Orientador: João Domingos Rodrigues / Banca: Elizabeth Orika Ono / Banca: Camilo Lázaro Medina / Banca: José Antônio Proença Vieira de Moraes / Banca: Susete Aparecida de Barros Cardoso / Resumo: O presente trabalho objetivou avaliar a aplicação de reguladores vegetais (tratamento de semente e via foliar) em limoeiro 'Cravo' (Citrus limonia Osbeck) visando o encurtamento do tempo de produção do porta-enxerto. O experimento foi dividido em duas etapas, sendo a primeira realizada no laboratório de Análise de Sementes, Departamento de Produção Vegetal - Agricultura da Faculdade de Ciências Agronômicas - Botucatu, SP, determinando-se a taxa de embebição das sementes. Após este procedimento, realizou-se o teste de germinação das sementes após tratamento com os produtos (testemunha, ácido giberélico GA3 10 mg 0,2 L-1, ácido giberélico GA3 20 mg 0,2 L-1, cinetina 10 mL 0,2 L-1, piraclostrobina + metil tiofanato 0,03 mL L-1, cinetina + ácido giberélico + ácido indolilbutírico 30 mL 0,2 L-1, ácido giberélico GA4+7 + 6-benziladenina 1 mL 0,2 L-1, ácido giberélico GA4+7 + 6-benziladenina 2,12 mL 0,2 L-1, cloreto de chlormequat 0,2 mL 0,2 L-1 e cloreto de chlormequat 0,2 mL 0,2 L- 1 + ácido giberélico GA3 20 mg 0,2 L-1. A segunda etapa do experimento foi realizada no viveiro ELPA Mudas Cítricas, localizado na cidade de Botucatu, SP. Após resultado da taxa de embebição as sementes foram mantidas em contato com os tratamentos e semeadas em tubetes com substrato de fibra de coco. Foram empregados dez tratamentos aplicados via foliar, mensalmente: testemunha, ácido giberélico GA3 50 mg L-1, ácido giberélico GA3 100 mg L-1, cinetina 50 mL L-1, piraclostrobina + metil tiofanato 0,18 mL L-1, cinetina + ácido giberélico + ácido indolilbutírico 150 mL L-1, ácido giberélico GA4+7 + 6-benziladenina 5 mL L-1, ácido giberélico GA4+7 + 6-benziladenina 10 mL L-1, cloreto de chlormequat... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The aim of this project was to evaluate the plant growth regulator application (seed and leaves) in rangpur lime (Citrus limonia Osbeck) on rootstock production in a shorter period of time. The experiment was divided into two steps; the first one was carried in the Seed Analysis Laboratory, Crop Science Department - College of Agricultural Sciences - Botucatu, SP, in order to analyze the inhibition. Afterwards, the germination test was done after the contact of the seeds with the treatments (control, gibberellic acid GA3 (10 mg L-1) 10 mg 0,2 L-1, gibberellic acid GA3 (10 mg L-1) 20 mg 0,2 L-1, kinetin (0,4 mg L-1) 10 mL 0,2 L-1, pyraclostrobin + thiophanate methyl (50 g L-1 + 450 g L-1) 0,03 mL L-1, kinetin (90 mg L-1) + gibberellic acid (50 mg L-1) + indolebutyric acid (50 mg L-1) 30 mL 0,2 L-1, gibberellic acid GA4+7 (19 mg L-1) + 6-benzyladenine (19 mg L-1) 1 mL 0,2 L-1, gibberellic acid GA4+7 (19 mg L-1) + 6-benzyladenine (19 mg L-1%) 2,12 mL 0,2 L-1, chlormequat chloride (100 g L-1) 0,2 mL 0,2 L-1 and chlormequat chloride (100 g L-1) 0,2 mL 0,2 L-1 + gibberellic acid GA3 (10 mg L-1) 20 mg 0,2 L-1. The second part of the experiment was carried out in ELPA Citric Seedlings' Nursery, in Botucatu, SP. After the inhibition test, the seeds were kept in contact with the treatments and sowed in the coconut fiber substrate. Ten treatments were provided monthly by foliar application: control, gibberellic acid GA3... (Complete abstract click electronic access below) / Doutor

Study aim is to study endogenic and exogenic factors preconditioning morphogenesis of linseed flax in vitro. Linseed flax studies in vitro were carried out at the Laboratory of Genetic – Biotechnological of Lithuanian University of Agriculture in 2004-2006. Factors which influence morphogenetic capability of linseed flax cultivars ‘Lirina’, ‘Barbara’ and ‘Szaphir’ in tissue culture were investigated. Two different types of explants – stem segments and hypocotyls – of the three genotypes were cultivated in nutrient media differing in their macro and micro salts concentration, vitamins and different levels of growth regulators. Our results showed that intensity of morphogenesis was affected not only by exogenous growth regulators, but also by the type of explant and genotype. Hypocotyls-derived callus gave the better results than that stem-segment-derived callus on all tested media. Four-weeks-old callus showed the maximum shoot regeneration frequency. The best morphogenetic capabilities were demonstrated for cultivar ‘Szaphir’. The largest number of shoots was produced by all the cultivars on MSB5 nutrient medium supplemented with cytokinin 2iP (2.0 mg 1-1). The master’s final work comprises 50 pages, including 3 tables, 10 figures and 66 literature references.

