Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal.

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.

Thesis (Ph.D.)--Boston University / PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you. / The problem of the dissertation is to discover what John Wesley meant by perfect love. Statements of both approbation and criticism regarding his doctrine are usually made from the vantage of various present-day interpretations. The goal of this study is to describe the type of perfection and love which was uppermost in Wesley's thought. The method used is motif analysis as it is developed by Anders Nygren in his book, Agape and Eros. Nygren's method and motifs (Agape, the New Testament motif; Eros, the Greek motif; Nomos, the Judaistic motif; and Caritas, Augustine's union of the Greek and New Testament motifs) are examined to determine their usefulness for research. While Nygren's description of Agape or New Testament love is not sufficiently complete, his description of the contrasting ways and systems of thought is sufficiently demonstrated to warrant the use of motif research . The method proved to be valuable in the examination of Wesley's thought [TRUNCATED] / 2031-01-01

This paper contains an analysis of the bronzed keys with bird motifs that were discovered during archaeological investigations between the years 1991 and 1997 at Kyrksundet, in the archipelago of southwestern Finland. Bronzed keys with bird motifs have never been found in Finland before, but similar keys have been found both in Birka and on Gotland, Sweden. The aim with this paper is firstly to analyse and compare the keys from Kyrksundet, Birka and Gotland, and their find contexts. Secondly, together with the results from the analysis, the following questions will be discussed; What is the meaning behind the bird motif, why can these keys be found at Kyrksundet, and who were the people that had these keys in their possession during the Viking Age. The symbolic aspect of the keys is a strong theme in this discussion, because the underlaying theory in this paper is that the keys most likely had both a worldly and a cosmological meaning. Earlier archaeological investigations mainly have associated these keys with the Nordic peoples and their eastern connections during the Viking Age. Reason behind this association is that the birds on the motif have been interpreted as falcons and the falcon has a relatively strong connection to the Rurik dynasty that ruled in Novgorod and Kiev. Whether the bird is a falcon or not, is however a question that will be discussed in this paper. The analysis performed in this paper, shows that the bird motif on the keys shares more similarities with a raven motif that was used on the British Isles than with the falcon motif that was used in Novgorod and Kiev. This paper will therefore include a suggestion for another perspective, where the keys might be connected to the Nordic peoples and their western connections.

CIAP1 (cellular Inhibitor of Apoptosis 1) possède une activité E3-ubiquitine ligase et présente des propriétés oncogéniques. Récemment, notre équipe a montré que cIAP1 pouvait réguler l’activité du facteur de transcription E2F1. L’objectif de mon travail de thèse était d’approfondir les mécanismes de cette régulation et d’évaluer l’importance de la coopération cIAP1-E2F1 dans l’activité oncogénique de cIAP1. J’ai démontré une interaction d’E2F1 avec la poche hydrophobe du domaine BIR3 de cIAP1. J’ai par ailleurs démontré l’importance de la première hélice α de ce domaine pour l’interaction de cIAP1 avec E2F1 et avec les autres protéines partenaires de cIAP1 capables de lier la poche hydrophobe du domaine BIR3. De plus, j’ai participé au travail montrant pour la première fois une régulation d’E2F1 par une ubiquitinylation non dégradative. cIAP1 permet la conjugaison de chaînes d’ubiquitines de type K63 sur les lysines 161 et 164 d’E2F1. Cette modification post-traductionnelle est indispensable à la stabilisation de la protéine lors d’un stress génotoxique et elle permet le recrutement du facteur de transcription sur les promoteurs des gènes cibles. Enfin, l’analyse des propriétés oncogéniques de cIAP1 n’ont pas permis, à ce jour, d’évaluer l’importance de la coopération cIAP1-E2F1. Cependant, nous avons montré l’importance du domaine BIR1 pour les propriétés oncogéniques de cIAP1 (domaine nécessaire à l’interaction de cIAP1 avec l’adaptateur moléculaire TRAF2). / The cellular inhibitor of Apoptosis 1 (cIAP1) behaves as an E3 ubiquitin ligase and has oncogenic properties. Previously, our team has shown that cIAP1 can regulate the E2F1 transcription factor activity. My research project has been focused on deepening our current knowledge on this interaction. Firstly, we characterized the E2F1-cIAP1 interaction, then we analyzed the regulation of E2F1 by cIAP1 and finally assessed the importance of the cIAP1-E2F1 interaction for the oncogenic properties of cIAP1. I have demonstrated a interaction of E2F1 with the hydrophobic pocket of the BIR3 domain of cIAP1. Moreover, I highlighted that the alpha 1 helix of the BIR3 domain is mandatory for the stability of this pocket. Moreover, we discovered an ubiquitination on lysine 161 and 164 of E2F1 by cIAP1. This ubiquitination is essential for the stability and transcriptional activity of E2F1. Finally, it appears that the cIAP1 BIR1 domain that is required for the interaction with TRAF2 is involved in its oncogenic properties.

