Yes / Turbulence-induced secondary currents are commonly present in straight natural as well as artificial open channels without bed forms. Different structures of cellular secondary currents can be seen in open-channel flows due to various bed configurations. In our study, mathematical models of turbulence-induced secondary currents in the vertical and transverse directions within a straight open rectangular channel with alternate rough and smooth longitudinal bed strips are proposed. The proposed models are derived using appropriate theoretical and mathematical analysis. Most of the previous models of secondary currents in the literature are proposed empirically and without proper mathematical derivations. The effects of fluid viscosity and eddy diffusivity are included in the present study to make it more practical. Initially, the governing equation for vertical secondary flow velocity is derived from continuity and the Reynolds-Averaged Navier Stokes equations. Then, the proposed problem is divided into two sub-considerations, corresponding to the base flow and perturbed flow. Finally, these sub-problems are analytically solved using method of variables separation with suitable boundary conditions. Different models to consider two different types of bed-roughness configurations (i.e. equal and unequal lengths of smooth and rough longitudinal bed strips) are obtained. Apart from velocity formulations, models of the stream function are proposed for these two types of bed configurations. All proposed models are validated using existing experimental data for the various bed configurations in open-channel flows and satisfactory results have been obtained. These present models are also compared with empirical models from the literature and they are found to be more effective in representing both types of bed-roughness configurations. The effects of bed configuration on the streamlines of settling velocity are also investigated. Results show that laterally-skewed secondary cells (which occurs due to unequal smooth and rough bed strips), have significant effects on the closed ω-streamlines in terms of shape and location of the centre of these streamlines. More precisely, it is found that the area of the downflow zone proportionally increases with the length of rough-bed strips.

Studies were conducted to determine the role of bioflocculation in the activated sludge unit processes. Laboratory and full-scale studies revealed that bioflocculation is important in determining settling, dewatering, effluent and digested sludge properties (activated sludge properties) and may be vital to the function of all processes related to the above properties. In these studies, it was shown that divalent cations such as calcium and magnesium improved activated sludge properties, whereas monovalent cations such as sodium, potassium and ammonium ions were detrimental to these properties. The divalent cations promoted bioflocculation through charge bridging mechanisms with negatively charged biopolymers (mainly protein and polysaccharide). It was found that oxidized iron plays a major role in bioflocculation and determination of activated sludge properties through surface interactions between iron and biopolymers. Oxidized iron was effective in removing colloidal biopolymers from solution in coagulation and conditioning studies. The research included experiments evaluating effects of potassium and ammonium ions on settling and dewatering properties; effects of magnesium on settling properties; effects of sodium, potassium, calcium and magnesium on effluent quality; effect of solids retention time on effluent quality; and evaluation of floc properties during aerobic and thermophilic digestion. A floc model is proposed in which calcium, magnesium and iron are important to bioflocculation and the functionality of aeration tanks, settling tanks, dewatering equipment and aerobic or anaerobic digesters. It is shown that activated sludge floc properties affect wastewater treatment efficiency. / Ph. D.

