The Swedish debate regarding the reform of the third person pronoun plural has been examined with the NLS theory and with the perspective of literacy as not only a mental process but also as a social practice. The debate was initiated in autumn of 2016 by an article written by the teacher Henrik Birkebo in which he argued for a reform. To examine the legitimacy of the main arguments in the debate in relation to pupils’ actual use of the Swedish alternatives for the third person pronoun plural de/dem and dom in secondary school, 307 answers to the National Tests in Swedish and Swedish as a Second Language from 2015 has been quantified in a corpus. The result shows that the argument for a reform due to the hardship for pupils to make out the difference between de and dem sees literacy mainly as a mental process. This is not shown in the corpus. A minority cannot use de and dem as the norm requires, and this minority consists of pupils with high and low grades. The argument to not too hastily make a reform sees literacy as a social practice and as a performance in a social context. This is more likely to be true according to the results of the analysis of the corpus. 82 % of the pupils want to use de/dem which shows that this is seen as a more respected literacy than the use of dom, which only 6 % of the pupils use in the corpus.

Hepatitis B Virus (HBV) Infektionen verursachen entzündliche Erkrankungen der Leber. Insbesondere die frühen Phasen des HBV Lebenszyklus sind noch nicht geklärt, so ist z.B. der Rezeptorkomplex an den HBV bindet unbekannt. Mittlerweile stehen neue Infektionsmodelle zur Verfügung um den HBV Lebenszyklus zu untersuchen. Dies erfordert eine effiziente Zellkultur basierende Methode um große Mengen infektiöser Partikel zu generieren. Ein Ziel der Arbeit war durch Kultivierung auf Mikrocarrier die HBV Produktion der Zelllinie HepG2.2.15 zu steigern. Die Analyse von Protein und HBV Sekretion, Infektiösität und MAP Signalübertragung ergab eine 18x höhere HBV Produktion bei einer reduzierten Sekretion von subviralen Partikeln durch HepG2.2.15 die auf Mikrocarrier kultiviert wurden. Der Anstieg der Virusproduktion korreliert mit einer verstärkten Aktivierung der MAP Kinase ERK-2, die mit HBV Replikation in Verbindung steht. Ein weiterer wenig verstandener Teil des HBV Lebenszyklus ist der Kernimport des HBV Genoms. Spuren der viralen Polymerase finden sich im Zellkern von HBV infizierten Zellen. Ziel der Arbeit war, Motive in der HBV Polymerase zu finden, die in der Lage sind Zelllokalisation zu beeinflussen. Durch Sequenzvergleich wurde eine konservierte zweiseitige Kernlokalisationssequenz im Terminalen Protein der HBV Polymerase identifiziert, die eine Proteinkinase CKII Erkennungsstelle enthält. Inhibition der CKII Aktivität in HBV infizierten primären Hepatozyten sowie die Zerstörung der CKII Erkennungsstelle im Terminalen Protein inhibieren die HBV Replikation. Die Funktionalität der Kernlokalisationssequenz wurde durch Fusion an GFP bestätigt und war Abhängig von CKII Aktivität in der Zelle. Dies wurde in vitro durch Bindung des Adapterproteins Karyopherin-alpha an CKII-phosphoryliertes Terminales Protein bestätigt. Die HBV Polymerase enthält eine konservierte zweiseitige Kernlokalisationssequenz deren Funktionalität durch CKII Phosphorilierung vermittelt wird. / Hepatitis B virus (HBV) infection causes acute and chronic liver inflammation. Especially the early phase of the HBV life cycle is not clearly understood. For example the receptor complex that mediates viral entry is not known. Novel infection models to study the HBV lifecycle are described that demand for a large amount of cell culture generated infectious HBV particles. One aim was to enhance HBV production of the cell line HepG2.2.15 by cultivation on microcarrier substrate. Analysis of protein and viral particle secretion, infectivity, and cellular MAP kinase signaling revealed an up to 18x increased HBV production and a decreased subviral particle secretion by HepG2.2.15 when cultivated on microcarrier. The observed effect was due to an enhanced phospho-activation of MAP kinase ERK-2 that is tightly associated with HBV replication. Another poorly understood part of the HBV lifecycle is the mechanism that delivers the HBV genome into the nucleus. Traces of HBV polymerase can be found in HBV infected cells. The second objective was to identify motifs on the HBV polymerase that determine its subcellular localization. By sequence alignment a conserved bipartite nuclear localization signal was found in the terminal protein of the HBV polymerase encompassing a protein kinase CKII recognition site. Inhibition of CKII kinase in infected primary hepatocytes and destruction of the identified CKII recognition site in the viral polymerase impaired virus production. The functionality of the putative nuclear localization signal was confirmed by fusion to GFP. Moreover, its functionality was depended on CKII activity that was verified by in vitro binding experiments of terminal protein to the import adaptor karyopherin-alpha. This data identified a nuclear localization signal in the HBV polymerase, which functionality is mediated by CKII phosphorylation.

We consider the Cauchy problem for the focusing energy critical NLS with inverse square potential. The energy of the solution, which consists of the kinetic energy and potential energy, is conserved for all time. Due to the focusing nature, solution with arbitrary energy may exhibit various behaviors: it could exist globally and scatter like a free evolution, persist like a solitary wave, blow up at finite time, or even have mixed behaviors. Our goal in this thesis is to fully characterize the solution when the energy is below or at the level of the energy of the ground state solution $W_a$. Our main result contains two parts. First, we prove that when the energy and kinetic energy of the initial data are less than those of the ground state solution, the solution exists globally and scatters. Second, we show a rigidity result at the level of ground state solution. We prove that among all solutions with the same energy as the ground state solution, there are only two (up to symmetries) solutions $W_a^+, W_a^-$ that are exponential close to $W_a$ and serve as the threshold of scattering and blow-up. All solutions with the same energy will blow up both forward and backward in time if they go beyond the upper threshold $W_a^+$; all solutions with the same energy will scatter both forward and backward in time if they fall below the lower threshold $W_a^-$. In the case of NLS with no potential, this type of results was first obtained by Kenig-Merle \cite{R: Kenig focusing} and Duyckaerts-Merle \cite{R: D Merle}. However, as the potential has the same scaling as $\Delta$, one can not expect to extend their results in a simple perturbative way. We develop crucial spectral estimates for the operator $-\Delta+a/|x|^2$, we also rely heavily on the recent understanding of the operator $-\Delta+a/|x|^2$ in \cite{R: Harmonic inverse KMVZZ}.

