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O plasma rico em plaquetas (PRP) tem sido utilizado na ortopedia como método para melhorar a cicatrização tecidual. Existem poucos estudos com alto nível de evidência sobre o seu efeito no reparo do manguito rotador e os resultados são conflitantes, não havendo consenso sobre sua eficácia. O objetivo primário deste estudo foi avaliar o efeito do uso do PRP em pacientes submetidos ao reparo do manguito rotador por via artroscópica através da escala da University of California at Los Angeles (UCLA). A avaliação clínica através da escala de Constant-Murley, de dor através da escala visual analógica (EVA), a presença de rerroturas na análise da ressonância magnética (RM) e a ocorrência de complicações foram considerados desfechos secundários. Estudo prospectivo, randomizado, duplo-cego. Dois grupos com 27 pacientes (Grupo PRP e Grupo Controle) foram submetidos ao reparo artroscópico em fileira simples de âncoras, sendo que, no Grupo PRP, foi aplicado o concentrado de plaquetas ao final do procedimento. O PRP foi obtido por aférese, aplicado na consistência líquida, com adição de trombina autóloga. Foram incluídas apenas roturas de espessura completa do supraespinal com retração inferior a 30 mm. Os procedimentos foram realizados pelo mesmo cirurgião, entre setembro de 2008 e abril de 2012. Os desfechos foram avaliados através das escalas da UCLA, de Constant-Murley, EVA e pela RM, pré-operatoriamente e aos 3, 6 e 12 meses. A EVA foi aplicada adicionalmente no primeiro e sétimo dia. O nível de significância empregado foi de 5%. Os pacientes apresentaram melhora clínica significativa com o procedimento nos dois grupos (p < 0,001). Evoluíram de 13,63 ± 3,639 para 30,04 ± 4,528 no Grupo Controle e de 13,93 ± 4,649 para 32,30 ± 3,506 no Grupo PRP aos 12 meses (p = 0,046) de acordo com a escala da UCLA, com um poder de 84% e tamanho do efeito de 0,56. De acordo com a escala de Constant-Murley, os pacientes evoluíram de 47,37 ± 11,088 para 76,89 ± 13,198 no Grupo Controle e de 46,96 ± 11,937 para 83,26 ± 11,141 no Grupo PRP aos 12 meses (p=0,061). A avaliação aos 3 e 6 meses não demonstrou diferença significativa. A avaliação pela EVA não demonstrou diferença estatística em nenhum dos tempos de seguimento, evoluindo de 7,00 ± 1,939 no Grupo Controle e de 6,67 ± 1,617 no Grupo PRP no pré-operatório para 1,70 ± 2,127 e 1,04 ± 1,808, respectivamente, aos 12 meses (p = 0,220). Na análise pela RM, o Grupo Controle apresentou uma rerrotura completa e quatro parciais, enquanto o Grupo PRP apresentou duas rerroturas parciais (p = 0,42). Ocorreu um caso de rigidez articular em cada grupo (p = 1). O PRP obtido por aférese, aplicado na consistência líquida e com adição de trombina propiciou melhores resultados pela escala da UCLA aos 12 meses de pós-operatório / Platelet-rich plasma (PRP) has been used in orthopedics as a method to enhance tissue healing. There are few studies with a high level of evidence about its effect on rotator cuff repair, and the results are conflicting, with no consensus about its effectiveness. Clinical assessment as measured by the UCLA (University of California at Los Angeles) shoulder rating scale was established as the primary outcome. The secondary outcomes included clinical assessment on the Constant-Murley scale, pain as measured by a visual analog scale (VAS), the retear rate assessed by magnetic resonance imaging (MRI) and the complication rate. A prospective, randomized, doubleblind trial was conducted. Two groups of 27 patients (PRP Group and Control Group) were subjected to arthroscopic single-row repair. Liquid PRP prepared by apheresis was applied to the PRP Group at the end of the surgical procedure, with autologous thrombin. Complete supraspinatus tears with retraction less than 30 mm were included. The procedures were performed by the same surgeon between September 2008 and April 2012. Outcomes were assessed using UCLA and Constant-Murley scales, VAS and magnetic resonance imaging preoperatively and at 3, 6 and 12 months. The VAS was also applied on days one and seven. The significance level was 5%. The two groups of patients exhibited significant clinical improvement (p < 0.001). The score on the UCLA scale increased from 13.63 ± 3.639 to 30.04 ± 4.528 and from 13.93 ± 4.649 to 32.30 ± 3.506 in the Control and PRP groups, respectively, between the preoperative assessment and after 12 months (p=0.046), with a 84% power and a 0.56 effect size. The score on Constant-Murley scale increased from 47.37 ± 11.088 to 76.89 ± 13.198 in the Control Group and from 46.96 ± 11.937 to 83.26 ± 11.141 in the PRP Group (p = 0.061). Assessment at 3 and 6 months did not identify significant differences. The VAS did not statistically differ at any investigated timepoint and varied from 7.00 ± 1.939 and 6.67 ± 1.617 before surgery to 1.70 ± 2.127 and 1.04 ± 1.808 at the 12-month assessment in the Control and PRP groups, respectively (p = 0.220). The Control Group exhibited one case of complete and four of partial retears, and the Group PRP exhibited two cases of partial retears (p = 0.42). PRP prepared by apheresis, applied in the liquid state with thrombin, promoted better results on the UCLA scale 12 months after surgery

The thesis describes methods for spectrum monitoring, which are used by the Czech telecommunication office. The thesis describes a design of an RF unit for a portable monitoring station and its potential use. The RF unit consists of several devices, which are connected. Devices like an antenna, a rotator, a rotator control unit, coaxial switches, an amplifier and attenuator were chosen as commercially available devices. It is also described, why were these devices chosen. Other devices such as filters, a control unit for the RF unit and a power supply were designed. The RF unit is controlled by a computer. A software for spectrum monitoring contains a graphical user interface for the RF unit control.

