A study has been made of the external conversion spectrum of gamma-rays of less than 200 kev. A set of semi-empirical curves relating the photoelectric peak height to the gamma-ray intensity has been established using antimony radiators. These results will be discussed. A method of preparing thin beta sources will also be described. / Thesis / Master of Science (MSc)

As the world's nuclear reactors approach the end of their originally planned lifetimes and seek license extensions, which would allow them to operate for another 20 years, accurate information regarding neutron radiation exposure is more important than ever. Structural components such as the reactor pressure vessel (RPV) become embrittled by neutron irradiation, reducing their capability to resist crack growth and increasing the risk of catastrophic failure. The current dosimetry approaches used in these high flux environments do not provide real-time information. Instead, radiation dose is calculated using computer simulations, which are checked against dose readings that are only available during refueling once every 1.5-2 years. These dose readings are also very expensive, requiring highly trained technicians to handle radioactive material and operate specialized characterization equipment. This dissertation describes the development of a novel neutron radiation dosimeter based on carbon nanotubes (CNTs) that not only provides accurate real-time dosimetry, but also does so at very low cost, without the need for complex instrumentation, highly trained operators, or handling of radioactive material. Furthermore, since this device is based on radiation damage rather than radioactivation, its readings are time-independent, which is beneficial for nuclear forensics. In addition to development of a novel dosimeter, this work also provides insight into the particularly under-investigated topic of the effects of neutron irradiation of carbon nanotubes. This work details the fabrication and characterization of carbon nanotube based neutron and gamma radiation dosimeters. They consist of a random network of CNTs, sealed under a layer of silicon dioxide, spanning the gap between two electrodes to form a conductive path. They were fabricated using conventional wafer processing techniques, making them intrinsically scalable and ready for mass production. Electrical properties were measured before and after irradiation at several doses, demonstrating a consistent repeatable trend that can be effectively used to measure dose. Changes to the microstructure were investigated using Raman spectroscopy, which confirmed that the changes to electrical properties are due to increasing defect concentration. The results outlined in this dissertation will have significant impacts on both the commercial nuclear industry and on the nanomaterials scientific community. The dosimeter design has been refined to the point where it is nearly ready to be deployed commercially. This device will significantly improve accuracy of RPV lifetime assessment while at the same time reducing costs. The insights into the behavior of CNTs in neutron and gamma radiation environments is of great interest to scientists and engineers studying these nanomaterials. / Ph. D.

Proton Compton scattering has been studied from 220 to 330 MeV at 90 degree center of mass scattering angle with the polarized photon beam at LEGS. Compton scattering is an important probe of the nucleon structure and its excited states. Polarization degrees of freedom reveal more information than unpolarized observables. This experiment measured, for the first time, the polarized cross sections of proton Compton scattering up to the Δ resonance. The parallel and perpendicular cross sections were measured at the same time. All the photons were tagged so that the energy dependent systematic errors are small. The measured unpolarized cross section is above the lower bound from unitary at all energies. The cross section asymmetry is obtained as a function of energy for the first time. The unpolarized cross section is compared with previous data and with calculations from the fixed-t dispersion model, the isobar model and the finite energy dispersion model. Although all these calculations agree fairly well with unpolarized cross section data, the photon asymmetry data show that the isobar model contains serious defects. The ratio of polarized cross sections, dσ_⫫/dσ_⟂, is compared with a model independent result. The results from the present experiment can be used in the calculation of the E2/M1 ratio in the N — Δ transition, which is an important signature of the tensor interaction between quarks. / Ph. D.

This thesis summarizes a senior project on the Active Galactic Nucleus (AGN) Markarian 421 (Mrk 421). Observations of Gev and TeV flux with Fermi Large Area Telescope (LAT) and High Energy Stereoscopic System (H.E.S.S.) were compared with previous reports and publications of flux analyses of the gamma-ray emission from Mrk 421. Power laws with exponential cutoffs made consistent fits to most SEDs in the GeV and TeV bands.

