Thesis (D.M.A.)--University of North Texas, 2005. / System requirements: Adobe Acrobat Reader. Accompanied by 4 recitals, recorded Apr. 15, 2002, Mar. 10, 2003, Feb. 16, 2004, and Apr. 25, 2005. Includes bibliographical references (p. 89-90).

Made available in DSpace on 2014-06-11T19:28:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-06-22Bitstream added on 2014-06-13T19:16:44Z : No. of bitstreams: 1 moura_mf_me_botfca.pdf: 702744 bytes, checksum: d94bcbccf8a2ddefe768c5c679f20208 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O pimentão (Capsicum annuum L.) está entre as dez hortaliças mais consumidas no Brasil. Dois potyvirus são verificados nesta cultura, o Potato virus Y (PVY) e o Pepper yellow mosaic virus (PepYMV). Foi avaliada entre outubro de 2007 a novembro de 2008, a ocorrência de potyvirus infectando Capsicum spp. nos municípíos de Pirajú, Pirajuí, Paranapanema, Santa Cruz do Rio Pardo, Sorocaba, São Miguel Arcanjo, Itapetininga, Reginópolis, Lins, Iacanga e Mogi-Mirim, do Estado de São Paulo. Das 408 amostras coletadas, 105 foram positivas para a presença de potyvirus utilizando-se antissoro antipotyvirus (Agdia). Em algumas amostras foi detectada a presença de infecção mista com o Cucumber mosaic virus (CMV) e begomovírus. A inoculação de cinqüenta e um isolados na série diferencial de Capsicum spp contendo os genes pvr21, pvr22 e Pvr4, dos quais dez foram provenientes da Empresa Sakata Seed Sudamérica, possibilitou a classificação de dois isolados em patótipo 0, três em patótipo 1, seis em patótipo 1.2, onze em patótipo 1.2.3 e treze em patótipo 1-3 de PVY. Dezesseis isolados não puderam ser classificados em patótipos. Não foi verificada correlação entre local de coleta e ocorrência de um patótipo específico, evidenciando grande variabilidade biológica dos isolados de potyvirus no campo. Nenhum dos isolados coletados ocasionou sintomas evidentes em plantas de pimentão Rubia R e Magali R, indicando que a resistência conferida por estes híbridos ainda é efetiva contra os isolados de potyvirus predominantes no campo. Um par de primers PepNib (5’ GWTSGYYGMMTTGGATGATG 3’) e PepUTR (5’ AGTAGTACAGGAAAAGCC 3’) foi 2 obtido para amplificação completa da região codificadora da proteína capsidial de PVY e PepYMV. Analisando-se esta região do genoma viral, pôde-se constatar predominância da espécie PepYMV. O PVY foi encontrado somente em coletas... / The sweet pepper (Capsicum annuum L.) is one of the ten most consumed vegetables in the country. In Brazil, two potyviruses are verified in this culture, the Potato virus Y (PVY) and the Pepper yellow mosaic virus (PepYMV). Between October 2007 and November 2008, the occurrence of potyvirus infecting Capsicum spp. was evaluated on plants collected from Piraju, Pirajuí, Paranapanema, Santa Cruz do Rio Pardo, Sorocaba, São Miguel Arcanjo, Itapetininga, Reginópolis, Lins, Iacanga and Mogi-Mirim, in Sao Paulo State. Among the 408 collected samples, 105 were positive for the presence of potyvirus using antipotyvirus antiserum (Agdia). The presence of mixed infection with the Cucumber mosaic virus (CMV) and begomovirus was also verified. The inoculation of fifty one isolates on the series of Capsicum spp. containing the genes pvr21, pvr22 and Pvr4, ten of them isolates from the Sakata Seed Sudamerica Company, made it possible the classification of these isolates in different pathotypes. Two isolates were classified as pathotype 0, three in pathotype 1, six in 4 pathotype 1.2, eleven in pathotype 1.2.3 and thirteen in pathotype 1.3 of PVY. Sixteen isolates were not able to be classified in pathotypes of PVY. No correlation could be made between the origin of the isolate and the presence of an specific pathotype, indicanting a greet biological variability between the potyvirus isolates. None of the isolates collected in the field caused symptoms in Rubia R and Magali R, indicating that the resistance provided by these hybrids is still effective against the predominant isolates of potyvirus. A pair of primer PepNib (5’ GWTSGYYGMMTTGGATGATG 3’) and PepUTR (5’ AGTAGTACAGGAAAAGCC 3’) were obtained for the complete amplification of the capsid protein region of PVY and PepYMV isolates. PepYMV was the prevalent species of potyvirus found infecting sweet peppers. The PVY was found only... (Complete abstract click electronic access below)

