Pinus radiata is by far the dominant species grown in New Zealand plantations as a renewable source of wood. Several wood quality issues have been identified in the material produced, including the high incidence of compression wood, which is undesirable for end users. At present our understanding of the complex array of developmental processes involved in wood formation (which has a direct bearing on wood quality) is limited. Hence, the forest industry is interested in attaining a better understanding of the processes involved. Towards this goal, and for reasons of biological curiosity, the experiments described in this thesis were carried out to investigate several aspects of xylem cell development. In an in arbor study, changes in the orientation of cortical microtubules and cellulose microfibrils were observed in developing tracheids. Results obtained provide evidence that cortical microtubules act to guide cellulose synthase complexes during secondary wall formation in tracheids. The mechanisms involved in controlling cell wall deposition in wood cells are poorly understood, and are difficult to study, especially in arbor. A major part of this thesis involved the development of an in vitro method for culturing radiata pine wood in which hormone levels, nutrients, sugars and other factors, could be controlled without confounding influences from other parts of the tree. The method developed was used in subsequent parts of this thesis to study compression wood development, and the influence of the hormone gibberellin on cellulose microfibril organisation in the cell wall. Results from the in vitro compression wood experiments suggested that: 1. when a tree is growing at a lean, the developing cell wall was able to perceive compressive forces generated by the weight of the rest of the tree, rather than perceive the lean per se. 2. ethylene, rather than auxin, was involved in the induction of compression wood. Culture of stem explants with gibberellin resulted in wider cells, with steeper cortical microtubules, and correspondingly steeper cellulose microfibrils in the S2 layer of developing wood cells. This observation provides further evidence that the orientation of microtubules guides the orientation of cellulose microfibrils. Overall, the work described in this thesis furthers our knowledge in the field of xylem cell development. The stem culture protocol developed will undoubtedly provide a valuable tool for future studies to be carried out.

Long-lived trees and excellent preservation of remnant wood allow examination of late-Holocene climate variability and its relation to woodland tree demography and populations of prehistoric agriculturalists in northeastern Utah using dendrochronological methods. Tree-ring chronologies are developed from Douglas-fir (Psuedotsuga menziesii) and Colorado pinyon (Pinus edulis) on the Tavaputs Plateau covering the last 2,300 years. The climate reconstructions fill an important temporal and spatial gap in our understanding of moisture related climate variability in the region. We investigate the relationships between climate and woodland demography by constructing a 1,500-year record of pinyon establishment and death. Twentieth-century expansion and infill of pinyon/juniper woodlands and more recent widespread die-offs in the early 21st century heighten the importance of understanding these relationships. The climate reconstruction is analyzed in light of the archaeological record of Fremont agriculturalists between approximately AD 550 and 1300, and provides the first glimpse of climate variability throughout the Fremont era in this region.Results of the hydroclimate reconstructions show that multidecadal droughts unlike any observed in the instrumental record occur regularly over the last 2,000 years. Droughts in the mid 12th century and late 13th century are synchronous those found in numerous other records across the southwestern United States. A drought in the early 6th century is especially severe. Analysis of pinyon demography indicates rates of tree establishment, release, and death are highly variable over the last 1,500 years. Broad peaks in tree establishment occur in the 7th and 8th centuries, the 12th and 13th centuries, and again in the 18th, 19th and 20th centuries. Higher tree death rates are related to dry periods, but tree establishment is only weakly associated with wetter periods. Instead, cohorts of suppressed young trees established over decades tend to synchronously experience rapid growth rate increases during wet periods following droughts. Stands appear more susceptible to population turnover as semi-dominant cohorts of trees age and decline. Two critical periods in Fremont archaeology in the region, coincide with significant changes in moisture conditions. These changes follow longer periods of stability suggesting that changes in the predictability of climate conditions may have impacted Fremont agriculturalists in the region.