Title: Study of Cosmic-Ray Acceleration in Extragalactic Objects Author: Stanislav tefnik Department: Institute of Particle and Nuclear Physics Supervisor: RNDr. Dalibor Nosek, Dr. Abstract: This thesis deals with production processes of cosmic γ-rays in astro- physical objects and methods of their detection. Possible interactions leading to the emission of high energy γ-rays in the active galaxy Centaurus A are discussed in this context. Cherenkov Telescope Array is presented as a new experiment fo- cused on the detection of air showers initiated by cosmic γ-rays. Cherenkov light of air showers is studied in the simulations done by CORSIKA simulation tool. Method of data analysis within the framework of Cherenkov telescopes is descri- bed and performed on the dataset of the active galaxy PKS 2155-304. The results include statistical tests of γ-ray source presence and its time variability. Keywords: Cosmic rays, gamma rays, astroparticle physics, high energy astro- physics, acceleration, CTA experiment

A radiação gama é uma das tecnologias aplicadas para a esterilização de sistemas de embalagens contendo produtos para a saúde. No processo de esterilização, é fundamental que as propriedades das embalagens sejam mantidas. Neste estudo, duas amostras de embalagens comerciais, no formato de envelope compostas por papel grau cirúrgico de um dos lados e filme plástico laminado do outro, foram irradiadas com raios gama com doses de 25 kGy (taxa de dose de 1,57 kGy/h) e 50 kGy (taxa de dose de 1,48 kGy/h). Uma das amostras de embalagem era constituída por papel de fibras de coníferas e filme plástico laminado de poli(tereftalato de etileno) (PET)/polietileno (PE) e a outra por papel de fibras de coníferas e de folhosas e filme plástico laminado de poli(tereftalato de etileno) (PET)/polipropileno (PP). Os efeitos da radiação nas propriedades físicas e químicas dos papéis e dos filmes plásticos, assim como nas propriedades da embalagem foram estudados. O papel foi o material mais sensível à radiação, sendo a alvura, o pH e as resistências ao rasgo, ao arrebentamento e à tração os parâmetros nos quais foram observadas maiores modificações, em ambas as amostras. Todavia, dos dois tipos de papel, o efeito foi mais pronunciado para a amostra com fibras de conífera e de folhosas. A porosidade dos papéis foi alterada com 50 kGy. No caso dos filmes plásticos, a propriedade com maior modificação foi a resistência à tração, em ambas as amostras. No caso das embalagens, a irradiação diminuiu a resistência da selagem. Os efeitos observados para a dose de 50 kGy foram em média mais pronunciados quando comparados com as modificações nos valores das amostras tratadas com 25 kGy, que é a dose usualmente empregada para esterilização de produtos para a saúde. A dosimetria deste estudo foi realizada nos materiais irradiados com 25 kGy, 40 kGy e 50 kGy, demonstrando sua importância à medida que a variação média para as três doses estudadas foi de 20 %. / Gamma radiation is one of the technologies applied for the sterilization of packaging systems containing products for health. During sterilization process it is critical that the properties of packages are maintained. In this study two samples of commercial pouch packaging comprised of surgical grade paper on one side and the other side multilayer plastic film were irradiated with gamma rays. The following doses were applied 25 kGy (1,57 kGy/h) and 50 kGy (1,48 kGy/h). One packaging sample was paper formed by softwood fibers and multilayer plastic film based on poly(ethylene terephthalate) (PET)/polyethylene (PE). The second type of paper sample was made by a mixture of softwood and hardwood fibers and multilayer plastic film based on polyethylene terephthalate (ethylene) (PET)/polypropylene (PP). The effects of radiation on the physical and chemical properties of papers and multilayer plastic films, as well as the properties of the package were studied. The paper was the more radiation sensitive among the studied materials and radiation effects were more pronounced at brightness, pH, tearing resistance, bursting strength and tensile strength. Nonetheless, worst comparatively effects were noted on the sample made by a mixture of softwood and hardwood fibers. The porosity of paper was enhanced by 50 kGy. In the case of plastic films, radiation effects on tensile strength was the most pronounced property for both samples. In the case of the packaging the sealing resistance decreased with radiation. The effects observed for the treatment at 50 kGy were more pronounced when compared to 25 kGy. This last is the dose which is usually applied to sterilize health products. A dosimetry study was performed during irradiation at 25 kGy, 40 kGy and 50 kGy and its importance may be reported by the average dose variation 20 %.