Master of Science / Department of Horticulture and Natural Resources / Cary L. Rivard / Tomatoes and peppers are the most popular and profitable high tunnel crops. However, year-round intensive cultivation and extensive monocropping can lead to a loss of soil quality and the buildup of soilborne pathogens. Many growers are considering grafting to help address the drawbacks of covered agriculture and improve yields. Although many trials have been conducted that examine the ability of rootstock to increase yield or reduce disease, the effect of scion cultivar has yet to be tested, and few studies have attempted to quantitatively assess scion compatibility. In 2016 and 2017, we evaluated ten hybrid, determinate, red slicing tomato scion cultivars for compatibility with ‘Maxifort’ rootstock in a three-season high tunnel in Olathe, KS. While all ten varieties were compatible with ‘Maxifort’, only ‘BHN 589’, ‘Red Deuce’, ‘Skyway’, and ‘Tasti Lee’ were “highly compatible” and showed significant improvements in marketable yield when grafted. Additionally, when ranked by yield, differences between grafted and nongrafted populations suggest that relative compatibility may be inconsistent between varieties. However, a significant inverse relationship between the yield of the nongrafted plants and the percent yield benefit from grafting indicates that the effect of a rootstock like ‘Maxifort’ may not be synergistic, with higher performing nongrafted scion varieties benefitting less from grafting than lower performing varieties. ‘Red Deuce’ and ‘BHN 589’ are productive, and highly compatible grafted varieties with potential for commercial high tunnel production. ‘Primo Red’ benefitted the least from grafting but was the highest performing nongrafted variety (outperforming four of the grafted combinations). Compared to tomatoes, published reports on grafted peppers have been limited and it is unclear whether they provide any advantage in the absence of soilborne disease or environmental stress. Additionally, the use of rootstocks from other solanaceous species outside the Capsicum genus for pepper grafting has not been well explored, though the pool of available rootstock options for peppers would be substantially increased if such graft unions proved to be compatible. The goals of a second project were to identify the utility of grafted pepper (C. annum) plants for commercial high-tunnel production and to explore the potential for graft compatibility between the Capsicum and Solanum genera. We grafted ‘Karisma’ bell peppers onto two Solanum cultivars (‘Maxifort’ and ‘Sweetie’) and three pepper rootstocks (‘Scarface’, ‘Meeting’, and ‘Yaocali’). Five trials were conducted in 2016-2017 and utilized a randomized complete block design in all locations. Plants grafted onto Solanum rootstocks displayed symptoms of delayed incompatibility, including significant (78%-89%) reductions in yield (by weight), 59%-93% less plant growth, and 58% less marketability, as well as malformations at the graft union and higher in-field mortality rates. These symptoms were likely due to differences in mature stem anatomy. Plants grafted to ‘Scarface’ produced 32% greater marketable yield, 15%-18% larger fruit, and 9-12% higher marketability than nongrafted ‘Karisma’. The results for ‘Yaocali’ were similar to ‘Scarface’, though less conclusive. While ‘Yaocali’ and ‘Scarface’ rootstocks may be useful for improving yield in low-stress environments, the use of ‘Meeting’ may be more beneficial for combatting disease.

En el presente trabajo de investigación se evalúa la viabilidad para la implementación de una empresa productora y comercializadora de crema de ají elaborada a base de insumos como el ají pipi de mono y la castaña. En la etapa de análisis externo se reveló que el 89% de peruanos acompañan sus comidas con alguna crema picante y que el consumo per cápita del país es solo del 4.75 kg lo cual demuestra nuevas oportunidades de crecimiento. En la etapa de investigación y validación se determinó la falta de variedades de cremas de ají y la aceptación del producto, esto como resultado al estudio de mercado realizado a las amas de casa entre las edades de 18 a 55 años de los NSE A y B dentro de Lima metropolitana. En la etapa del plan de marketing se determina el cálculo del target, para luego definir el market share, asimismo, se determinó el crecimiento de la demanda del 10% del market share para el primer año el cual contará con un crecimiento del 7% anual de acuerdo al crecimiento de mercado por los próximos años. Finalmente se desarrolló el plan financiero para los próximos 5 años, en el cual se determinó la inversión y financiamiento requerido. Igualmente se determinó los ingresos y egresos, lo que permitió la elaboración de los estados financieros y el análisis de sensibilidad, así mismo mediante los indicadores de rentabilidad se obtuvo un VPN de S/ 38,049, y una TIR de 29%. / In the present research work the viability is evaluated for the implementation of a company that produces and sells chilli cream made from raw materials such as “pipi de mono” chili peppers and chestnuts. In the external analysis stage, it was revealed that 89% of Peruvians accompany their meals with some spicy cream and that the per capita consumption of the country is only 4.75 kg, which demonstrates new opportunities for growth. In the research and validation stage, the lack of varieties of chili creams and the acceptance of the product was determined, as a result of the market study carried out on housewives between the ages of 18 and 55 years of socioeconomic level A and B within metropolitan Lima. In the stage of the marketing plan the target calculation is determined, to then define the market share, in the same way, the growth of demand of 10% of the market share for the first year was determined, which will have an annual growth of 7% according to the market growth for the coming years. Finally, the financial plan for the next 5 years was developed, in which the required investment and financing was determined. Likewise, income and expenses were determined, which allowed for the preparation of the financial statements and the sensitivity analysis. As well, through the profitability indicators, a NPV (Net Present Value) of S/ 38,049 was obtained and an IRR (Internal Rate of Return) of 29%. / Trabajo de investigación

No description available.

