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Volatile Fatty Acids (VFAs) are a by-product when producing methane through anaerobic digestion (AD). Due to their many uses as building block chemicals, it is of interest to look into ways to optimize anaerobic digestion toward VFA production instead of methane generation. This report will focus on different parameters to produce VFA from food waste (FW), primary sludge (PS), and digested sludge (DS) in different ratios. In this project, three different experimental sets were run over a period of 25 days. The three different sets were 100 % FW, 100 % PS, and 50/50 % FW and PS mixture. Reactors were adjusted to an initial pH-value of 10 and then sparged with nitrogen to create an anaerobic environment. Measurements of the pH were done by sampling at each retention time. Gas chromatography (GC) was used at the end of the project to determine the concentrations of the VFA in the samples. The results showed that, with pH 10, a retention time of 15 days and using only FW, was optimal for VFA production as it gave the highest total concentration of 14.03 g VFA/L. Acetic acid was found in the highest concentration in all ratios. A mixture of FW and PS had an optimal retention time of 12 days, but did not generate as high concentrations of VFAs as only using FW, with a maximum concentration of 9.34 g VFA/L. Using only PS generated even lower concentrations, with a maximum of 5.33 g VFA/L, but did not start decreasing during the experimental run, and no clear conclusion can, therefore, be drawn.

The focus of the current study was on the recovery of carbon from municipal bio-waste streams. Firstly, the relationship between methanogenic pathways and the properties of anaerobic granules was studied using two pilot-scale upflow anaerobic sludge blanket (UASB) reactors with different granule size distribution. UASB1 granules were larger (3-4 mm) with multi-layered internal structure and the archaeal community was dominated by acetoclastic methanogens, while the UASB2 granules were smaller (1-2 mm) without a layered internal structure and the archaeal community was predominated by hydrogenotrophic methanogens. The acetoclastic methanogenic activities of UASB1 (250-437 mL CH4/g VS·d) than UASB2 (150-260 mL CH4/g VS·d), confirming that acetoclastic methanogenesis was more dominant in UASB1. Temperature increase from 20oC to 28oC did not change the microbial community but resulted in increased and stable biogas production for both reactors. There was an increase in chemical oxygen demand (COD) with hydraulic retention time due to increased contact time. The second part of the study focused on anaerobic co-digestion to produce volatile fatty acid (VFA) due to its higher value. The impact of substrate ratio of primary sludge (PS) and external organic waste (OW) and the robustness of the VFA system in the long term were assessed. Lab-scale batch study with different proportions of PS and OW; 0%, 25%, 50%, 75%, 100% of OW in terms of COD and scaled up in a semi-continuous pilot reactor with substrate ratio of 50% OW based on the results of the lab-scale study were performed. There was an increase in VFA production with an increase in OW proportion due to the availability of biodegradable organics. Acetic acid was the most dominant VFA in the batch reactors while in the semi-continuous experiment, caproic acid was dominated (50%). As carbon sources, the VFA-rich liquid attained the highest specific denitrification rate when compared with acetate and methanol. / I kommunala avfallsströmmar finns det en stor potential för resursåtervinning på grund av det höga organiska innehållet vilket kan användas för biobaserade produkter. På grundval av detta så fokuserar denna studie på resursåtervinningen av kol från kommunala avfallsströmmar genom att undersöka nuvarande och nytillkomna tekniker för att få vattenreningssystem att också bli resursåtervinningssystem. Första delen av studien undersöker relationen mellan metanproduktion och karaktäriseringen av anaerobiska granuler med ändamålet att förbättra energiproduktionen genom direkt behandling av kommunalt avloppsvatten. För detta ändamål så användes två UASB (Upflow Anaerobic Sludge Blanket) pilotreaktorer. Reaktorerna kördes med olika stora granuler, olika temperaturinställningar och olika hydrauliska uppehållstider (HRTs). Storlek, mikrobiell struktur, intern mikrostruktur och specifik metanogenaktivitet av de anaerobiska granulerna analyserades. Granulerna i UASB1 var 3-4 mm stora med interna multilager av mikrostrukturer som bestod av acetoklastika-metanogen arkéer. Granulerna i UASB2 var 1- 2 mm stora utan interna multilager och bestod av hydrogenotrofa metanogener. Aktiviteten i granulerna i UASB1 var 250-437 mL CH4 /g VS·d och i UASB2 var det 150-260 mL CH4 /g VS·d, vilket bekräftar att de acetoklastisk-metanogesa var mer effektiva i UASB1 än de hydrogenotrofa i