A engenharia de tecidos e a medicina regenerativa possuem como objetivo principal o restabelecimento morfológico/funcional de tecidos e órgãos lesionados com a utilização de células, matrizes celulares e células tronco, controlando as respostas imunológicas/bioquímicas promovidas pelo organismo. Adicionalmente, a ciência dos materiais busca desenvolver biomateriais biocompatíveis que não promovam reações imunológicas indesejadas e proporcionem o reestabelecimento das funções do tecido/órgão. Polímeros de origem natural destacam-se como biomateriais por assemelharem-se a macromoléculas biológicas, similaridade com a matriz extracelular, menor possibilidade de estimulação de inflamação crônica e baixa ou ausência de toxicidade. O presente trabalho teve como objetivo desenvolver matrizes macromoleculares originadas do óleo de soja epoxidado (OSE), analisando a relação estrutura química/atividade biológica das matrizes macromoleculares para uso como biomaterial na engenharia de tecidos. A síntese do OSE foi efetuada pela rota oleoquímica, cuja eficiência foi determinada por espectroscopia de infravermelho e o rendimento da reação de 85% determinado por ressonância magnética nuclear de prótons. A partir da análise por calorimetria exploratória diferencial, detectou-se uma diminuição da temperatura de transição vítrea do polímero do óleo de soja (POSE) em relação ao OSE, sugerindo aumento do crescimento das cadeias poliméricas do POSE. Através da análise termogravimétrica, foi possível definir o perfil de degradação do OSE, com degradação em duas etapas, e do POSE, que degrada em apenas uma etapa e demonstra maior estabilidade térmica do POSE pelo aumento das interações moleculares. A reticulação e a hidrofilicidade do POSE foram promovidas com a adição de metacrilato de 2-hidroxietila (HEMA) à formulação por enxertia do monômero pela irradiação gama. Os resultados obtidos identificaram aumento da estabilidade mecânica, da gelificação e da absorção de água com o aumento do conteúdo de HEMA. Por fim, o grau de cristalinidade estimado para esses polímeros enxertados com HEMA de 27,5% foi definido através da difratometria de raios-X. A segunda etapa caracterizou-se pelo (i) desenvolvimento de POSEs com a enxertia de HEMA nas proporções OSE/HEMA 90:10 e 65:35 com irradiação por raios gama nas doses de 50 e 100kGy, (ii) caracterização físico-química dos POSE-HEMA e (iii) análise biológica desses materiais. Através da espectroscopia de infravermelho, pode-se detectar as regiões epoxidadas do POSE, assim como o sucesso da enxertia do monômero HEMA em todas as concentrações e doses de radiação utilizadas. Através da calorimetria exploratória diferencial, calculou-se a energia de ativação (Ea) dos polímeros. A cristalinidade dos materiais foi definida por difratometria de raios-X, mostrando caráter amorfo do material, bem como um pequeno incremento na porcentagem da cristalinidade com o aumento da intensidade das doses de radiação durante a síntese e um decréscimo dessa cristalinidade com o aumento na concentração de HEMA. A análise da citotoxicidade das amostras mostrou a ausência de toxicidade dos POSE-HEMA, confirmando a eficiência das lavagens dos polímeros para retirada de resíduos do processamento. A análise da hemocompatibilidade mostrou ausência de adesão de plaquetas e os testes de crescimento celular nas matrizes foram positivos. Através dos resultados obtidos nesta pesquisa, pôde-se concluir pelo potencial de utilização dos POSE-HEMA na engenharia de tecidos. / Tissue engineering and regenerative medicine have as main objective the morphologic/functional reestablishment of injured tissues and organs using cells, scaffolds, stem cells and control of immunological/biochemical responses promoted by the body. In addition, materials science seeks to develop biocompatible biomaterials that do not promote unwanted immune responses and provide the re-establishment of the functions of the tissue/organ. Polymers of natural origin stand out as biomaterials to resemble biological macromolecules, similarity to the extracellular matrix, reduced chance of inflammation and chronic pacing low or no toxicity. This study aimed the development of macromolecular arrays originated from epoxidized