Submitted by Gabriela Lopes (gmachadolopesufpel@gmail.com) on 2016-10-13T14:17:26Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese CARI REJANE FISS TIMM.pdf: 1890245 bytes, checksum: 3dd97e2c8ea44f66e16d8dc12f151fc0 (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-01-04T14:54:11Z (GMT) No. of bitstreams: 2 Tese CARI REJANE FISS TIMM.pdf: 1890245 bytes, checksum: 3dd97e2c8ea44f66e16d8dc12f151fc0 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-01-04T14:54:11Z (GMT). No. of bitstreams: 2 Tese CARI REJANE FISS TIMM.pdf: 1890245 bytes, checksum: 3dd97e2c8ea44f66e16d8dc12f151fc0 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-08-31 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / Existem muitas informações referentes à cultura do pessegueiro. Entretanto, como o dinamismo da pesquisa, da fruticultura e do mercado consumidor causa mudanças, estas irão exigir um sistema moderno e tecnificado de produção de mudas de qualidade genética e sanitária para obtenção de frutos de qualidade e uma alta produtividade. Com o objetivo de adequar uma metodologia de propagação para a produção de mudas de qualidade, foram desenvolvidos vários experimentos com cultivares copa e porta-enxertos de Prunus spp. para avaliar os efeitos de diferentes concentrações de ácido indolbutírico e do tipo de miniestaca no enraizamento, determinar o tempo ótimo de enraizamento de miniestacas e identificar nas fases de estabelecimento e multiplicação o meio de cultura e a concentração de BAP adequados, na propagação in vitro para obtenção de mudas. Os experimentos foram realizados em casa de vegetação e no Laboratório de Micropropagação de Plantas Frutíferas do Departamento de Fitotecnia da Faculdade de Agronomia Eliseu Maciel da Universidade Federal de Pelotas, no período de março de 2014 a fevereiro de 2016. Para os experimentos de enraizamento foram utilizados os porta-enxertos das cultivares Nemaguard, Flordaguard, Okinawa, umezeiro, Mr.S 2/5 e as cultivares copa Capdeboscq, Bonão e Maciel, testando-se os tipos de miniestacas, as diferentes concentrações de AIB e determinando o tempo ótimo de enraizamento das miniestacas. No estabelecimento e na multiplicação foram utilizados os portaenxertos umezeiro e Marianna para determinar o meio de cultura e a concentração de BAP. No enraizamento, as miniestacas apicais, medianas e basais resultaram com a maior porcentagem utilizando-se 1.000 mg L-1 de AIB e determinando o tempo ótimo de enraizamento Flordaguard obteve 70% aos 50 dias de cultivo. Na fase de estabelecimento observou-se que o meio de cultivo WPM acrescido de 1.0 mg L-1 de BAP demonstrou ser o mais adequado. Na multiplicação, verificou-se que a cultivar Marianna com 1.5 mg L-1 de BAP foram superiores para altura da brotação, número de gemas, número e comprimento das brotações massa fresca e taxa de multiplicação. / There are many information regarding the peach crop. However, as the dynamism of research, horticulture and consumer market causes changes, these will require a modern and technified production of genetic and health quality system to obtain seedlings of fruit quality and high productivity. In order to adapt a method of propagation for the production of quality seedlings, they were developed several experiments with cultivars crown and rootstock Prunus spp. to evaluate the effects of different indolebutyric acid concentration and type of minicuttings rooting, determining the optimum time of cuttings rooting and identify the establishment and multiplication stage culture medium and concentration of appropriate BAP in vitro propagation in obtaining seedlings. The experiments were conducted in a greenhouse and Fruit trees Plant Micropropagation Laboratory of the Department of Plant Science, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, from March 2014 to February 2016. For rooting experiments were used rootstocks of Nemaguard cultivars Flordaguard, Okinawa, mume, Mr.S 2/5 and cultivars Capdeboscq, Bonao and Maciel, testing themselves the types of cuttings, different concentrations of IBA and determining the optimum time for rooting of cuttings. In the establishment and multiplication were used the rootstocks mume and Marianna to determine the culture medium and the concentration of BAP. In the rooting cuttings apical, middle and basal resulted with the highest percentage using 1,000 mg L-1 IBA and determining the rooting great time Flordaguard obtained 70% after 50 days of cultivation. In the establishment phase it was observed that the WPM culture medium supplemented with 1.0 mg L-1 BAP proved to be the most suitable. In multiplying it was found that cultivating Marianna 1.5 mg L-1 BAP were superior to the height of the time of budding e, number and length of shoot fresh weight and multiplication rate.

