This diploma thesis deals with a car in a relation to accounting and tax laws. This thesis is divided into two basic parts, theoretical and practical. The theoretical part is concerned with a car perception in accounting and the influence of tax laws on the car perception. The theoretical part is divided into three main parts. The first part is focused on a general view of vehicles in accounting, for example, vehicle valuations, acquisitions, depreciation, valuation changes and disposals of vehicles. The other part is focused on specifications of the vehicle perception in a tax evidence of individual entrepreneurs. In the third part there are described the vehicle perception in business corporations. It is focused on a value-added tax, mainly on a value-added tax in cross-border buying and selling of vehicles. Furthermore, there are analyzed a deferred tax and issues of cars in a relation to employers and employees. In the theoretical part there is dealt with the usage of vehicles for private purposes. In the practical part there is analyzed the usages of company cars by an employee for private purposes. There is compared a rent and a free of charge usage. In this part there is also verified whether the law is followed in practice. There are stated atypical transactions. These include using grants to acquire a car, updating of a depreciation plan and disposing of a vehicle by its sale to a foreign entity.

This thesis deals by the design of 1/4 car model for testing vehicle dampers, which can be used to simulate the real suspension of a vehicle wheel (up to a maximum car weight of 1,970 kg) and the so-called linear wheel suspension. A linear mathematical 1/4 car model with 2 DOF (Degrees Of Freedom) and data from literature search are used to design and derive the basic parameters of the device. The thesis contains a description of the linear mathematical model and its outputs (acceleration of the sprung mass and forces acting on the sprung mass), description of designed device, descriptions of created simulations (static, modal and harmonic analysis in ANSYS Workbench 2020 R2) and conceptual design of the modifications this device for another possible use for testing of bicycles.

Tato práce popisuje simulátor TORCS a optimalizační algoritmy, jenž jsou využívány při tvorbě autonomních řidičů pro tento simulátor. Hlavním cílem je navržení nového autonomního řidiče, který se bude schopen s použitím přírodou inspirovaných optimalizačních technik vyrovnat již dříve navrženým řešením. Chování implementovaného řešení lze rozdělit do dvou hlavních částí, které jsou využívány v různých rozdílných etapách závodu. Zahřívací kolo je využito pro vytvoření modelu trati, ze kterého je posléze získána optimální trajektorie pomocí genetického algoritmu. Této trajektorie je potom využíváno v samotné kvalifikaci či závodě pro zajetí co nejrychlejšího kola. Z důvodu složitosti problému optimalizace celé trajektorie je nutno tuto trajektorii rozdělit na menší úseky nazývané segmenty, přičemž každý z nich je potom optimalizován odděleně. Jednotlivé optimalizované segmenty jsou následně spojeny dohromady, aby opět utvořily trajektorii pro celou trať. Protože některé přechody mezi segmenty mohou být nesouvislé, je zde znovu aplikován genetický algoritmus pro jejich vyhlazení. Během závodu je tato trajektorie následována, přičemž se z ní odvíjí i maximální možná rychlost v daném úseku. V práci jsme ukázali, že vzorkování trati s následnou optimalizací pomocí genetického algoritmu trvá pouze zlomek času vyhrazeného pro zahřívací kolo. Nejen díky tomuto se řešení jeví jako vhodné pro závody autonomních řidičů a může být dále rozšířeno.

Background: The CD19 chimeric antigen receptor (CAR) adoptive T-cell therapy for B-cell leukemia is a promising treatment for relapsed or refractory malignities. The overall response rate of CD19 CAR-T cells in clinical trials was greater than 80% for patients with B-cell acute lymphoblastic leukemia (B-ALL) and non-Hodgkin's lymphoma (NHL). However, CAR-T cell therapy of leukemias and solid tumors has been limited by a lot of factors such as antigen loss of tumor escape variants, reduced proliferation, persistence and tumor-infiltration of CAR-T cells in vivo, immunosuppressive tumor environment, absence of ideal antigens and on-target, off-tumor toxicities. Therefore, new strategies improving the safety and efficacy of CAR-T cells, including further T-cell modification to overcome the immune suppression, are tested. Aims: (i) Bispecific CARs designed to express two antigen-binding domains prevent of antigen escape. (ii) T-cells were genetically modified to express CAR along with an inducible IL-21 gene cassette driven by NFAT-responsive promoter. IL-21 directly enhances CAR-T cell activity and anti-tumor effects. (iii) Applying suicide epitope modification in CAR enables significantly increasing the therapeutic safety of CAR-T cells. Methods: CARs were constructed by using molecular biology...

