Carcinoma de Células Renais (CCR) representa uma família de tumores distintos com evolução clínica imprevisível. Uma variedade de moléculas tem sido avaliada como marcadores prognósticos para CCR. Ciclina D1, uma proteína reguladora do ciclo celular, encontra-se superexpressa em vários tumores primários. Nosso objetivo é avaliar sua expressão como marcador prognóstico em CCR. Antes disso, traçamos um perfil clínico e histopatológico da amostra e verificamos sua relação com os fatores prognósticos considerados clássicos pela literatura. 109 espécimes de pacientes diagnosticados com CCR foram obtidos entre 2005 e 2010 no Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto USP e submetidos à análise imunoistoquímica juntamente com 07 amostras de tecido renal normal. A maior parte das características epidemiológicas e clínicas de nossa amostra foi similar àquelas descritas na literatura mundial. Houve predomínio do gênero masculino, da raça branca, com idade próxima a 60 anos, frequência de pacientes assintomáticos em torno de 36% e grande prevalência do CCR de células claras (71,55%). A mortalidade específica da doença foi de 13,76%, sendo o CCR de células claras o tipo mais frequente entre os óbitos e casos metastáticos. Os casos que exibiram má evolução clínica, definida pela ocorrência de metástase e/ou óbito por CCR (22,01%), estiveram associados à presença de sintomas ao diagnóstico, maior tamanho tumoral, grupo de estágio alto (III ou IV), grau nuclear de Fuhrman alto (3 ou 4), presença de necrose e de diferenciação sarcomatóide no tumor, além de outros fatores histológicos desfavoráveis (p < 0,01). Isso indica que as variáveis utilizadas na avaliação de prognóstico em países desenvolvidos podem ser aplicadas aos nossos pacientes. Não houve expressão imunoistoquímica de Ciclina D1 nos casos de tecido renal normal. Observou-se heterogeneidade de marcação nuclear intratumoral no total de casos e menor expressão proteica entre os CCR papilífero e cromófobo. Pacientes com tumores com Ciclina D1baixa (até 30% de células positivas) apresentaram má evolução clínica (p = 0,03), maior tamanho tumoral (p = 0,01), presença de sintomas ao diagnóstico (p = 0,04), grau nuclear alto (p = 0,001), presença de necrose (p = 0,004) e de diferenciação sarcomatóide (p = 0,04) no tumor, além de menor sobrevida sem metástase e/ou óbito por CCR (p = 0,03). Após análise multivariada, a expressão de Ciclina D1 não apresentou valor prognóstico independente para má evolução clínica, embora tenha aumentado levemente a acurácia prognóstica do modelo adotado. Em todas as análises realizadas para o CCR de células claras isoladamente, observamos significância estatística semelhante à do total de casos (CCR). Nosso estudo demonstrou que: a proteína Ciclina D1 encontra-se superexpressa em CCR; os tipos de CCR parecem exibir diferentes padrões de marcação imunoistoquímica da Ciclina D1; alta marcação da proteína (acima de 30% de células positivas) esteve associada à boa evolução clínica e à maioria dos fatores prognósticos favoráveis bem estabelecidos na literatura. Novas investigações são necessárias para descobrir que mecanismos levam a seu acúmulo nas células neoplásicas e quais outros eventos podem estar contribuindo para a progressão da doença. / Renal Cell Carcinoma (RCC) is a family of distinct tumors with unpredictable clinical outcome. A variety of molecules have been evaluated as prognostic markers for RCC. Cyclin D1, a cell cycle regulatory protein, is overexpressed in several primary tumors. Our purpose is to evaluate its expression as a prognostic marker in RCC. Before that, we drew a clinical and histopathological profile of the sample and verified its relationship with prognostic factors regarded as classics in literature. 109 specimens from patients diagnosed with RCC were obtained between 2005 and 2010 at Hospital das Clínicas - Ribeirão Preto School of Medicine USP and submitted to immunohistochemical analysis, along with 07 normal kidney tissue samples. Most epidemiological and clinical characteristics of our sample were similar to those described in the literature. There was a predominance of male, Caucasian, aged about 60 years, the frequency of asymptomatic patients around 36%, and high prevalence of clear cell RCC (71.55%). The disease-specific mortality was 13.76%, being the clear cell RCC the most frequent type among deaths and metastatic cases. Cases that exhibited poor clinical outcome, defined by the occurrence of metastasis and/or death by RCC (22.01%), were related to the presence of symptoms at diagnosis, larger tumor size, high stage group (III or IV), high Fuhrman nuclear grade (3 or 4), presence of necrosis and sarcomatoid differentiation in the tumor and other unfavorable histological factors (p < 0.01). This indicates that the variables used in the assessment of prognosis in developed countries can be applied to our patients. There was no immunohistochemical expression of Cyclin D1 in cases of normal kidney tissue. There was intratumoral heterogeneity in nuclear staining in all cases and lower protein expression among papillary and chromophobe RCC. Patients with Cyclin D1low tumors (up to 30% positive cells) showed poor clinical outcome (p = 0.03), larger tumor size (p = 0.01), presence of symptoms at diagnosis (p = 0.04), high nuclear grade (p = 0.001), presence of necrosis (p = 0.004) and sarcomatoid differentiation (p = 0.04) in the tumor and lower survival without metastasis and/or death by RCC (p = 0.03). After multivariate analysis, the expression of Cyclin D1 showed no independent prognostic value for poor clinical outcome, although it has slightly increased the prognostic accuracy of the model adopted. In all analyzes performed for clear cell RCC alone, we observed statistical significance similar to that of the total cases (RCC). Our study showed that: Cyclin D1 protein is overexpressed in RCC; RCC types seem to exhibit different patterns of immunohistochemical staining for Cyclin D1; high protein expression (over 30% positive cells) was related to good clinical outcome and to most favorable prognostic factors well established in the literature. Further investigations are necessary to reveal which mechanisms lead to its accumulation in neoplastic cells and what other events might be contributing to the progression of the disease.