The evaluation of shoulder abduction and flexion strength is important in the rehabilitation after rotator cuff tear. The purpose of this work is to assess the intra and inter-rater reliability of measurement data from an isokinetic dynamometer used to evaluate shoulder abduction and flexion strength, with the aim to identify sources of measurement errors and suggest improvements. The measurement data was collected by a research group at Karolinska Institute and contained load and torque data from thirteen healthy subjects in the ages of 25 to 87 years. The measurements were carried out on two occasions, one week apart. Systematic differences between occasions are analyzed using the Shapiro Wilk test, the paired t-test, and Wilcoxon signed rank test. The agreement of the measurements is analyzed quantitatively using the coefficient of variation and the Bland Altman plot, and quantitively, using the intraclass correlation coefficient. A significant systematic difference in shoulder abduction and flexion load measurements was found, and the recommendation to prevent this is that components should be calibrated in a standardized way. The measurements showed varying reliability within and between measurement occasions and that after familiarization with the isokinetic dynamometer, repeatability improved. The findings indicate a need of a standardized protocol for patient education and placement. Measurements from the position sensor contributed to more random torque values. To improve the repeatability in measurements from the position sensor, axis of rotation should be kept aligned. An increasing variability in measurements with increasing load and torque was found. The recommendation is to use a preload for patients using more force in the movement, to make sure a preset speed is not exceeded, which contributes to more reliable measurements. / Utvärderingen av axelabduktion och flexionsstyrka är viktig i rehabiliteringen efter skada i axelleden. Syftet med det här arbetet är att bedöma intra- och interbedömartillförlitligheten hos mätdata från en isokinetisk dynamometer som används för att utvärdera axelabduktion och flexionsstyrka, med syftet att identifiera källor till mätfel och föreslå förbättringar. Mätdatat samlades in av en forskargrupp vid Karolinska Institutet och innehöll belastnings- och vridmomentdata från tretton friska försökspersoner i åldrarna 25 till 87 år. Mätningarna utfördes vid två tillfällen med en veckas mellanrum. Systematiska skillnader mellan tillfällena analyseras med Shapiro Wilk-testet, det parade t-testet och Wilcoxon signed rank test. Mätningarnas överensstämmelse analyseras kvantitativt med hjälp av variationskoefficienten och Bland Altman-diagrammet, samt kvalitativt med hjälp av intraklasskorrelationskoefficienten. En signifikant systematisk skillnad i axelabduktion och flexionsbelastningsmätningar hittades, och rekommendationen för att förhindra detta är att komponenter bör kalibreras på ett standardiserat sätt. Mätningarna visade på en varierande tillförlitlighet inom och mellan mättillfällen och att efter bekantskap med den isokinetiska dynamometern, förbättrades repeterbarheten. Slutsatserna indikerar ett behov av ett standardiserat protokoll för patientutbildning och placering. Mätningar från positionssensorn bidrog till mer slumpmässiga vridmomentvärden. För att förbättra repeterbarheten i mätningar från positionssensorn bör rotationsaxeln hållas i linje. En ökande variation mellan mättillfällen med ökande belastning och vridmoment hittades. Rekommendationen är att använda en förspänning för patienter som använder mer kraft i rörelsen, för att säkerställa att en förinställd hastighet inte överskrids, vilket bidrar till mer tillförlitliga mätningar.

