Antioxidant activity of dried plum powder (DPP) at 3% was compared to rosemary extract (RE) at 0.05% in turkey breakfast sausages which were stored under three aerobic conditions: raw refrigerated (RR; 6 degrees C), raw and precooked frozen (RF and PF, respectively; -23 degrees C). Sausage links were tested on specific days over a 56 day shelf-life. Analyses included pH, proximate composition, objective color (CIE L*, a*,b*), lipid oxidation (TBARS), aerobic plate count and enumeration of lactic acid bacteria, sensory, reheat yields and shear force. All RR treatments surpassed the threshold of 3 for TBARS by day 6. Spoilage occurred by day 3 for all RR treatments (> 6.0 log10). Treatments containing DPP had lower TBARS values for RF (P < 0.05) and PF (P &lt; 0.01). DPP containing treatments had lower external and internal L* values and a* values while internal b* values were higher. Sensory scores for plum and sweet were higher, while cardboard and rancidity were lower in treatments containing DPP. Sensory scores for cardboard and rancidity were lower for RF and higher for PF treatments. Reheated cooked yields and shear force values for PF treatments held either 15 or 30 min showed an increasing trend with subsequent storage. Proximate composition data showed no significant differences for fat, moisture, and protein. DPP containing treatments were different from other treatments having a lower pH except for the PF RE treatment. The addition of DPP at 3% into a turkey breakfast sausage had an inhibitory effect on lipid oxidation in a RF and PF treatments. Inclusion of DPP darkened the external and internal appearance and increased yellowness as well as increasing the sweetness and plum flavor.

The development of a reduced energy Thai style fresh pork sausage has been achieved by replacing fat with carbohydrate and water. Replacing fat in the processed meat formulation with other ingredients is known to alter the product qualities. The addition of carbohydrate, namely mung bean vermicelli, as a fat replacement is suggested to improve the water holding capacity, cooking yield and textural properties as well as reduce the fat and total energy content. This research developed a standard processing methodology for a characteristic Thai fresh pork sausage. The research evaluated the potential of mung bean vermicelli in the formulation of a reduced energy Thai style fresh pork sausage. The reduced energy sausage was developed by replacing fat with 25%, 50%, 75%, and 100% soaked mung bean vermicelli. Quality characteristics of the sausages (chemical, textural and sensory) were evaluated and compared with control sausages (20% fat). The results of the present study indicated that soaked mung bean vermicelli offset many of the characteristic changes usually brought about by fat reduction. The sausages had a decreased cooking loss and an improved yield as well as a reduction in energy content. In addition, they maintained the textural and sensory characteristics of the control Thai style fresh pork sausages. / Master of Science (Hons)

Published Article / An experiment was conducted to investigate the effects of substituting wheat flour (WF) for cassava flour (CF) on chemical composition, storage stability and sensory qualities of beef sausage. The WF in the sausage was replaced with CF at 0, 25, 50, 75 and 100% levels. Values obtained for protein content were comparable with one another. However, fibre content increased with the increase in the levels of CF in the sausage. The highest fibre content of 0.43% was recorded in sausage with 15% CF. Fat content tended to decrease with increase in the levels of CF in the sausage. Values obtained for cooking and refrigeration losses were comparable with one another. Cooking losses ranged between 1.08 - 1.85% while refrigeration loss ranged from 7.60-8.53%. Compared with the control, higher panel scores were recorded on sensory qualities for sausages containing CF. WF in beef sausage can be substituted with 100% CF without adverse effect on chemical composition, processing yield or sensory qualities. This approach will satisfy the growing demand of consumers for gluten-free meat products, thereby minimising the incidence of coeliac disease in humans.

