Made available in DSpace on 2014-06-11T19:35:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-09-22Bitstream added on 2014-06-13T19:24:47Z : No. of bitstreams: 1 cavichioli_jc_dr_ilha.pdf: 512413 bytes, checksum: cd81d5957ea863badb365f4bca783ea8 (MD5) / A cultura do maracujazeiro-amarelo (Passiflora edulis f. flavicarpa Deg.) tem sofrido com várias doenças, o que tem comprometido severamente a produção, determinando a migração da cultura. Dentre as doenças, a morte prematura de plantas tem se destacado, causando prejuízos nas lavouras do Estado de São Paulo. Essa doença tem sido associada a fungos do solo, como Fusarium oxysporum f. passiflorae, Fusarium solani, Phytophthora sp. e também a bactéria, como Xanthomonas axonopodis f. passiflorae. As medidas de controle da morte prematura são preventivas, e, uma vez afetada pelos patógenos, a planta morrerá, pois não há controle curativo. A aplicação de defensivos químicos não tem sido eficiente na solução do problema da morte prematura de plantas. A enxertia do maracujazeiro-amarelo em portaenxerto resistente é uma técnica promissora para o controle desta doença. Esse trabalho teve como objetivo verificar o desempenho de plantas de maracujazeiro-amarelo (Passiflora edulis f. flavicarpa) enxertadas em três diferentes espécies de maracujazeiro, em dois sistemas de enxertia, em área com e sem histórico de morte prematura. Foram conduzidos três experimentos, sendo dois em viveiro e um no campo, no município de Adamantina, SP, na região da Nova Alta Paulista, no período de dezembro de 2005 a julho de 2007. Pelos resultados verificou-se que os dois métodos de enxertia utilizados foram bem sucedidos para as três espécies de maracujazeiros estudadas. A utilização de P. giberti e P. alata como portaenxerto para P. edulis Sims f. flavicarpa são medidas promissoras para o controle da morte prematura de plantas. Plantas enxertadas sobre P. giberti apresentaram menor vigor a partir dos 180 dias, menor porte vegetativo, frutos com menor diâmetro e peso e menor produtividade. / The yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg.) has been suffering with many diseases, what have been severely compromising the crop yield, determining the migration of the culture. Amongst the diseases, the premature death of plants has detached, causing crop losses the State of São Paulo. This disease has been associated with soil fungi, as Fusarium oxysporum f. passiflorae, Fusarium solani, Phytophthora sp, and also bacteria, as Xanthomonas axonopodis f. passiflorae. Measures to control premature death of plants are preventive, and, once affected by pathogens, the plant will die because there is no curative control. The application of chemical defensives has not been effective to solve the plants premature deaths. The grafting of yellow passion fruit in resistant rootstock is a promising technique for this disease control. This work aimed to evaluate the performance of yellow passion fruit grafted on three rootstocks, in two systems of grafting, cultivated in sites with or without disease history. Three experiments were carried out, two in nursery and one in the field, in Adamantina, SP, from December 2005 to July 2006. It was concluded that the two methods of grafting utilized were successful for the three species of Passiflora. The use of P. giberti and P. alata as rootstocks for P. edulis f. flavicarpa are promising measures for the control of premature death of plants. Plants grafting on P. giberti presented lower vigor form the 180 days, lower vegetative growth, fruits with lower diameter and weight and lower yield.