Desenvolvemos aqui um estudo comparativo entre as epopéias gregas, Ilíada e Odisséia, e as indianas, Mahbharata e Ramayana. Analisamos, primeiramente, similitudes existentes entre essas obras. Nessa primeira etapa, mostramos que elas utilizam procedimentos literários semelhantes, têm os mesmos objetivos e apresentam aspectos mitológicos semelhantes: heróis descendentes de deuses, conformidade nas características dos deuses e temas análogos, além de detalhes análogos relativos ao gênero épico clássico, tais como o tempo pretérito arquetípico, a narração em terceira pessoa e estruturas discursivas semelhantes. Após o desenvolvimento dessa análise, voltamos nossa atenção para a atitude de súplica dos personagens, com o objetivo de demonstrar algumas diferenças significativas entre as atitudes de súplica exibidas pelos heróis gregos e pelos heróis indianos. Podemos observar em todas essas epopéias um quadro semelhante referente à atitude de súplica que busca restabelecer a ordem alterada: o herói ora ao seu deus para obter proteção e força que o tornem capaz de derrotar o inimigo, causador da desordem. Para além desse quadro, no entanto, com análise mais atenta, podemos verificar que o suplicante revela também intenções mais íntimas, ligadas aos anseios referentes à sua vida futura e cujo estudo nos leva a conhecer concepções teológicas e filosóficas da sociedade da época de produção dessas obras. Com o conhecimento das diferenças entre certos conceitos pertinentes à cultura grega e cultura indiana, obtemos elementos para a compreensão das diferenças entre as atitudes de súplica apresentadas pelos heróis dos épicos referentes a essas culturas. Assim, esse relacionamento entre deuses e homens é um aspecto fundamental nessas obras. Faz parte dos valores determinados de antemão pelo destinador sóciohistórico, com os quais o sujeito da enunciação constrói esses discursos. Nessas construções lingüísticas da Antigüidade há um contrato veridictório proposto e assumido referente à exposição alegórica do poder de atuação dos heróis. Nesse contrato, o enunciatário aceita a proposta de que esse poder é conferido pelos deuses. Desse modo, o sujeito da enunciação se desdobra em enunciador e enunciatário, cujas caracterizações nos permitem ter acesso à enunciação. O estudo das diferenças entre as atitudes de súplicas dos heróis gregos e dos heróis indianos foi levado a cabo através de um exame da construção dos sujeitos, em que analisamos marcas diversas existentes no texto - que nos permitem recuperar a enunciação - e também temas sustentados por formações ideológicas. Assim, após inserir cada texto no contexto de sua formação ideológica, examinamos o sentido construído pelo sujeito do discurso de cada obra, e procuramos mostrar essas diferenças / The aim of this thesis is to present a comparative study between the Greek epic poems The Iliad and The Odyssey, and the Indian epics The Mahabharata and The Ramayana. In a first instance we shall analyze some of the several similarities occurring within these epic poems. During this first stage, we shall highlight how these poems make use of similar literary elements; have similar objectives, present similar mythological aspects (i.e. heroes descending from gods; resemblances in the characteristics of the gods) and present similar themes. We also point out similarities in regard to the genre, such as the use of archetypal past tenses and of a third person narrative, as well as of similar discursive structures. After this first analysis we direct our attention to the pleading attitude of the characters in order to highlight some of the significant differences between the attitude of supplication exhibited by the Greek hero and the attitude of supplication exhibited by the Indian hero. Similar scenarios can be recognized in the body of all these epic poems in regard to this attitude of supplicating for the power to re-establish a harmony that has been disrupted: in all of them a hero prays to his god with the hope that he will be granted the protection and strength with which he will be able to defeat the cause