We examined tree rings from cross-sections of shortleaf pine (Pinus echinata Mill.) timbers extracted from a house in Forsyth County, Georgia, that was reportedly built in the mid-19th Century during the Antebellum Period (pre-1860). Our goals were to (1) determine the probable construction year for the house to help assess its possible historical significance, and (2) create a new long-term reference chronology for the northern Georgia area where such chronologies are lacking. Sections of shortleaf pine were removed from the structure during a renovation project in 2001. Sixteen sections were used to build a floating tree-ring chronology 217 years in length from series that crossdated conclusively with other series both graphically via skeleton plots and statistically via COFECHA. We then statistically evaluated the probable absolute temporal placement of this chronology using several regional tree-ring chronologies from the southeastern U.S. A statistically significant (p , 0.0001) correlation between our chronology and a shortleaf pine chronology from Clemson, South Carolina, anchors our chronology between 1652–1868. Two missing rings are probable in the early portion of our chronology, but we currently do not have a sufficient number of samples to conclusively identify their exact placement. No cluster of outermost rings was found to support the reported construction date of 1851, although the outermost rings on 13 of 16 samples dated before 1851. This new chronology could aid further dating of wood from archaeological sites and historical structures, and establish an initial data set that could eventually provide important new insights about the climate of northern Georgia during the 17th–19th Centuries.

Two Chinese pine (Pinus tabulaeformis) tree-ring width chronologies up to 375 years long were used to reconstruct rainfall from February to early July for the Wu Dangzhao region and from February to mid-July for the La Madong region, Inner Mongolia, China. The predictor variables account for 44.3% and 42.7% of the variance in precipitation, respectively. Both historical records and two other tree-ring based precipitation reconstructions from the environmentally sensitive zone (the northern Helan Mountain range and Baiyinaobao) confirm our results. After applying a 10-year moving average, the trends of four tree-ring based precipitation reconstructions vary synchronously. Periods with below-normal precipitation occurred during the 1720s–1730s, 1740s–1750s, 1790s, early 1810s, late 1830s–1860s, 1880s–1910s, late 1920s–1930s and after the late 1960s–early 1970s. Periods with above-normal precipitation occurred in the 1760s to early 1770s, 1820s to early 1830s, 1870s–1880s, early 1920s, 1940s to early 1960s, and 1990s. The late 1920s period was the most severe drought over a broad area in north China in the last 375 years. In contrast, the wettest period was in the late 1990s.

The cutting dates of 10 wood timbers (girders and corner rafters) of Sinmu-mun, the north gate of Kyungbok Palace in Seoul, were determined by the dendrochronological method. Tree-ring chronologies of unknown dates derived from the timbers were crossdated using the graphic comparison method against the dated master chronologies derived from living trees. The living trees for the masters used for this study were Pinus densiflora Sieb. et Zucc. (Japanese red pine), a major timber species for Korean traditional buildings. By comparing the Sinmu-mun samples with the masters from the western Sorak Mountains in central-eastern Korea, the Sinmu-mun samples yielded the cutting dates A.D. 1868, 1869, and 1870/1871. Surprisingly, these dates are 3 to 6 year later than the known date (A.D. 1865) of the Sinmu-mun reconstruction, which was recorded in a historical document ‘Ilsungrok’, the King’s official diary. Since the time that the Sinmu-mun construction date had been questioned, another record was found in the 1872 April issue of Ilsungrok, indicating the rebuilding of Sinmu-mun in the 1870s. Both pieces of evidence, from tree-ring dates and historic records, prove that the rebuilding of Sinmu-mun started after the Fall of 1870, but not later than April 1872. The results prove that tree-ring dating is a precise dating method and it can be applied to archaeological studies on Korean structures.

