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An effective and rapid method for the separation of bacteria from food matrix remains a bottleneck for rapid bacteria detection for food safety. Bacteria can strongly attach to the food surface or internalize within the matrix which makes their isolation extremely difficult. Traditional methods of separating bacteria from foods routinely involve stomaching, blending and shaking, however these methods may not be efficient at removing all the bacteria from complex matrices. Here, we investigate the benefits of using enzyme digestion followed by immunomagnetic separation to isolate Salmonella from spinach and lettuce. Enzymatic digestion using pectinase and cellulase was able to break down the structure of the leafy green vegetables resulting in the detachment and release of Salmonella from the leaves. Immunomagnetic separation of Salmonella from the liquefied sample allowed an additional separation step to achieve a more pure sample without leaves debris that may benefit additional downstream applications. We have investigated the optimal combination of pectinase and cellulase for the digestion of spinach and lettuce to improve sample detection yields. The concentrations of enzymes used to digest the leaves were confirmed to have no significant effect on the viability of the inoculated Salmonella. Results reported that the recovery of the Salmonella from the produce after enzyme digestion of the leaves was significantly higher (P

Denna rapport behandlar en fallstudie i ett kandidatexamensarbete som utförts tillsammans med en livsmedelsbutik i Stockholmsområdet och som grundar sig i intervjuer, platsbesök och vetenskapliga artiklar. Där livsmedelsbutiken har ett intresse av att implementera en odling i form av ett hydroponiskt system direkt i sin butik.  I fallstudien har det undersökts hur implementering av odling i butik genom ett samarbete med ett odlingsföretag skulle fungera och se ut för butiken. Studien har begränsats till två olika odlingsföretag i Sverige, som har varsitt koncept på hur odlingen kan implementeras, gemensamt för dem är att de använder vertikal odling i form av hydroponiska system. Där det ena företaget erbjuder vertikal odling i en odlingscontainer och det andra vertikal odling inne i ett växthus. Båda företagens olika odlingskoncept har redan implementerats i två andra livsmedelsbutiker inom samma koncern som livsmedelsbutiken i Stockholm befinner sig i.  Fallstudien har genomförts genom att först skapa en bred bakgrund genom litteratursökningar i olika databaser kring relevanta nyckelord för att sedan genomföra intervjuer med båda odlingsföretagen och livsmedelsbutikerna. Därefter har kunskap och svar från respondenterna i intervjuerna sammanställts och ett förslag har tagits fram om vilket odlingsföretag som lämpar sig bäst för livsmedelsbutikens ändamål.  Resultatet visar att möjligheterna och fördelarna vid en implementering av hydroponisk odling för livsmedelsbutiken i Stockholmsområdet att implementera hydroponisk odling är flera och överväger till största del de möjliga utmaningarna. Dessutom gynnas flera av hållbarhetsmålen till livsmedelsbutikens koncern genom implementering av en hydroponisk odling i butiken. Vidare gynnas även några av de Förenta Nationernas Globala mål och även livsmedelsbutikens egna hållbarhetsmål. / This report is the result and outcome of a bachelor's thesis project conducted during the spring of 2021. The report presents the performed case study of a grocery store, in the area of Stockholm, where the company is aiming to implement a hydroponic self-cultivation inside their grocery store. The work is based on conducting interviews and reviewing established scientific articles in the field.  In the case study, it has been investigated how a potential collaboration between the grocery store and a cultivation company can be established. The study was limited to investigating two cultivation companies active on the Swedish market. These two cultivation companies have different solutions of how the cultivation can be implemented on the store area, but both offer vertical hydroponic solutions. Further, both systems of the individual cultivation companies’ have been implemented in other grocery stores that can be used as reference for validation of data.  The literature review of existing publications were conducted by searching in different databases by using the keywords of this work, for the researcher to increase knowledge to create guides for the interviews and for creating the theoretical frame of reference. Thereafter, literature findings and answers from the interviewees were compiled, analyzed and discussed to make a proposition of which cultivation company is best suited for a potential collaboration, with regard to the grocery stores’ wishes of implementing a hydroponic solution.  The results show that several of the grocery stores’ sustainability goals would benefit from a potential implementation of a self-cultivation. Also, implementing a hydroponic farm on the store area would increase the grocery store’s contribution towards achieving the Sustainable Development Goals.

This degree thesis investigates the possibilities of producing food inside a container at Svalbard, using renewable energy and energy storage. The idea was to be able to place the container at remote places without the need of being connected to the grid. We chose Svalbard, where it is cold and the sun is shining 24 hours a day at summertime. In the winter the opposite occurs and the sun is absent from the sky. The work is divided into theoretical studies and results based on different calculations. Such as economical evaluations (LCOE), and simulations using the computer programs Matlab and PVsyst. We have investigated if solar power and wind power is suitable as energy sources. Options for storage were batteries, grid and hydrogen storage. Different cases with Photovoltaics- and wind power plants, with batteries or grid, were compared against each other. It is not possible to use the grid as storage. This resulted in different sizing of our cases, with no excess energy production. The result showed that a 5 kWp photvoltaic plant with dual axis tracking system, was the most profitable. The Pay off would be 14 years and the total profit 63 453 SEK. If it will become possible in the future to use the grid at Svalbard as storage, it will open up opportunites for bigger systems. This will lead to higher profit than with smaller ones. Our results show that it is now most profitable with solar power.