Orientador: Helena Maria Andre Bolini / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos / Made available in DSpace on 2018-08-11T04:55:59Z (GMT). No. of bitstreams: 1 Alves_LeonardoRangel_M.pdf: 410164 bytes, checksum: eed7ffe76f347f00d0abb009ed908230 (MD5) Previous issue date: 2008 / Resumo: Este estudo objetivou Identificar direcionadores de preferência de oito amostras comerciais de néctar de uva (tradicionais e ¿light¿) utilizando metodologias estatísticas avançadas para relacionar dados de perfil sensorial, físico-químicos e aceitabilidade. Oito amostras comerciais de néctares de uva (quatro tradicionais e suas respectivas versões ¿light¿) foram analisadas. Um teste de Aceitação utilizando a escala hedônica híbrida foi realizado com 114 consumidores. Quatorze termos descritivos foram avaliados por uma equipe sensorial e seis atributos físico-químicos foram medidos. As amostras de néctar de uva A e C foram as mais aceitas e as amostras CL e DL (¿light¿) foram as mais rejeitadas. Construiu-se um Mapa de Preferência Interno e em seguida uma Análise de ¿Cluster¿ foi realizada para o atributo Impressão Global. Dois grupos de consumidores foram encontrados. A principal diferença entre os grupos foi com relação à utilização de diferentes porções da escala pelos consumidores de cada grupo. A metodologia PLSR foi utilizada para relacionar a aceitação dos consumidores com os termos descritivos e atributos físico-químicos, fornecendo correlações entre eles. Os resultados mostraram que os atributos Sabor de Uva, Sabor Residual de Uva, Acidez Total Titulável, Aroma de Uva, Cor Vinho, °Brix, Viscosidade, Acidez, Turbidez, Adstringência, Fenóis Totais e Consistência nesta ordem de importância, estavam fortemente correlacionados com a Impressão Global dos consumidores sendo portanto os direcionadores de preferência encontrados / Abstract: This study depicts the PLS regression method used to help find drivers of liking of the grape nectar. Eight commercial brands (four traditional and four lights) were analyzed. An acceptance test using hybrid hedonic scale was performed with 114 consumers. Fourteen attributes were evaluated by a sensory team of fourteen members, and six physical-chemical attributes were measured. The most accepted samples were A and C, and the less accepted ones were CL and DL (lights). An Internal Preference Mapping followed by a Cluster Analysis was performed on the consumer grades to Global Impression. Two clusters of consumers were found. The mainly difference between clusters was the use of different portions of the scale by the consumers. The PLSR methodology was used to relate the acceptance with the sensory and physical-chemical attributes giving a correlation between them. The model showed the importance of each sensory or physicalchemical attribute for the model projection. The results showed that Grape Flavor; Residual Grape Flavor, Total Sourness Titration, Grape Aroma, Wine Color, °Brix, Viscosity, Sourness, Turbidity, Astringency, Total Phenols and Consistency were positive correlated with consumer grades to Global Impression, therefore they are called drivers of liking / Mestrado / Consumo e Qualidade de Alimentos / Mestre em Alimentos e Nutrição

A Mata Atlântica é considerada uma das grandes prioridades para a conservação de biodiversidade em todo o continente americano. Este bioma é um dos oito hotspots do planeta e caracteriza-se pelo alto grau de endemismo e pela alta diversidade de espécies que encerra. As abelhas Euglossini estão restritas à região Neotropical, com uma faixa de distribuição que se estende desde o Norte do México até a província de Córdoba, na Argentina. As espécies dessa tribo são abundantes em florestas tropicais úmidas/chuvosas e florestas subtropicais da América Central e América do Sul onde ocorrem em maior diversidade. Em virtude do alto impacto causado pelas ações humanas em áreas de Mata Atlântica e o pouco que se conhece sobre sua diversidade, este trabalho propõe um estudo geral sobre a estrutura das comunidades de espécies de Euglossini presentes em uma área de planície litorânea continental, Parque Estadual da Serra do Mar - Núcleo Picinguaba (PESM), e uma área insular, Parque Estadual da Ilha Anchieta (PEIA), no município de Ubatuba, norte de São Paulo, Brasil. O PESM abrange uma área de aproximadamente 47.500 hectares e quase todos os ecossitemas da Mata Atlântica são encontrados em seus domínios. O PEIA cobre toda a extensão da Ilha Anchieta (828 hectares), distante cerca