of disturbance, depicted as the enemy. However when a more careful analysis is carried out we verify that the supplicant sometimes is also manifesting personal objectives, which are connected to his own expectations about his future. The study of these more subtle personal intentions took us to a thorough study of both the theological and philosophical thought processes of the Greek and Indian societies during the historical periods when these poems were produced. This study of the differences found within the above mentioned thought processes provided relevant information which in turn enabled us to better understand the subtle differences found in the pleading attitudes of the epic heroes related to these cultures. These relationships between gods and men are a fundamental aspect of these writings. They are directly linked to values previously established by the social and historical determiners, with which the subject of the enunciation builds up these discourses. In these linguistic structures of ancient times we find what can be referred to as a pre-established agreement between the enunciator and the enunciater. This agreement is directly connected to the allegorical exhibitions of the acting powers of the heroes, i.e. the enunciater accepts without questioning the proposal that the heroes\' superhuman powers are granted by the gods. Thus the subject of the enunciation unfolds itself between the enunciator and the enunciater, whose characterizations allow us to have access to the enunciation. In summary this study of the differences between the pleading attitude of the Greek hero and the Indian hero was undertaken through examining the construction of the subjective. We analysed several different elements of the texts - this allowed us to recover the enunciation - and we also analyzed themes supported by the ideological backgrounds of the texts. In this way, after inserting the text within the context of the specific historical ideological scenario of each one, we were able to examine the linguistic meanings brought up by the subjective of the discourses and subsequently seek to clarify the resulting differences

Cette thèse s'intéresse au problème de la recherche de motif dans les permutations, qui a pour objectif de savoir si un motif apparaît dans un texte, en prenant en compte que le motif et le texte sont des permutations. C'est-à-dire s'il existe des éléments du texte tel que ces éléments sont triés de la même manière et apparaissent dans le même ordre que les éléments du motif. Ce problème est NP complet. Cette thèse expose des cas particuliers de ce problème qui sont solvable en temps polynomial.Pour cela nous étudions le problème en donnant des contraintes sur le texte et/ou le motif. En particulier, le cas où le texte et/ou le motif sont des permutations qui ne contiennent pas les motifs 2413 et 3142 (appelé permutation séparable) et le cas où le texte et/ou le motif sont des permutations qui ne contiennent pas les motifs 213 et 231 sont considérés. Des problèmes dérivés de la recherche de motif et le problème de la recherche de motif bivinculaire sont aussi étudiés. / This thesis focuses on permutation pattern matching problem, which askswhether a pattern occurs in a text where both the pattern and text are permutations.In other words, we seek to determine whether there exist elements ofthe text such that they are sorted and appear in the same order as the elementsof the pattern. The problem is NP-complete. This thesis examines particularcases of the problem that are polynomial-time solvable.For this purpose, we study the problem by giving constraints on the permutationstext and/or pattern. In particular, the cases in which the text and/orpattern are permutations in which the patterns 2413 and 3142 do not occur(also known as separable permutations) and in which the text and/or patternare permutations in which the patterns 213 and 231 do not occur (also known aswedge permutations) are also considered. Some problems related to the patternmatching and the permutation pattern matching with bivincular pattern arealso studied.

