Made available in DSpace on 2017-07-21T20:56:31Z (GMT). No. of bitstreams: 1 Marta Lena Jahn.pdf: 1783494 bytes, checksum: c6497fd4a435d106076c444a8c6fc4cc (MD5) Previous issue date: 2013-04-11 / The objective of this study is to propose a different approach to teaching of matrices, seeking value the geometric aspect of them, and determinants. In fact, the matrices are important not only as a way to organize numerical data (tables), but also by the different properties of the operations that define the matrix algebra. However, the traditional way of teaching matrices and determinants in a secondary education, such as tables of numbers and a number associated with a matrix, respectively, the definitions of matrix operations and determinants, as well as their properties become quite unnatural. The Geometry not only turns these concepts more natural, but also develops our intuition about it. Furthermore, the geometrical aspects of the matrices and determinants are present in technological applications such as in Computer Graphics, for example. We begin this work by analyzing some textbooks adopted in secondary education, in order to see how the theory of matrices and determinants have been approached in classrooms, and to relate it to national education curriculum guidelines. Later, we explore the geometric content of matrices and determinants and propose a methodology for teaching, which brings out this aspect. This approach by middle of the Geometry is intended to stimulate the student to deduce properties and reflect on the results in operations with matrices, for example, addition, multiplication and also in the calculation of determinants. In proposing this methodology, we use the free software Geogebra, with the intent of providing a better view of the content for the student. / O objetivo deste estudo é propor uma abordagem diferente para o ensino das matrizes, buscando valorizar o aspecto geométrico delas, e dos determinantes. De fato, as matrizes são importantes não apenas como forma de organizar dados numéricos (tabelas), mas também pelas diversas propriedades das operações que definem a álgebra matricial. Todavia, na forma tradicional de ensinar matrizes e determinantes no ensino médio, como tabelas de números e um número associado a uma matriz, respectivamente, as definições das operações matriciais e determinantes, assim como as suas propriedades, tornam-se bastante artificiais. A Geometria não apenas ajuda a tornar estes conceitos mais naturais, como também desenvolve a nossa intuição sobre o assunto. Além disso, os aspectos geométricos das matrizes e determinantes estão presentes em aplicações tecnológicas tais como em Computação Gráfica, por exemplo. Começaremos este trabalho analisando alguns livros didáticos adotados no ensino médio, para verificar como a teoria de matrizes e determinantes vem sendo abordada em sala de aula; e relacionar essa teoria com os parâmetros curriculares nacionais do ensino médio. Posteriormente, exploraremos o conteúdo geométrico de matrizes e determinantes e proporemos uma metodologia para o seu ensino que valorize este aspecto. Esta abordagem por meio da geometria tem a finalidade de estimular o aluno a deduzir propriedades e refletir sobre os resultados obtidos nas operações realizadas com matrizes, por exemplo, a adição, a multiplicação e também, no cálculo de determinantes. Ao propor esta metodologia, faremos uso do software livre Geogebra, com o intento de propiciar uma melhor visualização do conteúdo pelo aluno.

Background: In this dissertation I examine the political economy of the post-socialist mortality crisis as experienced in deindustrialising towns in Hungary. I develop and apply a relational political economy of health framework, putting emphasis on the economic institutions of post-socialist dependent capitalism in Hungary, as embedded in the semi-periphery of the global economy, their gendered implications and their cultural construction. Methods: I follow a mixed-method strategy combining quantitative and qualitative analyses. I rely on a novel dataset comprising data on settlement, enterprise, and individual levels. 260 companies and 52 towns were analysed in two waves. I group towns into severely and moderately deindustrialised categories (1989-1995); as well as into dominant state, domestic private and foreign ownership dominated categories (1995-2004). Population surveys in these towns collected data on the vital status and other characteristics of survey respondents' relatives. I assess the relationship between deindustrialisation, dominant ownership and the mortality of individuals by random intercept multilevel discrete-time survival modelling. I also investigate the health implications of the lived experience of economic transformation in four towns with diverging privatisation and deindustrialisation histories through a qualitative thematic analysis of 82 in-depth semi-structured interviews. Findings: Severe deindustrialisation is associated with a significantly larger odds of mortality for men between 1989 and 1995 (OR=1.12; 95%CI=1.00-1.26; p=0.042). On the other hand, prolonged state ownership is related to a significantly lower odds of dying among women, compared to towns dominated by domestic private ownership (OR=0.74; 95%CI=0.62-0.90; p=0.002) or towns dominated by foreign investment (OR=0.79; 95%CI=0.65-0.96; p=0.019) between 1995 and 2004. The multi-sited semi-structured qualitative interviews revealed that companies are central institutions in the cognitive maps of workers and that the fates of these companies affected the health of workers in multiple ways, whereas state involvement was perceived as a cushioning mechanism. Interpretation: Severe deindustrialisation was a crucial factor behind the post-socialist mortality crisis for men, whilst prolonged state ownership was associated with the protection of life chances for women. The indirect economic benefits of foreign investment do not translate automatically into better health. Rapid economic transformations threaten health; they should be avoided where possible, but if this is not possible, strong safety nets should be in place.

