This thesis studied the performance of the thermal hydraulic software TRACE applied on an intermediate sized break (IB) happening on the cold leg in a pressurized water reactor (PWR), causing a loss-of-coolant accident (LOCA). The same accident has previously been simulated in the PKL Test Facility, which is a scaled version of a PWR and is used to simulate transients stemming from different accidents. The thesis was performed as a semi-blind study: firstly, the accident was simulated without any knowledge of the PKL results. When a final blind model was chosen, the PKL results were revealed, and the TRACE model was improved. Before the simulations of the IB-LOCA took place, the new internal parts in the upper parts of the reactor pressure vessel in PKL had to be modelled, and the steady state had to be tuned to attain the correct initial conditions. The simulations were performed by using the software SNAP together with TRACE, providing a graphical interface. TRACE achieved steady state with satisfying results regarding water levels, pressure losses and mass flows. The temperatures in TRACE deviated from the PKL temperatures but an explanation is uncertainties in PKL. To verify TRACE’s core output power, the calculation of the power was done by using mass flow rate and specific entropy and comparing to the heaters’ specified power. This resulted in lower output power meaning that the coolant was not heated enough. This indicated non-physical energy losses in the TRACE model and should be further investigated.The blind transient simulation, modelled with default choked flow and no offtake model, resulted in TRACE overestimating the break mass flow and the peak cladding temperatures, compared to the PKL reference solution. This resulted in the pressure decreasing too quickly and too early activation of the safety system. The modified simulations showed that it is important that the offtake model, which accounts for different flow regimes, is activated. Default choked flow multipliers were the multipliers that performed the best. However, none of the transient simulations could be completed due to fatal errors and memory problems, but some conclusions could be drawn from the observed trends. This concluded in the offtake model being most important due to stratified flow occurring.

No description available.

Optical membranes are currently pursued for their ability to replace the conventional mirrors that are used to correct wave front aberration and space-based telescopes. Among some of the many benefits of using optical membranes, is their ability to considerably reduce the weight of the structure. As a secondary effect, the cost of transportation, which is of great interest in space applications, is reduced as well. Given the low density of these thin-film membranes, the lower end dynamics play a greater significant role than their rigid plate-like counterparts in achieving functional mirrors. Space-based mirrors are subjected to a series of disturbances. Among those encountered are thermal radiation, debris impact, and slewing maneuvers. Thus, dynamic control is essential for the adequate performance of thin-film membrane mirrors. With this in mind, the work described herein aims to improve the performance of optical membranes with an innovative, acoustical control approach to suppress vibration of optical membranes backed by an air cavity. This is achieved by using a centralized acoustic source in the cavity as the method of actuation. The acoustic actuation is of great interest since it does not mass load the membrane in the conventional way, as most methods of actuation would. To achieve this end goal, two structural-acoustic coupled models are developed to describe the dynamics of a pressurized optical membrane system. This is done through an impedance based modeling approach where the subsystems are modeled individually, and then coupled at the interface. The control of the membrane is implemented using a positive position feedback approach. The theory is also extended to positive velocity and positive acceleration feedback. Three experiments are carried out to validate the models previously mentioned. Successful implementation of a control experiment is also accomplished leading to considerable attenuations in the coupled membrane's dynamics. / Ph. D.