Para-hydroxybenzyl isothiocyanate (p-HBIT) formed in yellow mustard following hydrolysis of the glucosinolate, sinalbin, is a natural antimicrobial agent. p-HBIT is not dependably available commercially, and a small amount was synthesized for use in beaker sausage fermentations. For these trials stabilized p-HBIT was used in dry sausage meat batter to reduce the viability of inoculated Escherichia coli O157:H7. A >4 log CFU/g reduction of E. coli O157:H7 was achieved in the beaker sausage containing p-HBIT. For sensory evaluation of fermented sausages containing ≤ 4% (w/w) yellow mustard powder, consumer preference tests were done. Deodorized (deheated) yellow mustard was added at 1,2,3 and 4% (w/w) to dry-fermented sausage and it was found that 3% and 4% mustard negatively affected the flavour, texture and overall acceptability of the fermented sausage. Dry-fermented sausage containing 1% and 2% mustard had a slight change in flavour, texture and overall acceptability.

Para-hydroxybenzyl isothiocyanate (p-HBIT) formed in yellow mustard following hydrolysis of the glucosinolate, sinalbin, is a natural antimicrobial agent. p-HBIT is not dependably available commercially, and a small amount was synthesized for use in beaker sausage fermentations. For these trials stabilized p-HBIT was used in dry sausage meat batter to reduce the viability of inoculated Escherichia coli O157:H7. A >4 log CFU/g reduction of E. coli O157:H7 was achieved in the beaker sausage containing p-HBIT. For sensory evaluation of fermented sausages containing ≤ 4% (w/w) yellow mustard powder, consumer preference tests were done. Deodorized (deheated) yellow mustard was added at 1,2,3 and 4% (w/w) to dry-fermented sausage and it was found that 3% and 4% mustard negatively affected the flavour, texture and overall acceptability of the fermented sausage. Dry-fermented sausage containing 1% and 2% mustard had a slight change in flavour, texture and overall acceptability.

The objectives of this study were to determine the effects of deboning time (pre- and post-rigor), processing steps (grinding - GB; salting - SB; batter formulation - BB), and storage time on the microbiological, technological, sensory, and willingness-to-pay attributes of beef sausage. Using the chuck primals from 5 24-month-old Holstein steers, beef was deboned within 2 h post-mortem (pre-rigor) or 72 h (post-rigor) and processed to sausages using beef bratwurst spice mix, water/ice slurry, corn syrup, erythorbate, nitrite, salt, and 0.25% w/w sodium tripolyphosphate and a typical cooking cycle ending at 74 ¡ãC. Samples were collected during grinding, salting, batter formulation, and storage of cooked sausages. Beef deboning time did not influence bacterial counts (P ¡Ý 0.138). However, salting of raw ground beef resulted in a 0.4-log reduction in both aerobic plate count and Salmonella (P ¡Ü 0.001). Lactic acid bacteria increased from 0.5 log on d 0 to 3.8 log on d 120 of vacuum storage (P ¡Ü 0.019). The pH was greater in pre-rigor GB and SB than in post-rigor GB and SB (P < 0.001). However, deboning time had no effect on metmyoglobin reducing activity, cooking loss, and color of raw beef mixtures. Protein solubility of pre-rigor SB (124.3 mg/kg) tended to be greater than that of post-rigor SB (113.9 mg/kg; P = 0.071). TBARS were greater in BB but decreased during vacuum storage of cooked sausage (P ¡Ü 0.018). With the exception of chewiness and saltiness being 52.9 N-mm and 0.3 points greater in post-rigor sausage (P = 0.040 and 0.054, respectively), instrumental texture profile analysis and descriptive sensory analysis detected no difference between pre- and post-rigor sausages (P ¡Ý 0.153). Consumers preferred the aroma, flavor, and overall acceptability of pre-rigor sausage when compared to post-rigor sausage (P = 0.008, 0.029, and 0.011, respectively). Average predicted demand was 0.7 lb greater for pre-rigor than post-rigor sausage at any price point from $1/lb to $10/lb (P ¡Ü 0.001).