soybean oil (OSE), analyzing the relationship between the chemical structure/biological activity of the macromolecular arrays for use as biomaterials in tissue engineering. The synthesis of OSE was performed through the oleochemical route, whose efficiency was determined by infrared spectroscopy and the reaction yield of 85%, determined by nuclear magnetic resonance spectroscopy. From the analysis by differential scanning calorimetry, it was detected a decrease of the glass transition temperature of the epoxidized soybean oil polymer (POSE) compared with OSE, suggesting an increase of the growth of polymer chains of POSE. Thermogravimetric analysis was performed to define the OSE degradation profile, which degrades in two steps. The POSE degrades in just one step and shows higher thermal stability by the increased molecular interactions. The hydrophilicity and crosslinking of POSE was promoted by the addition of 2-hydroxyethyl methacrylate (HEMA) with the monomer grafting by gamma irradiation. The results showed an increased mechanical stability, gelation and water absorption with the HEMA content increasing. Finally, the degree of crystallinity for such polymers grafted with HEMA was 27.5%, estimated by X-ray diffractometry. The second stage was characterized by (i) developing POSEs with the grafting of HEMA in the proportions OSE / HEMA 90:10 and 65:35 irradiated by gamma rays at doses of 50 and 100kGy, (ii) physico-chemical characterization of POSE-HEMA and (iii) analysis of biological materials. By infrared spectroscopy, it was detect the epoxidized regions of POSE, as well as the successful grafting of the monomer HEMA concentrations with all radiation doses. By differential scanning calorimetry, the activation energy was calculated (Ea) of the polymers. The crystallinity of the material was defined by X-ray diffraction, showing tendency of amorphous material as well as a small percentage of the increase in crystallinity with increasing intensity of radiation doses during this synthesis and a decrease in crystallinity with the increasing concentration of HEMA. The analysis of the samples did not show cytotoxicity on POSE-HEMA and confirmed the efficiency of polymer washings to remove the processing waste. The analysis of hemocompatibility showed any platelet adhesion and the cell growth on the scaffolds was positive. From the results obtained in this research, we concluded by the potential use of POSE-HEMA in tissue engineering.

Die vorliegende Dissertation analysiert Beobachtungen des Vela-Pulsars mit dem H.E.S.S.-Observatorium und präsentiert damit den ersten Nachweis gepulster Strahlung mit H.E.S.S. Die signifikante Detektion (11.4 s) oberhalb einer bis jetzt unerreichten Energieschwelle von lediglich 15 GeV wurde mittels von 24 h Beobachtungsdaten erzielt, die mit dem im Jahr 2012 in Betrieb genommenen CT5-Teleskop aufgezeichnet wurden. Nach dem Krebs-Pulsar ist damit der Vela-Pulsar der zweite Pulsar, von dem sehr hochenergetische Gammastrahlung nachgewiesen wurde. Die Spektren von Pulsaren im Bereich der sehr hochenergetischen Gammastrahlung sind von besonderem Interesse, da sie Einblicke in einen bisher fast komplett unerforschten Energiebereich erlauben. Der experimentelle Nachweis stellt eine besondere Herausforderung dar, da die Rate der Gammastrahlen gemäß einem Potenzgesetz mit der Energie abfällt und die resultierenden Zählraten klein sind. Daher werden neue gewichtete Testgrößen eingeführt, um die Sensitivität für den Nachweis schwacher Pulsationen für zukünftige Beobachtungen von Pulsaren zu verbessern. Die Testgrößen sind Erweiterungen von bereits existierenden ungewichteten Verfahren und anwendbar auf beliebige diskrete Daten, in denen ein Puls oder mehrere Pulse erwartet werden. Sie sind leistungsfähig für den Fall unbekannter Phasenprofile und erzielen bei Standardgewichtung eine 10 %-ige Steigerung der Detektionssignifikanz. Die optimierte Analysekette wurde zur Detektion des Vela-Pulsars im Energiebereich von 15 GeV bis 125 GeV eingesetzt. Der phasengemittelte integrierte Energiefluss beträgt 4.29_(-1.02)^(+1.14)_stat _(-3.31)^(+5.50)_sys × 10^(-11) erg cm^(-2) s^(-1) und wird durch einen einzelnen Puls verursacht. Das Profil des Pulses kann durch eine