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Vulnérabilité à la schizophrénie : approche préclinique chez la souris porteuse d’une altération du gène codant la protéine STOP / From vulnerability to schizophrenia : preclinical approach in mice with an alteration of the gene coding for STOP proteinVolle, Julien 27 May 2010 (has links)
Au cours de ce travail, nous avons utilisé des souris ayant une délétion totale (KO) ou partielle (hétérozygotes) du gène codant la protéine STOP. La souris KO STOP constitue un modèle reconnu pour l’étude de la physiopathologie de la schizophrénie (SCH). Ce travail a permis d’étendre les données disponibles en mettant en évidence chez la souris KO STOP des troubles comportementaux qui miment les symptômes apparentés à l’ensemble des dimensions de la SCH. La comparaison des déficits chez des souris KO STOP générées à partir de différentes souches a permis de montrer que la délétion du gène STOP induit un phénotype robuste. De plus, notre travail permet de suggérer que, de par leur construction et leur phénotype, la souris hétérozygote STOP évoque la vulnérabilité à la SCH et pourrait constituer un modèle animal pertinent pour étudier les facteurs qui, interagissant avec une vulnérabilité génétique, favoriserait la décompensation psychotique. Dans cette optique, nous avons étudié l’influence de différents types de stress chroniques appliqués à différents moments clés du développement sur le phénotype de nos modèles animaux. Aucun des stress utilisés (isolement, stress chronique multiple, privation maternelle) n’a modifié le phénotype des souris hétérozygotes STOP à l’âge adulte, ni dans le sens de l’émergence ni dans celui d’une aggravation de troubles apparentés à la symptomatologie de la SCH. Ces résultats posent la question du type de stress, de son intensité et de la fenêtre temporelle où il doit être appliqué qui, associé à une altération génique donnée, validerait le modèle physiopathologique actuel basé sur une interaction délétère entre vulnérabilité et stress / In this work, we used mice with total (STOP null) or partial deletion (heterozygous) for the gene encoding the STOP protein. STOP null mice represent a recognized model for studying the physiopathology of schizophrenia (SCH). This work allowed us to extend the available phenotype by showing that STOP null mice exhibit various behavioural impairments mimicking symptoms corresponding to all SCH dimensions. By comparing the alterations present in STOP null mice generated from various strains, we showed that the STOP deletion induces a robust phenotype linked to SCH. In addition, through their construct and phenotype validity, the present work allows us to suggest that the STOP heterozygous mice evoke SCH vulnerability and could be a relevant model for studying the parameters which could favour SCH when interacting with a genetic vulnerability. In this context, the effects of chronic stress applied at different key steps of the mouse development were studied on the phenotype of our animal models. Any stress paradigm used (short-term isolation, chronic multiple stress, maternal deprivation) has been not able to alter the phenotype of STOP heterozygous mice measured in adulthood, neither by inducing nor by worsening alterations linked to SCH. According to the current SCH physiopathology hypothesis based on a deleterious interaction between susceptibility to SCH and stress, our findings raise the question regarding the type of stress paradigm, including stress intensity and the time window where it is applied, that could induce frank psychosis when interacting with a specific genetic alteration