Sodium lactate and acetic acid derivatives were evaluated for their effects on color retention, microbial growth (TPC), oxidation (TBARS), and sensory attributes of hot-boned pork sausage patties that were stored under retail store display conditions over time. Treatments included: (a) 2.5% sodium lactate 60% solids (L), (b) 2.5% buffered vinegar pH 6.5-8.0 (V), (c) 2.5% sodium lactate and vinegar 52/48% mixture (LV), (d) control with 0.02% BHA/BHT (C), and (e) negative control without additives (NC). Overall acceptability of day 17 LV and L treatments were not different (P>0.05) from day 14 treatments. These results revealed that the L and LV sausage patties retained sensory acceptability and microbial quality from day 14 through day 17 as opposed to other treatments. Additionally, sausage patties with 2.5% LV maintained color (redness) and overall acceptability throughout 17 days of shelf-life when held in retail conditions, when compared to other treatments.

Darbo objektai: Virtų, karštai rūkytų dešrų gamyba, juslinių savybių ir mikrobiologinis dešrų įvertinimas. Darbo tikslas – Įvertinti natrio druskų mažinimo galimybes termiškai apdorotuose mėsos gaminiuose. Darbo užduotys: 1. Mokslinės literatūros apie natrio druskų mažinimo galimybes termiškai apdorotuose mėsos gaminiuose apibendrinimas. 2. Natrio druskų pakeitimo alternatyviais ingredientais virtose ir karštai rūkytose dešrose galimybių tyrimas. 3. Gaminių receptūrų sudarymas, gaminių gamyba, juslinis įvertinimas. 4. Gautų rezultatų statistinis apdorojimas, apibendrinimas, išvadų formulavimas. Darbo rengimo vieta: Tiriamasis darbas buvo atliktas Lietuvos sveikatos mokslų universitete Veterinarijos akademijoje Maisto saugos ir kokybės katedroje ir „X“ mėsos perdirbimo įmonėje. Tyrimai buvo atlikti Šiaulių Nacionalinio maisto ir veterinarijos rizikos vertinimo institute. Darbo apimtis: 46 puslapiai, 11 lentelės, 10 paveikslų, 4 priedai Raktažodžiai: virta dešra, karštai rūkyta dešra, natrio druska Išvados: 1. 50 proc. nitritinės druskos kiekio pakeitimas kalcio laktatu virtose dešrose ženkliai padidino aerobinių mikroorganizmų skaičių, todėl toks pakeitimas nėra tinkamas taikyti virtų dešrų gamyboje. Kiti tyrime tirti natrio nitrito pakaitalai virtose ir karštai rūkytose dešrose nežymiai padidino aerobinių mikroorganizmų skaičių; 2. Nitritinės druskos pakeitimas 50 proc. kalio chloridu ir 50 proc. kalcio laktatu turėjo nežymią įtaką virtų dešrų juslinėms savybėms bei... [toliau žr. visą tekstą] / Objects of the thesis: Production of cooked, smoked sausages, evaluation of sausages of organoleptic characteristics and microbiological. Aim of the thesis is to rate the possibilities of sodium salt reduction in the heat-treated meat products. Purpose of the thesis: 1. Summary of the scientific literature on possibilities of sodium salt reduction in the heat-treated meat products. 2. Research of possibilities of the change of the sodium salt by the alternative ingredients in the cooked and smoked sausages. 3. Formation of the products recipes, manufacture of the products and sensory evaluation. 4. Statistical processing of the obtained results, generalization, and formulation of conclusions. Work preparation place ant term: The research was performed at the Food Safety and Quality Department of the Veterinary Academy of the Lithuanian University of Health Sciences and at the company of meat processing “X”. The research was performed at the Šiauliai National Food and Veterinary Risk Assessment Institute. Work span: The thesis consists of: 46 pages, 11 tables, 10 pictures, 4 annexes Keywords: cooked sausage, smoked sausage, sodium salt Conclusions: 1. 50% nitric salt replacement to calcium lactate leads to increase of aerobic microorganisms in cooked sausages and it is therefore unsuitable for the production of cooked sausages. The other investigated nitric salt replacement variants do not show a significant increase of aerobic microorganisms in cooked and smoked... [to full text]