UASB2. Att öka temperaturen från 20 o C till 28 o C under försöket medförde ingen förändring av den mikrobiologiska strukturen, men gav en ökning i biogasproduktion i UASB1 och högre och stabilare biogasproduktionshastighet i UASB2. Ökningen av biogasproduktion berodde på en reduktion i metans löslighet i utflödet och stabilare metanogenes. Ökning i HRT resulterade i större reduktion av organiskt material mätt som kemisk syreförbrukning (COD) och större biogasproduktion på grund av längre kontakttid mellan substrat och mikroorganismer. Den andra delen av studien fokuserar på att utveckla ett tillvägagångssätt att få en anaerob rötning att producera flyktiga fettsyror (VFA) istället för biogas. Studien fokuserar på effekten som kvoten mellan substraten primärslam och externt matavfall (OW) har och hur robust VFA-systemet skulle vara i stor skala på lång sikt. Olika kvoter av primärslam och matavfall testades i labbskala i batchstudier med 0 %, 25 %, 50 %, 75 % och 100 % COD OW. Baserat på resultaten från labbskala så kördes 50% COD OW i ett semi-kontinuerligt pilotförsök. Batch-testerna i labbskala visade att högre % COD OW, gav högre VFA produktion på grund av högre koncentration av organiskt material. Ättiksyra var den mest förekommande VFAn i batch-testerna medan kapronsyra var högst förekommande (50%) i det semi-kontinuerliga försöket. Denitrifikationsförsök visade att VFA-rik vätska från pilotskalareaktorerna gav den högsta specifika denitrifikationshastigheten i jämförelse med acetat och metanol. Resultaten visar att värdefulla kolkällor kan återvinnas från kommunalt avfall genom anaerob behandling av kommunalt avloppsvatten och samfermentering av primärslam och matavfall. / <p>QC 2020-06-15</p>

The aim of the project was to investigate the possibility of producing and utilizing bioenergy from available local resources in the village Linga Linga, Mozambique. Suitable conversion techniques for producing and utilizing bioenergy were identified through a literature study. The investigated techniques were the concept of gasifier cookstoves, the method of producing charcoal from biomass and anaerobic digesters. Through observations and interviews in the village, available local resources suited for the conversion techniques were identified. In the field study, it was found that there is a surplus of solid biomass which led to the conclusion that a gasifier cookstove is suitable to implement. In order to analyze if a gasifier cookstove is suitable for the households, interviews were carried out in ten households in the village. Aprototype of a gasifier cookstove was built with local resources to determine if the technique can be applied. The prototype was tested and evaluated in order to analyze if it will contributeto a more efficient use of resources. One of the conclusions of the study was that a gasifier cookstove can be valuable for the households in several ways, but that cultural differences can make it hard to implement.

Thesis (Ph. D. (Animal Production)) -- University of Limpopo, 2020 / A study was conducted to determine the effect of dietary crude (CF) levels of (3, 4, 5 and 7 %) on feed intake, digestibility, growth rate, feed conversion ratio (FCR) and live weight of male Ross 308 broiler and indigenous Venda chickens aged 1 to 42 days. The study, also, determined the effect of dietary CF level on the gastrointestinal morphology and digesta pH of gut organs of male Ross 308 broiler and indigenous Venda chickens aged 42 days. Dietary CF levels affected (P<0.05) feed intake, growth rate and live weight of male Ross 308 broiler and Venda chickens aged 1 to 21 days. A dietary CF level of 3.9 % optimized feed intake, while 4.5 % dietary CF optimized growth rate and live weight in male Ross 308 broiler chickens. However, dietary CF levels of 4.4, 4.8, 5.9 and 4.7 % optimized feed intake, growth rate, FCR and live weight, respectively, of male Venda chickens aged 1 to 21 days. Therefore, dietary CF level for optimal productivity depended on the breed of the chicken and production parameter of interest. Higher dietary CF levels decreased (P<0.05) crude protein (CP), neutral detergent fibre (NDF) and acid detergent fibre (ADF) digestibility values in male Ross 308 broiler chickens. Dietary CF levels of 3.8, 3.7 and 4.1 % optimized dry matter (DM) digestibility, metabolizable energy (ME) intake and nitrogen retention, respectively, in male Ross 308 broiler chickens aged 14 to 21 days. Increased dietary CF level, also, decreased (P<0.05) NDF and ADF digestibility values in male Venda chickens aged 14 to 21 days. Dry matter and CP digestibility values, ME intake and nitrogen retention of Venda chickens were optimized at dietary CF levels of 3.5, 3.7, 3.3 and 4.1 %, respectively. Feed intake of male Ross 308 broiler chickens aged 22 to 42 days were affected (P<0.05) by dietary CF level; and it was optimized at a dietary CF level of 6.4 %. Increased