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Molecular characterization of the fepA-fes bidirectional promoter in escherichia coliMorris, Terry Lynn, January 2001 (has links)
Thesis (Ph. D.)--University of Missouri--Columbia, 2001. / Typescript. Vita. Includes bibliographical references (leaves 135-149). Also available on the Internet.
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Performance Analysis of Diversity Techniques for Wireless Communication SystemISLAM, MD. JAHERUL January 2012 (has links)
Different diversity techniques such as Maximal-Ratio Combining (MRC), Equal-Gain Combining (EGC) and Selection Combining (SC) are described and analyzed. Two branches (N=2) diversity systems that are used for pre-detection combining have been investigated and computed. The statistics of carrier to noise ratio (CNR) and carrier to interference ratio (CIR) without diversity assuming Rayleigh fading model have been examined and then measured for diversity systems. The probability of error (p_e) vs CNR and (p_e) versus CIR have also been obtained. The fading dynamic range of the instantaneous CNR and CIR is reduced remarkably when diversity systems are used [1]. For a certain average probability of error, a higher valued average CNR and CIR is in need for non-diversity systems [1]. But a smaller valued of CNR and CIR are compared to diversity systems. The overall conclusion is that maximal-ratio combining (MRC) achieves the best performance improvement compared to other combining methods. Diversity techniques are very useful to improve the performance of high speed wireless channel to transmit data and information. The problems which considered in this thesis are not new but I have tried to organize, prove and analyze in new ways.
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Acyl-acyl carrier protein synthetases from bluegreen algae and plants / Acyl-Acyl Carrier Protein Synthetasen aus Blaualgen und PflanzenKaczmarzyk, Danuta 29 April 2008 (has links)
No description available.
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Expression of the formin Daam 1 in pyramidal neurons of the hippocampus affects spine morphologySalomon, Steven. January 2006 (has links)
Formins, also known as formin homology (FH) proteins, are involved in a wide range of actin-mediated processes. The Diaphanous-related formin Daam1 (Dishevelled-associated activator of morphogenesis) interacts with the PDZ domain protein Dishevelled, and is required to establish planar cell polarity in Xenopus. Through a yeast two-hybrid screen, I characterized a PDZ-mediated interaction between the C-terminus of Daam1 and the PDZ domains 456 of GRIP1. In dissociated rat hippocampal cultures, Daam1 expression was seen throughout the soma and dendrites in a punctate pattern. Furthermore, co-staining with a synaptic marker suggests that Daam1 could be associated with post-synaptic specializations. Dendritic spines are enriched with actin filaments, and based on the subcellular localization of Daam1 and the evidence that formins are involved in regulating actin polymerization, I hypothesized that Daam1 might play a role in dendritic spine morphology. In order to investigate the functional roles for Daam1, viral vectors were developed using the Semliki-Forest defective viral vector to over-express the full-length Daam1 protein and a Daam1 lacking the PDZ-binding motif. The over-expression of the full-length Daam1 in organotypic hippocampal slices showed a punctate distribution throughout the dendritic shaft, with the occasional appearance in spines, resulting in an overall increase in dendritic spine length. This suggests that formins, such as Daam1, could potentially regulate spine morphology.
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Equalization Algorithms And Performance Analysis In Cyclic-Prefixed Single Carrier And Multicarrier Wireless SystemsItankar, Yogendra Umesh 01 1900 (has links) (PDF)
The work reported in this thesis is divided in to two parts.
In the first part, we report a closed-form bit error rate (BER) performance analysis of orthogonal frequency division multiple access (OFDMA) on the uplink in the presence of carrier frequency offsets (CFOs) and/or timing offsets (TOs) of other users with respect to a desired user. We derive BER expressions using probability density function (pdf) and characteristic function approaches, for a Rician faded multi-cluster multi-path channel model that is typical of indoor ultrawideband channels and underwater acoustic channels. Numerical and simulation results show that the BER expressions derived accurately quantify the performance degradation due to non-zero CFOs and TOs.
