Cette thèse porte sur la production des espaces domestiques et villageois en milieu rural et s’appuie sur une ethnographie menée pendant plus de dix-huit mois dans le village malinké de Guirlan, situé au nord-est de la Guinée. Elle articule les notions d’espace, de mobilité et de relation sociale en insistant sur les pratiques quotidiennes des habitants. L’objet privilégié est celui de la résidence (lu), dont l’organisation topocentrique est analysée comme déterminante dans la construction des relations sociales. La démarche se veut elle aussi spatialisée. Le raisonnement débute dans les cours centrales, là où l’autorité est mise en acte. Il se poursuit dans la périphérie des espaces domestiques, puis en dehors du village. Les mines d’or, résultat de l’intensification de l’exploitation aurifère depuis les années 1980-1990 dans la région, sont alors présentées comme des espaces relativement autonomes comportant leurs propres règles. En revanche, les installations plus durables de migrants dans des villes plus lointaines, sont considérées comme des extensions de la résidence d’origine, permettant les mobilités de certains de ses membres. L’enjeu est en fait celui de la présence et des temporalités de l’absence. Alors que certains déplacements de courte distance sont analysés comme des ruptures, comme l’accompagnement de l’épouse chez son mari, d’autres, plus lointains, reposent sur une absence pensée dans sa présence future. À travers l’étude des pratiques spatiales, ce travail interroge donc plus largement la question de la reconnaissance sociale et de sa mise en visibilité au quotidien. / This thesis on the production of domestic and village spaces in a rural setting, is based on more than eighteen months of ethnographic fieldwork in the Malinke village of Guirlan in northeastern Guinea. It explores the interplay of space, mobility and social relationship, notably in the course of the inhabitants’ daily practices. The central object of this study is the residence (lu), whose topocentric organization is held to be determinate in the construction of social relations. The analysis itself also spatially grounded. Beginning in the central courtyards where authority is enacted, it moves out to the periphery of domestic areas, and then beyond the village itself. Gold mines, resulting from an intensification of mining in the region since 1980-1990, are seen as relatively autonomous spaces governed by rules of their own. On the other hand, more prolonged installations of migrants in cities further away are envisaged as extensions of their home residences that allow for the mobility of some of their members. All these cases deal with the issues of presence and the temporalities of absence. While certain short-range displacements, such as the accompaniment of a wife to her husband’s residence, are analyzed as ruptures or breaches, other more distant ones consist in an absence conceived as presence-to-come. By focusing on spatial practices, this study is directly concerned with the problem of social acknowledgement and its visibilization in daily life.

Orientador: Carlos Augusto Araújo Valadão / Banca: Juan Carlos Duque Moreno / Banca: Newton Nunes / Banca: Ricardo Miyasaka de Almeida / Banca: Valéria Nobre Leal de Souza Oliva / Resumo: Objetivou-se, com este estudo, determinar se o butorfanol modifica a concentração anestésica mínima do sevofluorano (CAnMSEV) em função do tempo e avaliar os efeitos cardiorrespiratórios e eletrolíticos causados por essa associação em galinhas d'angola. Dez galinhas d'angola foram anestesiadas com sevofluorano e mantidas sob ventilação mecânica para determinação da CAnMSEV individual pelo modelo bracketing. A porcentagem de redução da CAnMSEV após a administração intravenosa de 2 ou 4,0 mg/kg de butorfanol em função do tempo foi estimada por regressão logística pelo método "up-and-down". Em etapa subsequente, foram avaliados os efeitos cardiorrespiratórios do sevofluorano (1,0 CAnM) e de uma dose equipotente do sevofluorano (0,8 CAnM) associado ao butorfanol (4,0 mg/kg). A CAnMSEV média foi de 2,86 ± 0,09V%. Após 15 minutos da administração de 2,0 mg/kg de butorfanol, a redução da CAnMSEV foi de 8,52 ± 3,11V%. Após 15 e 30 minutos da administração de 4,0 mg/kg de butorfanol, a redução da CAnMSEV foi de 20,78 ± 4,37V% e 11,07 ± 8,2V%, respectivamente. A administração intravenosa de 4,0 mg/kg de butorfanol induziu taquicardia supraventricular e fibrilação ventricular em 62,5% e 25% dos animais, respectivamente. Foi observada diminuição da frequência cardíaca, hipotensão e depressão respiratória severa 1 minuto após a administração de 4,0 mg/kg de butorfanol. O butorfanol diminui de forma dosedependente a CAnMSEV por um curto período, contudo, a dose que proporcionou a maior redução da CAnMSEV não foi considerada segura nas condições deste estudo / Abstract: The aim of