Submitted by Fabricia Fialho Reginato (fabriciar) on 2015-07-06T22:24:23Z No. of bitstreams: 1 WagnerFraga.pdf: 725600 bytes, checksum: babf8f01e69058beb20dced3a858e983 (MD5) / Made available in DSpace on 2015-07-06T22:24:23Z (GMT). No. of bitstreams: 1 WagnerFraga.pdf: 725600 bytes, checksum: babf8f01e69058beb20dced3a858e983 (MD5) Previous issue date: 2014-11-20 / CNPQ – Conselho Nacional de Desenvolvimento Científico e Tecnológico / O objetivo do estudo é identificar os determinantes socioeconômicos relacionados às altas taxas de mortalidade por suicídio nos municípios do Brasil e, principalmente, no Rio Grande do Sul. Como metodologia, empregaram-se métodos para dados de contagem: Regressão de Poisson, Regressão Binomial Negativa e Regressão Quantílica para Dados de Contagem. Os resultados mostraram que entre os indicadores socioeconômicos desenvolvidos pela FIRJAN, apenas o IFDH Saúde demonstrou ser importante na determinação das mortes por suicídio nos municípios brasileiros. Contudo, os suicídios ocorridos nos municípios do Rio Grande do Sul, no período analisado, não demonstram ser influenciados pelo IFDH Saúde e Educação. Além de apresentar uma relação positiva com óbitos por violência auto infligida, a pobreza contribuiu de forma importante nos municípios com elevado número de mortes. A associação entre a taxa de desemprego e o número de mortes por suicídio mostra uma relação inversa, de maneira que se a taxa de desemprego dos municípios aumenta, a tendência é diminuir as mortes por suicídio. Mostrou-se também que um dos principais fatores que afetam o número de casos de suicídio tanto no Brasil quanto no Rio Grande do Sul é o nível de dependência agropecuária. Por fim, foi possível diagnosticar, também, que a característica cor/raça branca e indígena está entre os principais fatores determinantes das mortes por suicídio nos munícipios brasileiros. / This study aims to identify the socioeconomic factors related to high rates of suicide mortality in the cities of Brazil and, especially, in Rio Grande do Sul. The methodology employed involved methods for count data: Poisson regression, negative binomial regression, and quantile regression for count data. The results showed that among socioeconomic indicators developed by the FIRJAN, just the Health IFDH proved important in determining the suicide deaths in Brazilian cities. However, the suicides occurred in the cities of Rio Grande do Sul, in the period analyzed, not demonstrating being influenced by the Health and Education IFDH. In addition to presenting a positive relation with suicide deaths by self-inflicted violence, poverty is a major contributor in cities with high numbers of deaths. The association between the unemployment rate and the number of suicide deaths shows an inverse relationship; if the unemployment rate of cities increases, the tendency is to reduce deaths by suicide. It was also shown that one of the main factors affecting the number of suicide cases in Brazil and in Rio Grande do Sul is the level of agricultural dependence. Finally, it was possible to identify, also, that the characteristic color/ white and Indian race is among the main determinants of suicide deaths in Brazilian cities.