Made available in DSpace on 2014-11-10T11:09:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-05-23Bitstream added on 2014-11-10T11:57:42Z : No. of bitstreams: 1 000790896.pdf: 1624782 bytes, checksum: 86f691c9d97bad42fa2588df159f183a (MD5) / O Vírus da Febre amarela (YFV) causa doença com considerável morbidade e mortalidade nas regiões tropicais. Diversos vírus possuem estratégias para a alteração dos processos celulares. Mecanismos de splicing celulares são essenciais para diversificar a expressão dos genes e podem aumentar seu potencial de gerar proteínas. A replicação de YFV e as interações entre proteínas virais e celulares não são totalmente conhecidas. A proteína celular hSlu7 possui sinal de localização nuclear e tem um papel importante nas reações catalíticas do segundo passo do splicing. Estudos demonstram que sob infecção de YFV hSlu7 transloca para o citoplasma. A translocação de proteínas entre o citoplasma e o núcleo pode representar um mecanismo viral da regulação da expressão gênica. Este estudo teve como objetivo a caracterização da interação entre a proteína hSlu7 e NS5 viral, bem como estudar os efeitos de interações sobre os mecanismos de splicing alternativo após a infecção de YFV. Para identificar interação de NS5 de YFV com hSlu7 foi realizado ensaio de co-imunoprecipitação. Para verificar alteração de splicing celular foram utilizados replicons pEGFP-ADAR, pI12-IL7R, pEFGP-FGFR2, bem como a alteração de isoformas de XBP-1 endógenos. Os resultados indicam que NS5 de YFV interage com proteína hSlu7 e que sua interação pode influenciar no metabolismo RNA celular. YFV demonstrou exercer modulação no splicing celular, a avaliação de replicons sugerem que em splicing dependente de hSlu7, bem como a independente ocorre uma regulação viral atuando sobre sítios de splicing fraco e que a interação hSlu7-NS5 pode alterar direta e indiretamente a regulação trans-acting / Yellow fever virus (YFV) causes disease with significant morbidity and mortality in tropical regions. Several viral strategies are avail for recruitment and alteration of the biochemical cellular processes. Cellular splicing mechanisms are essential to diversify the gene expression and increase it’s proteomic potential. Replication of YFV and the interactions between viral and cellular proteins are unknown. The cellular protein hSlu7 has an nuclear localization and an important role in the second catalytic reaction step of the alternative splicing. In our study group demonstrated that under YFV infection hSlu7 translocates to the cytoplasm. The translocation of proteins between nucleus and cytoplasm may represent a viral mechanism of cellular gene expression regulation, interference in the protein availability of the alternative splicing and viral replication control. This study aimed to characterize the interaction between the viral protein hSlu7 and NS5, as well as studying the effects of interactions on the mechanisms of alternative splicing after YFV infection. To identify interaction with YFV NS5 hSlu7 was conducted co-immunoprecipitation assay. To verify changes in cellular splicing replicons were used pEGFP-ADAR, PI12-IL7R, pEFGP-FGFR2 as well as the change of isoforms of XBP-1 endogenous.The results indicated that YFV NS5 protein interacts with hSlu7 and that their interaction may influence cellular metabolism RNA. YFV perform modulation on cellular splicing, the evaluation of replicons suggest that in hSlu7 splicing dependent and independent regulation occurs viral acting on weak splice sites and that the interaction hSlu7-NS5 can change directly or indirectly to regulate trans- acting

abstract: Despite the approval of a Dengue virus (DV) vaccine in five endemic countries, dengue prevention would benefit from an immunization strategy highly immunogenic in young infants and not curtailed by viral interference. Problematically, infants younger than 9 year of age, whom are particularly prone to Dengue severe infection and death, cannot be immunized using current approved DV vaccine. The most important issues documented so far are the lack of efficiency and enhancement of the disease in young seronegative recipients, as well as uneven protection against the four DV serotypes. Based on data from clinical trials that showed enhanced performance of dengue vaccines when the host has previous anti-flaviviral immunity, I proposed here an attractive solution to complement the current vaccine: a recombinant measles vaccine vectoring dengue protective antigens to be administered to young infants. I hypothesized that recombinant measles virus expressing Dengue 2 and 4 antigens would successfully induce neutralizing responses against DV2 and 4 and the vaccine cocktail of this recombinant measles can prime anti-flaviviral neutralizing immunity. For this dissertation, I generated and performed preclinical immune assessment for four novel