this study was to determine the sevoflurane minimum anesthetic concentration (MACSEV), to measure the dose and temporal sevoflurane MAC sparing effect of butorphanol, and to evaluate the cardiorrespiratory changes of this combination in guinea fowl. Ten guinea fowls were anesthetized with sevoflurane under mechanical ventilation for individual MACSEV determination using the bracketing design. The percent of MACSEV reduction after intravenous administration of 2 or 4,0 mg/kg of butorphanol was estimated by logistic regression using the up-and-down method. Afterward, the cardiorespiratory changes of sevoflurane (1.0 MAC) and of an equipotent dose of sevoflurane (0.8 MAC) combined with butorphanol (4.0 mg/kg) were studied. The mean MACSEV was 2.86 ± 0.09%. After 15 minutes of the 2-mg/kg butorphanol injections, the sevoflurane MAC reduction was 8.52 ± 3.11%. After 15 and 30 minutes of the 4-mg/kg butorphanol injections, the sevoflurane MAC reduction was 20.78 ± 4.37% and 11.07 ± 8.2%, respectively. Intravenous administration of 4.0 mg/kg of butorphanol caused supraventricular tachycardia and ventricular fibrillation in 62.5% and 25% of the animals, respectively. Decrease in heart rate, severe hypotension, and respiratory depression were observed after one minute of butorphanol administration. Butorphanol dose-dependently decreased MACSEV for a short period; however, the dose that caused MAC sparing effect was not considered safe in this experimental conditions / Doutor

Les ondes internes sont observées partout dans le monde. Elles ont un rôle important dans la mise en place de la chaine trophique, et elles peuvent avoir un impact sur les activités humaines. Ainsi, dans le Golfe de Guinée, des ondes internes solitaires (OIS) ont provoqué des incidents sur des plateformes pétrolières. L’objectif de la thèse est d’identifier leurs zones de génération, leurs directions de propagation, ainsi que les conditions d’environnement susceptibles de les déclencher. La thèse a comporté deux aspects : d’une part des mesures de courant étaient disponibles et d’autre part la mise en place de modèles numériques. Ces mesures ont montré le passage d’OIS. En outre, les zones de génération potentielles sont identifiées en haut du talus continental. Leur répartition mensuelle a montré une variabilité saisonnière, due à la Mousson Ouest Africaine qui modifie les conditions de stratification au cours de l’année.La modélisation a été réalisée avec HYCOM. Une maquette hydrostatique régionale du Golfe de Guinée a été mise en place. Elle a d’abord été validée en configuration barotrope (océan homogène). Une configuration bicouche a ensuite été testée, et la théorie linéaire des ondes internes a été vérifiée sur ses résultats. Des termes non hydrostatiques ont ensuite été ajoutés dans les équations horizontales du courant de HYCOM, pour modéliser des OIS. L’impact des paramètres physiques, en particulier la force de Coriolis et le forçage barotrope, ainsi que l’effet d’un courant moyen, a été étudié sur des configurations bicouches radiales. Enfin, un canyon a été ajouté au milieu du talus pour évaluer qualitativement les effets d’une bathymétrie 2D sur les OIS. / Internal waves are observed in different locations. They have a key role in the set up of the trophic chain, and they can impact human activities.For instance, in the Gulf of Guinea, internal solitary waves (ISWs) have caused hazards on offshore oil platforms. The aim of the thesis is to identify the generation zones and the directions of propagation of these ISWs, and the environmental conditions (tides, stratification) that can generate them. One the one hand measurements of currents were available, and on the other hand numerical models were used.Measurements of currents were analyzed. Packets of ISWs were identified. Moreover, the potential generation zones of the ISWs, particularly the top of the shelf break, were inferred from their direction of propagation. The monthly repartition of the packets of ISWs revealed a seasonal variability, due to the West African Monsoon, which modified the conditions of the stratification throughout the year.Concerning the modelling, the numerical model HYCOM was used. One regional hydrostatic model of the Gulf of Guinea was built. This regional model was validated for a barotropic configuration (homogeneous ocean) first. Then, a two-layered configuration was set up, and the linear theory of the internal waves was confirmed on the results.Non hydrostatic terms were added to the horizontal velocity equations in HYCOM, to model ISWs. The influence of different physical parameters, such as the Coriolis force, the barotropic forcing, and a mean current was studied on two-layered radial configurations. Finally, a canyon in the middle of the shelf break was set up to assess qualitatively the effect of a 2D bathymetry on the ISWs.

