The progress in information and communication technologies, together with scientific pro-gress in economics, statistics and operational research, influenced the rapid development of process understanding, and estimation and prediction of customer demand, in real-time revenue management. The aim of this work was the detailed description of the essential principles to be included in the business strategy in order to maximize revenue. Network revenue management models seek to maximize revenue when customers buy products with multiple sources. This concept was applied to the example of the aviation industry and a computing program was developed which could be generalized to other industries. This used a "hub-and-spoke" network and programs MS Excel, LINGO and VBA. The paper also describes the basic steps of revenue management that are repeated todetail the fore-casts and dynamically perform re-optimization of the required decisions, to specify the complete process in detail.

The in vitro cell culture models of human pluripotent stem cells (hPSC)-derived cardiomyocytes (CMs) have gained a predominant value in the field of drug discovery and is considered an attractive tool for cardiovascular disease modellings. However, despite several reports of different protocols for the hPSC-differentiation into CMs, the development of an efficient, controlled and reproducible 3D differentiation remains challenging. The main aim of this research study was to understand the changes in the gene expression as an impact of spatial orientation ofhPSC-derived CMs in 2D(two-dimensional) and 3D(three-dimensional) culture conditions and to identify the topologically important Hub and Hub-Bottleneck proteins using centrality measures to gain new knowledge for standardizing the pre-clinical models for the regeneration of CMs. The above-mentioned aim was achieved through an extensive bioinformatic analysis on the list of differentially expressed genes (DEGs) identified from RNA-sequencing (RNA-Seq). Functional annotation analysis of the DEGs from both 2D and 3D was performed using Cytoscape plug-in ClueGO. Followed by the topological analysis of the protein-protein interaction network (PPIN) using two centrality parameters; Degree and Betweeness in Cytoscape plug-in CenTiScaPe. The results obtained revealed that compared to 2D, DEGs in 3D are primarily associated with cell signalling suggesting the interaction between cells as an impact of the 3D microenvironment and topological analysis revealed 32 and 39 proteins as Hub and Hub-Bottleneck proteins, respectively in 3D indicating the possibility of utilizing those identified genes and their corresponding proteins as cardiac disease biomarkers in future by further research.

Neuartige Materialien spielen eine entscheidende Rolle in Innovationsprozessen und sind die Voraussetzung für eine Vielzahl neuer Produkte. Der Standort Dresden stellt mit der Exzellenz-Universität TU Dresden und einer Vielzahl an außeruniversitären Einrichtungen ein bedeutendes europäisches Zentrum auf dem Gebiet der Materialforschung dar. Das breite wissenschaftliche und technologische Spektrum sowie die enorme Forschungsdichte in Kombination mit einer hohen fachlichen Vernetzung führen einerseits zu Synergieeffekten unter den Wissenschaftlern und verschaffen andererseits der Wirtschaft einen enormen Standortvorteil. Sollen diese Vorteile voll ausgenutzt werden, bedarf es eines vereinheitlichten, intuitiven Informationszugangs. Aktuell werden Materialdaten jedoch typischerweise auf einer Vielzahl separierter, teilweise eingeschränkt zugänglicher