Le bois mort a été retenu comme l’un des indicateurs de biodiversité pour la gestion durable des forêts. L’objectif de la thèse est de tester la pertinence de cet indicateur dans le contexte des forêts de plantation grâce à l'acquisition de données expérimentales. L’approche est focalisée sur les Coléoptères qui représentent 20% des espèces saproxyliques. La richesse spécifique et la composition des assemblages sont influencées par des variables à 3 échelles: la pièce de bois mort (diamètre, stade de décomposition et type), le peuplement (volume de bois mort, densité, couvert) et le paysage (% de couvert en pin maritime, feuillus et coupe rase). La diversité des pièces de bois mort de diamètre supérieur à 15cm constitue un bon indicateur de la richesse locale en Coléoptères saproxyliques. Un modèle de dynamique du bois mort a été développé, permettant d’évaluer les incidences potentielles de nouvelles pratiques sylvicoles sur la biodiversité des Coléoptères saproxyliques. / Dead wood has been selected as one of the biodiversity indicators for the assessment of sustainable forest management. We investigated the relevance of this indicator in forest plantations with experimental data. Our approach was focus on beetles which represent 20% of saproxylic species. Effects of environmental variables on species richness and composition of assemblages occurred at three spatial scales : the piece of wood (diameter, decomposition stage and type), the stand (volume of dead wood, density of stems, canopy cover) and the landscape (% of maritime pine stands,% of deciduous stands and % of clear-cut areas). Diversity of dead wood above 15 cm in diameter appeared to be a good indicator of the local species richness of saproxylic beetles. A model of dead wood dynamic has been proposed so as to assess impacts of new forestry practices on saproxylic beetles diversity.

The remnants of the Caledonian Pine Forest represent the north western boundary of the Eurasian Pinus sylvestris (L.) distribution. Remnant populations occupy a diverse range of environments within Scotland, subject to a steep rainfall gradient, and previous investigations have found evidence of local adaptation. Additionally, studies of biochemical and molecular markers have indicated that Scotland’s native pinewoods originated from more than one glacial refugium. Whole-genome-shotgun (WGS) sequencing was employed for the discovery of mitochondrial (mt) variants that may provide further insight into the origins of P. sylvestris populations both in Scotland and mainland Europe. DNA extractions were performed on megagametophyte tissue from Scottish, Finnish, and Spanish populations. Three members of the closely related P. mugo species complex were also sequenced. Using similarity-based approach, 160kbp of putative mitochondrial sequence was recovered by comparison of de novo assembled contigs with the mtgenome of the gymnosperm Cycas taitungensis. In total, 16 novel variants were identified among samples, which may be used in future phylogeographic studies. A study of needle characters was performed for eight native populations of P. sylvestris in an outdoor provenance/progeny trial of 192 saplings. A negative correlation was detected between longitude and the number of stomatal rows present on needle surfaces. It was posited that this may be an adaptive response to lower water availability in eastern pinewoods, possibly in conjunction with increasing altitude. The west coast of Scotland is one of the wettest regions in Europe: western pinewoods may receive in excess of 3,000mm of rainfall in a year, compared with an average of 800mm eastern sites. To determine whether native pinewoods are differentially adapted to waterlogging, a glasshouse based provenance/progeny trial of 432 saplings from nine native populations was undertaken, in which 50% were subject to a long-term waterlogging treatment, and the remainder used as a control. Two studies were then conducted. In the first, responses to the treatment were assessed in terms of phenological and growth traits. Bud flush was delayed in response to waterlogging, and growth was impeded relative to the control. Although population differences were observed, treatment × population interactions were not detected. In the second study physiological traits known to be sensitive to plant stress and water balance were measured at intervals throughout the experiment. Prior to the commencement of the treatment needle δ13C was found to exhibit interpopulation differentiation, and was positively correlated with longitude. This seems likely to represent differential selection for water use efficiency between eastern and western pinewoods. Photochemical efficiency and stomatal conductance were found to be reduced by waterlogging, and needle δ13C was increased. After generalising populations into ‘high’ and ‘low’ rainfall groups (monthly averages of 214.9mm and 72.8mm, respectively), high rainfall populations were observed to maintain consistently higher photochemical efficiency under waterlogging the low rainfall populations. In addition, the low rainfall group exhibited greater variability in response to flooding (in terms of phenotypic and additive genetic variance) which may be indicative of a lack of past selection pressure.

