Manure contains nutrients for crop growth; however, overapplication, with time, can result in excess nutrients in soil, which can subsequently be lost in surface runoff. <p>The general purpose of this research is to study the effect of liquid hog manure, applied as an agricultural fertilizer, on water chemistry of surface runoff from rainfall simulation tests. Specifically the research focuses on runoff water chemistry comparisons between lands receiving hog manure at different rates, via different injection methods, and upon different slope positions. <p>To examine these objectives, soil nutrient supply rates (P, NH4-N, and NO3-N) of the 0 5 cm depth of soil adjacent to rainfall simulation positions, and runoff water chemistry (TP, OP, NH4-N, NO3-N, DOC, Cl- and coliforms) during rainfall simulation tests were collected before and after manure addition. <p> Generally, manure application did increase soil NH4-N and NO3-N supply rates, and runoff NH4-N concentration. Soil P supply rate and runoff TP concentration were not affected by the manure addition; however, runoff OP concentration at one site (Perdue) increased significantly due to manure addition. The manure treatments applied in this study did not cause any significant increases in fecal or total coliform in runoff from rainfall simulation tests conducted 7 8 months after manure application. None of the water quality parameters exceeded the Guidelines for Canadian Drinking Water Quality. <p> Manure injection method (regular versus low soil surface disturbance) had consistent effects on runoff chemistry, but application rate did not. The regular disturbance method had significantly higher concentrations of water quality parameters than the low disturbance method. <p> The position of the test on the slope did not result in any consistent trends in runoff chemistry, whether before or after manure addition. Foot slope positions had higher soil NH4-N supply rates than upper slope positions, both before and after manure addition. Soil NH4-N, NO3-N, and P supply rates between landscape positions were not likely influenced by manure addition. <p> Regression tests between soil nutrient supply rates and runoff chemistry indicate that soil NH4-N supply rates are a good index to predict runoff NH4-N concentration, but soil P did not predict runoff P.

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Orientador: Edson Lazarini / Banca: Marco Eustáquio de Sá / Banca: Edson Luiz Mendes Coutinho / Resumo: A busca pela qualidade do solo como base de sustentação do sistema de produção, tem aumentado o desafio de compreender um sistema que, além de reduzir sensivelmente a degradação do meio ambiente, pode permitir maior retorno econômico ao agricultor. Nesse sentido o presente trabalho foi desenvolvido no período de abril de 2005 a abril de 2006, com o objetivo de avaliar na cultura da soja, no sistema de plantio direto, o efeito de diferentes culturas de cobertura e presença ou ausência de adubação orgânica e mineral. O trabalho foi desenvolvido no município de Guararapes-SP (50°39'W, 21°09'S e 412 m de altitude aproximadamente) em um Latossolo Vermelho amarelo, com textura média, sendo cultivado com culturas anuais a partir de 1993. O delineamento experimental utilizado foi o em blocos casualizados com 4 repetições, com os tratamentos dispostos em um esquema fatorial 5x2x2, ou seja, 5 culturas de cobertura (milho, milheto, sorgo, braquiária e mamona), cultivadas no período de outono/inverno, na presença e ausência de adubação orgânica (11.512 kg/ha de esterco de galinha), sendo semeado em sucessão, a cultura da soja, cultivar MGBR 46 (Conquista), na presença ou ausência de adubação mineral (500 kg/ha da fórmula 02-20-15). As culturas de cobertura e a soja foram irrigadas pelo sistema pivô central. As avaliações constaram da produção de massa seca das culturas de cobertura, estado nutricional da soja no florescimento pleno, características agronômicas e produção de grãos da soja e características químicas do solo, em diferentes profundidades, após o cultivo da soja. Através dos resultados obtidos, verificou-se que o milho apresentou maior produção de massa seca como cultura de cobertura, mas todas as ...(Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The search for the soil quality as sustentation base of production system, has stimulated the challenge to understand a system that, beyond sensibly reducing the environment degradation, may allows greater economic return to the farmer. Thus, the present work was developed in the period of April 2005 to April 2006, with the objective to evaluate in soybean crop, in no tillage system, the effect of different cover crops and presence or absence of organic and mineral fertilization. The work was developed at the municipal district of Guararapes, State of São Paulo (50°39' W, 21°09' S and 412 m altitude approximately) in a Typic Haplustox, with sandy texture, being cultivated with annual crops since 1993. The exeprimental design used was randomized blocks with four