-extraction products, and the recycling of the ionic liquids. This thesis has also addressed another important question regarding the thermal stability of the ionic liquids as a processing medium at elevated temperatures and pressures over time, which may negatively impact their recovery and reuse, and may lead to environmentally unacceptable consequences. The dissolution experiments were carried out in a specially designed high-pressure view-cell equipped with sapphire windows for visual or optical observations. Evaluations were made employing standard characterization tools such as Thermogravimetric Analysis (TGA), Fourier Transform Infrared Spectroscopy (FTIR), UV-Vis Spectroscopy, and Scanning Electron Microscopy (SEM). Thermal stability studies were carried out using a combination of a view-cell and fiber optic UV-Vis capability at high pressures (up to 350 bar) and temperatures (up to 150 ℃).
The dissolution of bamboo was first explored using mixtures of 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac) with ethanol at temperatures from 100 to 150 ℃ and pressures from 35 to 350 bar over 4 or 24 h extraction times. The fluid mixtures employed were in the range of 1 - 40 wt % ionic liquid, which is in contrast to relevant dissolution experiments reported in the literature which either use pure ionic liquids or have the ionic liquids as the majority component. The effects of changing the temperature, pressure, and solvent composition on the removal of different components of the bamboo were assessed. Temperature played the most significant role in the amount of material extracted from the bamboo, with higher temperatures resulting in the removal of more lignin than cellulose and greater conversion of crystalline cellulose to the less recalcitrant amorphous form of cellulose. The concentration of ionic liquid in solution was also important, with higher concentrations resulting in more dissolved biomass. Finally, increasing the pressure resulted in higher amounts of dissolved biomass.
The next series of studies focused on rigorously assessing the stability of 1-alkyl-3-methylimidazolium acetate and chloride ionic liquids with alkyl chain lengths from 2 to 10 under both isothermal and non-isothermal conditions via thermogravimetric analysis. Isothermal degradation experiments were conducted at temperatures ranging from 100 to 225 ℃ over time periods ranging from two hours to three weeks. Non-isothermal degradation experiments were conducted at heating rates of 5, 10, 15, and 20 ℃/min from room temperature to 650 ℃. The activation energies and pre-exponential factors were assessed with isoconversional integral methods; the activation energies () ranged from 115 to 157 kJ/mol, and the pre-exponential factors (()) ranged from 24-38. The degradation reactions could be described as 1st order, as they often are in the literature, but were best fit by the 3-dimensional reaction model. Ionic liquids with longer alkyl chains on their imidazolium rings decomposed more quickly and at lower temperatures.
The thermal stability of the most promising ionic liquids ([EMIM]Ac, [BMIM]Ac, [EMIM]Cl, and [BMIM]Cl) were then assessed more closely at the possible biomass processing conditions that were being considered. The primary interest was determining the effects of various cosolvents on the thermal stability of these ionic liquids at the process temperatures and pressures, from 100 to 150 ℃ and 35 to 350 bar. These evaluations were carried out in the same high-pressure view cell in which the extraction experiments were conducted. To assess the degradation of the ionic liquids, time-evolved UV spectra of the mixtures were generated. It was found that more protic solvents such as water attenuated the degradation of the ionic liquids, whereas aprotic solvents such as DMF significantly exacerbated their degradation. Among the ionic liquids evaluated, it was found that [BMIM]Cl had the greatest stability in ethanol at 150 ℃.
The bamboo extraction experiments were then continued with mixtures of [BMIM]Cl in ethanol. The results showed that higher temperatures are necessary to extract lignin and cellulose, with [BMIM]Cl's thermal stability at these temperatures giving it the advantage over [EMIM]Ac. In this system as well it was shown that higher concentrations of ionic liquid facilitated the extraction of more biomass. However, biomass constituents that dissolve into mixtures with lower concentrations of ionic liquid readily precipitate back out of solution when the mixture is returned to room conditions. Along with the results of the studies with [EMIM]Ac, the experiments conducted with [BMIM]Cl show that an increase in pressure results in greater amounts of dissolved biomass holding other conditions constant.