Master of Science / Department of Animal Sciences & Industry / Elizabeth A. Boyle / Quality attributes of vacuum packaged, skinless smoked sausage made with a combination of pork, turkey, and beef, cooked to 64, 68, or 72°C internal endpoint temperature following USDA FSIS Appendix A, and displayed at 4°C for up to 120 days under light emitting diode (LED) and fluorescent (FLS) lighting were evaluated. External color, pH, thiobarbituric acid reactive substances (TBARS), proximate analysis, reheat yield, and sensory attributes were measured on day 0, 90, and 120 of display. Purge amount and color were measured on day 10, 90, and 120. Product was displayed in LED or FLS retail display cases set to the same operational and temperature profiles. Lighting type had no effect (P>0.05) on any of the measured attributes. Instrumental external color was less (P<0.05) red by 0.63 units in product thermally processed to 64°C than product processed to 68°C. Product cooked to 72°C was less (P<0.05) yellow externally compared to those processed to 64 and 68°C. Purge color lightness increased (P<0.05) in product thermally processed to 72 compared to 64°C. Purge was more red by 0.36 units (P<0.05) on day 120 compared to day 10. Yellowness of purge color increased at 72°C compared to 64°C by 0.66 units. Purge was more yellow (P<0.05) on d 120 compared to d 10 and 90. TBARS values decreased (P<0.05) from 0.70 mg of malonaldehyde/100g on day 0 to 0.35 and 0.23 on d 90 and 120, respectively. Sensory panel scores showed that flavor intensity decreased (P<0.05) as day of storage increased, and saltiness decreased from d 0 to d 90. Purge content increased (P<0.05) from 1.45% to 1.90% in products cooked to 64 and 68°C, respectively. The amount of purge increased (P<0.05) from 1.58% to 1.92% on day 10 and 90, respectively. While there were slight changes found in quality characteristics of smoked sausages during storage, many of these were minimal. Processors could reduce their internal endpoint temperature following USDA FSIS Appendix A guidelines with minimal effect on product quality. Vacuum packaged pre-cooked smoked sausages could be displayed under LED or FLS lighting with no effect on product quality.