Na última década, o uso de sistemas microventilados (sistema VMA) em biotérios possibilitou alojar animais com melhor qualidade sanitária devido ao maior controle ambiental fornecido por esses sistemas. Contudo, ainda é necessária uma melhor avaliação, principalmente no que tange às respostas dos animais. Dessa forma, o objetivo deste trabalho foi avaliar a integridade do aparelho respiratório (pulmões e traquéia) de cobaias mantidas sob dois sistemas de ventilação diferentes: ventilação geral diluídora (VGD) ou ventilação para conforto e ventilação microambiental (VMA). Foram utilizados quatro grupos de animais que ficaram alojados por 180 dias no CREAL-UFRGS. Os animais foram acompanhados com exames radiológicos dos pulmões a cada 45 dias. A histopatologia foi realizada no último dia de acompanhamento dos animais.Os resultados da análise radiológica não demonstraram diferença significativa entre as lesões pulmonares de cobaias fêmeas e machos mantidas sob os dois sistemas de ventilação. A avaliação histopatológica evidenciou lesões descamativas e de metaplasia no epitélio da traquéia de animais de ambos os sexos e sistemas de ventilação. As lesões encontradas nos pulmões das cobaias machos e fêmeas de ambos os sistemas foram em sua maioria lesões inflamatórias, com intenso infiltrado de mononucleares. A presença e intensidade dessas lesões variaram de acordo com o sistema de ventilação sendo que no grupo VGD é que foram encontradas lesões mais severas. / During the last decade, the use of intracage ventilation systems (VMA) in animal facilities has improved the health status of animal by better environmental control supplied by these systems. Yet, here is little information upon use of VMA systems, mainly in that it referes to animal responses. Thus, the purpose in this investigation was to evaluate epithelial integrity in airways (trachea and lungs) of Guinea pigs kept in two different systems: general diluting ventilation system or comfort ventilation system (VGD) and intracage ventilation systems (VMA). Four groups of animals were kept for 180 days on CREAL- UFRGS. The animals were observed with radiological exam taked from lungs every 45 days. The histophatology was done in the last day of trial. The results show the radiological exams haven’t differences between lung injuries from males and females kept in two different ventilation systems. The histological examination showed lost and changes of trachea epithelium in both, male and female under two different ventilation. The injuries found in lung from Guinea pigs, male and female, under two different ventilation were in the most inflammatory injuries, predominantly mononuclear cells. Intensity of lesions varied according to the ventilation system wich animals were kept, the animals kept in VGD system have presented more intensity of injuries.

The aim of this thesis is to analyse EU response towards the Ebola outbreak that developed in spring and summer 2014 in Western Africa. The emphasis is put on the EU external action towards the Ebola crisis in the frame-work of the EU development policy and humanitarian aid provided by the EU while identifying the financial framework, institutional structure, key players and the role of the EU Members. It was a common endeavour of whole range of global actors, national governments and many regional or local stakeholders that led to elimination of the threat represented by the Ebola Virus Disease. The European Union traditionally empha-sized multilateral approach that is in compliance with the EU middle power capacities. The de-velopment policy financial mechanisms within the EU have two resources, the EC and the Mem-ber States which cumulatively contributed twice as much as the European Commission to re-solving the crisis.

The aim of the work was to find out and analyse social interactions and activities of the guinea pigs (Cavia aperea var. porcellus) during 24 hours. Five groups of short-haired guinea pigs were observed. The guinea pigs were filmed. Next, video records were analysed using ethograms and description of social behaviour between group members. Data record was written down to ethograms every 1 minute for a periof of 24 hours. Observed activities were food intake, resting (including sleep), movement and comfort beahaviour (grooming). Generally, eighteen guinea pigs (in five groups) were observed. Guinea pigs are friendly animals, they create stable and well-organised groups. None aggressive behaviour was observed between group members. Besides small conflicts because of age difference, food or interruption during sleep. The oldest sows were dominant and their place was close to food. It was found out that guinea pigs rested the most (726 minutes, half-day). Food intake made up 590 minutes (41 %) and movement 84 minutes (6 %). More active were younger guinea pigs and boar who was housed with two sows. Comfort behaviour took 41 minutes, guinea pigs also took cares of each other.

