近年來房價持續攀升，政府決定實施特種貨物及勞務稅條例以降低投機買賣之行為，達到防止房價泡沫化，平抑房價之目的。為探討資本市場對該條例之預期，本論文首先以事件研究探討特種貨物及勞務稅條例消息公布之際，上市櫃建設公司是否產生負的累積異常報酬率，並採用迴歸模型探討公司特性與該公司受特種貨物及勞務稅條例影響幅度的關聯性。本論文進而透過對上市櫃建設公司營業收入及營業毛利率在特種貨物及勞務稅條例消息公布後是否產生不利影響之探討，了解特種貨物及勞務稅條例消息公布對建設公司銷售面之實質影響，最後採用迴歸模型探討公司特性與建設公司銷售面受影響幅度之關聯性。 本研究研究結果發現： 1、在特種貨物及勞務稅條例消息公布之相關事件日，上市櫃建設公司普遍均產生顯著的負向股票異常報酬率。 2、股票累積異常報酬率與公司特性關聯性之迴歸結果顯示，研議及開徵特種貨物及勞務稅消息公布之事件日，存貨比率較高、公司規模較大、公司成長性較高之上市櫃建設公司，其累積異常報酬率較低。 3、在特種貨物及勞務稅條例消息公布後，上市櫃建設公司之營業收入產生不利影響，但對營業毛利率並無顯著之不利影響。 / In order to curb the housing price hike and prevent housing bubble, Taiwan government implemented the Specifically Selected Goods and Services Tax Act. The Act is expected to have negative impacts on stock market returns of construction companies and real estate market. Using event study method, this study examines the stock market reactions to the announcements of the legislature policy. This study explores the relation between the stock cumulative abnormal returns (CAR) of the Act and firm characteristics. This study finds that the stock market reacts negatively when the news of the Act are released. The regression results indicate when the news of the Act are released, for construction companies, CARs are negatively related with inventory-to-total assets ratio, corporate size and growth. This study also finds the construction companies’ revenue reflected the negative impacts on real estate market.

The imidazoline I1 receptor (I1-R) is a novel receptor found primarily in the brain and nervous tissue where it modulates neurotransmission. It is named for its high affinity for compounds with an imidazoline structure such as the anti-hypertensive drugs, clonidine and moxonidine. The imidazoline receptor antisera-selected protein (IRAS) is the putative clone of the I1-R. IRAS has a unique structure, which does not resemble any other receptor protein. IRAS is present throughout the body with highest levels in the brain. There is a growing body of research examining the physiological roles of IRAS as an I1-R, in cell survival, migration and protein trafficking. However, there is little research into its neuronal functions. IRAS interacts with other membrane receptors: the mouse homologue of IRAS reorganises the actin cytoskeleton through interaction with the α5β1 fibronectin receptor. IRAS also binds insulin receptor substrate 4 and enhances insulin-induced extracellular signal-regulated kinase1/2 (ERK1/2) activation. Actin reorganisation and ERK1/2 activation are important for the development of neurites during neuronal differentiation. Therefore, the work described in this thesis aimed to investigate the effects of IRAS on neuronal differentiation. Studies reported in this thesis also aimed to investigate whether IRAS affected ERK1/2 signalling of other receptors involved in neuronal differentiation such as the NGF receptor, TrkA, and lysophospholipid receptors. The above aims were carried out in neuronal model PC12 cells transfected with either IRAS or a vector plasmid. Fluorescence microscopy and Western blotting techniques were used to examine the effect of IRAS on cell morphology and ERK1/2 signalling. The work described in this thesis found that IRAS reorganises the actin cytoskeleton and enhances growth cone development in PC12 cells. This study also shows that IRAS differentially enhances or inhibits NGF-induced PC12 cell differentiation depending on the presence or absence of serum in the media. In full-serum conditions, IRAS enhanced neurite outgrowth and this was accompanied by an increase in ERK1/2 activation. In serum-starved cells, IRAS inhibited neurite outgrowth with similar levels of ERK1/2 activation observed in vector- and IRAS-transfected cells. Finally, studies presented in this thesis found that IRAS enhances lysophosphatidic acid-induced ERK1/2 activation and that IRAS interacting with lysophospholipid receptor agonists present in serum is a potential mechanism by which it enhances NGF-induced ERK1/2 activation in full-serum conditions. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1345359 / Thesis (Ph.D.) - University of Adelaide, School of Medical Sciences, 2008