Darbo objektas – našlaičių (Viola x wittrockiana Gams) daigai apdoroti skirtingos koncentracijos augimo reguliatoriaus CCC tirpalais (0,15 %, 0,30 % ir 0,45 %), skirtingais būdais (laistant ir purškiant) ir dažniais (kas 7 dienas ir 14 dienų). Darbo metodai: Tirti našlaičių biometriniai rodikliai pagal J. Vaidelio (2005) sudarytą metodiką. Matavimai atlikti balandžio 20 d., augalams pasiekus žydėjimo tarpsnį (BBCH 60 - 65), kurio metu vertinta našlaičių daigų žiedų dydis, žiedstiebių ir lapkočių ilgiai, kerelių aukštis bei plotis, apskaičiuotas žiedpumpurių ir lapų skaičius bei nustatyta žydėjimo pradžia. Darbo rezultatai. Panaudotas augimo reguliatorius CCC, turėjo įtakos našlaičių (Viola x witrockiana Gams) daigų kokybiniams rodikliams. Didinant tirpalo koncentraciją (0,15 %, 0,30 %, 0,45 %) nustatyta lapų, žiedpumpurių skaičiaus didėjimo tendencija ir vėlino žydėjimo pradžią. Skirtinga koncentracija statistiškai patikimai veikė (mažino) našlaičių kerelių aukštį, žiedstiebio ir lapkočio ilgį bei kerelių skersmenį. Patikimai daugiausiai lapų užaugino, našlaitės, kurios buvo laistytos kas 14 dienų lyginant su kontroliniu variantu. Žydėjimo trukmę labiausiai įtakojo laistymas kas 14 d. didžiausios koncentracijos tirpalu, tuo tarpu anksčiausiai žydėti pradėjo daigai, purkšti kas 7 dienas, 0,15 % CCC tirpalu. Didžiausią įtaką lapkočio ir žiedstiebio ilgiui turėjo laistymas kas 14 d., 0,45 % tirpalu. Didinant koncentraciją, tačiau ribojant purškimų skaičių, našlaičių daigai... [toliau žr. visą tekstą] / Object of the work: pansy (Viola x wittrockiana Gams) seedlings treated with different concentrations of growth regulator CCC solutions (0,15 %, 0,30 % and 0,45 %) in different ways (spraying and watering) and frequency (every 7 and 14 days ). Methods of the work: pansy biometric parameters were measured using the methodology by J. Vaidelys (2005). Measurements were carried out on 20th April. When plants reached the flowering stage (BBCH 60 - 65) during which assessment of pansy size of flowers, and the stalks lengths, seedling height and diameter, calculated the number of flower buds and leaves and set the beginning of flowering. The results of the work: growth regulator CCC, had an impact on pansy’s (Viola x witrockiana Gams) seedling quality parameters. Increasing the concentration of the solution (0,15%, 0,30%, 0,45%), amount of leaves and buds increasing trend. Different concentrations were significantly reduced pansy seedling height, stalk length and diameter of the plant, but did not affected the flower size. The biggest impact was 0,45 % concentration of the solution. Reliably produced the most leaves, pansy, which watered every 14 days compared with the control treatment. Flowering time was much influenced by watering every 14 days with the highest concentration of a solution, while the earliest flowering started when pansy was spray every 7 days with 0,15 % CCC solution. The biggest impact on the stalk and the length was watering every 14 days with 0,45% solution... [to full text]

Thesis (MScAgric (Horticulture))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: Creasing, also known as albedo breakdown, is a preharvest disorder that affects the albedo of citrus fruit causing creases on the surface of the fruit. It is a recurrent problem in Navel and Valencia oranges and can cause individual orchard losses which often exceed 50%. Although the contributing factors are known, the physiological basis of creasing development is unresolved and the current control measures do not prevent creasing satisfactory. Hence, better control measures and further understanding of the physiology of creasing development is required. The objective of this two-year study was to determine if the position of fruit in a tree, light and carbohydrate manipulation techniques, and albedo mineral nutrients influence creasing development. Furthermore, the most effective application timing of gibberellic acid (GA3) with the least negative effect on fruit rind colour development and the effectiveness of cytokinins, other products and different root biostimulants to reduce creasing incidence were evaluated. The position of fruit in the tree and light influenced the development of creasing and the distribution of mineral nutrients in the albedo. Creasing incidence was higher on the south side than on the north side of the tree and fruit from the inside sub-sectors had a greater creasing incidence compared to fruit from the outside sub-sectors. The shady part of outside fruit was more creased compared to the sunny part of the fruit and covering fruit with brown paper bags increased creasing severity. The light manipulation techniques used on the leaves and fruit increased the nitrogen (N), phosphorus (P), potassium (K) and manganese (Mn) concentrations in the albedo and differences in the albedo mineral nutrients amongst the sub-sectors evaluated were observed, but creasing severity or creasing incidence was not significantly correlated with the albedo mineral concentrations at harvest. Albedo mineral concentrations earlier in the season may play a role in creasing development, as creasing severity was significantly correlated with copper (Cu), K, and Mn concentrations in the albedo during stage II of fruit development. Creasing incidence and albedo mineral concentrations were not affected by any of the carbohydrate manipulation techniques used in this study. The incidence and severity of creasing was significantly reduced, with a minor negative effect on fruit rind colour development, by the application of GA3, from