A fuel burnup model of a Pressurized Water Reactor (PWR) was developed based on one-group diffusion theory and used simple thermal cross sections. A computer program which simulates the depletion of the core of a PWR was written based on this model. The basic idea was to develop a fuel depletion program which could be readily understood by nuclear engineering students. Thus, accuracy was sacrificed for the sake of simplicity. The model was based upon a typical PWR with three concentric regions in the radial direction of differing fuel enrichment. Each of the regions was homogenized and the concentrations of the isotopes in each region were considered constant over a time interval. The isotopes considered were U-235, Pu-239, U-238, Xe-135, I-135, Sm-149, Pm-149 and the lumped burnable poison isotope. The flux was approximated by the sum of two trigonometric functions. The magnitude and shape of the flux were determined by holding power constant, constraining system to be critical and varying the soluble boron concentration to find the fla~test possible positive flux. A flux magnitude computed in this manner was compared to a similar flux magnitude given in a Final Safety Analysis Report. The concentrations of the isotopes were determined from the differential equations describing the rate of change of the concentrations. The behavior of the isotopes over core life was graphed and wherever possible compared to graphs from other sources. The concentrations calculated for U-235, U-238 and Pu-239 after 450 days were compared to the concentrations of the same isotopes calculated by a zero dimensional three-group model. The percentage difference between the concentrations determined by the two models varied from about 69% for Pu-239 to 1% for U-238. / Master of Science

The presence of deposits on the secondary side of pressurized water reactor (PWR) steam generator systems is one of the main contributors to the high maintenance costs of these generators. Formation and transport of corrosion products formed due to the presence of impurities, metals and metallic oxides in the secondary side of the steam generator units result in formation of deposits. This research deals with understanding the deposit formation and characterization of deposits by studying the samples collected from different units in secondary side system at Comanche Peak Steam Electric Station (CPSES). Fourier transform infrared spectrophotometry (FTIR), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) have been used for studying the phases, morphologies and compositions of the iron oxides formed at Unit 1 and Unit 2 of secondary side of steamgenerator systems. Hematite and magnetite were found to be the dominant phases of iron oxides present in the units. Fe, Cr, O, Ni, Si, Cl and Cu were found in samples collected from both the units. A qualitative method was developed to differentiate iron oxides using laser induced breakdown spectroscopy (LIBS) based on temporal response of iron oxides to a high power laser beam. A quantitative FTIR technique was developed to identify and quantify iron oxides present in the different components of the secondary side of the steam generator of CPSES. Amines are used in water treatment to control corrosion and fouling in pressurized water reactors. CPSES presently uses an amine combination of dimethylamine (DMA), hydrazine and morpholine to control the water chemistry. Along with the abovementioned amines, this study also focuses on corrosion inhibition mechanismsof a new amine DBU (1, 8-diazabicyclo [5.4.0] undec-7-ene). Electrochemical impedance spectroscopy and polarization curves were used to study the interaction mechanism between DBU solution and inconel alloys 600 and 690 at steamgenerator operating temperatures and pressures. Of all the amines used in this study (DMA, DBU, ETA, and morpholine), DMA was more effective at keeping the passive film formed on the alloy 600 surface from failing at both ambient and high temperatures. Morpholine was found result in higher corrosion resistance compared to the other amines in case of alloy 690.

En cas d’accident impliquant des produits chimiques, il peut être fait appel à des experts pour évaluer les effets générés par cet accident. Ces experts fournissent des distances d’effets à l’aide de modélisations informatiques et sont confrontés à une difficulté majeure : peu d’éléments à leur disposition pour caractériser la situation.L’objectif de cette thèse est de proposer une méthodologie permettant de prendre en compte les incertitudes relatives aux données d’entrée dans les modélisations effectuées en situation d’urgence tout en restituant de manière explicite cette incertitude au gestionnaire de la situation d’urgence.Une première phase a consisté à évaluer, pour une situation à même de générer un nuage toxique ou explosible, la dispersion des résultats des modélisations. Il a été établi une hiérarchisation des variables d’entrée en fonction de leur influence sur le résultat final. Cette phase a été réalisée au moyen d’une analyse de sensibilité dont la stratégie a été spécifiquement développée.Une seconde phase a eu pour but d’établir une méthodologie d’estimation des distances d’effets en situation d’urgence en tenant compte du niveau d’incertitude des variables d’entrée. Une méthodologie de classification opérationnelle des données d’entrée a été réalisée. Elle s’appuie sur deux critères : la sensibilité du modèle au paramètre d’entrée et l’incertitude sur sa valeur (imprécision ou variabilité). Sur cette base, une nouvelle manière d’utiliser ces variables a été proposée. Enfin, différentes façons de restituer de manière opérationnelle les résultats des modélisations ont été proposées. / During accidents involving chemicals, experts can be asked to assess the effects generated. These experts provide distance effects using computer modeling and are faced with a major difficulty: little (or no) information available in order to assess the situation.The objective of this thesis is to suggest a methodology able to take into account uncertainties in the input data for the modeling carried out in emergency situations and to return explicitly these uncertainties to the manager of the crisis.A first step was to evaluate, for a given situation generating a toxic or flammable cloud, the dispersion of modeling results. A ranking of the input variables according to their influence on the final result was established. This phase was carried out on the basis of a sensitivity analysis with a specifically developed strategy.A second phase aimed to establish a methodology for estimating distance effects (in crisis situations), which takes into account the level of uncertainty in the input variables. A methodology for the classification of input operational data was carried out. This methodology is based on two criteria: the sensitivity of the model to the input parameter and the uncertainty about its value (imprecision or variability). On this basis, a new way of using these variables was suggested. Finally, several methods aimed to restore explicitly the results of this modeling were suggested.