A expansão das áreas de reflorestamento colocam em evidência os possíveis efeitos destas nos recursos hídricos. Tendo como principal característica o crescimento acelerado, as florestas plantadas apresentam elevadas taxas de evapotranspiração, e, embora não sejam consideradas usuárias de água, o consumo das mesmas modifica a produção de água na microbacia. Buscando entender este cenário, o objetivo do estudo foi identificar alternativas de corte raso em mosaico a serem utilizadas para mitigar os efeitos das florestas plantadas nos recursos hídricos. Foram testados variáveis de intensidade e configuração espacial do corte raso e seus efeitos obtidos por meio de simulações chuva-vazão na microbacia experimental de Itatinga. O estudo foi dividido em duas partes, a primeira foi referente à análise do consumo por meio da curva de permanência do deflúvio em diferentes cenários de intensidade de corte, e a segunda pela simulação hidrológica distribuída de cenários de corte variáveis tanto na intensidade quanto na localização. A partir dos resultados obtidos foram realizadas comparações com as vazões mínimas de referência (Q7.10 e Q90) para quantificação do consumo de água das florestas ao longo do tempo. Os resultados mostraram que quando totalmente coberta com floresta o consumo de água sempre ficava além do recomendado, conforme o corte da cobertura simulado era intensificado (até 100% de corte) os valores de consumo caiam, sendo que a partir de cinquenta porcento de corte o deflúvio ficava abaixo dos dois índices de vazão mínima de referência analisados. Na segunda parte do estudo os cenários de intensidade e localização do corte (30, 50 e 70% de corte nas cotas baixas e altas) foram comparados com a referência - cobertura total com floresta - e os resultados analisados quanto ao escoamento total, pico de vazão e volume dos eventos. Foi também simulado um cenário para a análise do efeito da vegetação ripária na redução dos impactos. Os resultados mostraram que os efeitos das diferentes intensidades de corte raso estão relacionados a altura total precipitada, pois apresentaram diferenças significativas para os menores eventos de chuva, não tendo as intensidades diferenças no corte para os maiores eventos (lâminas de chuva acima de 15 mm). A localização do corte nas cotas baixas da bacia resultou em picos de vazão e volume maiores do que o cenário referência para uma mesma intensidade de corte. Quando os cortes foram realizados nas cotas altas os resultados não diferiram significativamente do cenário referência em nenhuma porcentagem de corte, com exceção para a altura precipitada entre 4 e 8 mm. As simulações dos efeitos da floresta ripária mostraram que quando mantida ao longo do rio a vegetação diminui o escoamento direto, no entanto, este efeito depende da intensidade do evento de chuva, não sendo significativo para grandes eventos. Por meio dos dados foi possível concluir que o corte em mosaico é uma ferramenta que deve ser utilizada no manejo das florestas plantadas, pois por meio deste é possível diminuir o consumo através da intensidade e mitigar os efeitos do aumento do escoamento por meio da localização do corte. / The expansion of areas of planted forests shows possible effects they have on water resources. Due to the characteristics of accelerated growth, commercial reforestations have high rates of evapotranspiration. Although planted forests are not considered water users, their consumption modifies water production in the watershed. Seeking to understand this scenario, the objective of this study was to identify alternatives to clear-cutting in mosaic that can be used to mitigate the effects of planted forests in water resources. Variables of intensity and spatial configuration of clear-cutting and its effects obtained through rain-flow simulations were tested in the experimental watershed of Itatinga. The study was divided into two parts, the first consisted of the analysis of consumption through the flow permanence curve in different scenarios of cut intensity, and the second was characterized by hydrological simulation of distributed variable cutting scenarios both in intensity and in location. The results were used to compare with the minimum flow rates of reference (Q7.10 and Q90) to quantify water consumption of forests over time, measuring the relationship between consumption and minimum reference flow. The results showed that when fully covered with forest, water consumption was always beyond the recommended, and as the simulated cut of the vegetation cover was enhanced (until 