dietary CF level resulted in poorer growth rate, FCR and live weight of male Ross 308 broiler chickens. However, dietary CF levels of 4.5, 5.8, 6.4 and 5.7 %, optimized feed intake, growth rate, FCR and live weight, respectively, of male Venda chickens aged 22 to 42 days. Dietary CF levels of 3.4, 4.4, 3.7 and 4.4 %, optimized DM, CP and NDF digestibility values, and nitrogen retention, respectively, in male Ross 308 broiler chickens. However, dietary CF levels of 5.1, 5.3, 4.9, 10.1 and 5.1 % optimized DM, CP, NDF and ADF digestibility values, and nitrogen retention, respectively, of male Venda chickens. Therefore, dietary CF level for v optimal response in the chickens depended on breed, age and production variable of interest. The GIT weight of male Ross 308 broiler chickens was optimized at a dietary CF level of 4.1 %. In increased dietary CF level in male Ross 308 broiler chickens increased gizzard weights and decreased small intestine weights (P<0.05). Dietary CF levels of 6.3, 5.9 and 8.0 % optimized GIT, gizzard and caecum weights, respectively, in male Venda chickens. The small intestine weight of male Venda chickens was not affected (P<0.05) by dietary CF level. Caecum weight of male Venda chickens increased (P<0.05) with higher dietary CF level. However, caecum weights of male Ross 308 broiler chickens were not affected (P>0.05) by dietary CF level. The GIT and small intestine lengths were affected (P<0.05) by dietary CF level in male Ross 308 broiler chickens. Dietary CF levels of 5.6 and 5.5 % optimized GIT and small intestine lengths, respectively. However, in male Venda chickens, GIT and small intestine lengths were not affected (P>0.05) by dietary CF level. Digesta pH of the proventriculus and gizzard were affected (P<0.05) by dietary CF level in male Ross 308 broiler and Venda chickens. Different dietary CF levels of 5.5 and 7.4 % optimized the proventriculus and gizzard digesta pH in male Ross 308 broiler chickens, respectively. However, dietary CF levels of 4.2 and 4.3 % optimized the proventriculus and gizzard digesta pH values, respectively, in male Venda chickens. The two breeds of chickens had similar digesta pH values of the crop, proventriculus, gizzard and large intestines. However, male Venda chickens had higher (P<0.05) small intestine digesta pH values than male Ross 308 broiler chickens aged 42 days. Caecum digesta pH values of Ross 308 broiler chickens were higher (P<0.05) than those of Venda chickens aged 42 days. The second study was conducted to determine the effect of sodium bicarbonate supplementation level in the drinking water on feed intake, digestibility, FCR, growth rate, gut organ weight, length and digesta pH of male Ross 308 broiler and Venda chickens aged 22 to 42 days. The study, also, determined the effect of sodium bicarbonate supplementation level in drinking water on types of bacterial species in crop and gizzard digesta, as well as its effect on meat quality of male Ross 308 broiler and indigenous Venda chickens. Increased sodium bicarbonate vi supplementation level increased (P<0.05) water pH. Supplementation levels of 8.9, 2.04, 2.97 and 2.97 g of sodium bicarbonate per litre of drinking water optimized water intake, feed intake, growth rate and live weight of male Ross 308 broiler chickens, respectively. In male Venda chickens, there was a strong and positive relationship between sodium bicarbonate supplementation level and water intake of Venda chickens. A single supplementation level of 3.8 g of sodium bicarbonate per litre of drinking water optimized growth and live weight of male Venda chickens. There was a negative relationship between sodium bicarbonate supplementation level in the drinking water and NDF digestibility of male Ross 308 broiler chickens. Supplementation levels of 2.63, 6.67 and 7.0 g of sodium bicarbonate per litre of drinking water optimized DM and CP digestibility values, and nitrogen retention, respectively, in male Ross 308 broiler chickens. However, supplementation levels of 3.2 and 4.52 g of sodium bicarbonate per litre of drinking water optimized DM and NDF digestibility values, respectively, in male Venda chickens. There were negative relationships between sodium bicarbonate supplementation level in the drinking water and CP digestibility and nitrogen retention of male Venda chickens. Supplementation levels of 5.7, 2.2, 3.8, 7.6 and 7.2 g of sodium bicarbonate per litre of drinking water optimized proventriculus, gizzard and small intestinal weights, and GIT and small intestines lengths, respectively, in male Ross 308 broiler chickens. However, a sodium bicarbonate supplementation level of 2.8 % optimized proventriculus weights of male Venda chickens; the other digestive organ weights and lengths of Venda chickens were not affected (P>0.05) by sodium bicarbonate supplementation level. Sodium bicarbonate supplementation in the drinking water affected bacterial species