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Prolactinemia em indivíduos com deficiência congênita e isolada do hormônio de crescimento / Prolactinemia in deficiecy and congenital isolated growth hormoneMenezes, Menilson 05 April 2013 (has links)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Growth hormone (GH) and prolactin share similarities in structure and function. We have previously shown that women with congenital isolated GH deficiency (IGHD) caused by a homozygous mutation in the GHRH receptor gene (GHRHR) (MUT/MUT) have a short reproductive life, with anticipated climacteric. At climacteric, they have lower prolactin levels than normal controls (N/N). Because they are able to breast feed, we hypothesized that this prolactin reduction is limited to climacteric, as result of lower estradiol exposure of the lactotrophs. The purposes of this work were to assess prolactin levels in broader age adults homozygous and heterozygous (MUT/N) for the mutation and in normal controls (N/N), and correlate them with determinants factors and insulin sensitivity. We enrolled 24 GH-naıve MUT/MUT (12 female), 25 MUT/N (14 female), and 25 N/N (11 female) subjects, aged 25 65 years. Anthropometric data and serum prolactin, estradiol, total testosterone, and sex hormone binding globulin (SHBG) were measured. Free testosterone was calculated. Prolactin levels were similar in the threeg groups. In males, testosterone and SHBG levels were higher in MUT/MUT in comparison to N/N. There was no difference in free testosterone among groups. In all 74 individuals, prolactin correlated inversely with age (p.0001) and directly with serum estradiol (p = 0.018). Prolactin levels in subjects with IGHD due to a homozygous GHRHR mutation are similar to heterozygous and normal homozygous, but total testosterone and SHBG are higher in male MUT/MUT, with no difference in free testosterone. The reduced prolactin level is limited to climacteric period, possibly due to reduced estrogen exposure. / O hormônio do crescimento (GH) e a prolactina possuem fortes semelhanças em estrutura e função. Nós demonstramos que as mulheres com nanismo grave devido a deficiência isolada de GH (DIGH), causada por uma mutação homozigótica (c.57 + 1G > A) no gene do receptor de GHRH (MUT/MUT), apresentam uma vida reprodutiva curta, e no momento do climatério têm níveis mais baixos de prolactina do que os contrôles normais (N / N). O fato delas serem capazes de amamentar no menacne, sugeriu a hipótese de que esta redução da prolactina seria limitada a climatério como resultado da redução da menor exposição do estradiol aos lactotrofos. Os objetivos deste trabalho foi avaliar os níveis de prolactina em uma faixa etária mais ampla em indivíduos adultos homozigóticos afetados e heterozigoticos (MUT/N), e correlacioná-los com fatores determinantes e a sensibilidade a insulina. Para tal, um estudo transversal analítico incluiu 74 individuos de ambos os sexos, 24 MUT/MUT (12 mulheres), 25 MUT/N (14 mulheres) e 25 N/N (11 mulheres), com idades entre 25 e 62 anos. Foram obtidos os dados antropoméricos (peso, altura e IMC), os exames hormonais (prolactina sérica, estradiol, testosterona total e SHBG) dosados, sendo a a testosterona livre calculada. Os níveis de prolactina foram semelhantes entre os três grupos. Os níveis de testosterona total e SHBG em individuos do sexo masculino foram maiores no MUT/MUT em comparação com N/N e MUT/N (p < 0,0001 e p < 0,05), respectivamente. Não houve diferença nos níveis de testosterona livre entre os grupos e gêneros. Em todos os 74 indivíduos, a prolactina sérica correlacionou-se inversamente com a idade (p < 0,0001) e diretamente com estradiol (p = 0,018). Conclusões: Os níveis de prolactina nos individuos com DIGH devido a uma mutação homzigotica no GHRHR são semelhantes aos indivíduos heterozigóticos e homozigóticos normais, mas a testosterona total e SHBG são mais elevados nos individuos do sexo masculino no grupo MUT/MUT, sem diferença nos níveis de testosterona livre. O nível reduzido de prolactina é limitado ao periodo do climatério, possivelmente devido a menor exposicção estrogênica.