Ultrawideband channels in indoor/industrial environment and underwater acoustic channels are severely delay-spread channels, where the number of multipath components can be of the order of tens to hundreds. In the second part of the thesis, we report low complexity equalization algorithms for cyclic-prefixed single carrier (CPSC)systems that operate on such inter-symbol interference(ISI) channels characterized by large delay spreads. Both single-input single-output and multiple-input multiple-output(MIMO) systems are considered. For these systems, we propose a low complexity graph based equalization carried out in frequency domain. Because of the noise whitening effect that happens for large frame sizes and delay spreads in the frequency domain processing, improved performance compared to time domain processing is shown to be achieved. Since the graph based equalizer is a soft-input soft-output equalizer, iterative techniques(turbo-equalization) between detection and decoding are shown to yield good coded BER performance at low complexities in convolutional and LDPC coded systems. We also study joint decoding of LDPC code and equalization of MIMO-ISI channels using a joint factor graph.
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Block Transmissions On Orthogonal CarriersYazici, Ayhan 01 September 2005 (has links) (PDF)
Orthogonal Frequency Division Multiplexing (OFDM) and Single Carrier
Block Transmissions (SCBT) are located at the two opposite edges of block
transmission concept. In this thesis a system which lies between OFDM
and SCBT is proposed. The new system, namely Block Transmissions on
Orthogonal Carriers (BTOC), can be considered as a hybrid form of OFDM
and SCBT. BTOC system is investigated under the redundant filterbank
precoders and equalizers framework. Peak to average power ratio (PAPR) of
BTOC is formulated and compared with the PAPRs of OFDM and SCBT.
Effect of frequency offset for BTOC is investigated and comparison between
OFDM, SCBT, and BTOC is presented. Simulation results of Zero Padded
OFDM (ZP-OFDM), SCBT, and BTOC are included.
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Caractérisation de l'état oligomérique du transporteur mitochondrial ADP/ATP dans des membranes natives / Probing the oligomeric organization of the mitochondrial ATP/ADP carrier in native membranes.Moiseeva, Vera 12 June 2012 (has links)
Le passage sélectif de molécules à travers la membrane interne des mitochondries est essentiel aux processus métaboliques des cellules eucaryotes. Cette communication cellulaire est assurée par des protéines transmembranaires de la famille des transporteurs mitochondriaux (MCF). Le transporteur ADP/ATP (AAC) est le membre le plus connu et le mieux caractérisé de cette famille. Il est responsable de l'import d'ADP dans la matrice mitochondriale et de l'export d'ATP après synthèse vers le cytosol. La structure d'AAC est connue mais plusieurs questions restent ouvertes concernant le mécanisme du transport, la sélectivité et l'état oligomérique, controversé, de la protéine. Pendant plusieurs années des études biochimiques réalisées sur la protéine solubilisée en détergent étaient en faveur d'une organisation dimérique du transporteur, mais la structure d'AAC, monomérique a remis en cause ce dogme. Afin de caractériser l'organisation oligomérique d'AAC in vivo, nous avons combiné plusieurs approches. Nous avons réalisé des expériences de FRET (Fluorescence Resonance Energy Transfer) directement sur des cellules mammifères ou bactériennes (E. coli) surexprimant la protéine AAC fusionnée avec des sondes FRET. En parallèle, nous avons mis au point des tests fonctionnels afin de contrôler l'état des mitochondries et l'activité du transporteur dans ces cellules. Enfin nous avons étudié la stoechiométrie de liaison de l'inhibiteur carboxyatractyloside grâce à des mesures de respiration sur des mitochondries extraites de foie de rat et placées dans différents états métaboliques. L'ensemble des résultats présentés dans ce manuscrit ont permis de montrer que 1) l'unité fonctionnelle d'AAC est monomérique 2) l'organisation structurale d'AAC dans les membranes natives dépend de l'état métabolique des mitochondries et peut être associée à des phénomènes de régulation. / The transport of small molecules through the inner mitochondrial membrane is essential in eukaryotic metabolism and is selectively controlled by a family of integral membrane proteins, the Mitochondrial Carrier Family (MCF). The ADP/ATP carrier (AAC), which is responsible for the import of ADP to the matrix of mitochondria and the export of newly synthesized ATP toward the cytosol, is the best-known and characterized MCF member. Although its structure sheds light on several aspects of the carrier activity, additional investigations are still required to decipher the whole transport mechanism, to understand the specificity and to characterize the controversial oligomeric state of the protein. For many years, based on studies mainly carried on detergent solubilized AAC the general consensus has been in favor of a dimeric organization of the carrier. The AAC three-dimensional structure, monomeric, broke this dogma. In order to get a precise insight into the in vivo oligomeric organization of AAC we combined several approaches. Fluorescence resonance energy transfer (FRET) measurements were performed directly on mammalian and E.coli cells expressing AAC labeled with several types of FRET probes. In parallel, different functional assays were established to control the state of the mitochondria in these cells and the transport activity of these AAC fusions. Lastly, measurements of the respiration rate coupled to the titration of the inhibitory effect of carboxyatractyloside on isolated rat liver mitochondria were used to investigate the organization of AAC in native mitochondria within two regimes of oxidative phosphorylation. Taken together the results described herein revealed that 1) AAC can function mechanistically as a monomer, 2) the organization of AAC in native membranes might be related to the state of the mitochondria and be involved in regulation.
