Ketohexokinase (KHK), in an adenosine triphosphate (ATP) dependent reaction, catalyzes the first reaction in fructose metabolism, which converts the furanose form of D-fructose into fructose-1-phosphate. This enzyme has become a target for pharmacological development against fatty liver and metabolic syndrome. KHK exists in two isoforms, A and C, which differs by alternative splicing of exon 3 which encodes 45 out of 298 amino acids. Normally KHK exists as a homodimer and is comprised of an alpha/beta domain interlocking with a β-clasp domain. For KHK-C, there appear to be at least two conformations of the β-clasp domain. Previous work on KHK-A reveals it does not adopt the same conformations. A structure of the mouse KHK-A in its unliganded form is solved and shows that these two conformations also exist for KHK-A. Furthermore, this property is conserved across species. While crystals of human KHK-A in its unliganded form were grown, a structure was not achieved. However, unpublished structures of human KHK-A in its unliganded form also shows different conformations in β-clasp domain when in juxtaposition with the same enzyme complex with ligands. Defining the role of conformational changes in KHK-A is important, because this isozyme has been reported to have a role in cancer metastasis.

This is a quantitative content analysis that dives deep into the Fresh and Fit podcast channel on YouTube. The study uses the framing theory and the gender theory. We wonder why other creators choose some of the video material from the channel Fresh and Fit podcast and make it their “best of” edition. All videos from other creators made between the years 2022 and 2023 have been selected for the study and through this a total selection has been made. To each video the top 5 top comments are analyzed to see which tonality dominates. Through gender theory, the comment field will be examined and thus focus on the dominant tonality.The reason for not including videos presented by Fresh and Fit themselves is to exclude their own opinions and only concentrate the study on the choices of other creators. This is interesting as larger YouTube profiles more easily get further sharing by other people's accounts, who in different ways comment or cut out material that is further presented to the audience. It may affect different major accounts in different ways as their own material may be angled in ways that are beyond the bigger channels control. In addition, the creators can get help to spread their material more easily through the interest that exists in the larger channel's already established following, which can further result in the smaller creator's account growing even larger. This is the case for Fresh and Fit, which over the course of four years has grown so large that a number of different creators have chosen to use their material and republish it. So too satisfy curiosity, we have chosen to dive deeper into how other creators take liberties with Fresh and Fit material published on YouTube. The results show that it turned out that the creators were mainly interested in bringing up topics of conversation that dealt with women's behavior. In addition to this, the study concluded that those who mainly got to speak were the hosts together with female guests, but that male guests were given more space of their own to express themselves.

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