The effective dispersal ability of the egg parasitoid Trichogramma ostriniae Pang and Chen was assessed in potato fields on the Eastern Shore of Virginia in spring 2005 and 2006. Approximately 0.5 million T. ostriniae were released from a central release point in separate potato fields. Dispersal was monitored using yellow sticky card traps and European corn borer, Ostrinia nubilalis Hübner, egg mass sentinels. Adult T. ostriniae dispersed quickly throughout the 0.4 ha (1 acre) sampling area. Parasitism and sticky card captures were highest close to the release point and decreased with increasing distance. Sticky card data were a good fit to the diffusion model used (r² > 0.90) for all but two sampling dates. In 2005 parasitization peaked at 4 days post release with close to 40% of sentinels parasitized at 30m from the release point. The mean distance encompassing 98% (x98) of T. ostriniae for both fields in 2005 was 27.5 (± 2.4) meters. For fields 1 and 2 in 2005, x98 for parasitism was 21 and 26 meters, respectively. In 2006 sticky card data fit the dispersal model moderately well (r² > 0.77) except for two sampling dates and dispersal was generally lower. The mean x98 value for sticky card data was 12.9 (± 0.9) meters. For parasitism, the x98 distances for field 1 and 2 were estimated at 8 and 10 meters, respectively. Correlation analysis showed no significant difference in the distributions between sticky card captures and sentinel egg mass parasitism. In 2006, T. ostriniae were released in small pepper plots in Pennsylvania, Maryland and two locations in Virginia to evaluate the number of wasps needed per plant for effective control of European corn borer. Treatments included 0, 5, 20 and 50 wasps per plant. In each plot, parasitism was measured using 30 sentinel egg masses collected on 3 and 6 days post release. Parasitism was relatively low in Pennsylvania and Virginia and no significant effect from release density was observed. High rates of parasitization in the untreated control plot were observed in Maryland as well as one of the Virginia locations. Overall results show results show ambiguity in the data and high levels of natural parasitism occurring on Ephestia eggs sentinels. In 2005 and 2006, several insecticides were evaluated for controlling O. nubilalis and impacting arthropod natural enemies in bell pepper. In addition, we compared the effectiveness of an integrated pest management program based around inundative releases of T. ostriniae to a conventional insecticide-based program for O. nubilalis control in multiple locations in the Mid- Atlantic US. To evaluate the insecticides, small plots of bell pepper were established at four locations in Virginia, Maryland, Delaware, and Pennsylvania. Insecticides were applied weekly from first fruit until final harvest (5 to 7 applications). Results indicated that the biorational insecticides, spinosad, indoxacarb, and methoxyfenozide provided comparable control of O. nubilalis as the broadspectrum conventional insecticides, acephate, and lambda-cyhalothrin. At most locations, multiple sprays of lambda-cyhalothrin resulted in flares (outbreaks) of green peach aphids most likely from destruction of arthropod natural enemies. Indoxacarb also caused a similar aphid flare at one of the locations. For the IPM demonstration experiment, pepper plots were established at 5 locations in the Mid-Atlantic U.S. in 2005 and 2006. Treatments included: "conventional", which involved weekly applications of acephate or lambda-cyhalothrin from first fruit until final harvest; 2) "IPM", which included three or four inundative releases of T. ostriniae and a judicial application of methoxyfenozide only if lepidopteran pests exceeded action thresholds; and 3) an untreated control. No significant treatment effect was found in either year on cumulative number of marketable fruit or percentage of fruit damaged by lepidopteran pests. A significant treatment effect was found on peak numbers of green peach aphids, with the conventional insecticide approach causing aphid flares and the untreated control or IPM approach not having aphid pest problems. Inundative releases of T. ostriniae may be a more environmentally-sound approach to managing O. nubilalis in peppers, although a comparison with conventional insecticides under greater lepidopteran pest pressure is still needed. / Master of Science in Life Sciences

