<p>Органска ђубрива садрже готово све неопходне хранљиве елементе за гајене биљке, али имају значајно мању ефикасност од синтетичких ђубрива јер је садржај хранива неупоредиво нижи у односу на синтетичка, а облици у којима се налазе нису директно приступачни гајеним биљкама. Циљ истраживања био је да се испита утицај примене различитих органских ђубрива биљног и животињског порекла, као и течних ђубрива на хемијска својства земљишта, принос и квалитет јагоде у оквиру два система производње (малчирања земљишта). Пољски оглед је постављен као двофакторијални по методи/плану подељених парцела (Split/plot), са случајним распоредом третмана. Оглед се састојао од две главне парцеле на којима је испитан утицај два различита начина покривања земљишта (малч од сламе и црна полиетиленска фолија), док је на основним парцелама испитан утицај 13 различитих третмана ђубрења (контрола &ndash; без примене ђубрива, &Oslash;; говеђи стајњак, С; говеђи стајњак + гуано (фолијарно), С+Г; говеђи стајњак + екстакт глистењака (фолијарно), С+ЕГ; глистењак, ГЛ; глистењак + гуано (фолијарно), ГЛ+Г; глистењак + екстракт глистењака (фолијарно), ГЛ+ЕГ; компост из производње печурака, К; компост из производње печурака + гуано (фолијарно), К+Г; компост из производње печурака + екстракт глистењака, К+ЕГ; гуано (фертигација + фолијарно), Г; екстракт глистењака (фертигација + фолијарно), ЕГ; стандардни програм ђубрења са минералним NPK ђубривима, NPK). У узорцима земљишта одређен је садржај минералних облика N, приступачних облика P, K, Ca, Mg, Fe, Mn, Cu, Zn и pH вредност земљишта. Поред параметара приноса јагоде (број цветова, прој плодова, просечна маса плода) у све три године плодоношења одређени су садржај макро и микроелемената у листу и плоду јагоде, и параметри квалитета плода јагоде (укупан садржај растворљиве суве материје, укупан садржај киселина, садржај укупних антоцијана, антиоксидативна активност, обојеност и чврстина плода). Резултати истраживања су показали да се продужено дејство примене чврстих и течних органских ђубрива на садржај хранљивих елемената у земљишту значајно разликује у зависности од елемента који се посматра. Примена чврстих органских ђубрива имала је највећи утицај на садржај приступачних облика фосфора, затим калијума, док је најмањи утицај имала на садржај минералних облика азота у земљишту. Изузев стајњака, примена органских ђубрива, у количини еквивалентној 170 kg N ha-1, није имала утицај на концентрацију приступачних облика секундарних макроелемената и микроелемената у земљишту. Земљиште покривено црном ПЕ фолијом одликовало се вишим садржајем минералних облика азота и приступачних облика Fe, Mn, Zn и Cu у односу на земљиште покривено сламом, у свим терминима мерења. Примена чврстих органских ђубрива приликом садње јагоде довела је до значајног повећања приноса, у односу на контролу, само у првој години плодоношења, док је у другој и трећој години плодоношења највиши принос јагоде остварен на третманима где су примењена NPK ђубрива и течни гуано. Третмани ђубрења имали су значајан утицај на минерални састав, садржај растворљиве суве материје, садржај киселина, садржај антоцијана, антиоксидативну активност и обојеност плода. Примена црне полиетиленске фолије, поред позитивног утицаја на садржај хранљивих елемената у земљишту, довела је до вишег приноса и квалитета плода јагоде у односу на примену сламе као малча. Rезултати показују да се применом црне фолије може значајно повећати ефикасност ђубрива чиме се смањује ризик од загађења животне средине и остварује већа економска добит у производњи јагоде.</p> / <p>Organska đubriva sadrže gotovo sve neophodne hranljive elemente za gajene biljke, ali imaju značajno manju efikasnost od sintetičkih đubriva jer je sadržaj hraniva neuporedivo niži u odnosu na sintetička, a oblici u kojima se nalaze nisu direktno pristupačni gajenim biljkama. Cilj istraživanja bio je da se ispita uticaj primene različitih organskih đubriva biljnog i životinjskog porekla, kao i tečnih đubriva na hemijska svojstva zemljišta, prinos i kvalitet jagode u okviru dva sistema proizvodnje (malčiranja zemljišta). Poljski ogled je postavljen kao dvofaktorijalni po metodi/planu podeljenih parcela (Split/plot), sa slučajnim rasporedom tretmana. Ogled se sastojao od dve glavne parcele na kojima je ispitan uticaj dva različita načina pokrivanja zemljišta (malč od slame i crna polietilenska folija), dok je na osnovnim parcelama ispitan uticaj 13 različitih tretmana đubrenja (kontrola &ndash; bez primene đubriva, &Oslash;; goveđi stajnjak, S; goveđi stajnjak + guano (folijarno), S+G; goveđi stajnjak + ekstakt glistenjaka (folijarno), S+EG; glistenjak, GL; glistenjak + guano (folijarno), GL+G; glistenjak + ekstrakt glistenjaka (folijarno), GL+EG; kompost iz proizvodnje pečuraka, K; kompost iz proizvodnje pečuraka + guano (folijarno), K+G; kompost iz proizvodnje pečuraka + ekstrakt glistenjaka, K+EG; guano (fertigacija + folijarno), G; ekstrakt glistenjaka (fertigacija + folijarno), EG; standardni program đubrenja sa mineralnim NPK đubrivima, NPK). U uzorcima zemljišta određen je sadržaj mineralnih oblika N, pristupačnih oblika P, K, Ca, Mg, Fe, Mn, Cu, Zn i pH vrednost zemljišta. Pored parametara prinosa jagode (broj cvetova, proj plodova, prosečna masa ploda) u sve tri godine plodonošenja određeni su sadržaj makro i mikroelemenata u listu i plodu jagode, i parametri kvaliteta ploda jagode (ukupan sadržaj rastvorljive suve materije, ukupan sadržaj kiselina, sadržaj ukupnih antocijana, antioksidativna aktivnost, obojenost i čvrstina ploda). Rezultati istraživanja su pokazali da se produženo dejstvo primene čvrstih i tečnih organskih đubriva na sadržaj hranljivih elemenata u zemljištu značajno razlikuje u zavisnosti od elementa koji se posmatra. Primena čvrstih organskih đubriva imala je najveći uticaj na sadržaj pristupačnih oblika fosfora, zatim kalijuma, dok je najmanji uticaj imala na sadržaj mineralnih oblika azota u zemljištu. Izuzev stajnjaka, primena organskih đubriva, u količini ekvivalentnoj 170 kg N ha-1, nije imala uticaj na koncentraciju pristupačnih oblika sekundarnih makroelemenata i mikroelemenata u zemljištu. Zemljište pokriveno crnom PE folijom odlikovalo se višim sadržajem mineralnih oblika azota i pristupačnih oblika Fe, Mn, Zn i Cu u odnosu na zemljište pokriveno slamom, u svim terminima merenja. Primena čvrstih organskih đubriva prilikom sadnje jagode dovela je do značajnog povećanja prinosa, u odnosu na kontrolu, samo u prvoj godini plodonošenja, dok je u drugoj i trećoj godini plodonošenja najviši prinos jagode ostvaren na tretmanima gde su primenjena NPK đubriva i tečni guano. Tretmani đubrenja imali su značajan uticaj na mineralni sastav, sadržaj rastvorljive suve materije, sadržaj kiselina, sadržaj antocijana, antioksidativnu aktivnost i obojenost ploda. Primena crne polietilenske folije, pored pozitivnog uticaja na sadržaj hranljivih elemenata u zemljištu, dovela je do višeg prinosa i kvaliteta ploda jagode u odnosu na primenu slame kao malča. Rezultati pokazuju da se primenom crne folije može značajno povećati efikasnost đubriva čime se smanjuje rizik od zagađenja životne sredine i ostvaruje veća ekonomska dobit u proizvodnji jagode.</p> / <p>Organic fertilizers contain almost all the necessary nutritional elements for cultivated plants, but they have a significantly lower efficiency compared to synthetic fertilizers due to lower nutrient content and the forms of nutrients in which they are not directly accessible to the cultivated plants. The aim of this study was to quantify the effect of the application of different plant and animal origin organic fertilizer, as well as liquid fertilizers, on soil chemical properties, yield and quality of strawberries in two production systems (soil mulching). The field experiment was conducted using a 2-factorial split-plot completely randomized design, with ground cover management (mulch) as main-plot factor and fertilization treatments in sub-plot. On the main plots we examined the influence of two different mulches (straw and black polyethylene sheet), while on sub-plots the effect of 13 different treatments of fertilization was examined (control - no fertilization, &Oslash;; dairy manure, С; dairy manure + guano (foliar application), С+Г; dairy manure + vermicompost leachate (foliar application), С+ЕГ; vermicompost, ГЛ; vermicompost leachate + guano (foliar application), ГЛ+Г; vermicompost + vermicompost leachate (foliar application), ГЛ+ЕГ; spent mushroom compost, К; spent mushroom compost + guano (foliar application), К+Г; spent mushroom compost + vermicompost leachate, К+ЕГ; guano (fertigation + foliar application), Г; vermicompost leachate (fertigation + foliar application), ЕГ; standard fertilization with mineral NPK fertilizers, NPK). The soil samples were analysed to determine the content of mineral forms of N, available forms of P, K, Ca, Mg, Fe, Mn, Cu, Zn and soil pH