mid November to mid January. Localised fruit application of CPPU [N-(2-chloro-4-pyridyl)-N-phenylurea], MaxCel (6- Benzyladenine) and CPPU in combination with calcium after physiological fruit drop reduced the incidence and severity of creasing, although creasing incidence was not significantly different from the control. The application of Messenger®, AVG (aminoethoxyvinylglycine) and different root biostimulants did not reduce creasing incidence. The results showed that cytokinins could reduce creasing incidence and justify further studies on application and uptake efficiency. The use of different root biostimulants are not recommended, but it is suggested that treatment effects may be more pronounced over a longer period. / AFRIKAANSE OPSOMMING: Kraakskil is ‘n vooroes abnormalitiet wat die albedo van sitrusvrugte affekteer, deur krake op die oppervlak van vrugte te veroorsaak. Dit is ‘n algemene probleem in Navel en Valencia lemoene en kan boordverliese van tot 50% of soms hoër veroorsaak. Alhoewel die bydraende faktore bekend is, is die fisiologiese basis van kraakskil ontwikkeling onopgelos en die beskikbare beheermaatreëls is nie bevredigend nie. Dus, beter beheermaatreëls en ‘n beter begrip van die fisiologie van kraakskil ontwikkeling is nodig. Die doel van die twee-jaar studie was om te bepaal of die posisie van vrugte in ‘n boom, lig en koolhidraat manipulasie tegnieke en minerale elemente in die albedo, kraakskil ontwikkeling beïnvloed. Die mees effektiewe toedieningstyd van gibberelliensuur (GA3) sonder ‘n negatiewe effek op vrugkleur is bepaal en die effektiwiteit van sitokiniene, ander produkte en verskillende wortel biostimulante om kraakskil voorkoms te verminder, is geëvalueer. Die posisie van vrugte in ‘n boom en lig het kraakskil ontwikkeling en die verspreiding van minerale element in die albedo beïnvloed. Kraakskil voorkoms was hoër aan die suidekant van die boom as aan die noordekant en vrugte in die binnekant van die boom het ‘n groter kraakskil voorkoms as vrugte in die buitekant van die boom gehad. Die skadukant van buitevrugte het meer kraakskil gehad as die sonkant en die toemaak van vrugte met ‘n bruin papiersak het die graad van kraaksil verhoog. Die lig manipulasie tegnieke wat op die blare en vrugte gebruik is, het die stikstof (N), fosfaat (P), kalium (K) en mangaan (Mn) konsentasies in die albedo verhoog en verskille in die albedo minerale elemente tussen sub-sektore is waargeneem, maar betekenisvolle korrelasies is nie tussen die graad en voorkoms van kraakskil en die albedo minerale element konsentrasies by oestyd waargeneem nie. Albedo minerale element konsentrasies vroeër in die seisoen mag ‘n rol speel by kraakskil ontwikkeling, omdat die graad van kraakskil betekenisvol gekorreleer was met albedo koper (Cu), K, en Mn konsentrasies tydens fase II van vrugontwikkeling. Kraakskil voorkoms en albedo minerale element konsentrasies is nie deur enige van die koolhidraat manipulasie tegnieke geaffekteer nie. Die voorkoms en graad van kraakskil is betekenisvol verlaag, met ‘n geringe negatiewe effek op vrugkleur, deur die toediening van GA3 vanaf mid November tot mid Januarie. Gelokaliseerde vrugtoedienings van CPPU [N-(2-chloro-4-piridiel)-N-phenielureum], MaxCel (6- Bensieladenien) en CPPU saam met kalsium na fisiologiese vrugval het die voorkoms en graad van kraakskil verlaag, alhoewel kraakskil voorkoms nie betekenisvol van die kontrole verskil het nie. Die toediening van Messenger®, AVG (amino etoksievinielglisien) en veskillende wortel biostimulante het nie kraakskil voorkoms verlaag nie. Die resultate het getoon dat sitokiniene kraakskil voorkoms kan verlaag en verdere studies op die toediening en opname effektiwiteit word aanbeveel. Die gebruik van verskillende wortel biostimulante word nie aanbeveel nie, maar die effek behoort meer sigbaar te wees na ‘n langer periode van behandeling.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2018-07-09T19:37:57Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) clara_almeida_guerra.pdf: 1004414 bytes, checksum: 6688d13a6216ca1b6380300e85cdd209 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-07-18T13:16:18Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) clara_almeida_guerra.pdf: 1004414 bytes, checksum: 6688d13a6216ca1b6380300e85cdd209 (MD5) / Made available in DSpace on 2018-07-18T13:16:18Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) clara_almeida_guerra.pdf: 1004414 bytes, checksum: 6688d13a6216ca1b6380300e85cdd209 (MD5) Previous issue date: 2017 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O objetivo deste trabalho foi desenvolver uma metodologia de propaga??o in vitro de Lychnophora pohlii Sch. Bip., envolvendo as etapas de alongamento, enraizamento e pr?-aclimata??o de pl?ntulas cultivadas in vitro. Na etapa de alongamento, no primeiro experimento avaliou-se o efeito dos reguladores de crescimento ANA + BAP, ANA + TDZ e GA3 combinados com os meios MS e MS/2 na altura m?dia e percentual de calos. Ainda nessa etapa, no experimento dois e tr?s, utilizou-se o meio MS e concentra??es de GA3 (0; 0,5; 1; 2 e 4 mg L-1) e ANA (0,1; 0,3; 0,6; e 0,9 mg L-1), e avaliou-se a altura m?dia, percentual de enraizamento, n?mero m?dio de ra?zes e percentual de calos. Na etapa de enraizamento, nos experimento quatro e cinco foram testadas concentra??es de ANA e AIB (1,0 e 2 mg L-1) e quatro tempos de perman?ncia dos explantes no meio (7, 14, 21e 28 dias) e as avalia??es foram realizadas semanalmente quanto ao percentual de enraizamento, n?mero m?dio de raiz, altura m?dia e percentual de calos. Tamb?m nessa etapa, para os experimentos seis e sete, os tratamentos foram constitu?dos de dois tipos de meio (MS/2 e WPM/2) e concentra??es de ANA e AIB (0,1 e 0,5 mg L-1) e avaliados o percentual de enraizamento, n?mero m?dio de ra?zes, altura m?dia e percentual de calos. Na etapa de pr?