O objetivo da pesquisa foi de analisar a alternativa de Produção de Biodiesel na Guiné-Bissau, tendo como base a transposição do aprendizado construído pelo Programa de Produção e Uso do Biodiesel no Brasil – PNPB, analisar a realidade do PNPB no Brasil, verificar a percepção de viabilidade junto aos agentes do governo guineense, associação de classe de agricultores e construir um modelo preliminar do Quadro Institucional do Programa de Biodiesel na Guiné, como alternativa para promover ascensão econômica e desenvolvimento regional. O êxito do PNPB foi evidenciado pelos benefícios proporcionados na economia brasileira. Até 2010 o Brasil já deixou de gastar U$ 2,84 bilhões com importação de diesel. Com a adição de B10, o Brasil não precisaria mais importar diesel. Para ampliar a produção do biodiesel até 2020, projeta-se investimento de R$ 7,36 bilhões, o que geraria 531.045 empregos diretos e 6 milhões indiretos. A Guiné-Bissau enfrenta problema da pobreza extrema e economia baseada na monocultura de caju. Os gastos com combustível fóssil em 2015 representaram 30% de total das importações do país, contribuindo fortemente para o déficit na balança comercial do país e no enfraquecimento da economia já fragilizada pela pobreza. A escassez da energia impulsionou as empresas locais a utilizarem geradores movidos a óleo diesel para suprir esta demanda. Apesar da evidência dos esforços governamentais no fornecimento da energia, o preço praticado não tem a mesma proporção com a condição econômica do país. Neste sentido, o Programa de Biodiesel na Guiné-Bissau – PNBGB se caracteriza num mecanismo proeminente para alavancar a economia daquele país, por ser uma atividade com elo na agricultura e com potencial de geração de emprego e renda no campo e redução das emissões de gases de efeito estufa – GEE na atmosfera. Para alcançar os objetivos da pesquisa foi utilizada a metodologia de pesquisa exploratória. Os dados foram coletados em Bissau, Guiné-Bissau, inicialmente por entrevistas pelo Skype e finalizados com aplicação de questionários por e-mail. As entrevistas contaram com a participação do Ministro da Economia e Finanças e pelo Ministério da Agricultura e Desenvolvimento Rural da Guiné-Bissau, por um conjunto de técnicos e especialistas da área. Os resultados demonstraram que a Guiné-Bissau apresenta grande potencial de produção de biodiesel a partir de oleaginosas existentes no país como jatropha curcas, palma de dendê, algodão e mamona. No entanto, a planta indicada pelo Ministério da Agricultura é a jatropha. Com isso, foi realizada uma projeção da demanda e oferta de biodiesel até 2031, o que permitiu verificar que a escala viável economicamente para Guiné-Bissau inicia com o nível de mistura compulsória de 5% – B5, equivalente ao volume de 4.091,14 m³/ano de biodiesel. Verificou-se que os benefícios do Programa de Biodiesel na Guiné-Bissau poderão envolver as receitas fiscais, geração de emprego e renda para mais de 80.000 pessoas que atuam no setor agrícola, benefícios ambientais e econômicos na diversificação da base produtiva, além de impulsionar a criação das cooperativas agrícolas com notável atuação na cadeia produtiva do biodiesel. A partir desta análise, foi elaborada uma proposta de Quadro Institucional e de implicações de gestão para implantação do PNBGB. A análise da implantação do programa apresentou a necessidade de transferência tecnológica e de financiamentos estrangeiros. Neste contexto, observou-se que o país conta com parceiros sub-regionais e internacionais para financiamento de projetos de desenvolvimento e de transferências de tecnologias agrícolas, elevando o potencial de sucesso na introdução do Programa de Biodiesel na Matriz Energética guineense. / The objective of this study was to examine the alternative of Biodiesel Production in Guinea- Bissau, taking as a basis the implementation of the learning constructed by the Program for the Production and Use of Biodiesel in Brazil - PNPB, examine the reality of PNPB in Brazil, check the perception of viability by the agents of the Guinean government, association of farmers and build a model of the Institutional Framework of the Biodiesel Program in Guinea, as an alternative to promote economic advancement and regional development. The success of the PNPB was evidenced by the benefits provided in the Brazilian economy. Until 2010 Brazil has already failed to spend $2.84 billion with imports of diesel. With the addition of B10, Brazil does not need more importing diesel. To increase the production of biodiesel until 2020, the projected investment of R$ 7.36 billion, which