Measles-Dengue (MV-DV) vaccine candidates. I generated four MVs expressing the pre membrane (prM) and full length or truncated (90%) forms of the major envelope (E) from DV2 and DV4. Two virus, MVvac2-DV2(prME)N and MVvac2-DV4(prME), expressed high levels of membrane associated full-length E, while the other two viruses, MVvac2-DV2(prMEsol)N and MVvac2-DV4(prMEsol)N, expressed and secreted truncated, soluble E protein to its extracellular environment. The last two vectored vaccines proved superior anti-dengue neutralizing responses comparing to its corresponding full length vectors. Remarkably, when MVvac2-DV2/4(prMEsol)N recombinant vaccines were combined, the vaccine cocktail was able to prime cross-neutralizing responses against DV 1 and the relatively distant 17D yellow fever virus attenuated strain. Thus, I identify a promising DV vaccination strategy, MVvac2-DV2/4(prMEsol)N, which can prime broad neutralizing immune responses by using only two of the four available DV serotypes. The current MV immunization scheme can be advantageus to prime broad anti-flaviviral neutralizing immunity status, which will be majorly boosted by subsequent chimeric Dengue vaccine approaches. / Dissertation/Thesis / Doctoral Dissertation Microbiology 2016

Electromagnetic induction in the continental Bohai Bay coastal region of China and the island region of Japan is studied with the aid of laboratory analogue models. Detailed model measurements of the electric (Ex, Ey) and magnetic (Bx, By, Bz) field components are presented for an approximately uniform overhead horizontal source field for E- and B-polarizations. With the aid of 2D numerical models, criteria are developed for permitting approximate removal of the coast effect responses in field site measurements in coastal regions. For the Bohai Bay laboratory analogue model, large anomalous in-phase and quadrature model magnetic fields are observed over the Korea-Japan strait for E-polarization, and over the Bohai strait for B-polarization, due to current channelling through the straits. Large responses over the peninsulas in the shallow coastal areas occur at short periods but decrease abruptly with increasing period. The model induction arrows show that the induction in the local Bohai Bay is important primarily at short periods. At long periods, induction in the distant deeper Yellow Sea must be considered in any interpretation of field site measurements. In general, the analogue model results indicate that the effects of peninsulas, straits, bays and the irregularities in the coastlines play an important role in determining the electric and magnetic field responses both on-shore and off-shore for this complex coastal Bohai Bay region. For the Japan laboratory analogue model, electromagnetic field measurements are examined in detail for a series of traverses over Japan, the Korean peninsula, and the coastal region of China and the U.S.S.R. Large anomalous in-phase model magnetic fields are observed over the Korea-Japan strait for E-polarization and over Bohai strait, Tsugaru strait, and La Perouse strait for B-polarization due to off-shore current channelling. The significant responses observed at short periods over the peninsulas in the shallow coastal areas decrease with increasing period. Large gradients in the in-phase BZ are observed over all regions of Japan for E-polarization for both short and long periods due to the effects of induced currents in the surrounding oceans. Thus, induction arrow responses over all regions of Japan show the dominant effects of the ocean. The 2D numerical calculations of EM induction in continental and island coastal regions for an anomalous conductor in the form of an upwelling or a depression in the conductive substratum, show that if the anomalous conductor - ocean separation distance is at least as great as the coast effect response range YR (defined in the present work to be the range where the coast effect |Bz/Byn| has decreased to a value of 0.2), then the coast effect can be removed by vector subtraction to yield a response, approximately that of the anomalous conductor alone. For a given period (in the range 5 -120 min), YR is found to increase with increasing ocean depth, conductive substratum depth, and island width. Further, the dependan.ee on period is found to vary from model to model, but the general trend is for YR to decrease with increasing period, on account of the increasing importance of the underlying conductive substratum through the skin depth effect in the host. Empirical curves are presented showing how the response range depends on the ocean depth, the conductive substratum depth, the island width and the period. Coast effect response values for 3D laboratory analogue models are employed to approximately remove the geomagnetic coast effects in field measurements for some coastal sites in the Bohai Bay continental