No description available.

L’étude des ondes scélérates est un sujet en plein essor principalement en océanographie mais également dans d’autres domaines. Dans cette thèse, je construis par transformation de Darboux une famille multi-paramétrique de solutions quasi-rationnelles lisses de l’équation de Schödinger non linéaire qui présentent un comportement d’ondes scélérates. Pour un choix générique de paramètres les solutions de deuxième ordre donnent un modèle de "trois sœurs" (une succession de trois vagues plus hautes que prévues) alors que pour un choix particulier de paramètres on obtient les solutions présentées par Akhmediev et al. dans une série d’articles de 2009. Ces solutions me permettent ensuite de construire des solutions rationnelles de l’équation KP-I qui décrit le mouvement des vagues dans une eau peu profonde. / The study of rogue waves is a booming topic mainly in oceanography but also in other fields. In this thesis I construct via Darboux transform a multi-parametric family of smooth quasi-rational solutions of the nonlinear Schödinger equation that present a behavior of rogue waves. For a general choice of parameters the second-order solutions give a model of "three sisters" (three higher than expected waves in a row) while for a particular choice of parameters we obtain the solutions given by Akhmediev et al. in a serie of articles in 2009. Then these solutions allow me to construct rational solutions of the KP-I equation that describe waves in shallow water.

京都大学 / 新制・課程博士 / 博士(工学) / 甲第23482号 / 工博第4894号 / 新制||工||1765(附属図書館) / 京都大学大学院工学研究科社会基盤工学専攻 / (主査)教授 森 信人, 准教授 原田 英治, 准教授 志村 智也 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DFAM

A method of sensor location selection is introduced for distributed parameter systems. In this method, the sensitivities of spatial outputs to model parameters are computed by a model and transformed via continuous wavelet transforms into the time-scale domain to characterize the shape attributes of output sensitivities and accentuate their differences. Regions are then sought in the time-scale plane wherein the wavelet coefficient of an output-sensitivity surpasses all the others’ as indication of the output sensitivity’s uniqueness. This method yields a comprehensive account of identifiability each output provides for the model parameters as the basis of output selection. This output selection strategy is evaluated for a numerical case of pollutant dispersion by advection and discussion in a two-dimensional area.

The focus of this dissertation is the existence, stability, and resulting dynamical evolution of localized stationary solutions to Nonlinear Schr¨odinger (NLS) equations with periodic confining potentials in 2(+1) dimensions. I will make predictions about these properties based on a discrete lattice model of coupled ordinary differential equations with the appropriate symmetry. The latter has been justified by Wannier function expansions in a so-called tight-binding approximation in the appropriate parametric regime. Numerical results for the full 2(+1)-D continuum model will be qualitatively compared with discrete model predictions as well as with nonlinear optics experiments in optically induced photonic lattices in photorefractive crystals. The predictions are also relevant for BECs (Bose-Einstein Condensates) in optical lattices.

<p>A model with nonlinear Schrödinger (NLS) equation used for describing pulse propagations in photopolymers is considered. We focus on a case in which change of refractive index is proportional to the square of amplitude of the electric field and consider 2-dimensional spatial domain. After formal derivation of the NLS approximation from the wave-Maxwell equation, we establish well-posedness and perform rigorous justification analysis to show smallness of error terms for appropriately small time intervals. We conclude by numerical simulation to illustrate the results in one-dimensional case.</p> / Master of Science (MSc)

In healthy cells, huntingtin is primarily found in the cytoplasm; however, upon cellular stress, huntingtin is phosphorylated (phospho-huntingtin) at serines 13 and 16 of the amino-terminal N17 domain and shuttled into the nucleus. Such dynamism in nucleocytoplasmic translocation and post-translational modification suggests an important role for huntingtin in Huntington’s disease (HD) pathogenesis as these phenotypes propose possible mechanisms for disease progression. Huntingtin nuclear import is also facilitated by its proline-tyrosine nuclear localization signal (PY-NLS), which harbours a highly conserved intervening sequence specific to the huntingtin gene. This encouraged a proteome investigation to identify potential protein partners of the PY- NLS. Results of this study revealed that high mobility group box 1 (HMGB1), a cofactor of base excision repair, uniquely bound to the wild-type PY-NLS, but not the PY-NLS KK177/178AA mutant. Immunofluorescence microscopy in human telomerase reverse transcriptase (hTERT) immortalized fibroblast cells using HMGB1- and phospho- huntingtin-specific antibodies revealed a promising association between the two, as changes in nuclear levels of HMGB1 positively correlated with nuclear levels of phospho- huntingtin. This relationship was further confirmed by co-immunoprecipitation of HMGB1 by the PY-NLS and N17 domain. Also, when exogenous oxidative stress was introduced, increased interaction between HMGB1 and huntingtin was observed. This suggests that HMGB1 facilitates the nuclear import of huntingtin in a ROS-dependent manner, and thus, presents a novel avenue to a potential therapeutic target in HD pathogenesis. / Thesis / Master of Science (MSc)