Desenvolvemos aqui um estudo comparativo entre as epopéias gregas, Ilíada e Odisséia, e as indianas, Mahbharata e Ramayana. Analisamos, primeiramente, similitudes existentes entre essas obras. Nessa primeira etapa, mostramos que elas utilizam procedimentos literários semelhantes, têm os mesmos objetivos e apresentam aspectos mitológicos semelhantes: heróis descendentes de deuses, conformidade nas características dos deuses e temas análogos, além de detalhes análogos relativos ao gênero épico clássico, tais como o tempo pretérito arquetípico, a narração em terceira pessoa e estruturas discursivas semelhantes. Após o desenvolvimento dessa análise, voltamos nossa atenção para a atitude de súplica dos personagens, com o objetivo de demonstrar algumas diferenças significativas entre as atitudes de súplica exibidas pelos heróis gregos e pelos heróis indianos. Podemos observar em todas essas epopéias um quadro semelhante referente à atitude de súplica que busca restabelecer a ordem alterada: o herói ora ao seu deus para obter proteção e força que o tornem capaz de derrotar o inimigo, causador da desordem. Para além desse quadro, no entanto, com análise mais atenta, podemos verificar que o suplicante revela também intenções mais íntimas, ligadas aos anseios referentes à sua vida futura e cujo estudo nos leva a conhecer concepções teológicas e filosóficas da sociedade da época de produção dessas obras. Com o conhecimento das diferenças entre certos conceitos pertinentes à cultura grega e cultura indiana, obtemos elementos para a compreensão das diferenças entre as atitudes de súplica apresentadas pelos heróis dos épicos referentes a essas culturas. Assim, esse relacionamento entre deuses e homens é um aspecto fundamental nessas obras. Faz parte dos valores determinados de antemão pelo destinador sóciohistórico, com os quais o sujeito da enunciação constrói esses discursos. Nessas construções lingüísticas da Antigüidade há um contrato veridictório proposto e assumido referente à exposição alegórica do poder de atuação dos heróis. Nesse contrato, o enunciatário aceita a proposta de que esse poder é conferido pelos deuses. Desse modo, o sujeito da enunciação se desdobra em enunciador e enunciatário, cujas caracterizações nos permitem ter acesso à enunciação. O estudo das diferenças entre as atitudes de súplicas dos heróis gregos e dos heróis indianos foi levado a cabo através de um exame da construção dos sujeitos, em que analisamos marcas diversas existentes no texto - que nos permitem recuperar a enunciação - e também temas sustentados por formações ideológicas. Assim, após inserir cada texto no contexto de sua formação ideológica, examinamos o sentido construído pelo sujeito do discurso de cada obra, e procuramos mostrar essas diferenças / The aim of this thesis is to present a comparative study between the Greek epic poems The Iliad and The Odyssey, and the Indian epics The Mahabharata and The Ramayana. In a first instance we shall analyze some of the several similarities occurring within these epic poems. During this first stage, we shall highlight how these poems make use of similar literary elements; have similar objectives, present similar mythological aspects (i.e. heroes descending from gods; resemblances in the characteristics of the gods) and present similar themes. We also point out similarities in regard to the genre, such as the use of archetypal past tenses and of a third person narrative, as well as of similar discursive structures. After this first analysis we direct our attention to the pleading attitude of the characters in order to highlight some of the significant differences between the attitude of supplication exhibited by the Greek hero and the attitude of supplication exhibited by the Indian hero. Similar scenarios can be recognized in the body of all these epic poems in regard to this attitude of supplicating for the power to re-establish a harmony that has been disrupted: in all of them a hero prays to his god with the hope that he will be granted the protection and strength with which he will be able to defeat the cause of disturbance, depicted as the enemy. However when a more careful analysis is carried out we verify that the supplicant sometimes is also manifesting personal objectives, which are connected to his own expectations about his future. The study of these more subtle personal intentions took us to a thorough study of both the theological and philosophical thought processes of the Greek and Indian societies during the historical periods when these poems were produced. This study of the differences found within the above mentioned thought processes provided relevant information which in turn enabled us to better understand the subtle differences found in the