While pornography has become highly accessible, another way in which pornography is present in media is through discussion in movies and television shows, which is often framed through casual reference, by way of a joke, or in a teasing manner. A content analysis of 55 episodes that reference pornography—looking specifically at the framing of pornography in individual scenes—provides valuable insight into the media ecosystem surrounding pornography and its potential impact on adolescents. Using cultivation analysis and framing theory as a theoretical basis, this study analyzes the inclusion of the terms, “porn,” “porno,” and “pornography” in popular television shows.

Producing pharmaceuticals in Escherichia coli inevitably comes with an extensive purification process. This is because many of the native proteins of E. coli are immunogenic to humans, especially the heat and pH resistant endotoxins located in the membrane of E. coli. These native proteins drive up the cost of the purification, which led to a request from the biopharmaceutical company Affibody AB. They want a review on the possibilities of producing their unique Affibody®-molecules in a new, less problematic host cell. Based on a previous bachelor project, Affibody AB chose Pichia pastoris as the candidate. P. pastoris is a methylotrophic yeast that is increasing in use when it comes to producing pharmaceuticals. In this review, multiple ways of utilizing P. pastoris are presented. The process proposals are based on 4 different promoters, pAOX1, pAOX2, pFLD1 and the pGAP. The AOX1- and AOX2-promoters and the FLD1-promoter are inducible promoters that require an inducer-molecule. An inducible promoter presents the best control of the process. The GAP-promoter is a constitutive promoter, meaning that the gene is expressed continuously. A constitutive promoter provides a process which requires fewer steps and ingredients. If the Affibody®-molecules were to be produced with P. pastoris as the host cell, the products would contain less immunogenic substances. Further, P. pastoris is also a very effective option when it comes to producing protein extracellularly. This would ultimately lead to a purification process that requires less resources.

Bacterial cultivation from patient samples is the foundation of proper antibiotic treatment. A frequently used cultivation medium is agar with an additive of blood, so-called blood agar. The recommended additive of blood is horse blood while human blood is currently used as an additive at the microbiological laboratory Ryhov, Jönköping. The study aimed to evaluate horse blood in blood agar prior to a change in blood from human to horse blood according to a regulation from the European Union. The method consisted of cultivation from reference strains, clinical isolations and clinical patient samples on both blood agar with human blood and blood agar with horse blood. The comparison included a visual assessment for both blood agar based on the criteria of haemolysis, colony size and bacterial growth. In the assessment of clinical patient samples, in addition to the previously mentioned criteria, mixed flora was also assessed. The study resulted in a more distinct demonstration of haemolysis on blood agar with horse blood than on blood agar with human blood for haemolytic bacteria. An equivalence for both blood agar was presented regarding the remaining criteria. Based on these results a change can be made according to the regulation without negative impact.

Microalgae have received a surge in attention in the past decade as a source for renewable energy. They are currently used to produce high-value products but have the potential to produce biofuels such as bioethanol, biodiesel and biohydrogen among others. The present work is an evaluation of the feasibility of a process producing microalgae as a wet biomass paste through modeling of a real process. The production process of microalgal-derived low-value products has yet to be realized due to the low productivity in current culture systems, the high cost of such systems and the high cost of harvesting the microalgal biomass. An accurate and efficient model of this process is key in allowing for scale up to where it can contribute sufficiently to a more sustainable society. The model constructed evaluates the best alternative out of twelve configurations using data supplied by the user. The model is flexible in that it allows for modeling of different microalgae and different scales of production. Genetic engineering of the microalgae is modeled as three different cases: a reduction in the light-harvesting antennae, the expression of a fluorescent protein that by absorbing UV light emits photosynthetically active radiation and a combination of the two. The model is then run for the case relevant to the user. To evaluate the performance of the model and the viability of the modeled process, it was ran for the microalgae P. tricornutum and an annual production demand of 1000 ton wet biomass paste. The break-even price of the biomass was 8.79 Ä for the base case and 5.68 Ä with both cases of genetic engineering of the microalgal cell. The best configuration was found to be a tubular photobioreactor in the cultivation stage with flocculation-sedimentation followed by filtration in the harvesting stage. The model is capable of producing results that are in line with the current research and are thus deemed reasonable. With the level of accuracy that the model presently contains, it is a good representation of the production of microalgal biomass as a whole. However, to be able to draw definitive conclusions, more time and research is required. / Intresset för mikroalger som en förnyelsebar energikälla har ökat under det senaste decenniet. För närvarande används mikroalger enbart för produktion av specialprodukter men de har potential att användas vid produktion av bland annat bioetanol, biodiesel och vätgas. Detta arbete är en teknoekonomisk studie av produktion av våt biomassa från mikroalger genom modellering av en verklig process. En sådan process finns ännu inte i stor skala på grund av den låga produktiviteten i nuvarande odlingssystem och dess höga kostnader samt de höga kostnaderna kopplade till skördning av biomassan. För att skala upp denna process krävs en noggrann och effektiv modell som simulerar processen samt hur den kan förbättras genom att genetiskt modifiera mikroalgerna. Modellen som konstruerats tar in data från användaren och utvärderar den bästa processen av tolv konfigurationer. Modellen är flexibel och tillåter simulering av olika typer av mikroalger och olika produktionskrav. Genetisk modifiering av mikroalgerna modelleras för tre fall: en reducering av ljuskomplexens antennstorlekar, uttryckning av ett fluorescerande protein som absorberar UV-ljus och emitterar fotosyntetiskt aktiv strålning och en kombination av båda. Modellen körs sedan för det fall som användaren finner relevant, ett basfall utan genetiska modifieringar och med dessa. För att utvärdera modellens prestation och den modellerade processens genomförbarhet kördes den för mikroalgen P. tricornutum och en årlig produktion på 1000 ton våt biomassa. För att uppnå ett nollresultat krävs ett försäljningspris på 8.79 Ä för basfallet och 5.68 Ä för fallet med båda de genetiska modifieringarna. Den bästa konfigurationen var en tubreaktor i odlingssteget följt av flockulering och sedimentering följt av filtrering. Modellen är kapabel till producera resultat som stämmer överens med den nuvarande forskningen inom området och därmed bedöms de som rimliga. Modellen ger i helhet en god representation av produktion av biomassa, men för att dra definitiva slutsatser kring genomförbarheten av denna process så krävs det att mer tid och forskning investeras i att förbättra modellen och dess noggrannhet.

