Made available in DSpace on 2016-04-04T18:28:13Z (GMT). No. of bitstreams: 1 Bruna Praxedes Yamamoto.pdf: 1114166 bytes, checksum: 921b9fccce6a71b605581f630188ea5c (MD5) Previous issue date: 2013-12-18 / Universidade Estadual Paulista J?lio de Mesquita Filho / The aim of this study is to clarify the dynamic, genesis and effects of inhibition as defense against anxiety in the individual s emotional development. It is a theoretical study with a conceptual and structural analysis of Winnicott s work, trying to show how he understood inhibition. We sought to clarify what was this defense to Winnicott, during his work, considering the psychoanalytic tradition described by Freud and Klein. This type of study contributes in theoretical and clinical terms, for example: currently, children who don t interact with each other, children who don t play at school or during a therapeutic session. In this study, we also could clarify different types of inhibition, for example: inhibition of play, inhibition of instincts, feeding inhibition, sex-inhibition and others. Also was possible point to some therapeutic care, individual or environmental, to treat these inhibitions. Although the operational aspects of clinical practice or clinical treatment weren t the objects of this research, the results showed a direction to treat these problems. In the conclusion, we emphasize that the beginnings of the individual s emotional development are the phases which are more susceptible for inhibition against anxiety to appear. Then, the environmental provisions (especially what the mother can provide to her baby) is of decisive importance. It is important that we note that the inhibition isn t always a pathology, because Winnicott believed that only a healthy child can organize defenses, such as inhibition, to deal the unconscious conflicts. / Esta pesquisa teve como objetivo explicitar a din?mica, a g?nese e os efeitos da inibi??o como uma defesa contra a ang?stia, advinda das rela??es interhumanas no desenvolvimento do indiv?duo, no ?mbito da teoria do desenvolvimento afetivo, do ponto de vista da obra de Winnicott. Para tanto, foi realizado um estudo te?rico, com uma an?lise conceitual e estrutural da obra de Winnicott, buscando mostrar como ele compreendeu a inibi??o. Tratou-se de explicitar o que o autor considera como sendo essa defesa, ao longo de sua obra, levando em considera??o a tradi??o psicanal?tica, especialmente as propostas por Freud e Klein. Este tipo de estudo torna poss?vel contribuir para abordar este problema, tanto em termos te?ricos, como cl?nicos, tal como, por exemplo, a presen?a desse fen?meno nas escolas, atualmente, em crian?as que n?o interagem com as outras, que n?o brincam na hora do recreio e t?m problemas de desenvolvimento cognitivo. Ainda que, os aspectos operativos da pr?tica cl?nica ou do tratamento cl?nico desses problemas n?o tenha sido objeto desta pesquisa, seus resultados apontam para uma orienta??o no trato dos problemas analisados. Neste estudo, tamb?m pudemos distinguir diversos tipos de inibi??o, tais como, a de brincar, a dos instintos, a da alimenta??o, da sexualidade, dentre outras. Al?m disso, alguns cuidados psicoterap?uticos foram distinguidos, sejam eles individual sejam do ambiente, necess?rios para que esse problema possa ser tratado. Nas considera??es finais, salientamos que as fases mais primitivas do desenvolvimento emocional s?o as mais suscet?veis ao uso da inibi??o como defesa contra a ang?stia, logo, a provis?o ambiental (especialmente aquilo que a m?e pode fornecer) tem uma import?ncia decisiva. ? importante ressaltar que nem sempre a inibi??o resulta numa patologia, uma vez que Winnicott acredita que somente uma crian?a saud?vel ? capaz de organizar defesas, inclusive a pr?pria inibi??o, para lidar pontualmente com os conflitos inconscientes.