region of China and the island regions of Japan. The validity of the subtraction is examined for several models of conductive anomalies at sufficiently large anomalous conductor - ocean ranges to satisfy the response range criteria developed for 2D numerical models. The resulting interpretation of field site measurements in complex coastal regions is discussed. With the coast effect removed though subtraction of the model arrows from field site results available in the Bohai Bay region, the resulting difference arrows indicate a N-S striking conductor to the west of Bohai Bay. These difference arrows, as well as the 2D numerical calculations, support the premise of such a conductor, in the form of an upwelling in the conductive substratum (with conductivity 0.5Sm-1 at 80 km depth), situated at about 150 km from the Bohai Bay coastline to account for the field site observations. A comparison of laboratory analogue model and field site MT results at two sites west of Bohai Bay shows that the analogue model apparent resistivities are about an order of magnitude greater than the field site apparent resistivities. This result also supports the model of a conductive anomaly, in addition to the conductive substratum at 80 km depth. Laboratory analogue model measurements are employed to subtract the ocean effect in field measurements to yield difference arrow for these complex island regions of Japan ( the Kii Peninsula region, the central Honshu region and the regions of northern Honshu, Hokkaido and Tsugaru strait). These difference arrows as well as the 2-D numerical model result for the Kii Peninsula region, the central Honshu region, and the northern Honshu region support the premise of two conductive anomalies (with conductivity 0.5Sm-1), one beneath the Pacific Ocean and one beneath the Japan Sea at a depth of 30 km. Further, the difference arrows over the entire Japan region suggest that the two conductors roughly follow the general trend of the island arc, and eventually may be connected by an E-W striking conductor beneath Tsugaru strait to the north. / Graduate

Made available in DSpace on 2014-06-11T19:27:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-12-04Bitstream added on 2014-06-13T20:56:09Z : No. of bitstreams: 1 rodrigues_to_me_araca.pdf: 1242136 bytes, checksum: f935eebe57d980d63dfba1575e1ab773 (MD5) / Considerando que a práxis docente na educação básica abrange educação em saúde, esta pesquisa teve como objetivo avaliar o resultado de ações de educação em saúde oferecidas por meio de um curso de formação continuada, com atividades a distância por meio do Ambiente Virtual de Aprendizagem TelEduc e encontros presenciais, para docentes da rede municipal de Ensino Fundamental I de Araçatuba-SP. O curso foi organizado em duas fases, sendo a Fase I subdivida em módulos de temas como ambientação tecnológica, doenças transmitidas por vetores, doenças transmitidas por cães e gatos, higiene de alimentos, posse responsável e educação em saúde na escola e a Fase II para elaboração e aplicação de projetos educativos. Conhecimentos específicos dos docentes sobre dengue, febre amarela e leishmaniose, foram comparados antes e após o curso, observando-se um crescimento estatisticamente significante no conhecimento sobre estas doenças (p<0,0001) com destaque para as questões relativas ao agente etiológico das doenças, sintomas no homem e medidas de prevenção. A prática de aplicação do projeto educativo teve como critério as ações de multiplicação feitas pelo público-alvo. A experiência de multiplicação dos professores envolveu escolares, que diagnosticaram os problemas por meio de mapa falante e promoveram ações educativas no bairro, destacando-se, dentre outras: peças teatrais, atividades artísticas, mutirão de limpeza e elaboração e distribuição de panfletos em passeata. Conclui-se que a formação continuada de docentes, por meio de cursos semipresenciais, proporciona conhecimentos, impulsiona-os a motivar os educandos a adotar medidas de controle de vetores, cuidar do meio ambiente e sensibilizar a comunidade, colaborando para o controle de doenças / Considering that the educational praxis on basic education level also includes health education, our purpose was to evaluate the results of an online semi-present continued formation program, directed to teachers from Fundamental I Education level in Araçatuba-SP. The course was organized in two parts, with the Part I divided in themes as technological environment, vector borne diseases, diseases transmitted by dogs and cats, food hygiene, responsible ownership and health education in school and Part II reserved for elaboration and application of educative projects. The specific knowledge about dengue, yellow fever and leishmaniasis was compared before and after the attendance to a course with distance and present classes. Tests showed a statistically significant positive