abstract: The federal No Child Left Behind Act has set the goal that all students in every state shall be proficient in reading by 2014. Arizona teachers face the challenge of having 100 percent of their students meeting or exceeding grade level reading standards assessed by Arizona's Instrument to Measure Standards (AIMS). One of my goals as a reading teacher is to widen the range of options my students will have. My goal every year is to have my students read at or above grade level. I also am committed to inspiring students to become motivated to love literacy because voluntary lifelong reading is important in peoples' lives. The purpose of this study was to investigate conducting brief, interactive, weekly reading conferences during daily Scaffolded Self-Selected Reading (ScS-SR) sessions while incorporating Transactional Strategy Instruction with a pair-share with a partner, and to see if it would improve my students' reading motivation and comprehension. Data were collected via the Motivation to Read Profile Survey and Interview, informal observations, Dynamic Indicators of Basic Early Literacy Skills Oral Reading Fluency Test (DIBELS: ORF), and Oral Reading Records used by my school district. Findings concluded that students tended to become more willing readers, with several of them explicitly attributing their newfound willingness to read to my efforts. Most students became somewhat more aware of their reading experiences, explaining how different types of books in ScS-SR affected them. All students' reading comprehension performance improved, with measureable increases in students' instructional reading levels, retellings, and meaningful miscues that students attributed to leveled books, strategy instruction, and retellings. / Dissertation/Thesis / Ed.D. Leadership and Innovation 2011

Based on analysis build the profile of domestic and foreign visitor in selected area of Lipno. A partial aim of this thesis is to make a methodology for the profile of domestic and foreign visitors. The investigation was carried out between December 2010 and January 2011 in selected area of microregion. As the method was chosen observing and questioning. The chosen territory means the area of Lipno Ski Resort and the village Lipno nad Vltavou and Frymburk. This area was chosen deliberately, since there is the necessary equipment for winter sports and because there are the greatest concentration of visitors in the winter. The increasing number of visitors during the last few winter seasons is the reason for the implementation of the questioning is in this season. The outcome of the thesis is the profile of domestic and foreign visitor in selected area of Lipno and the methodology of compiling the profile of domestic and foreign visitors in any chosen area.

La maladie de Lyme, ou borréliose de Lyme, est une infection bactérienne causée par le spirochète Borrelia burgdorferi sensu lato et transmise à l’hôte (homme, animal) par piqûre de tique du genre Ixodes. Cette maladie, caractérisée par un polymorphisme clinique important, est la maladie à transmission vectorielle la plus répandue dans l’hémisphère nord. Un traitement par antibiotiques permet une guérison rapide, mais si la maladie est diagnostiquée tardivement, certains symptômes persistent. Actuellement, aucun vaccin n’est commercialisé pour l’homme. Dans ce contexte, nous avons développé des approches protéomiques afin d’apporter de nouveaux éléments de compréhension du mécanisme d’interactions de la triade tique / bactérie / hôte. Ces travaux, visant particulièrement le développement de nouvelles stratégies vaccinales et diagnostiques, sont articulés autour de trois parties : - L’identification, suite à de nombreuses optimisations, d’une méthode d’analyse HPLC-UV et nanoLC-MS/MS, de protéines présentes dans des extraits de glandes salivaires de tiques et possédant une activité sur la réponse immunitaire innée cutanée. Ces développements ont mis en évidence une liste restreinte de protéines potentiellement bioactives. - La mise au point, sur un modèle murin, d’une méthode de détection d’une protéine de Borrelia burgdorferi, OspC, dans des biopsies cutanées par spectrométrie de masse ciblée LC-SRM. Cette étude a ouvert des perspectives quant au développement de nouveaux outils diagnostiques. - L’évaluation de la faisabilité de la détection de Borrelia burgdorferi directement à la surface de la peau par imagerie par spectrométrie de masse MALDI-MSI. / Lyme disease, or Lyme borreliosis, is a bacterial infection caused by Borrelia burgdorferi sensu lato and transmitted to the human or animal host by an Ixodes tick bite. This disease, characterized by a huge clinical polymorphism, is the most common vector-born disease in the Northern Hemisphere. An antibiotic treatment allows a fast recovery, but if it is diagnosed too late, some symptoms persist. Currently, no vaccine is marketed for humans. In this context, we have developed proteomic approaches to bring new understanding to the interaction mechanism of the triad tick / bacteria / host. This work, aimed particularly at the development of new vaccinal and diagnostic strategies, has three parts: - Identification, after numerous optimizations, of the analytical method HPLC-UV and nanoLC-MS/MS, of proteins that are present in tick salivary gland extracts and having activity on cutaneous innate immunity response. This work has highlighted a list of proteins with a potential biological activity. - Development, with a murine model, of a method for detecting Borrelia burgdorferi protein, OspC, in cutaneous biopsies by targeted mass spectrometry LC-SRM. This study has opened up perspectives concerning new diagnostic tools. - Evaluation of the feasibility of the Borrelia burgdorferi detection directly on the skin surface by MALDI imaging mass spectrometry.