Submitted by PPG Letras (letraspg@pucrs.br) on 2018-03-07T12:51:15Z No. of bitstreams: 1 TESE_Talita_Gonc?alves_Final.pdf: 1453683 bytes, checksum: b0ea4966191c6d14b03f960ac6de8cbe (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-03-08T14:03:07Z (GMT) No. of bitstreams: 1 TESE_Talita_Gonc?alves_Final.pdf: 1453683 bytes, checksum: b0ea4966191c6d14b03f960ac6de8cbe (MD5) / Made available in DSpace on 2018-03-08T14:12:26Z (GMT). No. of bitstreams: 1 TESE_Talita_Gonc?alves_Final.pdf: 1453683 bytes, checksum: b0ea4966191c6d14b03f960ac6de8cbe (MD5) Previous issue date: 2018-01-11 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / From a psycholinguistic perspective, this cross-sectional study falls on an interface between child bilingualism and cognition. The main objective of this study is to verify whether there are differences in bilingual (Brazilian Portuguese and Spanish speakers) and monolingual students (Brazilian Portuguese speakers) as the components of executive functions and meta-language. In order to test the hypothesis that bilingualism impacts in the development of ex-ecutive functions components and metalanguage, twenty-three (23) bilingual and 23 monolin-gual children, forty-six (n = 46), equally distributed according to age and school year - from first (1st) to third (3rd) year of elementary school), were examined through verbal and non-verbal tasks of executive functions and tasks of metalanguage. Verbal tasks that assess com-ponents of executive functions were Child Hayling Test, the subtests of Children?s Test of Pseudowords and Children Digit Span Test. Non-verbal tasks were Five Digit Test, Bells Test, Corsi Block Test and Trail Making Test for Children. Language Arbitrariness Test and Symbol Substitution Test were applied to evaluate the metalanguage. Forty-two (42) measures were derived from the nine proposed tasks, among which eighteen (18) have presented statis-tically significant differences, fourteen (14) have showed bilingual and four (4) monolingual advantage. Through the Mann Whitney statistical test, it was observed that there were no dif-ferences in the comparison between groups as the metalanguage measures. In general, consid-ering the measures that evaluate the components of the executive functions, the bilingual group has overcome the monolingual group in most of the found differences. It was found that there are differences between bilinguals and monolinguals regarding timing for adaptation to changes (cognitive flexibility). / A partir de uma perspectiva psicolingu?stica, este estudo transversal se insere em uma interface entre bilinguismo infantil e cogni??o. O objetivo principal deste estudo ? investigar se h? diferen?as em escolares bil?ngues (falantes de portugu?s brasileiro e espanhol) e monol?ngues (falantes de portugu?s brasileiro) quanto a componentes das fun??es executivas e ? metalinguagem. A fim de testar a hip?tese de que o bilinguismo impacta no desenvolvimento dos componentes das fun??es executivas e da metalinguagem, 23 crian?as bil?ngues e 23 monol?ngues (n=46), distribu?das igualmente conforme idade e ano escolar (do 1? ao 3? ano do Ensino Fundamental), foram examinadas por meio de tarefas verbais e n?o verbais de fun??es executivas e de tarefas de metalinguagem. As tarefas verbais que avaliam componentes das fun??es executivas foram Teste Hayling Infantil, subtestes Span de Pseudopalavras e de d?gitos. J? as tarefas n?o verbais foram Teste dos Cinco D?gitos, Teste de Cancelamento de Sinos, Teste Blocos de Corsi e o Teste de Trilhas Infantil. As tarefas de Arbitrariedade da L?ngua e Substitui??o de S?mbolos foram aplicadas para avaliar a metalinguagem. Dentre as nove tarefas propostas, derivaram-se 42 medidas, entre as quais 18 apresentaram diferen?as estatisticamente significativas (14 apontam vantagem bil?ngue e quatro vantagem monol?ngue). Por meio do teste estat?stico Mann Whitney, observou-se que n?o houve diferen?as na compara??o entre grupos quanto ?s medidas de metalinguagem. De modo geral, em rela??o ?s medidas que avaliam os componentes das fun??es executivas, o grupo bil?ngue superou o grupo monol?ngue na maioria das diferen?as encontradas. Constatou-se que h? diferen?as entre bil?ngues e monol?ngues quanto ao timing para adapta??o a mudan?as (flexibilidade cognitiva).

