Thesis (Ph. D.)--University of Michigan, 1918. / "Parts I, II, and III are devoted to an exposition and criticism of Rudolf Haym's interpretation of the problem."--P. [v]. Bibliography: p. [ix]-x.

Thesis (Ph. D.)--University of Michigan, 1918. / "Parts I, II, and III are devoted to an exposition and criticism of Rudolf Haym's interpretation of the problem."--P. [v]. Bibliography: p. [ix]-x.

Thesis (M.S.)--University of Wisconsin--Madison, 1964. / eContent provider-neutral record in process. Description based on print version record. Bibliographical essay: leaves 168-176.

Nürtingen, FH, Diplomarb., 2004. / Betreuer: Winfried Otte.

Erlangen-Nürnberg, Universiẗat, Magisterarbeit, 2008.

Inaug.-Diss.--Erlangen. / Lebenslauf. Bibliography: p. [3]-4.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1984. / Typescript. Vita. Description based on print version record. Includes bibliographical references (leaves 232-240).

Thesis (Ph. D.)--University of Wisconsin, 1965. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

The overall aim of this thesis was to investigate the effect of preoperative exercise and training on postoperative outcome. Poor cardiorespiratory fitness has been associated with poor postoperative outcome including increased length of hospital stay and postoperative complications. Thus, increasing cardiorespiratory capacity prior to surgery via preoperative exercise training could potentially alter postoperative outcome. High intensity exercise training (HIIT) has been demonstrated to be an efficient training intervention to increase cardiorespiratory capacity in as little as 2 weeks. It was hypothesised that chronic preoperative exercise training (i.e. 2 weeks HIIT) would improve postoperative outcome measures (i.e. length of stay, complications and mortality) in urology cancer resection patients in comparison to a usual-care-only group (UC). Thirty-five urology cancer resection patients voluntarily enrolled into the study, of these thirty completed the study (15 UC, 15 EXP). There was a significant increase in length of stay (LOS) in the EXP group in comparison to the UC group (4.0 ±6.0 versus 3.0 ±1.5 days, P=0.03), respectively. However, after accounting for covariates (surgical severity, number of operations) LOS was not significantly different between groups (5.8 ±0.8 versus 5.0 ±0.8 days; P=0.24) for UC and EXP patients, respectively). There were no significant differences between groups for postoperative complications on days 1-8 post-surgery (P>0.05), despite significant differences between groups for VO2peak change data (-2.2 ±0.8 ml.kg-1.min-1 versus +1.3 ±0.8ml.kg-1.min-1; Eta2:0.24; P=0.02) for UC and EXP patients). Overall, two weeks preoperative HIIT does not appear to alter postoperative outcome in urology cancer resection patients. The effect of two weeks preoperative HIIT was investigated in colorectal cancer resection patients. It was hypothesised that chronic preoperative exercise training (i.e. 2 weeks HIIT) would improve postoperative outcome measures (i.e. LOS, complications and mortality) in colorectal cancer resection patients in comparison to the UC group. Twenty-one colorectal patients voluntarily enrolled into the study and completed the study (12 UC, 9 EXP). There were no significant differences between groups for LOS, (7.0 ±8.5 versus 6.0 ±2.0 days, Eta2:0.04; P=0.38) for UC and EXP patients, respectively). The Cox Regression hazard ratio was 1.55, suggesting that there was a 55% increased likelihood of being discharged on any given postoperative time point in the EXP group when compared to the UC group (95% CI: 0.25 to 1.65; P=0.36). There were no significant differences between groups for postoperative complications for days 1-10 post-surgery (P>0.05). Though, there was a moderate to large effect size for a reduction in postoperative complications on the 2nd (Eta2: 0.09), 4th and 8th postoperative day (Eta2: 0.07), in favour of the EXP group. There were no significant differences between groups for cardiorespiratory measures (i.e. AT, VO2peak) (P0.05). Thus, 2 hours hypoxia (O2: 14.5%) did not appear to significantly alter salivary stress markers. Therefore, the role of the cross-stressor adaptation hypothesis in exercise induced cardioprotection is unclear. The overall conclusion of this thesis is that preoperative exercise appears to improve postoperative outcome measures in AAA patients. However, the benefits of preoperative exercise training on postoperative outcome in colorectal and urology patients is equivocal. Though, there was a group effect on postoperative complications on days 2, 4 and 8 post-surgery in colorectal patients, in favour of the EXP group. Lastly, an acute bout of exercise did not appear to attenuate the stress response to a subsequent stressor.

Production of recombinant FVIII, the protein that is missing or dysfunctional in haemophilia A patients, is highly inefficient compared to other recombinant clotting factors such as FIX. This is predominantly due to complex intracellular trafficking, short half-life and protein instability. This study aimed to increase the amount of functional FVIII produced in mammalian cells by co-expression with anti-FVIII Camelid antibody fragments (VHH). Three VHH ligands were supplied by BAC BV (as DNA constructs), two of which when expressed in yeast are known to bind recombinant FVIII (ligands 2 and 7) and are used commercially as FVIII purification tools. From these three constructs, nine new VHH plasmids constructs were designed and transiently expressed in a stable BHK-human FVIII-expressing cell line. Of the nine VHH fragments that were co-expressed in the BHK FVIII cell line, four of these had a statistically significant impact on the ‘clotting time’ of the cell media as demonstrated by the activated partial thromboplastin time assay (aPTT). Two ligand 2 constructs (L2C1 and L2C2) prolonged the coagulation time by 4 seconds (P-value 0.0001, 95% confidence intervals 38.5-43.5), and 3.4 seconds (P-value 0.0072, 95% CI 36.5-40.3) respectively, indicating a decrease in functional FVIII activity versus media from the untransfected and null transfected BHK-FVIII cell line. Two ligand 7 constructs (L7C1 and L7C3) caused a decrease in coagulation time of 3.2 seconds (P=0.0057, 95% CI 30.5-33.3), and 4 seconds (P=0.0002, 95% CI 29.1-32.9) respectively, indicating an increase in functional FVIII activity versus media from the untransfected and null transfected BHK-FVIII cell line. Ligand 7 and ligand 2 both bind to the FVIII light chain, albeit in different regions and with different affinities (data confidential to BAC BV). BAC studies showed that ligand 7 competes with vWF on the FVIII light chain, which is known to increase stability of FVIII in vivo, whereas ligand 2 does not compete for this binding site. The opposing effects of ligand 7 and ligand 2 on FVIII clotting times seen in this study could be due to their differences in FVIII binding properties, since it is known that binding location of FVIII ligands can have an impact on FVIII clotting activity.