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Using Phased Whole Genome Sequence Data to Better Understand the Role of Compound-Heterozygous Variants in Pediatric DiseasesMiller, Dustin B. 14 July 2021 (has links)
A compound-heterozygous variant occurs when a child inherits a variant from each parent, with these variants occurring at a different position within the same gene and on opposite homologous chromosomes. These inherited variants may result in two nonfunctional versions of the same gene. Compound-heterozygous variants cannot be identified unless a patients' DNA sequence data is phased. Phasing is a computationally demanding process that requires the use of multiple software tools in order to determine which nucleotide was inherited from which parent. First, in Chapter 1, we review the literature to better understand what research has been conducted on the role of compound-heterozygous variants in pediatric cancers and what methods are being used to identify them. In Chapter 2, we develop a pipeline to make it easier for us and other researchers to phase and identify compound-heterozygous variants using VCF files from trios or individuals. We then use this pipeline in Chapter 3 to survey the prevalence of compound-heterozygous variants across 7 pediatric disease types. We show the importance of identifying compound heterozygous and what information would be missed if this variant type was not included in study design. In Chapter 4, we develop a software tool to phase trio data using a combination of Mendelian inheritance logic and an existing phasing software program. We show that our software tool increases the total number of variants that can be phased. Finally, in Chapter 5, we use phased data of three nuclear families, each family having one child with pediatric cancer, to evaluate the potential to use inherited genomic variants to inform diagnostic decisions. The work contained within this dissertation shows the importance of not overlooking compound-heterozygous variants when trying to identify potentially causal genes in pediatric disease. In addition, this work provides software tools that are openly available for other researchers to use; these tools make it easier to phase patient DNA sequence data and to identify compound-heterozygous variants.
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Osteogenesis Imperfecta : Genetic and Therapeutic StudiesLindahl, Katarina January 2013 (has links)
Osteogenesis imperfecta (OI) is a heterogeneous disease of connective tissue, the cardinal symptom being fractures and severity ranging from mild to lethal. Dominant mutations in collagen I, encoded by COL1A1 and COL1A2, cause >90% of cases. To delineate genotype-phenotype correlations and pharmaco-genetic response, collagen I was sequenced in 150 unrelated Swedish families and clinical data were collected in Paper I. Mutation type, gene affected, and N- to C-terminal location correlated with phenotype and severity. Bisphosphonate response assessed by calculated yearly change in lumbar spine bone mineral density (BMD) was inversely related to age and BMD at treatment initiation. Mutations associated with a more severe phenotype exhibited an increased response after 2 years; however, all types of OI responded well. To investigate the effect of naturally occurring variations in collagen I, the only common coding single nucleotide polymorphism (rs42524 in COL1A2) was genotyped in 2004 healthy men in Paper II. Heterozygous genotype was associated with decreased BMD and an increased risk of stroke. An adolescent with repeated fractures despite a markedly high BMD harbored a unique C-terminal procollagen cleavage-site mutation in COL1A1, which motivated extensive investigations in concert with a similar COL1A2 case in Paper III. The probands were found to have impaired procollagen processing, incorporation of collagen with retained C-propeptide in matrix and increased mineral to matrix ratio, which demonstrates that C-propeptide cleavage is crucial to normal bone mineralization and structure. Bisphosphonate therapy has insufficient effect in OI, and as classical OI is a dominant disorder severe cases would benefit from silencing of the mutated allele. In Paper IV and V small interfering RNAs (siRNAs) were used to allele-specifically target primary human bone cells heterozygous for I) a coding polymorphism in COL1A2 and II) insertion/deletions in the 3’UTR of COL1A1 and COL1A2. Results were promising with altered allele ratios and decreased mRNA levels in the predicted fashion. To summarize, this thesis found that collagen I is crucial to bone and connective tissue and that collagen I mutations create markedly diverse phenotypes. Age, BMD and pharmaco-genetic effects influence the response to bisphosphonate therapy in individuals with OI; however, novel approaches are needed. Utilizing allele-specific siRNAs may be a way forward in the treatment of severe OI.
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Assessment of Cerebellar and Hippocampal Morphology and Biochemical Parameters in the Compound Heterozygous, Tottering/leaner MouseMurawski, Emily M. 2009 December 1900 (has links)
Due to two different mutations in the gene that encodes the a1A subunit of
voltage-activated CaV 2.1 calcium ion channels, the compound heterozygous
tottering/leaner (tg/tgla) mouse exhibits numerous neurological deficits. Human
disorders that arise from mutations in this voltage dependent calcium channel are
familial hemiplegic migraine, episodic ataxia-2, and spinocerebellar ataxia 6. The tg/tgla
mouse exhibits ataxia, movement disorders and memory impairment, suggesting that
both the cerebellum and hippocampus are affected. To gain greater understanding of the
many neurological abnormalities that are exhibited by the 90-120 day old tg/tgla mouse
the following aspects were investigated: 1) the morphology of the cerebellum and
hippocampus, 2) proliferation and death in cells of the hippocampal dentate gyrus and 3)
changes in basic biochemical parameters in granule cells of the cerebellum and
hippocampus.