value. Beside parameters of strawberry yield (number of flowers, number of fruits, average fruit weight) in all three fruiting years we determined the content of macro- and microelements in the leaves and fruits, as well as fruit quality parameters (total soluble solids, total acidity, total anthocyanins, antioxidant activity, coloring and fruit firmness). The results showed that application of solid and liquid organic fertilizers had a different residual effect on concentration of macro- and micro elements in the soil. Application of solid organic fertilizer had the greatest influence on the content of available forms of phosphorus, than potassium, while the least impact had on the content of mineral forms of nitrogen in the soil. Except for dairy manure, the application of organic fertilizers, in amount equivalent to 170 kg N ha-1, had no effect on concentration of available forms of secondary macroelements and microelements in the soil. Soil covered with black polyethylene mulch had higher content of mineral forms of nitrogen and available forms of Fe, Mn, Zn and Cu, compared to soil covered with straw mulch, in all terms of measuring. The application of solid organic fertilizers led to a significant increase in yield compared to the control in the first fruiting year, while in the second and third fruiting years the highest yields were achieved on the treatments where NPK fertilizer and liquid guano were applied. Fertilization treatments had a significant effect on the mineral composition, content of soluble solids, titrible acidity, content of anthocyanins, antioxidant activity and color of the strawberry fruits. Black polyethylene mulch had positive impact on concentration of macro- and microelements in soil and led to higher yield and quality of strawberry fruits compared to straw mulch. The results shows that the black polyethylene mulch can significantly increase fertilizer efficiency, which reduce the risk of environmental pollution and may result in greater economic gain in strawberry production.</p>

The dairy food industry is continuously striving towards products with higher quality and longer shelf-life available to the customer at low prices. Arla Foods in Linköping, Sweden, is currently investigating the possibilities of changing the material in yogurt packaging containers by replacing the currently used carton with a different and cheaper alternative. A successful switch will give the company an economical advantage without affecting the sensory attributes (smell, taste, sight, and consistency), aroma profile or other important trademarks of the yogurt. This study is designed to examine and compare yogurt that has been stored in different packaging cartons, one coated with a single-layered low-density polyethylene (LDPE) and one coated with a currently used multi-layered ethylene-vinyl alcohol (EVOH). The study was based on the analysis and measurement of sensory attributes performed by experts, physical properties in laboratory and chemical composition in GC-FID/MS together with a discriminative test where a group of people would identify any difference between the yogurts. Together, these analyses would provide an explanation about any differences between the packaging materials by connecting physical, chemical and/or sensory characteristics. The collected results would give a better and more comprehensive picture than each analysis would do separately. The results from the study show that there is a difference between yogurts stored in LDPE-based containers and yogurts stored in EVOH-based containers and that the product was chemically affected, mainly by the level of oxygen in contact with the food. The overall assessment is that the largest difference was discovered in the taste.

Der Bericht fasst die Ergebnisse eines Forschungsprojektes zusammen, in dem Möglichkeiten zur Optimierung der maschinellen Unkrautregulierung untersucht wurden, um eine Reduzierung des Handarbeitsaufwands zu erreichen. Mit der erprobten Gerätekombination ist eine Senkung der Handarbeit gegenüber dem bisher in der Praxis üblichen Verfahren um 50 bis 60 Prozent möglich. In der optimalen Variante zur Unkrautregulierung wurde bei einem Ertrag von 98 dt/ha (2013) bzw. 108 dt/ha (2014) und einem kalkulierten Durchschnittserlös von 3,00 €/kg die Gewinnzone erreicht. Die Ergebnisse richten sich an Praktiker und Berater aus dem ökologischen und konventionellen Gartenbau.

No description available.