inversion in the knowledge about the studied diseases (p<0.0001) after the course, with emphasis to questions related to diseases etiological agents, symptoms in man and prevention measures. The application of practical educative projects was evaluated by multiplicative actions involving target public. The experience of knowledge multiplication included teachers and students which detected the problems by using talking maps and promoted education actions in their neighborhood, specially mimic plays artistic activities, group cleaning activities and elaboration and distribution of educational material in public demonstrations. We conclude that continuing education using semi-present courses, provides new knowledge to the teachers, impelling them to motivate their students to adopt vectors control measures and environmental cares and to sensitive the community, collaborating this way to control diseases

Made available in DSpace on 2014-06-11T19:27:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-02-13Bitstream added on 2014-06-13T19:35:40Z : No. of bitstreams: 1 konta_em_me_arafcf.pdf: 565336 bytes, checksum: 6c84917b1244d00658ee92bffe42aa3f (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Universidade Estadual Paulista (UNESP) / Os antioxidantes, principalmente os encontrados na dieta, são agentes responsáveis pela inibição e redução dos danos oxidativos causados pelas espécies reativas nas células. A cisplatina (cDDP), um potente agente antineoplásico utilizado com frequência no tratamento de tumores sólidos, tem seu uso clínico limitado devido aos efeitos adversos como nefrotoxicidade, genotoxicidade e supressão da medula óssea. Como a geração de espécies reativas pode resultar em danos ao DNA pela ação destas espécies ou indiretamente via produtos de degradação da peroxidação lipídica, nosso estudo avaliou o possível efeito protetor da adição da polpa de maracujá amarelo na dieta de ratos espontaneamente hipertensos (SHR) e Wistar normotensos, contra a nefrotoxicidade e genotoxicidade induzida pela cDDP. Os resultados obtidos nas condições experimentais estabelecidas neste trabalho mostraram que a cDDP promoveu um aumento dos níveis das substâncias reativas aos ácido tiobarbitúrico, bem como decréscimo nos níveis de glutationa nos rins dos animais SHR, reforçando a hipótese que a peroxidação lipídica está relacionada com o mecanismo de nefrotoxicidade. O tratamento com doses múltiplas da polpa de maracujá amarelo não alterou os outros parâmetros da função renal no período analisado. Embora o efeito protetor da polpa de maracujá contra os danos induzidos ao DNA em células renais e hepáticas não tenha sido evidente nos resultados obtidos pelo Ensaio do Cometa, a polpa foi eficaz na redução de micronúcleos induzidos pela cDDP. Assim, umas das hipóteses para a redução da pressão arterial sistólica observada neste trabalho, poder ser atribuída à presença de alguns compostos fenólicos e outros ainda não identificados na polpa. Visto que, a interação entre os fitoquímicos presentes na polpa de maracujá demonstraram eficácia na redução da mutagenicidade e efeitos protetores antioxidantes. / The antioxidants, particularly those found in the diet, are responsible for inhibiting and reducing the damage caused by reactive species in the cells. Cisplatin (cDDP), a potent anticancer agent often used in the treatment of solid tumors, has limited clinical use due to adverse effects such as nephrotoxicity, genotoxicity and myelosuppression. As the generation of reactive species can result in DNA damage by direct action of this or indirectly by degradation products of the lipid peroxidation, our study evaluated the potential protector of adding yellow passion fruit pulp to the diet of spontaneously hypertensive rats (SHR) and normotensive wistar rats, against the nephrotoxicity and genotoxicity induced by cDDP. The results showed that the cDDP promoted an increase in the levels of thiobarbituric acid reactive substances and a decrease in the levels of glutathione in the kidneys of the animals, reinforcing the hypothesis that lipid peroxidation be related to the mechanism of nephrotoxicity. The treatment with multiple doses of passion fruit pulp did not change the others parameters of renal function in the schedule. Although the protective effect of passion fruit pulp against the damage caused to the DNA of kidney and hepatic cells has not been evident in the results obtained by Comet assay, the pulp was effective in the reduction of micronuclei induced by cDDP. One of the hypotheses for the reduction in systolic blood pressure observed in the present study attributed this effect to the presence of some phenolic compounds and others not yet identified in the passion fruit pulp. Furthermore, the interaction between the phytochemicals of the pulp demonstrated effectiveness in reducing the mutagenic and antioxidant effects.