pleading attitudes of the epic heroes related to these cultures. These relationships between gods and men are a fundamental aspect of these writings. They are directly linked to values previously established by the social and historical determiners, with which the subject of the enunciation builds up these discourses. In these linguistic structures of ancient times we find what can be referred to as a pre-established agreement between the enunciator and the enunciater. This agreement is directly connected to the allegorical exhibitions of the acting powers of the heroes, i.e. the enunciater accepts without questioning the proposal that the heroes\' superhuman powers are granted by the gods. Thus the subject of the enunciation unfolds itself between the enunciator and the enunciater, whose characterizations allow us to have access to the enunciation. In summary this study of the differences between the pleading attitude of the Greek hero and the Indian hero was undertaken through examining the construction of the subjective. We analysed several different elements of the texts - this allowed us to recover the enunciation - and we also analyzed themes supported by the ideological backgrounds of the texts. In this way, after inserting the text within the context of the specific historical ideological scenario of each one, we were able to examine the linguistic meanings brought up by the subjective of the discourses and subsequently seek to clarify the resulting differences

L'arrivée des technologies de séquençage d’ADN à haut-débit a représenté une révolution dans le domaine de la génomique personnalisée, en raison de leur résolution et leur faible coût. Toutefois, ces nouvelles technologies présentent un taux d’erreur élevé, qui varie entre 0,1% et 1% pour les séquenceurs de seconde génération. Cette valeur est problématique dans le cadre de la recherche de variants de faible ratio allélique, comme ce qui est observé dans le cas des tumeurs hétérogènes. En effet, un tel taux d’erreur peut mener à des milliers de faux positifs. Chaque région de l’ADN étudié doit donc être séquencée plusieurs fois, et les variants sont alors filtrés en fonction de critères basés sur leur profondeur. Malgré ces filtres, le nombre d’artefacts reste important, montrant la limite des approches conventionnelles et indiquant que certains artefacts de séquençage ne sont pas aléatoires.Dans le cadre de cette thèse, nous avons développé un algorithme exact de recherche des motifs d’ADN dégénérés sur-représentés en amont des erreurs de séquençage non aléatoires et donc potentiellement liés à leur apparition. Cet algorithme a été mis en oeuvre dans un logiciel appelé DiNAMO, qui a été testé sur des données de séquençage issues des technologies IonTorrent et Illumina.Les résultats expérimentaux ont mis en évidence plusieurs motifs, spécifiques à chacune de ces deux technologies. Nous avons ensuite montré que la prise en compte de ces motifs dans l’analyse, réduisait considérablement le taux de faux positifs. DiNAMO peut donc être utilisé en aval de chaque analyse, comme un filtre supplémentaire permettant d’améliorer l’identification des variants, en particulier des variants à faible ratio allélique. / The advent of Next Generation DNA Sequencing technologies has revolutionized the field of personalized genomics through their resolution and low cost. However, these new technologies are associated with a relatively high error rate, which varies between 0.1% and 1% for second-generation sequencers. This value is problematic when searching for low allelic ratio variants, as observed in the case of heterogeneous tumors. Indeed, such error rate can lead to thousands of false positives. Each region of the studied DNA must therefore be sequenced several times, and the variants are then filtered according to criteria based on their depth. Despite these filters, the number of errors remains significant, showing the limit of conventional approaches and indicating that some sequencing errors are not random.In the context of this thesis, we have developed an exact algorithm for over-represented degenerate DNA motifs discovery on the upstream of non-random sequencing errors and thus potentially linked to their appearance. This algorithm was implemented in a software called DiNAMO, which was tested on sequencing data from IonTorrent and Illumina technologies.The experimental results revealed several motifs, specific to each of these two technologies. We then showed that taking these motifs into account in the analysis reduced significantly the false-positive rate. DiNAMO can therefore be used downstream of each analysis, as an additional filter to improve the identification of variants, especially, variants with low allelic ratio.