found in the crops and gizzards of the chickens. There were different bacterial species found in the crop and gizzard digesta of male Ross 308 broiler and Venda chickens. Meat colour (L*, a* and b*) of the breasts and thighs of both breeds of chickens were not affected (P>0.05) by sodium bicarbonate supplementation. However, the redness (a*) values of the breast meat of male indigenous Venda chickens were higher (P<0.05) than those of breast meat from male Ross 308 broiler chickens. Sodium bicarbonate supplementation level did not affect (P>0.05) breast meat tenderness, juiciness, flavour and overall acceptability values and meat pH of Ross 308 broiler chickens. Supplementation levels of 3.6, 3.2 and 4.7 g of vii sodium bicarbonate per litre of drinking water optimized meat juiciness, flavour and shear force of male Venda chickens, respectively. It was concluded that sodium bicarbonate supplementation in the drinking water affected growth, NDF digestibility and bacterial species composition of male Ross and Venda chickens. Sodium bicarbonate supplementation level for optimal response in chickens depended on the breed and production variables of interest. / VLIROUS

Anaerobic digestion (AD) is a sustainable waste management technology that primarily generates two products: biogas and digestate. The technology relies on the microorganisms' activity, which depends on several operational factors, such as pH, temperature, solid contents, and ammonia levels. Ammonia is an inorganic form of nitrogen resulting from the biodegradation of organic nitrogen. It is considered one of the major concerns for AD operations due to its inhibitory effects on some microorganisms, particularly methanogens. A common feedstock characterized by high nitrogen content is poultry manure (PM). PM is often avoided in anaerobic digesters due to the anticipated inhibition resulting from its high ammonia levels. However, since poultry manure is one of the most widely available organic wastes, researchers have extensively investigated ways to include PM as a primary feedstock for AD. One possible way to treat high ammonia levels in digestate is ammonia stripping, the physio-chemical separation of ammonia from a solution by introducing a stripping (carrier) gas. There are a few approaches to performing ammonia stripping in AD applications; the most commonly discussed in the literature are pre-hydrolysis and side-stream ammonia stripping. Pre-hydrolysis ammonia stripping is performed on raw feedstock after increasing pH and temperature and is usually not restricted in selecting the gas carrier. On the other hand, side-stream ammonia stripping is when a portion of the digester's working volume is filtered, and the filtrate is sent to a unit where pH and temperature are increased. The carrier gas in these systems is often limited to anaerobic gases such as biogas or steam. The third and most novel approach is post-hydrolysis ammonia stripping, conducted at an intermediate stage between hydrolysis and methanogenesis in a two-stage AD process. This configuration would address the shortcomings of the other two systems. However, there is minimal information on the feasibility and potential of this approach in the literature. This study aims to comprehensively investigate the post-hydrolysis ammonia stripping approach through the following four phases: Phase I) Proof of Concept; Phase II) Optimization; Phase III) Assessment of Alternative Carrier Gases; and Phase IV: Comparison of Different Ammonia Stripping Configurations. Phase I provided the proof of concept under the batch mode and compared the performance of post-hydrolysis ammonia stripping with two-stage AD and co-digestion to improve poultry manure's methane potential as the primary substrate. It was observed that ammonia stripping successfully improved methane potential by up to 150%, whereas improvements due to two-stage AD and co-digestion were limited to 41 and 9%, respectively. Phase II provided more insight into optimizing the ammonia stripping process. Different stripping conditions were tested (pH 7.8 (unadjusted), 9 and 10, temperature 25 (unadjusted), 40 and 55 °C, and flow rate 300 L/L/hour). The results showed that higher pH and temperature lead to higher removal efficiency. However, it was concluded that optimal conditions ultimately depend on the initial and target ammonia levels. Moreover, Analysis of Variance showed that pH and temperature were significant factors affecting the ammonia removal efficiency. In addition, it was observed that higher stripping temperatures (55 °C) enhanced the digestibility of PM and increased its methane potential more than stripping at 40 °C. It was concluded that the optimum stripping conditions were pH 9.5, temperature 40 or 55 °C, and flowrate of 100 L/L/hour to collectively increase ammonia removal while reducing the associated costs and material handling. In Phase III, renewable natural