-aclimata??o, as pl?ntulas foram transplantadas para copos pl?sticos (200 cm3) contendo vermiculita? autoclavada e os tratamentos foram constitu?dos por pl?ntulas cobertas com saco de polietileno intacto, pl?ntulas cobertas com saco de polietileno perfurado e aus?ncia de cobertura, onde se avaliou o percentual de sobreviv?ncia, altura m?dia, comprimento m?dio da raiz principal, mat?ria fresca da parte a?rea, mat?ria fresca da raiz, mat?ria seca da parte a?rea e mat?ria seca da raiz. Na fase de alongamento, a maior m?dia de altura foi verificada em explantes cultivados em meio MS, suplementado com ANA + BAP. A combina??o de ANA + TDZ aumentou o percentual de calo. As concentra??es de 0,9 e 0,1 mg L-1 proporcionaram a maior e menor n?mero m?dio de raiz por tratamento. O menor percentual de calo foi observado na concentra??o de 0,1 mg L-1 de ANA. Na fase de enraizamento a concentra??o de 1,0 mg L-1 de ANA proporcionou maior m?dia de altura e menor percentual de calo e na concentra??o de 2,0 mg L-1 se observou a menor m?dia de altura e o maior percentual de calo. O tempo de perman?ncia de 21 dias possibilitou a maior m?dia de altura dos explantes tratados com ANA e AIB. A concentra??o de 2,0 mg L-1 de AIB contribuiu com o maior n?mero m?dio de ra?zes. O meio WPM/2 suplementado com 0,5 mg L-1 de ANA obteve o maior n?mero m?dio de ra?zes. A maior m?dia para a altura foi encontrada nos explantes cultivados em meio WPM/2. A concentra??o de 0,1 mg L-1 de ANA proporcionou o menor percentual de calo. A concentra??o de 0,5 mg L-1 de AIB proporcionou os maiores valores para o percentual de enraizamento, n?mero m?dio de ra?zes e percentual de calo. Na fase de pr?-aclimata??o, o maior percentual de sobreviv?ncia foi observado no tratamento em que as pl?ntulas foram cobertas com sacos de polietileno intacto. As pl?ntulas cobertas com sacos de polietileno perfurado expressaram maior altura m?dia, comprimento m?dio da raiz, mat?ria fresca e seca da parte a?rea. Nessas condi??es os explantes mostraram-se responsivos quanto aos tratamentos testados, denotando boas perspectivas quanto ao cultivo in vitro dessa esp?cie. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / The objective of this work was to develop an in vitro propagation methodology of Lychnophora pohlii Sch. Bip. including the stretching, rooting and pre-acclimatization of seedlings grown in vitro stages. In the stretching stage, the effect of the growth regulators ANA + BAP, ANA + TDZ and GA3 combined with the MS and MS/2 media were evaluated at the mean height and callus percentage. At this stage, the effect of concentrations of GA3 (0, 0.5, 1, 2 and 4 mg L-1) and ANA (0.1, 0.3, 0, 6, and 0.9 mg L-1) were evaluated at the mean height, rooting percentage, mean number of roots and percentage of callus. In the rooting stage, ANA and IBA concentrations (1.0 and 2 mg L-1) and four dwell times of the explants in the medium (7, 14, 21 and 28 days) were tested and rooting percentage, root mean number, mean height and callus percentage were evaluated weekly. Also in this step, the treatments were composed of two types of medium (MS/2 and WPM/2) and ANA and IBA concentrations (0.1 and 0.5 mg L-1). In the pre-acclimation stage, the seedlings were transplanted to plastic cups (200 cm3) containing autoclaved vermiculite?. The treatments were composed by seedlings covered with intact polyethylene bag, seedlings covered with perforated polyethylene bag and seedling uncovered It was evaluated the percentage of survival, mean height, mean length of the main root, fresh shoot matter, fresh root matter, shoot dry matter and root dry matter. In the elongation phase, the highest mean height was verified in explants grown in MS medium, supplemented with ANA + BAP. The combination of ANA + TDZ increased the callus percentage. Concentrations of 0.9 and 0.1 mg L-1 provided the highest and lowest root mean number per treatment, respectively. The lowest percentage of callus was observed for the concentration of 0.1 mg L-1 of ANA. In the rooting stage, the concentration of 1.0 mg L-1 of ANA provided a higher average height and a lower percentage of callus. At the concentration of 2.0 mg L-1, the lowest mean height and the highest percentage of callus were observed. The dwell time of 21 days allowed the highest average height of the explants treated with ANA and AIB. The concentration of 2.0 mg L-1 of AIB contributed with the highest average number of roots. The WPM/2 medium supplemented with 0.5 mg L-1 of ANA obtained the highest mean number of roots. The highest mean height was found in the explants grown on WPM/2 medium. The concentration of 0.1 mg L-1 of ANA provided the lowest percentage of callus. The concentration of 0.5 mg L-1 of IBA provided the highest values for rooting percentage, mean number of roots and percentage of callus. In the pre-acclimatization stage, the highest percentage of survival was observed in the treatment in which the seedlings were covered with bags of intact polyethylene. Seedlings covered with perforated polythene bags expressed higher mean height, root mean length, fresh and dry shoot matter. In these conditions, the explants were responsive to the treatments tested, indicating good perspectives regarding the in vitro cultivation of this species.