de 600 m do continente. As coletas foram realizadas mensalmente, de Agosto/2007 a Julho/2009, com o uso de 14 compostos aromáticos utilizados como iscas artificiais para a atração de machos, além de indivíduos coletados em flores. Mil quinhentos e setenta e seis espécimes (73 fêmeas e 1503 machos), pertencentes a 24 espécies, foram registrados no presente trabalho. A espécie mais abundante foi Euglossa cordata (36,4%), seguida por Euglossa iopoecila (13,9%) e Euglossa sapphirina (13,3%). Dezoito espécies foram amostradas na Ilha Anchieta, sendo que quatro delas foram exclusivas desta área, ao passo que 20 espécies foram amostradas no PESM e seis delas foram registradas apenas neste local. Na Ilha Anchieta, Eg. cordata representou 63,2%, quase dois terços do total de espécies coletadas. Já em Picinguaba, Eg. iopoecila (23,0%) e Eg. sapphirina (21,0%) foram as duas espécies dominantes. A espécie Eg. iopoecila, a mais abundante na área continental, não foi registrada na llha e Eg. sapphirina, a segunda espécie mais frequente no PESM, foi representada apenas por nove indivíduos na Ilha Anchieta e por 200 em Picinguaba. Estes dados sugerem que essas duas espécies podem atuar como bioindicadores de ambientes preservados, como sugerido para outras espécies de Euglossini. Por outro lado, alguns autores evidenciaram que Eg. cordata é uma espécie favorecida por ambientes perturbados, o que pode estar associado à sua alta abundância na Ilha Anchieta. Dados de literatura mostram que há um sinal evidente de perturbação ambiental, além da perda de espécies, é a dominância de algumas espécies. A Ilha Anchieta apresenta um longo histórico de ocupação humana e diversas espécies de plantas e animais foram introduzidos em seus domínios. / The Atlantic Forest is considered as one of the highest priorities for the conservation of biodiversity in the American continent. This biome is one of the eight biodiversity hotspots in the world and is characterized by high species diversity and endemism. Euglossini bees are restricted to the Neotropics, with a geographic range that extends from northern Mexico to the Córdoba Province in Argentine. Species from this tribe are abundant in humid and rainy tropical forests and in sub-tropical forests of Central and South America, where their diversity is high. Due to the high impact caused by human activities in the Atlantic Forest and the poor knowledge on its biodiversity, in the present study we assessed the community structure of the Euglossini in a coastal lowland area: Parque Estadual da Serra do Mar - Núcleo Picinguaba (PESM); and in an insular area: Parque Estadual da Ilha Anchieta (PEIA), Ubatuba, both located in northern São Paulo State, Brazil. PESM has an area of approximately 47.500 ha and almost all ecosystems of the Atlantic Forest are found within its domains. PEIA covers all the extension of the Anchieta Island (828 ha), about 600 m away from the continent. Sampling was carried out monthly, from August 2007 to July 2009, using artificial baits with 14 aromatic compounds to attract males, as well as by collecting individuals on flowers. One thousand five hundred and seventy-six specimens (73 females and 1,503 males), represented by 24 species were recorded. The most abundant species was Euglossa cordata (36.4%), followed by Euglossa iopoecila (13.9%) and Euglossa sapphirina (13.3%). Eighteen species were sampled on Anchieta Island and four of them were exclusive to this area; whereas 20 species were sampled in PESM and six of them were only recorded there. On Anchieta Island, E. cordata represented almost two thirds of the total species collected (63.2%). In Picinguaba, E. iopoecila (23.0%) and E. sapphirina (21.0%) were the dominant species. E. iopoecila, the most abundant species in the continental area, was not recorded on the island, and E. sapphirina, the second most frequent species in PESM, was represented only by nine individuals on Anchieta Island and by 200 in Picinguaba. Results suggest that these two species may act as bioindicators of preserved environments, as suggested for other Euglossini species. However, some authors evidenced that E. cordata is favored by disturbed environments, which may be associated with its high abundance on Anchieta Island. Other studies show that an evident sign of environmental perturbation, besides species loss, is the dominance of some species. Anchieta Island exhibits a long history of human occupation and several plant and animal species were introduced there.

