Bakalauro darbe analizuojami gimnazistų senelių išminties internalizacijos požymiai dorinio ugdymo kontekste. Tyrime dalyvavo 3 klasės Tauragės „Versmės“ gimnazijos 6 moksleiviai ir jų 6 seneliai. Tyrimu siekta išanalizuoti „N“ gimnazistų senelių išminties internalizacijos požymius dorinio ugdymo kontekste. Tyrimui atlikti iškelti uždaviniai. Išanalizuoti mokslinės literatūros šaltiniai apie išmintį, jos sampratą. Išanalizuoti mokslinė literatūros šaltiniai apie internalizacijos procesą ir jo ypatybes. Pasinaudojus literatūros šaltinių analizės duomenimis, sukonstruoti tyrimo instrumentą, gimnazistų senelių išminties internalizacijos požymiams tirti. Pasinaudojus klausimynu identifikuoti, gimnazistų bendravimo su seneliais patirties požymius. Išanalizavus apklausos rezultatus, identifikuoti senelių išminties internalizacijos požymius. / The features of internalization of the wisdom of the elderly of N gymnasium pupils in the context of moral training were analyzed in this bachelor work. Six students of “Versmės” gymnasium, of Tauragės district and their six grandparents participated in this investigation. The purpose of investigation was to find out the features of internalization of the wisdom of the elderly of N gymnasium pupils in the context of moral training The tasks were raised for study to perform. Sources of literature were analyzed about concept of wisdom. Sources of literature were analyzed about concept of internalization process and its peculiarities. Using analyzed the sources of literature, to construct research instrument, to find out the features of internalization of the wisdom of the elderly of N gymnasium pupils in the context of moral training.

Lactoferrin (Lf) is an iron binding protein produced in mammals. It has antimicrobial and immunomodulatory properties. Some bacteria that regularly colonize mammalian hosts have adapted to living in high Lf environments. Helicobacter pylori, which inhabits the human gut, was chosen as a model organism to investigate how bacteria may adapt to Lf. H. pylori was able to use iron from fully saturated human Lf (hLf) in various low iron media, achieving growth levels similar to the ironreplete control. Partially saturated hLf decreased growth, yet both partially saturated bovine Lf (bLf) and hLf were able to increase internalization of bacteria into mammalian tissue culture cells. A substantially larger increase in internalization was seen when bacteria were supplemented with hLf in low iron conditions, possibly mediated by iron-regulated cellular receptors or bacterial lactoferrin binding proteins. In eukaryotes, Lf is known to bind and facilitate internalization of DNA into cells and sometimes the nucleus, and upregulate gene expression. Here, one hundred bacterial genomes were surveyed for known Lf binding sites as an indication that Lf had similar functions using bacterial DNA. While the frequency and location of Lf binding sites suggest they occur at random, their presence in all genomes suggests that Lf may be able to act as a vector for bacterial DNA, and facilitate the movement of genes between species. Lf is being widely considered for commercial and therapeutic uses, with significant interest in producing it in genetically modified organisms (GMO). Widespread production and use of Lf could increase the number of bacteria that are adapted to it. How Lf interacts with bacteria adapted to it, and the ability of it to act as a DNA vector, may have relevance for GMO risk assessment.

This thesis is an effort to apply Elias’s thinking on social development to the Chinese social situation. At first glance his account of the civilizing process would appear incompatible with this context, in that, after state formation with the Qin and Han dynasties beginning in 221 BC, Chinese civilization remained both stable and highly traditional for well over two millennia. It is argued, however, that closer scrutiny reveals a process that was merely interrupted for a considerable period. The traditional system relied upon a symbiotic relationship between local society and the centre whereby the centre remained relatively small and aloof, not interfering with local social relations, as long as local society provided the required taxes and labour. In this situation the state had the monopolies of both violence and taxation that Elias would look for, but left local society to its own devices primarily because it was already pacified. This self-reinforcing system was enshrined and codified in the Confucian cannon over the course of centuries from the Han dynasty. Central control of the distribution of resources was eventually required to re-start the Chinese civilizing process, for this was the mechanism through which the local social structure would finally be altered. This only happened within the past century as the Chinese people struggled to grapple with their own ‘backwardness’ in the face of incessant Western and Japanese incursions. At this point the old system was toppled and replaced by progressively more aggressive central governments who saw as their most important task the destruction of the traditional social order in the interest of modernization. As the Chinese state consciously and forcibly took control of the distribution of resources at all levels of society, traditional social relations were stretched and warped, and the Chinese civilizing process re-commenced its long-stalled march toward modernization. This has been evidenced both by the dramatic growth in mobility and the rapidly extending chains of interdependence in the form of guanxi connections primarily during the Post-Opening period after 1978.