This study revealed no volume abnormalities within the hippocampus of the
mutant mice, but a decrease in cell density with the pyramidal layer of CA3 and the hilus
of the dentate gyrus. Cell size in the CA3 region was unaffected, but cell size in the hilus of the dentate gyrus did not exhibit the gender difference seen in the wild type
mouse. The cerebellum showed a decrease in volume without any decrease in cerebellar
cellular density. Cell proliferation and differentiation in the subgranular zone of the
hippocampal dentate gyrus remained normal. This region also revealed a decrease in
cell death in the tg/tgla mice.
Basal intracellular calcium levels in granule cells show no difference within the
hippocampus, but an increase in the tg/tgla male cerebellum compared to the wild type
male cerebellum. There was no significant difference in granule cell mitochondrial
membrane potential within the wild type and mutant animals in either the hippocampus
or cerebellum. The rate of reactive oxygen species (ROS) production in granule cells
revealed no variation within the hippocampus or cerebellum. The amount of ROS was
decreased in cerebellar granule cells, but not granule cells of the hippocampus. Inducing
ROS showed no alteration in production or amount of ROS produced in the
hippocampus, but did show a ceiling in the amount of ROS produced, but not rate of
production, in the cerebellum.
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Die Bedeutung partieller 21-Hydroxylase- und 3beta-Hydroxysteroiddehydrogenasedefizienzen für die Ätiopathogenese von FertilitätsstörungenGhanaati, Zahra 12 March 2001 (has links)
Ziel der Untersuchungen war, zur Klärung der Ursachen einer während der letzten Jahrzehnte erhöhten Frequenz sowohl von PCOS als auch von IO beizutragen. Es war zu ermitteln, ob hormonelle Verschiebungen bei den Patienten nachweisbar und diese durch genetische und epigenetische Faktoren erklärbar sind. Ausgehend von dem Postulat, daß verminderte 21-OH- und 3beta-HSD-Aktivitäten als prädisponierende Faktoren von PCOS und IO angesehen werden, waren hormonanalytische Untersuchungen zur Ermittlung partieller 21-OH- bzw. 3beta-HSD-Defizienzen durchgeführt worden. Den eigenen Erfahrungen und Darstellungen der internationalen Literatur entsprechend befaßt sich ein Teil der Methodik mit der Entwicklung einer neuen, der üblichen 17alfa-OHP-Messung überlegenen Methode zur Ermittlung von 21-OH-Defizienzen durch 21-DOF-Bestimmung nach ACTH-Test im Blutplasma. Wir erhielten bei vier von 21 PCOS-Patientinnen und drei von acht Patienten mit IO erhöhte 21-DOF-, 21-DOF/F- bzw. 17alfa-OHP-Werte nach ACTH-Test, die auf partielle 21-OH-Defizienzen hinweisen. Zusätzlich wurden bei 12 PCOS-Patientinnen erhöhte basale DHEAS- oder DHEAS/F-Werte gefunden, die als Hinweise auf partielle 3beta-HSD-Defizienzen oder 17,20-Lyase-Hyperaktivität gedeutet wurden. In der Stichprobe der IO waren DHEAS oder DHEAS/F-Werte bei vier Patienten erhöht. Da bei vier der 12 Patientinnen mit PCOS und zwei von vier Patienten mit IO genetisch und endokrinologisch gleichzeitig eine partielle 21-OH-Defizienz nachgewiesen wurde, kann bei diesen Patienten eine partielle 3beta-HSD-Defizienz weitgehend ausgeschlossen werden. Es wurden molekulargenetische Untersuchungen für die 14 häufigsten Mutationen in CYP21 bei Cohorten mit AGS, PCOS und IO durchgeführt. Die Untersuchung der AGS-Patienten sollte dazu dienen, ein effizientes und schnelles System der Mutationssuche für diagnostische Zwecke zu etablieren. Es wurden die häufigsten, phänotypisch wirksamen Mutationen in CYP21 bei der Mehrzahl dieser Patientengruppe im homozygoten bzw. compound heterozygoten Zustand gefunden und eine deutliche Genotyp-Phänotyp-Korrelation festgestellt. Auch bei Patientinnen mit PCOS sowie bei IO, bei denen partielle 21-OH-Defizienzen nachweisbar waren, wurden Mutationen in CYP21 gefunden. Die hierbei heterozygot vorliegenden Mutationen waren dieselben, die homozygot oder compound heterozygot bei schweren