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-08-26T12:43:16Z No. of bitstreams: 1 474158 - Texto Completo.pdf: 30740052 bytes, checksum: 0b50c4fe801d696f16180d6fa641a96c (MD5) / Made available in DSpace on 2015-08-26T12:43:16Z (GMT). No. of bitstreams: 1 474158 - Texto Completo.pdf: 30740052 bytes, checksum: 0b50c4fe801d696f16180d6fa641a96c (MD5) Previous issue date: 2015-03-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / In this work, it was evaluated the possibility of using bark of black wattle (Acacia mearnsii) as a corrosion inhibitor in environments with presence of CO2 under high pressure. SAE 1010 carbon steel samples were exposed to two corrosive situations (with and without oxygen) under the conditions of 15 MPa and 70 ?C, with distinct tannin concentrations for different exposure times (168, 360 and 2160 hours). In order to characterize the specimens it was used infrared spectroscopy analysis, Xray diffraction, scanning electron microscopy by field emission, energy dispersive spectroscopy, mass loss and potentiodynamic polarization tests, determining the rate of corrosion and the efficiency tannin as of tannin as inhibitor. The results indicated that the tannin in deaerated environmental has no potential for use as an inhibitor once there was no significant reduction in corrosion rates and, also the formation of ferric-tannates film. Was not observed corrosion rates values, due mass loss, and were classified as severe corrosion, according to values of NACE RP-07-75 and was not inhibition efficiency values higher than 21 % obtained. Nevertheless, in aerated environmental, tannin worked as a barrier to diffusion of O2 and/or complexation of Fe2+ and Fe3+, providing favorable conditions to the formation of FeCO3 film with protective features. The corrosion rates were classified as moderate for 2160 hours, while 168 h and 360 h, as severe, also according to the values of NACE-RP-07-75, reaching values of up to 72% inhibition efficiency. / Neste trabalho, avaliou-se a possibilidade de utiliza??o do tanino vegetal da ac?cia negra (Acacia mearnsii) como inibidor de corros?o em ambientes com a presen?a de CO2 a alta press?o. Corpos de prova de a?o SAE 1010 foram expostos a dois meios corrosivos (sem e com O2) sob condi??es de 15 MPa e 70?C, com diferentes concentra??es de tanino para diferentes tempos de exposi??o (168, 360 e 2160 h). Para caracteriza??o foram realizadas an?lises de espectroscopia de infravermelho, difra??o de raios X, microscopia eletr?nica de varredura por emiss?o de campo, espectroscopia de energia dispersiva, perda de massa e ensaios de polariza??o potenciodin?mica, determinando-se a taxa de corros?o e a efici?ncia do tanino como inibidor. Os resultados indicaram que o tanino, em meio desaerado, n?o apresenta potencial para utiliza??o como inibidor, pois n?o houve redu??o significativa nas taxas de corros?o e tamb?m n?o se observou a forma??o do filme de ferrotanatos. Todos os valores de taxas de corros?o, por perda de massa, foram classificados como corros?o severa, segundo valores da NACE-RP-07-75 e n?o se obteve valores de efici?ncia de inibi??o maiores que 21 %. No entanto, em meio aerado, o tanino atuou como barreira para a difus?o de O2 e/ou na complexa??o dos ?xidos de Fe2+ e Fe3+, formando filmes de ferrotanatos, propiciando condi??es favor?veis para a forma??o do filme de FeCO3 com caracter?sticas protetoras. As taxas de corros?o foram classificadas como moderada para 2160 h, enquanto que para 168 h e 360 h, como severa, tamb?m segundo valores da NACE-RP-07-75, atingindo-se valores de at? 72 % de efici?ncia de inibi??o.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-05-05T12:14:40Z No. of bitstreams: 1 2010 - Ricardo Ant?nio Tavares de Macedo.pdf: 1168736 bytes, checksum: f5cc6d2a34b5331058f2910d1fe16678 (MD5) / Made available in DSpace on 2017-05-05T12:14:40Z (GMT). No. of bitstreams: 1 2010 - Ricardo Ant?nio Tavares de Macedo.pdf: 1168736 bytes, checksum: f5cc6d2a34b5331058f2910d1fe16678 (MD5) Previous issue date: 2010-10-29 / Bean plants can get nitrogen from soil organic matter, fertilizer or biological fixation of atmospheric N2 (BNF). However, the biological process in this culture has shown low efficiency under field conditions. As a result, many farmers do not inoculate the seeds with efficient strains of rhizobia, being fertilizer the main source of N to this culture. Besides the absence of inoculation, high doses of N applied to the soil inhibit nodulation and BNF. However, the reasons for this inhibition are not fully understood, especially because the N is uptaked as nitrate (NO3 -) and ammonium (NH4 +). Whereas the rhizosphere of plants grown with nitrate is alkalized and ammonium is acidified, the aim of this study was evaluate the effect of pH changes in rhizosphere (pHR), caused by the metabolism of these N sources, in start of nodules formation. The experiments were conducted in growth chamber with average brightness of 400 lux, photoperiod of 12/12 hours (light/dark) and temperature 25?C ? 