Datenbestände gehalten und sind nach heterogenen Schemas und in variierendem Detailgrad beschrieben. Zwar existieren bereits Rechercheportale, diese sind jedoch domänenspezifisch, kostenpflichtig oder bieten nur auf spezielle Zielgruppen zugeschnittene Bedienoberflächen, die für andere Nutzer kaum bedienbar sind. Verteilte Recherchen über mehrere Datenquellen und Portale sind zeitaufwändig und mühsam. Abhilfe soll die hier vorgestellte integrierte Material-Recherche-Plattform Material Hub schaffen. Sie muss den Anforderungen von Herstellern und Zulieferern, deren Daten sie enthält ebenso entsprechen wie den Anforderungen der Anwender aus Forschung, Industrie und Handwerk. Diese den Wissenschaftsraum Dresden integrierende Plattform soll weitere erstklassige Forschungs- und Innovationsleistungen stimulieren, Kooperationen begünstigen und die Vermarktung innovativer Ideen und Lösungen wesentlich erleichtern. Außerdem soll Material Hub die Sichtbarkeit und Reichweite der Dresdner Materialforschung erhöhen und so die bereits vorhandene Leistungsfähigkeit signifikant stärken. Gegenstand dieses Artikels ist das technische Grundkonzept des Material Hub. Ein wesentlicher Aspekt besteht dabei in der Zusammenführung verschiedener Datenquellen in einem zentralen Rechercheportal. Integriert werden Forschungsdaten, Herstellerinformationen und Anwendungsbeispiele, die sowohl hinsichtlich Domäne als auch hinsichtlich Detailgrad und 1 gefördert aus Mitteln der Europäischen Union und des Europäischen Fonds für regionale Entwicklung zugrundeliegendem Schema heterogen sind. Dazu wird in Abstimmung mit Werkstoffwissenschaftlern ein Schema zur Materialbeschreibung sowie eine semantische Wissensbasis konzipiert, die z. B. Synonyme und inhaltliche Zusammenhänge modelliert. Basierend darauf werden die Datenbestände indexiert und für die Recherche zugänglich gemacht. Die Benutzeroberfläche unterstützt mehrere Suchmasken, von der klassischen Stichwortsuche über die facettierte Suche bis hin zu stärker geführten Ansätzen, um zielgruppenspezifischen Anwendungsfällen durch geeignete UI-Konzepte gerecht zu werden. Neben konzeptionellen Ansätzen behandelt dieser Artikel erste Implementierungs- und Evaluationsergebnisse.

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Fungi have a key role in the decomposition of coniferous litter and affect nutrient cycling in forest ecosystems. Therefore, great emphasis is placed on exploring the diversity of these organisms. The aim of this thesis was to describe fungal diversity in spruce litter and revealed temporal development of this community in a forest regenerating after bark beetle outbreak. Another objective was to compare sites with different length of bark beetle damage. The study area was located in the Bohemian Forest mountain range. Litter bags with spruce needles were placed on the forest soil and several samplings were performed in the course of three years. Diversity of fungi and changes in the fungal community were assessed by two methods: cultivation of needles on 2ř MEA and fingerprinting method T-RFLP. In total 71 fungal species were obtained from needles during a three-year succession by cultivation approach. Using T-RFLP 122 different fragments were generally recorded. The dominant species were Scleroconidioma sphagnicola, Thysanophora penicillioides, Hormonema dematiodes, Ceuthospora pinastri, species of genus Chalara, Trichoderma polysporum, Mycena galopus and unknown species Helotiales sp. 1. Primary saprotrophs occured in the community mainly in first 8 months and then were replaced by basidiomycetes....

