Global ETD Search

11	Thermo-mechanical Analysis of Laser Hot-wire Directed Energy Deposition (LHW-DED) Additive Manufacturing Process Kalel, Mukesh 03 May 2023 (has links) No description available. Mechanical Engineering Materials Science Engineering Aerospace Materials Direct Energy Deposition DED Additive Manufacturing AM Laser Hot wire Direct Energy Deposition LHW-DED Finite Element Modeling FEM Transient-Thermal ANSYS Simulation Transient-Structural Simulation Heat Transfer 3D Printing Simulation Thermal - Deformation Thermal - Distortion Thermal Validation Thermo-Mechanical Modeling
12	Towards Control of Dutch Elm Disease: dsRNAs and the Regulation of Gene Expression in Ophiostoma novo-ulmi / dsRNAs and the Regulation of Gene Expression in Ophiostoma novo-ulmi Carneiro, Joyce Silva 01 August 2013 (has links) Ophiostoma novo-ulmi is the causal agent of Dutch elm disease (DED) which has had a severe impact on the urban landscape in Canada. This research program focused on developing molecular genetic strategies to control this pathogenic fungus. The first strategy involved the development of RNA interference (RNAi) for the down-regulation of genes involved in pathogenicity. An efficient RNAi cassette was developed to suppress the expression of the endopolygalacturonase (epg1) locus which encodes a cell-wall degrading enzyme. This epg1-RNAi cassette significantly reduced the amount of polygalacturonase activity in the fungus and resulted in almost complete degradation of epg1 mRNA. The need for a native promoter to selectively down-regulate specific gene loci was addressed by developing a carbon-catabolite regulated promoter (alcA) to drive the expression of the epg1-RNAi cassette. The expression of an alcA-driven epg1-RNAi cassette resulted in the down-regulation of epg expression under glucose starvation but normal levels of expression in high glucose. The expression could therefore be controlled by culture conditions. The second strategy explored the potential of using dsRNA viruses to vector disruptive RNAi cassettes. An isolate of O. novo-ulmi strain 93-1224 collected in the city of Winnipeg, was infected by two dsRNA mitoviruses which upon sequence characterization were named OnuMV1c and OnuMV7. To assess the transmissibility of this dsRNA virus the infected isolate 93-1224 was paired with three naive isolates of the related fungi O. ulmi and O. himal-ulmi. Through the use of nuclear and mitochondrial markers it was determined that the virus OnuMV1c may not rely on mitochondrial fusion for transmission but may have a cytoplasmic transmission route. This investigation of gene expression and manipulation has provided tools to help understand gene regulation in O. novo-ulmi. It has also added to our knowledge of mitoviruses, their transmission and potential use as a biological control. By enhancing our understanding of transmissible hypovirulence this work contributes to efforts to develop a new approach to target DED as well as a potential model for the control of other fungal diseases. / Graduate / 0307 / 0306 / 0369 / jscarneiro@hotmail.com Ophiostoma novo-ulm Dutch elm disease (DED) RNA interference Polygalacturonase Alcohol dehydrogenase promoter Gene expression Phytopathogen Cell wall degrading enzymes Pectin enzyme Mitoviruses Pathogenicity Virulence Double-stranded RNA (dsRNA) hyphal anastomosis group I and II introns Homing Endonuclease Genes (HEGs) Single Sequence Repeats (SSRs)
13	The Kamchay Hydropower Project -Hydropower development in Cambodia and eastern Asia Malmquist, Peter, Sigfridsson, Mats January 2003 (has links) The objective in this case study is to highlight typical problems of a planned hydropower dam project in Bokor National Park, Cambodia. The focal point in the line of questioning is the usage of public participation in larger exploration plans in a development country and to give a comprehensive survey of some of the participant involved. The study also discusses how the WCD report is used by non-govemental organisation to validate their claims. To be able to understand how the Cambodian society works, the study also includes a short briefing of recent Cambodian history. The study shows that the local people are more or less neglected in the preliminary investigations. Even if information meetings are held open, they are conducted in such a way that it is hard for common people to participate. The meetings are held in English and not in the domestic language Khmer. Critical groups are not formally invited but welcome to attend, most of their statements are withdrawn from the official protocols. The study also reveals that the leading entrepreneurs more or less neglects the environmental hazards in the construction by focus their information and calculations on the positive effects of the project. The study has been made as a field study, on location in Cambodia. hydropower eastern asia cambodia development mrc public participation kamchay khmer sida vattenkraft dammar folkligt deltagande deltagande miljöpåverkan mfs environment kambodia displacement ecosystems hydroelectric ngo mekong phnom penh kampot bokor wcd wcd-report cepa wildaid oxfam rural ded khmer rouge powergrid elproduktion dams turbines power production Engineering and Technology Teknik och teknologier

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