Please read the abstract in the dissertation. / Dissertation (MSc)--University of Pretoria, 2009. / Chemical Engineering / unrestricted

Individual differences in fecundity often serve as proxies for differences in overall fitness, especially when it is difficult to track the fate of an individual's offspring to reproductive maturity. Using fecundity may be biased, however, if density-dependent interactions between siblings affect survival and reproduction of offspring from high- and low-fecundity parents differently. To test for such density-dependent effects in plants, we sowed seeds of the wildflower Ipomopsis aggregata (scarlet gilia) to mimic partially overlapping seed shadows of pairs of plants, one of which produced twice as many seeds. We tested for differences in offspring success using a genetic marker to track offspring to flowering multiple years later. Without density dependence, the high-fecundity parent should produce twice as many surviving offspring. We also developed a model that considered the geometry of seed shadows and assumed limited survivors so that the number of juvenile recruits is proportional to the area. Rather than a ratio of 2:1 offspring success from high- versus low-fecundity parents, our model predicted a ratio of 1.42:1, which would translate into weaker selection. Empirical ratios of juvenile offspring and of flowers produced conformed well to the model's prediction. Extending the model shows how spatial relationships of parents and seed dispersal patterns modify inferences about relative fitness based solely on fecundity.

The seagrass Zostera japonica Aschers. and Graebn. occurs as pure populations and in mixture with Zostera marina L. along the intertidal regions of southwest British Columbia. At the Roberts Bank study area seed and seedling dynamics were studied in three vegetation zones: a landward monospecific zone of Z. japonica, a zone of co-existing Z. japonica and Z. marina, and a seaward monospecific zone of Z. marina. Many more seeds were produced than were found in the sediment, and even fewer germinated. Zostera japonica seeds were most abundant in the seed bank in the upper zones where there is high Z. japonica density. Even though seeds remained in the water column for up to two months, very few seeds dispersed into the lower zone populated by Z. marina. thus limiting Z. japonica's colonization of the lower zones. It is unclear what limits the dispersal of Z. japonica seeds. Of the seeds that were incorporated into the sediment few germinated (5% or less). When seeds were planted in buckets placed into the sediment, with and without Z. marina, Z. japonica was able to germinate, grow, and reproduce in one year throughout the study area. Seedlings that emerged earliest (in April) either did not establish or did not survive as long as those seedlings that emerged later in May and June. Seedlings were often found uprooted, floating in the water. The rim of the buckets and the presence of Z. marina shoots appeared to protect the Z. japonica seedlings, preventing uprooting, but the results were not conclusive. Once seedlings became established, they spread vegetatively at a rapid rate and can persist throughout the winter, either as reduced shoots or as overwintering rhizomes. These overwintering plants contribute greatly to the following year's population. / Science, Faculty of / Botany, Department of / Graduate

The purpose of the study was to find alternatives to fences by comparing the frequency of damages on different configurations of chemical treated, mechanically protected and untreated hybrid aspen seedlings, planted on agricultural land. The study also explored whether anthropogenic disturbance (proximity to roads and buildings) had any effect on the frequency of seedlings damages. The study was conducted as a quantitative study with field measurements over the course of a year, in two sample plots with hybrid aspen seedlings planted in May 2016 in Vimmerby kommun, Kalmar län. The field data was compiled into Excel and compared for differences between treatments and proximity to anthropogenic disturbance. The result showed that seedlings closer to anthropogenic disturbance showed a lower frequency of damage. There was no significant difference between the mechanical (Taimitassu) and chemical (Arbinol B) seedling protection. The highest frequency of damages occurred in the summer, peaking in July. In conclusion, when choosing a place to plant hybrid aspen, there should be anthropogenic disturbance to minimize the frequency of browsing.