Master of Science - Science / The replacement of indigenous grasslands by commercial forestry plantations on the mountainous eastern escarpment of South Africa, has inevitably led to alteration of nutrient cycling. The overall objective of this study was to determine the nutrient status (soil, litter and foliar nutrient concentrations) of Pinus patula and Eucalyptus grandis plantations and adjacent grasslands. The 2, 13 and 30 year old P. patula plantations and adjacent Driekop grassland were located relatively high up on the escarpment (altitudes ranging between 950–1610 m) and were underlain by dolomite while the 2, 8 and 13 year old E. grandis plantations and adjacent Brooklands grassland were located lower down (altitudes ranging between 900–1100 m) and were underlain by granite. It was assumed that the nutrient status of the soil under the plantations and the adjacent grasslands were similar prior to afforestation. Samples were collected once in winter (August 2002) and once in summer (January 2003). Soil samples were collected to a depth of 20 cm and litter material, to a maximum depth of 20cm. Lower canopy foliar samples were collected within the lowermost metre of the canopies and upper canopy foliar samples were collected within the uppermost metre of the canopies. The data were analysed in the laboratory for various elements and soil physical parameters. The soil pH was lower under the P. patula (pH 4.8-5.2) and E. grandis plantations (pH 4.6-5.3) when compared with the soil under the adjacent grasslands (pH 5.3-5.6). The soil under the P. patula plantations had lower exchangeable base cation concentrations (204-300 mg kg-1) compared with the adjacent Driekop grassland (452-645 mg kg-1), while there were no clear trends when comparing the exchangeable basic cation concentrations in the E. grandis plantations and the adjacent Brooklands grassland. The soil aluminium saturation was similar between the plantations, ranging between 39.7 and 63.3% in the P. patula plantations and 27.4 and 75.6% in the E. grandis plantations. The grasslands had lower soil aluminium saturations, ranging between 17.7 and 35.7% in the Driekop grassland and 17.5 and 39.1% in the Brooklands grassland. Exudates from mycorrhizal associations chelate iv acidic cations, rendering plantations more tolerant of acidic soils. Soil total nitrogen, phosphorus and carbon in the plantations were similar to the adjacent grasslands. Higher nitrogen mineralisation rates in the P. patula plantations corresponded with higher litter and foliar total N concentrations relative to the adjacent Driekop grassland, while there were no clear trends when comparing rates of N mineralisation in the E. grandis plantations and the Brooklands grassland. The concentrations of foliar amino acids and protein were significantly higher (p<0.01) in the plantation foliage, when compared the grassland foliage. The amino acid arginine, occurred in higher proportions relative to other amino acids in plantation foliage, when compared with the relative proportions found in the grasslands. Relative to nitrogen, foliar ratios indicated that phosphorus and potassium were the most limiting nutrients for the plantations, which possibly resulted in N storage in the form of protein and amino acids. The soil and foliage were the more sensitive indicators of the nutrients status than litter. Differences in nutrients concentrations between the lower and upper canopies in both plantations were minor and no major trends were found. Therefore the sub division of lower and upper canopies is not recommended for further foliar nutrient analyses. Age related trends were unclear as a result of higher soil clay contents found in the 13 year old P. patula plantation and the 8 year old E. grandis plantation as well as the various silvicultural practices implemented. In terms of the relative impacts of P. patula and E. grandis plantations, the results of this study imply that the upper 20cm of soil was more impacted under the P. patula plantations than under the E. grandis plantations, in terms of reduced base cation and increased acid cation concentrations. The P. patula plantations are shallow rooters and thus the base cation status of the upper soil horizons may affect the sustainability of the P. patula plantations.

