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Att kolhydratuppladda är att fylla kroppens muskler med energi, vilken används för att patienten ska klara av kroppens stressrespons i samband med fasta och operation. Kliniskt får patienten dricka näringsdryck innan operation för att kolhydratuppladda. Inför ett kirurgiskt ingrepp genomgår patienten en förändring i vardagen, lämnar hemmet och kommer till en ovan miljö. Att patienten genomgår fasta och upplever en ny situation påverkar patientens välbefinnande negativt, med både hunger, törst och oro och så vidare. I denna litteraturstudie kommer tio artiklar att granskas för att undersöka, effekterna få en preoperativ kolhydratuppladdning fokus i studien ligger på omvårdnad och välbefinnande men den tar även upp effekter som inte är direkt relaterade till omvårdnadsämnet men knyter an till vården. Studien visar, att under rätt förutsättningar hjälper en preoperativ kolhydratuppladdning att minska till exempel preoperativ törst, hunger och oro. Andra positiva effekter som kan ses i studien är mindre postoperativa kräkningar och bättre bibehållen muskelfunktion. Studien är upplagd i tre olika resultatområden primära resultat, sekundära resultat och tertiära resultat. De olika resultatområdena tar upp olika resultat med olika mycket anknytning till omvårdnad. / A carbohydrate-load is essential to charge the muscles of the body with energy so that the patient will be able to withstand the stress-responses of the body due to fasting and operation. This is clinically facilitated by having the patient ingest a nutritional drink in order to load up on carbohydrates. Preparing for a surgical procedure is a significant change of routine for the patient as he/she leaves its usual environment for an unfamiliar one. Having the patient go through fasting and experience a new situation, will affect the patient’s well-being negatively due to hunger, thirst and mental discomfort etc. Ten articles will be included in this literary study in order to study the effects of a pre-operative carbohydrate load. Focus will be put on the care and well-being of the patient, but also effects that are not directly related to nursing sciences, but in patient care in general. The study will show that under the right circumstances a pre-operative carbohydrate build up will reduce pre-operative thirst, hunger, and discomfort. Other beneficial effects learned from the case study are less post-operative vomiting, and improvement in sustained muscle functions. The results from the case study are presented in the categories of primary, secondary and tertiary results, which will be presented with varying relevance to nursing sciences.

The economics of microalgal bioproduct commercialization would benefit from increased accumulation of energy storage compounds, such as carbohydrates and lipids, and increased biomass productivity. This thesis explores two adaptive laboratory evolution strategies for improving Scenedesmus obliquus: single UV-mutagenesis and low light cultivation at a high dilution rate to produce cultigens with greater carbohydrate content and iterative UV-mutagenesis and selection under outdoor conditions in a raceway pond at a high dilution rate to increase biomass productivity. Two cultigens were generated with the goal of increased carbohydrate content: K5 and K7. Both were mutagenized for 5 seconds and cultivated in 50-mL Klavins reactors. K5 was maintained at 95 μmol/m2-sec for 16 hours/day, and K7 was maintained at 250 μmol/m2-sec for 6 hours/day. When evaluating the two cultigens in triplicate 800-mL bubble columns against wild-type Scenedesmus obliquus, neither K5 nor K7 demonstrated an increase in carbohydrate content. To increase biomass productivity, Scenedesmus obliquus was iteratively UV-mutagenized (aliquots exposed for 5-60 seconds were combined) and cultivated in a 100-L, 0.5-m2 outdoor raceway pond fed with filtered reclaimed wastewater at a high dilution rate. Three rounds of mutagenesis and selection took place during the spring, summer, and winter months, with the MBE 509 and MBE 510 cultigens being produced after the summer and winter selection rounds, respectively. When evaluated in triplicate 800-mL bubble column reactors: MBE 509 (491 ± 42 mg/L-day, expressed as the mean of the time series ± SD) was 24% more productive than wild-type (397 ± 39 mg/L-day), and MBE 510 (443 ± 26 mg/L-day) was 13% more productive than wild-type (391 ± 9 mg/L-day). When evaluated in replicate 1,350-L, 4.5-m2 raceway ponds, MBE 509 (15.2 ± 2.2 g/m2-day) and MBE 510 (16.1 ± 1.8 g/m2-day) were 5% and 12% more productive than wild-type (14.5 ± 2.4 g/m2-day), respectively. Selection under low light and high dilution did not result in cultigens with higher carbohydrate content relative to wild-type Scenedesmus obliquus, but iterative mutagenesis and selection in an outdoor raceway pond did generate cultigens with higher biomass productivity in both indoor and outdoor environments.

