Many insects form close relationships with microbial symbionts. Insect symbionts can provide novel phenotypes to their hosts, including influencing dietary breadth. In the polyphagous cowpea aphid, Aphis craccivora, the facultative symbiont Arsenophonus improves aphid performance on one host plant (locust), but decreases performance on other plants. The goal of my thesis was to investigate the mechanism by which Arsenophonus facilitates use of locust. First, I assembled an Aphis craccivora-Arsenophonus-Buchnera reference transcriptome to conduct RNAseq analysis, comparing gene expression in aphids feeding on locust and fava, with and without Arsenophonus infection. Overall, few transcripts were differentially expressed. However, genes that were differentially expressed mapped to a variety of processes, including metabolism of glucose, cytoskeleton regulation, cold and drought regulation, and B-vitamin synthesis. These results imply that Arsenophonus is producing B-vitamins, which might be deficient in locust. In a second set of experiments, I used qPCR to test whether symbiont function across host plants might be mediated by bacterial titer. I measured relative Arsenophonus abundance across plants, and found Arsenophonus titer was variable, but generally greater on locust than fava. In summary, my results suggest that Arsenophonus synthesis of B-vitamins should be further investigated and may be mediated by bacterial titer.

Effects of exercise, social facilitation, and delayed conditioning after vi exercise on the learning behavior of 5 to 6-inch rainbow trout, Salmo gairdneri, was measured in a conditioned avoidance response apparatus. The conditioning schedule contained an intertrial interval between alternate presentations of conditioned (light) and unconditioned (shock) stimuli. This randomization eliminated learning losses found in a previous study. Mean percentage avoidance, the measurement of learning, did not decrease significantly during conditioning trials. Exercised fish learned avoidance better than did non-exercised fish. Mean percentage avoidance for fish exercised at 0.5 ft/sec was 66.3; at 1.0 ft/ sec, 1.5 ft/sec, and no-exercise, mean percentage avoidance was 68.2, 68.9 and 65.0, respectively. Social facilitation affected learning in the one, two, and three fish per cell tests, where mean percentage avoidance was 55.5, 68.9, and 81.0 percent, respectively. A delay of 24 hours between exercise and conditioning resulted in decreased learning levels. Mean percentage avoidance was 60.3, 63.5, 67.7, and 53.7 for the 1-, 2-, 2-, and 24-hour delay tests, respectively; however, mean percentage avoidance for the last 60 trials of each test indicated the 1-, 2-, and 4-hour delay tests were all similar, over 70 percent, while mean percentage avoidance for the 24-hour delay test was only 57.8 percent.