Orientador: Carlos Frederico Wilcken / Resumo: Os afídeos do gênero Cinara (Hemiptera: Aphididae) são importantes pragas de coníferas em diversos países. No Brasil o pulgão do Pinus foi detectado pela primeira vez em 1996 em plantio de Pinus taeda no estado de Santa Catarina, sendo C. atlantica registrada no estado de São Paulo em 1999. Atualmente esta espécie vem causando perdas em plantações comerciais de Pinus spp.. Este trabalho teve por objetivos: 1) Avaliar o efeito do manejo de plantas invasoras na população de C. atlantica e seus inimigos naturais em P. taeda. 2) Estudar a biologia e o potencial de predação das joaninhas sobre C. atlantica. O experimento foi instalado em área de 17,7 ha, com plantio de P. taeda de 6 meses em Buri, SP. O delineamento experimental foi em blocos casualizados com quatro tratamentos e quatro repetições. Em cada parcela foram amostradas 40 plantas, dispostas em quatro amostras de 10 plantas em linha. Os seguintes tratamentos de controle de plantas invasoras foram aplicados nas entrelinhas: a) presença de plantas invasoras (sem controle); b) manejo com roçadeira; c) manejo com gradagem; e d) manejo com herbicida. A infestação pelo pulgão do Pinus foi avaliada pela população de pulgões, por escala de notas e número de colônias/planta. Também foram atribuídas notas para a determinação dos danos. Foram realizadas 16 avaliações mensais (maio/2001 a agosto/2002), pelas quais pôde-se constatar maior população de C. atlantica nos tratamentos onde as plantas invasoras foram controladas totalmente devido ao sistema de manejo aplicado. A maior infestação foi constatada no manejo com uso de herbicida. O manejo por roçada apresentou menor número de plantas atacadas. Os danos de maior... (Resumo completo, clicar acesso eletrônico abaixo). / Abstract: Aphids of genus Cinara (Hemiptera: Aphididae) are important conifer pests in several countries. In Brazil, pine aphid was detected for the first time in 1996 in planting of Pinus taeda in State of Santa Catarina, with C. atlantica registered in State of São Paulo in 1999. Nowadays this species causes losses in pine plantations. This work had as objectives: 1) evaluate the effects of weed management in C. atlantica population and its natural enemies in P. taeda; 2) study the ladybugs biology and their predation potential on C. atlantica. The experiment was carried out in an area of 17.7 ha, with P. taeda plantation of 6 months age in Buri, SP. The experimental design was in randomized blocks with four treatments and four repetitions. In each block were sampled 40 plants, distributed in four samples of 10 plants in line. The following weed control treatments were applied in interlines: a) weed presence (no control); b) management with brush cutter; c) management with disk harrow; and d) management with herbicide. Aphid population evaluated infestation by notes scale and by colonies/plant numbers. It was also attributed notes for damage evaluation. It was accomplished 16 monthly evaluations (May, 2001 to August, 2002), by which ones could verify larger population of C. atlantica in treatments where weed was controlled totally (disk harrow and herbicide). The largest infestation was verified in herbicide management areas. The brush cutter management presented smaller number of attacked plants. The main damages caused by aphids in pine plants were shoot bifurcation and multishooting. P. taeda growth was above of the average in the system with weed in interlines. Regarding the C. atlantica natural enemies evaluations, it was considered for the predators of families Coccinellidae, Chrysopidae and Syrphidae. In interlines, it was verified predators... (Complete abstract, click electronic address below). / Mestre

No Brasil, foram introduzidas diversas espécies de Pinus e a espécie P. elliottii Engelm apresenta o comportamento invasor em unidades de conservação de Cerrado, no estado de São Paulo. O objetivo deste estudo foi comparar P. elliottii com outras duas espécies do mesmo gênero, P. caribaea Moret e P. oocarpa Shiede ex Schltdl, através de: viabilidade e germinabilidade das sementes, em laboratório; sobrevivência das plântulas logo após a germinação e acompanhamento de seu crescimento em estufa; germinabilidade em fisionomias campestres; e chuva de sementes dentro de talhões. Para P. elliottii, também foi verificada a distância de dispersão das sementes. As sementes utilizadas nos testes em laboratório foram coletadas em Águas de Santa Bárbara (Estação Ecológica de Santa Bárbara - EEcSB; Floresta de Águas de Santa Bárbara - FASB) e Itirapina (Estação Experimental de Itirapina - EExI). Primeiramente, as sementes foram colocadas em água para separação das sementes cheias das vazias (flutuabilidade). Em