A tecnologia da microencapsulação apresenta várias aplicações na indústria de alimentos. Sabendo-se que diferentes fatores intrínsecos e extrínsecos dos alimentos podem influenciar a produção e atividade antimicrobiana das bacteriocinas produzidas pelas bactérias láticas, este estudo teve como principal objetivo avaliar a funcionalidade da encapsulação de bactérias láticas (BAL) bacteriocinogênicas em alginato de cálcio no controle de Listeria monocytogenes em salame experimentalmente contaminado. Para atingir este objetivo, foram isoladas novas cepas de BAL a partir de salame, que foram identificadas e caracterizadas quanto às propriedades das bacteriocinas produzidas, avaliando-se a influência do processo de encapsulação na produção de bacteriocinas. Foram isoladas quatro cepas produtoras de bacteriocinas, identificadas como Lactobacillus sakei (uma cepa), Lactobacillus curvatus (duas cepas) e Lactobacillus plantarum (uma cepa), nomeadas MBSa1, MBSa2, MBSa3 e MBSa4, respectivamente. As bacteriocinas produzidas pelas quatro cepas foram termoestáveis e com exceção da cepa MBSa2, sensíveis a pH acima de 8. Todas inibiram todas as cepas de Listeria monocytogenes testadas e várias espécies de BAL, mas foram inativas contra bactérias Gram negativas. As bacteriocinas foram purificadas por cromatografia de troca iônica seguida de cromatografia de interação hidrofóbica sequencial e cromatografia de fase reversa, observando-se que L. sakei MBSa1 produz um peptídeo de 4303 Da, com uma sequência parcial de aminoacidos idêntica à sequência presente em sakacina A. As cepas MBSa2 e MBSa3 produzem dois peptídeos ativos cada, idênticos nas duas cepas, um de 4457 Da e outro de 4360 Da, que apresentam sequências parciais idênticas às presentes na sakacina P e na sakacina X, respectivamente. Aparentemente, a cepa L. plantarum MBSa4 produz uma bacteriocina composta por duas sub-unidades. O DNA genômico da cepa L. sakei MBSa1 contém os genes da sakacina A e curvacina A, enquanto o DNA da cepa L. plantarum MBSa4 foi positivo para o gene da plantaricina W. A cepa L. curvatus MBSa2 foi encapsulada em alginato de cálcio e testada quanto à produção de bacteriocinas in vitro, observando-se que o processo de encapsulação não influenciou a produção de bacteriocina. Quando testada in situ, ou seja, no salame experimentalmente contaminado com Listeria monocytogenes, não foi observada ação anti-Listeria por L. curvatus MBSa2 encapsulado e não encapsulado, durante o 30 dias de fabricação do salame. / The microencapsulation technology has several applications in the food industry. Knowing that different intrinsic and extrinsic factors can influence production and antimicrobial activity of bacteriocins produced by lactic acid bacteria in foods, this study aimed at evaluating the functionality of the encapsulation of bacteriocinogenic lactic acid bacteria (LAB) in calcium alginate in the control of Listeria monocytogenes in experimentally contaminated salami. To achieve this goal, new strains of LAB were isolated from salami, identified and characterized for the properties of the produced bacteriocins, evaluating the influence of the encapsulation process in the bacteriocins production. Four bacteriocin producing strains were isolated and identified as Lactobacillus sakei (one strain), Lactobacillus curvatus (two strains) and Lactobacillus plantarum (one strain), named MBSa1, MBSa2, MBSa3 and MBSa4 respectively. The bacteriocins produced by the four strains were thermostable and with the exception of strain MBSa2, sensitive to pH above 8. All inhibited all tested Listeria monocytogenes strains and various species of LAB but were inactive against Gram-negative bacteria. The bacteriocins were purified by cation-exchange followed by sequential hydrophobic-interaction and reversed-phase chromatography, indicating that L. sakei MBSa1 produces a peptide of 4303 Da, with a partial amino acid sequence identical to the sequence present in sakacin A. L. curvatus MBSa2 and MBSa3 produce two active peptides, identical in the two strains, one of 4457 Da and the other of 4360 Da, with partial aminoacid sequences identical to those present in sakacin X and sakacin P, respectively. Apparently, L. plantarum MBSa4 produces a bacteriocin composed of two subunits. Genomic DNA of L. sakei MBSa1indicated that this strain contains genes for sakacin A and curvacin A, while the DNA of L. plantarum MBSa4 was positive for the plantaricin W gene. The strain L. curvatus MBSa2 was encapsulated in calcium alginate and tested for bacteriocin production in vitro, observing that the encapsulation process did not affect the production of bacteriocin. When tested in situ, i.e. in the salami experimentally contaminated with L. monocytogenes was not observed anti-Listeria<i/> action by L. curvatus MBSa2 encapsulated and non-encapsulated during the 30 day manufacture of salami.