Submitted by Adriana Alves Rodrigues (aalves@espm.br) on 2017-11-07T14:35:01Z No. of bitstreams: 1 MARCELO MAY SPINA JUNIOR.pdf: 1379956 bytes, checksum: d08b0255c146bc7b95ec89c6d23e1dc8 (MD5) / Approved for entry into archive by Adriana Alves Rodrigues (aalves@espm.br) on 2017-11-07T14:35:30Z (GMT) No. of bitstreams: 1 MARCELO MAY SPINA JUNIOR.pdf: 1379956 bytes, checksum: d08b0255c146bc7b95ec89c6d23e1dc8 (MD5) / Approved for entry into archive by Ana Cristina Ropero (ana@espm.br) on 2017-11-10T12:13:51Z (GMT) No. of bitstreams: 1 MARCELO MAY SPINA JUNIOR.pdf: 1379956 bytes, checksum: d08b0255c146bc7b95ec89c6d23e1dc8 (MD5) / Made available in DSpace on 2017-11-10T12:14:11Z (GMT). No. of bitstreams: 1 MARCELO MAY SPINA JUNIOR.pdf: 1379956 bytes, checksum: d08b0255c146bc7b95ec89c6d23e1dc8 (MD5) Previous issue date: 2017-03-22 / This research has as its theme the collaborative work and the entrepreneurial activity within the Impact Hub São Paulo, a coworking company whose mission is to provide a focused on entrepreneurial productivity through collaboration, exchange of experiences and networking among different projects. We aim to entrepreneurship (such as work, culture and style of life) and collaborative work, in addition to their meanings and relevance in the contemporary world - especially for members of Impact Hub São Paulo. To this end, research theoretical approach to French line discourse analysis (ORLANDI, 1999) for understand the symbolic production and ideology present in the Impact Hub communication. Subsequently, through the application of semi-structured interviews (BONI, QUARESMA, 2005), an analysis of the Hub's own speeches on the work collaborative and also the consumer relations that occur within the space itself. O theoretical framework that supports this empirical work is based on authors such as Boltanski and Chiapello (2009), Sennett (2005, 2006, 2009, 2012), Ehrenberg (2010), Han (2013, 2015) Gaulejac (2007), Casaqui (2014, 2015a, 2015b, 2016a, 2016b), among others. According to analysis of the results, we understand that Impact Hub São Paulo seeks to position itself as a space that promotes collaborative relationships among its members with the promise of that each project will present a better business performance and that, therefore, these projects will build a "better world" through social impact. However, from the analysis of discourses of its members, we understand that the working relationships within this space are not, in fact, collaborative relationships and that the call to build a "new world" "collaborative work of high impact" is merely a part of the rhetoric of capitalism in order to engage individuals in this mode of production. / Esta pesquisa tem como tema o trabalho colaborativo e a atividade empreendedora dentro do Impact Hub São Paulo, uma empresa de coworking que tem, como missão, proporcionar um espaço voltado à produtividade empreendedora por meio da colaboração, troca de experiências e formação de redes de trabalho entre diferentes projetos. Temos como objetivo compreender, em perspectiva crítica, o empreendedorismo (como trabalho, cultura e estilo de vida) e o trabalho colaborativo, além de seus significados e relevância na contemporaneidade – especialmente para os membros do Impact Hub São Paulo. Para tanto, a investigação utiliza como abordagem teórica a análise do discurso de linha francesa (ORLANDI, 1999) para compreender a produção simbólica e a ideologia presentes na comunicação do Impact Hub. Posteriormente, através da aplicação de entrevistas semi-estruturadas (BONI; QUARESMA, 2005), é feita a análise dos discursos dos próprios membros do Hub acerca do trabalho colaborativo e, também, das relações de consumo que ocorrem dentro do próprio espaço. O quadro teórico que dá suporte a esse trabalho empírico é baseado em autores como Boltanski e Chiapello (2009), Sennett (2005; 2006; 2009; 2012), Ehrenberg (2010), Han (2013; 2015) Gaulejac (2007), Casaqui (2014; 2015a; 2015b; 2016a; 2016b), entre outros. Conforme a análise dos resultados, compreendemos que o Impact Hub São Paulo busca posicionar-se como um espaço que promove relações colaborativas entre seus membros com a promessa de que cada projeto apresentará um melhor desempenho empresarial e que, assim, estes projetos irão construir “um mundo melhor” através do impacto social. Contudo, a partir da análise dos discursos de seus membros, compreendemos que as relações de trabalho dentro deste espaço não são, de fato, relações colaborativas e que a convocação para construir um “novo mundo” a partir de um “trabalho colaborativo de alto impacto” configura-se meramente como parte da retórica do capitalismo a fim de engajar os indivíduos neste modo de produção.

Anhand eines der erfolgreichsten Theaterstücke der Saison 2007/2008 untersucht der Beitrag die Repräsentation und Rolle von Schrift und Schreiben in Ulrich Hubs für Kinder verfassten "An der Arche um acht".