gas (RNG) was evaluated as a stripping medium in batch testing as a potential replacement for biogas and air. Ammonia stripping with RNG yielded promising results comparable to the application of air in terms of ammonia removal and enhancing biogas production from PM (60 and 69% ammonia removal for RNG and air, respectively). In addition, a metagenomic shotgun analysis showed that most biogas production was conducted through hydrogenotrophic methanogens instead of acetoclastic methanogens, which are more susceptible to high ammonia levels. Phase IV assessed the semi-continuous flow two-stage operation of mesophilic AD reactors coupled with different ammonia stripping configurations. Post-hydrolysis ammonia stripping successfully achieved a stable operation of PM mono-digestion at ammonia levels of 1700 and 2400 mg NH₃-N/L in the cases of stripping with air and RNG, respectively. In addition, post-hydrolysis ammonia stripping in semi-continuous flow mode may have promoted acetoclastic methanogens growth since volatile fatty acid concentrations were reduced in the digesters. Phase IV also proved that the performance of post-hydrolysis ammonia stripping is superior over pre-hydrolysis and side-stream ammonia stripping. In the semi-continuous flow reactors, post-hydrolysis ammonia stripping with air achieved on average 831 L biogas/ kg VS at an organic loading rate (OLR) of 2.6 g VS/L/day, whereas side-stream ammonia stripping resulted in average of 700 L biogas/ kg VS at OLR of 1.8 g VS/L/day and higher ammonia stripping requirements. Having said that, the base scenario (no ammonia stripping) was inhibited, indicating that both ammonia stripping configurations were considered successful in alleviating inhibitory effects of ammonia from poultry manure. Phase IV results also proved that air stripping repeatedly outperformed RNG as stripping mediums by having higher ammonia removal efficiencies resulting in higher methane production. However, stripping with RNG is believed to have more practical advantages than air due to avoiding the risk of oxygen infiltration into the reactor. Moreover, renewable natural gas has proven to be an efficient stripping medium that is available on-site. The final stage of Phase IV tested pre-hydrolysis ammonia stripping using air in batch mode and compared it with post-hydrolysis ammonia stripping. Pre-hydrolysis ammonia stripping provided little to no improvement to the methane potential of PM in batch mode and therefore was excluded from the semi-continuous flow experiment. The four phases of this study demonstrated the flexibility and the superiority of post-hydrolysis ammonia stripping over the other pre-hydrolysis and side-stream ammonia stripping. In addition, post-hydrolysis ammonia stripping was proven efficient and feasible for ammonia removal and enabling the mono- or co-digestion of poultry manure. The study also showed that using RNG instead of biogas can significantly reduce the operational costs of the treatment.

Anaerobic digestion is a process where biogas is generated from organic substance in the absence of oxygen. The most common application of the anaerobic digestion technology in developing countries is small-scale household digesters producing biogas for cooking purposes. These systems are usually fed with cattle dung or organic household waste. The typical small-scale digester models are fixed-dome digester, floating-drum digester and rubber-balloon digester. Biogas systems contribute to self-sustainability of energy production, improve waste management and mitigate deforestation and health problems caused by poor waste management and usage of traditional cooking fuels such as firewood and charcoal. Anaerobic digestion technology is still quite unknown in Mozambique. An initiative to implement this technology in the municipality of Quelimane located in Zambézia province in the central part of the country, was taken in 2015 and background studies were performed during spring 2015 and 2016. As a part of the study resulting in this report, performed in spring 2017, a small-scale biogas digester was installed in Quelimane city. The digester was fed with the initial input of cow manure in order to create population of anaerobic bacteria and in the future it’s planned to be fed with food waste. The purpose of the first installed digester is to serve for educational purposes and to produce cooking fuel for the few people working in the municipal location where the digester is installed. It’s estimated that the digester has a potential to produce daily 0.3 m3 of biogas from 2 kg of food waste which can be used for preparing lunch for the employees or for heating water. In the future, possible applications of anaerobic digestion technology in Quelimane are a municipal biogas plant, cooling systems for the fish industry and biogas based latrines in the less developed areas located outside of the city center.