replications, with treatments disposed in a 5x2x2 factorial scheme, i.e., five cover crops (corn, millet, sorghum, braquiaria grass and castor bean), cultivated in the autumn/winter period, in presence and absence of organic fertilization (11,512 kg ha-1 of chicken manure fertilizer), being sowedin succession of soybean crop, MGBR 46 cultivar, in presence or absence of mineral fertilization (500 kg ha-1 of 02-20-15 formula). The cover cultures and soybean had been irrigated by center pivot irrigation systems.The evaluations was cover crops dry matter production, nutritional status in flowering, agronomic characteristics and soybean grain production, soil chemical characteristis, in different depths, after soybean. Through the obtained results, it verified that the corn presents greater dry matter production as cover crop, but all the evaluated cover crops are adjusted to precede soybean; organic fertilization increases cover crop dry matter production, beyond provided significant P, Ca and K increases in 0 to 0.05 and 0.05 to 0.10 m depth and P and K in 0.10 to 0.20 m depth; ...(Complete abstract click electronic access below) / Mestre

Der Beitrag der arbuskulären Mykorrhizapilze zur Nährstoffaufnahme und zum Wachstum von Pflanzen ist vom Genotyp des Pilzes und der Pflanze abhängig, sowie von den Umweltbedingungen. In der vorliegenden Arbeit wurden Mykorrhizapilze unterschiedlicher Herkunft verwendet. Im Mittelpunkt der Arbeit stand die Untersuchung der Rolle der Mykorrhiza bei der Reaktion der Pflanze auf räumlich unterschiedliches Nährstoffangebot im Boden. Als Versuchspflanzen wurden Süßkartoffel und Tagetes verwendet. Für die Arbeit wurden verschiedene Modellexperimente durchgeführt. In speziell für diese Arbeit konstruierten Gefäßen wurden nicht-mykorrhizierte und mykorrhizierte Süßkartoffelpflanzen mit organischer Substanz versorgt, die entweder gleichmäßig oder heterogen im Substrat verteilt war. In weiteren Experimenten wurde mit Hilfe von "split-root" Systemen die Wirkung arbuskulärer Mykorrhizapilze auf ein lokales Angebot von mineralischem Phosphor und Stickstoff im Boden untersucht. Darüber hinaus wurde in Versuchen Kompost räumlich konzentriert im Substrat angeboten. Die Messungen umfassten den Mykorrhizierungsgrad der Wurzel, die Entwicklung des extraradikalen Myzels, die Trockenmasse der Pflanze sowie die Konzentrationen an Phosphor und Stickstoff in der Pflanze. Eine Besiedlung der Wurzeln mit arbuskulären Mykorrhizapilzen führte in den meisten Versuchsansätzenzu einer erhöhten Nährstoffaufnahme der Pflanze und zu einem verbesserten Wachstum. Ein besonders starkes Hyphenwachstum in Bodenzonen mit viel organischer Substanz wurde jedoch nicht beobachtet. Zugabe von Kompost führte teilweise zu einem Rückgang des Mykorrhizierungsgrades. Die Verwendung von organischem Material oder Kompost im Gartenbau kann sinnvoll sein und zur Verminderung von Mineraldüngung beitragen. Optimales Pflanzenwachstum und Mykorrhizawirkung erfordern jedoch eine gute Balance zwischen Art und Menge des organischen Stoffes bzw. Komposts, den Substrateigenschaften und den Pflanzen- und Pilzgenotypen. / The actual contribution of arbuscular mycorrhizal (AM) fungi to plant nutrient uptake depends on the fungal and plant genomes, and on environmental conditions. In the present study, AM fungi of different origin, for example isolated from plots with different long-term fertilizer application history, were used to quantify their contribution to plant nutrient uptake under situations of spatially heterogeneous soil nutrient distribution. Test plants for this study were sweet potato and marigold. Several model experiments were carried out. In specifically constructed growth containers, non-mycorrhizal and mycorrhizal sweet potato plants were supplied with organic matter either homogeneously or heterogeneously distributed in the substrate. Bacteria from a long-term organically fertilized soil were also added as a treatment. In other experiments using a split-root approach, the influence of AM fungi on the plant response to localized mineral phosphorus and nitrogen supply was studied. In a further experiment, the effects of localized compost supply on marigold plants inoculated with Glomus mosseae were investigated. Arbuscular mycorrhizal fungi increased nutrient uptake and growth of plants under most conditions, also when nutrients were heterogeneously distributed in soil. However, there was no indication of increased hyphal proliferation or activity in soil spots with high organic matter. Plant phosphorus status regulated the extent of AM root colonization. The extent of AM root colonization was partly decreased by application of organic matter and of compost to the substrate. Application of organic matter and/or compost can be beneficial in horticulture and can replace mineral fertilizer use. However, optimum plant growth and mycorrhizal function require a good balance between type and amount of organic matter or compost, growth substrate properties and plant and AM fungal genotype.