Euclea natalensis A.DC. belongs to the Ebenaceae family, and is extensively distributed along the eastern coast of southern Africa. Many Euclea species are widely gathered by indigenous people because of their medicinal properties. Roots of these plant species are frequently used to treat respiratory complications such as chest pains, bronchitis, pleurisy and asthma. Ground root powder is topically applied in cases of leprosy and is used by some ethnic groups to treat toothache and headache. The bioactivity encountered is attributable to naphthoquinones, which are common phenolic compounds in the Ebenaceae family. Naphthoquinones isolated from E. natalensis (shinanolone, 7-methyljuglone, diospyrin, isodiospyrin and neodiospyrin) have exhibited a broad spectrum of antimicrobial activity. The demand for these products will escalate due the amount of plant material required to further research. We need to explore techniques that can maximize their productivity. The present study was conducted on E. natalensis, in an attempt to establish if there exists any correlation between the accumulation of naphthoquinones and stages of seedling growth, seasonal fluctuations and application of fertilizers. A possible correlation between seedling growth stages and the accumulation of naphthoquinones (shinanolone, 7-methyljuglone and diospyrin) was investigated in seeds and seedlings of Euclea natalensis. In this study, the seeds represented the first stage, whereas the second seedling stage was defined as the stage when the radicles were about 6 cm long. The lengths of the seedlings at the third, fourth and fifth seedling stages were 9 cm, 12 cm and 16 cm respectively. Plant materials collected from the five seedling stages were separately extracted using chloroform and the naphthoquinones were then quantified by means of High Performance Liquid Chromatography (HPLC). Mobile phase of MeCN: H2O: AcOH (62.5: 32.5: 5) was used as an eluent in an isocratic mode and at a flow rate of 0.8 ml/min. Standard curves of each of the four compounds were obtained by making a series of dilutions in the concentration range of 22.5 µg/ml to 2.25 µg/ml. Ten microlitres of each dilution was injected three times into the HPLC, and the run time for each injection was 20 minutes. Calibration curves were then generated and used for the quantification of each compound. Shinanolone, which was the only naphthoquinone detectible in seeds, accumulated at variable rates (P<0.01) and no trend could be established between its synthesis and seedling growth. The content of shinanolone ranged from 87.5 mg/kg dry weight (dw) in seeds to a high mean value of 1047 mg/kg (dw) during the fourth seedling stage. A significant correlation (P<0.01) was found between the mean concentrations of 7-methyljuglone and seedling growth. 7-Methyljuglone was quantified at a high mean level of 5003 mg/kg during the third seedling stage and was not detected in the seed samples. A positive correlation (P<0.01) was established between the concentration of diospyrin and seedling stages. Diospyrin was detected at an elevated mean concentration of 6182 mg/kg during the fifth seedling stage, which was higher than the other quantified naphthoquinones. Seasonal variation of naphthoquinones (shinanolone, 7-methyljuglone, diospyrin, isodiospyrin and neodiospyrin) was investigated from eleven plants of E. natalensis subsp. natalensis growing in natural populations, over a period of four seasons. The roots were harvested, dried, extracted and analysed as in the previous study. The mean levels of shinanolone and 7-methyljuglone were found to be uniform in all the seasons and no statistically significant variation could be found between seasonal changes and their mean concentrations. Accumulation of isodiospyrin and neodiospyrin varied significantly with seasonal changes (P<0.05). These two bioactive naphthoquinones were detected only in summer and autumn respectively, and not in winter. A statistically significant variation (P<0.05) was established between the levels of diospyrin and seasonal fluctuations. Diospyrin was detected at a mean concentration of 3190 mg/kg (dw) during spring, which was higher than the other naphthoquinones quantified in all four seasons. The effect of NPK fertilizers on growth performance and accumulation of naphthoquinones (shinanolone, 7-methyljuglone, diospyrin, isodiospyrin and neodiospyrin) in seedlings of E. natalensis grown in shade and under field conditions was investigated. Each group was subdivided into four subgroups, which were then subjected to four respective treatments of water-soluble foliar feed (2:1:2 (44) NPK) at three different concentrations. Treatments tested were as follows: Treatment 1 at 40 g/l, Treatment 2 and Treatment 3 at 20 g/l and 10 g/l respectively. The control group received only supplemental water. The first harvest was conducted after 6 months of application of fertilizers and the second one was done after 12 months of treatment. Roots and shoots were harvested and analysed separately. The naphthoquinones were quantified as previously described. The bioactivity of root extracts from seedlings was tested against Mycobacterium smegmatis and extracts with lower MIC were further tested on M. tuberculosis. Growth parameters differed between the two groups, with seedlings from the shadehouse showing more plant vigour than the field grown plants. No significant interaction could be established between the measured growth factors and treatment. A significant interaction (P<0.001) was found between Treatment 2 and shadehouse seedlings. Treatment 2 enhanced vegetative performance with the mean values of fresh weight of shoots and roots being twice as much as their respective control mean values. A significantly positive correlation was established between the concentration of shinanolone (P<0.01), isodiospyrin (P<0.05) and neodiospyrin (P<0.05) with fertilization from field-grown seedlings. Application of NPK fertilizers significantly (P<0.05) increased the accumulation of neodiospyrin in seedlings subjected to shadehouse conditions. The most potent naphthoquinone, 7-methyljuglone, was found to be abundant in all the extracts and was quantified at a high mean concentration of 10200 mg/kg from shadehouse seedlings. Root extracts of E. natalensis seedlings grown under field conditions were generally more active against the bacterial strain of M. smegmatis as compared to extracts acquired from roots of seedlings maintained under a shadehouse setting. A lowest minimum inhibitory concentration (MIC) of 0.78mg/ml against M. smegmatis was observed from the second harvest of field-cultivated seedlings of the control and Treatment 1 subgroups. The MIC values for shadehouse seedlings ranged from 1.6 to 6.3 mg/ml. Minimum bactericidal concentration (MBC) values from all the extracts tested were relatively higher than their respective MIC’s. Root extracts of E. natalensis were more active against M. tuberculosis and their MIC values were lower than the tested concentrations. Extracts acquired from field-grown seedlings were more active against M. smegmatis with a lowest MIC value of 0.78 mg/ml. Extracts from the control group and Treatment 1, which had less application of fertilizers were more active against strains of M. tuberculosis with MIC value of 10 µg/ml. This shows the selectivity of E. natalensis against the mycobacterial strain of M. tuberculosis. Based on the findings, synthesis and accumulation of naphthoquinones in E. natalensis is highly variable within individuals of the species investigated. Naphthoquinones accumulate in relatively higher amounts in roots of E. natalensis than in the aboveground structures, which validate their harvest by indigenous people. The concentration of shinanolone varied slightly and its production increased with seedling growth. The synthesis of 7-methyljuglone is independent of fertilisation as its accumulation was enhanced in seedlings subjected to control treatment. Neodiospyrin and isodiospyrin were always present in every sample obtained from the seedlings but they were not detectible in every profile of samples from mature plants. Diospyrin is the only naphthoquinone that was detected in every sample analysed and also quantified in high concentrations from mature plants harvested in spring. The study showed that depending on the requirement of a particular naphthoquinone for research, one could target the seasons and seedling stages recommended from this study. This study also showed that field-cultivated seedlings produced more potent naphthoquinones than the ones subjected to controlled environments. / Dissertation (MSc (Plant Science))--University of Pretoria, 2008. / Plant Production and Soil Science / unrestricted

