In this work, the identification of the promoter for c-cytochromes Cyt c Id-1 in Ideonella dechloratans has been investigated. Additionally, the study examined investigating whether gene expression is affected by the presence and absence of oxygen. This was investigated by amplifying the promoter region of Cyt c Id-1 (389 bp) and then cloning it into a reporter vector lacking a functional promoter for the upstream gene β-galactosidase. The reporter vector was transformed into E. coli RM101 and grown under both aerobic and anaerobic conditions. To examine the gene expression of Cyt c Id-1 the activity of β-galactosidase has been measured in both the aerobic and anaerobic cultures. The result showed that the gene is induced more in an anaerobic environment than in an aerobic environment, by comparing the activity of β-galactosidase under aerobic and anaerobic conditions. / I detta arbete har identifiering av promotorn för c-cytokromer Cyt c Id-1 i Ideonella dechloratans undersökts, samt att undersöka om genuttrycket påverkas av närvaro och frånvaro av syre. Detta är undersökts genom att amplifiera promotorregionen för Cyt c Id-1 (389 bp) för att sedan klona in den in i en reportervektor som saknar en fungerande promotor för uppströms genen β-galaktosidas. Reportervektorn transformerades till E. coli RM101 och odlades under både aeroba och anaeroba förhållanden.  För att undersöka genuttrycket av Cyt c Id-1 har aktiviteten hos β-galaktosidas uppmätts i både de aeroba och anaeroba odlingarna. Resultatet visat att genen induceras mer i anaerob miljö än i aerob miljö, genom att jämföra aktiviteten av β-galaktosidas under både aerob och anaerob förhållande.

In this work, an electrochemical method based on a cyt c biosensor has been developed, for the detection of selected arsenic and cyanide compounds. Boron Doped Diamond (BDD) electrode was used as a transducer, onto which cyt c was immobilised and used for direct determination of Prussian blue, potassium cyanide and arsenic trioxide by inhibition mechanism. The sensitivity as calculated from cyclic voltammetry (CV) and square wave voltammetry (SWV), for each analyte in phosphate buffer (pH= 7) was found to be (1.087- 4.488 ×10-9 M) and the detection limits ranging from 0.0043- 9.1 μM. These values represent a big improvement over the current Environmental Protection Agency (EPA) and World Health Organisation (WHO) guidelines.

In this work, an electrochemical method based on a cyt c biosensor has been developed, for the detection of selected arsenic and cyanide compounds. Boron Doped Diamond (BDD) electrode was used as a transducer, onto which cyt c was immobilised and used for direct determination of Prussian blue, potassium cyanide and arsenic trioxide by inhibition mechanism. The sensitivity as calculated from cyclic voltammetry (CV) and square wave voltammetry (SWV), for each analyte in phosphate buffer (pH= 7) was found to be (1.087- 4.488 ×10-9 M) and the detection limits ranging from 0.0043- 9.1 μM. These values represent a big improvement over the current Environmental Protection Agency (EPA) and World Health Organisation (WHO) guidelines.

Magister Scientiae - MSc / In this work, an electrochemical method based on a cyt c biosensor has been developed, for the detection of selected arsenic and cyanide compounds. Boron Doped Diamond (BDD) electrode was used as a transducer, onto which cyt c was immobilised and used for direct determination of Prussian blue, potassium cyanide and arsenic trioxide by inhibition mechanism. The sensitivity as calculated from cyclic voltammetry (CV) and square wave voltammetry (SWV), for each analyte in phosphate buffer (pH= 7) was found to be (1.087- 4.488 ×10-9 M) and the detection limits ranging from 0.0043- 9.1 μM. These values represent a big improvement over the current Environmental Protection Agency (EPA) and World Health Organisation (WHO) guidelines. / South Africa

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