About 83% of total energy consumed in Nepal comes directly from the solid fuels. The import of Liquified Petroleum Gas (LPG) is also growing at an exponential rate. The hospitality subsector (hotels and restaurants), one of the key economic subsectors in the country, consumes most of the energy within the commercial sector primarily for cooking purposes. The bio-waste generated from growing hospitality subsector and other sectors in Kathmandu is poorly managed. Similarly, on the other hand, Nepal has an extensive knowledge and experience of manure based anaerobic household biogas systems mainly in rural areas. Based upon this situation, the thesis investigates the opportunities for anaerobic biogas production for cooking at hotels and restaurants by utilizing their own organic waste. Currently available biogas technologies, important parameters affecting the biogas yield, policy and financial supports and case studies of various stakeholders employing the biogas technologies in the hotels and restaurants in Nepal were considered. The most applicable technology for the purpose was then chosen. The organic waste sampling study from randomly selected 4-star hotel (Yatri Spa and Suites), tourist standard hotel (Hotel Bliss International) and restaurant (Fren’s Kitchen) in Thamel, the tourist zone of Kathmandu was conducted. Similarly, various data especially focusing on the current cooking energy need, demand, supply, cost, organic waste management of the hotels and the restaurant was collected through questionnaires and series of interviews. The average amount of daily organic waste and organic waste fraction for Yatri, Bliss and Fren’s was found to be 61.3 kg and 63.0%, 18.4 kg and 82.7%, and 16.3 kg and 81.8% respectively. Similarly, the variations in weekly organic wastes and waste generated per guest was also determined. Based upon the amount and characteristics of organic waste from the sampling survey, the theoretical biogas potential of the organic waste at digester output rate of 0.27 kWh/kg/day for JUAS digesters, the technology selected for the biogas conversion, was found to be 18.4 kWh/day, 5.5 kWh/day and 4.9 kWh/day equivalent to 4.9%, 3.0% and 5.4 % of the current daily energy need for Yatri, Bliss and Fren’s respectively. Similarly, the economic implications of the small-scale biogas technology if employed in the hotels and the restaurant was scoped out. It was found that the recommended Polyvinyl Chloride (PVC) based 3000 l sized JUAS bio-digesters had positive Net Present Value (NPV), Internal Rate of Return (IRR) and payback time of around 5 years on average for all the eateries under study. The monthly life cycle cost of the integrated LPG-JUAS system is found be cheaper for all eateries as compared to the current LPG system. Levelized Cost of Energy (LCoE) of the JUAS digesters is calculated to be competitive as compared to energy from other renewables in the country. There is, however, need to improve the digester conditions to get higher biogas yields. For the wider adoption of the digesters across urban sectors, the subsidies amount should also be increased together with information dissemination regarding biogas uses and its potential among the stakeholders. / Omkring 83% av den totala energiförbrukningen i Nepal kommer direkt från det fasta bränslet. Importen av flytande petroleumgas (LPG) växer också i exponentiell takt. Underhållningsbranschen (hotell och restauranger), en av de viktigaste ekonomiska delområdena i landet, förbrukar mest energi inom kommersiell sektor, främst för matlagning. Det biologiska avfallet som genereras av växande underhållsbranschen och andra sektorer i Katmandu är dåligt förvaltad. På samma sätt har Nepal en omfattande kunskap och erfarenhet av gödselbaserade anaeroba hushållsbiogasystem, huvudsakligen i landsbygdsområden. Baserat på denna situation undersöker man avhandlingen möjligheterna till anaerob biogasproduktion för att laga mat på hotell och restauranger genom att använda eget organiskt avfall. För närvarande finns biogasteknik, viktiga parametrar som påverkar biogasutbytet, politiska och finansiella stöd och fallstudier av olika intressenter som använder biogasteknik i hotell och restauranger i Nepal. Den mest tillämpliga tekniken för ändamålet valdes sedan. Undersökning av organiska avfallsprov från slumpmässigt utvalt 4-stjärnigt hotell (Yatri Spa and Suites), turisthotell (Hotel Bliss International) och restaurang (Fren's Kitchen) i Thamel, utförs turistområdet Katmandu. På samma sätt samlades olika data som speciellt fokuserade på dagens energibehov, efterfrågan, tillgång, kostnad, organisk avfallshantering av hotellen och restaurangen genom enkäter och intervjuer. Den genomsnittliga mängden dagligt organiskt avfall och organiskt avfallsfraktion för Yatri, Bliss och Fren var visat sig 61,3 kg respektive 63,0%, 18,4 kg respektive 82,7% respektive 16,3 kg respektive 81,8%. På samma sätt bestämdes också variationerna i organiskt avfall per vecka och avfall som genererades per gäst. Baserat på mängden och egenskaperna hos organiskt avfall från provtagningsundersökningen befanns den teoretiska biogaspotentialen hos det organiska avfallet vid kokareutgångshastigheten på 0,27 kWh / kg / dag för JUAS-kokare, den teknik som valts för biogasomvandling, befunnits vara 18,4 kWh / dag, 5,5 kWh / dag och 4,9 kWh / dag motsvarande 4,9%, 3,0% och 5,4% av dagens energibehov för Yatri, Bliss och Fren. På samma sätt scenkades de ekonomiska konsekvenserna av den småskaliga biogastekniken om de anställdes i hotell och restaurangen. Det visade sig att de rekommenderade polyvinylkloridbaserade (PVC) -baserade JUAS-bioförstörare av polyvinylklorid med jämna mellanrum hade positivt nettoförsäkringsvärde (NPV), interna avkastningsräntor och återbetalningstid på cirka 5 år i genomsnitt för alla restauranger som studerades. Den månatliga livscykelkostnaden för det integrerade LPG-JUAS-systemet befinner sig vara billigare för alla matställen jämfört med det aktuella LPG-systemet. Nivånad kostnad för energi (LCoE) hos JUAS-kokare beräknas vara konkurrenskraftig jämfört med energi från andra förnybara energikällor i landet. Det är dock nödvändigt att förbättra kokareförhållandena för att få högre biogasutbyten. För det bredare godkännandet av kokare i städerna bör bidragsbeloppet också ökas tillsammans med informationsspridning avseende biogasanvändning och dess potential bland intressenterna.För det bredare godkännandet av kokare i städerna bör bidragsbeloppet också ökas tillsammans med informationsspridning avseende biogasanvändning och dess potential bland intressenterna.