Restoration of Lithuanian independence, land reform, radically changed farming structure and strawberry growing potential has conditioned an increase of modern strawberry plantation. Irrigation is a rather important precondition for the commercial cultivation of fruit and berries in Lithuania. The new farm structure requires new, mainly small-scale irrigation systems. Strawberry water consumption and the soil water dynamics during strawberry growing period stated performing pilot research and additional yield which had been got because of the irrigation influence allows to measure optimal irrigation regime of the strawberry based on research. The aim of the work was to investigate dynamics of strawberry evapotranspiration in the Middle Lithuania and in the course of experiments, with respect to development peculiarities and productivity, to determine optimal irrigation regime for the strawberry grown in loamy soils. In the summary is presented strawberry evapotranspiration results maintaining optimal soil moisture conditions with irrigation; analysis of reference evapotranspiration methods; determination of their interaction degree and evaluation of the possibilities to introduce reference evapotranspiration models in calculation of the evapotranspiration of strawberries; quantitative evaluation of the influence of meteorological factors on the amount of the water consumed by strawberries and analysis of strawberry evapotranspiration calculation methods; evaluation of soil... [to full text]

This thesis presents the results of historical archaeological research at the Old Farm on Strawberry Hill in Albany, Western Australia. The site is an important colonial farm in Western Australia’s history; the location for the first farm in Western Australia (1827) and linked to many important individuals in the state’s colonial past. The site is owned and managed by the National Trust of Australia (W.A.) and is registered on both the West Australian, Heritage Council Register of Historical Places and the Australian Heritage Commission’s National Estate. Past historical and cultural biases had created an incomplete interpretation of this site that did not represent all social groups, including indentured servants, convict and Aboriginal labourers and women. The research has provided a holistic site interpretation that identified all social groups living and working on this site in the 1800s by analysing historical documents and archaeological excavated materials. The historical documentary record included both personal and official correspondence, diaries and drawings, as well as two valuable farm log books that documented the day to day events on the farm in the early to mid 1800s. The archaeological excavation was restricted to small area excavations in habitation areas still present on the site or in areas identified from 19th century surveyor maps. Both of these data sources were analysed to identify social and economic relationships, such as gender, status, class and ethnicity so that a comparison could be made between historical and archaeological data and a complementary interpretation created. The research was divided into three main periods of site occupation, firstly by convict gardeners during the government farm period from 1827 to 1832. The Spencer family period from 1833 to 1889, which is further defined by two phases, the six years from their arrival until Richard Spencer’s death in 1839 and the dispersal of the family and the property decline until it was sold in 1889. The third period of occupation by the Bird family was not discussed due to the discontinuation of a farming subsistence that distinguished it from a rural rather than an urban property. This study provides the current heritage managers with an updated interpretation of the site’s past and changing social and economic relationships on site and with the early town of Albany. It is hoped that this interpretation will be used to improve the site’s current representation and becomes the basis for a heritage conservation plan which not only recognises the importance of existing site structures, but also sub surface remains. This thesis also identifies a number of avenues for future research that will further enhance the site’s interpretation.

La présente étude concerne l’évaluation de l’impact du séchage par air chaud (HAD), lyophilisation (FD) et « swell drying » (SD), procédé couplant le séchage par air chaud avec le procédé de Détente Instantanée Contrôlée (DIC), sur les fraises (Fragaria var. Camarosa). Il s’agit de comparer et de contraster les performances des procédés et la qualité du produit fini séché en termes des cinétiques de séchage et de réhydratation, de contenus en molécules bioactives et activité antioxydante, et des paramètres caractéristiques de texture comme croquant et croustillant. Les résultats obtenus ont montré que le procédé de SD comparé aux procédés classiques de séchage et de lyophilisation, réduit d’une façon importante le temps de séchage ainsi que les coûts d’opération. D’autre part, SD conserve la qualité nutritionnelle des fraises en gardant leur contenu en composants bioactifs et en augmentant leur disponibilité. De plus, une corrélation importante entre la capacité antioxydante et le contenu total d’anthocyanes a été établie. D’autre part, les fraises séchées par SD ont montré une très intéressante macro et micro-structure. Les produits ont présenté une haute expansion et une croustillance significative due au phénomène de micro-alvéolation par décompression instantanée par DIC. D’ailleurs, il a été possible de mesurer les caractéristiques instrumentales de croustillance/croquance des échantillons finaux séchés. Grâce à la possibilité de modifier, contrôler et optimiser les paramètres opératoires du procédé DIC, il a été possible d’obtenir un produit du type « snack » croustillant avec une très haute valeur nutritionnelle. / The aim of this study was to evaluate the effect of hot air drying (HAD), freeze-drying (FD) and swell drying (SD), which is a coupling of hot air drying to instant controlled pressure drop, (DIC) on the strawberry (Fragaria var. Camarosa) to compare and to contrast its quality in terms of drying and rehydration kinetics, bioactive compounds and its antioxidant activity, and texture parameters as crunchy and crispy features. The obtained results shown that SD method helped to reduce the drying time leading to a low-cost processing compared with classical hot air drying and freezedrying. SD globally preserved the strawberry’s nutritional value and bioactive compounds, increasing their availability. Moreover, a strong correlation between antioxidant activity and total anthocyanin content was established in SD strawberries. On the other hand, the swell-dried strawberries showed an interesting macro and micro-structure. They presented a high expansion ratios and significant crispness provoked by the micro-alveolation phenomenon induced as consequence of the instant decompression process in the DIC treatment. Moreover, it was possible to instrumentaly measure the crispy/crunchy features of the final dried samples. By assessing such crispy and healthy contents of fruit “snacking”, it was possible to modify, control, and optimize DIC operating parameters. And, it can be designed according to the industrial or consumer needs.

