Esta pesquisa foi desenvolvida nas lagoas facultativas das estações de tratamento de esgoto dos municípios de Pariquera-Açu (PA) e Jacupiranga (JA), com diferentes configurações, sendo a primeira composta por lagoa anaeróbia seguida de facultativa com chicanas e a outra por lagoa aerada mecanicamente seguida de facultativa. Estas lagoas são consideradas ambientes hipereutróficos, pois apresentam grande quantidade de matéria orgânica oriunda dos esgotos domésticos e industriais. O conhecimento da estrutura da comunidade fitoplanctônica e sua avaliação em diferentes horários do fotoperíodo são relevantes pois estes organismos são responsáveis pela produção de oxigênio necessário para a degradação da matéria orgânica. Esta pesquisa teve como objetivo principal o estudo sazonal e diário da estrutura da comunidade fitoplanctônica e da produção primária nestas lagoas. Para atingir este objetivo foi caracterizada a estrutura da comunidade, em relação a biomassa e diversidade, e determinada a produção primária com incubações in situ através do método do oxigênio dissolvido. O estudo foi realizado em quatro épocas do ano (variação sazonal) no período das 7 às 16 horas, com amostragens de 3 em 3 horas (diária). Não foram observadas variações temporais e espaciais na estrutura da comunidade fitoplanctônica nas duas lagoas facultativas. Apenas as cianobactérias (principalmente a Synechocystis aquatilis e Phormidium sp) e clorofíceas (Kirchneriella lunaris, Chlorella Kessleri, Monoraphidium contortum, Monoraphidium sp, Chlamydomonas sp, Eutetramorus planctonicus e Micractinium pusillum) foram predominantes, nestas lagoas, porém em períodos alternados. Foram observados altos valores de produção primária bruta (15.891 mg\'O IND.2\'/\'M POT.3\'.h em PA; 5.700 mg\'O IND.2\'/\'M POT.3\'.h em JA) na interface água-sedimento das duas lagoas devido, provavelmente, ao padrão de mistura da coluna de água. Foram também observados altos valores de respiração da comunidade (abril, julho e setembro), devido à alta concentração de matéria orgânica presente nestes sistemas e, portanto, maior decomposição, indicando predominância de processos heterotróficos. / This research was developed in facultative ponds of the waste water treatment plant of Pariquera-Açu and Jacupiranga cities with different configurations, being the first composed by anaerobic pond followed by facultative with chicanas and the another composed by aerated mechanic pond followed by facultative. These ponds are considered hypereutrophic environments because it shows a large quantity of organic matter derived from the domestic and industrial sewage. The knowledge of phytoplanktonic community structure and its evaluation in different times of photoperiod are relevant since such organisms was responsible by production of oxygen necessary to the degrade of organic matter. This research had the main goal the seasonal and daily study of the phytoplanktonic community and the primary production in these ponds. To reach this objective the structure of the community was characterized in relation to biomass and density, and determined the primary production with incubations in situ through the dissolved oxygen method. The study was made in four periods of the year (seasonal variation) between the 7 a.m. to 4 p.m., with samplings of 3-to-3 hours (daily). It was not observed temporal and spatial variations in the phytoplanktonic community structure in the facultative ponds. Only cyanobacteria (mainly Synechocystis aquatilis and Phormidium sp) and chlorophycea (Kirchneriella lunaris, Chlorella Kessleri, Monoraphidium contortum, Monoraphidium sp, Chlamydomonas sp, Eutetramorus planctonicus and Micractinium pusillum) were predominant in these ponds, however, in alternated periods. It was observed high rates of gross primary production (15891 mg\'O IND.2\'/\'M POT.3\'.h, PA; 5700 mg\'O IND.2\'/\'M POT.3\'.h, JA) in the interface water-sediment of both ponds due, probably, to the standard of mixed conditions of water column. It was also observed high rates of community respiration (April, July and September), due to the high concentration of organic matter present in these systems and, therefore, greater decomposition, indicating predominance of heterotrophic processes.