[Truncated abstract] In order to assess the sustainability of mixed plantings on saline land, this thesis examined the importance of leaf-litter trapping and microsites on recruitment in a salt affected alley-belted (tree/shrub) agricultural system in Western Australia. Located in the low rainfall region (MAR <330 mm) of the wheatbelt, the 60 ha site consists of concentric rows of Eucalyptus sargentii trees with mounded (6 - 11 cm high) 10 -15 m inter-rows of Atriplex spp. Sustainability of this system and fulfilment of productive and ameliorative functions is dependant on successful recruitment (perennials). Although the present study site was conducted on farmland in a Mediterranean-type climate, low annual rainfall and spatial arrangement of perennial shrubs and trees, allow useful comparisons to be made with naturally occurring banded semi-arid systems and vice-versa. Of key interest were leaf-litter redistribution and trapping by tree and shrub rows and whether litter-cover/microsites facilitated/interfered with seedling recruitment (establishment, growth and survival). Litter from the tree row, redistributed by prevailing winds and rain, accumulated adjacent to saltbush seeding mounds, creating a mosaic of bare and littered areas across the site (total litter 10 t/ha over 22 months). Accumulated litter was hypothesized to differentially influence seasonal soil abiotic parameters (depending on litter-cover density) including; salinity, water availability, infiltration rates, water repellency and temperature. These abiotic conditions were also hypothesized to vary between tree and shrub microsites. Biotically, recruitment at this site was also hypothesized to be determined by interactions (positive and negative) between perennial components and understorey annuals/perennial seedlings. Accumulation of litter and resultant heterogeneity was influenced by shrub morphology, microtopography, wind direction and distance from litter source, with increased litter on the leeward sides of hemispherical Atriplex undulata shrubs and shrubs closest to tree rows. ... The importance of tree/shrub microsites varied seasonally, with no influence in winter due to moderate temperatures and increased water availability. In warmer months saltbush mid-row microsites were most favourable for seedling recruitment due to moderate litter-cover; reducing salinity, temperatures and increasing infiltration; and reduced root-competition/shading by the tree row. Tree microsites also directly inhibited seedling recruitment through increased salinities and water repellency. However, trees also indirectly facilitated recruitment in adjacent areas through provision of leaf-litter. As litter-trapping and recruitment patterns at this site mirror those found in semi-arid natural and artificial systems, the results of this study provide useful insights into creating appropriate mimics of low rainfall natural banded woodland and chenopod shrublands. Saltbush seeding mounds, shrub morphology and litter were key components for litter trapping and recruitment heterogeneity at this site. In this tree/shrub alley planting, where litter quantities directly influence vegetation cover densities, future saline plantings need to consider appropriate tree/shrub row spacings and orientation for efficient resource (seeds, litter and water) capture.

La réaction acrosomique de Mammifère nécessite l'ouverture successive de trois canaux calciques : un canal calcique activé par de faibles dépolarisations (LVA), le récepteur à l'IP3 et un canal activé par la vidange des stocks (SOC) TRPC2. Nos données montrent une intéressante interaction fonctionnelle entre le canal LVA et les protéines dont l'activité est liée au niveau de remplissage du réticulum (vraisemblablement les canaux TRPCs et l'IP3R). Toute la signalisation calcique de la RA est sous le contrôle du canal LVA qui est le premier à s'activer. En utilisant les souris déficientes pour Cav3.1, Cav3.2, nous avons montré que la sous-unité α1H est la sous-unité majoritaire dans les cellules spermatogéniques sauvages. Nos données fournissent de nouvelles hypothèses concernant l'activation de TRPC2 : celle-ci pourrait être due à des modifications de son interaction avec la junctate et l'IP3R, induite par la vidange des stocks.

<p>The increase of women’s labor force participation, the diversity of family constellations, many men’s increased participation in childcare, and the development of flexible working arrangements have stimulated research on the relationship between work and non-work. In this thesis, the interaction between work and non-work is examined in the case of home-based telework, an example of flexible working arrangement. Because of the disappearance or blurring of the traditional boundaries in time and space, the consequences of these working conditions for individuals need to be analyzed. The overall aim of this thesis is to study the interaction between work and non-work in home-based telework and to analyze the following issues:</p><p>- conflict versus facilitation as related to the segmentation and integration of work/non-work domains</p><p>- the nature and role of boundaries</p><p>- the physical and mental aspects of work activities within the domains</p><p>The thesis is based on quantitative as well as qualitative data. In general, the results show that the domains of work and non-work do interact with each other in various ways and dimensions. The employees reported some degree of overlap spatially, temporally, and mentally, and limited overlap was preferred by the majority. The results show the importance of boundaries, as they are needed in order to bring structure and legitimacy to the work (carried out in the home/private domain) and to detach from work. The teleworking women reported less effective restoration than the non-teleworking women, while the teleworking men reported slightly more effective restoration than the non-teleworking men. Generally, the results showed that work tended to intrude more on family and non-work than vice versa. Individuals who experienced conflict between the domains of work and non-work also reported a lower degree of life satisfaction. Significant associations were found between facilitation (positive spillover) and life satisfaction and recovery.</p>