2?C. Plants were grown in pots with sand, soils with low (8%) and medium (25%) clay content and nutrient solution. In the 1st test (salinity) was found that ammonium had the greatest increase in electrical conductivity of the sand (EC), obtained with a 60 KgN ha-1 the value of 1.655 mS cm-1 with ammonium and 1.301 mS cm-1 with nitrate. In the following assay were compared two contrasting cultivars in capacity of nodulate, which confirmed the high (Ouro Negro) and low (Rio Tibagi) capability. As among cultivars were not significant differences in the values of pHR, in others experiments was evaluated Ouro Negro only. In this assay and in others ammonium was the source of N with more capacity of rhizosphere acidify and inhibit nodulation. Through regression analysis for each N source, was given the dose of total inhibition of nodulation (critical level), pHR at this dose and the dose which was 80% of maximum nodulation (dose of coexistence). In general, was verified the lowest doses of critical level and coexistence using ammonium than with nitrate. In the sand test (Ouro Negro sampled 20 DAE) for ammonium and nitrate the doses of coexistence was 13 and 58 KgN ha-1 and the values of pHR was 3,95 and 5,59, respectively. In test with different soil types (20 DAE), the dose of coexistence was lower with ammonium than nitrate, being in soil with lower clay content 18 e 41 KgN ha-1 and soil with higher clay content 15 e 27 KgN ha-1, respectively. The values of pHR for ammonium and nitrate were 4,69 and 6,08 in soil with lower clay content and 4,23 and 4,63 in soil with higher clay content, respectively. In this test the neutralizers alleviated acidification of the rhizosphere and nodulation was optimized. In test with leaf application of N, ammonium also more acidify the rhizosphere and promoted less nodulation (pHR 4,15 and 14 nodules plant-1) compared to nitrate (pHR 5,03 and 27 nodules plant-1). In the 6th experiment (20 DAE) the dose of 115 kgVO4 ha-1 allowed that highest dose of ammonium did not make changes in pHR, allowing also the dose of coexistence of 46 KgN ha-1 for ammonium as source of N. In all tests, the highest N rates from all sources completely inhibited nodulation, suggesting that these doses were the occurrence of others inhibiting factors independent of pH and not measured. / O feijoeiro pode adquirir nitrog?nio da mat?ria org?nica do solo, de adubos ou da fixa??o biol?gica do N2 atmosf?rico (FBN). Entretanto, nesta cultura o processo biol?gico tem apresentado baixa efici?ncia nas condi??es de campo. Em fun??o disso, muitos produtores n?o inoculam as sementes com estirpes eficientes de riz?bio, sendo a aduba??o a principal fonte de N para esta cultura. Al?m da aus?ncia da inocula??o, altas doses de N aplicadas no solo inibem a nodula??o e a FBN. No entanto, as causas para esta inibi??o n?o s?o totalmente esclarecidas, especialmente pelo fato do N ser absorvido na forma de nitrato (NO3 -) e am?nio (NH4 +). Considerando que a rizosfera da planta crescida com nitrato ? alcalinizada e com am?nio ? acidificada, este trabalho teve o objetivo de avaliar o efeito das altera??es de pH rizosf?rico (pHR), causadas pelo metabolismo destas fontes de N, no in?cio da forma??o de n?dulos. Os experimentos foram realizados em c?mara de crescimento com luminosidade m?dia de 400 Lux, fotoper?odo de 12/12 horas (luz/escuro) e temperatura de 25?C ? 