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Impact of carrier localization on recombination in InGaN quantum wells with nonbasal crystallographic orientationsIvanov, Ruslan January 2017 (has links)
The modern InGaN technology demonstrates high efficiencies only in the blue spectral region and low current operation modes. The growth of InGaN quantum wells (QWs) on nonbasal crystallographic planes (NBP) has potential to deliver high-power blue and green light emitting diodes and lasers. The emission properties of these QWs are largely determined by the localization of carriers in the minima of spatially inhomogeneous band potential, which affects the recombination dynamics, spectral characteristics of the emission, its optical polarization and carrier transport. Understanding it is crucial for increasing the efficiency of NBP structures to their theoretical limit. In this thesis, the influence of carrier localization on the critical aspects of light emission has been investigated in semipolar and nonpolar InGaN QWs. For this purpose, novel multimode scanning near-field optical microscopy configurations have been developed, allowing mapping of the spectrally-, time-, and polarization-resolved emission. In the nonpolar QW structures the sub-micrometer band gap fluctuations could be assigned to the selective incorporation of indium on different slopes of the undulations, while in the smoother semipolar QWs – to the nonuniformity of QW growth. The nanoscale band potential fluctuations and the carrier localization were found to increase with increasing indium percentage in the InGaN alloy. In spite to the large depth of the potential minima, the localized valence band states were found to retain properties of the corresponding bands. The reduced carrier transfer between localization sites has been suggested as a reason for the long recombination times in the green-emitting semipolar QWs. Sharp increase of the radiative lifetimes has been assigned to the effect of nanoscale electric fields resulting from nonplanar QW interfaces. Lastly, the ambipolar carrier diffusion has been measured, revealing ~100 nm diffusion length and high anisotropy. / <p>QC 20170919</p>
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Density of States and Charge Carrier Transport in Organic Donor-Acceptor Blend Layers / Zustandsdichte und Ladungsträgertransport in Organischen Donator-Akzeptor-MischschichtenFischer, Janine 23 October 2015 (has links) (PDF)
In the last 25 years, organic or "plastic" solar cells have gained commercial interest as a light-weight, flexible, colorful, and potentially low-cost technology for direct solar energy conversion into electrical power. Currently, organic solar cells with a maximum power conversion effciency (PCE) of 12% can compete with classical silicon technology under certain conditions. In particular, a variety of strongly absorbing organic molecules is available, enabling custom-built organic solar cells for versatile applications. In order to improve the PCE, the charge carrier mobility in organic thin films must be improved. The transport characterization of the relevant materials is usually done in neat layers for simplicity. However, the active layer of highly efficient organic solar cells comprises a bulk heterojunction (BHJ) of a donor and an acceptor component necessary for effective charge carrier generation from photo-generated excitons.
In the literature, the transport properties of such blend layers are hardly studied. In this work, the transport properties of typical BHJ layers are investigated using space-charge limited currents (SCLC), conductivity, impedance spectroscopy (IS), and thermally stimulated currents (TSC) in order to model the transport with numerical drift-diffusion simulations. Firstly, the influence of an exponential density of trap states on the thickness dependence of SCLCs in devices with Ohmic injection contacts is investigated by simulations. Then, the results are applied to SCLC and conductivity measurements of electron- and hole-only devices of ZnPc:C60 at different mixing ratios. Particularly, the field and charge carrier density dependence of the mobility is evaluated, suggesting that the hole transport is dominated by exponential tail states acting as trapping sites. For comparison, transport in DCV5T-Me33:C60, which shows better PCEs in solar cells, is shown not to be dominated by traps.