would generate 531,045 direct jobs and 6 million indirect jobs. The Guinea-Bissau faces problem of extreme poverty and economy based on monoculture plantations of cashew nuts. Spending on fossil fuel subsidies in 2015 accounted for 30% of total imports of the country, contributing greatly to the deficit in the trade balance of the country and the weakening of the economy already weakened by poverty. The shortage of energy has boosted the local companies to use generators powered by diesel oil to meet this demand. Despite the evidence of governmental efforts in the provision of energy, the price does not have the same proportion with the economic condition of the country. In this sense, the Biodiesel Program in Guinea Bissau - PNBGB is characterized in a prominent mechanism to leverage the economy of that country, because it is an activity with a link at the agriculture and with potential for generating employment and income in the country and reduction of greenhouse gas emissions - GHG in the atmosphere. To achieve the objectives of the survey was used the methodology of explo ratory research. The data were collected in Bissau, Guinea-Bissau, initially by interviews via Skype and finalized by application of questionnaires by mail. The interviews were attended by the Minister of Economy and Finance and the Ministry of Agriculture and Rural Development of Guinea-Bissau, for a set of technicians and specialists in the area. The results showed that the Guinea-Bissau presents great potential for production of biodiesel from oilseeds in the country as jatropha curcas, palm oil, cotton and castor oil. However, the plant indicated by the Ministry of Agriculture is the jatropha. With this, there was a projection of the demand and supply of biodiesel until 2031, which showed that the scale economically viable for Guinea- Bissau starts with the level of compulsory mixture of 5% - B5, equivalent to the amount of 4,091.14 m³/year of biodiesel. It was found that the benefits of the Biodiesel Program in Guinea Bissau may involve the tax revenue, generation of employment and income for more than 80,000 people working in the agricultural sector, environmental benefits and economic diversification of the productive base, in addition to boost the creation of agricultural cooperatives with remarkable performance in the biodiesel production chain. From this analysis, a proposal was drawn up to the institutional framework and implications for management for deployment of PNBGB. The analysis of the implementation of the program presented the need for technology transfer and foreign financing. In this context , it was noted that the country has partners sub-regional and international financing of development projects and for transfers of agricultural technologies, increasing the potential for success in the introduction of the Biodiesel Program in the energy matrix.

O estudo chama a atenção para o fenômeno do analfabetismo na população jovem e adulta da Guiné-Bissau, buscando compreender por que a alfabetização de jovens e adultos ainda não figura entre as prioridades da política educacional desse país africano, identificando as possibilidades de transformação dessa realidade e os fatores que interferem no processo educacional. Tendo por base a prática pedagógica de Paulo Freire, que considera o desenvolvimento do indivíduo como resultado do processo histórico, o estudo enfatiza que aspectos relativos à diversidade cultural, marcadamente as variedades linguísticas e religiosas presentes na Guiné-Bissau, devem ser considerados. A diversidade cultural, as cosmologias próprias vinculadas a diferentes idiomas e religiosidades compõem uma “África Profunda”, conforme a obra do filósofo argentino Rodolfo Kusch. Foi realizado levantamento bibliográfico e documental sobre a Alfabetização de Jovens e Adultos na Guiné-Bissau, sendo localizadas algumas dissertações de mestrado e teses de doutorado na perspectiva do estudo da questão. A pesquisa está estruturada também em torno dos referenciais da pesquisa qualitativa em Educação, e, a partir dessa concepção, foi feita a interpretação dos dados obtidos por meio das entrevistas junto aos facilitadores, técnicos e à diretora de Alfabetização de Adultos do Ministério da Educação da Guiné-Bissau, bem como a estudantes jovens e adultos que frequentam turmas de alfabetização em nove comunidades visitadas durante o trabalho de campo, desenvolvidos de maio de 2017 a fevereiro de 2018 O método de alfabetização ALPHA TV é desenvolvido nas comunidades visitadas, sendo utilizado pela maioria dos facilitadores. A observação participante