Submitted by Fabricia Fialho Reginato (fabriciar) on 2015-07-17T22:54:28Z No. of bitstreams: 1 JonasBorghetti.pdf: 898124 bytes, checksum: f0383febc6c23edc4d27b88be929aad2 (MD5) / Made available in DSpace on 2015-07-17T22:54:29Z (GMT). No. of bitstreams: 1 JonasBorghetti.pdf: 898124 bytes, checksum: f0383febc6c23edc4d27b88be929aad2 (MD5) Previous issue date: 2013 / Nenhuma / Os elevados níveis de evasão fiscal existentes nos países em desenvolvimento resultam em graves prejuízos sociais tais como a falta de justiça fiscal, crescimento da economia informal e perda de legitimidade do poder público. Assim, esta dissertação procurou determinar e analisar os fatores que afetam o nível de evasão fiscal nos países da América Latina. Para isso, utilizaram-se dados de pesquisa elaborada no âmbito do Centro Interamericano de Administrações Tributárias (CIAT) abrangendo o período de 2000-2010, além de indicadores sociais, econômicos e institucionais dos países medidos por uma série de organizações. A análise foi realizada por meio de um modelo de regressão múltipla utilizando dados em painel. Os resultados obtidos indicaram que quanto maior a média de idade da população do país, a qualidade da educação oferecida, o investimento público em pesquisa e desenvolvimento e a renda proveniente do trabalho assalariado urbano, menores os níveis de evasão fiscal. Por outro lado, o aumento na criminalidade e na desigualdade de renda gera crescimento no comportamento evasivo. No que diz respeito à maior complexidade da legislação, o efeito varia conforme a preponderância em uma relação existente entre o custo para o contribuinte, quando há crescimento no nível de evasão, e a utilidade da informação obtida como controle eficaz para o Fisco, que reduz a sonegação. / The high levels of tax evasion that exist in developing countries result in serious losses such as the absence of fair taxation, growth of the informal economy and loss of legitimacy of the government. Thus, this dissertation sought to determine and analyze the factors that affect the level of tax evasion in Latin America. For this, data from research carried out under the InterAmerican Centre of Tax Administrations (CIAT) covering the period 2000-2010 were used, as well as social, economic and institutional indicators of the countries measured by organizations. The analysis was conducted through a multiple regression model using panel data. The results indicated that the higher the medium age of the population, the quality of education provided, the public investment in research and development and income from urban employment, lower is the levels of tax evasion. On the other hand, the increases in crime and income inequality generate growth in evasive behavior. About the higher complexity of the legislation, the effect varies with the preponderance on the relationship between the cost to the taxpayer, when the level of tax evasion grow, and the usefulness of the information obtained as to effectively control the taxman, what reduces evasion.

Made available in DSpace on 2016-04-29T14:16:01Z (GMT). No. of bitstreams: 1 Fernanda da Silva Moura.pdf: 854487 bytes, checksum: 108cb480e532a1db11abd060da480ced (MD5) Previous issue date: 2011-06-21 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / This master's thesis aims to understand the process of health and disease as well as the social determinants that emerges during the hospitalization of children and adolescents admitted to the Pediatrics Ward of the University Hospital of the University of São Paulo. The study sought to clarify issues related to the subject's health as a result of his lifestyle or routine. The other purpose of the study was to identify the interdisciplinary team, whether to acknowledge the work of the Social Determinants of Health, or whether they remain within the concept of health with the absence of disease. The theoretical and historical materialism was used as a methodology to reflexive interview with the semi-structured. The field investigation led to the confirmation that practice is what distinguishes interdisciplinary team to work with is extremely complex demands. Family members interviewed demonstrated weakness in understanding the issues that go along with the sickness, but felt reassured by receiving attention from professionals. During this study the peculiarities of each group, professionals, and families were discussed. The reflections generated stronger bias in the work shared between the different areas, and multiply the total knowledge at the time of operation. In relation to the families there is a need for further understanding and familiarity of lifestyle. This is already somewhat happening, but the closer proximity to