Der vorliegende Bericht stellt Ergebnisse des zweijährigen Projektes 'Veränderte Pflanzenschutzmittel- und Nährstoffausträge bei bodenschonender Bewirtschaftung - Risikoanalyse und -vorsorge' vor. Auf landwirtschaftlichen Flächen in der Lommatzscher Pflege wurden mit einer Beregnungsanlage Starkniederschläge (worst-case-Szenario) simuliert. Die Untersuchungen zu Wasser- und Agrochemikalientransport fanden auf Flächen statt, die konventionell mit Pflug, konservierend flach bearbeitet bzw. in Direktsaat bestellt wurden. Die Auswirkungen auf vertikale und horizontale Stofftransporte, insbesondere von Nitrat-Stickstoff und dem Totalherbizid Durano mit dem Wirkstoff Glyphosat wurden untersucht.

Mit dem vorliegenden Forschungsprojekt wurde das Ziel verfolgt, für den Anbau von Substratkulturen unter den Bedingungen des Freistaates Sachsen wirtschaftlich optimierte Anbaufolgen und - empfehlungen zu erarbeiten. Im Vordergrund der Betrachtungen standen dabei die beiden wichtigsten Fruchtgemüsearten Gurke und Tomate.

In mehrjährigen Stoppelbearbeitungsversuchen mit Grubbern, Scheiben- und Kreiseleggen sollten Einsatzhinweise zur Verringerung der Bodenerosion bei pflugloser Bodenbearbeitung für verschiedene Maschinentypen entwickelt werden. Dazu wurde vor allem die Bodenbedeckung mit Mulchmaterial als wichtiger Indikator für den Schutzgrad beobachtet. Zusätzlich wurden Daten zur Stroheinarbeitung in den Boden sowie zur Ausprägung der Bodenoberflächen und Bearbeitungssohlen ermittelt und der Zugleistungsbedarf für die Geräte gemessen. Im Ergebnis konnte der Bodenbedeckungsgrad nur wenig durch Veränderung der Einsatzbedingungen der Stoppelbearbeitungsmaschinen beeinflusst werden. In erosionsgefährdeten Gebieten kommt daher dem Zwischenfruchtanbau und Bodenbearbeitungsverfahren, die einen hohen Bodenbedeckungsgrad gewährleisten (Streifenbodenbearbeitung, Direktsaat), eine entscheidende Rolle zu. Der Bericht enthält die vollständigen Ergebnisse der Untersuchung.

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This thesis explores the dimensions of an emerging design space in the Anthropocene through a design process that embraces Probology as a method to integrate interdisciplinary research in exploring and implementing solutions to the dissociation of urbanites from Earth’s ecological community.

京都大学 / 0048 / 新制・課程博士 / 博士(地域研究) / 甲第17839号 / 地博第157号 / 新制||地||53(附属図書館) / 30654 / 京都大学大学院アジア・アフリカ地域研究研究科東南アジア地域研究専攻 / (主査)教授 小林 繁男, 教授 河野 泰之, 准教授 竹田 晋也, 准教授 古澤 拓郎 / 学位規則第4条第1項該当 / Doctor of Area Studies / Kyoto University / DFAM