Lactoferrin (Lf) is an iron binding protein produced in mammals. It has antimicrobial and immunomodulatory properties. Some bacteria that regularly colonize mammalian hosts have adapted to living in high Lf environments. Helicobacter pylori, which inhabits the human gut, was chosen as a model organism to investigate how bacteria may adapt to Lf. H. pylori was able to use iron from fully saturated human Lf (hLf) in various low iron media, achieving growth levels similar to the ironreplete control. Partially saturated hLf decreased growth, yet both partially saturated bovine Lf (bLf) and hLf were able to increase internalization of bacteria into mammalian tissue culture cells. A substantially larger increase in internalization was seen when bacteria were supplemented with hLf in low iron conditions, possibly mediated by iron-regulated cellular receptors or bacterial lactoferrin binding proteins. In eukaryotes, Lf is known to bind and facilitate internalization of DNA into cells and sometimes the nucleus, and upregulate gene expression. Here, one hundred bacterial genomes were surveyed for known Lf binding sites as an indication that Lf had similar functions using bacterial DNA. While the frequency and location of Lf binding sites suggest they occur at random, their presence in all genomes suggests that Lf may be able to act as a vector for bacterial DNA, and facilitate the movement of genes between species. Lf is being widely considered for commercial and therapeutic uses, with significant interest in producing it in genetically modified organisms (GMO). Widespread production and use of Lf could increase the number of bacteria that are adapted to it. How Lf interacts with bacteria adapted to it, and the ability of it to act as a DNA vector, may have relevance for GMO risk assessment.

Thesis (M.A.)--University of Missouri-Columbia, 2007. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on January 3, 2008) Includes bibliographical references.

Thesis (Ph.D.)--University of Nebraska-Lincoln, 2009. / Title from title screen (site viewed February 25, 2010). PDF text: 140 p. : ill. ; 1 Mb. UMI publication number: AAT 3386561. Includes bibliographical references. Also available in microfilm and microfiche formats.

Thesis (Ph. D. in Psychology)--Vanderbilt University, Aug. 2005. / Title from title screen. Includes bibliographical references.

Mutual cell communication in the human body ensures the proper functioning of the essential mechanisms necessary for the life of the individual and preserving the homeostasis of the whole organism. Such communication is established by various types of signal transmission from the recipient cell to the donor cell, depending on the location and type of communicating cells. One such type is signalization through receptor molecules found on the surface or within the cell receiving the signal. These receptors receive the signal molecule in the form of a ligand and bind it to themselves, while activating the receptor and then triggering the intracellular signaling pathways. The most widely represented receptors in the eukaryotic organism include G-protein-coupled receptors, which represent signaling ensured by activation of the intracellular G-protein complex, and one of the main mechanisms occurring in neuronal signaling and signal transmission in the form of a neurotransmitter. Regulation of the amount of receptors on the surface of the cell and transport of the signal molecule into the intracellular spaces of the cell is ensured by the mechanism of endocytosis, whereby internalization of the ligand- bound receptor in the cytoplasm occurs. One of the most researched mechanisms is clatharin-mediated...

Nanostructures fabricated by different methods have become increasingly important for various applications at the cellular level. In order to understand how these nanostructures “behave” and for studying their internalization kinetics, several attempts have been made at tagging and investigating their interaction with living cells. In this study, magnetic iron nanowires with an iron oxide layer are coated with (3-Aminopropyl)triethoxysilane (APTES), and subsequently labeled with a fluorogenic pH-dependent dye pHrodo™ Red, covalently bound to the aminosilane surface. Time-lapse live imaging of human colon carcinoma HCT 116 cells interacting with the labeled iron nanowires is performed for 24 hours. As the pHrodo™ Red conjugated nanowires are non-fluorescent outside the cells but fluoresce brightly inside, internalized nanowires are distinguished from non-internalized ones and their behavior inside the cells can be tracked for the respective time length. A machine learning-based computational framework dedicated to automatic analysis of live cell imaging data, Cell Cognition, is adapted and used to classify cells with internalized and non-internalized nanowires and subsequently determine the uptake percentage by cells at different time points. An uptake of 85 % by HCT 116 cells is observed after 24 hours incubation at NW-to-cell ratios of 200. While the approach of using pHrodo™ Red for internalization studies is not novel in the literature, this study reports for the first time the utilization of a machine-learning based time-resolved automatic analysis pipeline for quantification of nanowire uptake by cells. This pipeline has also been used for comparison studies with nickel nanowires coated with APTES and labeled with pHrodo™ Red, and another cell line derived from the cervix carcinoma, HeLa. It has thus the potential to be used for studying the interaction of different types of nanostructures with potentially any live cell types.

No description available.