reality then the greater security the team will have in their behavior / A presente Dissertação de Mestrado visa apreender o processo de saúde e doença, bem como os determinantes sociais que emergem durante a hospitalização de crianças e adolescentes internados na Enfermaria Pediatria do Hospital Universitário da Universidade de São Paulo. O estudo buscou esclarecer questões relacionadas à saúde acolhendo o sujeito como protagonista de sua trajetória. A outra via de estudo foi identificar se a equipe interdisciplinar reconhece a atuação dos Determinantes Sociais da Saúde ou se permanecem no conceito de saúde como ausência de doença. Foi utilizado como aporte teórico o materialismo histórico e como metodologia a entrevista reflexiva com roteiro semi-estruturado. A investigação de campo permitiu a constatação de que a prática interdisciplinar é o que diferencia a equipe ao se atuar junto a demandas extremamente complexas. Os familiares entrevistados demonstraram fragilidade no entendimento das questões mais estruturais que os acompanham no processo de adoecimento, mas se sentem seguros ao receberem atenção dos profissionais. No desenvolvimento desse estudo foram discutidas as peculiaridades de cada grupo, profissionais e familiares. As reflexões geradas se fortalecem no viés do trabalho compartilhado entre as diferentes áreas, que somam e multiplicam saberes no momento de atuação. Em relação aos familiares existe a necessidade de maior aproximação da realidade vivenciada, isto já acontece, porém quanto mais próximo da realidade mais a equipe terá segurança em suas condutas

Le virus West Nile (VWN) est un virus neurotrope principalement transmis par piqûre de moustique et dont le réservoir est constitué par la faune aviaire sauvage. Les souches circulant en Europe appartiennent à 4 lignages génétiques différents à l’origine de nombreuses épidémies d’ampleur modérée à faible et limitées géographiquement, contrairement à ce qui a été observé en Amérique du Nord. En 1998 en Israël, une importante épidémie a a été associée pour la première fois à une forte mortalité de la faune aviaire sauvage. Le virus (souche IS-98-ST1, lignage 1a) a été isolé du cerveau d’une cigogne moribonde. L’objet de cette thèse a été de construire un clone infectieux de la souche IS-98-ST1 afin d’en explorer les propriétés de neuroinvasion et de pouvoir mettre en évidence les déterminants moléculaires de sa virulence.Le virus obtenu à partir de la construction clone infectieux s’est révélé posséder les mêmes propriétés biologiques que le virus parental, que ce soit in vitro sur cellules Vero ou in vivo sur souris sensibles ou résistantes ou encore sur l’embryon de poulet. L’embryon de poulet est présenté ici comme un nouveau modèle d’évaluation de la virulence du VWN. Un modèle cellulaire neuronale (lignée de neuroblastomes humains, SK-N-SH) est aussi évalué dans ce manuscrit. En conclusion, un nouvel outil de génétique inverse a été obtenu pour le VWN. Cet outil permettra de travailler sur l’impact de mutations ponctuelles, ou de modifications plus importantes touchant un ou plusieurs gènes viraux sur la virulence du VWN, spécifiquement dans le contexte européen. / West Nile virus (WNV) is a neurotropic virus mainly transmitted through mosquito bites. Wild birds represent the main reservoir hosts. Strains circulating in Europe belong to four lineages and have caused numerous but limited epidemics over the last few years. In 1998, an important outbreak associated to huge bird fatalities caused by a highly neuroinvasive strain (IS-98-ST1) took place in Israel. We aimed at producing a new infectious clone, based on the lineage 1a IS-98-ST1 WNV strain, for the characterization of its neuroinvasion properties as well as the molecular determinants of European WNV virulence. The growth kinetics of recombinant and parental WNV were similar in Vero cells. Moreover, the phenotypes of recombinant and parental WNV were indistinguishable in terms of viremia, viral load in the brain and mortality in susceptible and resistant mice. Finally, the pathobiology of the infectious clone was examined in embryonated chicken eggs, proposed as a new model for the evaluation of WNV virulence. The potential of human neuroblastoma cells (SK-N-SH) to discriminate between highly and mildly virulent WNV strains was assayed. In conclusion: a new molecular tool that is useful for the study of molecular determinants of WNV virulence has been generated. We take advantage of the high genetic stability of our one-piece infectious WNV cDNA clone to produce mutant viruses through the insertion of point mutations or the exchange of genetic fragments between WNV strains into the backbone of the IS-98-ST1 infectious clone.

