This study devised a method to detect Ribeiroia ondatrae (class Trematoda) in water-bodies using environmental DNA (eDNA) collected from filtered water samples from selected ponds in the USA and Canada. Species-specific PCR primers were designed to target the Internal Transcribed Spacer-2 (ITS-2) region of the parasite’s genome. The qualitative PCR method was 70% (n=10) accurate in detecting R. ondatrae in ponds previously found to contain the parasite, while the qPCR method was 88.9% (n=9). To examine how the retinoic acid (RA) pathway gene expression may be perturbed during R. ondatrae infections, leading to limb development abnormalities in the wood frog (Lithobates sylvaticus). Multiple sequence alignments were used to design degenerate PCR primers to eight RA biosynthesis genes, but only two gene fragments were identified using this approach. Without effective primer sets it was not possible to measure changes in gene expression in infected frogs.

This study devised a method to detect Ribeiroia ondatrae (class Trematoda) in water-bodies using environmental DNA (eDNA) collected from filtered water samples from selected ponds in the USA and Canada. Species-specific PCR primers were designed to target the Internal Transcribed Spacer-2 (ITS-2) region of the parasite’s genome. The qualitative PCR method was 70% (n=10) accurate in detecting R. ondatrae in ponds previously found to contain the parasite, while the qPCR method was 88.9% (n=9). To examine how the retinoic acid (RA) pathway gene expression may be perturbed during R. ondatrae infections, leading to limb development abnormalities in the wood frog (Lithobates sylvaticus). Multiple sequence alignments were used to design degenerate PCR primers to eight RA biosynthesis genes, but only two gene fragments were identified using this approach. Without effective primer sets it was not possible to measure changes in gene expression in infected frogs.

The writing style of a number of authors writing in English was empirically investigated for the purpose of detecting stylistic patterns in relation to advancing age. The aim was to identify the type of stylistic markers among lexical, syntactical, phonemic, entropic, character-based, and content ones that would be most able to discriminate between early, middle, and late works of the selected authors, and the best classification or prediction algorithm most suited for this task. Two pilot studies were initially conducted. The first one concentrated on Christina Georgina Rossetti and Edgar Allan Poe from whom personal letters and poetry were selected as the genres of study, along with a limited selection of variables. Results suggested that authors and genre vary inconsistently. The second pilot study was based on Shakespeare's plays using a wider selection of variables to assess their discriminating power in relation to a past study. It was observed that the selected variables were of satisfactory predictive power, hence judged suitable for the task. Subsequently, four experiments were conducted using the variables tested in the second pilot study and personal correspondence and poetry from two additional authors, Edna St Vincent Millay and William Butler Yeats. Stepwise multiple linear regression and regression trees were selected to deal with the first two prediction experiments, and ordinal logistic regression and artificial neural networks for two classification experiments. The first experiment revealed inconsistency in accuracy of prediction and total number of variables in the final models affected by differences in authorship and genre. The second experiment revealed inconsistencies for the same factors in terms of accuracy only. The third experiment showed total number of variables in the model and error in the final model to be affected in various degrees by authorship, genre, different variable types and order in which the variables had been calculated. The last experiment had all measurements affected by the four factors. Examination of whether differences in method within each task play an important part revealed significant influences of method, authorship, and genre for the prediction problems, whereas all factors including method and various interactions dominated in the classification problems. Given the current data and methods used, as well as the results obtained, generalizable conclusions for the wider author population have been avoided.

No description available.

Recent research has revealed human settlement on the Pacific coast of Canada extending back nearly 14,000 years, but much of the late Pleistocene record is unknown due to shifting sea levels, poor understanding of Cordilleran ice extent, and limited research on the biota of the coast during this time. This study, undertaken in Quatsino First Nation and ‘Namgis First Nation territories as part of the Northern Vancouver Island Archaeology and Palaeoecology Project, employs modern multi-proxy analysis of lake sediment cores from two sites on northern Vancouver Island to reconstruct palaeoenvironments during and immediately following the Fraser Glaciation in coastal British Columbia. Evidence from radiocarbon samples, pollen, ancient environmental DNA, plant macrofossils, and diatoms indicates that Topknot Lake on the outer coast of Vancouver Island has remained unglaciated through most of the local Last Glacial Maximum since ca. 18,000 cal BP. A non-arboreal herb-shrub tundra assemblage prevailed from ca. 17,500-16,000 cal BP with taxa including willows (Salix), grasses, sedges (Cyperaceae), heathers (Ericaceae), and sagewort (Artemisia). After ca. 16,000 and into the terminal Pleistocene, Topknot Lake was dominated by pine, alder (Alnus), ferns, and aquatic plant species. In the Nimpkish River Valley deep in the Vancouver Island Ranges, Little Woss Lake also demonstrates a record extending to the late Pleistocene (ca. 14,300 cal BP). The environment comprised dry and cool conifer woodland dominated first by fir (Abies) until ca. 14,000 cal BP, then by pine, alder, and ferns from ca. 14,000-12,000 cal BP. eDNA evidence from ca. 14,000 cal BP corroborates these plant taxa as well as indicating brown bear and Chinook salmon in and around the basin at that time. A mixed-conifer assemblage consisting of pine, western hemlock, and alder followed from ca. 12,000-11,100 cal BP into the early Holocene. Collectively, these indicators demonstrate an open environment on the outer coast of northern Vancouver Island since ca. 18,000-17,500 cal BP and well-established biotic communities across the region throughout the late Pleistocene. These results inform future archaeological research for early human habitation in coastal British Columbia and provide key evidence to support the viability of the coastal migration route for the first peopling of the Americas. / Graduate / 2020-12-11

