No description available.

No description available.

Small intestinal ileus affects up to half of all horses undergoing small intestinal surgery, leading to prolonged gastrointestinal (GI) transit time which can be life-threatening. Various prokinetic medications have been associated with varying side effects, questionable efficacy, and increased cost. Gum chewing as a form of sham feeding is used as a safe, effective, well-tolerated, and inexpensive way to ameliorate ileus following GI surgery in humans. Bit chewing for horses, an analogous activity, has been shown to significantly decrease GI total transit time (TTT); however, a direct effect of bit chewing on gastric emptying time (GET), small intestinal transit time (SITT), and total orocecal transit time (OCTT) has not been investigated. Our objective was to determine whether bit chewing increased small intestinal motility and decreased GE, SITT, and OCTT in clinically normal horses. Gastrointestinal motility was compared in horses that were bit chewing compared to control conditions (no bit chewing) in a prospective crossover design study using acetaminophen as a marker for GET and video endoscopy (ALICAM) capsules to determine GET, SITT, and OCTT. Bit chewing was well tolerated by all horses with no side effects noted. Bit chewing led to a shorter GET, SITT, and significantly shorter OCTT when compared to the control group (P = 0.015). Median times for bit chewing conditions were as follows: GE 2.86 hr, SITT 3.65 hr, and OCTT 6.15 hr whereas the median times for control conditions were as follows: GE: 5 hr, SITT 4.4 hr, and OCTT 9.92 hr. In summary, bit chewing proves to be a potential tool to hasten the motility of the oral GIT. It is safe, inexpensive, and potentially effective prokinetic treatment to horses suffering from postoperative ileus and further investigation is warranted. / Master of Science / Ileus, or a temporary lack of intestinal motility, is a common life-threatening problem in horses, especially following abdominal surgery. Current treatments have questionable efficacy and high cost. In human patients suffering from ileus, sham feeding with gum chewing that mimics normal food consumption with no food ingested has shown promising results in improving clinical signs and restarting gastrointestinal motility. Bit chewing, an analogous activity in horses, also decreases gastrointestinal (GI) total transit time (TTT). However, ileus in horses typically affects the small intestine, which is a part of the GI tract that has not been investigated in regards to bit chewing. Our objective was to determine whether bit chewing shortens gastric emptying time (GET), small intestinal transit time (SITT), and total orocecal transit time (OCTT) in clinically normal horses. Gastrointestinal motility was compared in horses that were bit chewing compared to control (no bit chewing) conditions in a prospective crossover design study using acetaminophen serum samples as a marker for GET and video endoscopy (ALICAM) capsules to determine GET, SITT, and OCTT. There were no adverse effects to bit chewing and OCTT was significantly shortened in horses when bit chewing compared to the control group. In summary, bit chewing is a tool to hasten gastrointestinal motility, specifically small intestinal motility, and it may be a safe, inexpensive, and effective treatment to improve small intestinal motility in horses suffering from ileus and further investigation is warranted.

Lidocaine continuous-rate infusions (CRI) are the most commonly used prokinetic in equine practice for the treatment of post-operative ileus and are also increasingly being used in pain management, such as in cases of severe laminitis, and are often used for prolonged durations. To date only limited time/concentration relationships of lidocaine administered as a short term (24hours) CRI to horses are reported. This study examined the time/concentration profile of lidocaine and its active metabolites (GX, MEGX) during a 96 hour lidocaine infusion in eight mature healthy horses. Serum lidocaine concentrations reached steady state by three hours and did not accumulate thereafter. The serum concentration of lidocaine was above the target therapeutic concentration (980ng/ml) only at 6 and 48 hours. The serum lidocaine concentration did not reach the range described as potentially causing toxicity (>1850ng/ml). The MEGX metabolite did not accumulate over time, while the GX metabolite accumulated significantly up to 48 hours and then remained constant. The serum concentrations of lidocaine, MEGX and GX were below the limit of detection within 24 hours of discontinuation of the infusion. None of the horses developed any signs of lidocaine toxicity during the study. It was concluded that the metabolism of lidocaine was not significantly impaired by prolonged infusion, contrasting with studies in dogs and humans. No adverse effects were observed in this study, which with the lack of lidocaine accumulation suggests that prolonged infusions are safe. However the accumulation of GX, a potentially toxic active metabolite, is cause for concern. / Master of Science