Formen des AGS gefunden wurden. Es ergab sich eine Korrelation molekulargenetischer und hormonanalytischer Befunde bei AGS, PCOS sowie IO. Allerdings konnten bei der Mehrzahl der Fälle mit PCOS und mit IO weder Mutationen noch hormonelle Auffälligkeiten hinsichtlich partieller 21-OH-Defizienzen gefunden werden. Die jedoch bei vielen Patientinnen gefundenen erhöhten DHEAS- und DHEAS/F-Werte stimmen mit Untersuchungen überein, die parallel starke Zunahmen der Häufigkeit der Hemmung des Enzyms 3ß-HSD bzw. der Aktivierung der 17,20-Lyase bei PCOS-Patientinnen und der Prävalenz des PCOS selbst bei nach 1955 geborenen Frauen und von Spermatogenesestörungen bei nach 1960 geborenen Männern fanden. Die Ursache hierfür wird in der Beeinflussung der adrenalen und gonadalen Steroidhormonsynthese vor allem durch das Umweltteratogen DDT und seine Metaboliten gesehen. Weiterhin wurde der Umweltfaktor Streß diskutiert. Für die Ätiopathogenese der untersuchten Fertilitätsstörungen werden materno-fetale Mechanismen postuliert, worauf unsere sowohl molekulargenetischen als auch hormonanalytischen Befunde hinweisen. Insgesamt bestätigen die Ergebnisse unserer Arbeit die These, daß Leben auf der Interaktion von Genen und Umweltfaktoren beruht und daß Hormone dabei als Mediatoren wirken. In gen- oder umweltbedingten unphysiologischen Konzentrationen können sie während kritischer Entwicklungsphasen des neuroendokrinen Systems als Teratogene wirken und zu lebenslangen Reproduktionsstörungen führen. / This paper describes a mutational and hormonal screening in a cohort of 21 patients ultrasonically diagnosed with PCO. Our data show single heterozygous base pair CYP21 mutations in 4 patients. The four women with PCOS and CYP21 mutations also displayed clear signs of partial 21-hydroxylase deficiency through a significant rise in 21DOF or 17alfa-OHP plasma levels after ACTH stimulation. Azziz et al. have reported several heterozygous mutations in hyperandrogenic women with LO-CAH. Other studies report several heterozygous point mutations in hyperandrogenic woman who, however, were not examined for polycystic ovaries.The correlation between the hormone profiles and genetic screening results found with our patients underscores the latter s usefulness with PCOS patients. In contrast to the hormone profile, genetic screening is not influenced by external factors. The frequency of heterozygous CYP21 mutations is higher (19%) than in the normal population (5-8%), suggesting a link with PCOS in some cases. The ratio of LH/FSH was significantly raised in 43% of the cases. Most importantly, basal plasma DHEA-S levels and DHEA-S/F ratios were clearly increased, higher than the means +2SD in controls. This suggests a partial 3beta-hydroxysteroid dehydrogenase deficiency or 17,20 lyase hyperactivity. Other authors, however, were not able to find mutations in the corresponding genes. This could be explained by the fact that the DDT metabolite o,p DDD is a strong inhibitor of 3beta-HSD, and that DDT and its metabolites may be able to activate the 17,20 lyase, a cytochrome P450 enzyme. Furthermore, DDT has some oestrogen activity, and its perinatal administration can produce a PCOS-like syndrome in rats. Very significantly, there has not only been an approximately fourfold increased prevalence of PCO in women borne since 1955 in eastern Germany, following a massive prenatal exposure to DDT, but also a notable shift in the hormone profiles of those affected. A predominance of 3beta-HSD deficiencies and 17,20 lyase hyperactivity (70%) vs. 21-hydroxylase deficiency (23%) has emerged, in contrast with 21-hydroxylase deficiencies in 70% vs. 3beta-HSD deficiencies or 17,20 lyase hyperactivity in 14% for those born earlier than 1955. Similar results were obtained in this study for women with PCOS born since 1955, suggesting that the prenatal exposure of high amounts of DDT and its metabolites indeed appear to be responsible - at least in part - for the major increase in PCO and PCOS.