Since the acknowledgement of the entrapment defense by the Supreme Court in the case of Sorrells v. United States 287 U.S. 435 (1932) there have been discussions on how it should be applied. There are two schools of thought for the application of the entrapment defense. One is the objective test and the other is the subjective test for entrapment. The subjective test focuses on the defendant's predisposition while the objective test focuses on the activity of the government. The federal courts and thirty eight states use the subjective test for entrapment. Florida is one of the states that use the subjective test for entrapment. The majority of this study is focused on the history and application of the entrapment defense, with a focus on the state of Florida. The study will aim to examine the ramifications of applying a subjective test for entrapment. Furthermore, this study will shed some light on problems with the way the entrapment defense is applied in Florida. This study has led to new ideas about how and why a new test for entrapment should be embraced by the courts in Florida.

No description available.

No description available.

Human trafficking has turned into a global criminal industry that makes 32 billion dollars annually and enslaves about 800,000 individuals every year. Eighty per cent of those 800,000 are women or girls, and 50 per cent are minors. Individuals can be subjected to trafficking in many ways; however, the most common form of human trafficking is sex trafficking which involves sexual exploitation. Escaping sex trafficking is not easily done, and in this paper, one will get a better understanding of why it is hard. Knowledge of how girls get entrapped and enmeshed will be shown, but the most important part is showing how hard it can be to escape or what is stopping the victim; it will also show how victims escaped and what happened after they did. This paper looks further into escapes mechanisms from sex trafficking by analysing the stories of four victims of sex trafficking. Joan Reid’s research on Entrapment and Enmeshment (2014) will be used as a theory and the basis for how it is hard for victims to escape and then how they do escape. Research questions for this paper are; Which forms of entrapment did the victims in this study experience? Which forms of enmeshment did the victims in this study experience? How did the victims escape from sex trafficking, and which patterns, if any, are observable in terms of escape strategies? The stories of four victims describe how they were entrapped and enmeshed and how they later escaped. With their stories, this study wanted to investigate if they all could be placed into Reid's categories and if those categories were, therefore, all-inclusive. Most of the stories fit into some category; however, there was always at least one story that did not fit in both entrapment and enmeshment. This shows that the subject should be studied further in order to gain more information.