The increase of women’s labor force participation, the diversity of family constellations, many men’s increased participation in childcare, and the development of flexible working arrangements have stimulated research on the relationship between work and non-work. In this thesis, the interaction between work and non-work is examined in the case of home-based telework, an example of flexible working arrangement. Because of the disappearance or blurring of the traditional boundaries in time and space, the consequences of these working conditions for individuals need to be analyzed. The overall aim of this thesis is to study the interaction between work and non-work in home-based telework and to analyze the following issues: - conflict versus facilitation as related to the segmentation and integration of work/non-work domains - the nature and role of boundaries - the physical and mental aspects of work activities within the domains The thesis is based on quantitative as well as qualitative data. In general, the results show that the domains of work and non-work do interact with each other in various ways and dimensions. The employees reported some degree of overlap spatially, temporally, and mentally, and limited overlap was preferred by the majority. The results show the importance of boundaries, as they are needed in order to bring structure and legitimacy to the work (carried out in the home/private domain) and to detach from work. The teleworking women reported less effective restoration than the non-teleworking women, while the teleworking men reported slightly more effective restoration than the non-teleworking men. Generally, the results showed that work tended to intrude more on family and non-work than vice versa. Individuals who experienced conflict between the domains of work and non-work also reported a lower degree of life satisfaction. Significant associations were found between facilitation (positive spillover) and life satisfaction and recovery.

Nearly two decades of research on translating evidence-based care guidelines into practice has resulted in a considerable body of primary and secondary evidence about guideline implementation strategies and the individual, organizational and environmental challenges associated with closing the evidence to practice gap in primary care. Interventions to improve professional performance are complex and a disentangling of the various independent, intervening and constraining variables is required in order to be able to design and implement interventions that can improve primary care practice performance. The PRECEDE-PROCEED planning model (Green & Kreuter, 1999) provides a step-wise theoretical framework for understanding the complexity of causal relationships among the variables that affect the adoption of evidence-based practice and may assist in the design and implementation of practice-based interventions. Knowledge of an evidence-based practice guideline is important, but a consensus has emerged that having knowledge is rarely sufficient to change practice behaviour. Didactic education or passive dissemination strategies are ineffective, whereas interactive education, reminder systems and multifaceted interventions tailored to the needs of the practice are effective. Outreach or practice facilitation is a proven effective multifaceted approach that involves skilled individuals who enable others, through a range of tailored interventions, to address the challenges in implementing evidence-based care guidelines within the primary care setting. The challenges to implementing evidence-based chronic illness care practice guidelines are thought to be similar to the other contextual, organizational and individual behavioural challenges associated with the uptake of research findings into practice. A multifaceted guideline implementation strategy such as practice facilitation may be well-suited to improving the adoption of these guidelines within rural primary care settings. However, research has not systematically reviewed, through meta-analysis, the published practice facilitation trials to determine overall effects and an implementation research study of practice facilitation that has considered fidelity of implementation within the rural Ontario setting for a complex practice guideline such as chronic illness management has not been done. The systematic review in the thesis incorporated an exploratory meta-analysis of randomized and non-randomized controlled trials of interventions targeted towards implementing evidence-based practice guidelines through practice facilitation, and was conducted to gain an understanding of the overall effect of practice facilitation and the factors that moderate implementation success. The results were the identification of an improvement overtime in the methodological rigour of practice facilitation implementation research based on a critical appraisal of methods, a significant moderate overall effect size of 0.54 (95% CI 0.43 – 0.65) for 19 good quality practice facilitation intervention studies and several significant effect size modifiers; notably, tailoring to the needs of the practice, using multiple intervention components, extending duration, and increasing the intensity of practice facilitation were associated with larger effect sizes. As more practices were assigned to the practice facilitator, the effect diminished. A significant positive association between the number of PRECEDE predisposing, enabling and reinforcing strategies employed by the facilitator and the effect size was detected. The implementation research study utilized mixed methods for data collection as part of an embedded case study of four rural primary care practices to determine the implementation fidelity of the practice facilitation of chronic illness care planning and the factors that impeded and contributed to implementation success. The feasibility of and potential cost savings of practice facilitation via videoconferencing was also implemented for two of the practices. For those practices that successfully implemented care planning, fidelity was achieved for the implementation of care plans. On the other hand, the dosage, duration, component delivery of the practice facilitation intervention was low in comparison to other published studies, and tailoring of the intervention to the practice was inconsistent. Based on the qualitative analysis of physician interviews, the moderating factors for successful implementation were categorized into the broad themes of pessimism and tempered optimism. Pessimistic physicians were unsuccessful at implementation, lacked a willingness to engage and were uncomfortable with the patient-centred approach to chronic illness care. Optimists were positive about the psychosocial, patient-centred assessment aspects of the chronic illness care protocol and provided anecdotes of success in resolving patient problems. However, this was tempered as both pessimists and optimists reflected on the time intensive aspect of the protocol and the unlikelihood of widespread implementation without additional supports. Participating physicians were satisfied with the facilitator and the videoconferencing experience, and the intervention cost analysis revealed opportunities for cost saving via the use of videoconferenced facilitation. Improvements to the intervention suggested by participants included integrating chronic illness management with medical information systems, involving other health disciplines, and forming networks of community health resources and support services for health providers and patients. This work has demonstrated that although practice facilitation can successfully result in moderate significant improvements in practice behaviour, it is not necessarily singularly effective in all contexts or for all targeted behaviours. A complex practice guideline such as the chronic illness care management model is unlikely to be adopted in the current context of primary care in rural Ontario and as a consequence to have any impact on the health of chronically ill patients without further intervention supports, adaptation, and implementation research undertaken to demonstrate successful execution of chronic illness care management. Alternative care delivery models are required to address barriers and improve the delivery of chronic illness care management.

