Haematopoietic stem and progenitor cells (HSPCs) are crucial for lifelong blood cell production. We analysed the cell cycle and cell production rate in HSPCs in murine haematopoiesis. The labelling of DNA-synthesizing cells by two thymidine analogues, optimized for in-vivo use, enabled the determination of the cell cycle flow rate into the G2-phase, the duration of the S-phase and the average cell cycle time in Sca-1+ and Sca-1- HSPCs. The determination of cells with 2n DNA content and labelled during the preceding S-phase was used to establish the cell flow rates in the G1-phase. Our measurements revealed a significant difference in how Sca-1+ and Sca-1- HSPCs self-renew and differentiate. The division of Sca-1+ progenitors led to the loss of the Sca-1 marker in about half of newly produced cells, corresponding to asymmetric cell division. In contrast both Sca-1- progenitors, arising from mitotic cell division, entered a new round of the cell cycle. This corresponds to symmetric self-renewing cell division. The novel data also enabled us to estimate the cell production rates in the Sca-1+ and in three subtypes of Sca-1- HSPCs. We focused on adult murine erythroid differentiation in the next part of our study. We introduced an original flow cytometry approach for identifying and studying erythroid...

This thesis explores the regional differentiation of electoral behaviour and other topics in electoral geography in the Czech Republic. Electoral geography, which studies the geographic aspects of the organisation, process, and results of elections, for a long time developed mainly in Western societies given the absence of free elections in other countries. It began to develop in the Czech Republic in the 1990s and its goal was to explore the process of the transformation of society and the stabilisation of democracy, to compare electoral patterns observed in conditions of stable liberal democracies with the patterns found in transforming states, and to adapt them to the specific conditions of transition countries. Electoral geography focuses mainly on the spatial variations of electoral behaviour. It seeks to determine whether different patterns of electoral behaviour are based on the social structure of the population in the region, whether they are influenced by contextual factors, or whether it is a combination of both. Another important subject studied by electoral geography is the spatial patterns of representation, which however are usually studied in majoritarian electoral systems. The thesis is composed of two basic sections. The first section offers a broader theoretical and...

Cultivation of cells in bioreactors with mechanical load simulates the physiological conditions to which cells in the body are exposed. This technology has been used to induce the differentiation of stem cells from adipose tissue towards the phenotype of vascular smooth muscle cells, which can further serve to form vascular replacements. At present, there is no established strategy for cultivating stem cells while being exposed to mechanical stress. The main aim of this work was therefore to optimize the cultivation strategy and determine the ideal load parameters. Differentiation was analyzed by immunofluorescence of specific smooth muscle cell markers, α-actin and h1-calponin, which were quantified by Western blot. Extracellular matrix production was also detected by immunofluorescence staining. The outcome of this work is the establishment of ideal conditions of cell culture in a bioreactor with mechanical load, during which they differentiate into smooth muscle cells. Three types of scaffolds were used for cultivation; plasma treated glass, fibrin-coated glass and decelularized pericardium. Preliminary results show that smooth muscle differentiation was succesfully induced in human and porcine adipose tissue stem cells. Cells were analyzed after 3 and 7 days of culture. Developing a stem cell...

Hematopoiesis is the process of proliferation, differentiation and self-renewal of hematopoietic stem cells. Regulation of hematopoiesis is a complex process, which takes place on many different levels and is directed by many signals. RNF207 is one of the perspective genes chosen based on a screen in chicken model, where obtained data show its role in hematopoiesis. The aim of this work was to confirm the role of rnf207b as a new regulator of hematopoiesis in Danio rerio and to find out on which level of hematopoiesis is active. Danio rerio is an excellent model to study the function of genes in vivo, thanks to the easy manipulation of genetic expression and wide range of phenotypes during the development. To study the effect of rnf207b in hematopoiesis of Danio rerio we performed the knock-down of this gene by microinjection of morpholino oligonucleotides into one cell stage embryos. In these injected fish, we saw the effect in both thrombocyte and erythroid lineage, suggesting that rnf207b could be a regulator at the hierarchical level of progenitors or even more upstream. The results of developmental and tissue specific expression analysis then show that expression of rnf207b begins as early as 18 hpf, at the time of primitive hematopoiesis. Although rnf207b is expressed in the kidney (an...

- v anglickém jazyce This diploma thesis called 'Differentiation in English language teaching at the elementary school' deals with employing differentiated instruction in a fifth-grade class at an elementary school in order to make learning more effective by balancing the stronger and the weaker students' needs. The theoretical part focuses on topics of learning and effective learning with an emphasis on English language teaching, mixed ability classes, learner differences and differentiated instruction. The final chapter of this part proposes a set of example activities and ideas that might be used in mixed-ability classes. The practical part uses action research to determine whether differentiate instruction was employed successfully. The outcomes of the research are discussed at the end of this part. The assumption is that after the research is finished, the situation in the class will improve since all students will feel adequately engaged in the lessons.