Enquanto as amêndoas de babaçu são conhecidas por fornecer óleo rico em zinco, fósforo e potássio, seu mesocarpo possui propriedades anti-inflamatórias ainda pouco exploradas. Este estudo visou determinar a composição físico-química e o potencial de toxicidade in vivo do óleo de amêndoas de babaçu e da farinha de seu mesocarpo (ambos extraídos com CO2 supercrítico) bem como avaliar a atividade anti-inflamatória do mesocarpo (extraído com etanol pressurizado). Os extratos foram obtidos via técnicas inovadoras de extração que evitam a degradação de compostos termossensíveis e os resultados aqui alcançados apontam para a composição de extratos obtidos via tecnologia verde e sua caracterização. A extração com fluido supercrítico (SFE) é uma tecnologia limpa (pois não emprega solventes orgânicos) e promissora para obter extratos enriquecidos em compostos com atividade biológica como, por exemplo, fitosterois. Por sua vez, a extração com líquido pressurizado (PLE) é considerada rápida, eficiente e seletiva, sendo este trabalho o primeiro a isolar os flavonoides do extrato etanólico do mesocarpo de babaçu extraído via PLE. Os resultados indicaram que os maiores rendimentos de extração do óleo foram de 55,34 g/100g usando álcool isopropílico como solvente pressurizado e de 53,12 g/100g usando etanol. Tais rendimentos representam 92,4 e 88,7% do total de óleo nas sementes, respectivamente. Rico em ácidos láurico, mirístico, oleico e palmítico, o perfil de ácidos graxos foi o mesmo para os dois solventes nas condições operacionais utilizadas. Com uso de álcool isopropílico, o extrato apresentou a maior concentração de β-sitosterol mediante 3 min de contato com o solvente (St) e 66% de volume de solvente (≅ 7,48 mL, SV). Com uso de álcool etílico, a maior concentração de β-sitosterol ocorreu para 3 min de tempo de contato (St) e 94% (≅ 10,25 mL) de volume de solvente (SV). Em função da composição de ácidos graxos, os principais TAGs presentes no óleo foram COL, LOL, LOM, LOP e MOP. Na SFE, os maiores rendimentos do óleo de babaçu variaram de 51,94 a 57,71 g/100g (para 25 MPa) e de 53,65 a 59,93 g/100g (para 35 MPa), para temperaturas de 40, 50, 60, 70 e 80 °C. A maior concentração de β-sitosterol (27,43 mg/100g de óleo) foi obtida na extração a 70 °C e 25 MPa. Os extratos do mesocarpo de babaçu também foram obtidos via PLE com etanol pressurizado. O maior rendimento (2,95%) e a maior concentração de fenólicos totais (1.257,25 mgGAE/100g) de pó dos extratos do mesocarpo de babaçu foram obtidos a 86 °C e 3 min (St). A maior atividade antimicrobiana na inibição de Staphylococcus aureus ocorreu extrações com menores tempos de contato (St) e elevadas temperaturas (na faixa supra), condições que também propiciaram os melhores rendimentos de extração e atividade antioxidante. O estudo de toxicidade foi feito para o óleo de amêndoas de babaçu obtido via SFE. Na dose aguda de 2.000 mg/kg e no estudo subagudo nas doses de 1, 3 e 5 mg/kg de óleo (doses repetidas), os resultados indicaram que este óleo não apresenta letalidade aos animais. Para avaliar a atividade anti-inflamatória foi utilizado o extrato do mesocarpo de babaçu obtido com etanol pressurizado a 86 °C e 3 min (St). Tal atividade foi confirmada para a dose de 30 mg/kg, administrada em ratos com processo inflamatório induzido com carragenina a 3%. Embora tenham sido constatadas a baixa toxicidade do óleo e a atividade anti-inflamatória do extrato do mesocarpo, mais estudos de avaliação toxicológica são