seguida, as sementes cheias foram testadas quanto à viabilidade por meio de sal de tetrazólio e quanto à germinabilidade. As sementes germinadas foram acompanhadas até os cotilédones ficarem visíveis e uma parte destas teve o crescimento acompanhado até a 24ª semana. A germinabilidade em fisionomias campestres foi realizada apenas na EEcSB, onde as sementes das três espécies foram semeadas em diferentes graus de umidade do solo. Coletores de sementes foram instalados dentro dos talhões de Pinus spp., tanto na EEcSB-FASB como na EExI, para quantificar a chuva de sementes. A distância de dispersão das sementes de P. elliottii foi avaliada apenas na EEcSB, durante o período de maior dispersão de sementes (março a maio), e teve como classes de distâncias em relação ao talhão: 10, 30, 60, 100, 150 e 250 m. Os resultados dos testes em laboratório mostraram que P. caribaea e P. elliottii apresentam as maiores proporções de sementes cheias (>70%) e P. oocarpa e P. elliottii, as maiores taxas de viabilidade (>90%) e germinabilidade (>90%). Dentre as sementes germinadas das três espécies, mais de 90% desenvolveram-se até o aparecimento dos cotilédones. Quanto ao crescimento, P. caribaea e P. oocarpa apresentaram maior vigor em relação a P. elliottii. Em campo, as três espécies apresentaram, de maneira similar, baixíssima porcentagem de germinação (<1,5% do total semeado), preferencialmente em solos mais úmidos. A chuva de sementes de P. elliottii dentro de talhões foi muito maior (pelo menos o dobro) em comparação com as outras duas espécies. A distância de dispersão das sementes de P. elliottii foi maior nos primeiros 30 m, mas chegou até os 150 m. Portanto, P. caribaea e P. oocarpa apresentaram condições de se tornarem invasores pela viabilidade de suas sementes, vigor de crescimento e germinação em campo, mas o fator determinante para o sucesso na invasão P. elliottii é, provavelmente, a pressão de propágulos, maior do que as outras duas espécies, causada pela sua alta produção de sementes e intenso plantio. / Several pine species of the genus Pinus have been introduced in Brazil, and P. elliottii Engelm is presently considered an invasive species in the Brazilian savanna (Cerrado) in São Paulo State. The aim of this study was to compare P. elliottii with two other Pinus species, P. caribaea Moret and P. oocarpa Shiede ex Schltdl according to: seed viability and germinability, in laboratory; survival of seedlings soon after germination and their growth, in greenhouse; germination at field conditions; and seed rain inside the plantation. The distance of seed distance dispersal was also verified for P. elliottii. The seeds for the laboratory tests were collected in Águas de Santa Bárbara (Santa Barbara Ecological Station - EEcSB; Águas de Santa Bárbara Forest - FASB) and Itirapina (Itirapina Experimental Station - EExI). First, the seeds were placed in water to separate the full from the empty seeds (flutuability). Then full seeds were tested by viability (tetrazolium) and germinability. Germinated seeds were monitored until the cotyledons were visible and some of those were monitored up the 24th week to verify their growth rate. In the EEcSB, seeds of the three species were sown at different levels of soil moisture to observe the germination in the field. In order to quantify the species seed rain seed collectors were installed inside the Pinus spp. stands, in both EEcSB-FASB and EExI. The distance of P. elliottii seed spread was evaluated only at the EEcSB, during the greater period of seed dispersion (March to May), at the distances of 10, 30, 60, 100, 150 and 250 m from the planted areas. The results of laboratory tests showed that P. caribaea and P. elliottii have the highest proportions of full seeds (> 70%) and P. oocarpa and P. elliottii, the highest viability (> 90%) and germination rates (> 90%). Among the germinated seeds of the three species more than 90% developed to seedling stage. Concerning seedling growth, P. caribaea and P. oocarpa showed greater vigor than P. elliottii. In the field, the three species showed similar and very low germination rates (<1.5% of the total sown), preferably in more humid soils. The seed rain of P. elliottii inside the plantation stand was much higher (at least twice) compared to the other two species. The dispersal distance of P. elliottii seeds was higher in the first 30 m, but reached 150 m. The results show that P. caribaea and P. oocarpa are able to become invaders due to their high values of seed viability and germination, and vigor growth, however, the determining factor for the invasive success of P. elliottii is probably its higher propagule pressure, caused by several human mediated introductions, and high production of seeds.