Submitted by Claudia Rocha (claudia.rocha@udesc.br) on 2018-02-21T13:43:00Z No. of bitstreams: 1 PGPV15MA185.pdf: 1457517 bytes, checksum: 4293d4dda1796f82f86a5921ddb2d618 (MD5) / Made available in DSpace on 2018-02-21T13:43:00Z (GMT). No. of bitstreams: 1 PGPV15MA185.pdf: 1457517 bytes, checksum: 4293d4dda1796f82f86a5921ddb2d618 (MD5) Previous issue date: 2015-10-29 / PROMOP / In years when conditions are unfavorable for pollination and when the intensity of flowering is small, you may need to improve the fruit set. The use of growth regulators can be a strategy to be adopted in order to ensure good fruit set. The Thidiazuron is a phenylurea with cytokinin action, when it is applied at low concentrations during cell division it can increase fruit set and fruit size. The aim of this study was to evaluate the effect of Thidiazuron concentration in fruit set and fruit quality of apple ‘Royal Gala’, grown in mild winter conditions. The experiment was arranged during cycles 2011/2012, 2012/2013 and 2013/2014 in Fraiburgo, SC, in the Royal Gala apple trees in Marubakaido rootstock M9 with filter. In all evaluation cycles the experimental design was a randomized block with factorial design (6x3x2) with six concentrations of TDZ, two application forms and three years of evaluation, composed of twelve treatments with six replications, the experimental unit was formed by one plant. The TDZ concentrations of each treatment were applied in two seasons. The first application was performed on pink bud stage (E2) and the second application in full bloom stage (F2). The variables: fruit set (%), return of flowering (%), number and weight of fruits per plant, average fruit bulk (g), average fruit length, average fruit diameter and length/diameter of the fruit (C/D), average number of seeds, caliber by classes: ≤135; and between 136-165 ≥180. Red coloration of the skin of the fruit were evaluated in three classes: percentage of fruits with a red color covering less than 50% of the surface; percentage of fruits with a red color covering 50% to 80% of the surface; and percentage of fruits with red color covering more than 80% of the whole surface. Were determined the mineral content of N, P, K, Ca, Mg and their relationship, flesh firmness, starch degradation and soluble solids content. The data were submitted to analysis of variance and the significant variables was performed the analysis of orthogonal contrasts. Significant data also were adjusted in the regression equations to evaluate the behavior of the variables with increasing concentration. We also used Pearson correlation analysis to check for possible relationships between the different attributes evaluated. The TDZ is effective even at low concentrations, and it increases production and fruit set of apple especially when there are unfavorable weather problems during pollination period. TDZ reduces the ripening of fruits. There is a reduction in the percentage of red color covering the skin of the fruit and number of seeds per fruit in plants that received the application of TDZ. The levels of minerals present in the flesh keeps within the sufficiency range independent of the concentration of TDZ applied / Em anos em que as condições forem desfavoráveis à polinização e quando a intensidade da floração for pequena, pode ser necessário melhorar a frutificação efetiva. A utilização de fitorreguladores pode ser uma estratégia a ser adotada a fim de garantir uma boa frutificação efetiva. O Tidiazurom é uma feniluréia com ação de citocinina que quando aplicado em baixas concentrações durante a divisão celular pode promover o aumento na frutificação efetiva e o tamanho dos frutos. O objetivo desse trabalho foi avaliar o efeito da concentração de tidiazurom na frutificação e qualidade de frutos de macieira ‘Royal Gala’, cultivadas em condições de inverno ameno. O experimento foi conduzido durante os ciclos 2011/2012, 2012/2013 e 2013/2014 em Fraiburgo, SC, em macieiras da cultivar Royal Gala em porta enxerto Marubakaido com filtro de M9. Em todos os ciclos de avaliação o delineamento experimental foi em blocos casualizados com esquema fatorial (6x3x2), com seis concentrações de TDZ, duas formas de aplicação e três anos de avaliação, composto por doze tratamentos com seis repetições, sendo a unidade experimental formada por uma planta. As concentrações de TDZ respectivas a cada tratamento foram aplicadas parceladamente em duas épocas. A primeira aplicação foi realizada no estádio de balão rosado (E2), e a segunda aplicação no estádio de plena floração (F2). Foram avaliadas as variáveis: frutificação efetiva (%), retorno de floração (%), número e massa de frutos por planta, massa fresca média de frutos (g), comprimento e diâmetro médio dos frutos e relação comprimento/diâmetro dos frutos (C/D), número médio de sementes, calibre por classes: ≤135; entre 136-165 e ≥180. Foi avaliado a coloração vermelha da epiderme dos frutos em três classes: porcentagem de frutos com coloração vermelha cobrindo menos de 50% da superfície do fruto; porcentagem de frutos com coloração vermelha cobrindo 50% a 80% da superfície do fruto; e porcentagem de frutos com coloração vermelha cobrindo mais de 80% da superfície do fruto. Foram determinados os teores minerais de N, P, K, Ca, Mg e suas relações da polpa fresca de frutos, firmeza de polpa, iodo-amido e conteúdo de sólidos solúveis totais (SST). Os dados obtidos foram submetidos à análise da variância e para as variáveis significativas foi realizada a análise de contrastes ortogonais. Os dados significativos também sofreram ajustes nas equações de regressão com a finalidade de avaliar o comportamento das variáveis com o aumento da concentração. Utilizou-se também a análise de correlação de Pearson para verificar possíveis relações existentes entre os diferentes atributos avaliados. O TDZ é efetivo, mesmo em baixas concentrações, no aumento da produção e frutificação efetiva de macieira sobretudo quando ocorrem problemas climáticos desfavoráveis à polinização. O TDZ retarda a maturação dos frutos. Há redução na porcentagem de coloração vermelha recobrindo a epiderme dos frutos e número de sementes por fruto nas plantas que receberam aplicação de TDZ. Os teores de minerais presentes na polpa mantem-se dentro da faixa de suficiência independente da concentração de TDZ aplicada