Remote sensing of the environment has become an effective and useful research approach applied across a wide range of scientific and professional disciplines. Generally remote sensing is used to evaluate patterns and processes at broad spatio-temporal scales, such as classifying landscape vegetation patterns or for creating digital surface models, however, there are increasing opportunities to expand the use of remotely sensed information to a wider range of applications at variable spatial and temporal scales. In the field of plant seedling and germination research methods are needed to improve plant establishment and restoration monitoring, particularly in areas that have historically low success rates such as in semi-arid and arid rangeland landscapes. The purpose of this research is to assess the efficacy of remote sensing for tracking seedling height, seedling density, and seedling fate, and determine the biotic causes of seedling mortality in a rangeland revegetation site in northwestern Utah. In Chapter 1, we use 28 time-lapse and motion sensing infrared cameras (Reconyx) to measure seedling density and height in fenced and unfenced plots during the initial four months of seedling establishment and growth. We compare imaged-based measurements of seedling height and density with similar measurements collected in the field and at different daylight hours to determine the accuracy and reliability of remotely sensed measurements. We found that the ideal sample periods for capturing the clearest images were at the time the sun passed zenith and shadows were minimized. Average seedling height was 14% lower in image-based versus field estimates. Seedling density was underestimated by approximately 30% when using cameras. Our study establishes that remote sensing of seedlings using time-lapse cameras is a method for seedling research and monitoring in restoration efforts which merits further research and development. In Chapter 2, we track biotic causes of seedling fate using the methods developed in Chapter 1, and compare seedling survival in fenced and unfenced plots. Fencing led to a four-fold increase in the number of seedlings emerged from the soil. Herbivory and damage caused by trampling and burial resulted in the death of 61.4 % of all unfenced seedlings. Fencing plots increased the probability of seedling survival by seven times. Using cameras to track seedling fate at two restoration sites revealed that small herbivores, including Lepus californicus, Thomomys bottae, and Dipodomys sp. drastically reduced seedling survival during the first year after planting. Effects of herbivores on seedling survival should be taken into consideration when planning revegetation operations, and further research can increase knowledge of how herbivory affects restoration efforts. Using cameras can provide meaningful information to managers and researchers about seedling status and fate.