The thesis, in summary, presents for the first time (a) the use of ionic liquids as a minor component in organic solvents as a potential biomass processing media, (b) the thermal stability of ionic liquids in a cosolvents at high pressures and temperatures, and (c) experimental results showing that pressure can enhance the amount that can be extracted from biomass with mixtures of ionic liquids in a cosolvent like ethanol. / Doctor of Philosophy / The purpose of the work detailed in the present thesis is to better understand the effects of mixtures of ionic liquids and traditional solvents on woody biomass. Ionic liquids are organic salts with melting points below 100 ℃, and they possess unique physical and chemical properties that can facilitate the dissolution or extraction of otherwise recalcitrant materials. There is a rapidly growing need for greener and more sustainable methods of processing woody biomass, which consist of primarily cellulose, lignin, and hemicelluloses. Industrial use of these liquids as processing solvents for woody biomass is limited by their relatively high viscosity, cost, and the difficulty of separating dissolved materials back out of solution. One method used to address these limitations is to mix the ionic liquids with other solvents, such as ethanol. The studies detailed in this thesis also seek to understand the effects of temperature and pressure on both the dissolution of woody biomass and on the degradation of the ionic liquids. The studies employ both traditional characterization equipment and a custom-designed view-cell which allowed for observation and characterization at high temperatures and pressures.
The first part of the study investigated the dissolution of bamboo with mixtures of the ionic liquid 1-ethyl-3-methylimidazolium acetate, [EMIM]Ac, and ethanol. The effects of changing the temperature, pressure, and solvent composition on the removal of different components of the bamboo were assessed. It was found that temperature played the most significant role in the amount of material extracted, with higher temperatures resulting in the removal of more lignin than cellulose. The concentration of ionic liquid in solution was also important, with higher concentrations resulting in more dissolved biomass. Finally, increasing the pressure resulted in higher amounts of dissolved biomass.
The next parts of the study focused on the degradation of the ionic liquids at elevated temperatures. The type of ionic liquids used in this study do not boil or evaporate at high temperatures, but instead break down into constituents that are themselves volatile. The thermal degradation of the ionic liquid used in the initial biomass dissolution experiments was investigated along with a series of similar ionic liquids. Their degradation behavior was assessed both by measuring their mass over time at a single constant temperature, and by heating them at a constant rate until they fully degraded. This behavior was mathematically modeled. The thermal stability of the most promising ionic liquids were then investigated in mixtures with other solvents in the high-pressure experimental cell under the same temperature and pressure conditions used in the biomass dissolution experiments.
The ionic liquid found to have the best stability in ethanol in those experiments was 1-butyl-3-methylimidazolium chloride, [BMIM]Cl. Further dissolution experiments were carried out with mixtures of this ionic liquid in ethanol. These experiments took the insights gained from the previous investigations to further clarify the effects of temperature, concentration, and pressure on the dissolution of bamboo in mixtures of ionic liquid and ethanol. It was again shown that higher temperatures are necessary to extract lignin and cellulose. It was also shown that higher concentrations of ionic liquid facilitate the extraction of more biomass. However, it was also shown that biomass dissolved into mixtures with lower concentrations of ionic liquid readily precipitates back out of solution when the mixture is returned to room conditions. Pressure was again shown to have a favorable effect on the amount of material extracted.
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Effect of Reactor Feeding Pattern on Performance of an Activated Sludge SBRCubas Suazo, Francisco Jose 06 December 2006 (has links)
The possible effects of changes in the feeding pattern on activated sludge properties related to bioflocculation have been analyzed in lab scale sequencing batch reactors (SBR) in order to determine if these changes in effluent water quality and settling and dewatering properties are significant, so they can be considered in future studies or if they can be recommended as crucial when operating and designing wastewater treatment plants. The activated sludge process is widely used to treat wastewater from both industrial and municipal sources. Biomass from industrial facilities containing high monovalent to divalent ion content usually settles poorly, which leads to low quality effluents that fail to meet environmental requirements. Therefore, the combined effect of feeding pattern plus the addition of sodium to activated sludge reactors was studied in this experiment.
