The use of spectrofluorometers to examine nanomaterials is quite popular using either fluorescence or synchronous measurements. However, understanding how a material’s optical properties can influence spectral acquisition are of great importance to accurately characterize nanomaterials. This dissertation presents a series of computational and experimental studies aimed at enhancing the quantitative understanding of nanoparticle interactions with matter and photons. This allows for more reliable spectrofluorometer based acquisition of nanoparticle containing solutions. Chapter I presents a background overview of the works described in this dissertation. Correction of the gold nanoparticle (AuNP) inner filter effect (IFE) on fluorophore fluorescence using PEGylated AuNPs as an external reference method is demonstrated in Chapter II. The AuNP IFE is corrected to quantify tryptophan fluorescence for surface adsorbed proteins. We demonstrate that protein adsorption onto AuNPs will only induce ~ 20% tryptophan fluorescence reduction instead of the commonly assumed 100% reduction. Using water Raman intensities to determine the effective path lengths of a spectrofluorometer for correction of fluorophore fluorescence is discussed in Chapter III. Using Ni(NO3)2 and K2Cr2O7 as Raman IFE references, the excitation and emission path lengths are found to exhibit chromophore and fluorophore independence, however path lengths are spectrofluorometer dependent. Finally, ratiometric resonance synchronous spectroscopy (R2S2) is discussed in Chapter IV. Using a combination of UV-vis and R2S2 spectroscopy, the optical cross sections of a wide range of nanomaterials were determined. Also on-resonance fluorescence in solution is demonstrated for the first time. The nanoparticles discussed range from photon absorbers, scatterers, simultaneous photon absorbers and scatterers, all the way to simultaneous photon absorbers, scatterers, and emitters.

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The purpose of this project is to explore the concept of inside-out change and inner sustainability in the context of societal norms through the process of how they change from childhood into adulthood. What are they and how do they affect our wellbeing and quality of life? To challenge the societal norms and become aware of its negative effects on the individual and collective, we need to allow ourselves space to acknowledge the importance of our inner life. The design aims to create a physical (and psychological) space with the help of video art and design, where the audience is given time for the mind to wander, to hopefully inspire and motivate for improved inner sustainability and change from the inside-out.

This thesis wants to save character and identity of local territory and bring a new function in to the historical objects. So it offers comfortable and special living and brings new values for the local inhabitants.

The aim of this master's thesis is the design of a poly-functional town house within a complicated topographic situation of Pekařská street's foothill defining its functional content following the neighborhood necessities analysis, mutual function relationship, construction and expression medium, by understanding organism of the city, its evolution, potentials and actual problems.

Transformation of educational space running in these days all over the world. Every one is conscious that our educational system is obsolete and we have to change it. Instead of designing of new school building o choose building which is running for education for almost one hundred and fifty years. One of these building is gymnasium in Hradec Kralove

Main aim of the thesis is to create an outdoor living space inside of tradition housing blocks. Principles of placemaking were used during a design process. One of the main ideas is to return a water to the urban courtyard according to its history.

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Research has demonstrated the importance of early social interactions in the development of self-talk. It does not appear, however, that existing research has examined the relationship between parents' self-talk and the self-talk that develops in their children. This study examined the relationship between self-talk in parents and their college-age children. Results revealed significant relationships between students' and parents' positive self-talk, but not negative self-talk. Marginal relationships were found for self-talk ratios (ratios of positive and negative self-talk). Maternal communication was found to mediate the relationship between students' and their mothers' positive self-talk. Different trends also were noted between genders. Finally, self-talk was related significantly to depression, anxiety, and self-esteem. Overall, results of this study emphasize the relationship between parents' and their children's positive self-talk and the importance of self-talk in psychological functioning. These findings lend promise to the possibility of modifying parents' self-talk and communication as a way to modify their children's self-talk and psychological functioning.