The possible effects of changes in the feeding pattern on activated sludge properties related to bioflocculation have been analyzed in lab scale sequencing batch reactors (SBR) in order to determine if these changes in effluent water quality and settling and dewatering properties are significant, so they can be considered in future studies or if they can be recommended as crucial when operating and designing wastewater treatment plants. The activated sludge process is widely used to treat wastewater from both industrial and municipal sources. Biomass from industrial facilities containing high monovalent to divalent ion content usually settles poorly, which leads to low quality effluents that fail to meet environmental requirements. Therefore, the combined effect of feeding pattern plus the addition of sodium to activated sludge reactors was studied in this experiment. A series of SBRs were operated at different sodium concentrations that ranged from 1.5 - 15 meq/L and different feeding times that ranged from 1 minute to 4 hours. Biomass samples were taken from each reactor to study the settling and dewatering properties and effluent samples were used to analyze the amount of organic matter and exocellular polymeric substances present due to deflocculation. As expected, the changes in feeding strategies affected all of the properties measured. When the feeding time was maintained low (pulse feed) the effluent quality and settling properties were the best. As the feeding time was increased the effluent quality, settling, and dewatering properties increased suggesting that the way in which the reactors were fed affected the overall bioflocculation process. The causes of the high deflocculation observed are not well understood, but data suggest that a microbial community change could have affected exocellular biopolymers which are believed to play an important role on bioflocculation. This research demonstrates the importance of the interaction between cation content and feeding pattern when operating a wastewater treatment plants and when reporting lab-scaled results related to settling and bioflocculation. / Master of Science

The brown marmorated stink bug (Halyomorpha halys) (Stål) is known for overwintering in human homes. Studies were conducted to understand the behavior of adult bugs in response to characteristics of potential overwintering structures including gap sizes, light, and presence of dead conspecifics. In a test where bugs were placed in increasingly tight boxes, most bugs settled in gaps 4.5 - 5.5 mm high. None settled in a space less than 3.5 mm high. In boxes with constant tightness, bugs tended to move to the back, and primarily settled along walls and in corners. In both box types, bugs tended to turn and face the cavity entrance during settling. In tests of responses of disturbed overwintering bugs to common household light bulbs over the course of a year, bugs were taken from shelters and exposed to lit bulbs. Bug responses were seasonal, with attraction to light bulbs in spring and summer, but little or no consistent response in fall or winter. The lack of response occurred more often at night than in afternoons. Because not all bugs survive the winter, corpses can accumulate. Single shelter-seeking H. halys were found not to respond to the presence of dead aggregations from the previous years unless touch was possible, in which case aggregations were joined. Dead aggregations from the same year had a repellent effect. Presence of a single dead bug from the same year did not provide any survivorship advantage to overwintering bugs. Results suggest possible improvements to trap shelters. / Master of Science in Life Sciences / The brown marmorated stink bug (Halyomorpha halys) is known for overwintering in human homes. This research investigated the responses of these bugs to some characteristics of overwintering structures, including crevice tightness preference, light sources, and dead bugs leftover from previous years. Tightness experiments indicated that bugs in crevices with hard walls prefer to settle in spaces between 4.5 and 5.5 mm high. Bugs also tended to stay on the floor, go back as far as they could, and face the entrance of the crevice. These findings will help improve overwintering box designs for collection and research, and will help pest control professionals focus their efforts. When bugs were disturbed and exposed to common household light bulbs, they tended to move toward the bulbs in late summer and in spring, but responded less in fall and winter, and responded more often in the afternoon than at night. Bugs were also exposed to other dead bugs in several ways. When lone bugs were exposed to groups of dead bugs from previous years, they went to them only if they could touch them. Single dead bugs had less of an impact. Exposure to groups of dead bugs from the same year drove live bugs away. Overwintering brown marmorated stink bugs do not appear to eat other bugs that have recently died. These results suggest several possible improvements to the designs of trap shelters used for research and control.