Understanding volatile evolution associated with active volcanic magmatic systems is of paramount importance because volatiles control and determine the magnitude of an eruption owing to the large change in molar volume that volatile species show depending on their physical state (volatiles dissolved in silicate melts vs. volatiles exsolved as vapor). For active volcanic systems studying the volatile evolution can help to assess the potential hazard associated to a certain locality. Also, volatile evolution in magmatic system controls the formation of certain ore deposits. Despite the importance of understanding volatile evolution of magmatic systems, concentrations of volatiles of evolving magmas are not easily available especially for magmas originated in the deep crust. Fortunately, sample of melts can be entrapped as melt inclusion (MI) into growing igneous minerals in crystalizing magma chamber. After the entrapment, the crystal works as an insulating capsule from the external magmatic environment. Researchers have started to use MI because they provide some advantages in respect to the classical whole rock approach to petrological studies. One of the most important advantages is that MI often represent sample of a deep and non-degassed melt (glass) available at Earth's surface. In fact, with the exception of deep ocean basalts, igneous whole rocks found at the Earth's surface are degassed magmas. This dissertation is a compilation of four publications produced during six years of research and is addressed to give a contribution in understanding the volatile evolution in magmatic systems and also to improve the present understanding of information that can be obtained using the melt inclusions technique. In the first chapter, I present an alternative interpretation of H₂O-CO₂ trends obtained from MI. In this study, we demonstrate that these trends can be due to post entrapment crystallization on the wall of the MI and not to magma ascent. This alternative view is more realistic especially for cases where in the same phenocrysts MI show strongly different CO₂ concentrations. In the second chapter, I present a study to test for the MI reliability in recording volatile concentrations. We used the approach of the melt inclusion assemblage (MIA) that consists of analyzing groups of MI presumably entrapped at the same time and, thus, at same chemical and physical conditions. The results show that most of the MIA studied show consistent volatile concentrations corroborating the reliability of the MI technique. CO₂ shows the highest degrees of variability and we have assessed this behavior mostly to C-contamination in the surface of the sample. The third chapter is a study case (the Solchiaro eruption in Southern Italy) that shows the potential uses of MI to understanding the volatile evolution. I present a model showing the dynamic of the magma based on MI. This study also discusses the origin of anomalous MI and which MI provide the best information. The final chapter is dedicated to test the applicability of the new Linkam TS1400XY heating stage. I was able to show how this new microthermometric tool is capable of homogenizing MI at high temperature and to quench MI to a homogeneous glass state. / Ph. D.

Global civilisation, and therefore population health, is threatened by excessive inequality, weapons of mass destruction, inadequate economic and political theory and adverse global environmental change. The unequal distribution of global foreign exchange adjusted income is both a cause and a reflection of global social characteristics responsible for many aspects of these inter-related crises. ¶ The global distribution of foreign exchange adjusted income for the period 1964-1999 is examined. Using data for more than 99% of the global population, a substantial divergence in its distribution is found. The global Gini co-efficient, adjusted for national income inequality, increased from an already high value of 71% in 1964 to peak at more than 80% in 1995, before falling, very slightly, to 79% in 1999. The global distribution of purchasing parity power income is also examined, for a similar period. Though also found to be extremely unequal, its trend has not been to increased inequality. Implications of the differences between these two trends are discussed. ¶ A weighted time series index of global environmental change (IGEC) for the period 1960-1997 was also calculated. This uses nine categories of global time series environmental data, each scaled so that 100% represents the level of each category in nature prior to anthropogenic change; zero represents decline to a critical point. This index fell from 82% in 1960 to 55% in 1997, and will further decline during this century. ¶ Using evidence from several disciplines, it is argued that the decline in the IGEC correlates with major macro-environmental changes, which, combined with flawed social responses to scarcity and its perception, place at risk the ability of civilisation to function. This could occur because of the interaction of conflict, economically disastrous extreme climatic events, deterioration of other ecosystem services, regional food and water insecurity, and currently unforeseen events. Uncertainty regarding both a safe rate of decline and the tolerable nadir of the IGEC is substantial. ¶ Substantial reduction in the inequality of foreign exchange adjusted income is vital to enhance the development of policies able to reverse the decline in the environmental goods which underpin civilisation, and to promote the co-operation needed to maximise the chance that civilisation will survive.