Cette thèse s’intéresse à la contribution des expositions professionnelles —chimiques, biomécaniques, et psychosociaux—tout au long de la vie dans les inégalités sociales de santé liés au vieillissement. Les trois papiers de la thèse essaient de combler les lacunes dans les connaissances sur les effets à long terme des expositions professionnelles et la contribution des expositions individuelles et conjointes dans ces inégalités. Le premier papier cherche à savoir si le niveau d’éducation atteint dans l’enfance modifie l’effet de l’exposition professionnelle aux solvants au cours de la carrière sur la fonction cognitive après 55 ans.. Nous avons trouvé que l’effet de l’exposition aux solvants sur la fonction cognitive était différent selon le niveau d’éducation.. Le deuxième papier discute les effets combinés des expositions biomécaniques et psychosociaux au cours de la vie professionnelle sur la santé physique et fonctionnelle après la retraite. Nous avons trouvé qu’une forte exposition à la fois aux risques physiques et psychosociaux au cours de la vie active est associée à une capacité fonctionnelle bien plus faible après la retraite que l’exposition à un seul type de risques. Aussi, l’effet était différent chez les hommes et chez les femmes. Le troisième papier est un papier méthodologique qui discute des alternatives aux formes longues des échelles généralement utilisées pour évaluer les expositions biomécaniques au travail. Nous avons cherché à vérifier si la question « Trouvez-vous votre travail fatiguant physiquement?» pourrait être une mesure de substitution acceptable pour évaluer des expositions détaillées.. Nous avons comparé la mesure à un item à une mesure plus complète de 38 expositions spécifiques dans huit domaines. Nous avons trouvé que la mesure à un item était plus valide chez les participants fortement exposés à des contraintes biomécaniques de travail. En somme, cette thèse a montré que les expositions professionnelles peuvent à la fois exacerber les disparités existantes en matière de santé et les perpétuer au delà de la retraite. / This dissertation focuses on how occupational exposures throughout the lifecourse—chemical, physical, and psychosocial—contribute to social patterns in aging outcomes, addressing the gap in knowledge about lasting effects of occupational exposures and the contribution of individual and combined exposures to social patterns in aging. The first paper explores whether childhood educational attainment modifies effects of occupational solvent exposure during the career on cognitive function after age 55. We found differential effects of solvent exposure on cognition by educational attainment. The second paper looks at combined physical and psychosocial exposures during working life and effects on physical health and functioning after retirement. This paper finds that high exposure to both physical and psychosocial hazards at work is associated with worse functional capacity in retirement than exposure to either one separately; the pattern of this relationship differs for men and women. The third paper is a methodology paper exploring alternatives to extensive testing batteries often used to evaluate physical workplace exposures. We tested whether the question “Do you find your work physically strenuous?” was an acceptable proxy measure for more detailed exposure assessments by comparing the measure to a comprehensive self-report measure of 38 specific biomechanical strains across eight domains. We found that the measure was most valid in the populations that were most highly exposed to physical strains at work. In general, this dissertation found that occupational exposures can exacerbate existing disparities in health and perpetuate disparities into and beyond retirement.