Part I . We explored the synthesis of a C -glycoside synthesis from L-fucose and malononitrile in the presence of a base catalyst. The reaction was much faster than the previously studied Henry condensation, and went further---to a double cyclization of 2:1 adduct with a novel dioxabicyclic structure. It provides a new route for the synthesis of chiral polysubstituted dihydropyrans and dihydropurans. A series of carbasugars was synthesized and tested for inhibitory activity towards fungal glycosidases from Aspergillus oryzae and Penicillium canescens . In order to reveal the dependence of inhibition on the alkyl group R, several derivatives with variable alkyl chain lengths were prepared and screened. Part II . Cyclohexane-based conformationally controlled ionophores, an emerging new class of molecular switches, provide a new and promising approach to allosteric systems with negative cooperativity. Protonation of trans -2-aminocyclohexanols was observed to cause dramatic conformational changes: due to an intramolecular hydrogen bond, a conformer with equatorial position of ammonio- and hydroxy-groups becomes predominant. This signal is mechanically transmitted by the structure of the molecule, inducing a conformational change in the second site and decreasing its affinity for an appropriate guest. Thus, these structures can serve as powerful conformational pH-triggers. The trans -2-aminocyclohexanol moiety has been used for pH-triggered conformational switching of crown ethers and podands, and their complexes with metal ions. The variation of the NR 2 group allows broad tuning of the conformational equilibrium, and thus tuning of the complexing ability of these allosteric ionophores. Heterotropic allosteric systems with high negative cooperativity may find many applications, such as membrane transport and drug delivery.

Soil microbiomes play pivotal roles to the health of the environment by maintaining metabolic cycles. One question is how will climate change affect soil bacteria over time and what could the repercussions be. To answer these questions, the Harvard Forest Long-Term Warming Experiment was established to mimic predicted climate change by warming plots of land 5℃ above ambient conditions. In 2017, 14 soil core samples were collected from Barre Woods warming experiment to mark 15 years since the establishment of the soil warming in that location. These samples underwent traditional metatranscriptomics to generate an mRNA library as well as a process coined cell-sorted or mini-metagenomics involving the sorting of single bacterial cells from the environment using FACS. This was followed by pooling into groups of 100 cells for more cost efficient genome recovery. 200 high-quality genomes were compiled, 12 of which were taxonomically identified as Acidobacteria. Acidobacteria are an extremely abundant and diverse phylum of bacteria that were found to be very well represented in the soil samples. Due to their abundance in many different soil environments as well as their known importance in many metabolic cycles, they were chosen as the candidate phylum to further investigate. Using a reference-based read mapping approach with the 12 Acidobacteria genomes and metatranscriptomic data, we identified over 3,000 differentially expressed genes within these organisms as a result of soil warming. Due to the diversity within the phylum itself, many of the genomes indicated different patterns of expression making it difficult to identify phylum-wide differential expression trends. However, the sigma70 factor, an important housekeeping gene used as a transcription regulator, was found to be up-regulated in a majority of the genomes. Over 30 different glycoside hydrolase encoding genes and glycosyltransferases were also found to be differentially expressed across the Acidobacteria reference genomes as well as 23 chemotaxis-related genes. Despite identifying four different groups of genes that showed statistically significant differences in expression levels, there may be more changes occurring in these soil bacteria and the soil microbiome as a whole due to climate change than previously measured by read-based analyses of metatranscriptomic data.