A series of SBRs were operated at different sodium concentrations that ranged from 1.5 - 15 meq/L and different feeding times that ranged from 1 minute to 4 hours. Biomass samples were taken from each reactor to study the settling and dewatering properties and effluent samples were used to analyze the amount of organic matter and exocellular polymeric substances present due to deflocculation. As expected, the changes in feeding strategies affected all of the properties measured. When the feeding time was maintained low (pulse feed) the effluent quality and settling properties were the best. As the feeding time was increased the effluent quality, settling, and dewatering properties increased suggesting that the way in which the reactors were fed affected the overall bioflocculation process. The causes of the high deflocculation observed are not well understood, but data suggest that a microbial community change could have affected exocellular biopolymers which are believed to play an important role on bioflocculation.
This research demonstrates the importance of the interaction between cation content and feeding pattern when operating a wastewater treatment plants and when reporting lab-scaled results related to settling and bioflocculation. / Master of Science
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The influence of nitrogen and sludge age change in reactor performance and biopolymer production in activated sludgeLee, Samuel H. January 1986 (has links)
This study investigated the influence of nitrogen and sludge age change in reactor performance and biopolymer production in activated sludge systems. The qualitative and quantitative analyses of the naturally occurring biopolymers were performed and the results were correlated to sludge a settling characteristic and effluent quality.
In order to obtain the sludge samples for the analyses, two completely mixed, continuous flow activated sludge systems were maintained during this research. Raw wastewater from the Celanese Fiber Plant located at Narrows, Virginia was utilized as the influent. Nitrogen was added in the feed solution as ammonium sulfate. The sludge age was changed from ten to five days for both systems.
Biopolymers were extracted from the sludge floc matrix using pH-adjustment technique followed by centrifugation. The total biopolymer contents were analyzed for protein and carbohydrate concentrations. High molecular weight biopolymers were also analyzed following gel filtration. The sludge settling characteristics were measured in terms of Sludge Volume Index and effluent quality in terms of effluent turbidity.
The results indicated that the relationship between total biopolymer concentrations and sludge settling characteristics is culture specific. No consistent relationship was observed between total biopolymer concentrations and effluent turbidity and/or SVI.
Additional nitrogen in a reactor system promoted production of high protein content biopolymers. However, no significant improvement in effluent quality of the reactor was noticed by the additional nitrogen. Deficiency of nitrogen in a reactor system promoted the production of high carbohydrate content biopolymers. The high concentration of carbohydrate biopolymers seemed to correspond directly to the high effluent turbidity. / M.S.
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Dynamic Mechanical Properties of Cockroach(Periplaneta americana) ResilinChoudhury, Udit 01 March 2012 (has links)
Resilin is a cuticular protein found in a variety of insects. It can stretch up to 300% of its natural length without any creep or relaxation. Further, it operates across a wide frequency range from 5 Hz in locomotion to 13 kHz in sound production. Both the protein sequence and composition of natural resilin as well as the dynamic mechanical properties vary substantially across species. This suggests that mechanical properties may be evolutionarily tuned for specific functions within an insect. Here, samples of resilin obtained from the tibia-tarsal joint of the cockroach, Periplaneta americana, were tested using a custom built dynamic mechanical analyzer. The material properties in compression are obtained from the rubbery to glassy domain with time-temperature superposition (-2C to 55C) and time-concentration superposition (0 % to 93% ethanol by volume in water). At low frequency the storage modulus was found to be 1.5 MPa increasing to about 5 MPa in the transition zone. The glass transition frequency at 23C in complete hydration was found to be 200 kHz. The data shows that cockroach resilin is less resilient than dragonfly resilin at low frequencies, returning about 79% of the elastic strain energy at 25 Hz compared to 97% for dragonfly resilin. However, at the glass transition (200 kHz) the material returns about 47% of the elastic strain energy compared to 30% in dragonfly (2MHz ). The resilin pad in cockroach is a composite structure, acting as a compressive spring to passively extend the tibia-tarsal joint during cockroach locomotion. Its mechanical properties are more similar to the composite locust pre-alar arm than to the pure resilin dragonfly tendon, suggesting that macroscopic structural influences may be as important as molecular sequence differences in setting properties. / Master of Science
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Novel Materials for Neural Interface DevicesRauhala, Onni January 2024 (has links)
Developments in materials science and engineering have significantly enhanced the recording and stimulation capabilities of electrophysiological neural interface devices. Enhanced biocompatibility has increased the viability and longevity of such systems, with particularly interesting advances resulting from the utilization of organic and mixed-conductive conjugated polymers. These materials tend to improve biocompatibility and signal quality by overcoming material limitations of conventional metallic electrodes. Simultaneously, advanced microfabrication methods have increased the spatiotemporal resolution and signal quality of recorded data without added invasiveness.