necessários para aplicações do óleo de amêndoas e do extrato de mesocarpo de babaçu em produtos farmacêuticos, alimentícios e/ou cosméticos. / While babassu almonds are known for providing oil rich in zinc, phosphorus and potassium, its mesocarp has anti-inflammatory properties yet underexplored. The present study aimed at determining physical-chemical composition and in vivo toxicity potential of babassu almonds oil and its mesocarp flour (both extracted with supercritical CO2) as well as anti-inflammatory activity of babassu mesocarp (extracted with pressurized ethanol). Extracts were obtained using innovative extraction techniques that avoid the degradation of thermosensitive compounds and results here obtained point to the composition of extracts obtained via green technology and their characterization. Supercritical fluid extraction (SFE) is a promising clean technology (as organic solvents are not employed) to obtain extracts enriched in compounds with biological activity (e.g. phytosterols). In its turn, pressurized fluid extraction (PFE) is considered fast, efficient and selective, this work being the first to isolate flavonoids from ethanolic extract of babassu mesocarp extracted via PLE. Results indicated that highest extract yields of babassu oil were 55.34 g/100 g with isopropyl alcohol as pressurized solvent and 53.12 g/100 g with ethanol as solvent. Aforesaid extracts respectively represent 92.4% and 88.7% of total oil in seeds. Rich in lauric, myristic, oleic and palmitic acids, the fatty acid profile was the same for the two solvents and applied operating conditions. Using isopropyl alcohol, extract had the highest β-sitosterol concentration for 3 min contact time with solvent (St) and 66% of volume of solvent, ≅ 22.44 mL (SV). Using ethyl alcohol as solvent, highest β-sitosterol concentration occurred for St = 3 min and SV = 94% ≅ 31.96 mL. According fatty acids composition, main TAGs present in oil were COL, LOL, LOM, LOP and MOP. In SFE, highest yields of babassu oil ranged from 51.94 to 57.71 g/100 g (at 25 MPa) and from 53.65 to 59.93 g/100 g (at 35 MPa) at temperatures of 40, 50, 60, 70 and 80 °C. The highest concentration of β-sitosterol (namely, 27.43 mg/100g-oil) was obtained for extraction at 70 °C and 25 MPa. Babassu mesocarp extracts were also obtained via PLE with pressurized ethanol. The highest yield (2.95%) and the highest total phenolic concentration (1,257.25 mgGAE/100g) of babassu mesocarp extract were obtained at 86 °C and 3 min (St). The highest antimicrobial activity in Staphylococcus aureus inhibition occurred for lower contact time (St) values and higher temperatures (in aforesaid values), which were conditions also providing the best extraction yields and antioxidant activity. Toxicity study was carried out for babassu almond oil obtained via SFE. At the acute dose of 2,000 mg/kg and in subacute dose study at 1, 3 and 5 mg/kg of oil (repeated doses), results indicated that this oil did not present lethality in animals. Anti-inflammatory activity was evaluated by using babassu mesocarp extracted with pressurized ethanol at 86 °C and 3 min (St). Such activity was confirmed at 30 mg/kg dose administered in rats with induced inflammatory process with carrageenan at 3%. While low toxicity of oil and anti-inflammatory activity of mesocarp extract were both observed, further evaluation studies are required for applications of almond oil and babassu mesocarp extracts in pharmaceutical, food and/or cosmetic products.