nÃo hà / Este trabalho consiste no estudo das caracterÃsticas fÃsicas de imÃveis localizados em bairros selecionados da cidade de Fortaleza, as quais sÃo determinantes para a formaÃÃo de preÃo. Tendo por base a metodologia de preÃos hedÃnicos ou preÃos implÃcitos, sÃo investigadas as caracterÃsticas do bem habitacional que influem na formaÃÃo do seu preÃo final e na determinaÃÃo da cesta Ãtima de bens do consumidor. Com base em uma pesquisa de campo sobre as caracterÃsticas de 607 imÃveis construÃdos no perÃodo de 2007-2012, especificou-se um modelo economÃtrico de preÃos hedÃnicos para se identificar os efeitos de um vetor de caracterÃsticas fÃsicas sobre o preÃo final do imÃvel. Concluiu-se que as variÃveis estudadas no vetor de caracterÃsticas se mostraram estatisticamente relevantes na equaÃÃo estimada para explicar a formaÃÃo dos preÃos dos imÃveis residenciais, dentre as quais se destacam: Ãrea privativa, acabamento, nÃmero de pavimentos, presenÃa de elevador, vagas de garagem, idade do imÃvel. Do modelo proposto, ao se avaliar apartamentos novos e usados, desconsiderando aqueles em fase de construÃÃo, deduz-se que, dentro do perÃodo de referÃncia, deve optar-se pela compra de um imÃvel usado a fim de obter-se maior ganho esperado de capital. AtravÃs dos dados apresentados, tornou-se tambÃm possÃvel estabelecer o comportamento do consumidor neste perÃodo, no qual prefere adquirir imÃveis novos para moradia e usados para investimento. / This work investigates the physical characteristics of properties located in selected neighborhoods in the city of Fortaleza, which are decisive for pricing. The methodology of hedonic or implicit prices is applied to determine the property characteristics that influence the achievement of optimal bundle of other goods and housing made by the consumer for forming its demand price. Based on a field survey on the characteristics of 607 buildings constructed in the period 2007-2012, it was specified an econometric model of hedonic pricing to identify the effects of a vector of physical characteristics on the final price of the property. It was concluded that the coefficients of the variables in the vector of characteristics were statistically significant in the estimated equation to explain the formation of the prices of residential properties, among which are: private area, quality of materials, number of floors, availability of elevators, garage spaces and years of construction. By contrasting brand new and other apartments, excluding those under construction, it follows that the latter should be chosen by the consumers if higher expected capital gain is the target. Yet, it is also established that the consumer behavior is such that they prefer to acquire new properties for own housing and used ones for investment.