The research work presented in this dissertation touches upon three aspects of relevance for improved neural interface use: i) improving the accuracy of histological verification procedures in research using naturally abundant organic materials; ii) introducing unconventional electrodes for neural recordings and localized drug delivery by utilizing conductive organic polymers and clinical supply items; iii) applying a high-density electrocorticography (ECoG) array to study differential neural oscillation patterns underlying memory processing in vivo.
First, we showcase a chitosan (CS) based, solution-processable film for localizing neural implants by leveraging CSs intrinsic fluorescence, without impeding data quality or cell viability.
Second, we develop a mixed-conductive suture by using standard silk sutures and the mixed-conductive polymer PEDOT:PSS. The resulting device (E-Suture) is shown to safely interface with live tissue and possess high-fidelity recording and stimulation capabilities as well as applicability for localized drug delivery thanks to the mixed-conductivity of PEDOT:PSS.
Finally, we leverage high spatiotemporal resolution ECoG arrays to show that distinctive oscillatory memory biomarkers in the neocortex and hippocampus show significant but differential temporal coupling patterns in response to consolidation of new information and reconsolidation of that information at a later time in rats.
This dissertation demonstrates the utility of different organic materials for the enhancement of neural interface functions at multiple phases of the device life cycle as well as a concrete demonstration of improved electrophysiological recording devices in answering key questions of foundational neuroscience.
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Development of novel bio-derived polymer composites reinforced with natural fibres and mineral fillersShakoor, Abdul January 2013 (has links)
Biocomposites exhibit properties like many petrochemical-based polymers composites. They have the potentials be used in the automotive and decking industries and as biodegradable packaging. However, the high cost as well as, poor mechanical and thermal properties have restricted their widespread use. There are a number of technical issues that need to be addressed before bio-composites can be widely used. In this research Polylactic acid (PLA) composites, reinforced with natural fibres (wood, flax) and mineral fillers (talc) were investigated. The thermal and mechanical properties of the composites were studied by means of Differential Scanning Calorimetry (DSC), Tensile Testing and Dynamic Mechanical Analysis (DMA), while morphology and crystallization processes of the composites were studied by hot stage optical microscopy. The experimental results are also compared with different theoretical models of the response of the composites. PLA / wood composites were developed by mixing PLA with wood in different ratios using a melt compounding process. PLA/wood (90/10. 80/20, 60/40), PLA/wood/copolymers (85/10/05, 80/10/10, 75/20/05, 70/20/10, 55/40/05, 50/40/10) and PLA/wood/coupling agent (80/20/silane coating) were the three different composite systems that were developed. Adding increasing amount of wood into the PLA, the thermal properties remain unchanged but the mechanical properties increased significantly, bringing a stiffening effect to the composites. Tensile modulus increased from 4.1± 0.6 to 9.8 ± 1.2 (GPa) as the wood content increased from 0 to 40 (wt %), but the tensile strength at break reduced from 43.8 ± 3.1 to 31.8 ± 2.8 MPa. The experimental results of the PLA-wood composites were modelled according to the Halpin-Tsai equation. The addition of copolymer affected the thermal properties considerably by decreasing the glass transition temperature of the composite. The glass transition temperature dropped from 54 ± 0.7 (0C) to 48 ± 0.36 (0C) when the content of copolymer was increased from 0 to 10 (wt %). The cold