Furthermore, a temperature-dependent IS analysis of weakly p-doped ZnPc:C60 (1:1) blend reveals the energy-resolved distribution of occupied states, containing a Gaussian trap state as well as exponential tail states. The obtained results can be considered a basis for the characterization of trap states in organic solar cells. Moreover, the precise knowledge of the transport-relevant trap states is shown to facilitate modeling of complete devices, constituting a basis for predictive simulations of optimized device structures. / Organische oder "Plastik"-Solarzellen haben in den letzten 25 Jahren eine rasante Entwicklung durchlaufen. Kommerziell sind sie vor allem wegen ihres geringen Gewichts, Biegsamkeit, Farbigkeit und potentiell geringen Herstellungskosten interessant, was zukünftig auf spezielle Anwendungen zugeschnittene Solarzellen ermöglichen wird. Die Leistungseffzienz von 12% ist dabei unter günstigen Bedingungen bereits mit klassischer Siliziumtechnologie konkurrenzfähig. Um die Effzienz weiter zu steigern und damit die Wirtschaftlichkeit zu erhöhen, muss vor allem die Ladungsträgerbeweglichkeit verbessert werden. In organischen Solarzellen werden typischerweise Donator-Akzeptor-Mischschichten verwendet, die für die effziente Generation freier Ladungsträger aus photo-induzierten Exzitonen verantwortlich sind. Obwohl solche Mischschichten typisch für organische Solarzellen sind, werden Transportuntersuchungen der relevanten Materialien der Einfachheit halber meist in ungemischten Schichten durchgeführt.
In der vorliegenden Arbeit wird der Ladungstransport in Donator-Akzeptor-Mischschichten mithilfe raumladungsbegrenzter Ströme (space-charge limited currents, SCLCs), Leitfähigkeit, Impedanzspektroskopie (IS) und thermisch-generierter Ströme (thermally stimulated currents, TSC) untersucht und mit numerischen Drift-Diffusions-Simulationen modelliert. Zunächst wird mittels Simulation der Einfluss exponentiell verteilter Fallenzustände auf das schichtdickenabhängige SCLC-Verhalten unipolarer Bauelemente mit Ohmschen Kontakten untersucht. Die Erkenntnisse werden dann auf Elektronen- und Lochtransport in ZnPc:C60-Mischschichten mit verschiedenen Mischverhältnissen angewendet. Dabei wird die Beweglichkeit als Funktion von elektrischem Feld und Ladungsträgerdichte dargestellt, um SCLC- und Leitfähigkeitsmessungen zu erklären, was mit einer exponentiellen Fallenverteilung gelingt.
Zum Vergleich werden dieselben Untersuchungen in DCV2-5T-Me33:C60, dem effizientesten der bekannten Solarzellenmaterialien dieser Art, wiederholt, ohne Anzeichen für fallendominierten Transport. Des weiteren werden erstmals schwach p-dotierte ZnPc:C60-Mischschichten mit temperaturabhängiger IS untersucht, um direkt die Dichte besetzter Lochfallenzustände zu bestimmen. Dabei werden wiederum exponentielle Fallenzustände sowie eine Gaußförmige Falle beobachtet. Insgesamt tragen die über Fallenzustände in Mischschichten gewonnenen Erkenntnisse zum Verständnis von Transportprozessen bei und bilden damit eine Grundlage für die systematische Identifizierung von Fallenzuständen in Solarzellen. Außerdem wird gezeigt, dass die genaue Beschreibung der transportrelevanten Fallenzustände die Modellierung von Bauelementen ermöglicht, auf deren Grundlage zukünftig optimierte Probenstrukturen vorhergesagt werden können.
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Aviation industry in global perspective / Letecký průmysl v globální perspektivěVeselý, Martin January 2015 (has links)
The hypothesis claims that full service carriers (FSCs) will be forced to change their operating models. Additionally, the trends which form aviation market of the future are explored. The investigation is based on a financial assessment of ten important airlines incorporated in four different regions across the globe, between 2005 and 2014. According to the findings the trend of liberalization will continue, thus FSCs will continue losing market share to low cost carriers (LCCs) and as such, they will be made to change the way they operate in order to survive. The future aviation market is defined by a reshuffle of demand towards emerging countries, further rationalization of operating models and consolidation.
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