100% cutting), consumption values dropped. After 50 percent of cutting, runoff remained below two rates of minimum flow reference analyzed. In the second part of the study, the scenarios of intensity and location of cuts (30, 50 and 70% of cut in downstream and upstream) were compared with reference - complete forest cover - and the results were analyzed in terms of total runoff, peak flow and volume of events. It was also simulated a scenario for the analysis of the effects of riparian vegetation on the reduction of impacts caused by the clear-cutting of the planted forest. The effects of different intensities of clear-cutting are related to the size of the event of rain, because they showed significant differences for smaller rain events, however, no differences in the intensities were observed for greater events (precipitation above 15 mm). The location of the cut in downstream scenarios resulted at peak flow and volume larger than the reference scenario for the same cut intensity. When the cuts were made in upstream, the results did not differ significantly from the reference scenario in any percentage of cut, except for the rain size between 4 and 8 mm. Simulations of the effects of riparian forest showed that when they are kept along the river, the vegetation decreases the direct runoff, however, this effect depends on the intensity of the rain event, not being significant for large events. The use of data allowed to conclude that the cut in mosaic is a tool that could be used in the management of planted forests, as it decreases consumption through intensity and mitigates the effects of increased runoff due to the location of the cut.

Made available in DSpace on 2016-04-25T19:20:55Z (GMT). No. of bitstreams: 1 Julia Signer e Cruz.pdf: 940902 bytes, checksum: 08ea98e2b16c5857fa67dab7a4a23bab (MD5) Previous issue date: 2008-09-15 / This paper has as purpose to observe the psychological effects of the rigpa´s (clear light) experience among some Tibetan Buddhist practioners. As I didn t find academic researches with this object, this paper came to fullfill this fault. We use as theoretical base the Jung s psychology and the philosophy of Ati and Anutara Yogas of Tibetan Buddhism. Twenty two practioners, ages between 23 and 71 years old of both sexes were interviewed. The results allows us to conclude that rigpa s experience make possible to realize the human existence in a more open way where the Consciousness is fixed in a place deeper than the ego, what may produce a existential experience of union with the universe, a natural feeling of joy, freedom and well being, a body relaxation and a sense of mental openness with a spontaneously sense of natural wisdom / Esta dissertação tem como objetivo observar os efeitos psicológicos da experiência de rigpa (clara luz) entre praticantes do Budismo Tibetano. Como não foram encontradas pesquisas acadêmicas que enfoquem esse objeto, o presente estudo visa suprir essa carência. Como base teórica usamos a psicologia junguiana e a filosofia das yogas Ati e Anutara do Budismo Tibetano. Realizou-se uma pesquisa de campo constituída de entrevistas semi-abertas com 22 praticantes de idades variantes entre 23 e 71 anos, de ambos os sexos. Os resultados revelam que a experiência de rigpa permite conceber a existência humana de maneira mais profunda e sediar a Consciência em um lugar mais profundo do que o eu, o que leva a uma vivência existencial unida a todo o Universo, a um sentimento espontâneo de alegria, liberdade e leveza, a um relaxamento corporal e a uma sensação de abertura mental, com o surgimento espontâneo de uma sabedoria natural

Le syndrome von Hippel Lindau (VHL) prédispose au développement de multiples tumeurs hautement vascularisées, telles que des hémangioblastomes rétiniens ou du système nerveux central, des phéochromocytomes et des carcinomes rénaux à cellules claires (CCRCC). Les patients atteints de ce syndrome sont porteurs d’une mutation du gène VHL. Ce gène, composé de trois exons, est transcrit en deux ARN messagers par épissage alternatif de l’exon 2. L’ARNm composé des 3 exons (variant #1) est la forme majoritairement exprimée par rapport à l’ARNm dépourvu de l’exon 2 (variant #2). Toutefois, une diminution du ratio variant #1/variant #2 a été essentiellement décrite dans deux situations : (i) dans les tissus embryonnaires humains et en particulier le rein, et (ii) dans certains CCRCC. Ces données suggèrent un rôle potentiel de ce variant #2 dans la tumorigenèse rénale. Deux protéines, pVHL213 et pVHL160, sont produites à