Plantar heel pain is commonly encountered by clinicians. Various conditions, such as plantar fasciopathy, myofascial syndrome and entrapment of the tibial, plantar and calcaneal nerves at the tarsal tunnel can cause plantar heel pain. This diversity in aetiology makes the diagnosis and treatment challenging. There are limited studies on pain mechanisms in patients with planter heel pain. There is no gold criterion standard for the diagnosis. Although various interventions have been reported, no specific treatment approach has yet been identified as being most effective. The first aim of this thesis was to critically appraise the literature on plantar heel pain of neural origin. Various databases were searched for peer-reviewed articles that predominantly focused on neurogenic plantar heel pain or that discussed relevant biomechanics of the tibial, plantar and calcaneal nerves. This review revealed inconsistency in the literature regarding the diagnosis and treatment of neurogenic plantar heel pain. There also was a lack of evidence for treatment approaches although the majority of patients with plantar heel pain are reported to improve with conservative treatment. The second aim of this thesis was to examine the biomechanical effects of clinical tests and combination of movements on various structures associated with plantar heel pain. This aim was achieved through cadaver studies (Study 1–3), in which strain in the plantar fascia and the nerves of the lower limb, and excursion of the nerves were measured during various movements and positions of the lower limb. Study 1 examined the Dorsiflexion-eversion test used to diagnose tarsal tunnel syndrome (TTS) and the Windlass test for plantar fasciopathy given the similarity between both tests. Both the Dorsiflexion-eversion and Windlass tests significantly increased strain in the structures that are commonly associated with plantar heel pain (the tibial and plantar nerves and plantar fascia). This suggests that the usefulness of the Dorsiflexion-eversion and Windlass tests in the differential diagnosis of plantar heel pain might be limited. Study 2 investigated the influence of different positions in adjacent joints on nerve biomechanics during ankle and toe movement. Increased strain in the tibial nerve at the ankle and plantar nerves associated with ankle and toe movement was significantly higher when the nervous system was pre-tensioned at a more proximal joint. Strain was even higher when the nerve bed was pre-tensioned at two joints. Study 3 examined a modified straight leg raising (SLR) test in which ankle dorsiflexion is performed before hip flexion. This test has been suggested to diagnose distal neuropathies such as TTS. During the modified SLR, the excursion and strain in the sciatic nerve associated with hip flexion were transmitted distally along the nerve from the hip to the foot. As a result, the strain in the nerves around the foot and ankle increased significantly during hip flexion. This movement did not affect plantar fascia strain. Consequently, the modified SLR may be a useful test to differentially diagnose plantar heel pain. This test warrants future research to evaluate its clinical use in patients with neurogenic plantar heel pain. The third aim of this thesis was to determine the reliability of high-resolution ultrasound for measuring the cross-sectional area of the tibial nerve at the tarsal tunnel and to compare the tibial nerve size between people with and without plantar heel pain. Study 4 investigated intra and intertester reliability in 10 participants without plantar heel pain by calculating intraclass correlation coefficients, measurement error and smallest detectable difference (SDD). Intra and intertester reliability were excellent, with very small measurement error and SDD. Tibial nerve enlargement in an individual patient by as little as 1.8 mm2 can be detected reliably with high-resolution ultrasound. The use of average value of three scans is recommended to compare between the involved and uninvolved side. Differences in the nerve size between 26 patients with plantar heel pain and 20 control participants were also analysed. There was no significant difference in tibial nerve size between both groups. Future research is needed to investigate the tibial nerve size in patients with proven TTS using ultrasonography. The fourth aim of this thesis was to investigate the characteristics of plantar heel pain through Study 5 for the same group of patients and control participants as in Study 4. Several self-report measures on pain and quality of life were used. Clinical tests and quantitative sensory tests (QST) were performed at local and remote sites on the involved and uninvolved side in the patients and on one side in the control participants. In the patients, mechanical hyperalgesia was the main finding as demonstrated by changes in palpation and pressure pain threshold. Other findings were changes in current thresholds, vibration threshold and thermal perception thresholds. These results suggest the existence of sensory changes that likely indicates change in peripheral and central pain processing. It is recommended to utilise a multidimensional pain assessment for patients with plantar heel pain. The findings in this thesis are important for the diagnosis and treatment of plantar heel pain. For future research, the results suggest to use fresh cadavers when investigating biomechanics of the clinical tests and nerve gliding exercises that are used for patients with plantar heel pain. It is also suggested to evaluate the cross-sectional area of the tibial nerve at the tarsal tunnel, the QSTs and all other diagnostic measurements in this thesis in patients with neurogenic plantar heel pain or patients with TTS.