Der Mangel an Nährstoffen im Boden, hauptsächlich an Phosphor (P) und Stickstoff (N), verbunden mit einem hohen Salzgehalt und der generellen Verarmung landwirtschaftlicher Böden , sind ein ernstes Problem für die landwirtschaftliche Produktion weltweit. Daher besteht ein dringender Bedarf an Forschung und Entwicklung geeigneter landwirtschaftlicher Praktiken, um ungünstige Bodenbedingungen zu verringern und wenn möglich die Fruchtbarkeit von Kulturland wiederherzustellen. Die Verwendung von Rhizobakterien, die das Pflanzenwachstum (PGPR) fördern, kann sich bei der Entwicklung von Strategien zur Erleichterung des Pflanzenwachstums unter normalen Wachstumsbedingungen sowie unter abiotischen Stress als nützlich erweisen. Diese Bakterien bieten ihren pflanzlichen Wirten Vorteile, indem sie die Aufnahme von Bodenmineralien fördern und Pflanzen vor schädlichen Umwelteinflüssen schützen. Die vorliegende Arbeit bewertet die Rolle von in Kamerun natürlich vorkommenden PGPR an Mais und untersucht deren Potenzial als Bioimpfstoffe zur Steigerung des Pflanzenwachstums in Kamerun. Wir prüfen die Hypothese, dass einheimische Bakteriengemeinschaften aus Kamerun einen hohen Anteil an Bakterien aufweisen, deren Eigenschaften Kulturpflanzen helfen, mit ungünstigen Bedingungen umzugehen. In der vorliegenden Arbeit wurden dazu Bakteriengemeinschaften der Rhizosphäre von in Kamerun angebautem Mais isoliert und untersucht. Zum ersten Mal erfolgte eine umfassende phylogenetische Zuordnung aller kultivierbaren Bakterien, auf Grundlage ihrer potenziellen Fähigkeiten zur Förderung des Pflanzenwachstums. / Nutrient deficiencies in soil, mainly in phosphorus (P) and nitrogen (N), coupled to salinity and the impoverishment of agricultural soils, are a severe problem for agricultural production worldwide. Therefore, there is an urgent need for research and development of more suitable agricultural practices in order to reduce unfavorable conditions, and if possible, to restore the fertility of cultivated lands. The use of rhizobacteria, which promote plant growth (PGPR), can prove useful in developing strategies to facilitate plant growth under normal as well as under abiotic stress conditions. These bacteria offer benefits to plant hosts by promoting the uptake of soil minerals and protecting plants from environmental stresses. The thesis evaluates the role of native PGPR associated with maize as potential bio-inoculants for plants growth in Cameroon. We hypothesized that native bacterial communities from Cameroon include a high potential of bacteria helping the plant cope with unfavorable conditions. Here, we provide for the first time a comprehensive phylogenetic affiliation of cultivable bacterial communities associated with maize rhizosphere grown in Cameroon in relationship to their potential plant growth-promoting abilities.