crystallization temperature also decreased from 127 ± 1.41 (0C) to 103 ± 2.58 (0C) when the copolymer was incorporated into the PLA/wood composites. The significant aspect was the occurrence of a double peak in the melting endotherm. The degree of crystallinity also increased from 2 ± 0.83 (%) to 11 ± 1.23 (%) when the amount of copolymer was increased to 10 (wt %). PLA, flax and expoidizied natural rubber (ENR) composites were also developed using a melt compounding process. The mechanical properties were affected significantly when the flax fibres were mixed with PLA in the ratios of 10, 20 and 30 (wt %). Addition of flax fibres increased the elastic modulus significantly but reduced the tensile strength and strain at break. To improve the toughness of the PLA- Flax composites, ENR was incorporated into the PLA- Flax composites. In order to balance the modulus of the reinforcement and the matrix, the PLA- Flax and ENR composites were annealed above the glass transition temperature and the degree of crystallinity increased from 2 to 35 (%). The integral blending of PLA, Flax and ENR did not affect the brittle fracture but introducing a masterbatch of flax fibres and ENR into the PLA matrix during melt processing had a considerable effect on the fracture behaviour of the composites. The elastic modulus of the composites decreased due to the elastomeric content in the composites and there was an increase in elongation-to-break. The effect of talc on the crystallinity and mechanical properties of a series of polylactic acid (PLA) / talc composites was investigated. PLA talc composites were developed by incorporating different types of the talc into the PLA in the ratios of 10, 20 and 30 (wt %). The composites were prepared by melt blending followed by compression moulding. It was found that talc acted as a nucleating agent and increased the crystallinity of the PLA from 2% to 25%. There was significant improvement in Young s modulus of the composites with increasing talc addition and these results were found to fit the Halpin Tsai model. Thermo-mechanical tests confirmed that the combination of increased crystallinity and storage modulus leads to improvement in the heat distortion properties.
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A methodology for evaluating multiple mechanical properties of prototype microfibrillated cellulose/poly(lactic acid) film compositesDing, Jie 08 September 2011 (has links)
The context of this thesis is a research project focused on the investigation of a renewable biopolymer-poly(lactic acid) (PLA) as a potential replacement of petroleum-based polymers in advanced nanocomposites reinforced with Microfibrillated cellulose (MFC). MFC is extracted from wood, which is a renewable, sustainable, carbon neutral and recyclable material. This advanced MFC-PLA bio-
based composite material is expected to allow for the substitution of petroleum-based plastics in various markets and applications.
The specific objectives of the thesis are: 1) to describe the morphological characterization of MFC used for prototype MFC-PLA composites, and 2) to determine the mechanical properties of the prototype MFC-PLA nanocomposites formulation generated in form of thin transparent films.
In order to meet this objective it was necessary to: 2.1) develop a methodology for optical strain measurement in transparent thin films; and 2.2) develop an effective methodology for obtaining multiple mechanical properties from small number of specimens of prototype materials subjected to tensile tests.
Two types of MFC, one obtained by courtesy of University of Maine and the other purchased from Innventia AB company, were investigated under a field emission scanning electron microscopy (FESEM). The micrographs obtained from FESEM showed that both types of MFC were of complex hierarchical structures, which did not allow qualitative characterization of the morphological features in terms of particulate composites nor cellular solids.