Rhabdoviridae family viruses attacking the plant hosts were only described at the angiosperms. In this work, a gymnosperm rhabdoviridae infection was described for the first time ever ? specifically at Ginkgo biloba. Even though there were not observed any kinds of obvious infection symptoms on any of randomly chosen plant samples, through the molecular methods and detection primers the rhabdoviridae infection was proved at six of the plant samples. The acquired nucleotide and amino acid sequences, which were compared with the GenBank sequences, confirm the Rhabdoviridae family viruses occurrence. These given sequences demonstrated a certain analogy with a Strawberry crinkle virus assigned to the genus of Cytorhabdoviruses. The analyses proved a different reciprocal homology among the nucleotide sequences of the individual isolates, and in all likelihood an occurrence of two up to now unknown viruses in the Ginkgo biloba samples was proved for the first time. A definite categorization will be dependent on an acquisition and comparison of other sequences from the isolates genome and also on certain biological characteristics observation.

The thesis focuses on partial assessment of the life cycle of the strawberry plant (Fragaria), the breeding of which has a long tradition in the Czech Republic. The breeding technologies in conventional and ecological farming systems are compared, in relation to the greenhouse gases emission. The data from agriculture and transportation were accumulated by way of questionnaire survey and expert literature.The objective of this thesis was to assess the impact of individual agrotechnical factors on the pollution of the environment with greenhouse gases emissions in conventional and ecological farming systems, and also to test the hypothesis on whether it is better, from the environmental point of view, to consume locally produced or imported strawberries.The environmental impact was calculated with the SIMAPro software. This tool uses the Ecoinvent databases and enables to create a model of life cycle of the product in question according to the ČSN EN ISO 14040 and ČSN EN ISO 14044 standards. Theinputs and outputs were related to the unit of one hectare and the resulting value was converted as per 1 kg of strawberries. The input included technological operations, amount of fertilisers, and plant protection products; the output of the analysis was the emission load per1 kg of strawberries expressed in the CO2 equivalent.

Neozygites floridana is an important natural enemy of Tetranychus urticae in many crops, including strawberry. In this crop there has been some changes in the production system from open field to low tunnel. This new system presents benefits to strawberry production, moreover this system increases problems with spider mites and some fungal plant pathogens; consequently increasing the use of pesticides. In order to integrate N. floridana in the management of spider mites in strawberry there are still many important aspects to be revealed on the biology, epizootiology of the fungus and the effect of crop systems on its prevalence in the field. For this, three studies were performed, being the first one about the effect of strawberry production systems (low tunnel and open field) and pesticide application on population dynamics of two-spotted spider mite T. urticae, its natural enemies (N. floridana and predatory mites), and plant diseases, the experiment was performed in Inconfidentes-MG. The population of T. urticae attained the highest level in treatments with chemical control of pest, independently of the crop system, low tunnel or open field. The fungus N. floridana was observed late in both crop seasons, in treatments with and without inoculative releases, and has suggested that it might be a natural occurrence of the fungus. The highest prevalence of the fungus were observed in treatments with high densities of T. urticae. The predatory mites occurred naturally during the two crop seasons, and the most abundant species was Neoseiulus anonymus (Chant) (57.5%). The incidence of fungal plant diseases are greater in the open field than in low tunnel, however it seems that the crop system do not affect the new disease \"vermelhao\". The second study aimed to reveal the abiotic and biotic factors involved in the regulation of resting spores formation in the hosts T. urticae and T. evansi. The Brazilian isolate ESALQ1420 produced a large number of