>Magister Scientiae - MSc / Different chlorhexidine (CHX) preparations and formulations are available in local markets. Some preparations contain anti-discoloration systems (ADS), additional antimicrobials like cetylpyridinium chloride (CPC), or alcohol. The aim of this study was to compare the antimicrobial efficacies of 3 different CHX preparations (Corsodyl®, Curasept® and GUM® Paroex®)

Submitted by Angela Maria de Oliveira (amolivei@uepg.br) on 2018-05-23T18:24:02Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Karine Marcondes da Cunha.pdf: 2703816 bytes, checksum: 0fd02c84ec3249f309d4ac15f15ca275 (MD5) / Made available in DSpace on 2018-05-23T18:24:02Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Karine Marcondes da Cunha.pdf: 2703816 bytes, checksum: 0fd02c84ec3249f309d4ac15f15ca275 (MD5) Previous issue date: 2018-05-24 / Um grande volume de resíduos sólidos orgânicos (RSO) e de tabaco de cigarro contrabandeado tem sido produzido. Geralmente esses resíduos são descartados em aterros sanitários, incinerados ou dispostos diretamente no solo, tornando-se contaminantes ao meio ambiente. A compostagem é um processo viável para tratar esses resíduos, mas no Brasil tem sido pouco utilizada, por necessitar de manejo constante, além de grandes espaços e distantes de residências. Estudos recentes em reatores domésticos facultativos estarão sendo desenvolvidos e se apresentam como uma alternativa viável para compostagem. Possuem a vantagem de poder estar próximos a residências, sem a necessidade de grandes espaços e de manejo. Com o intuito de atender locais com maior produção de RSO, como restaurantes, pequenas indústrias, condomínios, entre outros, o presente trabalho teve como objetivo estudar o uso de um reator facultativo com capacidade de 2000 litros para o tratamento de RSO, cepilho e tabaco de cigarros contrabandeados. Para o monitoramento do processo foram realizadas análises físico-químicas (pH, temperatura, umidade e razão C/N), biológicas (teste de germinação e indicativos de patógenos e patógenos) e espectroscópicas (ultra violeta visível - UV-Vis e infra - vermelho IV). Além disso, foi investigada a influência de duas diferentes razões iniciais C/N no processo de compostagem, a razão C/N 28 e 21. As análises da razão iniciais C/N e os testes de fitotoxicidade mostraram que o composto alcançou sua maturidade em um período de 120 dias. Por meio das análises espectroscópicas de UV-Vis e IV constatou-se a eficiência, indicando a degradação de compostos de estrutura mais simples e a formação de compostos humificados. Nas análises microbiológicas e de metais, observou-se que as concentrações estão de acordo com as legislações Ministério da Agricultura e Abastecimento - MAPA (2014) e Diretrizes para qualidade do composto - Canadá CCME (2005) para o composto orgânico. As diferentes razões iniciais C/N avaliadas não mostraram variações significativas na qualidade do composto final. Assim, concluiu-se que a proposta de tratamento dos resíduos estudados por meio dos reatores facultativos de 2000 litros atingiu à qualidade esperada, com formação de composto humificado e sem a presença de contaminantes, demonstrando então eficiência. / A large volume of organic solid waste (RSO) and smuggled cigarette tobacco has been produced. Usually these wastes are discarded in landfills, incinerated or disposed directly in the soil, becoming contaminants to the environment. Composting is a viable process to treat these wastes, but in Brazil it has been little used, because it requires constant management, as well as large spaces and distant from residences. Recent studies in facultative domestic reactors are being developed and presented as a viable alternative to composting. They have the advantage of being close to residences, without the need of large spaces and handling. The objective of this study was to study the use of a facultative reactor with a capacity of 2000 liters for the treatment of RSO, brush and tobacco smuggled cigarettes. The physical-chemical analyzes (pH, temperature, humidity and C / N ratio), biological (germination test and pathogen and pathogen indicative) and spectroscopic analyzes (ultraviolet visible - UV-Vis and infra-red IV). In addition, the influence of two different initial C / N ratios in the composting process, the C / N ratio 28 and 21, was investigated. Initial C / N ratio analyzes and phytotoxicity tests showed that the compound reached maturity in period of 120 days. By means of spectroscopic analyzes of UV-Vis and IV the efficiency was verified, indicating the degradation of compounds of simpler structure and the formation of humified compounds. In the microbiological and metal analyzes, it was observed that the concentrations are in accordance with the legislation for the organic compound. The different initial C / N ratios evaluated did not show significant variations in the quality of the final compound. Thus, it was concluded that the proposed treatment of the residues studied by means of the optional 2000 liter reactors reached the expected quality, with the formation of a humidified compound and without the presence of contaminants, thus demonstrating efficiency.