Three rain events were sampled from a storm drain at Manassas Mall in Manassas, Virginia. The urban runoff samples obtained were placed into lab-scale Plexiglas~ settling columns to monitor removal of total hydrocarbons (THC) by extended quiescent settling. Samples were collected from the columns at specific depths and times over the 48-hour settling period. The samples were analyzed for total hydrocarbon content on a Horiba oil content analyzer. Hydrocarbon values were averaged at each column depth in order to construct average THC concentration and average THC percent removal profiles over settling time. Maximum average THC removals were 77.8%,32.5%, and 73.6%, respectively, for Storms #1, 2, and 3 after 48 hours of quiescent settling. These average removals corresponded to depths of -2 feet, -1 foot, and -3 feet in the 5-foot tall columns. According to traditional sedimentation theory, pollutants settle out of water to the bottom of the container of interest. This did not appear to be solely the case in this hydrocarbon sedimentation study. It appeared that sedimentation was not the sole removal mechanism at work. Some fractions of oil and grease seemed to reorganize into low-density sub-groups and float to upper regions of the column. Also, the majority of THe removal occurred within the first 18 hours of settling for two of the three storms sampled. / Master of Science

The effect of solids retention time (SRT) or sludge age on activated sludge properties and effluent quality was investigated using laboratory scale reactors. It was found that an increase in SRT resulted in an increase in effluent solution polysaccharide, with the < 3,000 daltons (3K) size fraction contributing up to 68 percent of solution polysaccharides. The feed consisted of low molecular weight, readily degradable protein, suggesting that the observed increases in protein and polysaccharide were due to increased release of exocellular microbial product (EMP). The increase in solution protein and polysaccharide resulted in an increase in effluent chemical oxygen demand (COD). The increase in effluent COD was not accompanied by a similar increase in effluent biological oxygen demand (BOD), indicating that the EMPs released were resistant to biodegradation. At the highest SRT, the resistance to shear decreased and the capillary suction time (CST) increased. Following an initial increase, the sludge volume index (SVI) decreased at higher SRT. / Master of Science

Mud makes up a large fraction of sediment transported within rivers to the coasts. Predicting where mud will settle is complicated by the cohesive nature of silts and clays, which can combine to form larger aggregates known as flocs. The size and density, and consequently, the settling velocity, of flocs is highly dynamic and depends on factors such as turbulence levels within the flow and biogeochemical components of the water and sediment. To better predict where mud will deposit, more observations of flocs while in their natural environment is required to better understand the controls on when, where, and to what degree mud is flocculated. However, the need for more field observations is complicated by the dynamic and fragile nature of flocs. This necessitates the need for developing in-situ observation methods to ensure that measured floc sizes are representative of their in-situ size, and not a result of sampling methods. In this thesis, a new instrument for in-situ observation of flocs is presented. In addition, two methods using the data collected from the instrument allow the user to: (1) identify sand within the particle data using a machine learning algorithm, and (2) estimate the mass suspended sediment concentration of the mud and sand fractions of suspended sediment independently. Results from using the instrument in the lowermost Mississippi River reveal differences in floc sizes over the water column, and by season. In addition, a unique observation of flocs in the presence of a salt wedge is presented. Overall, the instrument provided the first known observations of flocs within the Mississippi River, and provides a start to better understanding controls on floc sizes within the fluvial environment. / Master of Science / Flowing water within large rivers carries sediments such as sand and mud to the coasts. Some of the larger sediment carried by rivers can fall to the riverbed if the river does not have enough energy to carry it in the flow. The remaining sediment can be carried to the coasts where it will fall to the bed, providing the material necessary for estuaries or deltas to form and grow. Understanding when and where sediment falls to the bed within rivers, estuaries, and deltas, allows scientists and engineers to predict how these landforms will change over time to better manage them under future climate conditions. Predicting where mud will fall to the bed is particularly difficult because mud has the ability to stick together to form larger aggregates. These aggregates, known as mud flocs, are constantly changing in size depending on the energy in the river and water conditions. As the mud flocs change in size, the speed at which they fall to the bed changes. As such, observing mud flocs while they are in their natural environment is required to understand the conditions under which they form and change in size. This thesis presents a new instrument that can be used to collect images of mud flocs while they are in their natural environment. Results from using the instrument to observe mud flocs in the lowermost Mississippi River are then presented. This new instrument, and observations of mud flocs made with it, provides new insight into mud floc size within the lowermost Mississippi River.