Les insectes contribuent à la pollinisation de 70% des espèces cultivées aujourd’hui à travers le monde. Avec l’intensification de l’agriculture au début du XXème siècle, les agriculteurs se sont mis à introduire des colonies d’abeilles mellifères dans leurs cultures entomophiles pour assurer le service de pollinisation. Avec la reconnaissance croissante du rôle majeur joué par les insectes sauvages dans la pollinisation des cultures, le concept de pollinisation intégrée des cultures a récemment vu le jour, encourageant à combiner insectes pollinisateurs introduits et sauvages en adaptant les pratiques agricoles pour assurer une pollinisation durable des cultures. Mais l’introduction d’insectes pollinisateurs d’élevage est une pratique encore très empirique qui manque de références techniques pour pouvoir être mise en œuvre avec précision. L’objectif général de cette thèse était de fournir des éléments pour mettre au point une méthode objective pour définir la charge en unités opérationnelles d’abeilles mellifères à introduire par unité de surface de culture cible pour complémenter la faune pollinisatrice sauvage pour que la pollinisation ne soit pas un facteur de production limitant.Nous avons travaillé sur une lignée mâle fertile et une lignée mâle stérile de colza (Brassica napus L.), les productions de semence hybride dépendant entièrement des insectes pour la pollinisation chez cette espèce. Nous avons pu déterminer (i) le nombre minimum de grains de pollen viables devant être déposés par stigmate pour que la grenaison puisse être complète en fonction de la température, (ii) la durée après anthèse pendant laquelle le pollen doit être déposé sur le stigmate pour que la grenaison puisse être complète en fonction de la température, (iii) la durée pendant laquelle une fleur sécrète du nectar en fonction de la température pour chacune des deux lignées, et (iv) la vitesse de cette sécrétion nectarifère en fonction de la température pour chacune des deux lignées. Nous avons également validé une méthode d’évaluation rapide de la taille des cheptels d’abeilles mellifères introduits dans les cultures entomophiles, couramment utilisée dans certains pays, en tenant compte de la température.A partir de ces éléments, nous avons proposé d’introduire les concepts d’offre et de demande en pollinisation d’une culture cible, afin de quantifier les déficits de pollinisation et le nombre d’insectes pollinisateurs à introduire pour compléter ces déficits. Ces premiers éléments fournissent une base pour construire un modèle mécaniste de gestion intégrée de la pollinisation, pour prédire le nombre d’insectes pollinisateurs à introduire dans une culture étant donné son contexte climatique, paysager et variétal. / Insects contribute to the pollination of 70% of the species cultivated today around the world. With the agriculture intensification in the early twentieth century, farmers began to introduce honey bees colonies into their entomophilous crops to provide pollination service. With the growing recognition of the major role played by wild insects in crop pollination, the concept of integrated crop pollination recently emerged, encouraging the combination of introduced and wild pollinating insects by adapting agricultural practices to ensure sustainable pollination of crops. But the introduction of managed pollinating insects is still an empirical practice that lacks technical references to be implemented with precision. The general objective of this thesis was to provide elements to develop an objective method to define the operational unit load of honey bees to be introduced per unit area of target crop to complement wild pollinating fauna so that pollination is not a limiting factor for production.We worked on a male fertile and a male sterile oilseed rape (Brassica napus L.) lines, hybrid seed productions being entirely dependent on insects for pollination in this species. We were able to determine (i) the minimum number of viable pollen grains to be deposited on stigma so that seed set was complete depending on the temperature, (ii) the duration after anthesis during which the pollen must be deposited on the stigma so that seed set was complete depending on the temperature, (iii) the length of time a flower secretes nectar as a function of temperature for each of the two lines, and (iv) the rate of this nectar secretion as a function of temperature for each of the two lines. We also validated a method for rapid assessment of the size of honey bee stocking rate introduced into entomophilous crops, commonly used in some countries, taking into account temperature.From these elements, we proposed to introduce the concepts of supply and demand in pollination of a target crop, in order to quantify the pollination deficits and the number of pollinating insects to introduce to complete these deficits. These first elements provide a basis for constructing a mechanistic model of integrated pollination management, to predict the number of pollinating insects to be introduced into a crop given its climatic, landscape and varietal context.