partir du variant #1 et elles agissent comme suppresseurs de tumeur. Au début de ce travail, l’expression de l’isoforme protéique pVHL172 produite à partir du variant #2 restait à démontrer et sa fonction était inconnue. Les travaux effectués au cours de cette thèse ont permis de mettre en évidence l’expression de pVHL172 dans des lignées cellulaires et dans des tissus tumoraux grâce à un nouvel anticorps monoclonal de souris dirigé contre les trois isoformes protéiques humaines de pVHL. Pour savoir si l’isoforme pVHL172 a un rôle de suppresseur de tumeur, des lignées cellulaires tumorales rénales exprimant stablement cette protéine ont été établies puis des expériences de xénogreffes de ces cellules chez la souris ont été réalisées. Non seulement pVHL172 n’inhibe pas la formation de tumeurs mais son expression induit un phénotype tumoral plus agressif avec une composante sarcomatoïde plus importante ainsi qu’une vascularisation immature plus conséquente que dans les tumeurs contrôles (n’exprimant pas pVHL). De plus, pVHL172 augmente l’expression des métalloprotéases de matrice MMP1 et MMP13, en partie via l’activation de la voie de signalisation Smad-dépendante du TGF-β. Par ailleurs, des partenaires protéiques de cette protéine ont été recherchés par une analyse protéomique différentielle. Les réseaux d’interaction réalisés à partir des protéines identifiées concernent entre autres la régulation de la matrice extracellulaire et le contrôle qualité des protéines. En conclusion, ce travail a montré que le gène VHL produit des isoformes protéiques avec des fonctions distinctes voire antagonistes, ce qui implique que la balance de leur expression influencerait la progression tumorale rénale. Chez certains patients, une augmentation de l’expression de pVHL172 pourrait être corrélée à une pathologie plus sévère. Ce travail montre l’intérêt de poursuivre l’étude des fonctions de cette protéine pour une meilleure compréhension de son implication dans le cancer du rein et dans la maladie VHL afin d’envisager de nouvelles approches thérapeutiques. / VHL disease predisposes to the development of multiple and highly vascularized tumors, including central nervous system and retinal haemangioblastomas, phaeochromocytomas and clear cell renal cell carcinomas (ccRCCs). Patients with VHL disease harbor a mutant allele of the VHL gene. This gene is transcribed into two mRNAs by alternative splicing of the exon 2. The mRNA variant #1 composed of 3 exons usually predominates over the mRNA variant #2 lacking exon 2. A decrease of the variant #1/variant #2 ratio was however described in 2 situations: (i) in embryonic tissues, particularly in the kidney, and (ii) in some ccRCCs. These data suggest a potential role for the variant #2 in kidney tumorigenesis. pVHL213 and pVHL160 are the two proteins encoded by the mRNA variant #1 and act as tumor suppressors. At the beginning of this Ph.D. project, the expression of pVHL172 isoform encoded by the mRNA variant #2 remained to be established and its function was unknown. The experiments performed during this Ph.D. shed light on pVHL172 expression in cell lines and in tumor tissues using a newly produced mouse monoclonal antibody recognizing the three human pVHL isoforms. To examine if pVHL172 had a tumor suppressor function, human kidney tumor cell lines stably expressing this isoform were established, characterized and then grafted in mice. pVHL172 not only inhibits tumor formation, but its expression also induces a more aggressive phenotype with a higher sarcomatoid component and a more immature vasculature compared to control tumors (that do not express any pVHL). Moreover, pVHL172 increases the matrix metalloproteases MMP1 and MMP13 expression, partly by the activation of the Smad-dependent TGF-β signalling pathway. Besides, we looked for protein partners of pVHL172 by a differential proteomic analysis and showed that interaction networks obtained with the identified proteins are related to extracellular matrix regulation and protein quality control. To conclude, this work demonstrated that the VHL gene encodes protein isoforms with distinct and even antagonistic functions. The balance of expression of these isoforms is likely to influence kidney tumor progression. For some patients, an increase of pVHL172 expression could be correlated with a more severe pathology. This work shows the importance of further studying this isoform’s functions to better understand its involvement in kidney cancer and in VHL disease, so that new therapeutic approaches could be developed.