This dissertation aims to better understand how floc aggregate characteristics and behaviors are modified under different local conditions and how such alterations impact the floc settling velocity, which is one of the most crucial parameters influencing sediment transport modeling. A series of laboratory experiments were conducted to examine the impact of suspended sediment concentration, mixes of clay and silt, and resuspension process to equilibrium floc size and floc settling velocity. In order to observe floc size evolution, a new floc imaging acquisition was first developed. This new method allows flocs in suspended sediment concentration up to C = 400 mg/L can be imaged non intrusively. This new method was applied in all three individual studies, which are composed of this dissertation. The first chapter investigates the behaviors of flocs under constant and decay suspended sediment concentrations within a steady turbulent suspension. In the constant-concentration set of experiments, floc size time series were measured for 12 h for each of the concentration C = 15, 25, 50, 100, 200, 300, and 400 mg/L. In the decay-concentration experiments, clear water was introduced to the mixing tank, simultaneously the suspension was drained out of the mixing tank at the same rate to make the suspended sediment concentration reduce while the turbulent shear was remained unchanged. The data shows that the equilibrium floc size is a weak, positive function of concentration. For example, in order to increase 20% of floc size (approximate 22 um) the concentration needs to be increased by 700% (going from 50 to 400 mg/L). The data also illustrates that during the decrease of concentration from C = 400 to 50 mg/L, the floc size responses to the changes of concentration in the order of 10 min or less. The second chapter examines how silt particles and clay aggregates interact in a turbulent suspension. Floc sizes and settling velocity of three different suspensions, i.e., pure clay, pure silt, and a mixture of clay and silt, were monitored. The floc size data show that the presence of silt particles does not have significant impacts on clay aggregate sizes. Silt particles, however, get bound up within floc aggregates, which in turn increase the settling velocity of the floc by at least 50%. The third chapter examines whether any changes in floc properties during the deposition and resuspension processes. The floc sizes and shapes in a set of experiments with different consolidation times, concentrations, and shear patterns were measured. The conditions at which the flocs deposited or resuspended were maintained the same. The data reveal that floc size and shape of freshly deposited and after resuspended are unchanged. The erosion rate and concentration is a function of consolidation time and the applied shear stress during the deposition phase. Hence, there is a small reduction in resuspended concentration resulting in a slight decrease in resuspension floc size since floc size is also a function of concentration. / Ph. D. / Sediment transport is a narrative poem from mother nature telling us about the evolution of ancient and modern rivers, deltas, and estuaries. For thousands of years, mankind has been examining the coarser part of the poem, the gravel and sand. The finer part, the mud, has not been systematically investigated until the last 60 years. The key difference between sand and mud is the capability of mud to aggregate and form flocs which have sizes, densities, and shapes that are vastly different from the original constitutive particles. This flocculation process adds a layer of dynamics to the erosion, deposition, and transport of mud that is not present in the transport of sand. Therefore, the primary motivations for this dissertation are 1) to better understand the behavior of floc size under different conditions, e.g., in the estuaries, and 2) to provide high-quality data of floc characteristics and size evolution for model development, testing, and calibration purposes. Laboratory studies are conducted to measure the floc size and in some cases settling velocity, as a function of time under different turbulent, concentration, and sediment mixture. The findings in this dissertation help to fill the gaps of knowledge in cohesive sediment transport processes. This dissertation also suggests how floc behaviors should be accounted for under different conditions. Such information is valuable for projects such as management of sediment supplies, mitigation of land loss, restoration, and land-building diversions, e.g., on the Mississippi and Atchafalaya Rivers. Data associated with this dissertation are also available on GitHub under https://github.com/FluidSedDynamics.

Novel settling characteristic metrics were developed based on the fundamental mechanisms of coagulation, flocculation, and settling. The settling metrics determined parameters that are essential in monitoring and optimizing the activated sludge process without the need for expensive or specialized equipment. Current settling characteristic measurements that don't require specialized instruments such as sludge volume index (SVI) or initial settling velocity (ISV), have no fundamental basis in solid-liquid separation and only indicate whether settling is good or bad without providing information as to limitations present in a sludge matrix. Furthermore, the emergence of aerobic granulation as a potential pathway to mitigate solid-separation issues further stresses the need for new settling characteristic metrics to enable integration of the new technology with the current infrastructure. The