Camundongos foram selecionados geneticamente para alta (AIRmax) ou baixa (AIRmin) resposta inflamatória aguda, sendo utilizados em experimentos de regeneração tecidual. Foi observado que AIRmax apresentam uma regeneração rápida do tecido da orelha em relação aos AIRmin, sugerindo a existência de loci reguladores comuns para ambos fenótipos de inflamação e regeneração do tecido. Neste estudo investigamos o fenótipo inflamatório e o perfil de expressão gênica global em AIRmax e AIRmin durante a fase inicial da regeneração do tecido da orelha. A análise histológica mostrou que AIRmax apresentam uma regeneração completa, com formação de ilhas de cartilagem e glândulas sebáceas no centro da área regenerada. Os AIRmin não apresentam regeneração. Os dados obtidos sobre edema de orelha e níveis de MPO foram maiores em AIRmax quando comparados com AIRmin (P <0,001). A análise de expressão gênica global mostrou 794 genes ativados e 528 genes reprimidos em AIRmax, enquanto 1.086 genes ativados e 1.145 genes reprimidos nos AIRmin, 48 horas após a lesão. AIRmax e AIRmin apresentaram alta modulação de genes sobrerrepresentados em temas biológicos para resposta inflamatória, adesão celular e quimiotaxia (Gene Ontology). No entanto, foi observado uma baixa modulação de genes sobrerrepresentados para o transporte em AIRmax e para taxia, contração muscular e ciclo de ubiquitina em AIRmin. Nas regiões próximas ao QTL anteriormente encontrado no cromossomo 1, foram observados genes diferencialmente expressos como Stat1, Casp8, Hspe1 e Il1r2, enquanto Lect1, Fndc3 e Egr3 foram detectados no cromossomo 14. Os experimentos com qPCR mostram uma alta expressão de Il-1<font face=\"Symbol\">b, Il-8rb e Mmp9 em AIRmax e Cxcl2, Tnfa e Tgfb em AIRmin. Concluímos que os animais AIRmax e AIRmin apresentam (nos cromossomos 1 e 14) genes inflamatórios diferencialmente expressos que podem estar envolvidos nos fenótipos de resposta inflamatória aguda e regeneração do tecido da orelha. / Mice selected for high (AIRmax) or low (AIRmin) acute inflammatory response were used in tissue regeneration experiments. It was observed that AIRmax present faster ear tissue regeneration than AIRmin mice, suggesting the involvement of common regulatory loci for both inflammation and ear tissue regeneration phenotypes. In the study we investigated some inflammatory phenotypes and global gene expression profiles in AIRmax and AIRmin mice during the initial phase of the ear tissue regeneration. The histological analyses showed that AIRmax regeneration was complete, and cartilage islands and sebaceous glands were formed in the middle of the regenerated area. AIRmin mice displayed not regeneration. Ear thickness oedema and MPO levels were higher in AIRmax than AIRmin mice (P< 0.001). Global expression analysis showed 794 activated and 528 repressed genes in AIRmax, while 1086 activated and 1145 repressed genes were observed in AIRmin mice 48 hours after injury. AIRmax and AIRmin mice presented up-regulated genes over-represented in inflammatory response, cell adhesion and chemotaxis biological themes (Gene ontology). However, down-modulated genes were significant over-represented for transportation in AIRmax and for taxis, muscle contraction and cycle ubiquitin in AIRmin mice. In the previously QTL regions detected on chromosome 1 differentially-expressed Stat1, Casp8, Hspe1 and Il1r2 genes were found, while Lect1, Fndc3 and Egr3 were detected on chromosome 14. qPCR experiments showed high expression of Il-1<font face=\"Symbol\">b, Il-8rb and Mmp9 in AIRmax and Cxcl2, Tnfa, and Tgfb1 in AIRmin mice. We conclude that AIRmax and AIRmin mice presented several (some on chromosomes 1 and 14) differentially-expressed inflammatory genes which could be involved in the acute inflammatory response and ear tissue regeneration phenotypes.