Nearly two decades of research on translating evidence-based care guidelines into practice has resulted in a considerable body of primary and secondary evidence about guideline implementation strategies and the individual, organizational and environmental challenges associated with closing the evidence to practice gap in primary care. Interventions to improve professional performance are complex and a disentangling of the various independent, intervening and constraining variables is required in order to be able to design and implement interventions that can improve primary care practice performance. The PRECEDE-PROCEED planning model (Green & Kreuter, 1999) provides a step-wise theoretical framework for understanding the complexity of causal relationships among the variables that affect the adoption of evidence-based practice and may assist in the design and implementation of practice-based interventions. Knowledge of an evidence-based practice guideline is important, but a consensus has emerged that having knowledge is rarely sufficient to change practice behaviour. Didactic education or passive dissemination strategies are ineffective, whereas interactive education, reminder systems and multifaceted interventions tailored to the needs of the practice are effective. Outreach or practice facilitation is a proven effective multifaceted approach that involves skilled individuals who enable others, through a range of tailored interventions, to address the challenges in implementing evidence-based care guidelines within the primary care setting. The challenges to implementing evidence-based chronic illness care practice guidelines are thought to be similar to the other contextual, organizational and individual behavioural challenges associated with the uptake of research findings into practice. A multifaceted guideline implementation strategy such as practice facilitation may be well-suited to improving the adoption of these guidelines within rural primary care settings. However, research has not systematically reviewed, through meta-analysis, the published practice facilitation trials to determine overall effects and an implementation research study of practice facilitation that has considered fidelity of implementation within the rural Ontario setting for a complex practice guideline such as chronic illness management has not been done. The systematic review in the thesis incorporated an exploratory meta-analysis of randomized and non-randomized controlled trials of interventions targeted towards implementing evidence-based practice guidelines through practice facilitation, and was conducted to gain an understanding of the overall effect of practice facilitation and the factors that moderate implementation success. The results were the identification of an improvement overtime in the methodological rigour of practice facilitation implementation research based on a critical appraisal of methods, a significant moderate overall effect size of 0.54 (95% CI 0.43 – 0.65) for 19 good quality practice facilitation intervention studies and several significant effect size modifiers; notably, tailoring to the needs of the practice, using multiple intervention components, extending duration, and increasing the intensity of practice facilitation were associated with larger effect sizes. As more practices were assigned to the practice facilitator, the effect diminished. A significant positive association between the number of PRECEDE predisposing, enabling and reinforcing strategies employed by the facilitator and the effect size was detected. The implementation research study utilized mixed methods for data collection as part of an embedded case study of four rural primary care practices to determine the implementation fidelity of the practice facilitation of chronic illness care planning and the factors that impeded and contributed to implementation success. The feasibility of and potential cost savings of practice facilitation via videoconferencing was also implemented for two of the practices. For those practices that successfully implemented care planning, fidelity was achieved for the implementation of care plans. On the other hand, the dosage, duration, component delivery of the practice facilitation intervention was low in comparison to other published studies, and tailoring of the intervention to the practice was inconsistent. Based on the qualitative analysis of physician interviews, the moderating factors for successful implementation were categorized into the broad themes of pessimism and tempered optimism. Pessimistic physicians were unsuccessful at implementation, lacked a willingness to engage and were uncomfortable with the patient-centred approach to chronic illness care. Optimists were positive about the psychosocial, patient-centred assessment aspects of the chronic illness care protocol and provided anecdotes of success in resolving patient problems. However, this was tempered as both pessimists and optimists reflected on the time intensive aspect of the protocol and the unlikelihood of widespread implementation without additional supports. Participating physicians were satisfied with the facilitator and the videoconferencing experience, and the intervention cost analysis revealed opportunities for cost saving via the use of videoconferenced facilitation. Improvements to the intervention suggested by participants included integrating chronic illness management with medical information systems, involving other health disciplines, and forming networks of community health resources and support services for health providers and patients. This work has demonstrated that although practice facilitation can successfully result in moderate significant improvements in practice behaviour, it is not necessarily singularly effective in all contexts or for all targeted behaviours. A complex practice guideline such as the chronic illness care management model is unlikely to be adopted in the current context of primary care in rural Ontario and as a consequence to have any impact on the health of chronically ill patients without further intervention supports, adaptation, and implementation research undertaken to demonstrate successful execution of chronic illness care management. Alternative care delivery models are required to address barriers and improve the delivery of chronic illness care management.