The goals of this research project were to identify the various insects observed to visit inflorescences of Echinacea angustifolia DC, and to rank these visitors according to their importance as pollinators of E. angustifolia in Saskatchewan. Studying nectar and the nectary is essential to understanding the interaction of disc florets with pollinators. Nectar-sugar production by disc florets of E. angustifolia and E. purpurea (L. Moench) was quantified from anthesis to cessation with production per disc floret peaking in the afternoon of the staminate phase (191.7 µg) and at midday of the first day of the pistillate phase (156.6 µg), respectively. Morphology of the disc-like floral nectaries of both Echinacea species was studied, as well as the ultrastructure of the nectary of E. purpurea. Modified stomata on the nectary rim are the most likely exits for nectar, but creases in the epidermis may also participate. The nectary of E. purpurea is vascularized by phloem alone, which occurred adjacent to the epidermis. Companion cells possessed wall ingrowths, and these cells may unload arriving sugar destined for either an apoplastic or symplastic pathway. Lobed nuclei were a key feature of secretory parenchyma cells, as was a predominance of mitochondria, suggesting that energy-requiring eccrine secretion predominates in E. purpurea. E. angustifolia exhibited a generalist pollination system, with pollinating insects belonging to the orders Coleoptera, Diptera, Hymenoptera, and Lepidoptera. The pollination efficiency of visitors was determined by single insect visits to bagged, virgin inflorescences followed by quantifying pollen tubes at the bases of receptive styles and/or calculating the percentage of shrivelled styles. It was determined that bumble bees (Bombus spp.) were efficient pollinators, indicating that they would likely contribute much to the pollination of E. angustifolia. Grasshopper bee flies (Systoechus vulgaris Loew) were plentiful but individually were not efficient pollinators, but taken together, they provided much pollination. Golden blister beetles (Epicauta ferruginea Say) were efficient pollinators but where yellow-petalled flowers occurred, their numbers on E. angustifolia decreased. Honey bees (Apis mellifera L.) were efficient pollinators and were present in low numbers without managed introduction. Pierid (2003) butterflies were regular visitors and efficient pollinators, and likely contributed significantly to E. angustifolia pollination. When introduced, the alfalfa leafcutter bee (Megachile rotundata Fabr.) preferred not to forage on E. angustifolia and as such, these solitary bees were not suitable as managed pollinators. In large agricultural plantings of E. angustifolia, however, native insects may not be capable of providing sufficient pollination for seed production when floral competition occurs.

The goals of this research project were to identify the various insects observed to visit inflorescences of Echinacea angustifolia DC, and to rank these visitors according to their importance as pollinators of E. angustifolia in Saskatchewan. Studying nectar and the nectary is essential to understanding the interaction of disc florets with pollinators. Nectar-sugar production by disc florets of E. angustifolia and E. purpurea (L. Moench) was quantified from anthesis to cessation with production per disc floret peaking in the afternoon of the staminate phase (191.7 µg) and at midday of the first day of the pistillate phase (156.6 µg), respectively. Morphology of the disc-like floral nectaries of both Echinacea species was studied, as well as the ultrastructure of the nectary of E. purpurea. Modified stomata on the nectary rim are the most likely exits for nectar, but creases in the epidermis may also participate. The nectary of E. purpurea is vascularized by phloem alone, which occurred adjacent to the epidermis. Companion cells possessed wall ingrowths, and these cells may unload arriving sugar destined for either an apoplastic or symplastic pathway. Lobed nuclei were a key feature of secretory parenchyma cells, as was a predominance of mitochondria, suggesting that energy-requiring eccrine secretion predominates in E. purpurea. E. angustifolia exhibited a generalist pollination system, with pollinating insects belonging to the orders Coleoptera, Diptera, Hymenoptera, and Lepidoptera. The pollination efficiency of visitors was determined by single insect visits to bagged, virgin inflorescences followed by quantifying pollen tubes at the bases of receptive styles and/or calculating the percentage of shrivelled styles. It was determined that bumble bees (Bombus spp.) were efficient pollinators, indicating that they would likely contribute much to the pollination of E. angustifolia. Grasshopper bee flies (Systoechus vulgaris Loew) were plentiful but individually were not efficient pollinators, but taken together, they provided much pollination. Golden blister beetles (Epicauta ferruginea Say) were efficient pollinators but where yellow-petalled flowers occurred, their numbers on E. angustifolia decreased. Honey bees (Apis mellifera L.) were efficient pollinators and were present in low numbers without managed introduction. Pierid (2003) butterflies were regular visitors and efficient pollinators, and likely contributed significantly to E. angustifolia pollination. When introduced, the alfalfa leafcutter bee (Megachile rotundata Fabr.) preferred not to forage on E. angustifolia and as such, these solitary bees were not suitable as managed pollinators. In large agricultural plantings of E. angustifolia, however, native insects may not be capable of providing sufficient pollination for seed production when floral competition occurs.