Premier arrivé, premier servi. Que cela s’appelle un privilège ou un droit de préférence particulier, il reste inacceptable au regard des règles substantielles du droit positif français.Cette répartition des sommes saisies sacrifie, pour des raisons procédurales, une règle importante à savoir l’égalité entre les créanciers.Cette étude propose une solution intermédiaire entre le droit civil et le droit des voies d’exécution. La proposition consiste à donner à tous les créanciers, qui ont obtenu par leur vigilance des titres exécutoires avant le premier acte de saisie, la possibilité d’associer le premier saisissant dans la répartition des sommes saisies dans le cadre d’une saisie attribution.La combinaison de l’effet attributif immédiat de la saisie avec une durée de quinze jours,pendant laquelle les créanciers titulaires des titres exécutoires viennent en concours sous réserve des causes légitimes de préférences et des privilèges, aboutit à un double résultat. Le recouvrement des créances reste rapide et simple et l’égalité entre les créanciers sera respectée / Whether it is called a privilege or a private preference right, the ‘first come first served rule’remains debatable given the substantive rules of the French positive law. Primarily for procedural reasons, the norm of seizures distribution undermines a crucial principle, namely equality among creditors.This study proposes an intermediary solution between civil law and law of enforcement procedures. It advocates granting all creditors, who have obtained their enforcement orders before the first act of seizure, the possibility of associating with the first executioner the distribution of the seized money.The combined effect of immediate attribution of the seizure with duration of fifteen days,during which all creditors holding enforceable securities are subject to competition, and taking into account other legitimate preferences and privileges, leads to a double result: a simple and fast method of debt recovery without undermining the principle of equality between creditors.

Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-10-08T19:32:10Z No. of bitstreams: 2 Dissertação - Carlos de Melos e Silva Neto - 2013.pdf: 671422 bytes, checksum: 7774a52856834c1b8270fa6218029c99 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2014-10-09T11:23:41Z (GMT) No. of bitstreams: 2 Dissertação - Carlos de Melos e Silva Neto - 2013.pdf: 671422 bytes, checksum: 7774a52856834c1b8270fa6218029c99 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2014-10-09T11:23:41Z (GMT). No. of bitstreams: 2 Dissertação - Carlos de Melos e Silva Neto - 2013.pdf: 671422 bytes, checksum: 7774a52856834c1b8270fa6218029c99 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2013-09-16 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Pollinators provide an essential service to the ecosystem and bring numerous benefits to society, through its role in the production of food in agriculture and the conservation of biological diversity. In tomato, anthers are poricidal, thus the release of pollen grains requires the presence of pollinators that vibrate these anthers. Thus, pollinators of tomatoes are actually bees that perform buzz pollination. Here, we observed aspects of floral biology, quantity and viability of pollen grains and ovules, and pollinators behavior and richness. To evaluate the effects of pollination in the amount of pollen on the stigmas, we selected 37 plants and bagged an inflorescence and let another available to pollinators. Each marked inflorescence had three stigmas removed the pollen grains on their surfaces were quantified. For comparison of fruit production, fruit development were followed in inflorescences bagged and not bagged for 40 days, and then the fruits were counted, weighed, measured, and had their seeds counted. The anthesis is at 6:30 am. The flower remains open until 6:00 pm, closing and reopening in the next day at the same time, lasting up to 73 hours. The greatest amount of pollen in the anther is in the early hours of the flower opening, with reduction and stabilization of the number of pollen grains around noon, coinciding with increased pollinator visitation. We found 25 species of bees in pollinating tomato crops. The abundance of bees coinciding with hours of availability and depletion of pollen suggests efficient removal of pollen from the anthers by the native bees The amount of pollen on the stigma of flowers available to pollinators was higher than on the stigma of bagged flowers. Fruit production was higher in open inflorescences than in bagged