granule or intrinsic aggregate fraction in different types was of sludge was quantified by simulating different surface overflow rates (SOR). The technique named Intrinsic Settling Classes (ISC) was able to separate granules and floc by simulating high SOR values due to the lack of a flocculation time needed for granules. The method had to be performed in a discrete settling environment to characterize a range of flocculation behavior and was able to classify the granular portion of five different types of sludge. ISC was proven to accurately (±2%) determine the granule fraction and discrete particle distribution. The major significance of the test is its ability to show if a system is producing particles that will eventually grow to become granules. This methodology proved to be very valuable in obtaining information as to the granular fraction of sludge and the granular production of a system. Flocculent settling (stokesian) was found to be predominant within ideally operating clarifiers, and the shift to 'slower' hindered settling (non-stokesian) causes both failure and poor effluent quality. Therefore, a new metric for settling characteristics was developed and classified as Limit of Stokesian Settling (LOSS). The technique consisted of determining the total suspended solids (TSS) concentration at which mixed liquor settling characteristics transition from stokesian to non–stokesian settling. An image analytical technique was developed with the aid of MATLAB to identify this transition. The MATLAB tool analyzed RGB images from video, and identified the presence of an interface by a dramatic shift in the Red indices. LOSS data for Secondary activated-sludge systems were analyzed for a period of 60 days at the Blue Plains Advanced Wastewater Treatment Plant. LOSS numbers collected experimentally were validated with the Takacs et al. (1991) settling model. When compared to flux curves with small changes in the sludge concentration matrix, LOSS was found to be faster at characterizing the hindered settling velocity and was less erratic. Simple batch experiments based on the critical settling velocity (CSV) selection were used as the basis for the development of two novel parameters: threshold of flocculation/flocculation limitation (TOF/a), and floc strength. TOF quantified the minimum solids concentration needed to form large flocs and was directly linked to collision efficiency. In hybrid systems, an exponential fitting on a CSV matrix was proposed to quantify the collision efficiency of flocs (a). Shear studies were conducted to quantify floc strength. The methods were applied to a wide spectrum of sludge types to show the broad applicability and sensitivity of the novel methods. Three different activated sludge systems from the Blue Plains AWWTP were monitored for a 1 year period to explore the relationship between effluent suspended solids (ESS) and activated sludge settling and flocculation behavior. Novel metrics based on the transitional solids concentration (TOF, and LOSS) were also collected weekly. A pilot clarifier and settling column were run and filmed to determine floc morphological properties. SVI was found to lose sensitivity (r < 0.20) when characterizing ISV above a hindered settling rate of 3 m h-1. ISV and LOSS had a strong correlation (r = 0.71), but ISV was subject to change, depending on the solids concentration. Two sludge matrix limitations influencing ESS were characterized by transition concentrations; pinpoint floc formation, and loose floc formation. Pinpoint flocs had TOF values above 400 mg TSS L-1; loose floc formation sludge had TOF and LOSS values below 400 mg TSS L-1 and 900 mg TSS L-1, respectively. TOF was found to correlate with the particle size distribution while LOSS correlated to the settling velocity distribution. The use of both TOF and LOSS is a quick and effective way to characterize limitations affecting ESS. / Ph. D. / New parameters to determine how particles separate from water were developed. The new parameters didn’t require expensive or specialized instruments. Current parameters that don’t rely on specialized instruments give little information on how particles separate from water. The new parameters provide information on particle size, particle settling speed, and particle stickiness. The significance of this research is the ability for anyone in the field to gain a better understanding of the settling process they are monitoring using these parameters. The first parameter named Intrinsic Settling Classes allows one to determine the particle size distribution within a sludge mixture based on how fast the particles settle. The parameter requires dilution of the sample to inhibit the particles from sticking together. Bigger particles will settle faster, due to more mass assuming density does not change significantly. The second parameter named Limit of Stokesian Settling determines a particle’s settling speed. The parameter involved finding the maximum particles that could occupy a particular space before the particle-particle interaction start to hinder settling. Particles with a faster settling distribution can have more particles occupying a certain space before hindering one another. The third parameter named Threshold of Flocculation identified a particle’s stickiness. Stickier particles attach together in a shorter time resulting in large clumps of particles that settle faster. This parameter involved determining the minimum amount of particles needed to form large clumps of particles at a predetermined time. Stickier particles require less particles and time to form large clumps of particles.