asymmetrischen Lorentz-Funktion mit einer schmaleren abfallenden Flanke beschrieben werden. Der Photonfluß fällt gemäß einem Potenzgesetz mit Index -5.39 mit der Energie ab. Dies ist in guter Übereinstimmung mit einem theoretischen Annular-Gap-Emissionsmodell. / The analysis of the first pulsar to be detected with the H.E.S.S. array is presented. The high significance detection at the 11.4 s level down to a hitherto unachievable 15 GeV energy threshold is a result of 24 h of observations of the Vela pulsar with the new CT5 telescope introduced into the array in 2012. This is only the second pulsar to be detected in the very high energy (VHE) ?-ray regime. Of particular interest are the VHE spectra of pulsars as this is an almost completely unexplored energy domain. This does however entail smaller signals as the rate of ?-rays generally drops according to a power law function. New weighted statistical tests are introduced to improve the sensitivity to weak pulsations for use with future observations of pulsars. These tests are modifications of currently used tests and are applicable to any discrete data in which a single or many pulses are expected; they are powerful when the phase profile is unknown and with a basic weighting provide up to a 10 % boost in detection significance. An optimised analysis chain contributed to the detection of the Vela pulsar with phase averaged energy flux in the energy range (15,125) GeV of 4.29_(-1.02)^(+1.14)_stat _(-3.31)^(+5.50)_sys × 10^(-11) erg cm^(-2) s^(-1). The single pulse is characterised with an asymmetric Lorentzian function with a narrower trailing edge. The photon flux falls as a power law with index -5.39 which is moderately consistent with an Angular Gap emission model but represents a steeper drop in emission than that measured with the Fermi-LAT. The emission efficiency of the Vela pulsar in the VHE band is evaluated as 0.0025 %, which is comparable to that of the Crab pulsar in the same energy regime measured with the MAGIC telescope array. Comparisons to other candidate VHE pulsars are also drawn. A weak constraint is placed on the energy at which Lorentz invariance violation occurs in terms of the Planck energy as E_LIV>10^(-4) E_P.

Das Thema dieser Arbeit ist die fuer das HEGRA Experiment entwickelte Rekonstruktions-Algoritmen, die Geometry und Energie von hochenergetischen kosmischen Gamma-Strahlen zu rekonstruieren, in die H.E.S.S. software Umgebung zu implementieren und das nicht-gepulste Energie-Spektrum des Krebsnebels zwischen Energien von 300 GeV und 20 TeV zu bestimmen. Dafuer wurden die ersten stereoskopischen Daten von Oktober und November 2003 mit einer 3 Teleskope-Konfiguration des H.E.S.S. Systems der Phase-I verwendet. Die Phase-I des H.E.S.S. Systems wurde im Dezember 2003 fertiggestellt, nachdem das vierte Teleskop in Betrieb genommen wurde. Die Rekonstruktionsalgorithmen wurden mit Monte Carlo Simulationen fuer die vollstaendige Phase-I des Teleskop-Systems getestet. Die Aufloesung fuer die rekonstruierte Richtung und Energie der einzelnen gamma-Ereignisse sind 0.15 Grad und 14 Prozent bei 45 Grad Zenitwinkel. Die Daten des Krebsnebels, die eine Wobble-Versetzung von +-0.5 Grad and +-1.0 Grad haben und die im Zenitwinkel-Bereich von 45 Grad bis 50 Grad fuer 4 Stunden beobachtet wurden, geben ein Signal von 50 Standardabweichungen. Das differentielle Energiespektrum des Krebsnebels zwischen 450 GeV und 20 TeV nach den Schnitten ist (dN / dE) = (3.37+-0.47)*10^{-11}*E^{-2.59+-0.12} cm^{-2} s^{-1} TeV^{-1}. Der integrierte Fluss oberhalb 1 TeV ist (2.11+-0.29)*10^{-11} cm^{-2} s^{-1}. Diese Resultate sind konsistent mit Messungen anderer Experimente, speziell von HEGRA und Whipple. Die Resultate stimmen mit den Erwartungen der synchroton self-Compton Modelle fuer den TeV Emissionbereich ueberein. Das magnetische Feld in der Region, wo die TeV gamma-Strahlen vermutlich entstehen, wird zu 0.18+-0.01 mG bestimmt. Die Resultate dieser Arbeit zeigen die Leistungsfaehigkeit des H.E.S.S. Teleskop-Systems. / The goal of this thesis is to implement the methods developed for the HEGRA experiment to reconstruct the geometry and energy of the air-showers induced by the cosmic high-energy gamma rays into the software