This study has been carried out within the framework of the Minor Field Studies ScholarshipProgramme (MFS), which is funded by the Swedish International Development Cooperation Agency,Sida. The field study was conducted in Grenada in the spring of 2023. Grenada, along with severalcountries in the Caribbean, has under the last couple of years experienced an increasing influx ofSargassum seaweed. The seaweed accumulates on beaches and causes complications relating tohealth, recreation, economy and the ecosystems by the coast. Moreover, Grenada is dependent onimported fossil fuels which causes the country to have a relatively high ecological footprint as well asmaking the country sensitive to volatile oil prices. To produce biogas with Sargassum, and morespecifically for generating electricity, is one possible solution to the aforementioned issues. However,there is a need to investigate the prerequisites for such a solution. This was done by conducting anexperiment and a market analysis. The experiment aimed to investigate how the methane content inthe biogas is affected by mechanical pretreatment of the seaweed. The market analysis delved into thepossibilities of using biogas to generate electricity in Grenada. For the experiment, a small-scale biogas digester was used, which was located at the True Blue BayBoutique Resort. Feeding of the digester was divided into three batches with two differentcompositions of food waste and Sargassum; untreated and mechanically pretreated Sargassum. Thedigester was fed for 42 days in total while the measurements were conducted for 28 days. In terms ofthe market analysis, a literature study and 4 interviews with relevant actors were conducted. Theinterviewees were approached based on local suggestions. For the analysis, four different areas ofinterest were selected: Electricity system in Grenada, Policy and regulation, Infrastructure andAvailability of organic waste for co-digestion. The experiment revealed a weak yet positive trend in the methane yield over the measured period,indicating that mechanical pretreatment of the Sargassum could potentially increase methane yields.Specifically, the average methane concentration by volume was 57% for the untreated batch and 63%for the mechanically pretreated batch. However, it is important to note that the project's duration wasinsufficient to draw definitive conclusions. Furthermore, there might be other factors that contributedto the unusually high methane yield observed in this experiment. Firstly, the storage method of theseaweed in fresh saltwater may have played a role. Secondly, uncertainties related to gas fluxmeasurements and estimated thermal efficiency have a significant impact on the calculated final valueof the methane concentration. In terms of the market analysis, the results indicate that electricity generation through large-scalebiogas production will face challenges. The structure of the electricity system in Grenada allows forgeneration from biogas and Sargassum to exist. Moreover, such operations can play a significant rolein realizing the goals set forth by Grenada’s main policies in terms of sustainability and energy.However, the challenges that need to be dealt with include establishing regulations and policiesspecifically targeting biogas and Sargassum. Supporting infrastructure and a reliable source ofbiodegradable waste also needs to be established before electricity generation fromSargassum-derived biogas can be carried out. The aforementioned challenges relate to electricityproduction for distribution and may not be as significant for power generation for self-consumption. / Denna studie är genomförd inom ramen för stipendieprogrammet Minor Field Studies (MFS) somfinansieras av Sida och fältstudien genomfördes i Grenada våren 2023. Grenada, tillsammans medflera länder i Karibien, har under de senaste åren upplevt ett ökande inflöde av Sargassotång. Tångensamlas på stränderna och skapar problem kopplade till hälsa, rekreation, ekonomi och ekosystemenvid kusten. Vidare är Grenada beroende av importerade fossila bränslen, vilket gör att landet har ettrelativt högt ekologiskt fotavtryck, samtidigt som det är känsligt för volatila oljepriser. Att producerabiogas med sargassotång, och mer specifikt för att generera el, är en möjlig lösning till de ovannämnda problemen. Emellertid finns det ett behov att undersöka förutsättningarna för en sådanlösning. Detta gjordes genom att genomföra ett experiment och en marknadsanalys. Experimentetsyftade till att undersöka hur