Cancer immunotherapies utilize the capabilities of the immune system to efficiently target malignant cells. In recent years, chimeric antigen receptor (CAR) equipped T cells showed promising results against B cell lymphomas. Autologous CAR-T cells require patientspecific manufacturing and thus extensive production facilities, resulting in high priced therapies. Along with potentially severe side effects, these are the major drawbacks of CAR-T cells therapies. Natural Killer (NK) cells pose an alternative for CAR equipped immune cells. Since NK cells can be safely transferred from healthy donors to cancer patients, they present a suitable platform for an allogeneic “off-the-shelf” immunotherapy. However, administration of activated NK cells in cancer therapy has until now shown poor anti-cancer responses, especially in solid tumors. Genetic modifications such as CARs promise to enhance recognition of tumor cells, thereby increasing anti-tumor effects and improving clinical efficacy. Although the cell biology of T and NK cells deviates in many aspects, the development of CAR-NK cells frequently follows within the footsteps of CART cells, meaning that T cell technologies are simply adopted to NK cells. In this review, we underline the unique properties of NK cells and their potential in CAR therapies. First, we summarize the characteristics of NK cell biology with a focus on signaling, a fine-tuned interaction of activating and inhibitory receptors. We then discuss why tailored NK cellspecific CAR designs promise superior efficacy compared to designs developed for T cells. We summarize current findings and developments in the CAR-NK landscape: different CAR formats and modifications to optimize signaling, to target a broader pool of antigens or to increase in vivo persistence. Finally, we address challenges beyond NK cell engineering, including expansion and manufacturing, that need to be addressed to pave the way for CAR-NK therapies from the bench to the clinics.

Whiplash is a neck injury caused by the sudden differential movement between the head and torso. Whiplash injuries are most commonly reported as a consequence of rear impacts in car accidents. They are regarded as minor injuries, but can still lead to long-term disablement and discomfort in the neck. Whiplash injuries can be mitigated by better car seat designs. For this purpose, head restraint geometry must be improved first, and then the dynamic performance of the whole seat must be assessed at all crash seventies. A biofidelic human-body model is a key requirement in designing whiplash mitigating car seats. This thesis presents the development of a 50th percentile male multi-body human model and several energy absorbing car seat designs. The human-body model is specifically designed for rear impact and validated using the responses of seven volunteers from Japanese Automobile Research Institute (JARI) sled tests, which were performed at an impact speed of 8 kph with a rigid seat and without head restraint and seat belt. A generic multi-body car seat model is also developed to implement various seatback and recliner properties, anti-whiplash devices (A WDs) and head restraints. Using the same driving posture and the rigid seat in the JARI sled tests as the basic configuration, several anti-whiplash seats are designed to allow different types of motion for the seatback and seat-pan. The major findings of this research are: -The human-body model simulates the effects of muscle contraction and its overall response is superior in comparison to the currently used models and dummies. -A criterion called the S-shape index (SSI) is developed based on the intervertebral angles of the upper and lower cervical spine. -The car seat design concepts are able to control and use crash energy effectively with the aid of anti-whiplash devices for a wide range of crash seventies. -In order to reduce whiplash injury risk, this study advocates energy absorbing car seats which can also provide head restraint contact as early as possible.