Edna O'Brien and Marian Keyes are two sexually candid female writers. They are both from Ireland and their novels have caused many spectacular headlines over the years. These female authors have been greatly acclaimed, but also sharply criticised for their outspoken way of writing. However they have enjoyed huge success and their novels have been read by many people. This essay will focus on two of the many novels written by these Irish authors: The Country Girls by Edna O'Brien and Anybody Out There? by Marian Keyes. In the following essay I will focus on Irish women represented in the novels of Keyes and O'Brien, and their relation to men but also to their daughters. In these novels, women are forced into strict gender roles, very different from those of men. Still, even though the older women in the novels are trapped in their roles, they encourage their daughters to develop and grow beyond such roles. I would argue that the novels and also the feminist actions that still occur in Ireland are built upon a long time of subordination for women, and also from the twisted structure in many of the Irish men-women-relationships. I would argue that the rules and roles created in historical Ireland, have formed an inequality between the sexes that still exists.

No description available.

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第22078号 / 農博第2370号 / 新制||農||1072(附属図書館) / 学位論文||R1||N5232(農学部図書室) / 京都大学大学院農学研究科応用生物科学専攻 / (主査)教授 山下 洋, 教授 吉田 天士, 准教授 益田 玲爾 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

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Aquatic invasive species are a major threat to native freshwater biodiversity. The North American signal crayfish Pacifastacus leniusculus was introduced to Great Britain during the 1970s and is now widely distributed throughout England, Wales and Scotland. First recorded in Scotland in 1995, P. leniusculus is now established at more than twenty sites. The only other introduced crayfish species present in Scotland is the white-clawed crayfish Austropotamobius pallipes. A. pallipes is restricted to only two locations in Scotland, Loch Croispol and Whitemoss Reservoir. P. leniusculus negatively impacts macrophytes, invertebrates and fish though ecological and physical processes. Additionally, P. leniusculus has displaced A. pallipes throughout much of its native range within Great Britain due to competition and disease. Consequently, the two A. pallipes populations in Scotland have a high conservation value. This PhD study aimed to improve understanding of P. leniusculus invasion success by examining trophic dynamics and to develop methodologies that could improve the detection and control of P. leniusculus populations in Scotland. Stable isotope analysis was used to determine the diet composition, trophic position and whether an ontogenetic dietary shift occurs in the Loch Ken population of P. leniusculus. Bayesian mixing models indicated that P. leniusculus in Loch Ken do exhibit an ontogenetic dietary shift. Additionally, individuals of all sizes occupied the trophic position of a predator in Loch Ken suggesting that invertebrates and fish constitute an important component of P. leniusculus diet. Stable isotope analysis was used once again to compare the isotopic niche width and diet composition of P. leniusculus populations from Loch Ken and A. pallipes populations from Loch Croispol and Whitemoss Reservoir. At the species level, A. pallipes exhibited a larger niche width than that of P. leniusculus. At the population level, the isotopic signatures of the A. pallipes populations were considerably different from each other suggesting an overestimation of A. pallipes’ niche width at species level. Results showed no dietary overlap between species and Bayesian mixing models suggested P. leniusculus and A. pallipes were consuming different resources, indicating there would be no direct competition for food resources if they were to co-occur. A plus-maze study was used to determine if P. leniusculus exhibited a preference for one of four food attractants (Oncorhynchus mykiss, P. leniusculus, beef or vegetation), which could be used to improve trapping efficiency. In the maze system, P. leniusculus exhibited no preference for any food attractant presented. This would suggest that either the maze was not a good model or food attractants would not improve trapping efficiency of P. leniusculus. Additionally, a comparative investigation into the use of gill nets as a method to control P. leniusculus was conducted. Results showed that the net type and the presence of fish entangled in the net influenced the number of P. leniusculus caught. Finally, environmental DNA (eDNA) was used and evaluated for detection of P. leniusculus. A robust quantitative Polymerase Chain Reaction (qPCR) assay and DNA extraction protocol were developed. Using the developed qPCR assay, P. leniusculus eDNA was detected in controlled aquaria conditions but not in environmental water samples collected from the field. Furthermore, the quantities of P. leniusculus eDNA declined in aquaria conditions while individuals were still present suggesting the mechanisms for eDNA release by P. leniusculus are complex. Stable isotope analysis indicates that P. leniusculus exhibit an ontogenetic dietary shift, and in each life stage, P. leniusculus function as an omnivore but occupy the trophic position of a predator. Niche width analysis revealed that the diet of P. leniusculus was less general than that observed in A. pallipes and thus diet of P. leniusculus may not be responsible for invasive success. Food attractants will not enhance trapping efficiency but nets may present a potential new method to control P. leniusculus. Similarly, eDNA presents a promising new method for rapid detection of P. leniusculus. It will not be possible to eradicate P. leniusculus in Scotland but the findings of this PhD may help prevent establishment of new populations. These results should be incorporated into future management strategies for P. leniusculus populations in Scotland and may have broader applications in Great Britain and Europe.