Bakgrund: Efter kirurgiska ingrepp förekommer postoperativt ileus (POI) som ett normalfysiologiskt tillstånd. POI definieras som ett avvikande mönster av gastrointestinal motilitet med karakteristiska symtom som illamående och kräkningar, uppspänd buk, samt utebliven gasavgång eller avföring. POI har påvisats påverka den postoperativa återhämtningen negativt. Syfte: Syftet med studien var att kartlägga interventioner som används för att minska durationen av postoperativt ileus vid svenska kirurgiska vårdavdelningar. Design: Enkätbaserad tvärsnittsstudie. Metod: Internetbaserad enkät skickades till vårdenhetschefer vid 86 kirurgiskt inriktade vårdavdelningar på fem universitetssjukhus i södra och mellersta Sverige. Resultat: Totalt 21 kirurgiska vårdavdelningar besvarade enkäten. Kunskaper kring innebörden och handläggningen av POI angavs som måttliga till goda. Den mest frekvent föreslagna interventionen inom omvårdnad var mobilisering och laxantia i olika former var den vanligaste farmakologiska interventionen. Konklusion: Omvårdnads- och farmakologiska interventioner av varierande evidensgrad används på svenska kirurgiska vårdavdelningar. Det tycks finnas ett behov av utbildning om och implementering av vetenskapliga kunskaper inom området för bedömning och handläggning av POI. / Background: Postoperative ileus (POI) occurs as a normal reaction to all forms of surgery. POI is defined as a deviant pattern of gastrointestinal motility with characteristic symptoms such as nausea and vomiting, abdominal distension and lack of gas or stool. Postoperative ileus has been shown to affect the postoperative recovery in a negative way. Aim: The aim of this study was to investigate the current use of interventions aimed to reduce the duration time of postoperative ileus in Swedish surgical wards. Design: A cross-sectional study. Method: A web-based survey was administered to matrons at 86 surgical wards in five university hospitals in the mid- and south of Sweden. Results: In total, 21 wards responded. The knowledge about the meaning and management of POI was perceived as moderate to good. The most frequent used nursing intervention was mobilization, and the most common pharmacological action proposed was laxatives of various sorts. Conclusion: Both nursing and pharmacological interventions with various grades of evidence are used in Swedish surgical wards. There are indications of a need for education and implementation of science-based knowledge within the area of assessing and managing POI.

Dysfunktion av den gastrointestinala motiliteten under det postoperativa skedet är en komplikation som kan orsakas till följd av kirurgiska ingrepp. Detta kan orsaka bukspänning, illamående samt smärta som komplicerar en ofullständig och snabb återhämtning efter kirurgiska ingrepp. Dysfunktion av den gastrointestinala motiliteten ökar risken för ytterligare postoperativa komplikationer som uttorkning, elektrolytrubbning och sepsis. Samt ökar riskerna för reoperation och återintagning på sjukhus efter utskrivning. Dysfunktion av den gastrointestinala motiliteten kan ge ökat lidande för patienter, skapa längre vårdtider, medföra högre sjukhuskostnader samt öka arbetsbelastningen för hälso- och sjukvårdspersonal. Syfte: Syftet var att beskriva sjuksköterskors omvårdnadsåtgärder vid dysfunktion av den gastrointestinala motiliteten hos patienter under det postoperativa skedet. Metod: Studien genomfördes som en allmän litteraturstudie med induktiv ansats. Resultat: Resultatet baserades på nio vetenskapliga artiklar med kvantitativa ansats. Utifrån kodning av meningsenheter från de vetenskapliga artiklarna framkom fyra huvudkategorier: Patientnära samtal, Mobilisering, Kost- och vätskeintag och Stimulering av den gastrointestinala motiliteten. Slutsats: Omvårdnadsåtgärder var en viktig del i förebyggandet för främjandet av den gastrointestinala motiliteten under det postoperativa skedet samt förbättrade möjligheten till ett tidigare tillfrisknande för patienter. Ett tidigt ingripande med omvårdnadsåtgärder till patienter som genomgått kirurgi bidrog det till minskat lidande, kortare vårdtider för patienterna samt minskad arbetsbelastning för hälso- och sjukvårdspersonal. / Dysfunction of the gastrointestinal motility in the post-operative stage is a complication that can be caused by surgical procedures. This can cause abdominal tension, nausea and pain that complicate an incomplete and rapid recovery after surgery. Dysfunction of gastrointestinal motility increases the risk of additional postoperative complications such as dehydration, electrolyte disruption and sepsis. As well as increasing the risk of reoperation and readmission in hospitals after discharge. Dysfunction of the gastrointestinal motility can lead to increased suffering for patients, create longer care times, bring higher hospital costs and increase the workload of healthcare professionals. Purpose: The purpose was to describe nursing care measures in dysfunction of the gastrointestinal motility to patients in the postoperative stage. Method: The study was conducted as a literature review with inductive approach. Result: The result was based on nine scientific articles with quantitative approaches. Based on the coding of opinion units from the scientific articles, four main categories emerged: Patient-related conversation, Mobilization, Diet and fluid intake and Stimulation of gastrointestinal motility. Conclusion: Nursing care measures were an important part of the prevention of the promotion of gastrointestinal motility in the postoperative stage, and improved the possibility of an earlier recovery for patients. Early intervention with nursing care measures for patients who have undergone surgery, it contributed to reduced suffering, shorter patient care times and reduced workload for healthcare professionals.