When people do an easy task, and another person is nearby, they tend to do that task better than when they are alone. Conversely, when people do a hard task, and another person is nearby, they tend to do that task less well than when they are alone. This phenomenon is referred to in the social psychology literature as "social facilitation" (the name derives from the "good" side of the effect). Different theories have been proposed to explain this effect. The present study investigated whether people respond to a virtual human the same way they do to a real human. Participants were given different tasks to do that varied in difficulty. The tasks involved anagrams, mazes, modular arithmetic, and the Tower of Hanoi. They did the tasks either alone, in the company of another person, or in the company of a virtual human on a computer screen. As with a human, virtual humans produced the social facilitation effect: for easy tasks, performance in the virtual human condition was better than in the alone condition, and for difficult tasks, performance in the virtual human condition was worse than in the alone condition. Implications for the design of instructional systems as well as other systems involving human-computer interactions are discussed.

The benefits of occupying multiple roles have typically been overlooked. One reason for this oversight is the lack of a well-established scale measuring work-family facilitation. This study developed and validated short, self-report scales of work-to-family facilitation and family-to-work facilitation. Based on conceptualizations of work and family facilitation presented in current research content domains and definitions of the constructs are presented. Work-to-family facilitation is defined as a form of role facilitation in which the experiences in the job, work skills, and emotional gratification from work makes participation in the family easier. Family-to-work facilitation is defined as a form of role facilitation in which the experiences in the family, family skills, and emotional gratification from family makes participation in work easier. Advocated procedures were used to develop the scales and test dimensionality and internal consistency.