Orientador: Maurício Lacerda Nogueira / Banca: Carlos Eduardo Calzavara Silva / Banca: Fátima Pereira de Souza / Resumo: O Vírus da Febre amarela (YFV) causa doença com considerável morbidade e mortalidade nas regiões tropicais. Diversos vírus possuem estratégias para a alteração dos processos celulares. Mecanismos de splicing celulares são essenciais para diversificar a expressão dos genes e podem aumentar seu potencial de gerar proteínas. A replicação de YFV e as interações entre proteínas virais e celulares não são totalmente conhecidas. A proteína celular hSlu7 possui sinal de localização nuclear e tem um papel importante nas reações catalíticas do segundo passo do splicing. Estudos demonstram que sob infecção de YFV hSlu7 transloca para o citoplasma. A translocação de proteínas entre o citoplasma e o núcleo pode representar um mecanismo viral da regulação da expressão gênica. Este estudo teve como objetivo a caracterização da interação entre a proteína hSlu7 e NS5 viral, bem como estudar os efeitos de interações sobre os mecanismos de splicing alternativo após a infecção de YFV. Para identificar interação de NS5 de YFV com hSlu7 foi realizado ensaio de co-imunoprecipitação. Para verificar alteração de splicing celular foram utilizados replicons pEGFP-ADAR, pI12-IL7R, pEFGP-FGFR2, bem como a alteração de isoformas de XBP-1 endógenos. Os resultados indicam que NS5 de YFV interage com proteína hSlu7 e que sua interação pode influenciar no metabolismo RNA celular. YFV demonstrou exercer modulação no splicing celular, a avaliação de replicons sugerem que em splicing dependente de hSlu7, bem como a independente ocorre uma regulação viral atuando sobre sítios de splicing fraco e que a interação hSlu7-NS5 pode alterar direta e indiretamente a regulação trans-acting / Abstract: Yellow fever virus (YFV) causes disease with significant morbidity and mortality in tropical regions. Several viral strategies are avail for recruitment and alteration of the biochemical cellular processes. Cellular splicing mechanisms are essential to diversify the gene expression and increase it's proteomic potential. Replication of YFV and the interactions between viral and cellular proteins are unknown. The cellular protein hSlu7 has an nuclear localization and an important role in the second catalytic reaction step of the alternative splicing. In our study group demonstrated that under YFV infection hSlu7 translocates to the cytoplasm. The translocation of proteins between nucleus and cytoplasm may represent a viral mechanism of cellular gene expression regulation, interference in the protein availability of the alternative splicing and viral replication control. This study aimed to characterize the interaction between the viral protein hSlu7 and NS5, as well as studying the effects of interactions on the mechanisms of alternative splicing after YFV infection. To identify interaction with YFV NS5 hSlu7 was conducted co-immunoprecipitation assay. To verify changes in cellular splicing replicons were used pEGFP-ADAR, PI12-IL7R, pEFGP-FGFR2 as well as the change of isoforms of XBP-1 endogenous.The results indicated that YFV NS5 protein interacts with hSlu7 and that their interaction may influence cellular metabolism RNA. YFV perform modulation on cellular splicing, the evaluation of replicons suggest that in hSlu7 splicing dependent and independent regulation occurs viral acting on weak splice sites and that the interaction hSlu7-NS5 can change directly or indirectly to regulate trans- acting / Mestre

Resumo: A cajazeira quando propagada por sementes apresenta elevada variabilidade genética quanto ao porte, arquitetura, formato da copa, fenologia da planta, características físico-químicas de folhas e frutos e fase juvenil bastante ampla, características não desejáveis para a exploração comercial desta cultura. O método de seleção massal para escolha de genótipos superiores tem sido utilizado com bastante sucesso em fruteiras. Considerando as poucas informações existentes sobre cajazeira, o grande potencial de exploração agroindustrial que esta fruteira apresenta e a necessidade de se preservar o amplo e ainda pouco conhecido patrimônio genético, o presente trabalho teve como objetivo avaliar a variabilidade genética entre 113 genótipos de cajazeiras, pés-francos, através da mensuração das características físicas e químicas para identificar possíveis genótipos com potencial superior e características agronômicas adequadas para recomendação de cultivo. Os dados foram submetidos à análise de variância, pelo teste F, teste de Scott-Knot para as características significativas. As análises de agrupamentos foram realizadas utilizando-se programa para estatísticas multivariadas. Os resultados obtidos permitem afirmar que: todos os genótipos