Målet med examensarbetet har varit att samla information åt ett tekniskt konsultföretag för att öka deras kunskap om autonoma system och fordonskommunikation. Statusen på arbetet kring dessa aktiva säkerhetssystem hos olika aktörer och hur systemen implementeras i dagens och framtidens fordon har undersökts genom omfattande litteraturstudier, intervjuer och marknadsanalyser. De autonoma systemen kan samla information från omgivningen genom sensorer och bidra till ett jämnare trafikflöde, ökad säkerhet, lättare bilar och bättre miljö. Genom fordonskommunikationen kan fordon kommunicera med varandra samt infrastrukturen och garantera en säker bilfärd. År 2030 utgörs innerstaden av autonom, elektrifierad kollektivtrafik för att transportera människor på begäran, samtidigt som personbilar till viss del förbjuds. Potentiella behov för människan i en fullt autonom bil har identifierats och diverse produktutvecklingsmetoder har tillämpats för att utforma två konceptuella lösningar för en framtida bilinteriör. Lösningarna visar interaktionen mellan människa och system eftersom underhållning och bekvämlighet blir viktigt i en fullt autonom bil. Respektive lösning är statsägd och rymmer fyra passagerare. I lösningarna är sittplatserna placerade på ett sätt som underlättar kommunikation mellan passagerarna. Passagerarna kan underhållas eller informeras individuellt eller gemensamt via text, ljud och bild. / The goal with this thesis project has been to collect information for a technical consulting company in order to increase their knowledge about autonomous systems and vehicular communication. The status of how various operators work with active safety systems and how the systems are implemented in current and future vehicles has been investigated through extensive literature studies, interviews and market research. The autonomous systems can collect information from the surrounding through sensors and contribute to better traffic efficiency, increased safety, lighter cars and a better environment. Through vehicle communication, the vehicle can communicate with each other in order to guarantee a safe ride. In 2030 the inner city constitutes of autonomous, electrified public transport to transport people on demand, meanwhile private cars are prohibited. Potential needs for the human in a fully, autonomous car has been identified and various product development methods has been applied in order to develop two conceptual solutions for a future car interior. The solutions show the interaction between human and system since entertainment and comfort becomes important in a fully, autonomous car. Each solution is state-owned and holds four passengers. In the solutions, the seats are placed in regard to facilitate communication between the passengers. The passengers can be entertained or informed individually or collectively by text, sound and images.

Chimeric antigen receptors (CARs) are artificial molecules composed of an antibody derived antigen recognition domain which is fused with the signal transduction domain derived from the physiological TCR. CAR technology used to transduce patients T-cells and endow them with the specificity to a certain surface antigen, has been a major breakthrough in cancer immunotherapy in the last decade. This strategy has been most successful for treating hematologic malignancies. Various CAR approaches and applications are currently tested mainly in the United States where many clinical trials have been launched. In contrast, in the Czech Republic, there are only a few teams focused on this topic with no clinical trials going on. During my work on this diploma thesis and in close collaboration with MUDr. Pavel Otáhal, PhD., who is working on implementation of CAR technology into the Czech clinics for the treatment of B-cell malignancies, individual functional CARs were prepared and tested. CAR expressing Jurkat T-cell lines were generated using a lentiviral vector transduction system. CAR functionality was determined by two different assays. We have shown that individual CARs are able to recognize the B-cell lineage specific antigens CD19 and CD20 and significantly up-regulate the activation molecule CD69 upon...

L'adoption récente et généralisée des systèmes d'automatisation des véhicules, avec l’incorporation de la connectivité entre voitures, a encouragé l’utilisation des techniques comme le Contrôle Croisière Adaptatif Coopératif (CACC) et la conduite en convoi. Ces techniques ont prouvé l’amélioration du flux de trafic et la sécurité de la conduite, tout en réduisant la consommation d’énergie et les émissions CO_2. Néanmoins, la robustesse et la stabilité stricte du convoi, malgré les délais de communication et l’hétérogénéité des convois, restent des sujets de recherche en cours. Cette thèse a pour sujet la conception, l’analyse et validation de systèmes de contrôle pour le car-following automatisé et coopératif, en ciblant l’augmentation de ses avantages et son usage, en se concentrant sur la robustesse et la stabilité du convoi même sur des séries de véhicules hétérogènes avec des retards de communication. Une structure feedforward/feedback est développée, dont sa modularité est fondamentale pour la mise au point des approches avec des objectifs différents mais complémentaires. L’architecture permet non seulement l’adoption d’une stratégie d’espacement pour la range entière de vitesse, mais elle peut aussi être employée dans le cadre d’un CACC basé sur une machine d’état pour la conduite en convoi sur des environnements urbains avec des capacités de freinage d’urgence et de rejoint du convoi. Des différents algorithmes pour la conception de systèmes de contrôle feedback pour la régulation des distances sont présentés, pour quoi le calcul d’ordre fractionnaire démontre fournir des réponses fréquentielles de boucle fermé plus précises et satisfaire des besoins plus exigeantes. La performance est assurée malgré l’hétérogénéité avec la proposition de deux approches feedforward différents. Le premier est basé sur une topologie en considérant que le véhicule précédent dans la boucle, tandis que le deuxième inclut le véhicule leader pour améliorer la performance de suivi. Les algorithmes proposés sont validés avec des études de stabilité dans le domaine du temps et fréquence, ainsi que simulations et expérimentations réelles. / Recent widespread adoption of vehicle automation and introduction of vehicle-to-vehicle connectivity has opened the doors for techniques as Cooperative Adaptive Cruise Control (CACC) and platooning, showing promising results in terms of traffic capacity and safety improvement, while reducing fuel consumption and CO_2 emissions. However, robustness and strict string stability, despite communication delays and string heterogeneity is still an on-going research field. This thesis deals with the design, study and validation of control systems for cooperative automated car-following, with the purpose of extending their benefits and encourage their employment, focusing on robustness and string stability, despite possible V2V communication delays and string heterogeneity. A feedforward/feedback hierarchical control structure is developed, which modularity is fundamental for the proposal of approaches that target different but complementary performance objectives. The architecture not only permits the adoption of a full speed range spacing policy that target multiple criteria, but can also be employed in a state machine-based CACC framework for urban environments with emergency braking and platoon re-joining capabilities in case of pedestrian interaction. Different feedback control design algorithms are presented for the gap-regulation, for which the fractional-order calculus is demonstrated to provide more accurate closed loop frequency responses and satisfy more demanding requirements. Desired performance is ensured in spite of string heterogeneity through the proposal of two feedforward methods : one based on predecessor-only topology, while the second includes the leader vehicle information on feedforward to gain tracking capabilities. Proposed control algorithms are validated through time and frequency-domain stability studies, simulation and real platforms experiments.