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Molecular and functional characterization of ABRAXAS and PALB2 genes in hereditary breast cancer predispositionBose, M. (Muthiah) 29 October 2019 (has links)
Abstract
Hereditary mutations in DNA damage response (DDR) genes often lead to genomic instability and ultimately tumor development. However, the molecular mechanism of how these DDR deficiencies promote genomic instability and malignancy is not well understood. Thus, the specific aim of this thesis is to identify the functional and molecular framework behind the elevated breast cancer risk observed in heterozygous PALB2 and ABRAXAS mutation carriers.
The heterozygous germline alteration in PALB2 (c.1592delT) causes a haploinsufficiency phenotype in the mutation carrier cells. Due to PALB2 haploinsufficiency, elevated Cdk activity and consequently aberrant DNA replication/damage response was observed in the PALB2 mutation carrier cells. Excessive origin firing that is indicative of replication stress was also seen in the PALB2 mutation carrier cells. In addition to replication stress, PALB2 mutation carrier cells also experience G2/M checkpoint maintenance defects. The increased malignancy risk in females associated with heterozygosity for the Finnish PALB2 founder mutation is likely to be due to aberrant DNA replication, elevated genomic instability and multiple different cell cycle checkpoint defects.
The heterozygous germline alteration in ABRAXAS (c.1082G>A) causes a dominant-negative phenotype in the mutation carrier cells. Decreased BRCA1 protein levels as well as reduced nuclear localization and foci formation of BRCA1 and CtIP was observed in the ABRAXAS mutation carrier cells. This causes disturbances in basal BRCA1-A complex localization, which is reflected by a restraint in error-prone DNA double-strand break (DSB) repair pathway usage, attenuated DNA damage response, deregulated G2/M checkpoint control and apoptosis. Most importantly, mutation carrier cells display a change in their transcriptional profile, which we attribute to the reduced nuclear levels of BRCA1. Thus, the Finnish ABRAXAS founder mutation acts in a dominant-negative manner on BRCA1 to promote genome destabilization in the heterozygous carrier cells. / Tiivistelmä
Perinnölliset muutokset DNA-vauriovasteen geeneissä johtavat usein genomin epävakauteen ja lopulta syövän kehittymiseen. Molekyylitason mekanismeja, joilla vauriovasteen vajaatoiminta ajaa genomin epävakautta ja syöpää, ei kuitenkaan ymmärretä kunnolla. Tämän väitöskirjan tavoitteena on tunnistaa solutoiminnan ja molekyylitason vaikuttajat heterotsygoottisten PALB2- ja ABRAXAS-geenimuutosten kantajien kohonneen rintasyöpäriskin taustalla.