Sammanfattning Bakgrund: Postoperativ ileus är en allvarlig komplikation som kan uppstå, ofta till följd av smärtstillande läkemedel eller inflammation i tarmvävnaden. Tarmmotoriken avstannar och gas- och tarmavgång upphör. Symtomen är kraftiga buksmärtor, kräkningar och utspänd buk. Patienten utsätts för smärta, oro och förlängd sjukhusvistelse. Syfte: Att beskriva omvårdnadsåtgärder sjuksköterskan kan vidta för att förebygga postoperativ ileus samt att beskriva urvalsgrupperna i de inkluderade artiklarna. Metod: En litteraturstudie av deskriptiv design som grundas på elva vetenskapliga artiklar, samtliga med kvantitativ ansats. Huvudresultat: Omvårdnadsåtgärder som tidigt oralt vätske- och födointag, tugga tuggummi, mobilisering, gunga gungstol, akupressur och sjuksköterskeledd information har visats påskynda läkning av tarmen och reducera risken av postoperativ ileus. Omvårdnadsåtgärderna visade bäst resultat gällande tarmljud, tarmrörelser, gas- och tarmavgång och tolerans av normal föda. Slutsats: Omvårdnadsåtgärder har visats ha god effekt vid förebyggande av postoperativ ileus. Tydliga tecken på att tarmen återhämtat sig och att den postoperativa tarmparalysen upphört är gas- och tarmavgång, tarmljud och tarmrörelser, vilket samtliga påskyndas av omvårdnadsåtgärder. Att kontinuerligt ta del av kunskap genom forskning möjliggör för sjuksköterskan att utföra professionell omvårdnad av hög standard. Det är sjuksköterskans ansvar att utföra omvårdnadsåtgärder som förebygger risker, minskar lidande och främjar hälsa. / Abstract Background: Postoperative ileus is a serious complication, often due to pain relief medication or the inflammation of intestinal tissue. Bowel movements stop and the gastrointestinal tract ceases and the resulting symptoms are: severe abdominal pain, vomiting and tensioned abdomen. This result in pain, anxiety and long-term hospitalization for the patient. Aim: To describe nursing interventions which prevent postoperative ileus and describe the selection groups of included articles. Method: A literature study of descriptive design based on eleven articles, all of which take a quantitative approach. Main result: Nursing interventions such as; early oral fluid and food intake, chewing gum, mobilization, swing rocking chair, acupressure and nurse information have been shown to accelerate healing of the intestine and reduce the risk of postoperative ileus. Nursing interventions had best result relating to: bowel sound, bowel movement, defecation and tolerance of normal food. Conclusion: Nursing interventions have been shown effective in the prevention of postoperative ileus. Clear indications of intestinal recovery and postoperative paralysis recovery include first flatus, bowel sound, bowel movement and defecation, all of which are more likely to occur if the patient has had nursing intervention. Continuously acquiring knowledge through research enables nurses to perform professional care of high standard. It is the nurse's responsibility to carry out nursing interventions that prevent risks, reduce suffering and promote healing.

