The Swedish National Testing and Research Institute is maintaining the Swedish realization of the world time scale UTC, called UTC(SP). One area of research and development for The Swedish National Laboratory of Time and Frequency is time synchronization and how UTC(SP) can be distributed in Sweden. Dissemination of time information by SP is in Sweden mainly performed via Internet using the Network Time Protocol (NTP) as well as via a modem dial up service and a speaking clock (Fröken Ur). In addition to these services, time information from the Global Positioning System (GPS) and from the long-wave transmitter DCF77 in Germany, is also available in Sweden. This master’s thesis considers how different available commercial communication systems could be used for multicast time distribution. DECT, Bluetooth, Mobile Telecommunication and Radio Broadcasting are different techniques that are investigated. One application of Radio Broadcasting, DARC, was found to be interesting for a more detailed study. A theoretical description of how DARC could be used for national time distribution is accomplished and a practical implementation of a test system is developed to evaluate the possibilities to use DARC for multicast time distribution. The tests of DARC and the radio broadcast system showed that these could be interesting techniques to distribute time with an accuracy of a couple of milliseconds. This quality level is not obtained today but would be possible with some alterations of the system.

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The emergence of wide-bandgap-based (WBG) devices, such as silicon carbide (SiC) and gallium nitride (GaN), have unveiled unprecedented opportunities, enabling the realization of superior power conversion systems. Among the potential areas of advancement are medium-voltage (MV) and high-voltage (HV) applications, due to the growing demand for high-power-density and high-efficiency power electronics converters. These advancements have propelled a wide adoption of electric vehicles (EV), which in the future will require great improvements in the charging time of these vehicles. Thereby, this thesis attempts to address such a challenge and bring about technological improvements, enabling faster, more efficient, and more effective ways of charging an electric vehicle through the application of MV 3.3 kV SiC MOSFETs. The current fast-charging solution involves heavy and bulky MV-LV transformers, which add installation complexity for EV charging stations. However, this thesis presents an alternative power-delivery solution utilizing an MV dual-active-bridge (DAB) converter. The proposed architecture is designed to directly interface with the MV grid for high-power, fast-charging capabilities while eliminating the need for an installation of the MV-LV transformer. The MV DAB converter utilizes 3.3 kV SiC MOSFETs to realize the next 800 V EV charging system, along with an extended zero-voltage-switching (ZVS) scheme, in order to provide an efficient charging strategy across a wide range of battery voltage levels. Lastly, a detailed design comparison analysis of an MV Flyback converter, targeted for the auxiliary power supply for the proposed MV EV charging architecture, is presented. / The field of power electronics, which controls and manages the conversion of electrical energy, is an important topic of discussion, as new technologies like electric vehicles (EV) are quickly emerging and disrupting the current status-quo of vehicle-choice. In order to promote timely and extensive adoption of such an enabling EV technology, it is critical to understand the current challenges involving EV charging stations and seek out opportunities to engender future innovations. Indeed, wide-bandgap (WBG) devices, such as silicon carbide (SiC) and gallium nitride (GaN), have unveiled unprecedented opportunities in enabling the realization of superior power conversion systems. Thus, utilizing these WGB devices in EV charging applications can bring about improved design and development of EV fast chargers that are faster-charging, more efficient, and more effective. Hence, this thesis presents an opportunity in EV charging station applications with the utilization of medium-voltage SiC MOSFETs. Because the current fast-charging solution involves a heavy and bulky transformer, it adds installation complexity for EV charging stations. However, this thesis presents an alternative power-delivery solution that could potentially provide an efficient and fast-charging mechanism of EVs while reducing the size of EV chargers. All things considered, this thesis provides in-depth evaluation-studies of medium-voltage 3.3 kV SiC MOSFET-based power converters, targeted for future fast EV charging applications. The development and design of the hardware prototype is presented in this thesis, along with testing and verification of experimental results.