Carcinoma de Células Renais (CCR) representa uma família de tumores distintos com evolução clínica imprevisível. Uma variedade de moléculas tem sido avaliada como marcadores prognósticos para CCR. Ciclina D1, uma proteína reguladora do ciclo celular, encontra-se superexpressa em vários tumores primários. Nosso objetivo é avaliar sua expressão como marcador prognóstico em CCR. Antes disso, traçamos um perfil clínico e histopatológico da amostra e verificamos sua relação com os fatores prognósticos considerados clássicos pela literatura. 109 espécimes de pacientes diagnosticados com CCR foram obtidos entre 2005 e 2010 no Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto USP e submetidos à análise imunoistoquímica juntamente com 07 amostras de tecido renal normal. A maior parte das características epidemiológicas e clínicas de nossa amostra foi similar àquelas descritas na literatura mundial. Houve predomínio do gênero masculino, da raça branca, com idade próxima a 60 anos, frequência de pacientes assintomáticos em torno de 36% e grande prevalência do CCR de células claras (71,55%). A mortalidade específica da doença foi de 13,76%, sendo o CCR de células claras o tipo mais frequente entre os óbitos e casos metastáticos. Os casos que exibiram má evolução clínica, definida pela ocorrência de metástase e/ou óbito por CCR (22,01%), estiveram associados à presença de sintomas ao diagnóstico, maior tamanho tumoral, grupo de estágio alto (III ou IV), grau nuclear de Fuhrman alto (3 ou 4), presença de necrose e de diferenciação sarcomatóide no tumor, além de outros fatores histológicos desfavoráveis (p < 0,01). Isso indica que as variáveis utilizadas na avaliação de prognóstico em países desenvolvidos podem ser aplicadas aos nossos pacientes. Não houve expressão imunoistoquímica de Ciclina D1 nos casos de tecido renal normal. Observou-se heterogeneidade de marcação nuclear intratumoral no total de casos e menor expressão proteica entre os CCR papilífero e cromófobo. Pacientes com tumores com Ciclina D1baixa (até 30% de células positivas) apresentaram má evolução clínica (p = 0,03), maior tamanho tumoral (p = 0,01), presença de sintomas ao diagnóstico (p = 0,04), grau nuclear alto (p = 0,001), presença de necrose (p = 0,004) e de diferenciação sarcomatóide (p = 0,04) no tumor, além de menor sobrevida sem metástase e/ou óbito por CCR (p = 0,03). Após análise multivariada, a expressão de Ciclina D1 não apresentou valor prognóstico independente para má evolução clínica, embora tenha aumentado levemente a acurácia prognóstica do modelo adotado. Em todas as análises realizadas para o CCR de células claras isoladamente, observamos significância estatística semelhante à do total de casos (CCR). Nosso estudo demonstrou que: a proteína Ciclina D1 encontra-se superexpressa em CCR; os tipos de CCR parecem exibir diferentes padrões de marcação imunoistoquímica da Ciclina D1; alta marcação da proteína (acima de 30% de células positivas) esteve associada à boa evolução clínica e à maioria dos fatores prognósticos favoráveis bem estabelecidos na literatura. Novas investigações são necessárias para descobrir que mecanismos levam a seu acúmulo nas células neoplásicas e quais outros eventos podem estar contribuindo para a progressão da doença. / Renal Cell Carcinoma (RCC) is a family of distinct tumors with unpredictable clinical outcome. A variety of molecules have been evaluated as prognostic markers for RCC. Cyclin D1, a cell cycle regulatory protein, is overexpressed in several primary tumors. Our purpose is to evaluate its expression as a prognostic marker in RCC. Before that, we drew a clinical and histopathological profile of the sample and verified its relationship with prognostic factors regarded as classics in literature. 109 specimens from patients diagnosed with RCC were obtained between 2005 and 2010 at Hospital das Clínicas - Ribeirão Preto School of Medicine USP and submitted to immunohistochemical analysis, along with 07 normal kidney tissue samples. Most epidemiological and clinical characteristics of our sample were similar to those described in the literature. There was a predominance of male, Caucasian, aged about 60 years, the frequency of asymptomatic patients around 36%, and high prevalence of clear cell RCC (71.55%). The disease-specific mortality was 13.76%, being the clear cell RCC the most frequent type among deaths and metastatic cases. Cases that exhibited poor clinical outcome, defined by the occurrence of metastasis and/or death by RCC (22.01%), were related to the presence of symptoms at diagnosis, larger tumor size, high stage group (III or IV), high Fuhrman nuclear grade (3 or 4), presence of necrosis and sarcomatoid differentiation in the tumor and other unfavorable histological factors (p < 0.01). This indicates that the variables used in the assessment of prognosis in developed countries can be applied to our patients. There was no immunohistochemical expression of Cyclin D1 in cases of normal kidney tissue. There was intratumoral heterogeneity in nuclear staining in all cases and lower protein expression among papillary and chromophobe RCC. Patients with Cyclin D1low tumors (up to 30% positive cells) showed poor clinical outcome (p = 0.03), larger tumor size (p = 0.01), presence of symptoms at diagnosis (p = 0.04), high nuclear grade (p = 0.001), presence of necrosis (p = 0.004) and sarcomatoid differentiation (p = 0.04) in the tumor and lower survival without metastasis and/or death by RCC (p = 0.03). After multivariate analysis, the expression of Cyclin D1 showed no independent prognostic value for poor clinical outcome, although it has slightly increased the prognostic accuracy of the model adopted. In all analyzes performed for clear cell RCC alone, we observed statistical significance similar to that of the total cases (RCC). Our study showed that: Cyclin D1 protein is overexpressed in RCC; RCC types seem to exhibit different patterns of immunohistochemical staining for Cyclin D1; high protein expression (over 30% positive cells) was related to good clinical outcome and to most favorable prognostic factors well established in the literature. Further investigations are necessary to reveal which mechanisms lead to its accumulation in neoplastic cells and what other events might be contributing to the progression of the disease.