Les réacteurs nucléaires de génération III s'inscrivent dans la continuité des réacteurs à eau sous pression actuels, tout en présentant un certain nombre d'améliorations en terme de sûreté, de rendement et d'environnement.Parmi les caractéristiques de ces réacteurs, la taille importante du coeur et l'utilisation d'un réflecteur lourd se traduisent par une meilleure efficacité neutronique et une meilleure protection de la cuve.Du fait de leur grande taille, le risque de basculement de la nappe de puissance neutronique est exacerbé. Le basculement est donc un paramètre d'intérêt à prendre en compte dans les études de sûreté. Par ailleurs, le calcul de l'incertitude associée à la nappe de puissance neutronique est difficilement atteignable par les méthodes déterministes actuellement implémentées dans les codes de neutronique.Ce travail de thèse a donc porté sur le développement d'une méthode stochastique innovante de propagation des incertitudes neutroniques. Tout en étant basée sur des résultats probabilistes, elle tire parti de la puissance croissante des moyens de calcul informatique afin de parcourir tous les états du réacteur statistiquement prévus.Après avoir été validée, cette méthode a été appliquée à un benchmark de grand coeur de l'OCDE/AEN avec des valeurs de covariances issues d'une analyse critique. Ainsi, pour ce système, l'incertitude associée au facteur de multiplication effectif des neutrons keff $(1\sigma)$ vaut 638 pcm . Par ailleurs, le basculement total vaut 8.8 \% $(1\sigma)$, et l'incertitude maximale associée à l'insertion d'un groupe de barres absorbantes utilisées pour son pilotage vaut 11~\% $(1\sigma)$. / Generation III Light Water Reactors undoubtedly follow design guidelines comparable to those of current PWRs. Furthermore, they take advantage of enhanced features in terms of safety, energy efficiency, radiation protection and environment. Then, we talk about an evolutionary approach. Amongst those improvements, the significant size and the use of a heavy reflector translate into a better neutronics efficacy, leading to intrinsic enrichment benefits then to natural uranium profits. They contribute to the core vessel preservation as well.Because of their large dimensions, the neutronic bulge of this kind of reactors is emphasized. Therefore, it is a parameter of interest in the reactor safety studies. Nevertheless, the uncertainty related to the radial power map is hardly reachable by using the numerical methods implemented in the neutronics codes.Given the above, this PhD work aims to develop an innovative stochastic neutronics uncertainties propagation method. By using recent probabilistic results, it makes good use of the growing calculation means in order to explore all the physical states of the reactor statiscally foreseen.After being validated , our method has been applied to a reactor proposed in the framework of a large core OECD/NEA international benchmark with carefully chosen covariances values. Thus, for this system, the uncertainties related to the keff reaches 638~pcm $(1\sigma)$. What is more, the total bulge equals 8.8~\% $(1\sigma)$ and the maximal uncertainty related to the insertion of a group of control rods reaches 11~\% $(1\sigma)$.

Certains éléments des internes de cuve des réacteurs à eau pressurisée (REP) en acier inoxydable austénitique 316L, présentent un endommagement prématuré par corrosion sous contrainte assistée par l’irradiation. Ce phénomène est complexe puisqu’il implique le couplage entre le matériau lui-même, l'état de contrainte, l’irradiation et l’environnement. L’irradiation neutronique couplée à un facteur environnemental, l’hydrogène, pourrait jouer un rôle dans ce phénomène. Ainsi, les travaux présentés dans ce manuscrit porte sur l’étude des effets des défauts induits par l’irradiation sur le piégeage de l’hydrogène dans un acier inoxydable austénitique 316L au cours de son oxydation en milieu primaire des REP. Grâce à des implantations ioniques permettant de reproduire le même type de défauts que ceux induits par les neutrons, les interactions hydrogène - défauts ont tout d’abord été étudié avec une approche modèle par chargement cathodique en deutérium, traceur isotopique de l’hydrogène. Cela a permis de mettre en évidence le piégeage de cet élément au niveau des défauts induits par l’implantation ionique et particulièrement des cavités. Un modèle de résolution numérique des équations de McNabb et Foster permettant de simuler la diffusion et le piégeage de l’hydrogène dans un matériau a confirmé ce résultat. Ensuite des essais d’oxydation en milieu primaire simulé des REP à 320 °C ont été réalisés afin de comparer les couches d’oxyde formées entre matériaux implantés et non implantés, ainsi que leur prise d’hydrogène. Ces essais ont permis de mettre en avant le piégeage de l’hydrogène dans l’alliage sous l’oxyde et, dans une moindre mesure, au niveau des défauts d’implantation plus en profondeur. / Some components of vessel internals in pressurized water reactor (PWR) made of stainless steel, have shown cracks induced by Irradiation-Assisted Stress Corrosion Cracking (IASCC). This complex phenomenon originates from the coupling between the material itself, a tensile stress state, environmental conditions and irradiation. This PhD thesis aims at studying the influence of hydrogen in IASCC and particularly its interactions with the defects created by neutron irradiation in a 316L austenitic stainless steel. Thanks to ion implantation, defects similar to neutron irradiation-induced defects were created. As a first step, hydrogen - defects interactions were studied with a model approach consisting in deuterium cathodic charging. Deuterium was used as an isotopic tracer for hydrogen. This technique allowed to highlight hydrogen trapping by implantation-induced defects (mostly by the cavities) in 316L stainless steel. Simulation of hydrogen diffusion and trapping in the studied materials with a numerical resolution model of McNabb and Foster’s equations confirmed the experimental results. Then, oxidation tests were performed in PWR simulated primary environment at 320 °C in order to study the effects of irradiation-induced defects on the oxidation and the hydrogen uptake of the 316L stainless steel. The major highlight of these experiments was the observation of hydrogen accumulation in the alloy beneath the oxide, due to trapping by vacancies created by oxidation process and by ion implantation. In addition, hydrogen trapping was observed deeper in the alloy and it was attributed to the cavities induced by implantation.