Univ., FB Wirtschaftswiss., Diss.--Hamburg, 2001. / Literaturverz. S. [193] - 208.

Cette thèse s’intéresse à l’exploitation de la sémantique de données pour la recherche exploratoire. La recherche exploratoire se réfère à des tâches de recherche qui sont très ouvertes, avec de multiples facettes, et itératives. Les données sémantiques et les données liées en particulier, offrent de nouvelles possibilités pour répondre à des requêtes de recherche et des besoins d’information complexes. Dans ce contexte, le nuage de données ouvertes liées (LOD) joue un rôle important en permettant des traitements de données avancés et des interactions innovantes. Nous détaillons un état de l’art de la recherche exploratoire sur les données liées. Puis nous proposons un algorithme de recherche exploratoire à base de données liées basé sur une recherche associative. A partir d’un algorithme de propagation d’activation nous proposons une nouvelle formule de diffusion optimisée pour les graphes typés. Nous proposons ensuite des formalisations supplémentaires de plusieurs modes d’interrogation avancée. Nous présentons également une architecture logicielle innovante basée sur deux choix de conception paradigmatiques. D’abord, les résultats doivent être calculés à la demande. Deuxièmement, les données sont consommées à distance à partir de services SPARQL distribués. Cela nous permet d’atteindre un niveau élevé de flexibilité en termes d’interrogation et de sélection des données. L’application Discovery Hub implémente ces résultats et les présente dans une interface optimisée pour l’exploration. Nous évaluons notre approche grâce à plusieurs campagnes avec des utilisateurs et nous ouvrons le débat sur de nouvelles façons d’évaluer les moteurs de recherche exploratoires. / The general topic of the thesis is web search. It focused on how to leverage the data semantics for exploratory search. Exploratory search refers to cognitive consuming search tasks that are open-ended, multi-faceted, and iterative like learning or topic investigation. Semantic data and linked data in particular offer new possibilities to solve complex search queries and information needs including exploratory search ones. In this context the linked open data cloud plays an important role by allowing advanced data processing and innovative interactions model elaboration. First, we detail a state-of-the-art review of linked data based exploratory search approaches and systems. Then we propose a linked data based exploratory search solution which is mainly based on an associative retrieval algorithm. We started from a spreading activation algorithm and proposed new diffusion formula optimized for typed graph. Starting from this formalization we proposed additional formalizations of several advanced querying modes in order to solve complex exploratory search needs. We also propose an innovative software architecture based on two paradigmatic design choices. First the results have to be computed at query-time. Second the data are consumed remotely from distant SPARQL endpoints. This allows us to reach a high level of flexibility in terms of querying and data selection. We specified, designed and evaluated the Discovery Hub web application that retrieves the results and present them in an interface optimized for exploration. We evaluate our approach thanks to several human evaluations and we open the discussion about new ways to evaluate exploratory search engines.