Since prototype formulations of MFC-PLA composites were generated in small amounts (typically one Petri dish) in a form of thin transparent films, there was a need for quick and efficient assessment of their key mechanical properties that would provide feedback and guide further prototyping work. An optical measurement method based on digital image correlation (DIC) principle was developed to measure the deformation and strains of the tensile film samples. In our study, the accuracy and precision of the measurement of deformation were ±1.5 µm and 0.4 µm respectively. The corresponding accuracy and precision in terms of strains were ±30 µstrain and 75 µstrain respectively. This method can be successfully used to determine the critical mechanical properties, such as elastic modulus, toughness and Poisson's ratio, of transparent thin films by a single tensile test, all of which require precise strain measurement.
In addition, this optical measurement method makes it possible to significantly simplify the testing for measuring essential work of fracture (EWF), an important material property of thin transparent films. In traditional method, measurement of EWF requires large amount of notched specimens. However, our study showed that only a small amount of notched specimens were needed to measure the EWF of a material. This method could not be successfully used to determine EWF from un-notched tensile specimens. / Graduation date: 2012 / Folder labeled "UMaine MFC aerogel" contains SEM micrographs of MFC from University of Maine (referred as type A MFC in the thesis). Two pieces of leaf-like flakes at different locations were cut by Focused Ion Beam (FIB) in order to observe the internal structure of the flakes.
Folder "FIB_01 ": a series of SEM micrographs of FIB-cut flake at different magnification levels.
Folder "FIB_02 ": another series of SEM micrographs of FIB-cut flake at various magnification levels.
Folder labeled "Swedish MFC aerogel" contains SEM micrographs of MFC from Innventia AB company, Sweden (referred as type B MFC in the thesis). There is a series of SEM micrographs of type B MFC aerogel at various magnification levels in this folder.
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Bioprospecção de genes relacionados à biossíntese de polímeros biodegradáveis a partir de uma biblioteca metagenômica de solo de Mata Atlântica. / Bioprospecting in a metagenomic library from Atlantic forest soil for genes involved on the biosynthesis of biodegradable polymers.Rozo, Yeimy Paola Galindo 10 November 2011 (has links)
O presente estudo teve como objetivo realizar a triagem de PHA sintases numa biblioteca metagenômica de solo de Mata Atlântica, empregando duas técnicas de busca: o método de detecção fenotípica e a técnica de PCR. Resultados positivos foram obtidos com este último, empregando iniciadores descritos na literatura e iniciadores descritos neste trabalho. Em 10.67% dos clones da biblioteca foram obtidos amplicons, dos quais 7 foram seqüenciados, apresentando similaridade para os genes phaC dos tipos II e IV. Adicionalmente, 67 clones positivos para a classe III foram obtidos e 4 destes foram seqüenciados. Duas das seqüências obtidas mostraram alta similaridade com o gene codificador da enzima glutamina sintetase tipo I, outro deles para a proteína hipotética conservada pertencente à família de enzimas oxidoredutases e a outra para o componente D do gene hidrogenase-4. A partir da análise dos resultados, iniciadores mais específicos são propostos. Assim, a técnica de PCR foi mais eficiente na detecção de genes da biossíntese de PHA na biblioteca metagenômica estudada. / To perform a PHA synthase screening in a metagenomic library from Atlantic forest soil two search methods were applied: phenotypic detection and PCR. Positive results with PCR were obtained by using primers described in the literature and proposed in this study. Amplicons were obtained in 10.67% of the library, 7 of them were sequenced showing similarity with class II and IV phaC genes. In addition, 67 positive clones for class III were obtained and 4 of them were sequenced. Two of these sequences showed high similarity to the glutamine synthase gene type I, the third one showed similarity to the conserved hypothetical protein of the reductase family, and the forth presented similarity to the component D of the hidrogenase-4. According to the results, more specific primers are suggested. Therefore, PCR was more efficient in the detection of PHA biosynthesis genes in the studied metagenomic library.