Submitted by Fernanda Ciolfi (fernanda.ciolfi@puc-campinas.edu.br) on 2016-08-11T16:55:41Z No. of bitstreams: 1 Angelica Dayane Sena de Oliveira Lima.pdf: 2802577 bytes, checksum: fc91a553c7b4a91077d0d3479f459333 (MD5) / Made available in DSpace on 2016-08-11T16:55:41Z (GMT). No. of bitstreams: 1 Angelica Dayane Sena de Oliveira Lima.pdf: 2802577 bytes, checksum: fc91a553c7b4a91077d0d3479f459333 (MD5) Previous issue date: 2016-06-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This paper deals the open spaces system in the city of Campinas, through research of environmental laws at the federal and municipal level, analyzing in sequence the proposals set out by the Director and Plan Local Plans of city management in relation to areas permanent preservation. For this took place a temporal reading of the legislative content of the Forest Code, the Constitution, the National Environmental Council and current Director Plan of Campinas during the study period. some theoretical concepts were used as: space, free space, free space system, public space, linear park, as reading instruments and understanding of the work object and the studied regions. To identify the consequences of environmental legislation in the urban space, and the possible configuration of a system of environmental character of open spaces in Campinas, were chosen three macro-areas (3, 5 and 8) to be analyzed in this work. Them were discussed propositions of linear parks defined by Management Plans Sites; the morphological aspects of these spaces; the relationship between these spaces and the urban fabric and, finally, sought to identify when and where the propositions of linear parks were to be prioritized and executed, and when these proposals could be spaces reserved for preservation, without losing sight of, the possibility of the future, be carried out as public spaces for leisure. / O presente trabalho trata sobre o sistema de espa?os livres da cidade de Campinas, atrav?s da investiga??o das leis ambientais, na esfera federal e municipal, analisando em sequ?ncia as proposi??es definidas pelo Plano Diretor e pelos Planos Locais de Gest?o da cidade, com rela??o ?s ?reas de preserva??o permanente. Para isto realizou-se uma leitura temporal do conte?do legislativo do C?digo Florestal, Constitui??o Federal, Conselho Nacional do Meio Ambiente e Plano Diretor de Campinas vigente no per?odo da pesquisa. Foram utilizados alguns conceitos te?ricos como: espa?o, espa?o livre, sistema de espa?os livres, espa?o p?blico, parque linear, como instrumentos de leitura e compreens?o do objeto de trabalho e das regi?es estudadas. Para identificar os desdobramentos da legisla??o ambiental no espa?o urbano, e a poss?vel configura??o de um sistema de espa?os livres de car?ter ambiental em Campinas, foram escolhidas tr?s macrozonas (3, 5 e 8) a serem analisadas neste trabalho. Nelas foram discutidas as proposi??es de parques lineares definidos pelos Planos Locais de Gest?o; os aspectos morfol?gicos desses espa?os; a rela??o entre esses espa?os e o tecido urbano e, por ?ltimo, procurou identificar quando e onde as proposi??es de parques lineares deveriam ser priorizadas e executadas, e quando essas propostas poderiam ser espa?os reservados ? preserva??o, sem perder de vista, a possibilidade de no futuro, serem realizadas enquanto espa?os p?blicos de lazer.