Ett avgörande steg i avloppsvattenrening med aktivslammetoden är att slammet kan avskiljas från det renade vattnet. Detta sker oftast i sedimenteringsbassänger. Sedimenteringsproblem är dock vanliga på många avloppsreningsverk, vilket kan leda till att föroreningar följer med utgående vatten. Studier om slamegenskaper är därför viktiga för att öka förståelsen för vilka faktorer som påverkar sedimenteringen och vad som kan göras för att minska problemen. I detta examensarbete har ett mindre avloppsreningsverk i Lövsta utanför Uppsala studerats. Ungefär en tredjedel av flödet till verket kommer från ett närliggande slakteri. Verket har återkommande problem med flytslam i slutsedimenteringsbassängen. Syftet med arbetet var att – genom litteraturstudier, provtagning, fullskaleförsök och analys – identifiera orsaker till flytslamproblemen. Även förslag till åtgärder skulle läggas fram. En utvärdering av reningsprocessen genomfördes dels utifrån befintliga mätdata, dels genom utförligare provtagningar och mätningar som gjordes under en treveckorsperiod i oktober. Höga nitrathalter observerades i vattnet, särskilt i den utjämningstank där flödesutjämning och förbehandling av slakterivattnet sker. De höga nitrathalterna, i kombination med en lång slamuppehållstid i slutsedimenteringsbassängen, bedömdes vara orsaken till flytslambildningen eftersom båda dessa faktorer gynnar uppkomsten av kvävgas genom denitrifikation. Det är denna gas som lyfter slammet från botten av bassängen till ytan. Två möjliga åtgärder för att lösa flytslamproblemen bedömdes vara genomförbara. Den ena var att öka bortpumpningen av slam från botten av slutsedimenteringsbassängen, för att förkorta slamuppehållstiden. Den andra var att denitrifiera vattnet innan det når slutsedimenteringen. Av dessa förslag ansågs det senare vara mer tilltalande ur både ekonomisk och miljömässig synvinkel. En strategi för att denitrifiera vattnet bedömdes vara intermittent luftning av utjämningstanken. Denna luftningsstrategi tillämpades under en försöksperiod på tio veckor. Nitrathalterna mättes en eller två gånger i veckan under försöksperioden. En sänkning av nitrathalterna observerades: från 182 mg/l till som lägst 60 mg/l i utjämningstanken och från 65 mg/l till som lägst 18 mg/l i luftningsbassängen. Sänkningen var dock inte tillräcklig för att förhindra flytslambildning i sedimenteringsbassängerna. Den ofullständiga denitrifikationen berodde troligtvis på för låga BOD-halter i utjämningstanken. Vidare undersökningar behövs för att utvärdera och optimera kvävereningen genom intermittent luftning. / One of the most crucial steps in wastewater treatment with activated sludge is the separation of sludge from the treated effluent. This is most often done by sedimentation in clarifiers. Sedimentation problems are, however, common in many wastewater treatment plants, causing higher concentrations of pollutants in the effluent. Research on sludge characteristics is therefore important to increase the knowledge about which factors affect the sludge settling properties and what can be done to minimize problems. In this master thesis a small wastewater treatment plant in Lövsta outside Uppsala, Sweden, was investigated. About a third of the influent water to the plant comes from a slaughterhouse situated nearby. The plant suffers from recurrent problems with rising sludge in the secondary clarifier. The purpose of this thesis was to – through literature studies, sampling, full scale experiments and analysis – identify causes for the rising sludge problem. Also, suggestions to remedy the problem were to be presented. An evaluation of the treatment process was conducted from existing measurement data and from sampling and measuring done for three weeks in October. High concentrations of nitrate were found in the water, especially in the equalization tank, where flow equalization and primary treatment of the slaughterhouse wastewater takes place. The high concentrations of nitrate, in combination with a long sludge retention time in the secondary clarifier, were judged to be the reason for the formation of rising sludge. Both of these factors stimulate the development of nitrogen gas through denitrification. This gas lifts the sludge from the bottom of the clarifier to the water surface. Two possible solutions to the rising sludge problem were considered feasible. One was to increase the pumping of sludge from the bottom of the secondary clarifier, to decrease the sludge retention time. The second was to denitrify the water before it reaches the clarifiers. Of these two suggestions, the second was regarded as more appealing from both an economical and an environmental point of view. One method to denitrify the water was deemed to be intermittent aeration in the equalization tank. This aeration strategy was implemented for ten weeks. Nitrate concentrations were measured one or two times a week for this period of time. A decrease in nitrate concentrations was observed: from 182 mg/L to at its lowest 62 mg/L in the equalization tank, and from 65 mg/L to at its lowest 18 mg/L in the aeration tank. The decrease was, however, not enough to prevent formation of rising sludge in the clarifiers. The incomplete denitrification process was thought to be due to low concentrations of BOD in the equalization tank. Further studies need to be carried out to evaluate and optimize the nitrogen removal through intermittent aeration.