environment of the H.E.S.S. experiment. Furthermore, using the implemented algorithms, a search for the unpulsed emission is aimed in the energy range between 300 GeV and 20 TeV from the Crab Nebula using the first stereoscopic data taken during October and November 2003 with the 3 telescope configuration of the H.E.S.S. array in Phase-I. The Phase-I of the H.E.S.S. array was completed in December 2003 by the addition of the fourth telescope. By testing the reconstruction algorithms of a complete Phase-I H.E.S.S. array with Monte Carlo simulations, it is found that the resolution of the reconstructed direction and energy of a gamma-ray event from a zenith angle of 45 degrees is around 0.15 degrees and 14 percent, respectively. The data on the Crab Nebula including runs with wobble offset of +-0.5 degrees and +-1.0 degrees is collected at zenith angles from 45 degrees to 50 degrees for a total of 4 hours and gives a background subtracted signal of about 50 standard deviations. The differential energy spectrum of the unpulsed gamma-ray emission from the Crab Nebula is found to be (dN / dE) = (3.37+-0.47)*10^{-11}*E^{-2.59+-0.12} cm^{-2} s^{-1} TeV^{-1} between 450 GeV and 20 TeV after all cuts. The integral flux above 1 TeV is (2.11+-0.29)*10^{-11} cm^{-2} s^{-1}. These results are consistent with the results published by other experiments, in particular HEGRA and Whipple. The results agree well with the expectation from synchrotron self-Compton models for TeV emission range. The magnetic field in the region, where TeV gamma rays are produced, is found to be 0.18+-0.01 mG. This result agrees with the magnetic field values deduced by the models. The results obtained for the Crab Nebula in this thesis demonstrate the performance of the H.E.S.S. array.

A engenharia de tecidos e a medicina regenerativa possuem como objetivo principal o restabelecimento morfológico/funcional de tecidos e órgãos lesionados com a utilização de células, matrizes celulares e células tronco, controlando as respostas imunológicas/bioquímicas promovidas pelo organismo. Adicionalmente, a ciência dos materiais busca desenvolver biomateriais biocompatíveis que não promovam reações imunológicas indesejadas e proporcionem o reestabelecimento das funções do tecido/órgão. Polímeros de origem natural destacam-se como biomateriais por assemelharem-se a macromoléculas biológicas, similaridade com a matriz extracelular, menor possibilidade de estimulação de inflamação crônica e baixa ou ausência de toxicidade. O presente trabalho teve como objetivo desenvolver matrizes macromoleculares originadas do óleo de soja epoxidado (OSE), analisando a relação estrutura química/atividade biológica das matrizes macromoleculares para uso como biomaterial na engenharia de tecidos. A síntese do OSE foi efetuada pela rota oleoquímica, cuja eficiência foi determinada por espectroscopia de infravermelho e o rendimento da reação de 85% determinado por ressonância magnética nuclear de prótons. A partir da análise por calorimetria exploratória diferencial, detectou-se uma diminuição da temperatura de transição vítrea do polímero do óleo de soja (POSE) em relação ao OSE, sugerindo aumento do crescimento das cadeias poliméricas do POSE. Através da análise termogravimétrica, foi possível definir o perfil de degradação do OSE, com degradação em duas etapas, e do POSE, que degrada em apenas uma etapa e demonstra maior estabilidade térmica do POSE pelo aumento das interações moleculares. A reticulação e a hidrofilicidade do POSE foram promovidas com a adição de metacrilato de 2-hidroxietila (HEMA) à formulação por enxertia do monômero pela irradiação gama. Os resultados obtidos identificaram aumento da estabilidade mecânica, da gelificação e da absorção de água com o aumento do conteúdo de HEMA. Por fim, o grau de cristalinidade estimado para esses polímeros enxertados com HEMA de 27,5% foi definido através da difratometria de raios-X. A segunda etapa caracterizou-se pelo (i) desenvolvimento de POSEs com a enxertia de HEMA nas proporções OSE/HEMA 90:10 e 65:35 com irradiação por raios gama nas doses de 50 e 100kGy, (ii) caracterização físico-química dos POSE-HEMA e (iii) análise biológica desses materiais. Através da espectroscopia de