contribuiu para a organização e construção de diários de campo, nos quais se descrevem os espaços e processos educativos que aí ocorrem. Foi realizado também um mapeamento das experiências públicas e privadas de alfabetização de jovens e adultos na Guiné-Bissau, descrevendo as condições sociais e políticas, e o sistema educacional vigente no país. O estudo conclui que o estado guineense precisa implementar um sistema educacional para a alfabetização de jovens e adultos, enquanto política pública destinada a todo o seu território. Para isso, precisa contar com a participação da sociedade e valorizar ou considerar a sua variedade linguística e religiosa, a fim de promover o desenvolvimento social do país. / The study draws attention to the phenomenon of illiteracy in the young and adult population of Guinea-Bissau, trying to understand why the literacy of young people and adults is not yet among the priorities of the educational policy of this African country, identifying the possibilities of transformation of this reality and factors that interfere with the educational process. Based on the pedagogical practice of Paulo Freire, who considers the development of the individual as a result of the historical process, the study emphasizes that aspects related to cultural diversity, markedly the linguistic and religious varieties present in Guinea-Bissau, should be considered. Cultural diversity, its own cosmologies linked to different languages and religiosities make up a "Deep Africa", according to the work of the Argentine philosopher Rodolfo Kusch. A bibliographical and documentary survey was carried out on the Literacy of Youths and Adults in Guinea-Bissau, where some master's dissertations and doctoral theses were located in view of the study of the issue The research is also structured around the qualitative research in Education, and from this conception, the data obtained through the interviews with the facilitators, technicians and the Director of Adult Literacy of the Ministry of Education of the Guinea- Bissau, as well as young students and adults attending literacy classes in nine communities visited during field work, from May 2017 to February 2018. The ALPHA TV literacy method is developed in the communities visited and is used most of the facilitators. Participant observation contributed to the organization and construction of field diaries, in which the educational spaces and processes that occur there are described. A mapping of public and private experiences of youth and adult literacy in Guinea-Bissau was also carried out, describing the social and political conditions and the educational system in force in the country. The study concludes that the Guinean state needs to implement an educational system for youth and adult literacy as a public policy aimed at its entire territory. To do this, it needs to count on the participation of society and value or consider its linguistic and religious variety, in order to promote the social development of the country.

This study aimed to evaluate the ability of nymphs of Amblyomma ovale naturally infected with Rickettsia parkeri (Atlantic Forrest strain) in transmitting it to Cavia porcellus (guinea pigs), and analyze the infection in these animals. A total of 26 guinea pigs divided into three groups were used: G1 - 10 guinea pigs infested with nymphs of uninfected A. ovale; G2 - 10 guinea pigs infested with nymphs of A. ovale naturally infected with R. parkeri (Atlantic Forrest strain) and G3 - 6 uninfected guinea pigs. A tick infestation chamber was fixated on the animals, where 25 nymphs of A. ovale, either infected or not, were placed. In the first study, the vector competence of A. ovale nymphs in the transmission of R. parkeri (Atlantic Forrest strain) for C. porcellus (animals of G1 and G2) was evaluated. After the period of parasitism, engorged nymphs were collected and stored in a B.O.D incubator. To assess the anti-Rickettsia spp antibodies, blood was collected at 7, 14, 21 and 28 days post infestation (DPI) being evaluated by indirect immunofluorescence assay (IFA). To identify the multiplication of Rickettsia in the tissue from guinea pigs a polymerase chain reaction was carried out at 7, 10, 14 and 28 DPI. To verify the vector competence of nymphs, parasite periods, the percentage of molting, transstadial survival and IFA were analyzed. The average period of parasitism in G1 was 6.6 days and 6 days in G2. The average percentage of molting (nymph to adult) was 95%. In G2, the survival of transstadial Rickettsia was confirmed in 100% (PCR) and 80% (hemolymph test) of