Este projeto de pesquisa, com o apoio dos pequenos produtores de café do Estado de São Paulo/Brasil, visou estudar e caracterizar o óleo de grãos de café verde obtido pela extração supercrítica e extração com fluido pressurizado. Foi feita a caracterização deste produto, considerando os componentes minoritários com atividade biológica demonstrada na literatura, como os componentes fenólicos e os diterpenos cafestol e kahweol. Com o objetivo de obter o óleo de café verde enriquecido, principalmente nestes dois principais diterpenos, componentes relevantes para as indústrias cosmética e farmacêutica, aplicaram-se como inovação tecnológica a extração supercrítica (SFE) e extração com fluido pressurizado (PFE). Os ensaios de extração foram realizados no Laboratório a Alta Pressão e Produtos Naturais (FZEA/USP/Brasil) para as SFE e no Laboratoire de Procédés (IUT/Université d\'Orléans/França) para PFE. No desenvolvimento da metodologia de análise dos diterpenos foram realizados estudos do método de saponificação do óleo de café verde Arabica (Coffea arabica) cv. Catuaí amarelo, obtido por prensagem (OP). E, concomitantemente a este estudo analítico, foram feitas as extrações do óleo dos mesmos grãos de café verde via SFE e PFE. Os diferentes óleos de grãos de café verde, obtidos pelos três métodos de extração foram analisados e comparados entre si considerando principalmente a concentração dos diterpenos. A metodologia analítica desenvolvida na primeira fase do trabalho constatou que para uma melhor análise dos diterpenos a reação de saponificação deve ser feita utilizando NaOH e a 70 °C. A fração insaponificável foi analisada para os diferentes extratos. Para os extratos obtidos via SFE, a cafeína teve que ser separada do óleo. A análise de quantificação dos diterpenos via cromatografia gasosa, utilizando o próprio cafestol e kahweol como padrão externo, indicou que o óleo obtido por SFE apresentou 50.180,41 mg cafestol/kg óleo e 63.764,32 mg kahweol/kg óleo na condição otimizada. Para a PFE as concentrações máximas obtidas foram de 65.876,29 mg cafestol/kg óleo e 53.328,47 mg kahweol/kg óleo e, no óleo de café verde obtido pelo método convencional a concentração dos diterpenos mostrou-se inferior com 7.508 mg cafestol/kg óleo e 12.822 mg kahweol/kg óleo. As propriedades físicas medidas visando a caracterização do óleo dos grãos de café verde obtido por SFE e prensagem dos grãos indicaram que tanto a densidade quanto a viscosidade diminuem com o aumento da temperatura. Para ambos os óleos, os comportamento da densidade foi semelhante, para aquele obtido pelo método convencional, os valores variaram de 0,94177 g/cm3 (25 °C) a 0,92143 g/cm3 (55 °C) e para o óleo obtido por SFE a variação foi de 0,93645 g/cm3 (25 °C) a 0,9157 g/cm3 (55 °C). A viscosidade dinâmica para o óleo obtido por prensagem variou de 127,8798 mPa×s (25 °C) a 35,0510 mPa×s (55 °C) e, para o óleo obtido por SFE, estes valores foram menores, entre 84,0411 mPa×s (25 °C) e 24,2555 mPa×s (55 °C). O índice de refração para ambos os óleos foi semelhante. Na extração com fluido pressurizado, (PFE), um valor ótimo de rendimento do extrato foi obtido quando se empregou metanol como solvente para uma temperatura de 100 °C e tempo de equilíbrio estático de 20 min. Este valor foi 183% superior a media dos resultados obtidos nos outros ensaios. Na concentração de diterpenos nestes extratos, nota-se também que o etanol foi menos eficiente que o metanol. Na comparação dos dois processos inovadores (SFE e PFE), a concentração de kahweol apresenta-se como o principal componente em quase todos os ensaios, com valores da relação cafestol/kahweol entre 0,73 e 0,96. No entanto, ao ser analisado o processo individualmente, percebe-se que a extração com fluido supercrítico indica que a maioria dos extratos obtidos teve