Made available in DSpace on 2016-12-06T17:42:05Z (GMT). No. of bitstreams: 1 PGPV14DA008.pdf: 1515332 bytes, checksum: 0e69f0adeb0a1a547c9cf883320c34c0 (MD5) Previous issue date: 2014-07-30 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The development of a breeding program for the pear culture has great importance to the expansion of the culture and the creation/selection of new rootstocks with traits of interest is able to impulse its productivity. Thus, main objective of this study was to select new genotypes from rootstocks seedlings of Pyrus communis L. providing plants of low vigor, and to develop a micropropagation protocol for these genotypes, aiming at sustainability of the pear culture in southern Brazil. The experiments were conducted at the Agroveterinaries Sciences Center at University of Santa Catarina State (CAV / UDESC). Plants used for the experiments were Pyrus communis L. with potential as rootstock for pear culture. The experimental design was completely randomized and the variables evaluated were: plant height, trunk diameter, bud number, branches of the year number and height, lenticels number (paper I) bacterial contamination, fungal contamination, explant survival (paper II) shoots number and height, bud number, leaf number, rooting percentage, root number and height, shoot height, callus intensity, explant survival (paper III) IAA production, rooting percentage, root number and height, shoot height and callus intensity (paper IV). Genotypes 409, 548, 570 and 577 remained constant in the group of medium vigor and were selected for micropropagation, in order to assess its potential in orchard conditions. At the in vitro establishment PPM is an effective biocide for controlling microbial contamination and in low concentrations do not affect survival of the explant. The asepsis with alcohol 70% + sodium hypochlorite 2,5% + PPM 2 mL L-1 in media culture is effective for explants derived from mother plants placed in a growth chamber and the asepsis with PPM 5% and PPM 5 % + PPM 2 mL L-1 in media culture are effective in explants derived from field mother plants. The QL modified medium plus 3,5 mL L-1 BAP showed higher multiplication of explants during the stage of in vitro multiplication. The QL modified medium with IBA in the range between 1,0 and 1,5 mL-1 showed better rooting during the rooting stage. The longest survival of explants was observed in the acclimatization using transparent cups with lid, containing the mix commercial substrate + vermiculite + coconut fiber (2:2:1). The explants of Pyrus communis L. should be acclimated 60 days after in vitro rooting having roots up to 30 mm. It was concluded that it was possible to select genotypes with superior characteristics within the population of Pyrus communis L. rootstocks, and it was possible to develop a micropropagation protocol for selections / O desenvolvimento de um programa de melhoramento para a cultura da pereira é de grande importância para a expansão da cultura e a criação/seleção de novos portaenxertos com características de interesse é capaz de impulsionar sua produtividade. Diante disto o objetivo principal deste trabalho foi selecionar novos genótipos de portaenxertos originários de seedlings de Pyrus communis L. que confiram menor vigor às plantas, e desenvolver um protocolo de micropropagação para estes genótipos, visando à sustentabilidade da cultura da pereira no sul do Brasil. Os experimentos foram conduzidos no Centro de Ciências Agroveterinárias da Universidade do Estado de Santa Catarina (CAV/UDESC). Foram utilizadas para os experimentos plantas da espécie Pyrus communis L. com potencial de portaenxerto para a cultura da pereira. O delineamento experimental utilizados foi inteiramente casualizado, as variáveis avaliadas foram: altura de planta, diâmetro do caule, número de gemas, número e comprimento de ramos do ano, número de lenticelas (artigo I) contaminação bacteriana, contaminação fúngica, sobrevivência de explantes (artigo II) número e comprimento de brotos, número de gemas, número de folhas, percentagem de enraizamento, número e comprimento de raiz, comprimento de parte aérea, intensidade de calo, sobrevivência de explantes (artigo III) produção de AIA, percentagem de enraizamento, número e comprimento de raiz, comprimento de parte aérea e intensidade de calo (artigo IV). Os genótipos 409, 548, 570 e 577 se mantiveram constantes no grupo de vigor médio e foram selecionados para a micropropagação, a fim de avaliar seu potencial em condições de pomar. No estabelecimento in vitro o PPM é um biocida eficaz no controle de contaminações microbianas e em baixas concentrações não afeta a sobrevivência do explante. A assepsia com álcool 70% + hipoclorito de sódio 2,5% + PPM 2 mL L-1 no meio de cultura é eficaz para explantes oriundos de plantas matrizes acondicionadas em câmara de crescimento e as assepsias PPM 5% e PPM 5% + PPM 2 mL L-1 no meio de cultura são eficazes para explantes oriundos de plantas do campo. O meio QL modificado acrescido de 3,5 mL L-1 de BAP apresentou maior multiplicação dos explantes durante o estádio de multiplicação in vitro. O meio QL modificado acrescido de AIB na faixa entre 1,0 e 1,5 mg L-1 apresentou melhor enraizamento in vitro durante o estádio de enraizamento. Maior sobrevivência foi verificada na aclimatização em copos transparentes com tampa, contendo a mistura substrato comercial + vermiculita + fibra de coco (2:2:1). Os explantes de Pyrus communis L. devem ser aclimatizados 60 dias após o enraizamento in vitro, possuindo raízes com até 30 mm. Conclui-se que foi possível selecionar genótipos com características superiores dentro da população de portaenxerto de Pyrus communis L., bem como, foi possível desenvolver um protocolo de micropropagação para as seleções