2?C. As plantas foram crescidas em vasos com areia, solos com baixo (8%) e m?dio (25%) teor de argila e em solu??o nutritiva. No 1? ensaio (salinidade) foi verificado que o am?nio causou maior eleva??o da condutividade el?trica da areia (CE), obtendo-se na dose de 60 kgN ha-1 o valor de 1.655 ?S cm-1 com am?nio e de 1.301 ?S cm-1 com nitrato. No ensaio seguinte foram comparadas 2 cultivares contrastantes quanto ? capacidade nodular, que confirmaram a alta (Ouro Negro) e a baixa (Rio Tibagi) capacidade. Como entre as cultivares n?o ocorreram diferen?as expressivas nos valores de pHR, nos demais experimentos avaliou-se apenas a Ouro Negro. Neste ensaio e nos demais o am?nio foi a fonte de N que mais acidificou a rizosfera e inibiu a nodula??o. Atrav?s de an?lises de regress?o para cada fonte de N foi determinada a dose de inibi??o total da nodula??o (n?vel cr?tico), pHR nesta dose e a dose onde ocorreu 80% da m?xima nodula??o (dose de conviv?ncia). Em geral, foram observadas as menores doses de NC e de conviv?ncia com uso do am?nio do que com nitrato. No ensaio em areia (cv Ouro Negro coletada aos 20 DAE), para am?nio e nitrato as doses de conviv?ncia foram 13 e 58 kgN ha-1 e os valores de pHR 3,95 e 5,59, respectivamente. No ensaio em diferentes tipos de solo (20 DAE), a dose de conviv?ncia foi menor com am?nio do que com nitrato, sendo no solo com menor teor de argila 18 e 41 kgN ha-1 e no solo com maior teor de argila 15 e 27 kgN ha-1, respectivamente. J? os valores de pHR para am?nio e nitrato foram 4,69 e 6,08 no solo com menor teor de argila e 4,23 e 4,63 no solo com maior teor de argila, respectivamente. Neste ensaio os neutralizantes aliviaram a acidifica??o da rizosfera e otimizaram a nodula??o. No ensaio com aplica??o de N via foliar, o am?nio tamb?m acidificou a rizosfera e promoveu menor nodula??o (pHR 4,15 e 14 n?dulos planta-1) em rela??o ao nitrato (pHR 5,03 e 27 n?dulos planta-1). No 6? experimento (20 DAE) a dose de 115 kgVO4 ha-1 permitiu que na mais alta dose de am?nio o pHR n?o sofresse altera??es, permitindo tamb?m a dose de conviv?ncia de 46 kgN ha-1 para o am?nio. Em todos os ensaios, as mais altas doses de N de todas as fontes suprimiram a nodula??o, sugerindo que nestas doses ocorreram fatores inibidores independentes do pH e n?o mensurados.

Made available in DSpace on 2014-12-17T15:01:24Z (GMT). No. of bitstreams: 1 JulianaCSA_DISSERT.pdf: 2885300 bytes, checksum: 18768d46af6895ea630731a4c28b4d2d (MD5) Previous issue date: 2010-12-17 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The food industry is interested in natural products. Anthocyanins are phenolic antioxidants of great importance with health-relevant applications. Several studies have linked the intake of fruits and vegetables with reduced risk of chronic degenerative diseases because of its antioxidant properties. This study aimed to compare different strategies for obtaining natural pigments from red jambo (Syzygium malaccence) and analyze its functional potential. Two different strategies were studied: (1) solid-liquid extraction (SLE) in reactor with controlled parameters, (2) powder obtention. The investigation of the functional potential was conducted taking into account the total phenolic content (TPC), the antioxidant activity (AA), the total anthocyanins concentration (TA) and α-amylase and α-glucosidase inhibition. The best extracts obtained by SLE showed TPC of 174.15 mg GAE/100g, AA of 3.56 μmol Trolox eq/g and TA of 133.59 mg cyd-3-glu/100 g. The best results for the second strategy were TPC of 1024.22 mg GAE/100 g, AA of 29.03 μmol Trolox eq/g and TA of 1193.41 mg cyd-3- glu/100 g. It was observed moderate amylase inhibition (26.30%) and high glucosidase inhibitory activity (97.47%). Skin extracts showed, in general, superior results when compared to whole red jambo, with superior values for dehydrated products. Based on our result, red jambo can be considered as a rich source of phenolic antioxidants, as well on amylase and glucosidase inhibitors / A ind?stria aliment?cia demonstra forte interesse em estudos de extra??o envolvendo produtos naturais. A antocianina ? um fen?lico antioxidante de grande import?ncia e atua??o no organismo dos seres vivos. V?rios estudos relacionam ? ingest?o de frutas e vegetais com a diminui??o do risco e desenvolvimento de doen?as cr?nicodegenerativas em fun??o de suas propriedades antioxidantes. Este trabalho teve como objetivo comparar diferentes estrat?gias de obten??o do corante da casca e do jambo inteiro sem caro?o e analisar seu potencial funcional. Duas diferentes estrat?gias foram estudadas: (1) extra??o s?lido-l?quido em reator enjaquetado com controle de par?metros; (2) obten??o do p?. A investiga??o do potencial funcional foi realizada por meio