Surface mines in mountainous areas cause environmental deterioration at lower elevations in the watershed. The most successful long term solution to the downstream problem is revegetation. However, mine spoils are low in essential plant nutrients, have low water holding capacity, and are often acidic. These factors limit plant colonization. Plants must also be adapted to the environmental conditions of high elevations. At the McLaren Mine, }1ontana, at 2800 m, it was observed that seedlings on revegetation plots were desiccated, indicating possible water deficits. Field and growth chamber studies were carried out to determine the effects of some spoil ameliorating treatments on leaf water potentials, leaf pressure potentials and seedling development and mortality. Poa alpina L. and Alopecurus pratensis L., the two plant species seeded are successful revegetation species in the area. The four treatments were: 1) a control group with no spoil treatment, 2) peatmoss incorporated into the spoils, 3) a surface mulch of jute net, and 4) peatmoss-plus-jute net. Results showed a decrease in water stress experienced by plants on plots with spoil ameliorating treatments. The jute net, or peatmoss-plus-jute net treatments were the most effective in reducing water stress. Leaf pressure potential data were extremely variable, making it difficult to determine trends. In the field seedling mortality was reduced on plots with jute net or peatmoss--plus-jute net. No trends were apparent in the growth chamber study. In both studies seedlings were larger with jute net or peatmoss-plus-jute net treatments.

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第20443号 / 農博第2228号 / 新制||農||1050(附属図書館) / 学位論文||H29||N5064(農学部図書室) / 京都大学大学院農学研究科森林科学専攻 / (主査)教授 神﨑 護, 教授 北島 薫, 教授 大澤 晃 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

The occurrence of phenotypic and genotypic diversity is the key factor in crop improvement including abiotic stress tolerance. The focal objectives of this study were to evaluate and characterize 74 tropical indica rice breeding lines for phenotypic and genotypic diversity, screening for the most devastating abiotic stresses in rice; drought and salinity at the seedling stage at morpho-physiological and molecular levels. To fulfill these objectives, five studies were conducted in pots; first two experiments aimed at assessing phenotypic and yield variability at seedling and maturity stages respectively; based on several (more than 20) root and shoot traits which exploited a wide range of variability among genotypes for measured traits. Germplasm was then screened for drought stress at two moister regimes, 50%, and 100% moisture levels, under mini-hoop structures. Nine percent of the genotypes exhibited a high tolerance to drought stress, and genotypes IR86638 and IR49830 were identified as the most and least drought tolerant respectively. Germplasm was also screened for salinity tolerance in pure sand pot-culture (a simple, efficient and alternate screening method) at three levels; high salt stress (EC 12 dSm-1), moderate salt stress (EC 6 dSm-1), and control imposed one week after emergence. Thirteen genotypes (17.57%) were identified as highly salt tolerant; genotypes FED 473 and IR85427 were highly salt tolerant and salt sensitive, respectively. Root traits were found more crucial and best descriptors in identifying both salinity and drought tolerant genotypes. Genotypes were further used in Genome-wide Association Study (GWAS) to uncover important SNPs, QTLs or genes related to salinity tolerance. A higher number of significant SNPs were discovered for root traits, indicting the importance of root traits in identifying abiotic stress tolerance in rice. The knowledge gained from this investigation could be useful in breeding for better crop establishment, yield improvement, screening for any abiotic stress tolerance.