A field and laboratory study was made to determine the effect of alternate year rest rotation grazin in stem bases and root crowns of crested wheatgrass. Analyses for carbohydrate reserves and total nitrogen were made for the following treatments: (1) exclosures; (2) open range; (3) agronomy cages. Both carbohydrate concentration and total nitrogen content showed differences between sampling dates but did not show differences as a result of grazing treatment on individual dates. Differences between sampling dates were associated with season and growth stage of plants. Although differences due to grazing teatment were not shown for individual dates the combined average carbohydrate concentration for plants rested or protected from grazing for one season was higher than from protected plants inside exclosures or from plants grazed during the study. Despite the short duration of this study these results indicate that alternate year rest rotation grazing as practiced on Diamond Mountain is not adversely affecting storage of food reserves in crested wheatgrass.

京都大学 / 新制・論文博士 / 博士(農学) / 乙第13520号 / 論農博第2908号 / 新制||農||1096(附属図書館) / 学位論文||R4||N5420(農学部図書室) / 京都府立医科大学 / (主査)教授 中﨑 鉄也, 教授 土井 元章, 教授 及川 彰, 准教授 田中 朋之 / 学位規則第4条第2項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

Ketohexokinase (KHK), in an adenosine triphosphate (ATP) dependent reaction, catalyzes the first reaction in fructose metabolism, which converts the furanose form of D-fructose into fructose-1-phosphate. This enzyme has become a target for pharmacological development against fatty liver and metabolic syndrome. KHK exists in two isoforms, A and C, which differs by alternative splicing of exon 3 which encodes 45 out of 298 amino acids. Normally KHK exists as a homodimer and is comprised of an alpha/beta domain interlocking with a β-clasp domain. For KHK-C, there appear to be at least two conformations of the β-clasp domain. Previous work on KHK-A reveals it does not adopt the same conformations. A structure of the mouse KHK-A in its unliganded form is solved and shows that these two conformations also exist for KHK-A. Furthermore, this property is conserved across species. While crystals of human KHK-A in its unliganded form were grown, a structure was not achieved. However, unpublished structures of human KHK-A in its unliganded form also shows different conformations in β-clasp domain when in juxtaposition with the same enzyme complex with ligands. Defining the role of conformational changes in KHK-A is important, because this isozyme has been reported to have a role in cancer metastasis.

Background: Type 2 diabetes mellitus is one of the leading chronic diseases affecting Americans. There is a lack of literature discussing the link between diet and prognosis of those already diagnosed with DM. Objective: To provide insight into which diet is better for the outlook of diabetes mellitus by examining the associations between the low-carbohydrate diet (LCD) score and three diabetes-related health indicators: blood hemoglobin (HbA1c), triglycerides, and retinopathy. Methods: A total of 3,313 U.S. adults with DM were selected from the National Health and Nutrition Examination Survey 2005-2016. Presence of retinopathy was ascertained through self-reporting. Dietary intake was measured with 24- hour dietary recalls, and LCD scores were calculated from the proportion of energy of three macronutrients. Scores ranged from 0-30, with a higher score indicating lower carbohydrate intake. Results: There was no significant difference in HbA1c levels between the highest and the lowest quintile of LCD score (mean = 7.42% [95% CI: 7.23, 7.61] vs. 7.32% [95% CI: 7.13, 7.51]). There was no significant association between blood triglyceride levels and LCD score, comparing quintile 1 to quintile 5 (mean= 168.64 mg/dl; 95% CI = [150.14, 187.14] vs. mean= 162.44 mg/dl; 95% CI = [143.76, 181.11]). In multivariable logistic regression analysis, the odds ratio of having retinopathy comparing the highest to the lowest quintile was 1.01 (95% CI: 0.59, 1.72). Conclusion: Proportion of carbohydrate in diet was not associated with DM prognosis factors. Future studies should focus on carbohydrate quality as well as quantity.

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