resting spores (51.5%) in T. urticae at a temperature 11 °C, photoperiod of 10L:14D, light intensity of 42-46 (?mol m-2s-1), on non-senescent plants. Small percentages of mites with resting spores (0-5 %) were found for the Norwegian isolate NCRI271/04 under the conditions tested, and very low percentages of resting spores (up to 1%) were observed in T. evansi infected by the Brazilian isolate ESALQ1421.The third study was therefore conducted phylogenetic analysis of isolates of N. floridana and develops efficient real-time PCR-based analysis for detection and quantification of N. floridana propagules from the soil. The phylogenic three showed that Neozygites is a fungal group far from other Entomophthoromycota and that N. floridana present high intra-specific variability. This data indicates that the isolates from different Tetranychus species may be in fact different Neozygites species, not described yet. We developed a real-time PCR probe for detection and quantification of N. floridana in the soil. This molecular probe was successful in quantifying N. floridana at relatively high DNA concentrations seeded into the soil. / Neozygites floridana é um importante inimigo natural de Tetranychus urticae em diversas culturas, incluindo morango. Nesta cultura tem havido uma mudança no sistema de produção, passando de cultivo em campo aberto para túnel baixo. Este novo sistema apresenta benefícios na produção de morango, no entanto pode aumentar problemas com ácaros fitófagos e algumas doenças fúngicas, consequentemente aumentando o uso de agrotóxicos. Para a integração de N. floridana no manejo de ácaros fitófagos em morango, existem ainda muitos aspectos importantes a serem conhecidos sobre a biologia, epizootiologia do fungo e efeito dos sistemas de cultivo sobre a sua prevalência em campo. Para isso três estudos foram realizados, sendo o primeiro estudo sobre o efeito de sistemas de produção de morango (túnel baixo e campo aberto) e da aplicação de agrotóxicos sobre a dinâmica populacional do acaro rajado T. urticae, seus inimigos naturais (N. floridana e ácaros predadores) e doenças de plantas, o experimento foi realizado em Inconfidentes-MG. A população de T. urticae atingiu altos níveis em tratamentos com controle químico de pragas, independente do sistema de cultivo, túnel baixo ou campo aberto. O fungo N. floridana foi observado no final de ambos os ciclos de cultivos, nos tratamentos com e sem liberações inoculativas, sugerindo que houve incidência natural do fungo. As maiores prevalências do fungo foram observadas em tratamentos com altas densidades de T. urticae. Os ácaros predadores ocorreram naturalmente durante os dois ciclos de cultivo, sendo Neoseiulus anonymus (Chant) a espécie mais abundante (57.51%). A incidência de doenças fúngicas foi maior em campo aberto do que em túnel baixo, no entanto parece que o sistema de cultivo não afetou a incidência da nova doença vermelhão. No segundo estudo objetivou-se conhecer os fatores abióticos e bióticos envolvidos na formação de esporos de resistência nos hospedeiros T. urticae e Tetranychus evansi. O isolado brasileiro ESALQ1420 produziu um grande número de esporos de resistência (51.5%) em T. urticae, a uma temperatura de 11 ° C, fotoperíodo de 10F:14E, intensidade de luz de 42-46 (?mol m-2s-1), em plantas senescentes. Pequenas percentagens de ácaros com esporos de resistência (0-5%) foram encontrados para o isolado Norueguês NCRI271/04 sob as condições testadas, e muito baixas percentagens de esporos de resistência (ate 1%) foram observados em T. evansi infectados pelo isolado brasileiro ESALQ1421. O terceiro estudo objetivou analisar filogeneticamente os isolados de Neozygites patogênicos a ácaros, e desenvolver ferramenta utilizando PCR em tempo real para a detecção e quantificação de propágulos de N. floridana a partir do solo. A arvore filogenética mostrou que Neozygites e um grupo de fungos distante dos demais Entomophthoromycota, e que estes fungos apresentaram alta variabilidade intraespecífica entre isolados de N. floridana. Estes resultados indicam que isolados provenientes de diferentes espécies hospedeiras do gênero Tetranychus podem ser de fato diferentes espécies de Neozygites não descritas ainda. Desenvolveu-se uma sonda molecular para detecção e quantificação de N. floridana no solo utilizando PCR em tempo real. Esta sonda foi eficiente na quantificação de N. floridana, em concentrações relativamente altas de DNA inoculado no solo.