Posttraumatic stress disorder (PTSD) has been linked to heightened engagement in risky sexual behavior (RSB) across diverse samples, and impulsivity has been postulated as a potential linkage (Weiss et al., 2012). Limited information has been published on the role that impulsivity can play in strengthening the relationship between PTSD and RSB in college students. The current study examined the moderating role of impulsivity dispositions: negative/positive urgency, (lack of) perseverance, sensation seeking, and (lack of) premeditation on the association between PTSD symptoms and past-year RSB among a sample of 221 undergraduate students (77.4% female) with at least one DSM-5 defined traumatic event. Negative binomial regression models were conducted to explore each impulsivity disposition’s moderating effect on the relationship between PTSD symptoms and RSB. PTSD symptoms and positive urgency, (lack of) perseverance, and sensation seeking were independently associated with RSB. Significant interactions were found between negative urgency and PTSD symptoms, and (lack of) premeditation and PTSD symptoms, such that PTSD symptoms were more strongly linked to RSB among individuals high in these impulsivity dispositions. The present study expands on the limited literature on the role specific impulsivity dispositions can have in the relationship between PTSD and RSB in trauma-exposed college students.

<p><em>Background and problem:</em> In March 15, 2009, the third piece of money laundering directive was introduced which mean a more strict legislation in control against money laundering and terrorist financing. The directive is based on a risk based point of view which means that the resources should be used where the needs are. The responsibility for financial companies and other parts are bigger then before which means that they have to get a more extensive customer knowledge etc. It has however been appeared that the directive in some cases lack of precision and clarity which creates an insecurity among those who are included by the law. </p><p><em>Purpose:</em> The purpose of this paper is to examine which consequences the third piece of money laundering will bring on the banks customer relationships and how the employees work assignments at the banks will be affected. The authors also want to examine how the extension of money laundering will be affected and clarify if there is any indistinctness about how the application looks around the banks.</p><p><em>Method:</em> The authors choose to use a qualitative method and the empiric material has been collected through personal meetings, telephone interviews and through e-mail answers.</p><p><em> </em></p><p><em>Conclusion:</em> The majority sees the third piece of money laundering directive as something positive which will protect both the banks and their customers. Although it has been showed that the banks think that some parts of the directive is both unclear and meaningless since they don’t get the tools which are required to complete the work. The customers of the banks will be exposed for more and personal questions then before which has led to that the customers in some cases feel insulted. Since also the employees of the banks will be affected of the new routines and have to deal with irritated customers, it is important that they will experience good internal education. The authors have discovered some lack in these educations since the employees aren’t aware of that they personally can be judged to fines if they don’t live up to the legislation.</p><p> </p><p><em>Future studies:</em><em> </em>A proposal for future studies is to after a time examine if the directive has got the effect that the legislation aims to, videlicet examine<strong> </strong>if the extent of money laundering has been reduced. <em></em></p><p><em> </em></p><p><em>Keyword:</em> Risk based point of view, the third piece of money laundering directive, agency theory, and politically exposed persons<em></em></p><p><em> </em></p>

Mycobacterium avium subsp. hominissuis (MAH) is a pervasive environmental bacterium that can cause opportunistic infections in humans. Among the most robust and hardy members of the Mycobacterium genus, M. avium can persist and thrive in a range of challenging environments, including many which place it in direct contact with humans. Surface-exposed proteins are central to the bacterial processes involved in both environmental persistence and pathogenesis. These proteins also play a critical role in how the immune system of the host recognizes and responds to pathogens. Mycobacteria have evolved a specialized mechanism for protein export, a Type VII Secretion System (T7SS), in order to transport their proteins through their thick and impermeable cell envelope. This system is responsible for the export of several classes of proteins, many of which play an integral role in virulence. A central focus of this dissertation is the characterization of a conserved element of the T7SSs in pathogenic mycobacteria, a PPE family protein, whose deletion attenuates virulence in M. avium. Specifically, we examined the localization of this PPE protein (MAV_2928) within the bacterium, screened potential protein-protein interactions with other conserved elements in the adjacent T7SS loci and analyzed the transcriptional regulation of the gene in response to environmental changes. Seeking to more thoroughly characterize the surface-exposed proteome of M. avium, particularly in the context of early infection, we then developed a method, based on selective biotinylation and affinity purification, to profile the of surface-exposed proteome of the bacterium. We employed this method to analyze the surface-exposed proteomes of M. avium 109 that had been exposed to macrophages to those of M. avium 109 that had been cultured in media. This comparison detected several proteins whose presence at the bacterial surface appeared to be dependent on particular growth conditions. Lastly, in order to establish a more efficient method to isolate biotinylated surface proteins from complex mixtures, we developed a testing paradigm to identify modifications to the original method that might improve our coverage of identified proteins. Through this process, we developed a more robust methodology that yielded improved coverage and depth. We then utilized this technology to profile the surface-exposed proteome of another clinical isolate of M. avium subsp. hominissuis, M. avium 104. Beyond improving our understanding of the basic biology of M. avium, this new data provides independent evidence that PPE family proteins are indeed exported to the surface of M. avium, where they remain associated with the bacterial cell envelope. In total, this analysis represents the most comprehensive profile of the surface-exposed proteins of M. avium generated to date. / Graduation date: 2012