The coastal landscape currently known as British Columbia, Canada represents a complex and rapidly evolving site of collaboration, negotiation, and conflict in environmental management, with important implications for Indigenous community well-being. I ground this work in the understanding that settler-colonialism and its remedies, resurgence and self-determination, are the fundamental determinants of Indigenous health and related inequities. Through a case study of eulachon (Thaleichthys pacificus) in Nuxalk territory, I take interest in systemic mechanisms of dispossession and resurgent practices of (re)connection and knowledge renewal as mediators of the relationship between environmental management and Indigenous health and well-being. This work is based in four years of observation, participation, and leadership in the Nuxalk Sputc (Eulachon) Project, a community-directed process that aimed to document and articulate Nuxalk knowledges about eulachon. Functionally extirpated from the region since 1999, these valued fish provide an example of contested management jurisdiction and resurgent Indigenous environmental practice. As a resurgent research and management process, the Sputc Project re-centered Nuxalk knowledges, voices, priorities, and leadership while advocating Indigenous leadership in environmental management. This case study was conducted within the context of the Sputc Project, aiming to share substantive and methodological learnings gleaned from the project, which served as an ideal focal point for the interrogation of relationships between Indigenous well-being, research methodologies, engagement and representation of Indigenous knowledges, and environmental management. Applying a critical, decolonising, community-engaged approach, this work comprises four papers, each drawing on a particular thread of the knowledge generated through this work. In Paper 1, I seek to establish the connection of eulachon and their management to Nuxalk health and well-being. Detailing three stages of this relationship (abundance, collapse, and renewal), I show how the effects of environmental management, and resulting dispossession or reconnection, are mediated by cultural knowledges, practices, responsibilities, and relationships. Turning to research methodology in Paper 2, I examine how Nuxalk people and knowledges guided the Sputc Project process, interrogating the role of critical, decolonising, and Indigenous theories in the elaboration of Indigenous research methods in environmental management and beyond. In Paper 3, I consider how the Sputc Project respectfully articulated and represented Nuxalk knowledges in order to retain relational accountability and strengthen Nuxalk management authority, while promoting values, practices, and relationships essential to Nuxalk well-being. In Paper 4, I demonstrate how the Sputc Project strengthened Nuxalk management authority from the ground up, detailing the practical management priorities that arose through the project process, including those related to interjurisdictional engagement of Indigenous leadership. I end with a reflection on this work’s implications for decolonising health equity and environmental impact assessment frameworks. Highlighting how Indigenous health and well-being is supported by ancestral knowledges and reconnecting relationships, including those involving people, places, and practices related to environmental management, I emphasize the importance of Indigenous leadership (vs. knowledge integration) in environmental management research and practice. A final section seeks to inform decolonising community-engaged research, sharing limitations and learnings related to appropriate engagement, articulation, and representation of Indigenous knowledges. / Graduate

La cardosine A est une protéase aspartique identifiée il y a plus de 20 ans dans les cellules du chardon Cynara cardunculus. Sa distribution dans tous les tissus de la plante et ses caractéristiques enzymatiques ont été caractérisées par approches biochimiques. La cardosine A a des fonctions essentielles dans la reproduction, la mobilisation de réserves protéiques, et le remaniement de membranes. Pour assumer ces différentes fonctions, la cardosine A doit pouvoir transiter et s’accumuler dans différents compartiments intracellulaires : vacuole de stockage, vacuoles lytiques, ou autres compartiments membranaires. Il n’y a cependant que très peu de données disponibles sur les mécanismes de biosynthèse, de tri, de transport et d’adressage aux différents compartiments cellulaires. De récents travaux suggèrent que l’expression en modèle hétérologue pourrait être utilisée pour une meilleure compréhension de la biologie intracellulaire de la cardosine A. Les résultats de cette étude montrent que l’expression transitoire de la cardosine A dans les feuilles de Nicotiana tabacum est un bon modèle expérimental pour explorer le transport de la cardosine A dans la cellule. En effet dans ce système les mécanismes de maturation et de transport de la protéine à la vacuole sont conservés. De plus, une lignée stable d’Arabidopsis thaliana exprimant la cardosine A sous promoteur inductible s’est également avérée un bon modèle d’étude du transport intracellulaire de la cardosine A. L’utilisation de ces systèmes hétérologues a permis de combiner l’expression de formes mutées de la cardosine A (dans lesquelles des séquences spécifiques ou des acides aminés avaient été tronqués ou modifiés) avec des approches de biochimie et d’imagerie cellulaire pour identifier des signatures moléculaires responsables de l’adressage vacuolaire de la protéine. Nos résultats montrent que la cardosine A a deux déterminants vacuolaires dans sa séquence protéique : le domaine “PSI”, qui définit un déterminant d’adressage vacuolaire