metaninnehållet påverkas av att mekaniskt förbehandla sargassotången.Marknadsanalysen undersökte förutsättningarna för att använda biogasen för att generera el påGrenada. För experimenten användes en småskalig biogasanläggning på True Blue Bay Boutique Resort påGrenada. Matningen delades upp i tre delar med två olika sammansättningar av matavfall ochsargassotång; obehandlade och mekaniskt förbehandlade alger. Totalt matades anläggningen i 42dagar medan experimentet med vattenkokning genomfördes 28 dagar. För marknadsanalysengenomfördes en litteraturstudie och 4 intervjuer med relevanta aktörer. Intervjupersonerna valdesutifrån lokala förslag. För analysen valdes sedan fyra områden: Elsystemet på Grenada, Policy ochreglering, Infrastruktur och Tillgänglighet av biologiskt nedbrytbart avfall. Experimentet visade en svag men positiv trend i metanhalten under den uppmätta perioden, vilkettyder på att mekanisk förbehandling av Sargassum potentiellt kan öka metanhalten. Mer specifikt varden genomsnittliga koncentrationen av metan per volym 57% för den obehandlade delen och 63% förden mekaniskt förbehandlade delen. Det är dock viktigt att notera att projektets varaktighet varotillräcklig för att dra definitiva slutsatser. Dessutom kan det finnas andra faktorer som bidrog till detovanligt höga metanhalten som observerades i detta experiment. För det första kan lagringsmetodenav sjögräset i färskt saltvatten ha spelat en roll. För det andra har osäkerheter relaterade tillgasflödesmätningar och uppskattad verkningsgrad en betydande påverkan på den beräknade slutligametankoncentrationen. Vad gäller marknadsanalysen så indikerar resultaten att elproduktion genom storskaligbiogasproduktion kommer att möta utmaningar. Utformningen av Grenadas elsystem tillåtergeneration av el från biogas och Sargassum. Vidare kan en sådan verksamhet spela en viktig roll i attuppfylla de mål som presenteras i Grenadas huvudsakliga strategier kring hållbarhet och energi.Emellertid behövs regler och policy som är specifikt inriktade på biogas och Sargassum. Därtillbehövs stödjande infrastruktur och en tillförlitlig ström av biologiskt nedbrytbart avfall. De nämndautmaningarna gäller främst för elproduktion för distribution och behöver inte vara lika signifikanta förkraftproduktion för egenkonsumtion.

More than 16,000 tons of olive cake was produced in the United States in 2017. Olive cake is a by-product of olive oil extraction, which has limited animal feed potential, and poses an environmental threat when landfilled due to its high organic load and polyphenol content. This residue has potential for biofuel (bioethanol and biomethane) production because it is rich in polysaccharides such as pectin, hemicellulose, and cellulose. Yet, olive cake contains olive stones that can impede its conversion to biofuel. Therefore, two methods of destoning, centrifugation and screening by horizontal screw press, were first compared. Both methods removed an equal percentage of stones (95%), but centrifugation partitioned the majority (57 – 79%) of digestible solids (olive pulp) with the stones. Then, two strategies were compared to maximize both biomethane and bioethanol production; enzymatic conversion of insoluble to soluble carbohydrates and destoning by screening. After 30 days of anaerobic digestion at 35 °C, both the enzymatically pretreated and the destoned olive cakes produced similar amounts of methane (~295 mL CH4/g VS), 42% more than the control (209.5 mL CH4/g VS). The biogas produced was composed of 60-70% methane. A comparison of biomethane yields with a broad range of agricultural residues demonstrated olive cake’s suitability for biomethane production. The digestate, residue from the anaerobic digestion, have high Kjeldahl nitrogen content (3.6%, db) and low polyphenol concentration (0.02 mg GAE/g), which then qualify it as an ingredient for soil amendment. Ethanol production investigations showed that after 3 days of fermentation at 32 °C, only the destoned and enzymatically pretreated olive cake produced ethanol (1.3 mg/mL). Acetic acid, an inhibitor of ethanol production, was present in all samples broth, suggesting microbial contamination was present. These results provide evidence that olive cake can be diverted from landfills to be converted into a biofuel. Sustainable pretreatments such as destoning and enzymatic pretreatment increase biomethane yield. The digestate created from the anaerobic digestion of olive cake can be used as a soil amendment, adding further value to olive cake.