Reações de oxidação lipídica são responsáveis pelo desenvolvimento de sabores e odores desagradáveis tornando os alimentos impróprios para consumo, sendo necessário o uso de antioxidantes. O ácido ascórbico (AA) é um antioxidante muito eficaz, que exibe função vitamínica, no entanto é relativamente instável. Com o objetivo de aumentar a estabilidade do AA e, consequentemente, facilitar sua aplicação em produtos alimentícios, os métodos de encapsulação por coacervação complexa e por dispositivos microfluídicos foram testados. Primeiramente foi apresentada a Revisão Bibliográfica no Capítulo 1, e em seguida, a encapsulação por coacervação complexa, como será visto no Capítulo 2. Neste caso, nove tratamentos foram obtidos utilizando-se gelatina e goma arábica como materiais de parede e analisados com relação à morfologia, por microscopia ótica e eletrônica de varredura, umidade, atividade de água, higroscopicidade, solubilidade, potencial zeta, espectroscopia no infravermelho por transformada de Fourier (Ftir), tamanho e distribuição de tamanho de partículas, cor instrumental, eficiência de encapsulação e estabilidade do material encapsulado. No capítulo 3 serão apresentados resultados obtidos na encapsulação do AA pelo método de dispositivos microfluídicos. Cinco tratamentos foram obtidos, sendo analisados com relação à morfologia, por microscopia ótica, eletrônica de varredura e confocal, eficiência de encapsulação, tamanho e distribuição de tamanho de partícula e estabilidade do material encapsulado. A obtenção das microcápsulas de AA pelos dois métodos citados foi viável uma vez que apresentaram altos valores de eficiência de encapsulação e ótima atuação em relação à proteção do AA durante estocagem. Comparando-se os dois métodos, as cápsulas obtidas por dispositivos microfluídicos conferiram melhor estabilidade ao ácido ascórbico, no entanto amostras obtidas por coacervação complexa foram aplicadas em salsicha devido a maior quantidade de AA presente em sua constituição. O efeito da aplicação das microcápsulas nas salsichas foi avaliado durante 40 dias de armazenamento refrigerado como será visto no Capítulo 4. Cinco tratamentos foram elaborados e analisados de acordo com a estabilidade da emulsão cárnea durante o processamento, umidade, atividade de água, alteração do pH, determinação da cor instrumental, perfil de textura instrumental, estabilidade oxidativa pelo método de substâncias reativas ao ácido tiobarbitúrico (TBARS) e aceitação sensorial. A aplicação das microcápsulas de AA em salsicha foi possível sem comprometer a qualidade do produto final. Todos os dados obtidos foram analisados estatisticamente por análise de variância ANOVA e teste de Tukey, ao nível de 5% de significância com auxílio do programa SAS. Os experimentos relacionados à encapsulação por coacervação complexa e aplicação das microcápsulas em salsicha foram realizados no Laboratório de Produtos Funcionais, nas dependências do Departamento de Engenharia de Alimentos da FZEA/USP. Os experimentos relacionados à utilização de dispositivos microfluídicos foram realizados nos laboratórios do professor David A. Weitz, da Escola de Engenharia e Ciências Aplicadas de Harvard, da Universidade de Harvard, Cambridge, Estados Unidos. / Lipid oxidation reactions are responsible for the development of unpleasant tastes and odors making food unfit for consumption. For this reason, the use of antioxidant is necessary. Ascorbic acid (AA) is a very effective antioxidant with vitamin function, however it is relatively unstable. With the aim of increasing the stability of AA and thus improve its application in food products, the methods of encapsulation by complex coacervation and microfluidic devices were tested. First of all the Literature Review is presented in Chapter 1. The encapsulation by complex coacervation can be seen in Chapter 2. For this methodology, nine treatments were obtained using gelatin and gum Arabic as encapsulant agent and analyzed regarding to morphology by optical and scanning electron microscopy, moisture, water activity, hygroscopicity, solubility, Zeta Potential, Fourier transform infrared Spectroscopy (FTIR), particle size and particle size distribution, instrumental color, encapsulation efficiency and stability of the encapsulated material. The results obtained for AA encapsulation by microfluidic device will be presented in Chapter 3. Five treatments were obtained and analyzed regarding to morphology by optical, scanning electron and confocal microscopy, encapsulation efficiency, particle size and particle size distribution and stability of the encapsulated material. The production of AA microcapsules by the two methods mentioned was feasible once that showed high levels of encapsulation efficiency and optimal performance regarding to the protection of AA during storage. Comparing the two methods, the microcapsules obtained by microfluidic device conferred better stability to AA, however samples obtained by complex coacervation were applied in sausage due to the greater amount of AA in its constitution. The effect of the application of microcapsules in sausages was evaluated during 40 days at refrigerated storage as it will be seen in Chapter 4. Five treatments were prepared and analyzed according to the stability of the meat emulsion during processing, moisture, water activity, pH changes, determination of instrumental color, instrumental texture profile, oxidative stability by the method of thiobarbituric acid reactive substances (TBARS) and sensory acceptance. The application of AA microcapsules in sausage was possible without compromising the quality of the final product. All data were statistically analyzed by ANOVA and Tukey test, at 5% of significance with the use of SAS software. The experiments related to encapsulation by complex coacervation and application of microcapsules in sausage were carried out at Laboratory of Functional Products, at Department of Food Engineering, FZEA / USP. The experiments related to the use of microfluidic devices were performed in the laboratories of Professor David A. Weitz, at School of Engineering and Applied Sciences of Harvard, at Harvard University, Cambridge, USA.