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Construção de bactérias recombinantes para produzir etanol e biopolímeros a partir de açucares derivados do hidrolisado do bagaço de cana-de-açúcares. / Engineering bacteria to produce ethanol and biopolymers using sugars derived from sugarcane bagasse hydrolysate.Freire, Rominne Karla Barros 05 June 2012 (has links)
Resíduos lignocelulósicos são substratos proeminentes para a produção sustentável de polihidroxialcanoatos (PHAs) e etanol. A xilose é um dos principais componentes da lignocelulose, mas o aproveitamento eficiente desse açúcar ainda representa uma barreira técnica. O objetivo desse trabalho foi obter linhagens bacterianas mais eficientes no consumo desse açúcar. Foi introduzido maior número de cópias dos genes do catabolismo (xylAB) e transporte (xylFGH) de xilose, nas linhagens Escherichia coli KO11, produtora de etanol, e Burkholderia sacchari LFM 101, produtora de poli-3-hidroxibutirato (PHB). Os recombinantes foram avaliados quanto ao consumo de xilose e produção na presença e ausência de glicose. Para B. sacchari LFM 101 essa estratégia não incrementou o consumo desse açúcar. Para E. coli KO11 xylAB reduziu o tempo de consumo de xilose e aumentou a produção final de etanol em 30%, mas esse efeito foi prejudicado pela repressão catabólica; enquanto xylFGH foi deletério ao reduzir para quase zero o crescimento e produção de etanol por essa linhagem. / Lignocellulosic residues are remarkable substrates for the sustainable production of polyhydroxyalkanoates (PHAs) and ethanol. Xylose is one of the most important lignocelullose component but its efficient utilization still represents a technical barrier. The aim of this work was to obtain bacterial strains more efficient in the xylose consumption. Multiple copies of the catabolism (xylAB) and transport (xylFGH) genes of xylose were introduced in the ethanol producer Escherichia coli KO11 strain and the poly-3-hydroxybutyrate (PHB) producer Burkholderia sacchari LFM 101. The recombinants strains were evaluated for their production and xylose consumption in the presence and absence of glucose. This strategy did not increase xylose consumption in B. sacchari strains. The xylAB gene improved xylose consumption and increased the ethanol production about 30% in E. coli KO11, but this effect was impaired by catabolite repression; while xylFGH gene was deleterious to reduce the growth and ethanol production by this strain.
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Síntese e avaliações físico-químicas de quitosanas quimicamente modificadas pela inserção de radicais de anidrido succínico / Synthesis and physico-chemical evaluations of chitosan chemically modified by the insertion of succinic anhydride radicalsKarine Gargioni Pereira Correa de Mello 01 September 2005 (has links)
A N-succinil-quitosana é um derivado quimicamente modificado do polímero quitosana. A inserção de radicais de anidrido succínico nas aminas protonadas presentes ao longo da cadeia do polímero quitosana, conferem diferentes características físico-químicas à molécula de quitosana. Esta modificação química possibilitou à quitosana, solubilidade em pHs que variam do ácido (2.0) até alcalino (14.0). Estas propriedades são atribuídas ao alongamento da cadeia alquílica, que afasta a ponte hidrofílica da cadeia fechada da D-glicosamina, facilitando o acesso da água, a qual irá estabelecer uma interação mais forte com a molécula de quitosana. Esta propriedade não está presente em amostras de quitosana pura, a qual sabe-se que solubiliza-se apenas em pHs abaixo de 5.5. / The N-succinil-chitosan is a chemically modified derivative of the biopolymer chitosan. The succinic anhydride attached to the free amino groups presented along the chitosan\'s polymer chain imparts to the molecule different physicochemical properties not exhibited before the modification. These chemical modifications enhance chitosan\'s solubility in slightly acid, neutral and alkaline media. These properties are related to the long alkyllic chains attached to hydrophilic parts. In this case the hydrophilic part of D-¬glucosamine promotes stronger interactions with the water molecules, and consequently, enhances the solubility of the chitosan polymer. It is worthy mentioning that non-modified free chitosan is soluble only in acidic medium (pH ≤5.5).
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