Background and problem: In March 15, 2009, the third piece of money laundering directive was introduced which mean a more strict legislation in control against money laundering and terrorist financing. The directive is based on a risk based point of view which means that the resources should be used where the needs are. The responsibility for financial companies and other parts are bigger then before which means that they have to get a more extensive customer knowledge etc. It has however been appeared that the directive in some cases lack of precision and clarity which creates an insecurity among those who are included by the law.  Purpose: The purpose of this paper is to examine which consequences the third piece of money laundering will bring on the banks customer relationships and how the employees work assignments at the banks will be affected. The authors also want to examine how the extension of money laundering will be affected and clarify if there is any indistinctness about how the application looks around the banks. Method: The authors choose to use a qualitative method and the empiric material has been collected through personal meetings, telephone interviews and through e-mail answers.   Conclusion: The majority sees the third piece of money laundering directive as something positive which will protect both the banks and their customers. Although it has been showed that the banks think that some parts of the directive is both unclear and meaningless since they don’t get the tools which are required to complete the work. The customers of the banks will be exposed for more and personal questions then before which has led to that the customers in some cases feel insulted. Since also the employees of the banks will be affected of the new routines and have to deal with irritated customers, it is important that they will experience good internal education. The authors have discovered some lack in these educations since the employees aren’t aware of that they personally can be judged to fines if they don’t live up to the legislation.   Future studies: A proposal for future studies is to after a time examine if the directive has got the effect that the legislation aims to, videlicet examine if the extent of money laundering has been reduced.   Keyword: Risk based point of view, the third piece of money laundering directive, agency theory, and politically exposed persons

In the essay, the balance between being an educator and a mother of a child with autism is explored in respect to how the roles might influence the actions of a pre-school teacher. Writing is used as a method of reflection, it gives the teacher a chance to take a step back and view her practical experiences from a novel perspective; conclusions are recounted throughout the teachers narrative. The essay is based on the following questions; which problems might arise in the meeting with parents such as Adams where there is a suspicion of autism for the child? Which advantages/disadvantages might the teacher’s personal experience of autism have for the role as educator? How would the dual roles as educator and mother of an autistic child best be balanced in the future? The essay highlights special educational and parent perspectives on the matter of autism. Professor of pedagogy Claes Nilholm, specializes in special needs education. Nilholm discusses the existence of compensatory and critical perspectives on autism in the classroom, which are presented in the essay as a problematic dichotomy which the teacher must navigate. Based on the work performed by psychologist Lennart Lindqvist the teacher presents her own experiences of being a mother of a child with autism and how it affects her role as educator. From the reflective process the teacher concluded that personal experience from children with autism might be of advantage in the educational environment but has distinct disadvantages when communicating with parents. Aristoteles three forms of knowledge episteme, techne and fronesis were used to further highlight the difficulties when dealing with parents of children with suspected autism. Especially the concept of fronesis was discussed in relation to the teacher’s narrative as an influencing factor. Fronesis, the wisdom to act correctly in a given situation, was concluded be of advantage in the role as educator. When meeting parents of children with suspected autism the teachers unprocessed feeling on the matter may become a disadvantage. The aim of the essay was to deepen the knowledge of the teachers own experiences in order to better utilize them in the role as an educator.