de an?lises quanto ao teor de compostos fen?licos totais (CFT), a atividade antioxidante (AA), a concentra??o de antocianinas totais (AT) e a inibi??o das enzimas α-amilase e α-glicosidase. Os extratos com os melhores resultados para a estrat?gia 1 foram para CFT de 174,15 mg GAE/100 g, para a AA de 3,56 μmol Trolox eq/g e para AT de 133,59 mg ci-3-gli/100 g. Os melhores valores para a estrat?gia 2 foram para CFT de 1024,22 mg GAE/100 g, para AA de 29,03 μmol Trolox eq/g e para AT de 1193,41 mg ci-3-gli/100 g. A a??o inibit?ria das enzimas α-amilase (26,30%) e α-glicosidase (97,47%) mostraram-se potentes. Os extratos da casca apresentaram, de maneira geral, resultados superiores quando comparados aos valores dos extratos do jambo inteiro e as maiores quantifica??es foram obtidas dos produtos desidratados. As amostras analisadas exibiram fontes satisfat?rias de fen?licos antoci?nicos, com potente capacidade antioxidante e atividade inibit?ria das enzimas α-amilase e α-glicosidase

Made available in DSpace on 2014-12-17T15:41:43Z (GMT). No. of bitstreams: 1 ThiagoRR.pdf: 1895833 bytes, checksum: 59d0cd6d03df2b5c8165e3ae0bf39a80 (MD5) Previous issue date: 2008-04-25 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / An interesting development in surfactants science and technology is their application as corrosion inhibitors, since they act as protective films over anodic and cathodic surfaces. This work aims to investigate the efficiency of saponified coconut oil (SCO) as corrosion inhibitor and of microemulsified system (SCO + butanol + kerosene oil + distilled water), in saline medium, using an adapted instrumented cell, via techniques involving linear polarization resistance (LPR) and mass loss coupons (MLC). For this, curves of efficiency versus SCO concentration (ranging between 0 and 75 ppm) have been constructed. According to the obtained results, the following efficiency levels were reached with OCS: 98% at a 75 ppm concentration via the LPR method and 95% at 75 ppm via the MLC method. The microemulsified system, for a concentration of 15 ppm of SCO, obtained maximum inhibition of 97% (LPR) and 93% (MLC). These data indicate that it is possible to optimize the use of SCO in similar applications. Previous works have demonstrated that maximal efficiencies below 90% are attained, typically 65% as free molecules and 77% in microemulsified medium, via the LPR method in a different type of cell. Therefore, it can be concluded that the adapted instrumented cell (in those used methods) showed to be an important tool in this kind of study and the SCO was shown effective in the inhibition of the metal / Tensoativos v?m sendo alvo de interesse como inibidores de corros?o, pois funcionam como pel?culas protetoras sobre ?reas an?dicas e cat?dicas. Este trabalho teve como objetivo avaliar a efici?ncia de inibi??o ? corros?o do tensoativo ?leo de coco saponificado (OCS) e do sistema icroemulsionado (OCS + butanol + querosene + ?gua destilada), em meio salino, utilizando-se uma c?lula instrumentada adaptada. A efic?cia do OCS foi avaliada utilizando-se os m?todos de resist?ncia de polariza??o linear (LPR) e cupons de perda de massa (CPM), tendo sido constru?das curvas de efici?ncia versus concentra??o de OCS (variando de 0 a 75 ppm). De acordo com os resultados obtidos, verificou-se que este tensoativo apresenta a seguinte ordem de efici?ncia de inibi??o ? corros?o: para uma concentra??o de 75 ppm, 98% pelo m?todo LPR e 95% pelo m?todo CPM. O sistema microemulsionado, para uma concentra??o de 15 ppm de OCS, obteve inibi??o m?xima de 97% (LPR) e 93% (CPM). Atrav?s deste trabalho foi poss?vel otimizar o uso do tensoativo OCS, que em trabalhos anteriores mostrou efic?cia m?xima inferior a 90% (63% na sua forma livre e 77% na sua forma microemulsionada, pelo m?todo LPR, em um outro tipo de c?lula). Conclusivamente, pode-se dizer que a c?lula instrumentada adaptada (nos m?todos utilizados) provou ser uma ferramenta importante neste tipo de estudo e o OCS mostrou-se efetivo na inibi??o do metal