A expansão das áreas de reflorestamento colocam em evidência os possíveis efeitos destas nos recursos hídricos. Tendo como principal característica o crescimento acelerado, as florestas plantadas apresentam elevadas taxas de evapotranspiração, e, embora não sejam consideradas usuárias de água, o consumo das mesmas modifica a produção de água na microbacia. Buscando entender este cenário, o objetivo do estudo foi identificar alternativas de corte raso em mosaico a serem utilizadas para mitigar os efeitos das florestas plantadas nos recursos hídricos. Foram testados variáveis de intensidade e configuração espacial do corte raso e seus efeitos obtidos por meio de simulações chuva-vazão na microbacia experimental de Itatinga. O estudo foi dividido em duas partes, a primeira foi referente à análise do consumo por meio da curva de permanência do deflúvio em diferentes cenários de intensidade de corte, e a segunda pela simulação hidrológica distribuída de cenários de corte variáveis tanto na intensidade quanto na localização. A partir dos resultados obtidos foram realizadas comparações com as vazões mínimas de referência (Q7.10 e Q90) para quantificação do consumo de água das florestas ao longo do tempo. Os resultados mostraram que quando totalmente coberta com floresta o consumo de água sempre ficava além do recomendado, conforme o corte da cobertura simulado era intensificado (até 100% de corte) os valores de consumo caiam, sendo que a partir de cinquenta porcento de corte o deflúvio ficava abaixo dos dois índices de vazão mínima de referência analisados. Na segunda parte do estudo os cenários de intensidade e localização do corte (30, 50 e 70% de corte nas cotas baixas e altas) foram comparados com a referência - cobertura total com floresta - e os resultados analisados quanto ao escoamento total, pico de vazão e volume dos eventos. Foi também simulado um cenário para a análise do efeito da vegetação ripária na redução dos impactos. Os resultados mostraram que os efeitos das diferentes intensidades de corte raso estão relacionados a altura total precipitada, pois apresentaram diferenças significativas para os menores eventos de chuva, não tendo as intensidades diferenças no corte para os maiores eventos (lâminas de chuva acima de 15 mm). A localização do corte nas cotas baixas da bacia resultou em picos de vazão e volume maiores do que o cenário referência para uma mesma intensidade de corte. Quando os cortes foram realizados nas cotas altas os resultados não diferiram significativamente do cenário referência em nenhuma porcentagem de corte, com exceção para a altura precipitada entre 4 e 8 mm. As simulações dos efeitos da floresta ripária mostraram que quando mantida ao longo do rio a vegetação diminui o escoamento direto, no entanto, este efeito depende da intensidade do evento de chuva, não sendo significativo para grandes eventos. Por meio dos dados foi possível concluir que o corte em mosaico é uma ferramenta que deve ser utilizada no manejo das florestas plantadas, pois por meio deste é possível diminuir o consumo através da intensidade e mitigar os efeitos do aumento do escoamento por meio da localização do corte. / The expansion of areas of planted forests shows possible effects they have on water resources. Due to the characteristics of accelerated growth, commercial reforestations have high rates of evapotranspiration. Although planted forests are not considered water users, their consumption modifies water production in the watershed. Seeking to understand this scenario, the objective of this study was to identify alternatives to clear-cutting in mosaic that can be used to mitigate the effects of planted forests in water resources. Variables of intensity and spatial configuration of clear-cutting and its effects obtained through rain-flow simulations were tested in the experimental watershed of Itatinga. The study was divided into two parts, the first consisted of the analysis of consumption through the flow permanence curve in different scenarios of cut intensity, and the second was characterized by hydrological simulation of distributed variable cutting scenarios both in intensity and in location. The results were used to compare with the minimum flow rates of reference (Q7.10 and Q90) to quantify water consumption of forests over time, measuring the relationship between consumption and minimum reference flow. The results showed that when fully covered with forest, water consumption was always beyond the recommended, and as the simulated cut of the vegetation cover was enhanced (until 100% cutting), consumption values dropped. After 50 percent of cutting, runoff remained below two rates of minimum flow reference analyzed. In the second part of the study, the scenarios of intensity and location of cuts (30, 50 and 70% of cut in downstream and upstream) were compared with reference - complete forest cover - and the results were analyzed in terms of total runoff, peak flow and volume of events. It was also simulated a scenario for the analysis of the effects of riparian vegetation on the reduction of impacts caused by the clear-cutting of the planted forest. The effects of different intensities of clear-cutting are related to the size of the event of rain, because they showed significant differences for smaller rain events, however, no differences in the intensities were observed for greater events (precipitation above 15 mm). The location of the cut in downstream scenarios resulted at peak flow and volume larger than the reference scenario for the same cut intensity. When the cuts were made in upstream, the results did not differ significantly from the reference scenario in any percentage of cut, except for the rain size between 4 and 8 mm. Simulations of the effects of riparian forest showed that when they are kept along the river, the vegetation decreases the direct runoff, however, this effect depends on the intensity of the rain event, not being significant for large events. The use of data allowed to conclude that the cut in mosaic is a tool that could be used in the management of planted forests, as it decreases consumption through intensity and mitigates the effects of increased runoff due to the location of the cut.

On étudie l'abstraction et la généricité dans les langages de programmation. On décrit les principaux outils de la programmation générique. On traite ensuite de la spécification des types et des fonctions et on étudie les methodes de vérification de programmes spécifiés algébriquement.