O adequado deslocamento autônomo de robôs móveis entre fileiras de cultura agrícola implica a apropriada configuração estrutural do veículo, bem como considerar a detecção das filas de plantas ou árvores, e também o desenvolvimento de um sistema de controle de locomoção. Esta tese apresenta o desenvolvimento de um sistema de controle em malha fechada baseado na técnica de otimização H∞, que é aplicado no deslocamento entre fileiras de plantio de um robô móvel agrícola em escala reduzida. Mais especificamente, o foco deste trabalho é o seguimento de caminhos na cultura através da aplicação de técnicas de controle robusto. Duas questões foram fundamentais na elaboração da tese: 1) \"Quais são os métodos e procedimentos necessários para implementar a navegação autônoma de um protótipo de robô móvel entre fileiras de cultura agrícola?\" e, 2) \"É possível aplicar os conceitos relativos a sistemas de controle em malha fechada para solucionar o problema da navegação autônoma do robôs móveis entre fileiras de cultura agrícola?\". Primeiramente é apresentada uma revisão bibliográfica sobre robôs móveis agrícolas que tem locomoção baseada em rodas. Posteriormente, os conceitos relacionados com o projeto de controle baseado na técnica de otimização H∞ são fundamentados. Em seguida, são descritos os detalhes relacionados com a construção da plataforma robótica proposta, o projeto do controlador de caminho, as respectivas simulações e as especificações para a realização de testes em ambiente agrícola. Finalmente os resultados alcançados são apresentados. Conclui-se que o sistema de controle proposto se mostrou efetivo na realização da navegação autônoma do robô entre as fileiras da cultura previamente configuradas para a avaliação do seu desempenho. / Appropriate autonomous navigation of mobile robots between crop rows implies, besides appropriate structural configuration, considering detection of plants or trees in rows, as well as the development of a locomotion control system. Consequently, this thesis presents the development of a closed loop control system based on H∞ optimization technique, which is applied to control the navigation of a low scale car-like mobile robot between crop rows; more specifically, main focus of this work is tracking paths in the culture, by the application of robust control techniques. Two questions were fundamental in the development of the thesis: 1) which are the methods and procedures to implement the autonomous navigation of a mobile robot prototype between crop rows? And 2) is it possible to apply the concepts of closed-loop control systems to solve the problem of autonomous navigation of mobile robots between crop rows? Firstly, we provide a literature review on agricultural mobile robots whose mobility depends on wheels. Secondly, control systems design fundamentals based on the H∞ optimization technique are addressed. Thirdly, details related to the construction of the proposed robotic platform and also the design of the proposed path controller (including its simulation and specifications for testing within an agricultural environment) are described. Finally, results of our findings are presented. It is concluded that our control system showed to be effective in the realization of autonomous navigation between crop rows in agricultural environment, which was properly configured in order to evaluate the performance of our robot.