Understanding natural mechanisms of protection against HIV infection and disease progression are key priorities for informing vaccine and microbicide design. The research presented in this thesis aimed to characterize mechanisms of defence in HIV-exposed seronegative (HESN) individuals, who naturally resist infection by HIV, and HIV-controllers, who are HIV-infected, but suppress viral replication in the absence of treatment. Previous studies have linked resistance to HIV infection with low basal levels of gene transcription and reduced production of inflammatory mediators, suggesting an overall state of immune quiescence in HESN. Immune quiescence may also be protective in HIV-infected individuals, as immune activation drives disease progression. The central hypothesis of this thesis is that immune quiescence protects against HIV infection and disease progression by limiting the pool of activated target CD4+ T cells susceptible to HIV infection. This hypothesis was addressed by evaluating immune function in HESN from the Pumwani commercial sex worker cohort and HIV-controllers from the Manitoba elite controller cohort. In HESN, immune quiescence was marked by low levels of circulating activated T cells and low levels of the proinflammatory mediators IL-1α and IL-8 in the cervical mucosa. Regulatory T cells (Tregs), which suppress T cell activation, were elevated in HESN, and may represent a driver of immune quiescence. Low T cell activation and elevated Tregs were associated with reduced cellular susceptibility to infection in vitro. These data suggest that immune quiescence protects against infection by limiting the activated target CD4+ T cell pool, in support of the central hypothesis. HIV-controllers expressed low levels of the proinflammatory chemokines IP-10 and MCP-1 and low frequencies of activated T cells. These data demonstrate that immune quiescence is not only protective prior to exposure, but is also beneficial following infection. HIV-controllers also had elevated MIP-1α, reduced TGFβ and HIV-specific T cell proliferation responses, which contribute to protection by mechanisms other than immune quiescence. Taken together, these data support a role for immune quiescence in protection from HIV infection and disease progression. Mechanisms of reducing inflammation and target cell activation should be considered during future HIV vaccine and microbicide development.

With sufficient time, a high density polyethylene geomembrane will degrade and lose its engineering properties until ruptures signal the end of its service-life. This thesis examines the longevity of nine different geomembranes; five of them were of different thickness manufactured from the same resin. The degradation of properties and time to failure are investigated for geomembranes: in immersion tests; as a part of a landfill composite liner; and, exposed to the elements. The different thermal and stress histories associated with manufacturing geomembranes of different thickness are shown to affect their morphological structure; consequently, their stress crack resistance. When immersed in synthetic leachate, it was found that: (a) thicker geomembranes have a longer antioxidants depletion time but the effect of thickness decreases with temperature and is less than expected; (b) inferences of geomembrane’s longevity based on its initial properties may be misleading because a geomembrane may chemically degrade (as manifested by the change in melt index) despite the presence of a significant amount of stabilizers (as manifested by the measured high pressure oxidative induction time); and, (c) stress crack resistance may change before antioxidant depletion or chemical degradation takes place, likely, due to changes in geomembrane morphological structure with the maximum decrease being observed at 55oC. Reductions also were measured for geomembrane immersed in air and water at 55oC. The geomembrane aged in a simulated landfill liner at 85oC is shown to have service-life as little as three years with 30,000 to >2.0 million ruptures/hectare at failure. For exposed geomembranes in Alumbrera (Argentina), samples were exhumed from two mine facilities after ~16 years of exposure. The antioxidants in exposed samples depleted to residual and the stress crack resistance had dropped to as low as 70 hours. Samples were exhumed from a different exposed geomembrane in a test site in Godfrey (Canada) after six years of exposure. The antioxidants were partially depleted, with depletion to residual projected to take at least 20 years; however, despite no evidence of chemical ageing, the stress crack resistance had decreased from 330 to 190 hours, likely due to changes in the morphological structure of the geomembrane. / Thesis (Ph.D, Civil Engineering) -- Queen's University, 2014-02-28 04:59:20.834

Understanding natural mechanisms of protection against HIV infection and disease progression are key priorities for informing vaccine and microbicide design. The research presented in this thesis aimed to characterize mechanisms of defence in HIV-exposed seronegative (HESN) individuals, who naturally resist infection by HIV, and HIV-controllers, who are HIV-infected, but suppress viral replication in the absence of treatment. Previous studies have linked resistance to HIV infection with low basal levels of gene transcription and reduced production of inflammatory mediators, suggesting an overall state of immune quiescence in HESN. Immune quiescence may also be protective in HIV-infected individuals, as immune activation drives disease progression. The central hypothesis of this thesis is that immune quiescence protects against HIV infection and disease progression by limiting the pool of activated target CD4+ T cells susceptible to HIV infection. This hypothesis was addressed by evaluating immune function in HESN from the Pumwani commercial sex worker cohort and HIV-controllers from the Manitoba elite controller cohort. In HESN, immune quiescence was marked by low levels of circulating activated T cells and low levels of the proinflammatory mediators IL-1α and IL-8 in the cervical mucosa. Regulatory T cells (Tregs), which suppress T cell activation, were elevated in HESN, and may represent a driver of immune quiescence. Low T cell activation and elevated Tregs were associated with reduced cellular susceptibility to infection in vitro. These data suggest that immune quiescence protects against infection by limiting the activated target CD4+ T cell pool, in support of the central hypothesis. HIV-controllers expressed low levels of the proinflammatory chemokines IP-10 and MCP-1 and low frequencies of activated T cells. These data demonstrate that immune quiescence is not only protective prior to exposure, but is also beneficial following infection. HIV-controllers also had elevated MIP-1α, reduced TGFβ and HIV-specific T cell proliferation responses, which contribute to protection by mechanisms other than immune quiescence. Taken together, these data support a role for immune quiescence in protection from HIV infection and disease progression. Mechanisms of reducing inflammation and target cell activation should be considered during future HIV vaccine and microbicide development.