inflorescences. Native bees pollinate tomato flowers, increasing the pollen load on the stigma and fruit production. / Os polinizadores fornecem um serviço essencial ao ecossistema e trazem inúmeros benefícios à sociedade, através do seu papel na produção de alimento na agricultura e na conservação da diversidade biológica. No tomateiro, as anteras têm abertura poricida, assim, para liberação dos grãos de pólen é necessária a presença de polinizadores efetivos que realizam a vibração dessas anteras. Sendo assim, os polinizadores de fato dos tomateiros são abelhas que realizam polinização vibrátil. Na realização deste trabalho, foram observados aspectos da biologia floral como duração da flor, a quantidade e viabilidade de grãos de pólen e óvulos, além dos aspectos ecológicos dos polinizadores, como observação e coleta dos visitantes florais. Para avaliar os efeitos da polinização na quantidade de pólen do estigma, selecionamos 37 plantas, ensacamos uma inflorescência e deixamos outra disponível aos polinizadores. De cada inflorescência marcada, retiramos três estigmas e quantificamos os grãos de pólen em suas superfícies. Para a comparação da produção de frutos, acompanhamos o desenvolvimento dos frutos nas inflorescências ensacadas e não ensacadas durante 40 dias, e posteriormente os frutos foram contados, pesados, medidos e tiveram suas sementes contadas. A antese floral inicia às 6h30 da manhã. A flor permanece aberta até às 18h, fechando e no dia seguinte reabrindo no mesmo horário, durando até 73h. A maior oferta de pólen é nas primeiras horas da abertura da flor, com redução e estabilização do número de grãos de pólen por volta do meio dia, coincidindo com o aumento da visitação dos polinizadores. Foram encontradas 25 espécies de abelhas polinizadoras nos cultivos de tomateiro. A abundância das abelhas coincidindo com os horários de disponibilidade e esgotamento do pólen sugere eficiência na retirada dos grãos de pólen das anteras, muitos dos quais se aderem provavelmente ao estigma da própria flor, ocorrendo o posterior desenvolvimento dos frutos. A quantidade de grãos de pólen no estigma das flores disponíveis para os polinizadores foi maior do que no estigma das flores ensacadas. A produção de frutos foi maior em inflorescências não ensacadas que em inflorescências ensacadas. Os frutos não ensacados apresentaram mais sementes que os ensacados. As abelhas nativas polinizam as flores do tomateiro, aumentando a carga de pólen no estigma e a produção de frutos.

Les indemnités de fin de contrat, qu’elles résultent de la loi ou de la volonté contractuelle, sont nombreuses et variées et malgré leur importance pratique aucune étude d’ensemble ne leur avait été consacrée en droit privé. Cette thèse se propose de les étudier conjointement pour en dresser une typologie et en déterminer le régime juridique. La première partie traite de la variété des indemnités, qu’elles soient légales (VRP, agents commerciaux, gérants-mandataires, bail commercial ou rural) ou conventionnelles (indemnité d’anéantissement unilatéral ou de dédit). Il est démontré que l’indemnité de fin de contrat représente le prix d’une liberté contractuelle retrouvée permettant la sauvegarde de l’équilibre économique du contrat. Les contreparties sont en revanche différentes. Elles constituent un encadrement du droit d’anéantir le contrat unilatéralement ou offrent le droit de se dédire, c’est-à-dire de ne pas exécuter le contrat en tout ou partie. La seconde partie est consacrée au régime juridique des indemnités. Il en résulte une liberté contractuelle orientée in melius au profit du créancier de l’indemnité légale. Ainsi que le constat d’un contrôle judiciaire insuffisant s’agissant des indemnités conventionnelles. Ce faisant, il est proposé des modifications législatives tant concernant les indemnités légales par l’octroi d’une indemnité de fin de contrat au profit des distributeurs-revendeurs, que s’agissant des indemnités conventionnelles par la consécration explicite de la validité du dédit conventionnel ainsi que l’élaboration d’un contrôle judiciaire du montant des indemnités conventionnelles excessives sur le modèle de la clause pénale. / Termination benefits, whether as a result of the law or the contractual will, are numerous and varied and despite their practical importance no comprehensive study had been dedicated to this topic in private law. This thesis proposes to study them jointly to establish a typology and to determine the legal regime. The first part deals with the variety of allowances, whether they are legal or conventional. It is shown that the termination