Propofol und Methohexital hemmen die Dünndarmperistaltik. Untersuchungen des Wirkmechanismus am Meerschweinchendünndarm in vitro Fragestellung: Die Hemmung der Darmmotilität durch Anästhetika und Pharmaka zur Analgosedierung von Patienten in der Intensivmedizin kann Ursache weiterer Komplikationen sein. Diese Arbeit untersucht, ob die Hypnotica Propofol und Methohexital einen Einfluss auf die intestinale Peristaltik haben. Methodik: Dünndarmsegmente des Meerschweinchens wurden in vitro in einer Vorrichtung perfundiert, die propulsive Peristaltik ermöglicht. Durch Registrierung des intraluminalen Drucks kann die Schwelle (peristaltic pressure threshold, PPT), ab der peristaltische Kontraktionen ausgelöst werden, bestimmt werden. Propofol, Methohexital, sowie mögliche Antagonisten, wurden den Dünndarmsegmenten extraserosal zupipettiert und die Änderungen der PPT registriert. Ergebnisse: Propofol und Methohexital beeinflussten die Dünndarmperistaltik auf unterschiedliche Art und Weise. Methohexital führte konzentrationsabhängig zu einem Anstieg der PPT, z.T. bis zur kompletten Hemmung der Peristaltik. Im Gegensatz dazu führte die Gabe von Propofol in keinem Fall zur kompletten Hemmung, es zeigte sich lediglich ein Anstieg der PPT. Die Hemmung durch Methohexital trat nach Naloxon und z.T. nach Bicucullin vermindert auf. Die Hemmung der Peristaltik durch Propofol war zumeist unbeeinflusst durch die verwendeten Antagonisten. Schlussfolgerung: Propofol und Methoxital hemmen konzentrationsabhängig die Dünndarmperistaltik des Meerschweinchens. Der hemmende Effekt von Methohexital scheint durch endogene Einflüsse und durch Bindung an vermittelt GABAA- Rezeptoren vermittelt zu werden. Die vorliegenden Daten weisen darauf hin, dass Hypnotika wie Propofol und Methohexital auch auf die menschliche Peristaltik einen hemmenden Einfluss haben und bei Intensivpatienten einen Ileus induzieren oder verschlimmern können. GABA- Rezeptoren scheinen hierbei eine untergeordnete Rolle zu spielen. / Propofol and Methohexital Inhibit Peristalsis in the Guinea-Pig Ileum In Vitro Introduction: Inhibition of gastrointestinal motility by drugs used for anaesthesia or sedation in critically ill patients in the ICU is a major problem leading to various complications. Thus this work examines whether the hypnotics Propofol and Methohexital exert an inhibitory effect on intestinal peristalsis. Methods: Peristalsis in isolated segments of the guinea-pig small intestine was elicited by distension of the gut wall through a rise of intraluminal pressure and recorded via the intraluminal pressure changes associated with the aborally moving peristaltic contractions. By Propofol, Methohexital and potential antagonists induced change of peristalsis was reflected by an increase/ decrease of the peristaltic pressure threshold (PPT). Results: Propofol and Methohexital impaired the peristalsis of the guinea-pig ileum in vitro in a different manner. Mehohexital concentration-dependently increased PPT, partly the peristaltic reflex was totally abolished. In contrast, propofol never caused complete inhibition of peristalsis. PPT was slightly elevated concentration-dependently with propofol. Inhibition by Methohexital was decreasesed by Naloxon and partly by Bicucullin. Inhibition by Propofol was mostly uninfluenced by the used antagonists. Conclusion: Propofol and Methohexital concentration-dependently impair intestinal peristalsis in the guinea-pig ileum. The inhibitory effect of methohexital seems to be mediated through activation of endogenous opioidergic pathways and through binding to GABAA- receptors. The data suggest that hypnotics such as propofol and methohexital also have an inhibitory effect on intestinal motility in humans and may induce or worsen intestinal atonia in ICU patients. At this GABA- receptors seem to play a tangential role.