In this study, buckwheat (Fagopyrum esculentum Moench) and alyssum (Lobularia maritima (L.)) flowers were used to examine the effect of floral resources on the efficacy of the leafroller parasitoid Dolichogenidea tasmanica (Cameron) in vineyards. This was done by assessing the influence of these flowers on parasitoid abundance and parasitism rate, and by investigating the consequences of this for leafroller abundance. In laboratory experiments, alyssum flowers were used to investigate the effect of floral food on the longevity, fecundity and sex ratio of D. tasmanica. Dolichogenidea tasmanica comprised more than 95 % of parasitoids reared from field collected leafrollers in this study. The abundance of D. tasmanica during the 1999-2000 growing season was very low compared with previous studies, possibly due to the very low abundance of its leafroller hosts during the experiment. The number of males of this species on yellow sticky traps was increased (although not significantly) when buckwheat flowers were planted in a Marlborough vineyard; however, the number of female D. tasmanica on traps was no greater with flowers than without. The abundance of another leafroller parasitoid, Glyptapanteles demeter (Wilkinson)(Hymenoptera: Braconidae), on traps was also not significantly affected by the presence of buckwheat flowers, although females of this species were caught in greater numbers in the control than in buckwheat plots. Naturally-occurring leafrollers were collected from three vineyard sites in Marlborough, and one in Canterbury during the 2000-2001 season to assess the effect of buckwheat and alyssum flowers on parasitism rate. Parasitism rate more than doubled in the presence of buckwheat at one of the Marlborough vineyards, but alyssum had no effect on parasitism rate in Canterbury. A leafroller release/recover method, used when naturally-occurring leafrollers were too scarce to collect, was unable to detect any effect of buckwheat or alyssum on parasitism rate. Mean parasitism rates of approximately 20 % were common in Marlborough, although rates ranged from 0 % to 45 % across the three vineyard sites in that region. In Canterbury in April, mean parasitism rates were approximately 40 % (Chapter 4). Rates were higher on upper canopy leaves (40-60 %) compared with lower canopy leaves and bunches (0-25 %). Leafroller abundance was apparently not affected by the presence of buckwheat in Marlborough, or alyssum in Canterbury. Buckwheat did, however, significantly reduce the amount of leafroller evidence (webbed leafroller feeding sites on leaves or in bunches) in Marlborough, suggesting that the presence of these flowers may reduce leafroller populations. Leafrollers infested less than 0.1 % of Cabernet Sauvignon leaves throughout the 1999-2000 growing season, but increased in abundance in bunches to infest a maximum of 0.5 % of bunches in late March in Marlborough. In Pinot Noir vines in the 2000-2001 season, leafroller abundance was also low, although sampling was not conducted late in the season when abundance reaches a peak. In Riesling vines in Canterbury, between 1.5 % and 2.5 % of bunches were infested with leafrollers in April. In the laboratory, alyssum flowers significantly increased the longevity and lifetime fecundity of D. tasmanica compared with a no-flower treatment. However, daily fecundity was not increased by the availability of food, suggesting that the greater lifetime fecundity was related to increases in longevity. Parasitoids were also able to obtain nutrients from whitefly honeydew, which resulted in similar longevity and daily fecundity to those when alyssum flowers were present. The availability of food had a significant effect on the offspring sex ratio of D. tasmanica. Parasitoids reared from naturally-occurring leafrollers produced an equal sex ratio, assumed to be the evolutionarily stable strategy (ESS) for this species. In the laboratory, this ESS was observed only when parasitoids had access to alyssum flowers. Without food, or with honeydew only, sex ratios were strongly male-biased. In the field, floral resources affected the sex ratio of D. tasmanica only when this species was reared from leafrollers released and recovered in Marlborough. In that experiment, buckwheat shifted the sex ratio in favour of female production from the equal sex ratio found in control plots. No firm explanations can be given to account for these results, due to a lack of research in this area. Possible mechanisms for the changes in sex ratio with flowers are discussed. This study demonstrated that flowers are an important source of nutrients for D. tasmanica, influencing the longevity, fecundity and offspring sex ratio of this species. However, only some of the field experiments were able to show any positive effect of the provision of floral resources on parasitoid abundance or parasitism rate. More information is needed on the role these parasitoids, and other natural enemies, play in regulating leafroller populations in New Zealand vineyards, and on how they use floral resources in the field, before recommendations can be made regarding the adoption of this technology by growers.