Myelodysplastic syndromes (MDS) represent a group of clonal stem cell disorders characterized by ineffective hematopoiesis, peripheral cytopenia, morphological dysplasia and the risk of transformation to acute myeloid leukemia (AML). MDS belongs to one of the most common hematological diseases in patients over 60 years old. MDS incidence is still increasing. Appropriate therapy of MDS remains challenging. There is no curative approach besides peripheral stem cells transplantation, which is regretfully appropriate only for a small group of patients due to a higher median age of the MDS population. This is why the search for therapeutic alternatives remains paramount. MDS treatment was rather frustrating until the recent introduction of two new therapeutic approaches: immunomodulation therapy with lenalidomide and epigenetic or demethylating therapy with 5-azacytidine. Both new drugs have significantly higher effect than standard therapy. However, the precise mechanism of this effect remains unknown. As a result, we decided to initiate several research projects while introducing this promising treatment to our patients. Our aim is to investigate the mechanism of both agents in relation to disease pathogenesis by examining changes of certain occurrences and factors prior to and during the course of...

Nuclear receptors constitute a large family of transcription factors that are powerful regulators of animal tissue metabolism, homeostasis, tissue maintenance and development. They are particularly attractive for their ability to respond to the binding of hormones, metabolites, xenobiotics and artificially prepared molecules and transmit the interaction with these small lipophylic molecules to specific regulatory potential. In search for nuclear receptors that are likely to be critical for neural tissues in invertebrates and conserved during the evolution of animals, we have identified a close homologue of vertebrate TLX in a planarian Schmidtea mediterranea. Planaria represent very promising biological model systems for studies on tissue maintenance and regeneration. Planaria are able to resorb their tissues and use them as sources of energy during fasting and they re-build their bodies from neoblasts when food is plentiful. Our search in Schmidtea mediterranea's publicly accessible genome sequencing data indicated that planarian genome contains at least one gene with a high degree of similarity to vertebrate TLX. We cloned full length CDS (coding DNA sequence of cDNA) and characterized the gene functionally. This showed that the planarian and vertebrate NR2E1 are highly similar...

The thesis deals with the faculties of education from the perspective of their role in the system of tertiary education in the Czech Republic. It works on the theoretical assumptions of the role of non-profit organisations, which emphasize the role of the mission and it's fundamental value for the existence of the entire institution. The author of the thesis works with the assumption in terms of values, which expresses the need of more transparent differentiation of the segment of the faculties of Education in the Czech higher education system. The fundament of the methodology is the studies and analysis of the documents together with expert questionnaire survey. Based on the methodology, the definition and formulation of the identity of the faculties of education is investigated on the level of legal documents, higher education legislation, institutions (statute of the faculty) and personal attitudes of senior academic staff (expert survey).

Regional differentiation is a complex phenomenon concerning all countries of the world, which is analyzed by countless studies, but without a generally accepted theoretical anchorage. The Thesis deals with the transformation of Turkish regional image at multiple levels from 1975 to 2018. This transformation is analyzed by a set of selected demographic and socioeconomic indicators. The main objective is to document and evaluate Turkish regional demographic and socioeconomic differentiation, which leads to the country's polarization. The Thesis is based on statistical data from the Turkish Statistical Office. The geographical concentration of demographic and socioeconomic indicators was illustrated by tabular and map outputs. Sigma convergence evaluating the evolution of differentiation of individual indicators Gini coefficients are shown by graphs. The multiple regression analysis method examined the impact of geographical aspects on selected demographic and socioeconomic indicators. Significant part of the Thesis also deals with Turkish political- geographical context and the theoretical approaches on which it is based.

This thesis deals with the development of fertility in countries and regions of the European Union in the context of convergence and divergence trends. The goal is to analyze and evaluate the basic trends in international and regional differentiation of fertility by studying the literature and by my own analysis trying to explain the observed spatial differences and to identify problems in methodology and data collection. The European Union is for the purpose of analysis divided into western and eastern part in order to respond better to the hypothesis defined in the introductory chapter. First, the analysis of the regional differentiation of fertility rate is made. Variability in fertility at the regional level is consequently successfully explained by international differences which allow you to move the analysis at the state level. International differentiations of fertility rate are then explained by the different position of states in the process of second demographic transition. Despite the expectations, a clear trend towards convergence in fertility levels was not observed in the western part of the European Union and its spatial patterns remained stable. The eastern part experienced a significant decrease in the intensity of fertility which caused its convergence and overshadowed the ongoing changes...