<p>There has been a long-term decline in number of cyclic vole populations in boreal Sweden since the 1970s. Several hypotheses have been suggested to explain this decline. Commonly for <i>C. glareolus</i>, <i>C. rufocanus</i> and <i>M. agrestis</i>, the decline has followed upon an increased frequency and severeness of winter declines and has shown up as a drop in spring densities. The spring decline is most pronounced for <i>C. rufocanus</i>. In contrast to other voles, <i>C. rufocanus</i> also show a decline in fall densities, suggesting some additional disturbance in this species. Habitat fragmentation has been suggested as such an additional disturbance and in this thesis the effect of habitat fragmentation on <i>C. rufocanus</i> is explored.</p><p>At first the sampling method was evaluated i.e. whether the decline could be due to destructive sampling when the method in use in the long-term monitoring is snap-trapping. This resulted in a rejection of the destructive sampling hypothesis as a possible cause behind the decline in <i>C. rufocanus</i>. Habitat preference revealed that three habitats at the local scale (trap station) were high quality habitats for <i>C. rufocanus</i>: forest of moist and wet/hydric dwarf-shrub type, in addition to forest/swamp complexes rich in dwarf-shrubs. The occurrence of <i>C. rufocanus</i> at the landscape scale was positively correlated with the amount of boulder fields and a low degree of fragmentation of old-growth pine forests. There was considerable local variation in the decline in vole density among the 58 1-ha sampling plots, with respect to both density and timing of the decline, which suggested that habitat destruction outside sampling plots might be involved. Overall, clear-cuts had a negative influence on vole densities at both the local and landscape scale. A multiple regression analysis suggested that having both a high quality habitat at the local scale and a high proximity among xeric-mesic mires and a low connectivity among clear-cuts at the landscape scale were important for the occurrence of <i>C. rufocanus</i>.</p><p>Initial analysis at the landscape scale were based on landscape data collected from 2.5 x 2.5 km areas centred on the individual vole sampling plots. Further investigations, however, on the patch level suggest that focal forest patch size and quality was of major importance in determining occurrence and persistence of <i>C. rufocanus</i>. Although not tested formally in these studies, the habitat fragmentation hypothesis has so far received support. Currently <i>C. rufocanus</i> seems to be affected negatively by too low patch sizes of suitable habitats in the surrounding landscape suggesting that the amount of suitable habitats could already be below the fragmentation threshold. However, this has to be evaluated further. Work is in progress to establish time-series over local landscape changes, and to evaluate if such changes have been associated with local declines of <i>C. rufocanus</i> and whether habitat loss, true habitat fragmentation or both have been influential.</p>

In this thesis, fluid flow and solute transport in heterogeneous aquifers and particularly in frac-tured rock have been investigated using Lagrangian Stochastic Advective-Reaction (LaSAR) framework. The heterogeneity of the aquifer structure or fracture configuration, as well as the various reaction/retention processes have been considered in the modelling approach. Advection and retention processes are considered to be the dominant transport processes. Monte-Carlo simulation results for transport of nonreactive tracers in 2D generic heterogeneous aquifers indicate that the travel time τ can be well approximated by a lognormal distribution up to a relative high degree of heterogeneity of the aquifers. Comparison between the Monte-Carlo simulation results and the results of first-order approximation reveals that the analytical solutions of the statistical moments of τ are valid only when the variability of the aquifer properties is small. For reactive tracers, Monte-Carlo simulations have been conducted by accounting for spatial variability of both hydraulic conductivity and one sorption parameter simultaneously. The simulation results indicate that the reaction flow path μ is a nonlinear function of distance for shorter distance, linear function for longer distance, and also that μ and τ are well correlated over the considered parameter range. The parameter β, which is purely determined by the flow condi-tions, quantifies the hydrodynamic control of retention processes for transport of tracers in frac-tures. Numerical simulations have been performed to study the statistical properties of the pa-rameter β, travel time τ and flow rate Q in a single heterogeneous fracture and in a sequence of fractures. The results of Monte-Carlo simulations indicate that the parameter β and τ are corre-lated with a power-law relationship β ∼ τm. The correlation between β and the flow rate Q have also been studied and an inverse power-law relationship β ∼ Q-m is proposed. The establishment of these relationships provides a link between the parameter β and measurable parameters τ (or Q). The LaSAR approach has been applied for prediction, evaluation and interpretation of the results of a number of tracer tests (TRUE-1, TRUE Block Scale and TRUE Block Scale Continuation) conducted by SKB at the Äspö site for tracer transport in fractures. The breakthrough curves may be predicted reasonably well, provided that the retention parameters, boundary conditions and hydraulic properties of the domain are given. The evaluation of TRUE tests indicates that the retention occurs mainly in the rim zone on site characterization time scales, while on the per-formance assessment time scale, diffusion and sorption in the unaltered rock matrix are likely to become dominant retention mechanisms. / QC 20100624