21世紀是數位化時代，網路傳播、數字電視、電子報刊、各種傳統媒體都進軍數位化市場。在科技的整合下，通訊、資訊、廣播已發展成為三位體，廣播不再是單一節目的音訊，透過數位媒介可以結合多媒體傳輸，DAB數位音訊廣播也演化成為DMB數位多媒體廣播，傳播高品質聲音並能傳輸影像、圖文等，替廣播帶來多元化的服務，數位廣播因此被稱為「看得到的聲音」”Visual Voice”或「豐富化聲音」” Rich Voice”。然而，國內數位廣播產業的發展已瀕臨絕境，業者紛紛棄收，唯一存活業者正奮力企盼找出活路。本研究經由對個案進行系統化分析，找出國外範例成功之關鍵因素，建立可能之營運模式，以供業者參考。 本研究以目前發展最為活絡之韓國DAB/DMB個案：T-DMB陣營與TU Media為探討個案，以波特五力分析找出其成關鍵因素，並藉此建立可能之營運模式與產業鏈。以此為基礎審視國內DAB數位廣播發展現況與唯一仍在運作的寶島新聲數位廣播為個案，找出其定位以及可能之營運模式。 本研究經由波特五力分析並歸納發現，韓國DAB/DMB個案：T-DMB陣營與TU Media成功關鍵因素包括：獨特內容、通路、合理價格、廣播訊號覆蓋率佳以及政策創造良好產業環境。其營運模式為跳脫原先廣播業者的定位，將自己定位為內容聚集商或平台營運商，結盟電信業、內容提供商、通路商、接收機製造商等成為一網絡關係系統，並且藉由電信業已架構完整之商務營運平台進行內容訂閱收費制度，開創新收入來源，廣播業者收入不僅限於廣告費。因之，以本研究之國內個案-寶島新聲數位廣播電台而言，其欲成功推展DAB數位廣播業務須借鏡韓國模式，重新定位，並擴大原網絡系統，與產業上下游相關業者，甚至於跨產業結盟。另一方面，政策也應重新定義數位廣播產業在國內數位化計劃的角色，找出適切之政策，以創造良好之產業環境。 / The 21st century is the digital age, the internet, the digital television, the electronic publication, traditional media is going digital. The “chips” changed out live. Under the digital convergence , the communication, the information, the broadcast have been converged. The DAB/DMB is based on the digital technology, and therefore can converged physically with the digitalized communications infrastructure . The digitalized broadcast are not only the voice programme , also multimedia which transmitting video, jpg files, text etc. Therefore, the DAB is called” Visual Voice” or “ Rich Voice”. However, our DAB broadcast industry is dying , the only operator , TNT DAB Radio Station which is the smallest DAB broadcaster is struggling to survival. In view of this, this research will focus on the South Korea cases :T-DMB league and TU Media which are the success model in the world. This research will analyze the business model, industry value chain, key success factors by Porter 5 force analysis and Competitive Advantage. Base on this, this research tries to find the success way of TNT DAB Radio Station. According to the service model of the T-DMB league and TU Media , this study discover the key success factors including unique content, distribution channels, affordable price, broadcast signal quality coverage and policy creating good industry environment. The T-DMB league and TU Media reposition and play the role of operator in the industry. Also they play the content aggregator. The T-DMB league and TU Media leverage to receiver manufactures, distribution channels and provide “Broadcasting and Communication Convergence Services”. The profit model is the content subscription and advertisement fee . Because of it, TNT DAB Radio Station should re-define the strategy- not broadcaster, should be service operator or content aggregator and build up the alliance. On the other hand, the government also should re-think the broadcast policy and create the good industrial environment.

<p>DAB (Digital Audio Broadcasting) is the radio digital system developed as an european standard by the ETSI, EN 300 400, based on the Eureka-147 group works, to improve the performance of the analogue radio systems (AM and FM). The system is based on the OFDM technology which allows DAB to exploit the spectrum frequencies in a better way with a higher quality of sound for mobile receivers specially. The main part of the OFDM system is based on the FFT algorithms to spread the data flow over different orthogonal carriers. The simulation has been developed in Simulink^TMand Matlab^TMand the layout designed follows faithfully the standard for the transmission system. The simulation can be reloaded by the user with the information presented in this thesis. Thus, this work can be continued to complete the DAB whole system simulation. The results obtained running this simulation show the main DAB system characteristics.</p>