original et propre à certaines protéases aspartiques, et un peptide C-terminal appartenant à la classe bien définie des ctVSD. De plus, les résultats montrent que la présence de ces deux déterminants illustre la capacité d’emprunter deux routes distinctes pour atteindre la vacuole : le domaine PSI peut permettre d’attendre la vacuole sans passer par le Golgi, tandis que le domaine C-ter négocie un transport classique Reticulum, Golgi, Prévacuole, Vacuole. Cette capacité à choisir deux routes différentes n’est pas observée pour la cardosine B, autre protéase aspartique du chardon présentant une haute homologie de séquence avec la cardosine A. Pour expliquer cette capacité de la cardosine A à emprunter deux routes vacuolaires différentes, l’hypothèse d’un rôle possible de la glycosylation dans le tri des protéines entre les deux routes vacuolaires est alors étudiée. L’expression de la cardosine A dans les graines en germination d’Arabidopsis thaliana révèle que la protéine peut s’accumuler d’une manière différentielle dans les graines en absence de germination ou pendant la germination, tout au long du système endomembranaire jusqu’à la vacuole de réserve ou dans les vacuoles lytiques en formation. Les expériences de blocage de transport du Reticulum au Golgi n’ont pas permis de conclure d’une manière certaine si les accumulations vacuolaires dérivaient d’un transport pouvant court-circuiter le Golgi comme dans les feuilles de Nicotiana. Au total, la cardosine A constitue une protéine modèle pour étudier les transports vacuolaires chez Nicotiana tabacum and Arabidopsis thaliana, deux systèmes hétérologues qui permettent de développer des méthodes complémentaires pour une exploration fonctionnelle des mécanismes impliqués. Cette étude permet de contribuer à une meilleure connaissance de la biologie des cardosines en particulier, et des protéases aspartiques en général. / The aspartic proteinase cardosin A is a vacuolar enzyme found to accumulate in protein storage vacuoles and lytic vacuoles in the flowers and in protein bodies in seeds of the native plant cardoon. Cardosin A has been first isolated almost two decades ago and has been extensively characterized since, both in terms of distribution within the tissues and of enzyme biochemistry. In the native system, several roles have been addressed to cardosin A in reproduction, mobilization of reserves and membrane remodeling. To participate in such diverse events, cardosin A must accumulate and travel to different compartments inside the cell: protein storage vacuoles, lytic vacuoles, cytoplasmic membrane (and eventually outside the cell). However, not much information is available regarding cardosin A biogenesis, sorting or trafficking to the different compartments. Recent studies have approached the expression of cardosin A in Arabidopsis thaliana and Nicotiana tabacum. These preliminary observations were the starting point of a detailed study of cardosin A expression, localisation, sorting and trafficking routes, resourcing to several and very different methods. It has been showed that transient expression of cardosin A in Nicotiana tabacum leaf is a good system to explore cardosin A trafficking inside the cell, as the protein is processed in a similar manner as the control and accumulates in the vacuole. Furthermore, an Arabidopsis thaliana line expressing cardosin A under an inducible promoter was explored to understand cardosin A dynamics in terms of vacuolar accumulation during seed germination events. Similarly to the Nicotiana tabacum one, this system was also validated for cardosin A expression and it allowed to conclude that the protein’s expression did not retrieved any phenotype to the cells or individuals. However, experiments conducted in BY-2 cells revealed to be inconclusive since cardosin A expression in this system is not predictable. The data obtained along this work using several cardosin A mutated forms, lacking specific domains or point-mutated, allowed to determine that cardosin A has two Vacuolar Sorting Determinants in its protein sequence: the PSI, an unconventional sorting determinant, and the C-terminal peptide, a C-terminus sorting determinant by definition. Furthermore, it was also demonstrated that each domain represents a different route to the vacuole: the PSI bypasses the Golgi Apparatus and the C-terminal peptide follows a classic Endoplasmic Reticulum-Golgi Apparatus-Prevacuole route to the vacuole. This difference in the trafficking routes is not observed for cardosin B sorting determinants as both the PSI and C-terminal peptide from cardosin B needs to pass the Golgi Apparatus to reach the vacuole. A putative role for glycosylation in the trafficking routes is further discussed as cardosin A PSI, contrary to cardosin B, is not glycosylated. The production of mutants affecting cardosin A glycosylation sites supported this idea. Moreover, cardosin A expression in germinating Arabidopsis thaliana seeds revealed a differential accumulation in non-germinated and germinated seedlings. Cardosin A was detected along the secretory pathway to the Protein Storage Vacuole in association with the Endoplasmic Reticulum, Golgi Apparatus, Prevacuole and newly formed Lytic Vacuoles. The drug Brefeldin A caused the protein to be retained in the Golgi Apparatus, despite some amount being still detected in the vacuole, not being clear if the Golgi Apparatus bypass observed in Nicotiana tabacum leaves occurs in this system. As a whole, cardosin A confirmed to be a good model to study vacuolar sorting in these two systems that complement each other in terms of approaches available. This study provided good results in order to understand in more detail cardosin A biology in particular and vacuolar trafficking of plant Aspartic Proteinases as a group.