Resumo: Face à carência de estudos relacionados à fisiologia pós-colheita de flores, a presente pesquisa teve como finalidade estudar a fisiologia e conservação póscolheita de flores cortadas de rosas cv. Vega, considerando os fatores associados à senescência floral e perda de vida decorativa. Os experimentos foram conduzidos em delineamento inteiramente casualizado, em esquema fatorial. Em todos os experimentos, as rosas foram mantidas em ambiente de laboratório com 20±2ºC e 67±3% UR, padronizadas em 50 cm e realizadas as seguintes avaliações (exceto para o experimento 5): perda de massa fresca, massa seca, taxa respiratória, notas de qualidade (escurecimento, turgescência e curvatura), conteúdo relativo de água, carboidratos solúveis e redutores, antocianina e coloração. Para o experimento 5 foi avaliada a quantidade de água absorvida e transpirada das rosas. No primeiro experimento, as flores foram colocadas dentro de uma câmara hermética e expostas ao 1-MCP, por um período de seis horas, nas seguintes concentrações: 1) Água destilada (controle); 2) 1-MCP 100 a ppb; 3) 1-MCP a 250 ppb; 4) 1-MCP a 500 ppb. Ao contrário do tratamento com água destilada, todas as concentrações de 1-MCP foram eficientes para retardar a senescência das flores, com destaque para a de 500 ppb que melhor manteve a qualidade, além de prolongar a vida de vaso das rosas até 19 dias. No segundo experimento, as flores permaneceram nas seguintes soluções de manutenção: 1) Água destilada (Controle); 2) 8-HQC (200 mg.L-1); 3) Sacarose (2%) + 8-HQC (200 mg.L-1); 4) Ácido Cítrico (75 mg.L-1); 5) Sacarose (2%) + Ácido Cítrico (75 mg.L-1); 6) 6- BA (60 mg.L-1); 7) Sacarose (2%) + 6-BA (60 mg.L-1). As rosas foram muito sensíveis à 6-benziladenina, associada ou não à sacarose, e perderam a qualidade aos seis dias de vida de vaso. Em contrapartida, o tratamento com 8-hidroxiquinolina ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Given the lack of studies on postharvest physiology of flowers, this research aims to study the postharvest physiology and keeping quality of Vega cut rose, were observed the factors associated with floral senescence and loss of decorative life. The experiments followed complete randomized design, in factorial arrangement. In all experiments, the roses were kept at room temperature with 20 ± 2ºC and 67±3% UR, standardized at 50 cm and it were analysis (except the fifth experiment): weight loss, dry weight, rate respiratory, quality (browning, turgidity and curvature), relative water content, soluble and reducing carbohydrates, anthocyanin, color and longevity. On the fifth experiment was to evaluation the water absorbed and transpired roses. On the first experiment, the flowers were placed inside an airtight chamber and exposed to 1-MCP for a period of six hours, in these concentrations: 1) Distilled water (control); 2) 1-MCP (100 ppb); 3) 1-MCP ( 250 ppb); 4) 1-MCP (500 ppb). Unlike treatment with distilled water, all concentrations of 1-MCP were effective in delaying the senescence of flowers, however, the concentration of 500 ppb of 1-MCP induced better maintenance of quality and extended vase life of roses to 19 days. On the second experiment, the flowers remained in these following holding solutions: 1) Distilled water (Control); 2) 8-HQC (200 mg.L-1); 3) Sucrose (2%) + 8-HQC (200 mg.L-1); 4) Citric Acid (75 mg.L-1); 5) Sucrose (2%) + Citric Acid (75 mg.L-1); 6) 6-BA (60 mg.L-1); 7) Sucrose (2%) + 6-BA (60 mg.L-1). The roses were very sensitive to 6-benzyladenine, with or without sucrose, and lost quality of six days of vase life. In contrast, treatment with 8-hydroxyquinoline has proved the most promising to maintain the quality of the flowers, for ten days. On the third experiment, the roses were treated this way: 1) Distilled water (Control), 2) STS (1 mM) ... (Complete abstract click electronic access below) / Orientador: Ben-Hur Mattiuz / Coorientador: Teresinha de Jesus Deléo Rodrigues / Coorientador: Claudia Fabrino Machado Mattiuz / Banca: Kathia Fernandes Lopes Pivetta / Banca: Regina Maria Monteiro de Castilho / Mestre

Orientador: Orivaldo Arf / Banca: Antonio Cesar Bolonhezi / Banca: Rita de Cassia Felix Alvarez / Resumo: O acamamento de alguns cultivares de arroz no momento da colheita acarreta perdas significativas na produtividade, além disso, os manejos de água e nitrogênio inadequados em condições de alta tecnologia agravam mais o problema. O uso de reguladores vegetais é uma das alternativas para reduzir o acamamento, entretanto, as informações sobre o assunto são escassas. O trabalho foi desenvolvido com o objetivo de avaliar o uso de doses de etiltrinexapac (zero; 75, 150, 225 e 300 g ha-1 do i.a.) e épocas de aplicação (perfilhamento ativo, entre o perfilhamento ativo e a diferenciação floral e na diferenciação floral) no desenvolvimento e produtividade da cultura do arroz de terras altas. O experimento foi desenvolvido no município de Selvíria - MS, durante o ano agrícola de 2006/07. Concluiu-se que a aplicação de 150 g ha-1 de etil-trinexapac no momento da diferenciação floral do arroz cultivar Primavera reduz a altura de plantas, na média 0,40 m em relação às outras duas épocas, com ausência de acamamento; o etil-trinexapac promove maior número de grãos chochos, menor perfilhamento útil, reduzindo a produtividade de grãos em doses acima de 150 g ha-1, quando aplicado no estádio de diferenciação floral e, a aplicação de 75 e 150 g ha-1 de etil-trinexapac em qualquer época não interfere na produtividade de grãos. / Abstract: The lodging of some rice cultivars at harvest time leads to significant yield losses and, besides this, the inadequate water and nitrogen management when high technology is used may aggravate this problem. The use of plant growth regulators is one of the alternatives for reducing the lodging; however, the information on such subject is not enough. The objective of this experiment was to evaluate the effect of ethyl-trinexapac doses (zero, 75, 150, 225 and 300 g ha-1 of the a.i.) and the application periods (at active tillering, between active tillering and floral differentiation and at floral differentiation) on the crops development and yield in non-flooded lands. The experiment was carried out in Selvíria - MS, during the agricultural year of 2006/07. The conclusion was that the application of 150 g ha-1 of ethyl-trinexapac at floral differentiation of the Primavera cultivar reduces the plants' height, average of 0.40 m when compared to the two other application periods, and leads to lack of lodging; ethyltrinexapac leads to a higher number of dry grains and less useful tillering, reducing the grain yield when the doses are higher than 150 g ha-1 and applied at floral differentiation ; the application of 75 and 150 g ha-1 of ethyl-trinexapac at any of the stages does not influence the grain yield. / Mestre