No description available.

Post-operative ileus (POI) is the functional inhibition of propulsive intestinal motility which is a frequent occurrence following abdominal surgery in the horse and in humans. Rodent and human-derived data have shown that manipulation-induced activation of the resident muscularis externa (ME) macrophages in the intestine contributes to the pathophysiology of the disease. Most studies of the disease, specifically in the horse, have focussed on identification of risk factors, descriptive studies of the disease or the assessment of the efficacy of various therapeutic and prophylactic interventions. As a result, the proposed pathogenesis of equine POI is largely reliant on the translation of data from rodent models. The aims of this thesis were to identify macrophage populations in the normal equine gastrointestinal tract (GIT) and to study equine macrophage activation by stimulating equine bone marrow-derived macrophages (eqBMDMs) with lipopolysaccharide (LPS) as a model for intestinal macrophage activation. Firstly, the normal population of macrophages in the equine GIT was determined. Using CD163 as an immunohistochemical marker for macrophages. CD163+ve cells were present in all tissue layers of the equine intestine: mucosa, submucosa, ME and serosa. CD163+ve cells were regularly distributed within the ME, with accumulations adjacent to the myenteric plexus, and therefore to intestinal motility effector cells such as neurons and the Interstitial Cells of Cajal. The differentiation and survival of intestinal macrophages depends upon signals from the macrophage colony-stimulating factor (CSF-1) receptor. LPS translocation from the gut lumen is thought to be a key activator of ME macrophages. To provide a model for gut macrophages, a protocol was optimised to produce pure populations of equine bone marrow-derived macrophages (eqBMDMs) by cultivation of equine bone marrow in CSF-1. Macrophage functionality was assessed using microscopy, flow cytometry and phagocytosis assays. EqBMDMs responded to LPS stimulation with increases in expression of positive control genes, tumour necrosis factor alpha (TNF-α) and Indoleamine 2,3-dioxygenase (IDO1). The same mRNA was subjected to transcriptomic (RNA-Seq) analysis. Differential gene expression and network cluster analysis demonstrated an inflammatory response characterised by the production of pro-inflammatory cytokines such as interleukin 1 beta (IL-1β) and interleukin 6 (IL-6). However, in contrast to rodent macrophages, eqBMDMs failed to produce nitric oxide in response to LPS, showing species-specific variation in innate immune biology. Using these data, we compared gene expression in normal equine intestine and in intestine from horses undergoing abdominal surgery for colic (abdominal pain). Horses undergoing abdominal surgery showed evidence of increased expression of IL-1β, IL-6 and TNF-α in the mucosa and ME when compared to control tissue. Horses with post-operative reflux (POR), a clinical sign of POI, had increased gene expression of IL-1β, IL-6 and TNF-α compared to horses that did not develop POR following abdominal surgery. These preliminary data suggest that there is macrophage activation within the ME of the intestine during abdominal surgery in the horse, and that a greater activation state is present in horses that subsequently develop POR. The final part of this study was to investigate the effect of a long-acting form of CSF- 1, an Fc fusion protein (CSF1-Fc), as a potential treatment for POI using a mouse model. This work, performed in collaboration with another research group, found that mice lacking the C-C chemokine receptor type 2 (CCR2) gene, which is required for monocyte recruitment into tissues, had a longer recovery period following intestinal manipulation (IM) than wild type (WT) mice. With the administration of CSF1-Fc, infiltration of neutrophils to the ME was reduced and the number of macrophages in the ME was increased in both WT and CCR2-/- mice following IM. Administration of CSF1-Fc in CCR2-/- mice improved recovery of gastrointestinal transit three days following IM, to the same extent as WT mice. Network cluster analysis and RT-qPCR of the ME revealed clusters of genes induced and downregulated by CSF1-Fc, with increased expression of anti-inflammatory and pro-resolving genes after IM in WT and CCR2-/- mice following treatment with CSF1-Fc.