Introdução: O Brasil é um país bastante extenso territorialmente, sendo necessário o mapeamento da situação do aleitamento materno em todo o país. Objetivo: Descrever a situação do aleitamento materno (AM), no Brasil e estudar o efeito de determinantes sociais, econômicos e demográficos sobre o AM de crianças menores de um ano de idade. Método: Constituiu-se amostra de 2958 crianças, divididas em dois grupos de 0 a seis meses, com 1477 crianças e de 7 a 12 meses com 1481 crianças, representativas da população nacional. Os dados fazem parte da Pesquisa de Orçamentos Familiares - POF, realizada em 2002-2003. Para o estudo do AM no Brasil, utilizou-se um modelo multinível com dois níveis de hierarquia. Para a análise do AM nas regiões utilizou-se modelo GLM com link=log, que permite o uso da razão de prevalências e intervalos com 90% de confiança. Resultados: No grupo 0 a 6 meses, a freqüência do AM no Brasil foi de 58% [IC90% 55 - 60]. Segundo as regiões, as freqüências foram de 63%, 59%, 51%, 61% e 56%, respectivamente para Norte, Nordeste, Sudeste, Sul e Centro-Oeste. Nas áreas rurais e urbanas a freqüência foi de 60% e 58%, respectivamente. No grupo de 7 a 12 meses a freqüência no Brasil foi de 35% [IC90% 33 - 38]. Nas regiões, as freqüências foram: 44%, 34%, 37%, 34%, 28%, respectivamente para Norte, Nordeste, Sudeste, Sul e Centro-Oeste. Nas áreas rurais e urbanas a freqüência foi de 39% e 34%, respectivamente. Consideraram-se como fatores desfavoráveis ao aleitamento materno, no conjunto da amostra: mães com idades superiores a trinta anos, quatro ou mais moradores no domicílio e uso de creche. Os fatores favoráveis foram: ter duas ou mais crianças menores de cinco anos no domicílio, mães de cor negra ou parda e maior renda. Conclusão: Nos dois grupos de idade, a freqüência do AM foi maior na região Norte e áreas rurais do país. Os fatores que determinam o AM diferem quanto a faixa etária, sendo importante focar ações de promoção da prática da amamentação também em crianças de seis a vinte e quatro meses. / Introduction: Brazil is a very large country and it is necessaries to mapping the breastfeeding situation around the country. Objective: To describe the situation of breastfeeding (BF), in Brazil, to study the effect of social, economic and demographic determinants on the BF of children under one year of age. Method: This sample is constituted of 2958 children, divided into two age groups: from 0 to 6 months composed by 1477 children and from 7 to 12 months composed by 1481 children, representative of the national population. The data are part of the Search for Family Budgets - POF, held in 2002-2003. For the study of BF in Brazil, it was used a multilevel model, with two levels of hierarchy. For the analysis of BF in the regions it was used the GLM model with link = log, which allows the use of prevalences ratio and intervals with 90% confidence. Results: In the age group 0 to 6 months, the frequency of BF in Brazil was 58% [IC90% from 55 to 60]. According to the regions, the frequencies were 63%, 59%, 51%, 61% and 56% respectively for North, Northeast, Southeast, South and Central West. In urban and rural areas the frequencies were 60% and 58% respectively. In the age group 7 to 12 months the frequency in Brazil was 35% [IC90% from 33 to 38]. In the regions, the frequencies were: 44%, 34%, 37%, 34% and 28% respectively for North, Northeast, Southeast, South and Central West. In urban and rural areas the frequencies were 39% and 34% respectively. The unfavorable factors for breast feeding throughout the sample were: mothers over the age of thirty years, four or more people living at home and use of children daycare center. The favorable factors were: having two or more children under five years at home, black or brown mothers and high income. Conclusion: In both age groups, the frequency of BF was higher in the northern and rural areas of the country. The factors that determine the AM differ by age, and it is important to focus actions to promote the practice of breastfeeding also in children from six to twenty-four months.