101 |
Transcriptional Profiling of Chromera velia Under Diverse Environmental ConditionsTayyrov, Annageldi 05 1900 (has links)
Since
its
description
in
2008,
Chromera
velia
has
drawn
profound
interest
as
the
closest
free--living
photosynthetic
relative
of
apicomplexan
parasites
that
are
significant
pathogens,
causing
enormous
health
and
economic
problems.
There--
fore,
this
newly
described
species
holds
a
great
potential
to
understand
evolu--
tionary
basis
of
how
photosynthetic
algae
evolved
into
the
fully
pathogenic
Apicomplexa
and
how
their
common
ancestors
may
have
lived
before
they
evolved
into
obligate
parasites.
Hence,
the
aim
of
this
work
is
to
understand
how
C.
velia
function
and
respond
to
different
environmental
conditions.
This
study
aims
to
reveal
how
C.
velia
is
able
to
respond
to
environmental
perturbations
that
are
applied
individually
and
simultaneously
since,
studying
stress
factors
in
separation
fails
to
elucidate
complex
responses
to
multi
stress
factors
and
un--
derstanding
the
systemic
regulation
of
involved
genes.
To
extract
biologically
significant
information
and
to
identify
genes
involved
in
various
physiological
processes
under
variety
of
environmental
conditions
(i.e.
a
combination
of
vary--
ing
temperatures,
iron
availability,
and
salinity
in
the
growth
medium)
we
pre--
pared
strand
specific
RNA--seq
libraries
for
83
samples
in
diverse
environmental
conditions.
Here,
we
report
the
set
of
significantly
differentially
expressed
genes
as
a
re--
sponse
to
the
each
condition
and
their
combinations.
Several
interesting
up--
regulated
and
down--regulated
genes
were
found
and
their
functions
and
in--
volved
pathways
were
studied.
We
showed
that
the
profound
regulation
of
HSP20
proteins
is
significant
under
stress
conditions
and
hypothesized
that
the--
se
proteins
might
be
involved
in
their
movements.
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102 |
Severe Hypoxia Alters Metabolism in Daphnia by Inducing GluconeogenesisMalek, Morad, Yampolsky, Lev C 06 April 2022 (has links)
Hypoxia has become a subject of interest among the many environmental stressors as its role in biology is complex and diverse. Hypoxia is a significant low oxygen condition that causes many pathologies and adaptive responses in organisms. It can lead to a moderate or dangerous loss of respiration and can be an indication of tumorigenesis as many tumors lack adequate blood supply. Organisms possess adaptive responses to hypoxia that include hypoxia-inducible factors (HIFs) that activate several downstream pathways that are responsible for altering metabolism and maintaining homeostasis. Within aquatic organisms, hypoxia is an important ecological constraint as oxygen availability within bodies of water can vary greatly over time and space. Therefore, adaptation to hypoxia is likely pervasive, especially in genotypes originating from bodies of water that are prone to hypoxia. Here we report the transcriptional response to acute hypoxia in the clonal freshwater crustacean Daphnia magna. Daphnia were subjected to 1mg/O2 for 12 hours. Then, RNA was extracted, reverse transcribed, and sequenced using Oxford Nanopore MinION. We find that severe hypoxia significantly up-regulates key enzymes in the gluconeogenesis pathway. Additionally, we report genotype-by-environment interactions showing that Daphnia clones from habitats that are hypoxia prone survive better in hypoxia.
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103 |
The effects of photosymbiosis on gene expression in the facultatively symbiotic coral Astrangia poculata, with a focus on NF-kappaB signaling and antioxidant enzymesNguyen, Linda 09 November 2020 (has links)
Corals are critical to marine biodiversity and human welfare. Coral reefs cover <1% of the seafloor but support ~1/3 of all marine species. Approximately 1.5 billion people live within 100 km of coral reefs, relying upon them for food, income from tourism, and protection from storms. Their economic value has been estimated at $375 billion annually.
The foundation of coral reefs is the intracellular symbiosis between corals and photosynthetic dinoflagellates of the family Symbiodiniaceae. Tropical corals satisfy up to 95% of their nutritional requirements through photosynthesis, and their ability to construct reefs is biochemically coupled to photosynthesis.
While permitting corals to thrive, photosymbiosis also increases their exposure to environmental stressors and vulnerability to climate change. Reliance on photosynthesis restricts reef-building corals to shallow, clear, tropical waters, where they experience higher temperatures and UV exposure. The generation of reactive oxygen species by the symbiont also exposes corals to greater oxidative stress. The symbiosis is particularly sensitive to climate change: all of the mass coral bleaching events have occurred since 1982, driven by elevated ocean temperatures.
Molecular cross-talk between host and symbiont impacts resilience of the coral holobiont and resistance to bleaching. Unfortunately, we know little about how photosymbiosis impacts expression or activity of coral genes. Tropical corals engage in an obligate symbiosis with Symbiodiniaceae, so we cannot study their gene expression in a stable aposymbiotic state. However, the northern star coral, Astrangia poculata, engages in a facultative symbiosis with Symbiodiniaceae.
I used RNA sequencing to investigate how symbiosis impacts gene expression in A. poculata, focusing on genes implicated in photosymbiosis: antioxidant enzymes (specifically superoxide dismutases) and the NF-κB signaling pathway. From an improved transcriptome assembly, I recovered core elements of a primitively simple NF-κB signaling pathway and a rich complement of SOD proteins. 273 coral transcripts—many associated with protein metabolism and vesicle-mediated transport— were differentially expressed in symbiotic versus aposymbiotic corals. Unlike in the facultatively symbiotic sea anemone Exaiptasia, symbiosis was not associated with depressed NF-κB transcript levels. IKKε, a potential positive regulator of NF-κB activity, was strongly up-regulated, as was one particular superoxide dismutase.
|
104 |
Analysis on virus-virus and virus-host interactions in Brassicaceae in natural environments / 野生アブラナ科植物におけるウイルス種間、ウイルス-宿主間相互作用の解析Kamitani, Mari 23 March 2017 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(理学) / 甲第20211号 / 理博第4296号 / 新制||理||1617(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)教授 工藤 洋, 教授 髙林 純示, 教授 田村 実 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM
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105 |
Reference Maps for Comparative Analysis of RNA by LC-MS and RNA SequencingPaulines, Mellie June January 2018 (has links)
No description available.
|
106 |
Influences of Gravitational Intensity on the Transcriptional Landscape of ArabidopsisthalianaMeyers, Alexander D. 02 June 2020 (has links)
No description available.
|
107 |
Integrated Assembly and Annotation of Fathead Minnow Genome Towards Prediction of Environmentarl ExposuresMartinson, John W. 16 June 2020 (has links)
No description available.
|
108 |
Connectivity Analysis of Single-cell RNA-seq Derived Transcriptional SignaturesMahi, Naim January 2020 (has links)
No description available.
|
109 |
Data Driven Approaches for Dissecting Tumor HeterogeneityDurmaz, Arda 27 January 2023 (has links)
No description available.
|
110 |
CHARACTERIZATION OF TCL1-MURINE B-1A CELL TRANSCRIPTOME DYNAMICS REVEALS NOVEL INSIGHTS INTO CHRONIC LYMPHOCYTIC LEUKEMIA ONSETDai, Yuntao 08 October 2015 (has links)
No description available.
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