Improving the performance of the wireless multihop ad hoc networks faces several challenges. In omni-directional antenna based solutions, the use of the RTS/CTS mechanism does not completely eliminate the hidden-terminal and exposed-terminal problems. Deafness is an additional challenge to the directional antenna based solutions. This dissertation, first develops analytical models for quantifying the throughput and delay in wireless multihop ad hoc networks. The models consider the impact of hidden terminals using the realistic signal to interference and noise ratio model and consider random node distribution. The proposed analysis is applicable to many wireless MAC protocols and applications. The analytical results reveal several important issues. The first issue is quantifying the impact of adjusting the transmission range on the throughput and delay in wireless multihop ad hoc networks. The other issue is the hidden terminal region is closely related to the distance between the transmitter and the receiver. Thus, it is possible to adjust the transmission range to optimize the whole network performance. These results provide important guidelines for network planning and protocol optimization in wireless multihop ad hoc networks. Second, it proposes a new Enhanced Busy-tone Multiple Access (EBTMA) medium access control (MAC) protocol for minimizing the negative impact of both the hidden-terminal and the exposed-terminal problems. The new protocol can also enhance the reliability of packet broadcasts and multicasts which are important for many network control functions such as routing. Different from other busy-tone assisted MAC protocols, the protocol uses a non-interfering busy-tone signal in a short period of time, in order to notify all hidden terminals without blocking a large number of nodes for a long time. In addition, the proposed EBTMA protocol can co-exist with the existing 802.11 MAC protocol, so it can be incrementally deployed. Third, it investigates how to support the directional antennas in ad hoc multihop networks for achieving higher spatial multiplexing gain and thus higher network throughput. A new MAC protocol called Dual Sensing Directional MAC (DSDMAC) protocol for wireless ad hoc networks with directional antennas is proposed. The proposed protocol differs from the existing protocols by relying on a dual sensing strategy to identify deafness, resolve the hidden-terminal problem and to avoid unnecessary blocking. Finally, this dissertation provides important results that help for network planning and protocol optimization in wireless multihop ad hoc networks in quantifying the impact of transmission range on the throughput and the delay. The accuracy of these results has been verified with extensive discrete event simulations. / Graduate

Hepatitis C virus (HCV) is a leading cause of chronic liver disease. In the developed world, injection drug use (IDU) through sharing of infected needles and other paraphernalia remains the principal risk factor for HCV transmission. Effective but expensive treatment is now possible but there remains a pressing need for a vaccine. A proportion of people who inject drugs (PWIDs) remain uninfected despite HCV exposure from a long history of sharing needles and other paraphernalia. These cases are termed exposed but uninfected (EU) and test negative for both HCV antibodies and RNA and exhibit a phenotype of resistance to HCV infection. Improved understanding of the mechanisms that confer resistance in the EUs has the potential to aid development of an effective vaccine and novel therapeutic strategies. This thesis reports on the findings from 3 different strategies to identify characteristics of HCV resistance. I used urinary metabolomics, serum lipidomics and the study of adaptive and innate immune responses. Each of these methods has demonstrated clear differences between EU cases and healthy controls and/or spontaneous resolvers of HCV infection. Urinary metabolomics suggest a potential role of the gut microbiome, the serum lipidomics showed marked differences in lipid profiles in EU cases pointing towards a perturbed lipid/virus interaction, and the immune studies confirmed previous work identifying low level T cell responses in many EU cases but has also identified a marked upregulation of interferon alpha production to low dose viral RNA in EU cases utilising ELISA assay. In conclusion, this thesis reports data that identifies a number of new findings that provide insight into mechanisms of resistance to HCV infection. My findings suggest that the complex interplay between the virus and lipids together with an upregulated innate immune response may together help determine the outcome following HCV exposure. In summary, studies performed in this thesis have demonstrated that there are different pathways that define the EU phenotype. Despite being a heterogenous subgroup of PWIDs, the EUs are clearly distinct from a healthy control population.