Heterotsygoottinen ituradan suomalainen perustajamuutos PALB2-geenissä (c.1592delT) aiheuttaa haploinsuffisienssin kantajahenkilöiden soluissa. PALB2:n haploinsuffisienssin seurauksena kantajasoluissa havaittiin kohonnutta Cdk-proteiinin aktiivisuutta ja siitä johtuvaa kiihtynyttä DNA:n kahdentumista. PALB2-mutaatiota kantavissa soluissa nähtiin myös liiallista replikaation aloituskohtien käyttöä, mikä viittaa replikaatiostressiin. Replikaatiostressin lisäksi PALB2-mutaation kantajasoluilla havaittiin vaikeuksia ylläpitää solusyklin G2/M-tarkastuspisteen toimintaa. Näiden solutoiminnan poikkeavuuksien takia heterotsygoottisen PALB2 c.1592delT -mutaation kantajilla todettiin genomin epävakautta ja kohonnut syöpäriski.
Heterotsygoottinen ituradan mutaatio ABRAXAS-geenissä (c.1082G>A) aiheuttaa dominantti-negatiivisen fenotyypin mutaation kantajasoluissa. ABRAXAS-mutaatiota kantavissa soluissa havaittiin BRCA1-proteiinitasojen laskua sekä BRCA1- ja CtIP-proteiinien vähentynyttä lokalisaatiota tumaan ja DNA-vauriopaikoille. Tämä aiheuttaa häiriöitä BRCA1-A-kompleksin paikallistumisessa, mikä johtaa häiriöihin virhealttiiden DNA-kaksoisjuoste¬katkoksien korjausmekanismien käytössä, DNA-vauriovasteessa, G2/M-tarkastus-pisteen säätelyssä ja ohjelmoidussa solukuolemassa. Tärkeimpänä löydöksenä havaittiin mutaation kantajasoluissa muuttunut transkriptioprofiili, joka johtunee BRCA1-proteiinitasojen laskusta tumassa. Näin ollen suomalainen ABRAXAS-perustajamutaatio toimii dominantti-negatiivisena BRCA1:n suhteen, aiheuttaen genomin epävakautta heterotsygoottisissa kantajasoluissa.
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Jesus Christ’s humanity in the contexts of the pre-fall and post-fall natures of humanity: a comparative and critical evaluative study of the views of Jack Sequeira, Millard J. Erickson and Norman R. GulleyMwale, Emanuel 12 1900 (has links)
Bibliography: leaves 653-669 / Before God created human beings, He devised a plan to save them in case they sinned. In this plan, the second Person of the Godhead would become human. Thus, the incarnation of the second Person of the Godhead was solely for the purpose of saving fallen, sinful human beings. There would have been no incarnation if human beings had not sinned. Thus, the nature of the mission that necessitated the incarnation determined what kind of human nature Jesus was to assume.
It was sin that necessitated the incarnation – sin as a tendency and sin as an act of disobedience. In His incarnational life and later through His death on Calvary’s cross, Jesus needed to deal with this dual problem of sin. In order for Him to achieve this, He needed to identify Himself with the fallen humanity in such a way that He would qualify to be the substitute for the fallen humanity. In His role as fallen humanity’s substitute, He would die vicariously and at the same time have sin as a tendency rendered impotent. Jesus needed to assume a human nature that would qualify Him to be an understanding and sympathetic High Priest. He needed to assume a nature that would qualify Him to be an example in overcoming temptation and suffering.
Thus, in this study, after comparing and critically evaluating the Christological views of Jack Sequeira, Millard J. Erickson and Norman R. Gulley, I propose that Jesus assumed a unique post-fall (postlapsarian) human nature. He assumed the very nature that all human beings since humankind’s fall have, with its tendency or leaning towards sin. However, unlike other human beings, who are sinners by nature and need a saviour, Jesus was not a sinner. I contend that Jesus was unique because, first and foremost, He was conceived in Mary’s womb by the power of the Holy Spirit and was filled with the Holy Spirit throughout His earthly life. Second; He was the God-Man; and third, He lived a sinless life.
This study contributes to literature on Christology, and uniquely to Christological dialogue between Evangelical and Seventh-day Adventist theologians. / Philosophy, Practical and Systematic Theology / D. Phil. (Systematic Theology)
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