Sublinhagens de camundongos AIRmax e AIRmin homozigotas para os alelos S do gene Slc11a1 diferem na capacidade de reparar um orifício na orelha. Os animais AIRmaxSS regeneraram o tecido da orelha 30 dias após a perfuração, enquanto que os animais AIRminSS apenas cicatrizaram a ferida mas nunca a restauraram completamente. A regeneração da orelha observada nos animais AIRmaxSS deve-se a inflamação menos intensa da área ferida no início do processo de reparação, culminando em baixa deposição de colágeno e expressão da proteína a-SMA na orelha desses animais e a repressão de genes participantes em funções relacionadas à contração muscular. Além disso, detectamos alguns genes candidatos no cromossomo 11 regulando o fenótipo de cicatrização tissular da orelha de camundongos AIRminSS. Os resultados obtidos sugerem que o grau da resposta inflamatória, assim como a ativação ou repressão de genes participantes durante os eventos de reparação tissular podem modular a qualidade da resolução da injúria, culminando em um processo regenerativo ou de cicatrização. / Homozygous AIRmax and AIRmin sublines for Slc11a1 R and S alleles differ in the ability to repair to the ear hole. AIRmaxSS mice exhibited regeneration 30 days after ear punch while AIRminSS animals did not show regeneration. The regeneration observed in AIRmaxSS mice was due to lower inflammation at the beginning of repair process resulting in less deposition of collagen and expression of a-SMA protein in the ears of these animals. Down regulated genes related with muscle contraction was observed in AIRmaxSS mice. In addition, AIRminSS mice presented gene cluster on chromosome 11 with expression profile that predispose them to wound healing with scar. These results suggest that the importance of regulating inflammation in the initial events and the activation and repression of some genes related to the wound healing phenotype can drives tissue regeneration or wound healing after ear punch.

Les matériaux carbonés présentent de nombreux avantages pour les domaines des nanotechnologies et de l'environnement.La mixité de chiralité des nanotubes de carbone limite leur application dans les appareils électroniques et le nano-confinement. Dans une première partie, ce travail de thèse s'est concentré sur la séparation en chiralité de nanotubes de carbone de type CoMoCAT, afin d'élaborer de nouveaux nano-conteneurs.Après sélection en chiralité, nous avons évalué les propriétés sous hautes pressions des fagots de nanotubes sélectionnés, et leur interaction avec l'eau. Les résultats ont montré que les fagots supportent des pressions jusqu'à 17 GPa, avant de subir un effondrement radial réversible, permettant de les utiliser en tant que nano-enclumes.L'élaboration d'une éponge de carbone (super)hydrophobe pour le traitement des eaux après pollution aux hydrocarbures a été décrite dans une seconde partie. La pyrolyse de mousses polymères a permis de conserver la très grande porosité de la mousse (> 99%), tout en lui conférant des propriétés proches de la superhydrophobie et de grandes capacités d'absorption de pétrole et solvants organiques (85-200 g/g). L'élasticité du matériau permet sa régénération par simple compression mécanique : récupération du polluant et réutilisation de l'absorbant. De plus, cette caractéristique reste valable même après une centaine de cycles de compression-décompression, en conservant 81% de sa capacité d'absorption dans le cas du pétrole brut / Carbon materials present many advantages for the nanotechnology and environment fields. The chirality mixity of carbon nanotubes limits their application in electronic devices and the nano-confinement. In the first part, this thesis has focused on the chirality separation of CoMoCAT carbon nanotubes, in order to elaborate new nano-containers.After the chirality selection, the properties of selected nanotubes bundles under high pressure were evaluated, as well as their interaction with water. The results show that the bundles support pressures until 17 GPa, before to undergo a reversible collapse, allowing their application as nano-anvils.The (super)hydrophobic carbon sponge elaboration for the clean-up of water polluted by oils was described in the second part. The polymeric foams pyrolysis allows to keep the very high foam porosity (> 99%), to give properties next to the superhydrophobicity and large absorption capacities in oils and organic solvents (85-200 g/g). The material elasticity allows its regeneration by simple mechanical compression : recovery of pollutant and re-use of the absorbant. Moreover, this feature remains valid after a hundred compression-decompression cycles, with 81% of the initial crude oil absorption capacity

The subject of this thesis is the analysis of unit linked insurance in the insurance market of Czech Republic. United linked insurance is presented as a successful combination of risks coverage and savings unit. We are talking about mixed insurance in case of death or survival exact age with single or normal insurance premium, where the size of indemnity depends on yields from investments. It is very complicated insurance product with many benefits and disadvantages. The thesis is divided into more chapters. The first chapter is devoted to determination of the concept and classification of unit linked insurance. In the second one I describe the situation and development trends in the field of unit linked insurance in Czech insurance market. Further chapters involve specific aspects and problems of the product, actual situation in regard of financial crisis and anticipated future development.