uma concentração de kahweol superior ao cafestol. / This research project, with the support of small coffee producers in the State of São Paulo/Brazil, aimed to study and characterize the green coffee oil obtained by the supercritical fluid extraction and pressurized fluid extraction. Was made to characterize this authentic product, considering the minor components with biological activity demonstrated in the literature, such as phenolic compounds and diterpenes cafestol and kahweol. In order to get the green coffee oil enriched, mainly in these two major diterpenos, components relevant to cosmetic and pharmaceutical industries, applied technological innovation as the supercritical fluid extraction (SFE) and pressurized fluid extraction (PFE). The extraction tests were performed at the High Pressure Laboratory and Natural Products (FZEA/USP/Brazil) for SFE and the Laboratoire de Procédés (IUT/Université d\' Orléans/France) for PFE. In the development of methodology for analyzing diterpenes were conducted studies on the method of saponification of the green oil Arabica coffee (Coffea arabica) cv. yellow Catuaí, obtained by pressing (OP). And, concomitantly this analytical study were extracted from the oil of the same green coffee beans by SFE and PFE. Different green coffee beans oils, obtained by the three extraction methods were analyzed and compared with each other considering mainly the concentration of diterpenes. The analytical methodology developed in the first phase of the work found that for a better analysis of the diterpenes saponification reaction must be made using NaOH and 70 °C. The unsaponifiable fraction was analyzed for the different extracts. Specifically for extracts obtained by SFE, caffeine had to be separated from the oil. The analysis and quantification of diterpenes made by gas chromatography, using himself as cafestol and kahweol external standard, indicated that the oil obtained by SFE showed cafestol 50,180.41 mg/kg oil and kahweol 63,764.32 mg/kg oil in optimal condition. For the PFE maximum concentrations were obtained cafestol 65,876.29 mg/kg oil and kahweol 53,328.47 mg/kg oil and the green coffee oil obtained by the conventional method the concentration of diterpenes was lower than with 7,508 mg cafestol/kg oil kahweol and 12,822 mg/kg oil. The physical measures for the characterization of oil from green coffee beans obtained by SFE and pressing the grains indicated that both the density and the viscosity decrease with increasing temperature. For both oils, the density behavior was similar, to that obtained by the conventional method, the values ranged from 0.94177 g/cm3 (25 °C) to 0.92143 g/cm3 (55 °C) and the oil obtained by the SFE variation was 0.93645 g/cm3 (25 °C) to 0.9157 g/cm3 (55 °C). The dynamic viscosity for the oil obtained by pressing ranged from 127.8798 mPa×s (25 °C) to 35.0510 mPa×s (55 °C) and the oil obtained by SFE, these values were lower, between 84.0411 mPa×s (25 °C) and 24.2555 mPa×s (55 °C). The index of refraction for both oils was similar. In the extraction pressure, (PFE), an optimal value of the extract yield was obtained when methanol was used as solvent at a temperature of 100 °C and static equilibrium time of 20 min. This value was 183% above the average of the results obtained in other tests. At the concentration of these diterpenes extracts, note also that the ethanol was less effective than methanol. In comparing the two innovative processes (SFE and PFE), the concentration of kahweol presents itself as the main component in almost all tests, compared with values of cafestol/kahweol between 0.73 and 0.96. However, the process to be analyzed individually, it is clear that the supercritical fluid extraction indicates that most of the extracts had a concentration higher of kahweol than cafestol.