Orientador: Jorg Kobarg / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-18T17:04:45Z (GMT). No. of bitstreams: 1 Meirelles_GabrielaVaz_D.pdf: 11269390 bytes, checksum: bfd22a079ffc68a78b96b3e50dd60b1c (MD5) Previous issue date: 2011 / Resumo: A proteína NIMA foi identificada e caracterizada funcionalmente em Aspergillus nidulans como sendo uma serina/treonina cinase critica para a progressão do ciclo celular. As Neks (NIMA-related kinases) constituem uma família de cinases composta por 11 membros em mamíferos, que compartilham 40-45% de identidade com a proteína NIMA no domínio catalítico N-terminal. As Neks estão associadas a funções do ciclo celular e diversas patologias, o que as torna potenciais alvos quimioterápicos. Mutações no gene da Nek1 levam ao desenvolvimento da doença renal policistica e ao aparecimento de diversos efeitos pleiotrópicos, sugerindo sua participação em vias reguladoras de vários processos celulares. A Nek6, por sua vez, e ativada durante a mitose, e a super-expressão de mutantes inativos ou a sua depleção por RNAi produz células exibindo defeitos no fuso, anormalidades nucleares, parada na metáfase e apoptose. A Nek6 humana foi recentemente associada a carcinogênese, mas, assim como para a maioria das Neks, sua estrutura molecular, parceiros de interação e vias de sinalização permanecem ainda desconhecidos. Nesse trabalho, introduzimos a hNek6 como uma hub no interactoma humano. Uma extensa comparação de bancos de dados baseada em analises de conectividade mostrou que o quinoma humano e enriquecido em hubs. Nossas redes de interação incluem um amplo espectro de novos parceiros de interação para a hNek6 identificados em screenings de duplo - hibrido em levedura, classificados em 18 categorias funcionais. Alguns novos parceiros de interação da hNek6 são também possíveis substratos e, ainda, colocalizam com a hNek6 e ?-tubulina em células humanas, apontando para uma possível interação centrossomal. Os diversos parceiros de interação conectam a hNek6 a novas vias, como a sinalização de Notch e a regulação do citoesqueleto de actina, ou fornecem novas pistas de como a hNek6 poderia regular vias previamente propostas, como ciclo celular, reparo de DNA e sinalização do NF-?B. Alem disso, obtivemos o primeiro modelo estrutural de baixa resolução para a hNek6 a partir de SAXS. Analises estruturais revelaram que a hNek6 e um monômero em solução, apresentando uma conformação predominantemente globular, mas levemente alongada. Particularmente, a curta região N-terminal desordenada da hNek6 e importante para mediar as interações com seus parceiros. No caso da hNek1, observamos que ela interage com Fez1 e Clasp2 através de seus motivos coiled-coil, e colocaliza com essas proteínas em uma região candidata ao centrossomo / Abstract: NIMA was identified and functionally characterized in Aspergillus nidulans as a critical Ser/Thr kinase for cell cycle progression. The mammalian Neks (NIMA-related kinases) represent an evolutionarily conserved family of 11 serine/threonine kinases that share 40-45% identity with NIMA N-terminal domain. Neks are associated to cell cyclerelated functions and diverse pathologies, which highlight them as potential chemotherapeutic targets. Nek1 gene mutations lead to the development of polycystic kidney disease and the emergence of several pleiotropic effects, suggesting its involvement in pathways regulating various cellular processes. Nek6, in turn, is activated during mitosis, and overexpression of inactive mutants or its depletion by iRNA produces cells exhibiting mitotic spindle defects, nuclear abnormalities, metaphase arrest and apoptosis. Human Nek6 was recently found to be linked to carcinogenesis, but as for the majority of Neks, the molecular structure, interacting partners and signaling pathways remain elusive. Here we introduce hNek6 as a hub kinase in the human interactome. We performed a broad databank comparison based on degree distribution analysis and found that the human kinome is enriched in hubs. Our networks include a large set of novel hNek6 interactors identified in our yeast two-hybrid screens, classified into 18 functional categories. Some novel interactors are also putative substrates and colocalized with hNek6 and ?-tubulin in human cells, pointing to a possible centrosomal interaction. The interacting proteins link hNek6 to novel pathways, e.g. Notch signaling and actin cytoskeleton regulation, or give new insights on how hNek6 may regulate previously proposed pathways such as cell cycle, DNA repair and NF-?B signalings. Furthermore, we obtained the first low-resolution structural model of hNek6 by SAXS. Structural analysis revealed that hNek6 is a monomer in solution with a mostly globular, though slightly elongated conformation. Notably, we found that hNek6 unfolded short N-terminal region is important to mediate the interactions with its partners. In the case of hNek1, we found that it interacts with Fez1 and Clasp2 through coiled-coil motifs and colocalizes with these proteins in a candidate centrosomal region / Doutorado / Bioquimica / Doutor em Biologia Funcional e Molecular