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-05-17T11:44:30Z No. of bitstreams: 1 2016 - Francis Barbosa Ferreira.pdf: 4527522 bytes, checksum: 6a5a6589610ff851e68801c3ec05e3c9 (MD5) / Made available in DSpace on 2017-05-17T11:44:30Z (GMT). No. of bitstreams: 1 2016 - Francis Barbosa Ferreira.pdf: 4527522 bytes, checksum: 6a5a6589610ff851e68801c3ec05e3c9 (MD5) Previous issue date: 2016-08-04 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / In this work, semi and thiosemicarbazones selected from the LaDMol-QM library, were used to study their interactions with a metalloproteinase from the snake Bothrops pauloensis (BpMP-I) by molecular modelling and enzymatic inhibition assays with the toxin. The crystalographic structure of BaPI (PDB code: 2W12) was used as a mold to build the 3D model of BpMP-I by homology modeling. The theorical model of BpMP-I showed good quality parameters and was used in a subsequent molecular modeling study. The thiossemicarbazones showed better molecular docking results and in vitro enzymatic inhibitions assays than semicarbazones. Studies by semi-empirical methods indicate a positive enthalpy of interaction, suggesting that the enzyme inhibition by these compounds must be a entropy-driven process. The results were used together to select the LDQM-IN-23 compound and propose rationally designed modifications to improve the interactions with the toxin. The study of the catalytic site of BpMP-I showed that there is an adjacent pocket with amino groups of the peptide bonds available for interaction. All results were used together to design structural changes, aiming the enhancing of the interaction with toxin. Therefore, was proposed the insertion of the carboxyl group with different spacers, containing 2 (LDQM-IN- 23b) and 3 methylene groups (LDQM-IN-23c). The docking results and semi-empiric optimization showed that there was a considerable improvement in the interaction for the modified compounds. The modified compounds were synthesized and tested for biological and enzymatic inhibition activity. It was observed that the IC50 values have improved: the original molecule, LDQM-IN-23 has an IC50 of 3,011 ?M and the modified molecules have IC50 of 79.12 (LDQM-IN-23b) and 1.77 ?M (LDQM-IN-23c). These molecules were tested for inhibition of hemorrhagic activity induced by Bothropoidin, a P-III class metalloproteinase, and by the B. pauloensis whole snake venom. The three molecules can inhibit the hemorrhagic activity induced by isolated toxin and whole venom, and LDQM-IN- 23c showed higher efficiency compared with the other two, and in a rate of 1:10 (w/w venom/inhibitor) the inhibition of the hemorrhagic activity was 100%. A molecular docking study of this lead compound with Snake Venom Metalloproteases (SVMPs) from different snake species and genera showed that this molecule can effectivelly interact with these SVMPs. / Neste trabalho, foram utilizadas semi e tiossemicarbazonas, selecionadas na quimioteca do LaDMol-QM (Dequim-UFRRJ), para o estudo das intera??es destas com o s?tio ativo de uma metaloprotease da pe?onha da serpente Bothrops pauloensis por modelagem molecular e ensaios de inibi??o da atividade enzim?tica e biol?gica sobre a toxina. A estrutura cristalogr?fica de uma metaloprotease (BaPI) complexada com um inibidor (um peptideomim?tico) (c?digo PDB 2W12) foi utilizada como molde para a constru??o do modelo 3D da metaloprotease da pe?onha de B. pauloensis (BpMP-I). O modelo 3D te?rico da BpMP-I, in?dito para esta toxina, apresentou bons par?metros de qualidade, sendo considerado adequado para estudos de planejamento de ligantes baseado na estrutura. As tiossemicarbazonas obtiveram melhores resultados, quando comparados com os resultados das semicarbazonas, tanto para os ensaios de docagem molecular quanto para estudos de inibi??o da atividade enzim?tica in vitro. Estudos por m?todos semiemp?ricos indicam uma entalpia de intera??o positiva, sugerindo que a inibi??o enzim?tica por estes compostos deve ser um processo controlado entropicamente. Os resultados foram utilizados para selecionar o derivado LDQM-IN-23 e propor modifica??es estruturais planejadas racionalmente, visando melhorar a intera??o deste com a toxina. O estudo do s?tio catal?tico da metaloprotease mostrou que esta possui uma cavidade adjacente com grupos amino das liga??es pept?dicas dispon?veis para intera??o. Foi proposta, ent?o, a inser??o de um grupo carboxilato com diferentes espa?adores, 2 (LDQM-IN-23b) e 3 grupos metileno (LDQM-IN-23c). Os resultados de docagem e otimiza??o semi-emp?rica mostraram que houve uma melhora consider?vel na intera??o dos ligantes modificados, os quais foram sintetizados e testados para as atividades de inibi??o enzim?tica e biol?gica. Na