Salmonella embedded biofilms were formed on whole black peppercorns and treated with ethanol vapor under atmospheric pressure conditions and under vacuum assisted pressure conditions. The effect of ethanol vapor, heat and vacuum pressure on the survival of multiple Salmonella enterica serovars within a biofilm contained on low moisture food products (Aw 0.30-0.40) was observed in this study. Samples were exposed to zero, one, five and ten minute ethanol vapor treatments at atmospheric boiling temperature (Atmospheric Pressure Boiling Method, AB), and at reduced temperature and pressure conditions, -20 inHg (Vacuum-Assisted Boiling Method, VB). The AB treatments showed 4.0 log CFU/g reductions on nonselective media that included native microbiota, and 6.0 log CFU/g reductions on Salmonella selective media. The VB treatments showed 2.69 log reductions on Tryptic Soy Agar and 4.55 log reductions on Xylose Lysine Tergitol-4 agar. Ethanol vapor treatments should be further investigated as an alternative to ethylene oxide or ionizing radiation processes to treat dry spices to control Salmonella. / Master of Science in Life Sciences

No description available.

No description available.

O oídio do pimentão e pimenteiras, causado pelo fungo Leveillula taurica, tem sido considerado atualmente a doença mais séria do cultivo protegido, causando perdas de até 75%. O uso de fungicidas é ineficaz sendo a melhor maneira de controle através da resistência genética. As fontes de resistência ao oídio encontradas em Capsicum annuum L., não tem sido satisfatórias por apresentarem resistência parcial e as melhores fontes ocorrem nas espécies C. baccatum e C. chinense. Existem poucos estudos sobre a herança da resistência nessas espécies. O objetivo deste trabalho foi de triar os acessos da coleção de Capsicum da ESALQ/USP, identificando possíveis fontes de resistência nas espécies C. annuum, C. baccatum e C. chinense e obter informações sobre a herança da reação de pimentão e pimentas ao oídio. Para isso 156 acessos de Capsicum foram triados e posteriormente cruzamentos entre progenitores resistentes versus suscetíveis e resistentes versus resistentes foram obtidos. As gerações F2 e alguns retrocruzamentos foram obtidos. As avaliações das reações ao oídio foram feitas na fase de frutificação, através de uma escala de notas de acordo com a severidade da doença de 1 (resistente) a 5 (altamente suscetível). Foram estimados: o número de locos segregantes, os coeficientes de herdabilidade, os progressos esperados na seleção e o tipo de ação gênica envolvida. Foram avaliadas também as reações dos híbridos entre progenitores resistentes. Dentre os materiais triados em C. annuum apenas 5% foram considerados resistentes, em C. baccatum 84% e em C. chinense 85%. A herança da resistência aooídio em Capsicum mostrou ser poligênica. As herdabilidades e ganhos de seleção estimados foram altos, indicando boas perspectivas de progresso seletivo. Os progenitores resistentes em Capsicum annuum possuem diferentes mecanismos genéticos e expressões de resistência. Enquanto em C. baccatum e C. chinense os mecanismos de resistência mostraram ser os mesmos, havendo alelismo entre as fontes. A fonte de resistência do pimentão HV-12 foi a melhor para o uso no melhoramento. / Powdery mildew caused by the fungus Leveillula taurica, is considered the most serious greenhouse sweet pepper disease, causing losses up to 75%. Its control by fungicide is ineffective, and the best way to control would be by genetic resistance. Powdery mildew resistance sources identified in Capsicum annuum L. have been unsatisfactory because they have partial resistance. The best resistant sources seem to be found in the species C. baccatum and C. chinense. However, the resistance inheritance has not been studied in these hot pepper species, except for C. annuum, with few published pappers. The present study aimed to screen the Capsicum ESALQ/USP collection accesses, to identify potential resistance sources in C. annuum, C. baccatum and C. chinense and elucidate the reaction inheritance to powdery mildew in sweet pepper and hot pepper. A total 156 Capsicum access were tested. Crosses among resistant and susceptible progenitors and among resistant ones were carried out. F2 and backcrosses generations were obtained. Reactions evaluations to powdery mildew were carried out during the fruit production using rating system based on score disease severity using scale varying from 1 (resistant) to 5 (highly susceptible). Estimation of segregating loci number, heritability and expected selection gains was made, to investigate the gene action and to evaluate the reaction to powdery mildew from resistant versus resistant single hybrids. Only 5% of the C. annuum accesses evaluated were considered to be resistant, in contrast with 84% and 85% obtained for C. baccatum and C. chinense, respectively. Resistance inheritance to powdery mildew in Capsicum spp. was found to be polygenic. In view of the high heritability and selection gains estimates, good selection progresses are expected. The genetic systems controlling resistance in the Capsicum annuum progenitors have different genetic mechanisms and expressions of resistance, while in C. baccatum and C. chinense showed to be the same in each ones with allelism between the sources. The sweet pepper HV-12 was the best source to use in breeding.