This thesis deals with the design and implementation of a self-driving model race car that is intended to attend an NXP Cup race. The work describes the selection of the platform, design process of algorithm, design process of printed circuit board for obstacle detection system, and various experiments with motion control.

Current systems for counting cars on parking lots usually use specialized equipment, such as barriers at the parking lot entrance. Usage of such equipment is not suitable for free or residential parking areas. However, even in these car parks, it can help keep track of their occupancy and other data. The system designed in this thesis uses the YOLOv4 model for visual detection of cars in photos. It then calculates an embedding vector for each vehicle, which is used to describe cars and compare whether the car has changed over time at the same parking spot. This information is stored in the database and used to calculate various statistical values like total cars count, average occupancy, or average stay time. These values can be retrieved using REST API or be viewed in the web application.

The aim of this diploma thesis is to draw up a business plan regarding an extension of an existing car repair service by adding a public fuel filling station to it. The public fuel filling station will be built directly within the area of the existing car repair service located in the village of Jemnice, in the district of Žďár nad Sázavou. The theoretical part generally describes the business activity, possible forms of business and individual analyses used in this diploma thesis. The second part briefly describes the chosen company, for which the above mentioned draft of the businnes plan is intended. The last part of the diploma thesis presents the actual construction plan of the public fuel filling station based on the elaborated analyses, including the time schedule of the execution.

The Master’s thesis deals with development of universal equipment for testing and measurement operating forces of car seats. It describes tests applied on car seats and shows a few examples of equipment for testing. The design part of the thesis deals with development of multipurpose testing equipment using of Modular Function Deployment™ approach. The design of the equipment is done in 5 steps according to mentioned approach. The final part of the thesis includes a risk analysis of the new device and conclusion.

This diploma thesis studies the use of air springs for race car, precisely Formula Student race car. Research part of the thesis deals with choosing suitable air spring for given application. In practical part, there is computation of parameters for appropriate setting of air spring, so it will comply with the rules of Formula Student. There is also simulation of air spring functionality in real use.

This master's thesis is about nature optimalization technigues. Evolution algortihms together with main thesis topic, Particle Swarm Optimization, is introduced in the following chapter. Car setup and simulator TORCS are introduced in next chapter. Design and implementation are introduced in next chapters. Destination of t master's thesis is finding optimal car setups for different curcuits.

The aim of this thesis is to create a cloud application for traffic analysis without knowing anything about the system. The only input is address of the web camera pointing at traffic. This application is build on existing solution which is further enhanced. New modules for removing obstacles (such as lamppost covering part of the road) and splitting overlapping cars were added. The whole cloud solution consists of multiple components which communicates by HTTP messages and are controlled by web interface.

Inter-Vehicular Communication is an optimistic technology to enhance road safety. The wireless medium often used to exchange vital information. Wi-Fi is also used to share the information, but it required an access point hardware to communicate. Wi-Fi Direct enabled the device exchange information without a hardware access point. Over the last decades, the usage of wireless technology has been increased and transformed the way electronic devices interact and communicate. Wi-Fi Direct technology provides peer-to-peer communication. So, Wi-Fi is highly applicable for car-to-car communication because of its High data rate, range, standard usage. Within the framework of this thesis concept of prototyping car-to-car communication by using Wi-Fi Direct service in smartphones. Focus here to optimize resource utilization and to provide basic functionality within the programming mode.