benefit represents the price of a restored contractual freedom with a view to securing a fair balance of interests. The counterparties are, on the other hand, different. They provide a legal framework for the right to annihilate the contract unilaterally or offer the right to withdraw, that is to say, not to execute the contract in whole or in part. The second part is devoted to the legal system of allowances. The result is a contractual freedom directed in melius to the benefit of the creditor of the legal indemnity. As well as the finding of insufficient judicial review in respect of conventional allowances. In this regard, legislative amendments are proposed, both with regard to statutory allowances by granting a termination payment to reseller distributors, as well as conventional allowances by the legal consecration of the validity of conventional withdrawal as well as the development of a judicial review of the amount of excessive conventional allowances, built on the model of the penalty clause.

Breathing is essential for human life, yet tens of millions of people in the U.S. alone suffer from lung diseases. With each breath, lungs are exposed to the external environment. Inhaled air first passes through the trachea, bronchi, and finally the bronchioles before it reaches the alveoli where gases are exchanged. A barrier of epithelial cells protects the airways. In addition, epithelial glands also secrete protein-rich fluids onto the airway surfaces to help maintain sterility. Injury, disease, or other factors can damage these cells, and regiospecific stem cells (SCs) can divide to replace them. However, many important details about lung SCs are still unknown. For example, what processes control SC division? How do region-specific SCs differ from one another? And how does disease or injury impact SC biology? We found that some processes that regulate lung development also control adult SC division following injury. We show that SCs from airway glands give rise to surface epithelial cell types and glandular cell types. In contrast, surface SCs only generated surface cell types. Finally, we identify a type of cell in the glands that can regenerate surface cell types after severe injury. These studies provide new insights into the neighborhoods in which SCs reside in the large airways and processes that control their contribution to airway repair following injury. Overall, this research provides important new insights into adult SC biology and conditions affecting lung health.

<p>To further investigate the strength of the association and the relative advantages of the association to both organisms, several manipulation experiments were set up. A cage experiment set up in the shallow subtidal zone showed that the coralline survived equally well without the winkle and did therefore not require the winkle or its empty shell for survival. A second controlled laboratory aquarium experiment was designed under both fluorescent (rich in blue light) and incandescent light (rich in red light) to ascertain whether the coralline had a preference for O. sinensis over the similar O. tigrina. This experiment was inconclusive as no recruitment was obtained under either of the light regimes. A third laboratory experiment was designed to determine whether the extra coralline weight had any possible advantage to the winkle, particularly against predation from the rock lobster Jasus lalandii. Results suggested that there were no apparent advantages to the winkle bearing the extra coralline load as adult O. sinensis bearing the coralline alga (3.7 &plusmn / 2.2 winkles 24hr-1) were equally prone to predation than those lacking the coralline (2.3 &plusmn / 1.9 winkles 24hr-1) (p = 0.184). Observations suggested instead that the convoluted nature of the coralline may indeed have promoted predation. We ultimately deduced that the high occurrence of the coralline on the shells of O. sinensis was probably due to the substantial overlap in the niches of the two organisms. This conclusion was supported by the high densities of juvenile O. sinensis combined with the high percent cover abundance of the coralline in intertidal rockpools. Understanding sexual reproduction in coralline algae as well as the life cycle of the winkle, ultimately provided insight into the postulated life cycle of this coralline-winkle association...</p>