Water infiltration into the unsaturated zone especially in a shallow water table environment is affected by air compression ahead of the wetting front and air counterflow. Neglecting air compression in infiltration modeling can overestimate infiltration and infiltration rates, whereas not accounting for air counterflow can underestimate infiltration and infiltration rates due to unrealistic buildup of air pressure resistance ahead of the wetting front. A method, derived on the basis of the Green and Ampt (1911) infiltration model, is introduced to simulate air compression and air counterflow during infiltration into a shallow water table. The method retains the simplicity of the Green and Ampt (1911) model but adds the air pressure resistance term ahead of the wetting front. Infiltration equations are derived on the basis of the Green and Ampt (1911) and Sabeh’s (2004) infiltration model which accounts for air compression and air counterflow. The difference between this method and Sabeh’s (2004) model is that air counterflow, air compression, and infiltration are decoupled and updated with each wetting front increment whereas Sabeh’s (2004) method uses time step as a decoupling mechanism. Air compression ahead of the wetting front is predicted using the perfect gas law. Laboratory experiments showed that the introduced method is reasonably accurate when modeling cumulative infiltration values. Results of laboratory experiments were compared to results of the modeled infiltration methods: original Green and Ampt (1911) model and Green and Ampt with air compression and counterflow. The advantage of this new method is its simplicity. The new method uses parameters that are generally needed for modeling infiltration with the Green and Ampt (1911) approach. Disadvantages of the model are assumptions of the uniform water content and the uniform pressure. Another shortcoming of the model is that it does not account for air compression and air counterflow prior to ponding. Laboratory experiments described in this work and a proposed model can be further used for modeling and studying infiltration with air effects. In addition, this work can be of use to someone studying irrigation techniques of rice or other crops.

Numerical simulations indicate that hydrocarbon migration and entrapment in stacked fault-bounded reservoirs are mainly affected by the following factors: charge time, faults, pressure and geological structures. The charge time for commercial hydrocarbon accumulation is much longer in oil-water systems than in oil-gas-water systems. Faults are classified into charging faults and 'back doors' faults other than charging faults in stacked fault-bounded reservoirs. The lower the displacement pressure of a fault, the higher its updip oil transportation ability. The downdip oil transportation ability of a fault is usually low and cannot cause commercial downdip oil accumulation. Back doors affect both hydrocarbon percent charge and hydrocarbon migration pathways. Updip back doors improve updip oil charge. The lower the displacement pressure of an updip back door, the more efficient the updip oil charge before 3,000 years. Back doors whose displacement pressure is equal to or higher than 28.76 psi are effective in sealing faults in oil-water systems. On the contrary, only sealing faults result in commercial gas accumulations in stacked fault-compartmentalized reservoirs. Otherwise gas is found over oil. Downdip back doors generally have few effects on downdip hydrocarbon charge. Geopressure enhances the updip oil transportation of a fault and improves the positive effects of updip back doors during updip oil charge. Geopressure and updip back doors result in more efficient updip oil charge. A physical barrier is not necessarily a barrier to oil migration with the aid of geopressure and updip back doors. The chance for hydrocarbon charge into reservoirs along growth faults is not equal. Any one of the above controlling factors can change the patterns of hydrocarbon charge and distribution in such complex geological structures. Generally, lower reservoirs and updip reservoirs are favored. Reservoirs along low-permeability charging faults may be bypassed. Gas can only charge the updip reservoirs. Both updip and downdip back doors can facilitate oil penetrating a barrier fault to charge reservoirs offset by the barrier fault. Interreservoir migration among stacked fault-compartmentalized reservoirs is an important mechanism for hydrocarbon accumulation and trap identification. The interreservoir migration is a very slow process, even though the displacement pressures of bounding faults may be very low.