estudados apresentaram elevada variabilidade para as características físicas e químicas, confirmando a possibilidade de obtenção e utilização como matrizes, com atributos superiores; as melhores características tecnológicas e físicas dos frutos foram encontradas nos genótipos F18P2, F11P6, F5P3, F4P11, F15P11 e F14P7; os fatores de produção, número de panículas com frutos e o total de frutos por panícula, são determinantes para a seleção de genótipos de cajazeiras e que os genótipos F14P9 e F14P8 apresentaram as melhores características agronômicas para seleção de genótipos com melhor produtividade / Abstract: The yellow mombin when propagated by seed has high genetic variability of size, architecture, form of canopy, plant phenology, physical and chemical characteristics of leaves and fruits and juvenile phase, undesirable characteristics for commercial exploration of this crop. The method of mass selection in order to choose superior genotypes has been used quite successfully in fruit crops. Considering the little information available concerning the yellow mombin, the large potential for agroindustrial exploration that this fruit has and the need to preserve the broad and still little genetic heritage, this study aims to evaluate the genetic variability between the 113 genotypes of the yellow mombin, seedlings, by measuring the physical, physicochemical characteristics and identify these possible genotypes with higher potential and agronomic traits suitable for cultivation recommendation. The data were subjected to analysis of variance by F test, Scott-Knott test for significant features. The cluster analysis was performed have revealed that all genotypes showed high variability for the possibility of obtaining matrices with superior attributes; the best technological and physical characteristics of fruits were found in F18P2, F11P6, F5P3, F4P11, F15P11 and F14P7 genotypes; factors of production, number of panicles with fruit and total fruit per panicle, are decisive for the selection of yellow mombin genotypes and F14P9 and F14P8 showed the best agronomic traits for selecting genotypes with improved productivity / Orientador: Antonio Baldo Geraldo Martins / Coorientador: José Carlos Barbosa / Banca: José Antonio Alberto da Silva / Banca: Leila Trevizan Braz / Banca: Simone Rodrigues da Silva / Banca: Luiz Evaldo de Moura Pádua / Doutor

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Este texto é uma fabulação que cumpre papel de revelar um processo de trabalho em arte ao longo de dois anos. Trata-se de um percurso teórico-poético percorrido a partir da descoberta/invenção de um ateliê de criação em meio a minha residência. Um quadrado de 2x2 metros demarcado por sobreposição = fita amarela. Instaurado em divergentes contextos paisagísticos = casa, quarto de hotel, estúdio de dança, galeria de arte, palco, cidade. Trata de habitar os mesmos friccionando esfera de vivência pessoal e sítio específico de acontecimento. No texto, tem lugar reflexões pessoais a partir do trabalho, articulada a outros artistas (como Lygia Clark, Angel e Klauss Vianna, Antonin Artaud, Yves Klein, Min Tanaka, Tadashi Endo) e teóricos (dentre eles Hannah Arendt e Michel Foucault), compondo diferentes tons de fala produzidos ao longo desse período de trabalho / This essay is a fabulation that presents an art work over two years. It is a theoretical-poetic way, coursed from the discovery/invention of a creation studio inside my residence. A square of 2x2m made by superposition = yellow tape, done in different landscape contexts = house, hotel room, dance studio, art gallery, theater stage, city. The intention is to inhabit it rubbing a sphere of personal experiences and a specific site of happening. In the text, personal reflections take place from the art work, articulated with other artists (as Lygia Clark, Angel e Klauss Vianna, Antonin Artaud, Yves Klein, Min Tanaka, Tadashi Endo) and theorists (Hannah Arendt e Michael Foucault), composing different kinds of speech produced along this work period

A new method was developed for testing antiviral compounds against plant viruses based on rapidly growing brassicas in vitro on liquid medium. While using ribavirin as a standard for comparison, phytotoxicity and ability of the acyclic nucleotide analogues(R)-PMPA, PMEA, PMEDAP, and (S)-HPMPC to eliminate ssRNA Turnip yellow mosaic virus (TYMV) were evaluated by this method. Double antibody sandwich ELISA was used for relative quantification of viral protein in plants. Ribavirin had the most powerful antiviral effect against TYMV. On the other hand, (R)-PMPA and PMEA had no antiviral effect and almost no phytotoxicity compared to the control. (S)-HPMPC and PMEDAP showed moderate antiviral effect, accompanied by higher phytotoxicity.