inibi??o enzim?tica, houve melhora da CI50 com o aumento do espa?ador. O composto LDQM-IN-23 tem CI50 de 3011,00 ?M e os compostos modificados possuem a CI50 de 79,12 (LDQM-IN-23b) e 1,77 ?M (LDQM-IN- 23c). Estes compostos foram testados para a inibi??o da atividade hemorr?gica in vivo induzida pela Botropoidina, uma metaloprotease da classe P-III, e pela pe?onha bruta de B. pauloensis. Os tr?s compostos conseguiram inibir a atividade hemorr?gica induzida pela toxina isolada e pela pe?onha, sendo que o composto LDQM-IN-23c mostrou maior efici?ncia, quando comparado com os outros dois, e para a propor??o de 1:10 (m/m pe?onha/inibidor) a inibi??o da atividade foi de 100%. Foi realizado um estudo de docagem deste composto l?der com outras metaloproteases de pe?onha de serpentes (SVMPs ? Snake Venom Metalloproteinases), de esp?cies e g?neros diferentes, mostrando que este ligante consegue interagir com outras SVMPs e ? um candidato para inibir a atividade hemorr?gica de SVMPs presentes na pe?onha, n?o s? de B. pauloensis, mas de outras serpentes

Made available in DSpace on 2014-12-17T14:03:41Z (GMT). No. of bitstreams: 1 TaffarelMT_DISSERT.pdf: 2213102 bytes, checksum: 779f70cd3a9747e4f3a386630bd3d1cc (MD5) Previous issue date: 2013-04-09 / Funda??o de Apoio ? Pesquisa do Estado do Rio Grande do Norte / Oilseeds are a high-value natural resource, due to its use as a substitute for petroleum. However, the storage time can reduce seed viability and oil quality. Therefore, scientific efforts have been made to provide a increment of storage time, germination rates and plant establishment of high-value oilseeds. The seedling establishment depends of the plant pass over the functional transition stage, characterized by a metabolic change from heterotrophic condition to autotrophic one. The storage oil mobilization is performed by β-oxidation process and the glyoxylate cycle. Also, the functional transition involves acclimation to photosynthetic condition, which generally includes the participation of antioxidant system and the reactive oxygen species, the latter are produced in various reactions of primary and secondary metabolism. In the present study, Catalase was inhibited during the functional transition of sunflower and safflower, after were performed many analyzes to elucidate the effects caused on the SOD and APX antioxidant systems. Also, were checked the changes in expression pattern of the glyoxylate cycle enzymes markers, ICL and MLS. It was observed that after CAT inhibition, the SOD and APX antioxidant systems allow the seedling establishment. Besides, was verified that both oilseeds can be accelerate the reverse mobilization and the photosynthetic establishment when Catalase activity has dramatically decreased / As sementes oleaginosas armazenam ?leos, um recurso natural de alto valor econ?mico devido a sua aplicabilidade como substituto aos derivados do petr?leo. Por?m, o tempo de armazenamento pode inviabilizar a semente provocando a redu??o da qualidade do ?leo e perda da viabilidade da semente. Por este motivo, esfor?os cient?ficos t?m sido realizados para proporcionar um maior tempo de armazenamento e incrementar taxas de germina??o e estabelecimento destas plantas com alto valor econ?mico. O estabelecimento da pl?ntula depende da capacidade do vegetal transpassar a etapa de transi??o funcional, caracterizada pela mudan?a do estado heterotr?fico para o autotr?fico. O consumo das reservas de ?leo depende das vias de β-oxida??o e do ciclo do glioxilato. No entanto, o processo de transi??o envolve a aclimata??o do vegetal ? condi??o de organismo fotossintetizante, que em geral conta com a participa??o fundamental do sistema antioxidante do vegetal devido ? produ??o de esp?cies reativas de oxig?nio em v?rias rea??es do metabolismo prim?rio e secund?rio. Neste estudo, a Catalase foi inibida durante a transi??o funcional de girassol e c?rtamo para analisar os efeitos provocados nos sistemas antioxidantes de APX e da SOD e verificar as modifica??es no padr?o de express?o das enzimas marcadoras do ciclo do glioxilato, ICL e MLS. Constatou-se que diante da inibi??o da Catalase o sistema antioxidante da APX e da SOD permitem o estabelecimento da pl?ntula. Verificou-se tamb?m que as duas oleaginoasas parecem acelerar a mobiliza??o de reservas e o estabelecimento fotossint?tico quando a Catalase tem a atividade drasticamente reduzida