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CAR-T-Zell-Therapie ist eine vielversprechende neuartige Behandlungsform für Patienten mit aggressiven B-Zell Non-Hodgkin-Lymphomen (B-NHL). In dieser Arbeit wurde die anti-CXCR5 CAR-T-Zell-Therapie als Alternative zur anti-CD19 CAR-T-Zell-Therapie für die Behandlung von reifen B-NHLs untersucht. CXCR5 ist ein B-Zell-homing Rezeptor, der von reifen B Zellen und follikulären T-Helferzellen (TFH Zellen) exprimiert wird. TFH Zellen wurden als tumor-unterstützend in chronisch lymphatischer Leukämie (CLL) und im follikulären Lymphom (FL) beschrieben. Dieses Expressionsmuster erlaubt es, auf einzigartige Weise zeitgleich die malignen Zellen und die tumorunterstützende Mikroumgebung mithilfe von CAR-T-Zell-Therapie gerichtet gegen einen Chemokinrezeptor anzugreifen. Die wichtigsten Ergebnisse dieser Arbeit waren, dass (1) die anti-CXCR5 CAR T-Zellen zielgerichtet CXCR5 positive reife B-NHL Zelllinien und Patientenproben in vitro eliminierten und eine starke anti-Tumor Reaktivität in einem immundefizienten Xenotransplantationsmausmodell zeigten, (2) die anti-CXCR5 CAR T-Zellen zielgerichtet die tumorunterstützenden TFH Zellen in CLL und FL Patientenproben in vitro erkannten und dass (3) CXCR5 ein sicheres Expressionsprofil zeigte. CXCR5 war stark und häufig auf B-NHL exprimiert und die Expression auf gesundem Gewebe war auf lymphoide Zellen beschränkt. Zusammenfassend lässt sich sagen, dass die anti-CXCR5 CAR-T-Zell-Therapie eine neue Behandlungsmöglichkeit für Patienten mit reifen B-NHL darstellt, indem durch die anti-CXCR5 CAR-T Zellen sowohl der Tumor als auch ein Anteil der tumorunterstützende Mikroumgebung eliminiert werden. Im zweiten Teil der Arbeit wurde das Eμ-Tcl1 murine CLL Lymphommodell genutzt um die Auswirkung der Lymphomentwicklung auf die CXCR5+ T Zellen zu untersuchen. Mittels RNA-Einzelzell-Sequenzierung konnte ein profunder Einfluss des Lymphomwachstums auf das T Zell-Kompartiment der Mäuse, denen Eμ-Tcl1 Zellen gespritzt wurden, gezeigt werden. / CAR T cell therapy is a promising new treatment option for patients suffering from aggressive B non-Hodgkin lymphomas (NHLs). In CAR T cell therapy, patient-derived T cells are genetically modified to express a chimeric receptor commonly directed towards a surface antigen expressed by neoplastic cells. In this thesis, anti-CXCR5 CAR T cell therapy was investigated as an alternative to anti-CD19 CAR T cell therapy for the treatment of mature B-NHLs. CXCR5 is a B cell homing receptor expressed by mature B cells and follicular helper T (TFH) cells. TFH cells were described to support the tumor cells in chronic lymphocytic leukemia (CLL) and follicular lymphoma (FL). This expression pattern allows simultaneous targeting of the malignant cells and the tumor-supporting microenvironment by CAR T cell therapy against a chemokine receptor in an unprecedented manner. Main findings included that (1) anti-CXCR5 CAR T cells targeted specifically CXCR5 expressing mature B-NHL cell lines and patient samples in vitro and showed strong in vivo anti-tumor reactivity in an immunodeficient xenograft mouse model, (2) anti-CXCR5 CAR T cells targeted tumor-supportive TFH cells derived from CLL and FL patient samples in vitro and (3) CXCR5 showed a safe expression profile. CXCR5 was strongly and frequently expressed by B-NHLs and its expression on healthy tissue was restricted to lymphoid cells. In summary, anti-CXCR5 CAR T cell therapy presents a novel treatment option for patients suffering from mature B-NHLs by eliminating the tumor and part of the tumor-supportive microenvironment. The second part of the project, the Eμ-Tcl1 murine lymphoma model, which mimics human CLL, was used to study the impact of lymphomagenesis on CXCR5+ T cells. Using single cell RNA sequencing, a profound influence of lymphoma growth on the T cell compartment in Eμ-Tcl1 tumor-challenged mice could be shown.