infravermelho, pode-se detectar as regiões epoxidadas do POSE, assim como o sucesso da enxertia do monômero HEMA em todas as concentrações e doses de radiação utilizadas. Através da calorimetria exploratória diferencial, calculou-se a energia de ativação (Ea) dos polímeros. A cristalinidade dos materiais foi definida por difratometria de raios-X, mostrando caráter amorfo do material, bem como um pequeno incremento na porcentagem da cristalinidade com o aumento da intensidade das doses de radiação durante a síntese e um decréscimo dessa cristalinidade com o aumento na concentração de HEMA. A análise da citotoxicidade das amostras mostrou a ausência de toxicidade dos POSE-HEMA, confirmando a eficiência das lavagens dos polímeros para retirada de resíduos do processamento. A análise da hemocompatibilidade mostrou ausência de adesão de plaquetas e os testes de crescimento celular nas matrizes foram positivos. Através dos resultados obtidos nesta pesquisa, pôde-se concluir pelo potencial de utilização dos POSE-HEMA na engenharia de tecidos. / Tissue engineering and regenerative medicine have as main objective the morphologic/functional reestablishment of injured tissues and organs using cells, scaffolds, stem cells and control of immunological/biochemical responses promoted by the body. In addition, materials science seeks to develop biocompatible biomaterials that do not promote unwanted immune responses and provide the re-establishment of the functions of the tissue/organ. Polymers of natural origin stand out as biomaterials to resemble biological macromolecules, similarity to the extracellular matrix, reduced chance of inflammation and chronic pacing low or no toxicity. This study aimed the development of macromolecular arrays originated from epoxidized soybean oil (OSE), analyzing the relationship between the chemical structure/biological activity of the macromolecular arrays for use as biomaterials in tissue engineering. The synthesis of OSE was performed through the oleochemical route, whose efficiency was determined by infrared spectroscopy and the reaction yield of 85%, determined by nuclear magnetic resonance spectroscopy. From the analysis by differential scanning calorimetry, it was detected a decrease of the glass transition temperature of the epoxidized soybean oil polymer (POSE) compared with OSE, suggesting an increase of the growth of polymer chains of POSE. Thermogravimetric analysis was performed to define the OSE degradation profile, which degrades in two steps. The POSE degrades in just one step and shows higher thermal stability by the increased molecular interactions. The hydrophilicity and crosslinking of POSE was promoted by the addition of 2-hydroxyethyl methacrylate (HEMA) with the monomer grafting by gamma irradiation. The results showed an increased mechanical stability, gelation and water absorption with the HEMA content increasing. Finally, the degree of crystallinity for such polymers grafted with HEMA was 27.5%, estimated by X-ray diffractometry. The second stage was characterized by (i) developing POSEs with the grafting of HEMA in the proportions OSE / HEMA 90:10 and 65:35 irradiated by gamma rays at doses of 50 and 100kGy, (ii) physico-chemical characterization of POSE-HEMA and (iii) analysis of biological materials. By infrared spectroscopy, it was detect the epoxidized regions of POSE, as well as the successful grafting of the monomer HEMA concentrations with all radiation doses. By differential scanning calorimetry, the activation energy was calculated (Ea) of the polymers. The crystallinity of the material was defined by X-ray diffraction, showing tendency of amorphous material as well as a small percentage of the increase in crystallinity with increasing intensity of radiation doses during this synthesis and a decrease in crystallinity with the increasing concentration of HEMA. The analysis of the samples did not show cytotoxicity on POSE-HEMA and confirmed the efficiency of polymer washings to remove the processing waste. The analysis of hemocompatibility showed any platelet adhesion and the cell growth on the scaffolds was positive. From the results obtained in this research, we concluded by the potential use of POSE-HEMA in tissue engineering.