adults. In serological analysis, 100% of G1 animals were seronegative and 80% were seropositive in G2. No riquetsial DNA was detected in the tissues of animals. In the second study, the profile of experimental infection caused by rickettsia in guinea pigs (animals from G1, G2 and G3) were analyzed, seeking to identify the clinical, histopathological and hematological profile. Blood samples for hematological analyzes were performed in the same periods of the previous study and the collection of tissue for histopathological analyzes, occurred at 10 and 28 DPI. In serology, animals from G1 and G3 were negative and 80% of the G2 positive. The observed hematological results were: G1 - leukopenia at 7 DPI, increased total plasma proteins (TPP) and decreased platelets at 7, 14 and 21 DPI, G2: leukocytosis, neutrophilia and monocytosis at 7 DPI, increased platelets at 14 DPI and decreased PPT at 21 DPI. Histopathology observed: G1 - diffuse splenic hemosiderosis at 28 DPI (20%), G2 - diffuse splenic hemosiderosis at 10 DPI (10%), diffuse pulmonary congestion at 10 and 28 DPI (30%) and multifocal splenic follicular hyperplasia at 28 DPI (20%); G3 - diffuse pulmonary congestion at 10 DPI (33%). It was concluded that nymphs of A. ovale presented vector competence in the transmission of R. parkeri (Atlantic Forrest strain) to guinea pigs, being possible to determine an acute infection of subclinical character in these hosts. / Este estudo teve como objetivos avaliar a capacidade de ninfas de Amblyomma ovale naturalmente infectadas com Rickettsia parkeri (cepa Mata Atlântica) em transmiti-la para Cavia porcellus (cobaios) e, analisar a infecção nestes animais. Utilizou-se 26 cobaios divididos em três grupos: G1 - 10 cobaios infestados com ninfas de A. ovale não infectadas, G2 - 10 cobaios infestados com ninfas de A. ovale naturalmente infectadas com R. parkeri (cepa Mata Atlântica) e G3 - 6 cobaios não infestados. Foi fixada uma câmara de infestação de carrapatos nos animais onde foram colocadas 25 ninfas de A. ovale infectadas ou não, de acordo com o grupo. No primeiro estudo foi avaliado a competência vetorial de ninfas de A. ovale na transmissão de R. parkeri (cepa Mata Atlântica) para C. porcellus (animais do G1 e G2). Após o período do parasitismo, as ninfas ingurgitadas foram coletadas e armazenadas em estufa B.O.D. Para a pesquisa de anticorpos anti-Rickettsia spp., coletou-se soros sanguíneos aos 7, 14, 21 e 28 dias pós infestação (DPI) sendo avaliados por meio de reação de imunofluorescência indireta (RIFI). Para a identificação da multiplicação desta riquétsia nos tecidos dos cobaios foi realizada a reação em cadeia da polimerase aos 7, 10, 14 e 28 DPI. Para verificar a competência vetorial das ninfas, analisaram-se os períodos parasitários, o percentual de ecdise, a sobrevivência transestadial e a RIFI. O período médio de parasitismo no G1 foi de 6,6 dias e, de 6 dias no G2. O percentual médio de ecdise (ninfa para adulto) foi de 95%. No G2, a sobrevivência desta riquétsia de forma transestadial foi confirmada em 100% (PCR) e 80% (teste de hemolinfa) dos adultos. Na análise sorológica, 100% dos animais do G1 foram soronegativos e 80% do G2 soropositivos. Não foi detectado o DNA riquetsial nos tecidos dos animais. No segundo estudo, avaliou-se o perfil da infecção experimental causada por esta riquétsia nos cobaios (animais do G1, G2 e G3), buscando identificar as alterações clínicas, perfil hematológico e histopatológico. As coletas sanguíneas para análises hematológicas foram realizadas nos mesmos períodos do estudo anterior e, as coletas de tecidos para análises histopatológicas, ocorreram aos 10 e 28 DPI. Na sorologia, os animais do G1 e G3 fora negativos e, 80% dos animais do G2 positivos. Os resultados hematológicos observados foram: G1 - leucopenia aos 7 DPI, aumento de proteínas plasmáticas totais (PPT) e diminuição de plaquetas aos 7, 14 e 21 DPI; G2: leucocitose, neutrofilia e monocitose aos 7 DPI, aumento de plaquetas aos 14 DPI e diminuição de PPT aos 21 DPI. Na análise histopatológica observou-se: G1 - hemossiderose difusa esplênica aos 28 DPI (20%); G2 - hemossiderose difusa esplênica aos 10 DPI (10%), congestão difusa pulmonar aos 10 e 28 DPI (30%) e hiperplasia folicular multifocal esplênica aos 28 DPI (20%); G3 - congestão difusa pulmonar aos 10 DPI (33%). Concluiu-se que ninfas de A. ovale apresentaram competência vetorial na transmissão de R. parkeri (cepa Mata Atlântica) para cobaios, sendo possível determinar uma infecção aguda de caráter subclínico nestes hospedeiros.

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