Anoxia is an environmental stress that few air-breathing vertebrates can tolerate for more than a few minutes before extensive neurodegeneration occurs. Some facultative anaerobes, including the freshwater western painted turtle Chrysemys picta bellii, are able to coordinately reduce ATP demand to match reduced ATP availability during anoxia, and thus tolerate prolonged insults without apparent detriment. To reduce metabolic rate, turtle neurons undergo channel arrest and spike arrest to decrease membrane ion permeability and neuronal electrical excitability, respectively. However, although these adaptations have been documented in turtle brain, the mechanisms underlying channel and spike arrest are poorly understood. The aim of my research was to elucidate the cellular mechanisms that underlie channel and spike arrest and the neuroprotection they confer on the anoxic turtle brain. Using electrophysiological and fluorescent imaging techniques, I demonstrate for the first time that: 1) the α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR) undergoes anoxia-mediated channel arrest; 2) delta opioid receptors (DORs), and 3) mild mitochondrial uncoupling via mitochondrial ATP-sensitive K+ channels result in an increase in cytosolic calcium concentration and subsequent channel arrest of the N-methyl-D-aspartate receptor, preventing excitotoxic calcium entry, and 4) reducing nitric oxide (NO) production; 5) the cellular concentration of reactive oxygen species (ROS) decreases with anoxia and ROS bursts do not occur during reoxygenation; and 6) spike arrest occurs in the anoxic turtle cortex, and that this is regulated by increased neuronal conductance to chloride and potassium ions due to activation of γ–amino-butyric acid receptors (GABAA and GABAB respectively), which create an inhibitory electrical shunt to dampen neuronal excitation during anoxia. These mechanisms are individually critical since blockade of DORs or GABA receptors induce excitotoxic cell death in anoxic turtle neurons. Together, spike and channel arrest significantly reduce neuronal excitability and individually provide key contributions to the turtle’s long-term neuronal survival during anoxia. Since the turtle is the most anoxia-tolerant air-breathing vertebrate identified, these results suggest that multiple mechanisms of metabolic suppression acting in concert are essential to maximizing anoxia-tolerance.

Anoxia is an environmental stress that few air-breathing vertebrates can tolerate for more than a few minutes before extensive neurodegeneration occurs. Some facultative anaerobes, including the freshwater western painted turtle Chrysemys picta bellii, are able to coordinately reduce ATP demand to match reduced ATP availability during anoxia, and thus tolerate prolonged insults without apparent detriment. To reduce metabolic rate, turtle neurons undergo channel arrest and spike arrest to decrease membrane ion permeability and neuronal electrical excitability, respectively. However, although these adaptations have been documented in turtle brain, the mechanisms underlying channel and spike arrest are poorly understood. The aim of my research was to elucidate the cellular mechanisms that underlie channel and spike arrest and the neuroprotection they confer on the anoxic turtle brain. Using electrophysiological and fluorescent imaging techniques, I demonstrate for the first time that: 1) the α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR) undergoes anoxia-mediated channel arrest; 2) delta opioid receptors (DORs), and 3) mild mitochondrial uncoupling via mitochondrial ATP-sensitive K+ channels result in an increase in cytosolic calcium concentration and subsequent channel arrest of the N-methyl-D-aspartate receptor, preventing excitotoxic calcium entry, and 4) reducing nitric oxide (NO) production; 5) the cellular concentration of reactive oxygen species (ROS) decreases with anoxia and ROS bursts do not occur during reoxygenation; and 6) spike arrest occurs in the anoxic turtle cortex, and that this is regulated by increased neuronal conductance to chloride and potassium ions due to activation of γ–amino-butyric acid receptors (GABAA and GABAB respectively), which create an inhibitory electrical shunt to dampen neuronal excitation during anoxia. These mechanisms are individually critical since blockade of DORs or GABA receptors induce excitotoxic cell death in anoxic turtle neurons. Together, spike and channel arrest significantly reduce neuronal excitability and individually provide key contributions to the turtle’s long-term neuronal survival during anoxia. Since the turtle is the most anoxia-tolerant air-breathing vertebrate identified, these results suggest that multiple mechanisms of metabolic suppression acting in concert are essential to maximizing anoxia-tolerance.