Viruses are submicroscopic obligate parasites and depend on the host cell for their growth and reproduction. Plants are infected by diverse group of viruses that mostly possess RNA as their genome. As exception, viruses belonging to the family Geminiviridae are DNA viruses and infect both mono and dicotyledonous plants causing a large economic loss. These viruses are smaller in size encoding fewer proteins and employ the host cell machinery for successful infection and spread. Geminiviruses undergo frequent recombinations due to mixed infection resulting in vast diversity across the family and account for driving evolution in these viruses. Movement in these viruses is complex since they have to cross two important barriers, nuclear and cell wall barrier to establish systemic spread. All these factors play very important role while designing control measures against these viruses. Thus a detailed understanding of these processes at molecular level is essential. Cotton is the major cash crop in Indian subcontinent with huge export values. India has become the second largest producer of cotton in the world. However, the major constraint in cotton cultivation has been crop loss due to diseases caused by viruses, particularly the cotton leaf curl disease (CLCuD) caused by begomoviruses. Present thesis deals with the analysis of genetic variability of CLCuD in India and functional characterization of proteins involved in the movement of Cotton Leaf Curl Kokhran Virus-Dabawali (CLCuKV-Dab). CLCuKV-Dab belongs to family Geminiviridae and subgroup Begomovirus. A review of the literature on Geminiviridae classification, genome organization, virus entry, replication, transcription, translation, assembly and movement is presented in Chapter 1. This chapter also includes the review of host factors involved in replication, geminiviral proteins involved in gene silencing and a detailed report on CLCuD complexes and sub viral DNAs that are associated with CLCuD. The materials used in this study and the experimental protocols followed such as construction of recombinant clones, their overexpression in both bacterial and baculovirus expression systems, Protein purification techniques, site directed mutagenesis and all other biochemical, molecular biology and cell biology methods are described in detail in Chapter 2. Previous study has reported the complete genomic sequences of CLCuKV-Dab and Tomato leaf curl Bangalore virus-cotton [Fatehabad] (ToLCBV-Cotton [Fat]) and partial sequence of CLCuKV-Gang and the Cotton leaf curl Rajasthan virus (CLCuRV-Ban). Phylogenetic analysis of DNA-A sequences of these viruses with other CLCuD causing viruses is discussed in detail in Chapter 3. Chapter 4 deals with overexpression, purification and functional characterization of CLCuKV-Dab CP in terms of its interaction with DNA, the kinetics and its role in cell to cell movement. The proposed partners to CP in the cell to cell movement of monopartite begomoviruses are AV2 and AC4. Thus the Chapter 5 describes the functional characterization of recombinant AV2 of CLCuKV-Dab. Chapter 6 deals with expression of CP and AV2 as GFP fusion proteins in insect cells using baculovirus expression system to study the localization patterns of these proteins. Chapter 7 describes functional characterization of CLCuKV-Dab AC4. Bioinformatic analysis of AC4 showed that it belongs to the rare group of natively unfolded proteins that are functionally active In conclusion, there is a large genetic variability that exists among the begomoviruses and in particular, among the CLCuD causing begomoviruses in India. Functional characterization of the proteins involved in the cell to cell movement in CLCuKV-Dab led to a possible model for its movement; the CP translated in the cytoplasm is targeted the nucleus via its NLS and there binds to progeny ssDNA and exports the ssDNA out of nucleus through its export signals. AC4 or some other host proteins yet to be identified transports the ssDNA-CP complex from the nuclear periphery to AV2 present at the cell periphery. The complex is then transported from one cell to the neighboring cell via plasmodesmata. AC4 being an ATPase/NTPase could provide energy for the process.

DAB (Digital Audio Broadcasting) is the radio digital system developed as an european standard by the ETSI, EN 300 400, based on the Eureka-147 group works, to improve the performance of the analogue radio systems (AM and FM). The system is based on the OFDM technology which allows DAB to exploit the spectrum frequencies in a better way with a higher quality of sound for mobile receivers specially. The main part of the OFDM system is based on the FFT algorithms to spread the data flow over different orthogonal carriers. The simulation has been developed in Simulink&lt;sup&gt;TM&lt;/sup&gt;and Matlab&lt;sup&gt;TM&lt;/sup&gt;and the layout designed follows faithfully the standard for the transmission system. The simulation can be reloaded by the user with the information presented in this thesis. Thus, this work can be continued to complete the DAB whole system simulation. The results obtained running this simulation show the main DAB system characteristics.

Sicyos deppei G. Don (Cucurbitaceae) is an invasive monoic annual tendril-bearing vine; it is endemic to Mexico, adapted to the rainfall cycle (June through the end of September) and produces spiny, single seeded fruits. Under serious infestation conditions, S. deppei grows extensively, covering native plants, crops and tree trunks. This study determined the breeding system and pollinators of S. deppei growing in three study sites at urban gardens and disturbed sites at the Campus of the Universidad de las Américas, Puebla in the city of San Andrés Cholula, Puebla (Mexico). Each female inflorescence had an average of 17 flowers at the three study sites. As a step prior to experiments, the timing for stigma receptiveness and pollen viability was determined with the Peroxtesmo KO test (PKO) and Diaminobenzidine test (DAB),respectively. Stigmas of female flowers reach maximum receptivity when flowers are at anthesis; receptivity decreases as the flower reaches the succeeding floral stages. Viability of pollen grains increases with flower development; viabilitypercentages at early stages of floral development are very low, as opposed to the higher percentages of pollen viability found in flowers at anthesis and following developmental stages. Breeding systems were tested for xenogamy and geitonogamy by hand pollinating female flowers. Breeding systems results demonstrated that S. i deppei has a mixed-mating system, being able to set fruit and seed when pollinated with pollen from different plants and from pollen of the same plants. The pollinators were determined with the single-visit method. The diversity of floral visitors in this study was low; the most effective pollinator for S. deppei in this study was Apis mellifera. Throughout the rainy season, this study also described the phenology of S. deppei at the plant and flower level, as well as total plant length. Each stage of development in male and female flowers lasts one day. At the end of the rainy season (N2010) individual plants measured between 947 and 270 cm. / CONACYT, NSERC-CANPOLIN