Diploma thesis deals with crystalline solar modules i relation to PID phenomenon. The thesis focuses on methods of investigation of changing electrical parameters, in the course of PID degradation, such as prallel resistance and leakage current. The effect of degradation will be examined by V-A characteristics and by electroluminiscence method.

Diploma thesis titled Regeneration of heat and water for paper drying process analyzes use of waste hot air ventilated from drying section of paper mill. Moreover is described detailed design process of recuperation heat exchanger, which should be part of regeneration loop of paper mill. Designed heat exchanger is plate-fin crossflow heat exchanger. For deasign is used – NTU method.

The subject of thesis is an urban revitalization solutions in the territory of Zlín Film Studios, its restoration for film production and other uses. Our vision is to be achieved in this area in the form of interconnected sub-objectives of a compact and fully functional, exceptional unit with the accumulation of education, specific entrepreneurship in the audiovisual industry, a number of civic services, temporary and permanent housing and even facilities for relaxation and sports. The intention reflects the unique history to the maximum possible extent, respects the environment and the existing urban-architectural solutions and creates a new multifunctional public space.

The thesis is focused on material development based on hyaluronic acid usable in regenerative medicine, especially for heart tissue regeneration after myocardial infarction. The object of the study is the oxidized form of hyaluronic acid (HA-Ox) and hydroxyphenyl derivative of HA (HA-TA). HA-Ox can be crosslinked with a bifunctional alkoxyamine POA and HA-TA undergoes an enzymatic reaction in the presence of hydrogen peroxide catalysed by horseradish peroxidase leading to gel formation. To describe the materials, chemical and physical properties, gelation kinetics and conditions of crosslinking reactions were studied. Hydrogels were characterized by mechanical and viscoelastic properties, degradability or stability in simulated body fluids. These hydrogels serve as scaffolds for the selected cell type. To promote cell adhesion and viability, an RGD sequence has been bonded to the structure of HA-TA. This resulting material is also compatible with selected applicators. Its viscosity and extrusion force are low enough to allow application with a catheter with a very small internal diameter. The applicability of the material through the supply tube to the hydrogel reservoir of the second SPREADS device showed good homogeneity, cell distribution and viability. Finally, the material was applied in vivo using these devices during a preclinical study.

Master thesis deals with heat recovery in ventilation systems. Thes is an experimental measurement of the plate heat exchanger for a period of one year. Thes is Compaq es the efficiency of the heat exchanger manufacturer with achal field measurements. The first part describes the theoretical heat recovery systems, thein advantages and disadvantages, the basic formula for calculating efficiency. In the second part I come to the experimental measurements of the plate heat exchanger. There sult of this thesis is to Compaq the efficacy, showing behavior exchanger seasons and energy evaluation.

Master’s thesis deals with design and calculation of one body condensing steam turbine of 25 MW with samples for paper-making industry. The thesis is focused on calculation of the turbine’s heat balance with two technological samples and regeneration which consists of two low pressure heaters and a deaerator with a power reservoir. The steam turbine is designed with a water cooling condenser with down output. The flow part is calculated with lengths of blades. There are basic strength and proportion calculations. In the end of the thesis the turbine’s heat balance is recalculated to 80% and 105% scheme according to an assignment. Another part of this thesis is a drawing of a longitudinal cut of the turbine including a connector with a gearbox.

Mesenchymal stem cells and their regenerative and immunomodulatory potential Abstract Mesenchymal stem cells (MSCs) possess multidirectional regenerative ability, which, together with their immunomodulatory potential, makes them promising cell type for therapy of wide variety of diseases. Despite ongoing research, which proved MSCs application to be safe, reported effect of MSCs administration on patients is not convincingly beneficial yet. In our work we focused on elucidation of MSCs role in regeneration of vital organs, heart and liver, where a large damage is life threatening for patients and any improvement in therapy would save many lives. Similar situation is in Graft versus host disease (GVHD), where MSCs immunomodulatory properties could be beneficial. Role of MSCs in heart regeneration was examined in vitro. Primary adult swine cardiomyocytes (CMCs) were co-cultured with or without swine MSCs for 3 days and morphological and functional parameters (contractions, current, respiration) of CMCs were measured. MSCs showed supportive effect on CMCs survival, especially at day 3 of the experiment, where in co-culture was significantly higher number of viable CMCs with physiological morphology and maintained function. Effect of MSCs on liver regeneration was observed in swine model of chronic liver...

Detailed knowledge of the role that particular genes and factors play during the development and in the normal function of the auditory system is necessary to develop successful regenerative inner ear therapies. Islet1 transcription factor and brain derived neurothrophic factor (BDNF) have great potential to play a role in regenerative inner ear therapy as both have been shown to be sufficient for self-repair regeneration in cochlea in animal studies. In this study we looked at the roles these two factors play in the development and function of the auditory system. In the transgenic mice used in the study, overexpression of Isl1 affected cell specification during embryonic development, leading to enlargement of the cochleovestibular ganglion and accelerated nerve fiber extension and branching in mutant embryos. The hearing of young transgenic mice was not affected. However, it started to decline in 1-month-old animals. This early onset of age-related hearing loss was found to be a consequence of the neurodegeneration of the olivocochlear system caused by Pax2-driven Isl1 misexpression in the hindbrain. Our data provide the first evidence that the alternation of the olivocochlear system efferent system accelerates the age-related functional decline of hearing without the loss of OHCs. The functional role of...

The topic of this diploma thesis is the landscape, specifically the urban landscape of Prague's Palmovka and its various dimensions. In a social anthropological and culturally geographical discourse, the landscape is a complex, multidimensional and multi-layered concept that opens up interesting research questions and imaginations. In this discourse, the landscape is not only a summary of the material aspects of the natural environment around us, but it is also a field of political negotiations, power tensions, experiences, representations and generally interactions between itself and man. From this point of view, the author explores the landscape of Palmovka, which is itself a multi-layered and complex in a view of the diversity of urban structures, historical memos, unrealized visions of development and long-term search for unified concept by various actors during political negotiations that would give this inner city landscape a face. This negotiation is also related to observable processes changing both the physical and social dimension of Palmovka based on efforts to regenerate the area. These processes also include the gentrification which means the migration of young and mostly university-educated people to the older parts of the inner city. Therefore, mainly on the basis of field...

1 Abstract Liver fibrosis is a condition described by extensive accumulation of scar tissue in the liver. With further progression, it leads to cirrhosis or even to hepatocellular carcinoma. Liver fibrosis accompanies every chronic liver disease and its prevalence in adult European population is estimated to be around 4%. During my dissertation work, I studied the function of three members of Metzincin family of metalloproteinases - ADAM17, ADAM10 and MMP-19, in liver fibrosis and liver regeneration using mouse genetic models. ADAM17 and ADAM10 are important regulators of signalling pathways which are involved in immune response as well as differentiation. Both proteases are able to cleave ectodomains of their substrates from cell membrane, affecting bioavailability of ligands and functionality of receptors. Several of their substrates are involved in liver pathologies. MMP-19 on the other hand, is a metalloprotease mainly involved in extracellular matrix cleavage, important process in fibrosis development, as well as resolution of fibrosis. Our results demonstrate that ablation of ADAM10 results in increased susceptibility to liver fibrosis in mice, both spontaneous and toxin induced. ADAM10 deficiency affected biliary epithelium, as we detected higher markers of biliary damage in serum of ADAM10 deficient...