La douleur, fréquemment rencontrée lors d’atteintes musculosquelettiques, est un phénomène complexe influencé par une combinaison multifactorielle de déterminants biopsychosociaux. Elle présente un important taux de chronicité avec près de huit millions de personnes au Canada atteintes de douleur chronique (douleur qui perdure plus de trois mois), c’est-à-dire un Canadien sur cinq. Les troubles musculosquelettiques incluent le syndrome de conflit sous-acromial (SCSA), une atteinte fréquente à l’épaule, qui a une forte tendance à se chroniciser. Un an après le début des symptômes, environ la moitié des personnes avec ce syndrome présentent de la douleur et une limitation de la fonction persistantes. Des facteurs psychosociaux seraient prédictifs de la chronicité pour le SCSA. Toutefois, dans les guides de pratique qui orientent la prise en charge de cette atteinte, les interventions ciblant ces facteurs sont limitées et parfois même manquantes. En physiothérapie, une discipline fréquemment impliquée dans la réadaptation de cette atteinte, des approches englobant les facteurs biopsychosociaux seraient à privilégier. L’enseignement au patient, une modalité faisant partie intégrante de la physiothérapie, mais pour laquelle les recommandations sont limitées pour en guider la réalisation, serait une avenue pertinente. Cette thèse vise à développer des recommandations qui soutiennent l'enseignement structuré auprès des patients ciblant l’autonomisation (implication active pour la prise de décision et la gestion de la condition) de la personne dans le suivi du SCSA en physiothérapie. Elles intègrent les données scientifiques, expérientielles et contextuelles et les composantes de la Classification internationale du fonctionnement, du handicap et de la santé. La première phase de cette thèse contient une revue systématique de la portée qui visait à répertorier l’étendue de la littérature sur les interventions d’enseignement au patient lors de la prise charge du SCSA. Cette revue a permis d’identifier des cibles initiales d’enseignement au patient issues de la littérature, utilisées pour orienter la phase suivante du projet. La deuxième phase a utilisé une consultation d’experts par des groupes de discussion focalisée intégrant des professionnels de la réadaptation et des patients-partenaires pour explorer les cibles et décrire les stratégies d’enseignement (plan pour atteindre un objectif d’enseignement visé). Cette consultation a été analysée de manière qualitative en vue de faire ressortir les thématiques principales d’enseignement au patient émergeant des discussions. Cette étape a mené au développement de recommandations préliminaires. La troisième phase visait à obtenir un consensus sur la pertinence et la formulation de recommandations détaillées encadrant les interventions d’enseignement au patient en physiothérapie pour le SCSA à l’aide d’une consultation par approche Delphi. À la suite de ces trois phases, sept recommandations générales encadrant les stratégies d’enseignement au patient et six recommandations touchant des cibles d’interventions spécifiques d’enseignement ont été développées. Un outil d’aide à la décision guidant l’utilisation de ces recommandations d’enseignement structuré lors du suivi des personnes atteintes d’un SCSA a aussi été élaboré. Ces recommandations sont les premières existantes pouvant soutenir l’enseignement structuré au patient lors du suivi du SCSA. Celles-ci sont pertinentes pour guider l’enseignement prodigué basé sur la littérature et un consensus d’experts. Les cibles d’autogestion et d’engagement actif de la personne sont pertinentes pour intervenir sur les facteurs psychosociaux et ainsi potentiellement réduire la chronicité de cette atteinte. / Pain, frequently encountered in musculoskeletal injuries, is a complex phenomenon influenced by a multifactorial combination of biopsychosocial determinants. It has a high rate of chronicity with nearly eight million people in Canada suffering from chronic pain (pain lasting more than three months), i.e., one in five Canadians. Among musculoskeletal disorders, subacromial pain syndrome (SAPS), a frequently encoutered shoulder disorder, has a high propensity to become chronic. One year after the onset of symptoms, about half of individuals with SAPS have persistent pain and functional limitations. Psychosocial factors are predictive of SAPS chronicity, however, in the clinical practice guidelines that guide the management of this condition, interventions targeting these factors are limited and sometimes absent. Approaches that target biopsychosocial factors should be favoured, among others, in physiotherapy, a discipline frequently involved in the rehabilitation of this condition. Patient education, a modality that is an integral part of physiotherapy, but for which there is limited evidence and recommendations to guide its implementation, would be a relevant avenue. Therefore, this thesis aims to develop recommendations that support structured patient education. These recommendations focus on the person's empowerment and self-management during the follow-up of SAPS in physical therapy. They are based on scientific, experiential and contextual data as well as components of the International Classification of Functioning, Disability and Health. The first phase of this thesis contains a systematic scoping review that aimed to map the extent of the literature on patient education interventions in the management of SAPS. This review identified the initial patient education targets from the literature that were used to guide the next phase. The second phase used expert consultation through focus groups involving rehabilitation professionals and patient-partners to explore targets and outline strategies for structured patient education interventions in physical therapy for the follow-up of individuals with SAPS. The development of the interview guide and the analysis of these discussions were based on the initial educational targets and strategies (plan to conduct the educational intervention) extracted from the previous phase. This consultation was qualitatively analyzed to identify the main patient education themes resulting from the discussions. The main themes led to the development of preliminary recommendations. The third phase aimed to reach consensus on the relevance and formulation of detailed recommendations framing patient education interventions in physical therapy for the follow-up of individuals with SAPS using a Delphi consultation approach. As a result of these three phases, seven general recommendations framing patient teaching strategies and six recommendations for specific teaching interventions targets were developed. A decision support tool guiding the use of these structured patient education recommendations in the follow-up of people with SAPS has also been developed. These recommendations are the first that can comprehensively support structured patient education intervention during the follow-up of individuals with SAPS. They are relevant to guide patient education interventions that are based on the literature and expert consensus. The goals of self-management and active engagement of the person are relevant to intervene on psychosocial factors and thus potentially reduce the chronicity of this condition.