Submitted by Deyse Queiroz (deysequeirozz@hotmail.com) on 2018-05-23T13:54:28Z No. of bitstreams: 1 JOYCE EMANUELE DE MEDEIROS - DISSERTAÇÃO PPGSA PROFISSIONAL 2013..pdf: 1718564 bytes, checksum: 08bf95bc06f72832fe2269039eb98219 (MD5) / Made available in DSpace on 2018-05-23T13:54:28Z (GMT). No. of bitstreams: 1 JOYCE EMANUELE DE MEDEIROS - DISSERTAÇÃO PPGSA PROFISSIONAL 2013..pdf: 1718564 bytes, checksum: 08bf95bc06f72832fe2269039eb98219 (MD5) Previous issue date: 2013 / A partenocarpia em melancia agrega valor aos frutos, entretanto, para seu cultivo, o produtor poderá encontrar dificuldades, como: adaptação dos híbridos às condições regionais, alto preço das sementes, baixa germinação e baixo vigor das plântulas, além da necessidade de cultivar na mesma área uma variedade diploide como fonte de pólen para as triploides. Assim, objetivou-se avaliar o efeito da aplicação da citocinina CPPU, em diferentes concentrações, na indução da frutificação partenocárpica em melancieiras diploide e triploide. A pesquisa foi constituída por dois experimentos conduzidos em campo no Câmpus da UFCG, em PombalPB, no período de 15/12/2012 a 02/03/2013 utilizando-se o híbrido triploide ‘Extasy’ e a variedade diploide ‘Crimson Sweet’. Para ambos os experimentos, os tratamentos foram compostos por cinco concentrações do regulador de crescimento CPPU (0,75; 1,0; 1,5; 2,5 e 3,5 mg L-1 ) mais testemunha (polinização natural). O delineamento experimental utilizado foi o de blocos casualizados, com quatro repetições. A aplicação do CPPU ocorreu de forma exógena no ovário das flores na antese por dois dias consecutivos. As características avaliadas foram: taxa fotossintética (A), condutância estomática (gs), transpiração (E), concentração intercelular de CO2 (Ci), eficiência no uso da água (EUA), clorofilas, carotenoides, número de folhas, área foliar, massa seca das folhas, do caule e dos frutos e massa seca total, produção total e comercial, massa média dos frutos comercializáveis, número de frutos por planta, número de frutos comercializáveis por planta, índice de formato do fruto, espessura da casca e da polpa, sólidos solúveis, acidez titulável, pH, SS/AT e ácido ascórbico. O CPPU foi eficiente na indução da frutificação em melancieiras diploide ‘Crimson Sweet’ e triploide ‘Extasy’, independentemente da concentração utilizada. A concentração 2,5 mg L-1 de CPPU aplicada nas melancieiras diploides proporcionou o maior número de frutos comercializáveis por planta, maior produção total e produção comercial comparada à testemunha, já nas triploides possibilitou maior número de frutos por planta, número de frutos comercializáveis por planta, produção total e produção comercial em relação à testemunha. A indução da frutificação com CPPU na melancieira ‘Crimson Sweet’ não interferiu no acúmulo de massa seca, no crescimento e nas características fisiológicas da planta comparada à testemunha. Na ‘Extasy’, proporcionou maior acúmulo de massa seca no fruto, redução na massa seca das folhas, do caule e crescimento da planta, incremento na condutância estomática e na assimilação de CO2 quando comparada à testemunha. Nas melancieiras ‘Crimson Sweet’, a concentração de CPPU 2,5 mg L-1 proporcionou maior condutância estomática, taxa fotossintética e acúmulo de massa seca no fruto. Na melancieira ‘Extasy’, as concentrações de CPPU foram indiferente nas trocas gasosas e no crescimento da planta. / Parthenocarpy in watermelon adds value to fruits, however, for its cultivation, the producer may encounter difficulties such as: adaptation of hybrid to regional conditions, high seed prices, low germination and seedling vigor, beyond the need to cultivate, in the same area, a diploid variety as source of pollen for triploids. Thus, the objective was to evaluate the effect of application of cytokinin CPPU at different concentrations, in the induction of fruiting partenocarpic in diploid and triploid watermelon plants. The study comprised two field experiments conducted in the field in Campus of UFCG in Pombal -PB, from 12/15/2012 to 03/02/2013 using the triploid hybrid 'Extasy' and diploid variety 'Crimson Sweet'. For both experiments, treatments consisted of five concentrations of growth regulator CPPU (0.75, 1.0, 1.5, 2.5 and 3.5 mg L- 1 ) and the control (natural pollination). The experimental design was a complete randomized block designed with four replications. The application of CPPU occurred exogenously in the ovary of flowers for two consecutive days at anthesis. The variables evaluated were: photosynthetic rate (A), stomatal conductance (gs), transpiration (E), intercellular CO2 concentration (Ci), water use efficiency (EUA), chlorophylls, carotenoids, number of leaves, leaf area, dry mass of leaves, stem and fruit and total dry matter, total and marketable yield, average weight of marketable fruit , number of fruits per plant, number of marketable fruits per plant, fruit shape index, shell thickness and pulp, soluble solids, titratable acidity, pH, SS/TA and ascorbic acid. The CPPU was efficient in inducing fruiting of diploid watermelon 'Crimson Sweet' and triploid 'Extasy’, regardless of the concentration used. The concentration 2.5 mg L- 1 of CPPU applied in diploid watermelon provided the greatest number of marketable fruits per plant, higher total and marketable yield production compared to control, and in the triploid plants in increased the number of fruits per plant, number of fruits marketable per plant, total production and commercial production compared to control. Induction of fruiting with CPPU in watermelon ‘Crimson Sweet’ had no effect on dry matter accumulation, growth and physiological characteristics of the plant compared to the control. In 'Extasy', CPPU provided a greater accumulation of dry matter in the fruit decreased dry mass of leaves, stem and plant growth, increase in stomatal conductance and CO2 assimilation compared to the control. In watermelon 'Crimson Sweet' the concentration of 2.5 mg L- 1 yielded higher stomatal conductance, photosynthetic rate and dry matter accumulation in the fruit. In watermelon 'Extasy', concentrations of CPPU were indifferent gas exchange and plant growth.